AlfalfaGEDB | Msa1227220

M. sativa cultivar zhongmu-4 / Msa1227220

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227220	XP_003589353.1	90.000	200	14	3	1	199	1	195	6.35e-89	269

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227220	G7I5W6	90.000	200	14	3	1	199	1	195	3.03e-89	269

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0043210	Msa1227220	0.820839	5.548175e-53	-8.615850e-47
Msa0073670	Msa1227220	0.806383	8.448443e-50	-8.615850e-47
Msa0083920	Msa1227220	0.819410	1.178411e-52	-8.615850e-47
Msa0137150	Msa1227220	0.810358	1.199140e-50	-8.615850e-47
Msa0197420	Msa1227220	0.803880	2.822421e-49	-8.615850e-47
Msa0197930	Msa1227220	0.811601	6.451162e-51	-8.615850e-47
Msa0317280	Msa1227220	0.807580	4.716401e-50	-8.615850e-47
Msa0344570	Msa1227220	0.807134	5.863825e-50	-8.615850e-47
Msa0392490	Msa1227220	0.816155	6.399107e-52	-8.615850e-47
Msa0453520	Msa1227220	0.806297	8.808316e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227220	MtrunA17_Chr1g0155831	90.000	200	14	3	1	199	1	195	5.84e-93	269
Msa1227220	MtrunA17_Chr3g0142231	54.930	213	75	8	1	198	1	207	9.76e-32	114

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 69 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTAGCTGGTATAAGAG+AGG	0.636029	8_2:-2313410	Msa1227220:CDS
AAATGGGGATGGGGTGGAGG+AGG	0.395075	8_2:-2313371	Msa1227220:CDS
AACCAAGATCACATGTTAAA+GGG	0.461053	8_2:-2313235	Msa1227220:CDS
AAGGTCCACCACAGTGAAGA+GGG	0.578681	8_2:+2313081	None:intergenic
AAGGTGCAAAGGTATGAGGT+TGG	0.596084	8_2:-2313108	Msa1227220:CDS
AATGACACTGCTCAAAGTCA+AGG	0.622542	8_2:-2313524	Msa1227220:CDS
ACAACATCAACATGCATCCA+AGG	0.561027	8_2:-2313174	Msa1227220:CDS
ACTAGCCTCATGATTAGAGA+GGG	0.605706	8_2:+2313484	None:intergenic
AGCAACCCTCTCTAATCATG+AGG	0.635131	8_2:-2313489	Msa1227220:CDS
AGGAAGTGGAGGATTTAAAT+GGG	0.430452	8_2:-2313387	Msa1227220:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCAAGATCACATGTTAAA+GGG	-	chr8_2:2313404-2313423	Msa1227220:CDS	30.0%
ATTCTGACAGTGGAAATTGT+TGG	-	chr8_2:2313070-2313089	Msa1227220:CDS	35.0%
AGGAAGTGGAGGATTTAAAT+GGG	-	chr8_2:2313252-2313271	Msa1227220:CDS	35.0%
GAACCAAGATCACATGTTAA+AGG	-	chr8_2:2313403-2313422	Msa1227220:CDS	35.0%
TTCCCTTTAACATGTGATCT+TGG	+	chr8_2:2313409-2313428	None:intergenic	35.0%
ATGCATCCAAGGAAGAATAT+AGG	-	chr8_2:2313476-2313495	Msa1227220:CDS	35.0%
TCCAAGGAAGAATATAGGTT+AGG	-	chr8_2:2313481-2313500	Msa1227220:CDS	35.0%
CCAAGGAAGAATATAGGTTA+GGG	-	chr8_2:2313482-2313501	Msa1227220:CDS	35.0%
CAAGGAAGAATATAGGTTAG+GGG	-	chr8_2:2313483-2313502	Msa1227220:CDS	35.0%
AATGACACTGCTCAAAGTCA+AGG	-	chr8_2:2313115-2313134	Msa1227220:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	2313031	2313630	2313031	ID=Msa1227220;Name=Msa1227220
chr8_2	mRNA	2313031	2313630	2313031	ID=Msa1227220-mRNA-1;Parent=Msa1227220;Name=Msa1227220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|199
chr8_2	exon	2313031	2313630	2313031	ID=Msa1227220-mRNA-1:exon:607;Parent=Msa1227220-mRNA-1
chr8_2	CDS	2313031	2313630	2313031	ID=Msa1227220-mRNA-1:cds;Parent=Msa1227220-mRNA-1

Gene Sequence

>Msa1227220

ATGGAGAACTTGAGATTTTGTACTGTTCTCTGCTTGATAATTCTGACAGTGGAAATTGTTGGTCTTAAAAGTGACGAGCTGAAAAATGACACTGCTCAAAGTCAAGGCAATGTCACTTTAGCAACCCTCTCTAATCATGAGGCTAGTAAGGAACATGTAAGAGGAAAGCACAACAATGAAGTTGTGTTCAACACAAGCAAAGGTAGCTGGTATAAGAGAGGAGGAAGTGGAGGATTTAAATGGGGATGGGGTGGAGGAGGTGGAGGTGGTGATGGTGGAGGAGGAGGTGGTGGTGGTGGTGGTGGTGGTGGTGGATGGGGATGGGGAGGAGGAGGTGGAGGGGGTGGTTGGTGGAAATGGGGGTGTAGAGGTGAACCAAGATCACATGTTAAAGGGAAGAGAGGAACTATAAAAGGCATGAAGCATCATCATCAACAACATCAACATGCATCCAAGGAAGAATATAGGTTAGGGGAGTTTGCACAATGCATGACAAGAACAAGGTGCAAAGGTATGAGGTTGGATTGCCCTCTTCACTGTGGTGGACCTTGTTTCTATGACTGCTACCATATGTGCAAAGCTCATTGTCGACGACCCTAA

Protein sequence

>Msa1227220

MENLRFCTVLCLIILTVEIVGLKSDELKNDTAQSQGNVTLATLSNHEASKEHVRGKHNNEVVFNTSKGSWYKRGGSGGFKWGWGGGGGGGDGGGGGGGGGGGGGGWGWGGGGGGGGWWKWGCRGEPRSHVKGKRGTIKGMKHHHQQHQHASKEEYRLGEFAQCMTRTRCKGMRLDCPLHCGGPCFYDCYHMCKAHCRRP*