Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1441270 | G7I4N6 | 95.161 | 186 | 9 | 0 | 1 | 186 | 1 | 186 | 8.13e-120 | 346 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015670 | Msa1441270 | 0.811735 | 6.032885e-51 | -8.615850e-47 |
| Msa0030160 | Msa1441270 | 0.813844 | 2.083815e-51 | -8.615850e-47 |
| Msa0051050 | Msa1441270 | 0.807933 | 3.967964e-50 | -8.615850e-47 |
| Msa0092410 | Msa1441270 | 0.815761 | 7.833806e-52 | -8.615850e-47 |
| Msa0101240 | Msa1441270 | 0.811763 | 5.948865e-51 | -8.615850e-47 |
| Msa0106710 | Msa1441270 | 0.802172 | 6.362726e-49 | -8.615850e-47 |
| Msa1345810 | Msa1441270 | 0.813174 | 2.925394e-51 | -8.615850e-47 |
| Msa1349450 | Msa1441270 | 0.809070 | 2.268684e-50 | -8.615850e-47 |
| Msa1360420 | Msa1441270 | 0.814175 | 1.761309e-51 | -8.615850e-47 |
| Msa1401270 | Msa1441270 | 0.812664 | 3.782651e-51 | -8.615850e-47 |
| Msa1409490 | Msa1441270 | 0.845812 | 3.215355e-59 | -8.615850e-47 |
| Msa1424630 | Msa1441270 | 0.816338 | 5.821379e-52 | -8.615850e-47 |
| Msa1430550 | Msa1441270 | 0.833664 | 4.684250e-56 | -8.615850e-47 |
| Msa1441270 | Msa1463030 | 0.804903 | 1.727335e-49 | -8.615850e-47 |
| Msa0614380 | Msa1441270 | 0.807576 | 4.724859e-50 | -8.615850e-47 |
| Msa0682070 | Msa1441270 | 0.846682 | 1.862808e-59 | -8.615850e-47 |
| Msa0697590 | Msa1441270 | 0.815084 | 1.108118e-51 | -8.615850e-47 |
| Msa0708310 | Msa1441270 | 0.801933 | 7.126339e-49 | -8.615850e-47 |
| Msa0713510 | Msa1441270 | 0.814912 | 1.209572e-51 | -8.615850e-47 |
| Msa0797250 | Msa1441270 | 0.806883 | 6.626613e-50 | -8.615850e-47 |
| Msa0156300 | Msa1441270 | 0.960210 | 2.666268e-118 | -8.615850e-47 |
| Msa0187830 | Msa1441270 | 0.813763 | 2.171429e-51 | -8.615850e-47 |
| Msa0189710 | Msa1441270 | 0.802480 | 5.499168e-49 | -8.615850e-47 |
| Msa0191890 | Msa1441270 | 0.813759 | 2.175598e-51 | -8.615850e-47 |
| Msa0232020 | Msa1441270 | 0.826993 | 1.999527e-54 | -8.615850e-47 |
| Msa0232090 | Msa1441270 | 0.809221 | 2.105842e-50 | -8.615850e-47 |
| Msa1236440 | Msa1441270 | 0.805116 | 1.558897e-49 | -8.615850e-47 |
| Msa1266490 | Msa1441270 | 0.849071 | 4.090046e-60 | -8.615850e-47 |
| Msa0408340 | Msa1441270 | 0.810970 | 8.842640e-51 | -8.615850e-47 |
| Msa0422180 | Msa1441270 | 0.811031 | 8.581078e-51 | -8.615850e-47 |
| Msa0432670 | Msa1441270 | 0.807449 | 5.026575e-50 | -8.615850e-47 |
| Msa0476680 | Msa1441270 | 0.806154 | 9.441317e-50 | -8.615850e-47 |
| Msa1043120 | Msa1441270 | 0.817039 | 4.053711e-52 | -8.615850e-47 |
| Msa0240270 | Msa1441270 | 0.803538 | 3.323313e-49 | -8.615850e-47 |
| Msa0264830 | Msa1441270 | 0.808602 | 2.856755e-50 | -8.615850e-47 |
| Msa0269560 | Msa1441270 | 0.826927 | 2.073012e-54 | -8.615850e-47 |
| Msa0278450 | Msa1441270 | 0.805709 | 1.171131e-49 | -8.615850e-47 |
| Msa0283500 | Msa1441270 | 0.802344 | 5.866098e-49 | -8.615850e-47 |
| Msa0310090 | Msa1441270 | 0.804950 | 1.688985e-49 | -8.615850e-47 |
