AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1227220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1227220 MtrunA17_Chr1g0155831 90.000 200 14 3 1 199 1 195 5.84e-93 269
Msa1227220 MtrunA17_Chr3g0142231 54.930 213 75 8 1 198 1 207 9.76e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGGTGGATGGGGATG+GGG 0.237687 8_2:-2313305 Msa1227220:CDS
GAGGAAGTGGAGGATTTAAA+TGG 0.238474 8_2:-2313388 Msa1227220:CDS
GGAGGAGGTGGTGGTGGTGG+TGG 0.254756 8_2:-2313329 Msa1227220:CDS
GGTGGATGGGGATGGGGAGG+AGG 0.258829 8_2:-2313299 Msa1227220:CDS
TGGGGTGGAGGAGGTGGAGG+TGG 0.259357 8_2:-2313362 Msa1227220:CDS
GGTGGAGGAGGAGGTGGTGG+TGG 0.263820 8_2:-2313335 Msa1227220:CDS
GGAGGAGGAGGTGGTGGTGG+TGG 0.265833 8_2:-2313332 Msa1227220:CDS
GGTGATGGTGGAGGAGGAGG+TGG 0.275200 8_2:-2313341 Msa1227220:CDS
TGGTGGTGGTGGATGGGGAT+GGG 0.277073 8_2:-2313306 Msa1227220:CDS
TGGGGAGGAGGAGGTGGAGG+GGG 0.277304 8_2:-2313287 Msa1227220:CDS
GGATGGGGTGGAGGAGGTGG+AGG 0.281619 8_2:-2313365 Msa1227220:CDS
GATGGTGGAGGAGGAGGTGG+TGG 0.286277 8_2:-2313338 Msa1227220:CDS
GGATGGGGATGGGGAGGAGG+AGG 0.287599 8_2:-2313296 Msa1227220:CDS
TGGGGATGGGGAGGAGGAGG+TGG 0.287796 8_2:-2313293 Msa1227220:CDS
GGTGGTGATGGTGGAGGAGG+AGG 0.299047 8_2:-2313344 Msa1227220:CDS
TGGGGATGGGGTGGAGGAGG+TGG 0.300827 8_2:-2313368 Msa1227220:CDS
GGAGGTGGAGGTGGTGATGG+TGG 0.306423 8_2:-2313353 Msa1227220:CDS
GGATGGGGAGGAGGAGGTGG+AGG 0.309350 8_2:-2313290 Msa1227220:CDS
GTGGTGGTGGTGGATGGGGA+TGG 0.311711 8_2:-2313307 Msa1227220:CDS
TTCCCTTTAACATGTGATCT+TGG 0.313769 8_2:+2313233 None:intergenic
GGGAAGAGAGGAACTATAAA+AGG 0.318030 8_2:-2313215 Msa1227220:CDS
GGGGTGGTTGGTGGAAATGG+GGG 0.329480 8_2:-2313268 Msa1227220:CDS
GGAGGAGGTGGAGGTGGTGA+TGG 0.336564 8_2:-2313356 Msa1227220:CDS
GGTGGTGGATGGGGATGGGG+AGG 0.340139 8_2:-2313302 Msa1227220:CDS
GGAGGTGGTGATGGTGGAGG+AGG 0.340807 8_2:-2313347 Msa1227220:CDS
ATGGGGAGGAGGAGGTGGAG+GGG 0.347064 8_2:-2313288 Msa1227220:CDS
GAACCAAGATCACATGTTAA+AGG 0.355600 8_2:-2313236 Msa1227220:CDS
TTTAAATGGGGATGGGGTGG+AGG 0.374335 8_2:-2313374 Msa1227220:CDS
CTCTAATCATGAGGCTAGTA+AGG 0.377011 8_2:-2313480 Msa1227220:CDS
GGTGGAGGTGGTGATGGTGG+AGG 0.380555 8_2:-2313350 Msa1227220:CDS
AAATGGGGATGGGGTGGAGG+AGG 0.395075 8_2:-2313371 Msa1227220:CDS
GACAATGAGCTTTGCACATA+TGG 0.410730 8_2:+2313042 None:intergenic
GGATTTAAATGGGGATGGGG+TGG 0.411725 8_2:-2313377 Msa1227220:CDS
CCAAGGAAGAATATAGGTTA+GGG 0.426255 8_2:-2313157 Msa1227220:CDS
