AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0936340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0936340 MtrunA17_Chr1g0155831 84.577 201 24 4 1 200 1 195 4.19e-90 262
Msa0936340 MtrunA17_Chr3g0142231 55.189 212 77 8 1 199 1 207 4.62e-33 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 74 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAAATTGTTGGTCTTAA+AGG 0.180821 6_3:-7012739 Msa0936340:CDS
GGTGGTGGTGGTGGAGGAGG+AGG 0.223597 6_3:-7012514 Msa0936340:CDS
GGTGGAGGTGGTGGTGGTGG+TGG 0.225391 6_3:-7012523 Msa0936340:CDS
TGGGGATGGGGAGGAGGTGG+AGG 0.225555 6_3:-7012466 Msa0936340:CDS
GGTGGTGGTGGAGGAGGAGG+AGG 0.229961 6_3:-7012511 Msa0936340:CDS
GGTGGTGGTGGATGGGGATG+GGG 0.237687 6_3:-7012478 Msa0936340:CDS
GAGGAAGTGGAGGATTTAAA+TGG 0.248104 6_3:-7012567 Msa0936340:CDS
GGAGGAGGTGGAGGTGGAGG+TGG 0.248281 6_3:-7012535 Msa0936340:CDS
GGATGGGGATGGGGAGGAGG+TGG 0.250421 6_3:-7012469 Msa0936340:CDS
TGGGGTGGAGGAGGTGGAGG+TGG 0.253492 6_3:-7012541 Msa0936340:CDS
GGAGGAGGAGGTGGAGGTGG+TGG 0.253619 6_3:-7012493 Msa0936340:CDS
GGTGGTGGAGGAGGAGGAGG+AGG 0.256079 6_3:-7012508 Msa0936340:CDS
GGTGGAGGTGGAGGTGGTGG+TGG 0.264446 6_3:-7012529 Msa0936340:CDS
GGAGGAGGAGGAGGTGGAGG+TGG 0.265820 6_3:-7012496 Msa0936340:CDS
GGAGGTGGAGGTGGTGGTGG+TGG 0.265833 6_3:-7012526 Msa0936340:CDS
GGTGGATGGGGATGGGGAGG+AGG 0.267297 6_3:-7012472 Msa0936340:CDS
GGAGGTGGAGGTGGAGGTGG+TGG 0.267483 6_3:-7012532 Msa0936340:CDS
GAGGTGGAGGTGGTGGTGGA+TGG 0.268411 6_3:-7012486 Msa0936340:CDS
GGTGGAGGTGGTGGTGGATG+GGG 0.268933 6_3:-7012484 Msa0936340:CDS
GGAGGAGGTGGAGGTGGTGG+TGG 0.280221 6_3:-7012490 Msa0936340:CDS
GGTTGGGGTGGAGGAGGTGG+AGG 0.298059 6_3:-7012544 Msa0936340:CDS
GGTGGAGGAGGTGGAGGTGG+AGG 0.299482 6_3:-7012538 Msa0936340:CDS
AGGTGGTGGTGGATGGGGAT+GGG 0.302309 6_3:-7012479 Msa0936340:CDS
TGGGGTTGGGGTGGAGGAGG+TGG 0.303215 6_3:-7012547 Msa0936340:CDS
TTTAAATGGGGTTGGGGTGG+AGG 0.303957 6_3:-7012553 Msa0936340:CDS
GGATGGGGAGGAGGTGGAGG+GGG 0.307987 6_3:-7012463 Msa0936340:CDS
CAACCAAGATCACATGTTAA+AGG 0.321091 6_3:-7012412 Msa0936340:CDS
AGGTGGAGGTGGTGGTGGAT+GGG 0.323646 6_3:-7012485 Msa0936340:CDS
