AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0682620


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0682620 MtrunA17_Chr4g0029791 97.872 94 2 0 1 94 1 94 6.33e-65 190
Msa0682620 MtrunA17_Chr2g0326261 45.361 97 27 4 1 94 1 74 1.80e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0682620 AT5G60400.3 40.449 89 51 1 6 94 5 91 9.26e-12 56.2

Find 24 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTCCTCTCTGTCTTTC+TGG 0.075036 4_4:-44448771 Msa0682620:CDS
AAAATCAAATATGCATATTT+TGG 0.124229 4_4:-44448170 Msa0682620:three_prime_UTR
GTTTAACATGGACTGCATTT+TGG 0.222924 4_4:+44448809 None:intergenic
ACTTACTAGAATGTTAATTT+AGG 0.250075 4_4:+44448274 None:intergenic
AAAAGCTGCTTAGTTAGATT+TGG 0.304540 4_4:-44448407 Msa0682620:exon
CGAAGTTTCACCCTATTCAT+TGG 0.395651 4_4:+44448227 None:intergenic
TGATGATGATCTTGAAGATC+TGG 0.442771 4_4:-44448479 Msa0682620:CDS
TTCGTACGGACATATTTGTA+TGG 0.453259 4_4:-44448208 Msa0682620:three_prime_UTR
AGTGCATGCAAATCAAGAAA+AGG 0.472454 4_4:-44448545 Msa0682620:CDS
ACTGATGTGACCAATGAATA+GGG 0.474910 4_4:-44448237 Msa0682620:three_prime_UTR
AACTGATGTGACCAATGAAT+AGG 0.478716 4_4:-44448238 Msa0682620:three_prime_UTR
TTAAACCTTCGTGCTACTCA+TGG 0.498021 4_4:-44448792 Msa0682620:CDS
ACCGAAGAAGAAAAGATATG+AGG 0.524381 4_4:-44448579 Msa0682620:CDS
GTGCATGCAAATCAAGAAAA+GGG 0.524986 4_4:-44448544 Msa0682620:CDS
CTGTCTCTTACTTATCTCAC+AGG 0.529035 4_4:-44448439 Msa0682620:CDS
TACTCTTTGGAAAGAGCAAG+AGG 0.575505 4_4:+44448738 None:intergenic
AACATTCTAGTAAGTTCAGA+CGG 0.600784 4_4:-44448266 Msa0682620:three_prime_UTR
TTTGGAAAGAGCAAGAGGGT+TGG 0.601098 4_4:+44448743 None:intergenic
AAAAGGATGGGAAATAAAGG+AGG 0.624688 4_4:+44448860 None:intergenic
GAATAGGGTGAAACTTCGTA+CGG 0.636390 4_4:-44448222 Msa0682620:three_prime_UTR
ACTCTTTGGAAAGAGCAAGA+GGG 0.667434 4_4:+44448739 None:intergenic
AGTAGCACGAAGGTTTAACA+TGG 0.672060 4_4:+44448797 None:intergenic
GTGATTCCAGAAAGACAGAG+AGG 0.712435 4_4:+44448765 None:intergenic
GAACTCCATGAGTAGCACGA+AGG 0.732005 4_4:+44448787 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATTAATTTACTCTT+TGG + chr4_4:44448380-44448399 None:intergenic 10.0%
!! AAATAAATTAATTTACTCTT+TGG + chr4_4:44448380-44448399 None:intergenic 10.0%
!!! AAAATCAAATATGCATATTT+TGG - chr4_4:44448932-44448951 Msa0682620:five_prime_UTR 15.0%
!!! AAAATCAAATATGCATATTT+TGG - chr4_4:44448932-44448951 Msa0682620:five_prime_UTR 15.0%
