AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1370870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370870 MtrunA17_Chr1g0195561 93.401 197 13 0 1 197 1 197 1.42e-135 377
Msa1370870 MtrunA17_Chr7g0269361 68.927 177 53 1 22 196 21 197 4.93e-88 257
Msa1370870 MtrunA17_Chr4g0067891 35.437 206 118 4 1 191 1 206 7.01e-36 124
Msa1370870 MtrunA17_Chr4g0018251 35.789 190 105 3 11 189 12 195 4.04e-34 120
Msa1370870 MtrunA17_Chr8g0391341 34.419 215 117 5 1 191 1 215 9.17e-34 119
Msa1370870 MtrunA17_Chr4g0018281 34.715 193 109 3 8 189 9 195 1.02e-33 119
Msa1370870 MtrunA17_Chr4g0018241 34.715 193 109 3 8 189 9 195 1.03e-32 116
Msa1370870 MtrunA17_Chr3g0079401 32.500 160 104 2 33 188 26 185 2.51e-29 107
Msa1370870 MtrunA17_Chr2g0301381 39.181 171 92 4 27 192 29 192 9.57e-29 106
Msa1370870 MtrunA17_Chr5g0398871 31.351 185 119 3 10 189 29 210 7.14e-28 104
Msa1370870 MtrunA17_Chr3g0079381 30.625 160 107 2 33 188 25 184 1.63e-27 102
Msa1370870 MtrunA17_Chr4g0070161 30.159 189 120 5 7 186 11 196 3.60e-22 89.4
Msa1370870 MtrunA17_Chr4g0056601 26.000 200 136 4 1 188 1 200 8.61e-21 85.5
Msa1370870 MtrunA17_Chr4g0011711 32.558 172 95 5 33 188 33 199 6.23e-20 83.6
Msa1370870 MtrunA17_Chr4g0011701 32.558 172 95 5 33 188 33 199 9.89e-20 83.2
Msa1370870 MtrunA17_Chr4g0011681 31.156 199 113 7 8 188 7 199 1.93e-19 82.4
Msa1370870 MtrunA17_Chr4g0011721 29.000 200 119 6 5 188 7 199 6.46e-18 78.2
Msa1370870 MtrunA17_Chr3g0079371 26.415 159 110 4 33 185 67 224 1.26e-16 75.1
Msa1370870 MtrunA17_Chr7g0230391 28.736 174 104 5 28 184 39 209 5.02e-16 73.6
Msa1370870 MtrunA17_Chr3g0092681 28.652 178 115 5 8 183 54 221 1.11e-14 69.7
Msa1370870 MtrunA17_Chr4g0018231 28.750 160 108 3 33 187 44 202 3.19e-14 68.2
Msa1370870 MtrunA17_Chr8g0345211 28.571 161 104 3 32 186 76 231 8.41e-14 68.9
Msa1370870 MtrunA17_Chr7g0272141 26.943 193 124 5 8 186 5 194 1.56e-13 68.2
Msa1370870 MtrunA17_Chr4g0067611 26.875 160 113 2 28 187 54 209 1.89e-13 68.2
Msa1370870 MtrunA17_Chr8g0345281 30.818 159 105 3 33 186 68 226 2.12e-13 67.8
Msa1370870 MtrunA17_Chr1g0147051 27.746 173 112 3 22 182 71 242 1.15e-12 65.9
Msa1370870 MtrunA17_Chr1g0192311 27.950 161 108 3 33 189 58 214 1.17e-12 65.9
Msa1370870 MtrunA17_Chr1g0192281 27.950 161 108 3 33 189 58 214 1.17e-12 65.9
Msa1370870 MtrunA17_Chr4g0069891 36.170 94 58 1 95 188 142 233 7.96e-12 63.5
Msa1370870 MtrunA17_Chr7g0272151 24.204 157 117 2 33 189 59 213 1.46e-11 62.8