TTCCCTTTAACATGTGATCT+TGG 0.328344 6_3:+7012409 None:intergenic
GGGGTGGTTGGTGGAAATGG+GGG 0.329480 6_3:-7012444 Msa0936340:CDS
GAGGTGGTGGTGGATGGGGA+TGG 0.333518 6_3:-7012480 Msa0936340:CDS
GGTGGTGGATGGGGATGGGG+AGG 0.340139 6_3:-7012475 Msa0936340:CDS
GGATTTAAATGGGGTTGGGG+TGG 0.350065 6_3:-7012556 Msa0936340:CDS
GGGATGGGGAGGAGGTGGAG+GGG 0.362069 6_3:-7012464 Msa0936340:CDS
AAATGGGGTTGGGGTGGAGG+AGG 0.372826 6_3:-7012550 Msa0936340:CDS
TGGAGGATTTAAATGGGGTT+GGG 0.377765 6_3:-7012560 Msa0936340:CDS
GACAGTGAGCTTTGCACATA+TGG 0.410730 6_3:+7012218 None:intergenic
TGGTATAAGAGAGGAGGAAG+TGG 0.415386 6_3:-7012580 Msa0936340:CDS
GGGGATGGGGAGGAGGTGGA+GGG 0.422054 6_3:-7012465 Msa0936340:CDS
TCCAAGGAAGAGTATAGGTT+GGG 0.425749 6_3:-7012334 Msa0936340:CDS
GGAGGATTTAAATGGGGTTG+GGG 0.427078 6_3:-7012559 Msa0936340:CDS
AACCAAGATCACATGTTAAA+GGG 0.448682 6_3:-7012411 Msa0936340:CDS
AGGAAGTGGAGGATTTAAAT+GGG 0.457174 6_3:-7012566 Msa0936340:CDS
GTGGAGGATTTAAATGGGGT+TGG 0.480254 6_3:-7012561 Msa0936340:CDS
CCCCAACCTATACTCTTCCT+TGG 0.496096 6_3:+7012333 None:intergenic
GCTAGTGAGGAGCATGTAAG+AGG 0.499009 6_3:-7012646 Msa0936340:CDS
TATAAGAGAGGAGGAAGTGG+AGG 0.499057 6_3:-7012577 Msa0936340:CDS
TTAACACAAGCAAAGGTAGT+TGG 0.520660 6_3:-7012600 Msa0936340:CDS
CTCTAATCATGAGGCTAGTG+AGG 0.540660 6_3:-7012659 Msa0936340:CDS
ATGCATCCAAGGAAGAGTAT+AGG 0.541399 6_3:-7012339 Msa0936340:CDS
ATTCTGACAGTGGAAATTGT+TGG 0.544581 6_3:-7012748 Msa0936340:CDS
AAAGGTAGTTGGTATAAGAG+AGG 0.548731 6_3:-7012589 Msa0936340:CDS
AAAAGGAACTATAAGGGCCA+TGG 0.552884 6_3:-7012386 Msa0936340:CDS
ACAACATCAACATGCATCCA+AGG 0.569790 6_3:-7012350 Msa0936340:CDS
CCAAGGAAGAGTATAGGTTG+GGG 0.576804 6_3:-7012333 Msa0936340:CDS
GTTGTGTTTAACACAAGCAA+AGG 0.578822 6_3:-7012607 Msa0936340:CDS
GGTAGTTGGTATAAGAGAGG+AGG 0.581653 6_3:-7012586 Msa0936340:CDS
AAGGTGCAAAGGTCTGAGGT+TGG 0.591346 6_3:-7012284 Msa0936340:CDS
ATCCAAGGAAGAGTATAGGT+TGG 0.594810 6_3:-7012335 Msa0936340:CDS
GATTGTCCTCTTCACTGTGG+TGG 0.599999 6_3:-7012262 Msa0936340:CDS
CACTAGCCTCATGATTAGAG+AGG 0.605140 6_3:+7012662 None:intergenic
CACAATGCATGACAAGAACA+AGG 0.612043 6_3:-7012303 Msa0936340:CDS
