AlfalfaGEDB | Msa1145540

M. sativa cultivar zhongmu-4 / Msa1145540

KEGG: -
GO: -
PFAM: PF05553.14

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1145540	XP_003590011.1	95.161	186	9	0	1	186	1	186	1.70e-119	346

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1145540	G7I4N6	95.161	186	9	0	1	186	1	186	8.13e-120	346

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0030160	Msa1145540	0.809611	1.737408e-50	-8.615850e-47
Msa0092410	Msa1145540	0.808528	2.963473e-50	-8.615850e-47
Msa0106710	Msa1145540	0.800665	1.295398e-48	-8.615850e-47
Msa0682070	Msa1145540	0.843339	1.488761e-58	-8.615850e-47
Msa0713510	Msa1145540	0.822970	1.781437e-53	-8.615850e-47
Msa0797250	Msa1145540	0.820325	7.282298e-53	-8.615850e-47
Msa0828240	Msa1145540	0.804569	2.028120e-49	-8.615850e-47
Msa0156300	Msa1145540	0.954418	3.093213e-112	-8.615850e-47
Msa0187830	Msa1145540	0.805570	1.252635e-49	-8.615850e-47
Msa0191890	Msa1145540	0.819723	9.999110e-53	-8.615850e-47
Msa0227340	Msa1145540	0.800355	1.498454e-48	-8.615850e-47
Msa0232020	Msa1145540	0.816875	4.414316e-52	-8.615850e-47
Msa0408340	Msa1145540	0.802687	4.984677e-49	-8.615850e-47
Msa0422180	Msa1145540	0.815261	1.012026e-51	-8.615850e-47
Msa0432160	Msa1145540	0.802427	5.638208e-49	-8.615850e-47
Msa0432670	Msa1145540	0.811594	6.476352e-51	-8.615850e-47
Msa0476680	Msa1145540	0.809314	2.012095e-50	-8.615850e-47
Msa0981040	Msa1145540	0.802333	5.895730e-49	-8.615850e-47
Msa1034500	Msa1145540	0.800941	1.137654e-48	-8.615850e-47
Msa1043120	Msa1145540	0.808677	2.754239e-50	-8.615850e-47
Msa0264830	Msa1145540	0.810612	1.057295e-50	-8.615850e-47
Msa0269560	Msa1145540	0.823978	1.035358e-53	-8.615850e-47
Msa0278450	Msa1145540	0.807042	6.132467e-50	-8.615850e-47
Msa0302870	Msa1145540	0.805943	1.045553e-49	-8.615850e-47
Msa0312530	Msa1145540	0.820231	7.651537e-53	-8.615850e-47
Msa0340340	Msa1145540	0.809218	2.109615e-50	-8.615850e-47
Msa1143020	Msa1145540	0.818916	1.527220e-52	-8.615850e-47
Msa1145540	Msa1219900	0.817666	2.930349e-52	-8.615850e-47
Msa1145540	Msa1266490	0.841161	5.620271e-58	-8.615850e-47
Msa1145540	Msa1345810	0.819272	1.267244e-52	-8.615850e-47
Msa1145540	Msa1349450	0.801957	7.044743e-49	-8.615850e-47
Msa1145540	Msa1365610	0.801283	9.685094e-49	-8.615850e-47
Msa1145540	Msa1401270	0.820537	6.511036e-53	-8.615850e-47
Msa1145540	Msa1409490	0.832883	7.330572e-56	-8.615850e-47
Msa1145540	Msa1424630	0.801084	1.063843e-48	-8.615850e-47
Msa1145540	Msa1430550	0.821930	3.106436e-53	-8.615850e-47
Msa1145540	Msa1441270	0.976685	2.663727e-142	-8.615850e-47
Msa0501410	Msa1145540	0.824513	7.749018e-54	-8.615850e-47
Msa0521010	Msa1145540	0.811579	6.523655e-51	-8.615850e-47
Msa0546520	Msa1145540	0.816458	5.472336e-52	-8.615850e-47
Msa0899970	Msa1145540	0.803896	2.800786e-49	-8.615850e-47
Msa0902830	Msa1145540	0.804028	2.629060e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1145540	MtrunA17_Chr1g0167501	95.161	186	9	0	1	186	105	290	2.63e-121	344
Msa1145540	MtrunA17_Chr1g0187721	41.176	187	64	7	1	186	1	142	5.01e-33	115
Msa1145540	MtrunA17_Chr7g0261301	29.016	193	114	7	6	184	4	187	5.60e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1145540	AT4G14380.1	30.189	212	110	5	1	186	1	200	3.36e-26	99.8