AGGAAGTGGAGGATTTAAAT+GGG 0.430452 8_2:-2313387 Msa1227220:CDS
GATGGGGAGGAGGAGGTGGA+GGG 0.441480 8_2:-2313289 Msa1227220:CDS
CCCTAACCTATATTCTTCCT+TGG 0.445933 8_2:+2313157 None:intergenic
TCCAAGGAAGAATATAGGTT+AGG 0.451383 8_2:-2313158 Msa1227220:CDS
ATGCATCCAAGGAAGAATAT+AGG 0.459436 8_2:-2313163 Msa1227220:CDS
AACCAAGATCACATGTTAAA+GGG 0.461053 8_2:-2313235 Msa1227220:CDS
TGGTATAAGAGAGGAGGAAG+TGG 0.473761 8_2:-2313401 Msa1227220:CDS
GGAGGATTTAAATGGGGATG+GGG 0.477845 8_2:-2313380 Msa1227220:CDS
TATAAGAGAGGAGGAAGTGG+AGG 0.499057 8_2:-2313398 Msa1227220:CDS
GAACAAGGTGCAAAGGTATG+AGG 0.513704 8_2:-2313112 Msa1227220:CDS
TGGAGGATTTAAATGGGGAT+GGG 0.517095 8_2:-2313381 Msa1227220:CDS
GTGGAGGATTTAAATGGGGA+TGG 0.535106 8_2:-2313382 Msa1227220:CDS
GCTAGTAAGGAACATGTAAG+AGG 0.540894 8_2:-2313467 Msa1227220:CDS
ATTCTGACAGTGGAAATTGT+TGG 0.544581 8_2:-2313569 Msa1227220:CDS
TCAACACAAGCAAAGGTAGC+TGG 0.560749 8_2:-2313421 Msa1227220:CDS
ACAACATCAACATGCATCCA+AGG 0.561027 8_2:-2313174 Msa1227220:CDS
AAGGTCCACCACAGTGAAGA+GGG 0.578681 8_2:+2313081 None:intergenic
TACTAGCCTCATGATTAGAG+AGG 0.594661 8_2:+2313483 None:intergenic
AAGGTGCAAAGGTATGAGGT+TGG 0.596084 8_2:-2313108 Msa1227220:CDS
GATTGCCCTCTTCACTGTGG+TGG 0.599999 8_2:-2313086 Msa1227220:CDS
ACTAGCCTCATGATTAGAGA+GGG 0.605706 8_2:+2313484 None:intergenic
GGTAGCTGGTATAAGAGAGG+AGG 0.607987 8_2:-2313407 Msa1227220:CDS
CAAGGTCCACCACAGTGAAG+AGG 0.609797 8_2:+2313080 None:intergenic
CACAATGCATGACAAGAACA+AGG 0.612043 8_2:-2313127 Msa1227220:CDS
CTGCTTGATAATTCTGACAG+TGG 0.615962 8_2:-2313579 Msa1227220:CDS
TCACATGTTAAAGGGAAGAG+AGG 0.617086 8_2:-2313227 Msa1227220:CDS
AATGACACTGCTCAAAGTCA+AGG 0.622542 8_2:-2313524 Msa1227220:CDS
GTTGTGTTCAACACAAGCAA+AGG 0.625062 8_2:-2313428 Msa1227220:CDS
ATGACAAGAACAAGGTGCAA+AGG 0.633795 8_2:-2313119 Msa1227220:CDS
AGCAACCCTCTCTAATCATG+AGG 0.635131 8_2:-2313489 Msa1227220:CDS
AAAGGTAGCTGGTATAAGAG+AGG 0.636029 8_2:-2313410 Msa1227220:CDS
TTGGATTGCCCTCTTCACTG+TGG 0.665531 8_2:-2313089 Msa1227220:CDS
TGGTAGCAGTCATAGAAACA+AGG 0.674985 8_2:+2313062 None:intergenic
GGAAGTGGAGGATTTAAATG+GGG 0.675622 8_2:-2313386 Msa1227220:CDS
CAAGGAAGAATATAGGTTAG+GGG 0.686210 8_2:-2313156 Msa1227220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACCAAGATCACATGTTAAA+GGG - chr8_2:2313404-2313423 Msa1227220:CDS 30.0%
ATTCTGACAGTGGAAATTGT+TGG - chr8_2:2313070-2313089 Msa1227220:CDS 35.0%