CTGCTTGATAATTCTGACAG+TGG 0.615962 6_3:-7012758 Msa0936340:CDS
GAACAAGGTGCAAAGGTCTG+AGG 0.618760 6_3:-7012288 Msa0936340:CDS
CAAGGTCCACCACAGTGAAG+AGG 0.621208 6_3:+7012256 None:intergenic
AATGACACTGCTCAAAGTCA+AGG 0.622542 6_3:-7012703 Msa0936340:CDS
TTGTTGACGATGATGCTCCA+TGG 0.628464 6_3:+7012369 None:intergenic
ATGACAAGAACAAGGTGCAA+AGG 0.629057 6_3:-7012295 Msa0936340:CDS
ACTAGCCTCATGATTAGAGA+GGG 0.636269 6_3:+7012663 None:intergenic
AGTAACCCTCTCTAATCATG+AGG 0.642546 6_3:-7012668 Msa0936340:CDS
GGAAGTGGAGGATTTAAATG+GGG 0.653528 6_3:-7012565 Msa0936340:CDS
TGGTAGCAGTCATAGAAACA+AGG 0.674985 6_3:+7012238 None:intergenic
TTGGATTGTCCTCTTCACTG+TGG 0.685033 6_3:-7012265 Msa0936340:CDS
CAAGGAAGAGTATAGGTTGG+GGG 0.693571 6_3:-7012332 Msa0936340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACCAAGATCACATGTTAAA+GGG - chr6_3:7012583-7012602 Msa0936340:CDS 30.0%
TCACATGTTAAAGGGAAAAA+AGG - chr6_3:7012591-7012610 Msa0936340:CDS 30.0%
AAGGGAAAAAAGGAACTATA+AGG - chr6_3:7012601-7012620 Msa0936340:CDS 30.0%
AGGGAAAAAAGGAACTATAA+GGG - chr6_3:7012602-7012621 Msa0936340:CDS 30.0%
ATTCTGACAGTGGAAATTGT+TGG - chr6_3:7012246-7012265 Msa0936340:CDS 35.0%
!! GTGGAAATTGTTGGTCTTAA+AGG - chr6_3:7012255-7012274 Msa0936340:CDS 35.0%
GTTGTGTTTAACACAAGCAA+AGG - chr6_3:7012387-7012406 Msa0936340:CDS 35.0%
TTAACACAAGCAAAGGTAGT+TGG - chr6_3:7012394-7012413 Msa0936340:CDS 35.0%
! AAAGGTAGTTGGTATAAGAG+AGG - chr6_3:7012405-7012424 Msa0936340:CDS 35.0%
AGGAAGTGGAGGATTTAAAT+GGG - chr6_3:7012428-7012447 Msa0936340:CDS 35.0%
CAACCAAGATCACATGTTAA+AGG - chr6_3:7012582-7012601 Msa0936340:CDS 35.0%
TTCCCTTTAACATGTGATCT+TGG + chr6_3:7012588-7012607 None:intergenic 35.0%
! CTGCTTGATAATTCTGACAG+TGG - chr6_3:7012236-7012255 Msa0936340:CDS 40.0%
AATGACACTGCTCAAAGTCA+AGG - chr6_3:7012291-7012310 Msa0936340:CDS 40.0%
AGTAACCCTCTCTAATCATG+AGG - chr6_3:7012326-7012345 Msa0936340:CDS 40.0%
! ACTAGCCTCATGATTAGAGA+GGG + chr6_3:7012334-7012353 None:intergenic 40.0%
GAGGAAGTGGAGGATTTAAA+TGG - chr6_3:7012427-7012446 Msa0936340:CDS 40.0%
GGAAGTGGAGGATTTAAATG+GGG - chr6_3:7012429-7012448 Msa0936340:CDS 40.0%
TGGAGGATTTAAATGGGGTT+GGG - chr6_3:7012434-7012453 Msa0936340:CDS 40.0%