| Msa0312530 | Msa1441270 | 0.815466 | 9.112177e-52 | -8.615850e-47 |
| Msa0340340 | Msa1441270 | 0.802992 | 4.311665e-49 | -8.615850e-47 |
| Msa1143020 | Msa1441270 | 0.815777 | 7.768175e-52 | -8.615850e-47 |
| Msa1145540 | Msa1441270 | 0.976685 | 2.663727e-142 | -8.615850e-47 |
| Msa1161810 | Msa1441270 | 0.807325 | 5.340843e-50 | -8.615850e-47 |
| Msa1162060 | Msa1441270 | 0.807426 | 5.084215e-50 | -8.615850e-47 |
| Msa1214170 | Msa1441270 | 0.801066 | 1.073162e-48 | -8.615850e-47 |
| Msa1219900 | Msa1441270 | 0.829153 | 6.037473e-55 | -8.615850e-47 |
| Msa0501410 | Msa1441270 | 0.827121 | 1.863345e-54 | -8.615850e-47 |
| Msa0521010 | Msa1441270 | 0.809654 | 1.700646e-50 | -8.615850e-47 |
| Msa0521670 | Msa1441270 | 0.810488 | 1.124115e-50 | -8.615850e-47 |
| Msa0546520 | Msa1441270 | 0.816466 | 5.451661e-52 | -8.615850e-47 |
| Msa0579660 | Msa1441270 | 0.803277 | 3.763974e-49 | -8.615850e-47 |
| Msa0899970 | Msa1441270 | 0.803529 | 3.337346e-49 | -8.615850e-47 |
| Msa0902830 | Msa1441270 | 0.801309 | 9.569404e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1441270 | MtrunA17_Chr1g0167501 | 95.161 | 186 | 9 | 0 | 1 | 186 | 105 | 290 | 2.63e-121 | 344 |
| Msa1441270 | MtrunA17_Chr1g0187721 | 41.176 | 187 | 64 | 7 | 1 | 186 | 1 | 142 | 5.01e-33 | 115 |
| Msa1441270 | MtrunA17_Chr7g0261301 | 29.016 | 193 | 114 | 7 | 6 | 184 | 4 | 187 | 5.60e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1441270 | AT4G14380.1 | 30.189 | 212 | 110 | 5 | 1 | 186 | 1 | 200 | 3.36e-26 | 99.8 |
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTTCTTCCATGCTTTCT+TGG | 0.227721 | tig0029241:+30192 | Msa1441270:intergenic |
| ATAAATTTCGTGAAGATATA+AGG | 0.252185 | tig0029241:-29748 | Msa1441270:CDS |
| TTCTTCCATGCTTTCTTGGC+TGG | 0.283893 | tig0029241:+30196 | Msa1441270:intergenic |
| CATGGCAAGTAGTGGTTTCT+AGG | 0.324426 | tig0029241:-29814 | Msa1441270:CDS |
| CAATGATGTAGATACTTTAG+AGG | 0.423826 | tig0029241:-29840 | Msa1441270:CDS |
| TCTCTATTCTCAAACTCATT+CGG | 0.450268 | tig0029241:-29951 | Msa1441270:CDS |
| AATGTTGCAAGTGGATCAAA+AGG | 0.493765 | tig0029241:-29786 | Msa1441270:CDS |
| TGAGGTTATGAGATGAGTGA+GGG | 0.528444 | tig0029241:+30081 | Msa1441270:intergenic |
| TTGAGGTTATGAGATGAGTG+AGG | 0.532018 | tig0029241:+30080 | Msa1441270:intergenic |
| TGTTGAAAATGGTAATAAGA+AGG | 0.558592 | tig0029241:+30004 | Msa1441270:intergenic |
| TTAAACCAGCCAAGAAAGCA+TGG | 0.562063 | tig0029241:-30201 | Msa1441270:CDS |
| TAGGTCTCCAATGTTGCAAG+TGG | 0.566515 | tig0029241:-29795 | Msa1441270:CDS |
| TAGATACTTTAGAGGATGCA+TGG | 0.571579 | tig0029241:-29832 | Msa1441270:CDS |
| AACAAGTAGAGAAATTGAAA+AGG | 0.571929 | tig0029241:-29882 | Msa1441270:CDS |
| AGAGTGAAAGAATGTGTTGA+AGG | 0.572500 | tig0029241:+30110 | Msa1441270:intergenic |
| AGAGGATGCATGGCAAGTAG+TGG | 0.577407 | tig0029241:-29822 | Msa1441270:CDS |
| CATTGCTTGAATTTCATGAG+AGG | 0.602579 | tig0029241:-29709 | Msa1441270:CDS |