Find 19 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTTCTTCCATGCTTTCT+TGG	0.227721	7_4:-22353471	None:intergenic
ATAAATTTCGTGAAGATATA+AGG	0.252185	7_4:+22353915	Msa1145540:CDS
TTCTTCCATGCTTTCTTGGC+TGG	0.283893	7_4:-22353467	None:intergenic
CATGGCAAGTAGTGGTTTCT+AGG	0.324426	7_4:+22353849	Msa1145540:CDS
CAATGATGTAGATACTTTAG+AGG	0.423826	7_4:+22353823	Msa1145540:CDS
TCTCTATTCTCAAACTCATT+CGG	0.450268	7_4:+22353712	Msa1145540:CDS
AATGTTGCAAGTGGATCAAA+AGG	0.493765	7_4:+22353877	Msa1145540:CDS
TGAGGTTATGAGATGAGTGA+GGG	0.528444	7_4:-22353582	None:intergenic
TTGAGGTTATGAGATGAGTG+AGG	0.532018	7_4:-22353583	None:intergenic
TGTTGAAAATGGTAATAAGA+AGG	0.558592	7_4:-22353659	None:intergenic
TTAAACCAGCCAAGAAAGCA+TGG	0.562063	7_4:+22353462	Msa1145540:CDS
TAGGTCTCCAATGTTGCAAG+TGG	0.566515	7_4:+22353868	Msa1145540:CDS
TAGATACTTTAGAGGATGCA+TGG	0.571579	7_4:+22353831	Msa1145540:CDS
AACAAGTAGAGAAATTGAAA+AGG	0.571929	7_4:+22353781	Msa1145540:CDS
AGAGTGAAAGAATGTGTTGA+AGG	0.572500	7_4:-22353553	None:intergenic
AGAGGATGCATGGCAAGTAG+TGG	0.577407	7_4:+22353841	Msa1145540:CDS
CATTGCTTGAATTTCATGAG+AGG	0.602579	7_4:+22353954	Msa1145540:CDS
GAGTGAAAGAATGTGTTGAA+GGG	0.655279	7_4:-22353552	None:intergenic
AGTAATATTCATCATGCACA+AGG	0.662096	7_4:+22353755	Msa1145540:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTGATATGTTTAGTTTG+TGG	-	chr7_4:22353520-22353539	None:intergenic	20.0%
!!	ATAAATTTCGTGAAGATATA+AGG	+	chr7_4:22353915-22353934	Msa1145540:CDS	20.0%
!!!	TTAGTTTGTGGAATTTTGAT+TGG	-	chr7_4:22353508-22353527	None:intergenic	25.0%
!	TGTTGAAAATGGTAATAAGA+AGG	-	chr7_4:22353662-22353681	None:intergenic	25.0%
!	AACAAGTAGAGAAATTGAAA+AGG	+	chr7_4:22353781-22353800	Msa1145540:CDS	25.0%
!!!	ACGATGAGTTTTACTTTTTG+AGG	-	chr7_4:22353603-22353622	None:intergenic	30.0%
!	CAAAGCTTTTGTGTTGAAAA+TGG	-	chr7_4:22353673-22353692	None:intergenic	30.0%
	TCTCTATTCTCAAACTCATT+CGG	+	chr7_4:22353712-22353731	Msa1145540:CDS	30.0%
	AGTAATATTCATCATGCACA+AGG	+	chr7_4:22353755-22353774	Msa1145540:CDS	30.0%
	CAATGATGTAGATACTTTAG+AGG	+	chr7_4:22353823-22353842	Msa1145540:CDS	30.0%
	GAGTGAAAGAATGTGTTGAA+GGG	-	chr7_4:22353555-22353574	None:intergenic	35.0%
	AGAGTGAAAGAATGTGTTGA+AGG	-	chr7_4:22353556-22353575	None:intergenic	35.0%
!	TAGATACTTTAGAGGATGCA+TGG	+	chr7_4:22353831-22353850	Msa1145540:CDS	35.0%
!	TTTTGATCCACTTGCAACAT+TGG	-	chr7_4:22353878-22353897	None:intergenic	35.0%
	AATGTTGCAAGTGGATCAAA+AGG	+	chr7_4:22353877-22353896	Msa1145540:CDS	35.0%
	CATTGCTTGAATTTCATGAG+AGG	+	chr7_4:22353954-22353973	Msa1145540:CDS	35.0%
	TTAAACCAGCCAAGAAAGCA+TGG	+	chr7_4:22353462-22353481	Msa1145540:CDS	40.0%
	GAAGTTCTTCCATGCTTTCT+TGG	-	chr7_4:22353474-22353493	None:intergenic	40.0%
	TGAGGTTATGAGATGAGTGA+GGG	-	chr7_4:22353585-22353604	None:intergenic	40.0%
	TTGAGGTTATGAGATGAGTG+AGG	-	chr7_4:22353586-22353605	None:intergenic	40.0%
	TTCTTCCATGCTTTCTTGGC+TGG	-	chr7_4:22353470-22353489	None:intergenic	45.0%
	CATGGCAAGTAGTGGTTTCT+AGG	+	chr7_4:22353849-22353868	Msa1145540:CDS	45.0%
	TAGGTCTCCAATGTTGCAAG+TGG	+	chr7_4:22353868-22353887	Msa1145540:CDS	45.0%
	AGAGGATGCATGGCAAGTAG+TGG	+	chr7_4:22353841-22353860	Msa1145540:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	22353434	22353994	22353434	ID=Msa1145540;Name=Msa1145540
chr7_4	mRNA	22353434	22353994	22353434	ID=Msa1145540-mRNA-1;Parent=Msa1145540;Name=Msa1145540-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|186
chr7_4	exon	22353434	22353994	22353434	ID=Msa1145540-mRNA-1:exon:5534;Parent=Msa1145540-mRNA-1
chr7_4	CDS	22353434	22353994	22353434	ID=Msa1145540-mRNA-1:cds;Parent=Msa1145540-mRNA-1

