AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0547490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0547490 MtrunA17_Chr4g0029791 97.872 94 2 0 1 94 1 94 6.33e-65 190
Msa0547490 MtrunA17_Chr2g0326261 45.361 97 27 4 1 94 1 74 1.80e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0547490 AT5G60400.3 40.449 89 51 1 6 94 5 91 9.26e-12 56.2

Find 24 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTCCTCTCTGTCTTTC+TGG 0.075036 4_1:-40777941 Msa0547490:CDS
GTTTAACATGGACTGCATTT+TGG 0.222924 4_1:+40777979 None:intergenic
AAAATCAAATATGCATCTTT+TGG 0.233478 4_1:-40777340 Msa0547490:three_prime_UTR
ACTTACTAGAATGTTAATTT+AGG 0.250075 4_1:+40777444 None:intergenic
AAAAGCTGCTTAGTTAGATT+TGG 0.304540 4_1:-40777577 Msa0547490:exon
CGAAGTTTCACCCTATTCAT+TGG 0.395651 4_1:+40777397 None:intergenic
TGATGATGATCTTGAAGATC+TGG 0.442771 4_1:-40777649 Msa0547490:CDS
TTCGTACGGACATATTTGTA+TGG 0.453259 4_1:-40777378 Msa0547490:three_prime_UTR
AGTGCATGCAAATCAAGAAA+AGG 0.472454 4_1:-40777715 Msa0547490:CDS
GACTGATGTGACCAATGAAT+AGG 0.482367 4_1:-40777408 Msa0547490:three_prime_UTR
TTAAACCTTCGTGCTACTCA+TGG 0.498021 4_1:-40777962 Msa0547490:CDS
ACTGATGTGACCAATGAATA+GGG 0.501250 4_1:-40777407 Msa0547490:three_prime_UTR
ACCGAAGAAGAAAAGATATG+AGG 0.524381 4_1:-40777749 Msa0547490:CDS
GTGCATGCAAATCAAGAAAA+GGG 0.524986 4_1:-40777714 Msa0547490:CDS
CTGTCTCTTACTTATCTCAC+AGG 0.529035 4_1:-40777609 Msa0547490:CDS
TACTCTTTGGAAAGAGCAAG+AGG 0.575505 4_1:+40777908 None:intergenic
AACATTCTAGTAAGTTCAGA+CGG 0.600784 4_1:-40777436 Msa0547490:three_prime_UTR
TTTGGAAAGAGCAAGAGGGT+TGG 0.601098 4_1:+40777913 None:intergenic
AAAAGGATGGGAAATAAAGG+AGG 0.624688 4_1:+40778030 None:intergenic
GAATAGGGTGAAACTTCGTA+CGG 0.636390 4_1:-40777392 Msa0547490:three_prime_UTR
ACTCTTTGGAAAGAGCAAGA+GGG 0.667434 4_1:+40777909 None:intergenic
AGTAGCACGAAGGTTTAACA+TGG 0.672060 4_1:+40777967 None:intergenic
GTGATTCCAGAAAGACAGAG+AGG 0.712435 4_1:+40777935 None:intergenic
GAACTCCATGAGTAGCACGA+AGG 0.732005 4_1:+40777957 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATTAATTTACTCTT+TGG + chr4_1:40777550-40777569 None:intergenic 10.0%
!! AAATAAATTAATTTACTCTT+TGG + chr4_1:40777550-40777569 None:intergenic 10.0%
!! TGATATATGTTTGAATTTGA+TGG - chr4_1:40777651-40777670 Msa0547490:CDS 20.0%
!! ACTTACTAGAATGTTAATTT+AGG + chr4_1:40778001-40778020 None:intergenic 20.0%