AGGAAGTGGAGGATTTAAAT+GGG - chr8_2:2313252-2313271 Msa1227220:CDS 35.0%
GAACCAAGATCACATGTTAA+AGG - chr8_2:2313403-2313422 Msa1227220:CDS 35.0%
TTCCCTTTAACATGTGATCT+TGG + chr8_2:2313409-2313428 None:intergenic 35.0%
ATGCATCCAAGGAAGAATAT+AGG - chr8_2:2313476-2313495 Msa1227220:CDS 35.0%
TCCAAGGAAGAATATAGGTT+AGG - chr8_2:2313481-2313500 Msa1227220:CDS 35.0%
CCAAGGAAGAATATAGGTTA+GGG - chr8_2:2313482-2313501 Msa1227220:CDS 35.0%
CAAGGAAGAATATAGGTTAG+GGG - chr8_2:2313483-2313502 Msa1227220:CDS 35.0%
! CTGCTTGATAATTCTGACAG+TGG - chr8_2:2313060-2313079 Msa1227220:CDS 40.0%
AATGACACTGCTCAAAGTCA+AGG - chr8_2:2313115-2313134 Msa1227220:CDS 40.0%
! ACTAGCCTCATGATTAGAGA+GGG + chr8_2:2313158-2313177 None:intergenic 40.0%
! TACTAGCCTCATGATTAGAG+AGG + chr8_2:2313159-2313178 None:intergenic 40.0%
CTCTAATCATGAGGCTAGTA+AGG - chr8_2:2313159-2313178 Msa1227220:CDS 40.0%
GCTAGTAAGGAACATGTAAG+AGG - chr8_2:2313172-2313191 Msa1227220:CDS 40.0%
GTTGTGTTCAACACAAGCAA+AGG - chr8_2:2313211-2313230 Msa1227220:CDS 40.0%
AAAGGTAGCTGGTATAAGAG+AGG - chr8_2:2313229-2313248 Msa1227220:CDS 40.0%
GAGGAAGTGGAGGATTTAAA+TGG - chr8_2:2313251-2313270 Msa1227220:CDS 40.0%
GGAAGTGGAGGATTTAAATG+GGG - chr8_2:2313253-2313272 Msa1227220:CDS 40.0%
TGGAGGATTTAAATGGGGAT+GGG - chr8_2:2313258-2313277 Msa1227220:CDS 40.0%
TCACATGTTAAAGGGAAGAG+AGG - chr8_2:2313412-2313431 Msa1227220:CDS 40.0%
GGGAAGAGAGGAACTATAAA+AGG - chr8_2:2313424-2313443 Msa1227220:CDS 40.0%
ACAACATCAACATGCATCCA+AGG - chr8_2:2313465-2313484 Msa1227220:CDS 40.0%
CCCTAACCTATATTCTTCCT+TGG + chr8_2:2313485-2313504 None:intergenic 40.0%
CACAATGCATGACAAGAACA+AGG - chr8_2:2313512-2313531 Msa1227220:CDS 40.0%
ATGACAAGAACAAGGTGCAA+AGG - chr8_2:2313520-2313539 Msa1227220:CDS 40.0%
TGGTAGCAGTCATAGAAACA+AGG + chr8_2:2313580-2313599 None:intergenic 40.0%
GACAATGAGCTTTGCACATA+TGG + chr8_2:2313600-2313619 None:intergenic 40.0%
AGCAACCCTCTCTAATCATG+AGG - chr8_2:2313150-2313169 Msa1227220:CDS 45.0%
TCAACACAAGCAAAGGTAGC+TGG - chr8_2:2313218-2313237 Msa1227220:CDS 45.0%
TGGTATAAGAGAGGAGGAAG+TGG - chr8_2:2313238-2313257 Msa1227220:CDS 45.0%
TATAAGAGAGGAGGAAGTGG+AGG - chr8_2:2313241-2313260 Msa1227220:CDS 45.0%
GTGGAGGATTTAAATGGGGA+TGG - chr8_2:2313257-2313276 Msa1227220:CDS 45.0%
GGAGGATTTAAATGGGGATG+GGG - chr8_2:2313259-2313278 Msa1227220:CDS 45.0%
! GAACAAGGTGCAAAGGTATG+AGG - chr8_2:2313527-2313546 Msa1227220:CDS 45.0%