Gene Sequence

>Msa1145540

ATGTCACGCATGAAGTTATCAAAGAAACTTAAACCAGCCAAGAAAGCATGGAAGAACTTCTCAAACAATTTCCAATCAAAATTCCACAAACTAAACATATCAAAAACTTTCAAAACAACCCTTCAACACATTCTTTCACTCTTTCATTCCCTCACTCATCTCATAACCTCAAAAAGTAAAACTCATCGTTCTCTCACTTCTTTTAAATCATCATATGCTGCTTCACCTTCTTATTACCATTTTCAACACAAAAGCTTTGCAGCTATAAATATAAATGATCTCTATTCTCAAACTCATTCGGTTTCTTCTACGAATAATAGCAGTAATATTCATCATGCACAAGGTGAAACAAGTAGAGAAATTGAAAAGGTACATGTTGATGACAACAACAATGATGTAGATACTTTAGAGGATGCATGGCAAGTAGTGGTTTCTAGGTCTCCAATGTTGCAAGTGGATCAAAAGGCTGAAGAATTTATCTATAAATTTCGTGAAGATATAAGGCTTCAGAAAGAGAAATCATTGCTTGAATTTCATGAGAGGCTAGCAAGAAGTACCTAA

Protein sequence

>Msa1145540

MSRMKLSKKLKPAKKAWKNFSNNFQSKFHKLNISKTFKTTLQHILSLFHSLTHLITSKSKTHRSLTSFKSSYAASPSYYHFQHKSFAAININDLYSQTHSVSSTNNSSNIHHAQGETSREIEKVHVDDNNNDVDTLEDAWQVVVSRSPMLQVDQKAEEFIYKFREDIRLQKEKSLLEFHERLARST*