!! TGATATATGTTTGAATTTGA+TGG - chr4_4:44448481-44448500 Msa0682620:CDS 20.0%
!! ACTTACTAGAATGTTAATTT+AGG + chr4_4:44448831-44448850 None:intergenic 20.0%
!!! ATGCATATTTGATTTTGATT+TGG + chr4_4:44448928-44448947 None:intergenic 20.0%
!! TGATATATGTTTGAATTTGA+TGG - chr4_4:44448481-44448500 Msa0682620:CDS 20.0%
!! ACTTACTAGAATGTTAATTT+AGG + chr4_4:44448831-44448850 None:intergenic 20.0%
!!! ATGCATATTTGATTTTGATT+TGG + chr4_4:44448928-44448947 None:intergenic 20.0%
!! TTTTCTAGGTAAAGGAAAAA+AGG + chr4_4:44448262-44448281 None:intergenic 25.0%
!!! GTTTTGTTTTTTCTAGGTAA+AGG + chr4_4:44448270-44448289 None:intergenic 25.0%
!!! TGGTTTGTTTTGTTTTTTCT+AGG + chr4_4:44448276-44448295 None:intergenic 25.0%
!! TCAAAGAAACAATGATTTTG+TGG - chr4_4:44448590-44448609 Msa0682620:CDS 25.0%
!! TCTAAATCACTTTCATTTTC+TGG - chr4_4:44448732-44448751 Msa0682620:intron 25.0%
!! TTTTCTAGGTAAAGGAAAAA+AGG + chr4_4:44448262-44448281 None:intergenic 25.0%
!!! GTTTTGTTTTTTCTAGGTAA+AGG + chr4_4:44448270-44448289 None:intergenic 25.0%
!!! TGGTTTGTTTTGTTTTTTCT+AGG + chr4_4:44448276-44448295 None:intergenic 25.0%
!! TCAAAGAAACAATGATTTTG+TGG - chr4_4:44448590-44448609 Msa0682620:CDS 25.0%
!! TCTAAATCACTTTCATTTTC+TGG - chr4_4:44448732-44448751 Msa0682620:intron 25.0%
GAAAAAAGGATGGGAAATAA+AGG + chr4_4:44448248-44448267 None:intergenic 30.0%
TAGGTAAAGGAAAAAAGGAT+GGG + chr4_4:44448257-44448276 None:intergenic 30.0%
! TCCTCATATCTTTTCTTCTT+CGG + chr4_4:44448527-44448546 None:intergenic 30.0%
! AATGATTTTGTGGAGATGAA+AGG - chr4_4:44448600-44448619 Msa0682620:intron 30.0%
AAAAGCTGCTTAGTTAGATT+TGG - chr4_4:44448695-44448714 Msa0682620:intron 30.0%
AACATTCTAGTAAGTTCAGA+CGG - chr4_4:44448836-44448855 Msa0682620:five_prime_UTR 30.0%
GAAAAAAGGATGGGAAATAA+AGG + chr4_4:44448248-44448267 None:intergenic 30.0%
TAGGTAAAGGAAAAAAGGAT+GGG + chr4_4:44448257-44448276 None:intergenic 30.0%
! TCCTCATATCTTTTCTTCTT+CGG + chr4_4:44448527-44448546 None:intergenic 30.0%
! AATGATTTTGTGGAGATGAA+AGG - chr4_4:44448600-44448619 Msa0682620:intron 30.0%
AAAAGCTGCTTAGTTAGATT+TGG - chr4_4:44448695-44448714 Msa0682620:intron 30.0%
AACATTCTAGTAAGTTCAGA+CGG - chr4_4:44448836-44448855 Msa0682620:five_prime_UTR 30.0%
AAAAGGATGGGAAATAAAGG+AGG + chr4_4:44448245-44448264 None:intergenic 35.0%
CTAGGTAAAGGAAAAAAGGA+TGG + chr4_4:44448258-44448277 None:intergenic 35.0%
!! GTTTAACATGGACTGCATTT+TGG + chr4_4:44448296-44448315 None:intergenic 35.0%