! AAGGTGCAAAGGTATGAGGT+TGG - chr8_2:2313531-2313550 Msa1227220:CDS 45.0%
GGTAGCTGGTATAAGAGAGG+AGG - chr8_2:2313232-2313251 Msa1227220:CDS 50.0%
GGATTTAAATGGGGATGGGG+TGG - chr8_2:2313262-2313281 Msa1227220:CDS 50.0%
TTTAAATGGGGATGGGGTGG+AGG - chr8_2:2313265-2313284 Msa1227220:CDS 50.0%
TTGGATTGCCCTCTTCACTG+TGG - chr8_2:2313550-2313569 Msa1227220:CDS 50.0%
AAGGTCCACCACAGTGAAGA+GGG + chr8_2:2313561-2313580 None:intergenic 50.0%
!! AGGGGGTGGTTGGTGGAAAT+GGG - chr8_2:2313369-2313388 Msa1227220:CDS 55.0%
! TGGTGGAAATGGGGGTGTAG+AGG - chr8_2:2313379-2313398 Msa1227220:CDS 55.0%
GATTGCCCTCTTCACTGTGG+TGG - chr8_2:2313553-2313572 Msa1227220:CDS 55.0%
CAAGGTCCACCACAGTGAAG+AGG + chr8_2:2313562-2313581 None:intergenic 55.0%
AAATGGGGATGGGGTGGAGG+AGG - chr8_2:2313268-2313287 Msa1227220:CDS 60.0%
!! TGGTGGTGGTGGTGGTGGAT+GGG - chr8_2:2313327-2313346 Msa1227220:CDS 60.0%
!! TGGTGGTGGTGGATGGGGAT+GGG - chr8_2:2313333-2313352 Msa1227220:CDS 60.0%
!! GAGGGGGTGGTTGGTGGAAA+TGG - chr8_2:2313368-2313387 Msa1227220:CDS 60.0%
!! GGGGGTGGTTGGTGGAAATG+GGG - chr8_2:2313370-2313389 Msa1227220:CDS 60.0%
!! GGGGTGGTTGGTGGAAATGG+GGG - chr8_2:2313371-2313390 Msa1227220:CDS 60.0%
GGAGGAGGTGGAGGTGGTGA+TGG - chr8_2:2313283-2313302 Msa1227220:CDS 65.0%
!! GGAGGTGGAGGTGGTGATGG+TGG - chr8_2:2313286-2313305 Msa1227220:CDS 65.0%
!! GGTGGAGGTGGTGATGGTGG+AGG - chr8_2:2313289-2313308 Msa1227220:CDS 65.0%
!! GGAGGTGGTGATGGTGGAGG+AGG - chr8_2:2313292-2313311 Msa1227220:CDS 65.0%
!! GGTGGTGATGGTGGAGGAGG+AGG - chr8_2:2313295-2313314 Msa1227220:CDS 65.0%
!! GGTGATGGTGGAGGAGGAGG+TGG - chr8_2:2313298-2313317 Msa1227220:CDS 65.0%
!! GATGGTGGAGGAGGAGGTGG+TGG - chr8_2:2313301-2313320 Msa1227220:CDS 65.0%
!! GTGGTGGTGGTGGTGGTGGA+TGG - chr8_2:2313326-2313345 Msa1227220:CDS 65.0%
!! GGTGGTGGTGGTGGTGGATG+GGG - chr8_2:2313328-2313347 Msa1227220:CDS 65.0%
!! GTGGTGGTGGTGGATGGGGA+TGG - chr8_2:2313332-2313351 Msa1227220:CDS 65.0%
!! GGTGGTGGTGGATGGGGATG+GGG - chr8_2:2313334-2313353 Msa1227220:CDS 65.0%
GATGGGGAGGAGGAGGTGGA+GGG - chr8_2:2313350-2313369 Msa1227220:CDS 65.0%
ATGGGGAGGAGGAGGTGGAG+GGG - chr8_2:2313351-2313370 Msa1227220:CDS 65.0%
TGGGGATGGGGTGGAGGAGG+TGG - chr8_2:2313271-2313290 Msa1227220:CDS 70.0%
GGATGGGGTGGAGGAGGTGG+AGG - chr8_2:2313274-2313293 Msa1227220:CDS 70.0%
TGGGGTGGAGGAGGTGGAGG+TGG - chr8_2:2313277-2313296 Msa1227220:CDS 70.0%
! GGTGGAGGAGGAGGTGGTGG+TGG - chr8_2:2313304-2313323 Msa1227220:CDS 70.0%