!!! ATGCATATTTGATTTTGATT+TGG + chr4_1:40778098-40778117 None:intergenic 20.0%
!!! AAAATCAAATATGCATCTTT+TGG - chr4_1:40778102-40778121 Msa0547490:five_prime_UTR 20.0%
!! TGATATATGTTTGAATTTGA+TGG - chr4_1:40777651-40777670 Msa0547490:CDS 20.0%
!! ACTTACTAGAATGTTAATTT+AGG + chr4_1:40778001-40778020 None:intergenic 20.0%
!!! ATGCATATTTGATTTTGATT+TGG + chr4_1:40778098-40778117 None:intergenic 20.0%
!!! AAAATCAAATATGCATCTTT+TGG - chr4_1:40778102-40778121 Msa0547490:five_prime_UTR 20.0%
!! TTTTCTAGGTAAAGGAAAAA+AGG + chr4_1:40777432-40777451 None:intergenic 25.0%
!!! GTTTTGTTTTTTCTAGGTAA+AGG + chr4_1:40777440-40777459 None:intergenic 25.0%
!!! TGGTTTGTTTTGTTTTTTCT+AGG + chr4_1:40777446-40777465 None:intergenic 25.0%
!! TCAAAGAAACAATGATTTTG+TGG - chr4_1:40777760-40777779 Msa0547490:CDS 25.0%
!! TTTTCTAGGTAAAGGAAAAA+AGG + chr4_1:40777432-40777451 None:intergenic 25.0%
!!! GTTTTGTTTTTTCTAGGTAA+AGG + chr4_1:40777440-40777459 None:intergenic 25.0%
!!! TGGTTTGTTTTGTTTTTTCT+AGG + chr4_1:40777446-40777465 None:intergenic 25.0%
!! TCAAAGAAACAATGATTTTG+TGG - chr4_1:40777760-40777779 Msa0547490:CDS 25.0%
GAAAAAAGGATGGGAAATAA+AGG + chr4_1:40777418-40777437 None:intergenic 30.0%
TAGGTAAAGGAAAAAAGGAT+GGG + chr4_1:40777427-40777446 None:intergenic 30.0%
! TCCTCATATCTTTTCTTCTT+CGG + chr4_1:40777697-40777716 None:intergenic 30.0%
! AATGATTTTGTGGAGATGAA+AGG - chr4_1:40777770-40777789 Msa0547490:intron 30.0%
AAAAGCTGCTTAGTTAGATT+TGG - chr4_1:40777865-40777884 Msa0547490:intron 30.0%
! TCTAGATCACTTTCATTTTC+TGG - chr4_1:40777902-40777921 Msa0547490:intron 30.0%
AACATTCTAGTAAGTTCAGA+CGG - chr4_1:40778006-40778025 Msa0547490:five_prime_UTR 30.0%
GAAAAAAGGATGGGAAATAA+AGG + chr4_1:40777418-40777437 None:intergenic 30.0%
TAGGTAAAGGAAAAAAGGAT+GGG + chr4_1:40777427-40777446 None:intergenic 30.0%
! TCCTCATATCTTTTCTTCTT+CGG + chr4_1:40777697-40777716 None:intergenic 30.0%
! AATGATTTTGTGGAGATGAA+AGG - chr4_1:40777770-40777789 Msa0547490:intron 30.0%
AAAAGCTGCTTAGTTAGATT+TGG - chr4_1:40777865-40777884 Msa0547490:intron 30.0%
! TCTAGATCACTTTCATTTTC+TGG - chr4_1:40777902-40777921 Msa0547490:intron 30.0%
AACATTCTAGTAAGTTCAGA+CGG - chr4_1:40778006-40778025 Msa0547490:five_prime_UTR 30.0%
AAAAGGATGGGAAATAAAGG+AGG + chr4_1:40777415-40777434 None:intergenic 35.0%
CTAGGTAAAGGAAAAAAGGA+TGG + chr4_1:40777428-40777447 None:intergenic 35.0%
!! GTTTAACATGGACTGCATTT+TGG + chr4_1:40777466-40777485 None:intergenic 35.0%