GTGAGATCATAGATCAAAAG+AGG + chr4_4:44448422-44448441 None:intergenic 35.0%
!! ATGTTTGAATTTGATGGTGC+AGG - chr4_4:44448487-44448506 Msa0682620:CDS 35.0%
ACCGAAGAAGAAAAGATATG+AGG - chr4_4:44448523-44448542 Msa0682620:CDS 35.0%
AGTGCATGCAAATCAAGAAA+AGG - chr4_4:44448557-44448576 Msa0682620:CDS 35.0%
GTGCATGCAAATCAAGAAAA+GGG - chr4_4:44448558-44448577 Msa0682620:CDS 35.0%
! TGATGATGATCTTGAAGATC+TGG - chr4_4:44448623-44448642 Msa0682620:intron 35.0%
AACTGATGTGACCAATGAAT+AGG - chr4_4:44448864-44448883 Msa0682620:five_prime_UTR 35.0%
ACTGATGTGACCAATGAATA+GGG - chr4_4:44448865-44448884 Msa0682620:five_prime_UTR 35.0%
TTCGTACGGACATATTTGTA+TGG - chr4_4:44448894-44448913 Msa0682620:five_prime_UTR 35.0%
AAAAGGATGGGAAATAAAGG+AGG + chr4_4:44448245-44448264 None:intergenic 35.0%
CTAGGTAAAGGAAAAAAGGA+TGG + chr4_4:44448258-44448277 None:intergenic 35.0%
!! GTTTAACATGGACTGCATTT+TGG + chr4_4:44448296-44448315 None:intergenic 35.0%
GTGAGATCATAGATCAAAAG+AGG + chr4_4:44448422-44448441 None:intergenic 35.0%
!! ATGTTTGAATTTGATGGTGC+AGG - chr4_4:44448487-44448506 Msa0682620:CDS 35.0%
ACCGAAGAAGAAAAGATATG+AGG - chr4_4:44448523-44448542 Msa0682620:CDS 35.0%
AGTGCATGCAAATCAAGAAA+AGG - chr4_4:44448557-44448576 Msa0682620:CDS 35.0%
GTGCATGCAAATCAAGAAAA+GGG - chr4_4:44448558-44448577 Msa0682620:CDS 35.0%
! TGATGATGATCTTGAAGATC+TGG - chr4_4:44448623-44448642 Msa0682620:intron 35.0%
AACTGATGTGACCAATGAAT+AGG - chr4_4:44448864-44448883 Msa0682620:five_prime_UTR 35.0%
ACTGATGTGACCAATGAATA+GGG - chr4_4:44448865-44448884 Msa0682620:five_prime_UTR 35.0%
TTCGTACGGACATATTTGTA+TGG - chr4_4:44448894-44448913 Msa0682620:five_prime_UTR 35.0%
AGTAGCACGAAGGTTTAACA+TGG + chr4_4:44448308-44448327 None:intergenic 40.0%
! TTAAACCTTCGTGCTACTCA+TGG - chr4_4:44448310-44448329 Msa0682620:three_prime_UTR 40.0%
! ACTCTTTGGAAAGAGCAAGA+GGG + chr4_4:44448366-44448385 None:intergenic 40.0%
! TACTCTTTGGAAAGAGCAAG+AGG + chr4_4:44448367-44448386 None:intergenic 40.0%
!! TTGAATTTGATGGTGCAGGA+TGG - chr4_4:44448491-44448510 Msa0682620:CDS 40.0%
CTGTCTCTTACTTATCTCAC+AGG - chr4_4:44448663-44448682 Msa0682620:intron 40.0%
CGAAGTTTCACCCTATTCAT+TGG + chr4_4:44448878-44448897 None:intergenic 40.0%
GAATAGGGTGAAACTTCGTA+CGG - chr4_4:44448880-44448899 Msa0682620:five_prime_UTR 40.0%
AGTAGCACGAAGGTTTAACA+TGG + chr4_4:44448308-44448327 None:intergenic 40.0%
! TTAAACCTTCGTGCTACTCA+TGG - chr4_4:44448310-44448329 Msa0682620:three_prime_UTR 40.0%