!! GGAGGAGGAGGTGGTGGTGG+TGG - chr8_2:2313307-2313326 Msa1227220:CDS 70.0%
!! GGAGGAGGTGGTGGTGGTGG+TGG - chr8_2:2313310-2313329 Msa1227220:CDS 70.0%
!! GGAGGTGGTGGTGGTGGTGG+TGG - chr8_2:2313313-2313332 Msa1227220:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr8_2:2313316-2313335 Msa1227220:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr8_2:2313316-2313335 Msa1227220:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr8_2:2313316-2313335 Msa1227220:CDS 70.0%
!! GGTGGTGGATGGGGATGGGG+AGG - chr8_2:2313337-2313356 Msa1227220:CDS 70.0%
GGTGGATGGGGATGGGGAGG+AGG - chr8_2:2313340-2313359 Msa1227220:CDS 70.0%
GGATGGGGATGGGGAGGAGG+AGG - chr8_2:2313343-2313362 Msa1227220:CDS 70.0%
TGGGGATGGGGAGGAGGAGG+TGG - chr8_2:2313346-2313365 Msa1227220:CDS 70.0%
GGATGGGGAGGAGGAGGTGG+AGG - chr8_2:2313349-2313368 Msa1227220:CDS 70.0%
TGGGGAGGAGGAGGTGGAGG+GGG - chr8_2:2313352-2313371 Msa1227220:CDS 70.0%
GAGGAGGTGGAGGGGGTGGT+TGG - chr8_2:2313359-2313378 Msa1227220:CDS 70.0%
GAGGTGGAGGGGGTGGTTGG+TGG - chr8_2:2313362-2313381 Msa1227220:CDS 70.0%
GGAGGAGGAGGTGGAGGGGG+TGG - chr8_2:2313355-2313374 Msa1227220:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 2313031 2313630 2313031 ID=Msa1227220;Name=Msa1227220
chr8_2 mRNA 2313031 2313630 2313031 ID=Msa1227220-mRNA-1;Parent=Msa1227220;Name=Msa1227220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|199
chr8_2 exon 2313031 2313630 2313031 ID=Msa1227220-mRNA-1:exon:607;Parent=Msa1227220-mRNA-1
chr8_2 CDS 2313031 2313630 2313031 ID=Msa1227220-mRNA-1:cds;Parent=Msa1227220-mRNA-1
Gene Sequence

>Msa1227220

ATGGAGAACTTGAGATTTTGTACTGTTCTCTGCTTGATAATTCTGACAGTGGAAATTGTTGGTCTTAAAAGTGACGAGCTGAAAAATGACACTGCTCAAAGTCAAGGCAATGTCACTTTAGCAACCCTCTCTAATCATGAGGCTAGTAAGGAACATGTAAGAGGAAAGCACAACAATGAAGTTGTGTTCAACACAAGCAAAGGTAGCTGGTATAAGAGAGGAGGAAGTGGAGGATTTAAATGGGGATGGGGTGGAGGAGGTGGAGGTGGTGATGGTGGAGGAGGAGGTGGTGGTGGTGGTGGTGGTGGTGGTGGATGGGGATGGGGAGGAGGAGGTGGAGGGGGTGGTTGGTGGAAATGGGGGTGTAGAGGTGAACCAAGATCACATGTTAAAGGGAAGAGAGGAACTATAAAAGGCATGAAGCATCATCATCAACAACATCAACATGCATCCAAGGAAGAATATAGGTTAGGGGAGTTTGCACAATGCATGACAAGAACAAGGTGCAAAGGTATGAGGTTGGATTGCCCTCTTCACTGTGGTGGACCTTGTTTCTATGACTGCTACCATATGTGCAAAGCTCATTGTCGACGACCCTAA

Protein sequence

>Msa1227220

MENLRFCTVLCLIILTVEIVGLKSDELKNDTAQSQGNVTLATLSNHEASKEHVRGKHNNEVVFNTSKGSWYKRGGSGGFKWGWGGGGGGGDGGGGGGGGGGGGGGWGWGGGGGGGGWWKWGCRGEPRSHVKGKRGTIKGMKHHHQQHQHASKEEYRLGEFAQCMTRTRCKGMRLDCPLHCGGPCFYDCYHMCKAHCRRP*