AAAAGGAACTATAAGGGCCA+TGG - chr6_3:7012608-7012627 Msa0936340:CDS 40.0%
ACAACATCAACATGCATCCA+AGG - chr6_3:7012644-7012663 Msa0936340:CDS 40.0%
! ATGCATCCAAGGAAGAGTAT+AGG - chr6_3:7012655-7012674 Msa0936340:CDS 40.0%
! ATCCAAGGAAGAGTATAGGT+TGG - chr6_3:7012659-7012678 Msa0936340:CDS 40.0%
! TCCAAGGAAGAGTATAGGTT+GGG - chr6_3:7012660-7012679 Msa0936340:CDS 40.0%
CACAATGCATGACAAGAACA+AGG - chr6_3:7012691-7012710 Msa0936340:CDS 40.0%
ATGACAAGAACAAGGTGCAA+AGG - chr6_3:7012699-7012718 Msa0936340:CDS 40.0%
TGGTAGCAGTCATAGAAACA+AGG + chr6_3:7012759-7012778 None:intergenic 40.0%
! CACTAGCCTCATGATTAGAG+AGG + chr6_3:7012335-7012354 None:intergenic 45.0%
CTCTAATCATGAGGCTAGTG+AGG - chr6_3:7012335-7012354 Msa0936340:CDS 45.0%
! GGTAGTTGGTATAAGAGAGG+AGG - chr6_3:7012408-7012427 Msa0936340:CDS 45.0%
TGGTATAAGAGAGGAGGAAG+TGG - chr6_3:7012414-7012433 Msa0936340:CDS 45.0%
TATAAGAGAGGAGGAAGTGG+AGG - chr6_3:7012417-7012436 Msa0936340:CDS 45.0%
GTGGAGGATTTAAATGGGGT+TGG - chr6_3:7012433-7012452 Msa0936340:CDS 45.0%
GGAGGATTTAAATGGGGTTG+GGG - chr6_3:7012435-7012454 Msa0936340:CDS 45.0%
TTGTTGACGATGATGCTCCA+TGG + chr6_3:7012628-7012647 None:intergenic 45.0%
! CCAAGGAAGAGTATAGGTTG+GGG - chr6_3:7012661-7012680 Msa0936340:CDS 45.0%
! CAAGGAAGAGTATAGGTTGG+GGG - chr6_3:7012662-7012681 Msa0936340:CDS 45.0%
TTGGATTGTCCTCTTCACTG+TGG - chr6_3:7012729-7012748 Msa0936340:CDS 45.0%
GACAGTGAGCTTTGCACATA+TGG + chr6_3:7012779-7012798 None:intergenic 45.0%
! GCTAGTGAGGAGCATGTAAG+AGG - chr6_3:7012348-7012367 Msa0936340:CDS 50.0%
GGATTTAAATGGGGTTGGGG+TGG - chr6_3:7012438-7012457 Msa0936340:CDS 50.0%
TTTAAATGGGGTTGGGGTGG+AGG - chr6_3:7012441-7012460 Msa0936340:CDS 50.0%
CCCCAACCTATACTCTTCCT+TGG + chr6_3:7012664-7012683 None:intergenic 50.0%
GAACAAGGTGCAAAGGTCTG+AGG - chr6_3:7012706-7012725 Msa0936340:CDS 50.0%
! AAGGTGCAAAGGTCTGAGGT+TGG - chr6_3:7012710-7012729 Msa0936340:CDS 50.0%
GATTGTCCTCTTCACTGTGG+TGG - chr6_3:7012732-7012751 Msa0936340:CDS 50.0%
!! AGGGGGTGGTTGGTGGAAAT+GGG - chr6_3:7012548-7012567 Msa0936340:CDS 55.0%
! TGGTGGAAATGGGGGTGTAG+AGG - chr6_3:7012558-7012577 Msa0936340:CDS 55.0%