! ACTCTTTGGAAAGAGCAAGA+GGG + chr4_4:44448366-44448385 None:intergenic 40.0%
! TACTCTTTGGAAAGAGCAAG+AGG + chr4_4:44448367-44448386 None:intergenic 40.0%
!! TTGAATTTGATGGTGCAGGA+TGG - chr4_4:44448491-44448510 Msa0682620:CDS 40.0%
CTGTCTCTTACTTATCTCAC+AGG - chr4_4:44448663-44448682 Msa0682620:intron 40.0%
CGAAGTTTCACCCTATTCAT+TGG + chr4_4:44448878-44448897 None:intergenic 40.0%
GAATAGGGTGAAACTTCGTA+CGG - chr4_4:44448880-44448899 Msa0682620:five_prime_UTR 40.0%
GTGATTCCAGAAAGACAGAG+AGG + chr4_4:44448340-44448359 None:intergenic 45.0%
TTTGGAAAGAGCAAGAGGGT+TGG + chr4_4:44448362-44448381 None:intergenic 45.0%
GTGATTCCAGAAAGACAGAG+AGG + chr4_4:44448340-44448359 None:intergenic 45.0%
TTTGGAAAGAGCAAGAGGGT+TGG + chr4_4:44448362-44448381 None:intergenic 45.0%
GAACTCCATGAGTAGCACGA+AGG + chr4_4:44448318-44448337 None:intergenic 50.0%
GGAGTTCCTCTCTGTCTTTC+TGG - chr4_4:44448331-44448350 Msa0682620:three_prime_UTR 50.0%
GAACTCCATGAGTAGCACGA+AGG + chr4_4:44448318-44448337 None:intergenic 50.0%
GGAGTTCCTCTCTGTCTTTC+TGG - chr4_4:44448331-44448350 Msa0682620:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 44448168 44448956 44448168 ID=Msa0682620;Name=Msa0682620
chr4_4 mRNA 44448168 44448956 44448168 ID=Msa0682620-mRNA-1;Parent=Msa0682620;Name=Msa0682620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=130|1|1|1|1|1|2|249|94
chr4_4 exon 44448168 44448615 44448168 ID=Msa0682620-mRNA-1:exon:10739;Parent=Msa0682620-mRNA-1
chr4_4 exon 44448741 44448956 44448741 ID=Msa0682620-mRNA-1:exon:10738;Parent=Msa0682620-mRNA-1
chr4_4 five_prime_UTR 44448827 44448956 44448827 ID=Msa0682620-mRNA-1:five_prime_utr;Parent=Msa0682620-mRNA-1
chr4_4 CDS 44448741 44448826 44448741 ID=Msa0682620-mRNA-1:cds;Parent=Msa0682620-mRNA-1
chr4_4 CDS 44448417 44448615 44448417 ID=Msa0682620-mRNA-1:cds;Parent=Msa0682620-mRNA-1
chr4_4 three_prime_UTR 44448168 44448416 44448168 ID=Msa0682620-mRNA-1:three_prime_utr;Parent=Msa0682620-mRNA-1
Gene Sequence

>Msa0682620

ATGCAGTCCATGTTAAACCTTCGTGCTACTCATGGAGTTCCTCTCTGTCTTTCTGGAATCACCAACCCTCTTGCTCTTTCCAAAGAGATGGCAGATCACGACCGAAGAAGAAAAGATATGAGGAAGCAAAGATCAGTGCATGCAAATCAAGAAAAGGGTGCACAAAGTCAAAGAAACAATGATTTTGTGGAGATGAAAGGTGATGATGATCTTGAAGATCTGGATGAAATCTTGCATCAACTGTCTCTTACTTATCTCACAGGTTTATCAGCAAAAGCTGCTTAG

Protein sequence

>Msa0682620

MQSMLNLRATHGVPLCLSGITNPLALSKEMADHDRRRKDMRKQRSVHANQEKGAQSQRNNDFVEMKGDDDLEDLDEILHQLSLTYLTGLSAKAA*