GTGAGATCATAGATCAAAAG+AGG + chr4_1:40777592-40777611 None:intergenic 35.0%
!! ATGTTTGAATTTGATGGTGC+AGG - chr4_1:40777657-40777676 Msa0547490:CDS 35.0%
ACCGAAGAAGAAAAGATATG+AGG - chr4_1:40777693-40777712 Msa0547490:CDS 35.0%
AGTGCATGCAAATCAAGAAA+AGG - chr4_1:40777727-40777746 Msa0547490:CDS 35.0%
GTGCATGCAAATCAAGAAAA+GGG - chr4_1:40777728-40777747 Msa0547490:CDS 35.0%
! TGATGATGATCTTGAAGATC+TGG - chr4_1:40777793-40777812 Msa0547490:intron 35.0%
ACTGATGTGACCAATGAATA+GGG - chr4_1:40778035-40778054 Msa0547490:five_prime_UTR 35.0%
TTCGTACGGACATATTTGTA+TGG - chr4_1:40778064-40778083 Msa0547490:five_prime_UTR 35.0%
AAAAGGATGGGAAATAAAGG+AGG + chr4_1:40777415-40777434 None:intergenic 35.0%
CTAGGTAAAGGAAAAAAGGA+TGG + chr4_1:40777428-40777447 None:intergenic 35.0%
!! GTTTAACATGGACTGCATTT+TGG + chr4_1:40777466-40777485 None:intergenic 35.0%
GTGAGATCATAGATCAAAAG+AGG + chr4_1:40777592-40777611 None:intergenic 35.0%
!! ATGTTTGAATTTGATGGTGC+AGG - chr4_1:40777657-40777676 Msa0547490:CDS 35.0%
ACCGAAGAAGAAAAGATATG+AGG - chr4_1:40777693-40777712 Msa0547490:CDS 35.0%
AGTGCATGCAAATCAAGAAA+AGG - chr4_1:40777727-40777746 Msa0547490:CDS 35.0%
GTGCATGCAAATCAAGAAAA+GGG - chr4_1:40777728-40777747 Msa0547490:CDS 35.0%
! TGATGATGATCTTGAAGATC+TGG - chr4_1:40777793-40777812 Msa0547490:intron 35.0%
ACTGATGTGACCAATGAATA+GGG - chr4_1:40778035-40778054 Msa0547490:five_prime_UTR 35.0%
TTCGTACGGACATATTTGTA+TGG - chr4_1:40778064-40778083 Msa0547490:five_prime_UTR 35.0%
AGTAGCACGAAGGTTTAACA+TGG + chr4_1:40777478-40777497 None:intergenic 40.0%
! TTAAACCTTCGTGCTACTCA+TGG - chr4_1:40777480-40777499 Msa0547490:three_prime_UTR 40.0%
! ACTCTTTGGAAAGAGCAAGA+GGG + chr4_1:40777536-40777555 None:intergenic 40.0%
! TACTCTTTGGAAAGAGCAAG+AGG + chr4_1:40777537-40777556 None:intergenic 40.0%
!! TTGAATTTGATGGTGCAGGA+TGG - chr4_1:40777661-40777680 Msa0547490:CDS 40.0%
CTGTCTCTTACTTATCTCAC+AGG - chr4_1:40777833-40777852 Msa0547490:intron 40.0%
GACTGATGTGACCAATGAAT+AGG - chr4_1:40778034-40778053 Msa0547490:five_prime_UTR 40.0%
CGAAGTTTCACCCTATTCAT+TGG + chr4_1:40778048-40778067 None:intergenic 40.0%
GAATAGGGTGAAACTTCGTA+CGG - chr4_1:40778050-40778069 Msa0547490:five_prime_UTR 40.0%
AGTAGCACGAAGGTTTAACA+TGG + chr4_1:40777478-40777497 None:intergenic 40.0%
! TTAAACCTTCGTGCTACTCA+TGG - chr4_1:40777480-40777499 Msa0547490:three_prime_UTR 40.0%
! ACTCTTTGGAAAGAGCAAGA+GGG + chr4_1:40777536-40777555 None:intergenic 40.0%