| GAGTGAAAGAATGTGTTGAA+GGG | 0.655279 | tig0029241:+30111 | Msa1441270:intergenic |
| AGTAATATTCATCATGCACA+AGG | 0.662096 | tig0029241:-29908 | Msa1441270:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTGATATGTTTAGTTTG+TGG | + | tig0029241:29777-29796 | Msa1441270:intergenic | 20.0% |
| !! | ATAAATTTCGTGAAGATATA+AGG | - | tig0029241:30172-30191 | Msa1441270:CDS | 20.0% |
| !!! | TTAGTTTGTGGAATTTTGAT+TGG | + | tig0029241:29765-29784 | Msa1441270:intergenic | 25.0% |
| ! | TGTTGAAAATGGTAATAAGA+AGG | + | tig0029241:29919-29938 | Msa1441270:intergenic | 25.0% |
| ! | AACAAGTAGAGAAATTGAAA+AGG | - | tig0029241:30038-30057 | Msa1441270:CDS | 25.0% |
| !!! | ACGATGAGTTTTACTTTTTG+AGG | + | tig0029241:29860-29879 | Msa1441270:intergenic | 30.0% |
| ! | CAAAGCTTTTGTGTTGAAAA+TGG | + | tig0029241:29930-29949 | Msa1441270:intergenic | 30.0% |
| TCTCTATTCTCAAACTCATT+CGG | - | tig0029241:29969-29988 | Msa1441270:CDS | 30.0% | |
| AGTAATATTCATCATGCACA+AGG | - | tig0029241:30012-30031 | Msa1441270:CDS | 30.0% | |
| CAATGATGTAGATACTTTAG+AGG | - | tig0029241:30080-30099 | Msa1441270:CDS | 30.0% | |
| GAGTGAAAGAATGTGTTGAA+GGG | + | tig0029241:29812-29831 | Msa1441270:intergenic | 35.0% | |
| AGAGTGAAAGAATGTGTTGA+AGG | + | tig0029241:29813-29832 | Msa1441270:intergenic | 35.0% | |
| ! | TAGATACTTTAGAGGATGCA+TGG | - | tig0029241:30088-30107 | Msa1441270:CDS | 35.0% |
| ! | TTTTGATCCACTTGCAACAT+TGG | + | tig0029241:30135-30154 | Msa1441270:intergenic | 35.0% |
| AATGTTGCAAGTGGATCAAA+AGG | - | tig0029241:30134-30153 | Msa1441270:CDS | 35.0% | |
| CATTGCTTGAATTTCATGAG+AGG | - | tig0029241:30211-30230 | Msa1441270:CDS | 35.0% | |
| TTAAACCAGCCAAGAAAGCA+TGG | - | tig0029241:29719-29738 | Msa1441270:CDS | 40.0% | |
| GAAGTTCTTCCATGCTTTCT+TGG | + | tig0029241:29731-29750 | Msa1441270:intergenic | 40.0% | |
| TGAGGTTATGAGATGAGTGA+GGG | + | tig0029241:29842-29861 | Msa1441270:intergenic | 40.0% | |
| TTGAGGTTATGAGATGAGTG+AGG | + | tig0029241:29843-29862 | Msa1441270:intergenic | 40.0% | |
| TTCTTCCATGCTTTCTTGGC+TGG | + | tig0029241:29727-29746 | Msa1441270:intergenic | 45.0% | |
| CATGGCAAGTAGTGGTTTCT+AGG | - | tig0029241:30106-30125 | Msa1441270:CDS | 45.0% | |
| TAGGTCTCCAATGTTGCAAG+TGG | - | tig0029241:30125-30144 | Msa1441270:CDS | 45.0% | |
| AGAGGATGCATGGCAAGTAG+TGG | - | tig0029241:30098-30117 | Msa1441270:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0029241 | gene | 29691 | 30251 | 29691 | ID=Msa1441270;Name=Msa1441270 |
| tig0029241 | mRNA | 29691 | 30251 | 29691 | ID=Msa1441270-mRNA-1;Parent=Msa1441270;Name=Msa1441270-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|186 |
| tig0029241 | exon | 29691 | 30251 | 29691 | ID=Msa1441270-mRNA-1:exon:12728;Parent=Msa1441270-mRNA-1 |
| tig0029241 | CDS | 29691 | 30251 | 29691 | ID=Msa1441270-mRNA-1:cds;Parent=Msa1441270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