CAAGGTCCACCACAGTGAAG+AGG + chr6_3:7012741-7012760 None:intergenic 55.0%
AAATGGGGTTGGGGTGGAGG+AGG - chr6_3:7012444-7012463 Msa0936340:CDS 60.0%
!! AGGTGGAGGTGGTGGTGGAT+GGG - chr6_3:7012509-7012528 Msa0936340:CDS 60.0%
!! AGGTGGTGGTGGATGGGGAT+GGG - chr6_3:7012515-7012534 Msa0936340:CDS 60.0%
!! GAGGGGGTGGTTGGTGGAAA+TGG - chr6_3:7012547-7012566 Msa0936340:CDS 60.0%
!! GGGGGTGGTTGGTGGAAATG+GGG - chr6_3:7012549-7012568 Msa0936340:CDS 60.0%
!! GGGGTGGTTGGTGGAAATGG+GGG - chr6_3:7012550-7012569 Msa0936340:CDS 60.0%
!! GAGGTGGAGGTGGTGGTGGA+TGG - chr6_3:7012508-7012527 Msa0936340:CDS 65.0%
!! GGTGGAGGTGGTGGTGGATG+GGG - chr6_3:7012510-7012529 Msa0936340:CDS 65.0%
!! GAGGTGGTGGTGGATGGGGA+TGG - chr6_3:7012514-7012533 Msa0936340:CDS 65.0%
!! GGTGGTGGTGGATGGGGATG+GGG - chr6_3:7012516-7012535 Msa0936340:CDS 65.0%
TGGGGTTGGGGTGGAGGAGG+TGG - chr6_3:7012447-7012466 Msa0936340:CDS 70.0%
GGTTGGGGTGGAGGAGGTGG+AGG - chr6_3:7012450-7012469 Msa0936340:CDS 70.0%
TGGGGTGGAGGAGGTGGAGG+TGG - chr6_3:7012453-7012472 Msa0936340:CDS 70.0%
GGTGGAGGAGGTGGAGGTGG+AGG - chr6_3:7012456-7012475 Msa0936340:CDS 70.0%
GGAGGAGGTGGAGGTGGAGG+TGG - chr6_3:7012459-7012478 Msa0936340:CDS 70.0%
GGAGGTGGAGGTGGAGGTGG+TGG - chr6_3:7012462-7012481 Msa0936340:CDS 70.0%
! GGTGGAGGTGGAGGTGGTGG+TGG - chr6_3:7012465-7012484 Msa0936340:CDS 70.0%
!! GGAGGTGGAGGTGGTGGTGG+TGG - chr6_3:7012468-7012487 Msa0936340:CDS 70.0%
!! GGTGGAGGTGGTGGTGGTGG+TGG - chr6_3:7012471-7012490 Msa0936340:CDS 70.0%
!! GGAGGTGGTGGTGGTGGTGG+AGG - chr6_3:7012474-7012493 Msa0936340:CDS 70.0%
!! GGTGGTGGTGGTGGTGGAGG+AGG - chr6_3:7012477-7012496 Msa0936340:CDS 70.0%
!! GGTGGTGGTGGTGGAGGAGG+AGG - chr6_3:7012480-7012499 Msa0936340:CDS 70.0%
!! GGTGGTGGTGGAGGAGGAGG+AGG - chr6_3:7012483-7012502 Msa0936340:CDS 70.0%
!! GGTGGTGGAGGAGGAGGAGG+AGG - chr6_3:7012486-7012505 Msa0936340:CDS 70.0%
GGTGGAGGAGGAGGAGGAGG+AGG - chr6_3:7012489-7012508 Msa0936340:CDS 70.0%
GGAGGAGGAGGAGGAGGAGG+TGG - chr6_3:7012492-7012511 Msa0936340:CDS 70.0%
GGAGGAGGAGGAGGAGGTGG+AGG - chr6_3:7012495-7012514 Msa0936340:CDS 70.0%
GGAGGAGGAGGAGGTGGAGG+TGG - chr6_3:7012498-7012517 Msa0936340:CDS 70.0%