! TACTCTTTGGAAAGAGCAAG+AGG + chr4_1:40777537-40777556 None:intergenic 40.0%
!! TTGAATTTGATGGTGCAGGA+TGG - chr4_1:40777661-40777680 Msa0547490:CDS 40.0%
CTGTCTCTTACTTATCTCAC+AGG - chr4_1:40777833-40777852 Msa0547490:intron 40.0%
GACTGATGTGACCAATGAAT+AGG - chr4_1:40778034-40778053 Msa0547490:five_prime_UTR 40.0%
CGAAGTTTCACCCTATTCAT+TGG + chr4_1:40778048-40778067 None:intergenic 40.0%
GAATAGGGTGAAACTTCGTA+CGG - chr4_1:40778050-40778069 Msa0547490:five_prime_UTR 40.0%
GTGATTCCAGAAAGACAGAG+AGG + chr4_1:40777510-40777529 None:intergenic 45.0%
TTTGGAAAGAGCAAGAGGGT+TGG + chr4_1:40777532-40777551 None:intergenic 45.0%
GTGATTCCAGAAAGACAGAG+AGG + chr4_1:40777510-40777529 None:intergenic 45.0%
TTTGGAAAGAGCAAGAGGGT+TGG + chr4_1:40777532-40777551 None:intergenic 45.0%
GAACTCCATGAGTAGCACGA+AGG + chr4_1:40777488-40777507 None:intergenic 50.0%
GGAGTTCCTCTCTGTCTTTC+TGG - chr4_1:40777501-40777520 Msa0547490:three_prime_UTR 50.0%
GAACTCCATGAGTAGCACGA+AGG + chr4_1:40777488-40777507 None:intergenic 50.0%
GGAGTTCCTCTCTGTCTTTC+TGG - chr4_1:40777501-40777520 Msa0547490:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 40777338 40778126 40777338 ID=Msa0547490;Name=Msa0547490
chr4_1 mRNA 40777338 40778126 40777338 ID=Msa0547490-mRNA-1;Parent=Msa0547490;Name=Msa0547490-mRNA-1;_AED=0.00;_eAED=0.00;_QI=130|1|1|1|1|1|2|249|94
chr4_1 exon 40777338 40777785 40777338 ID=Msa0547490-mRNA-1:exon:10406;Parent=Msa0547490-mRNA-1
chr4_1 exon 40777911 40778126 40777911 ID=Msa0547490-mRNA-1:exon:10405;Parent=Msa0547490-mRNA-1
chr4_1 five_prime_UTR 40777997 40778126 40777997 ID=Msa0547490-mRNA-1:five_prime_utr;Parent=Msa0547490-mRNA-1
chr4_1 CDS 40777911 40777996 40777911 ID=Msa0547490-mRNA-1:cds;Parent=Msa0547490-mRNA-1
chr4_1 CDS 40777587 40777785 40777587 ID=Msa0547490-mRNA-1:cds;Parent=Msa0547490-mRNA-1
chr4_1 three_prime_UTR 40777338 40777586 40777338 ID=Msa0547490-mRNA-1:three_prime_utr;Parent=Msa0547490-mRNA-1
Gene Sequence

>Msa0547490

ATGCAGTCCATGTTAAACCTTCGTGCTACTCATGGAGTTCCTCTCTGTCTTTCTGGAATCACCAACCCTCTTGCTCTTTCCAAAGAGATGGCAGATCACGACCGAAGAAGAAAAGATATGAGGAAGCAAAGATCAGTGCATGCAAATCAAGAAAAGGGTGCACAAAGTCAAAGAAACAATGATTTTGTGGAGATGAAAGGTGATGATGATCTTGAAGATCTGGATGAAATCTTGCATCAACTGTCTCTTACTTATCTCACAGGTTTATCAGCAAAAGCTGCTTAG

Protein sequence

>Msa0547490

MQSMLNLRATHGVPLCLSGITNPLALSKEMADHDRRRKDMRKQRSVHANQEKGAQSQRNNDFVEMKGDDDLEDLDEILHQLSLTYLTGLSAKAA*