GGAGGAGGAGGTGGAGGTGG+TGG - chr6_3:7012501-7012520 Msa0936340:CDS 70.0%
! GGAGGAGGTGGAGGTGGTGG+TGG - chr6_3:7012504-7012523 Msa0936340:CDS 70.0%
!! GGTGGTGGATGGGGATGGGG+AGG - chr6_3:7012519-7012538 Msa0936340:CDS 70.0%
GGTGGATGGGGATGGGGAGG+AGG - chr6_3:7012522-7012541 Msa0936340:CDS 70.0%
GGATGGGGATGGGGAGGAGG+TGG - chr6_3:7012525-7012544 Msa0936340:CDS 70.0%
TGGGGATGGGGAGGAGGTGG+AGG - chr6_3:7012528-7012547 Msa0936340:CDS 70.0%
GGGGATGGGGAGGAGGTGGA+GGG - chr6_3:7012529-7012548 Msa0936340:CDS 70.0%
GGGATGGGGAGGAGGTGGAG+GGG - chr6_3:7012530-7012549 Msa0936340:CDS 70.0%
GGATGGGGAGGAGGTGGAGG+GGG - chr6_3:7012531-7012550 Msa0936340:CDS 70.0%
GAGGAGGTGGAGGGGGTGGT+TGG - chr6_3:7012538-7012557 Msa0936340:CDS 70.0%
GAGGTGGAGGGGGTGGTTGG+TGG - chr6_3:7012541-7012560 Msa0936340:CDS 70.0%
TGGGGAGGAGGTGGAGGGGG+TGG - chr6_3:7012534-7012553 Msa0936340:CDS 75.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 7012207 7012809 7012207 ID=Msa0936340;Name=Msa0936340
chr6_3 mRNA 7012207 7012809 7012207 ID=Msa0936340-mRNA-1;Parent=Msa0936340;Name=Msa0936340-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|200
chr6_3 exon 7012207 7012809 7012207 ID=Msa0936340-mRNA-1:exon:2297;Parent=Msa0936340-mRNA-1
chr6_3 CDS 7012207 7012809 7012207 ID=Msa0936340-mRNA-1:cds;Parent=Msa0936340-mRNA-1
Gene Sequence

>Msa0936340

ATGGAGAACTTGAGATTTTGTACTGTTCTCTGCTTGATAATTCTGACAGTGGAAATTGTTGGTCTTAAAGGTGACGAGCTGAAAAATGACACTGCTCAAAGTCAAGGCAATGTCACTTTAGTAACCCTCTCTAATCATGAGGCTAGTGAGGAGCATGTAAGAGGAAAACACAACAATGAAGTTGTGTTTAACACAAGCAAAGGTAGTTGGTATAAGAGAGGAGGAAGTGGAGGATTTAAATGGGGTTGGGGTGGAGGAGGTGGAGGTGGAGGTGGTGGTGGTGGTGGAGGAGGAGGAGGAGGAGGTGGAGGTGGTGGTGGATGGGGATGGGGAGGAGGTGGAGGGGGTGGTTGGTGGAAATGGGGGTGTAGAGGTCAACCAAGATCACATGTTAAAGGGAAAAAAGGAACTATAAGGGCCATGGAGCATCATCGTCAACAACATCAACATGCATCCAAGGAAGAGTATAGGTTGGGGGAGTTTGCACAATGCATGACAAGAACAAGGTGCAAAGGTCTGAGGTTGGATTGTCCTCTTCACTGTGGTGGACCTTGTTTCTATGACTGCTACCATATGTGCAAAGCTCACTGTCGACGACCCTAA

Protein sequence

>Msa0936340

MENLRFCTVLCLIILTVEIVGLKGDELKNDTAQSQGNVTLVTLSNHEASEEHVRGKHNNEVVFNTSKGSWYKRGGSGGFKWGWGGGGGGGGGGGGGGGGGGGGGGGGWGWGGGGGGGWWKWGCRGQPRSHVKGKKGTIRAMEHHRQQHQHASKEEYRLGEFAQCMTRTRCKGLRLDCPLHCGGPCFYDCYHMCKAHCRRP*