Msa1370870 MtrunA17_Chr4g0067601 26.875 160 113 1 28 187 54 209 2.06e-11 62.0
Msa1370870 MtrunA17_Chr8g0354901 26.667 165 110 4 33 189 76 237 2.38e-11 62.0
Msa1370870 MtrunA17_Chr2g0295531 25.301 166 108 4 28 186 38 194 5.00e-11 61.2
Msa1370870 MtrunA17_Chr4g0000121 26.543 162 109 4 33 189 45 201 6.04e-11 60.8
Msa1370870 MtrunA17_Chr1g0192301 26.543 162 109 4 33 189 63 219 8.56e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370870 AT3G62820.1 47.619 189 95 2 6 191 7 194 2.18e-56 177
Msa1370870 AT2G47670.1 47.826 161 81 2 33 191 49 208 6.32e-38 130
Msa1370870 AT5G20740.1 35.979 189 112 3 10 189 8 196 7.04e-31 112
Msa1370870 AT4G12390.1 38.365 159 91 2 36 187 43 201 1.33e-30 111
Msa1370870 AT5G20740.2 35.979 189 112 3 10 189 42 230 2.10e-30 112
Msa1370870 AT5G62350.1 34.969 163 100 2 33 189 39 201 2.66e-30 110
Msa1370870 AT5G62360.1 36.076 158 98 2 33 188 44 200 3.31e-30 110
Msa1370870 AT1G62760.1 33.951 162 104 1 31 189 146 307 4.02e-29 110
Msa1370870 AT1G14890.1 32.990 194 112 4 10 190 30 218 2.56e-28 105
Msa1370870 AT4G25260.1 30.769 195 119 5 10 188 5 199 8.32e-28 104
Msa1370870 AT2G01610.1 35.000 180 95 4 31 190 43 220 1.93e-27 103
Msa1370870 AT3G47380.1 35.404 161 98 2 33 187 39 199 4.61e-27 102
Msa1370870 AT1G70720.1 33.505 194 115 4 8 191 9 198 5.05e-25 97.1
Msa1370870 AT1G70720.2 33.505 194 115 4 8 191 21 210 5.32e-25 97.4
Msa1370870 AT1G23205.1 34.737 190 111 4 8 185 10 198 2.01e-24 95.5
Msa1370870 AT1G62770.1 31.875 160 101 4 36 187 37 196 2.14e-24 95.5
Msa1370870 AT4G25250.1 31.013 158 100 4 33 183 38 193 8.67e-19 80.5
Msa1370870 AT3G14310.1 33.333 168 106 4 33 195 59 225 7.99e-18 81.3
Msa1370870 AT5G51520.1 24.752 202 132 5 5 186 1 202 1.84e-16 74.7
Msa1370870 AT4G00080.1 30.120 166 104 5 30 184 33 197 6.41e-15 70.5
Msa1370870 AT1G53840.1 28.571 175 110 5 32 196 72 241 4.71e-14 70.1
Msa1370870 AT3G05610.1 26.452 155 112 2 33 187 58 210 8.14e-14 69.7
Msa1370870 AT1G53830.1 29.940 167 113 3 33 195 62 228 9.81e-14 69.3
Msa1370870 AT5G04960.1 30.120 166 99 4 32 196 67 216 1.87e-13 68.6
Msa1370870 AT4G15980.1 27.152 151 106 2 37 186 52 199 7.63e-13 66.6
Msa1370870 AT4G15980.2 27.152 151 106 2 37 186 62 209 8.82e-13 66.6
Msa1370870 AT5G27870.1 26.061 165 119 3 33 197 57 218 1.78e-12 65.9
Msa1370870 AT3G49220.2 33.077 130 80 3 28 156 127 250 1.12e-11 63.2
Msa1370870 AT3G14300.1 26.347 167 111 4 32 187 270 435 1.20e-11 63.5
Msa1370870 AT5G49180.1 27.564 156 111 2 33 187 55 209 2.32e-11 62.4
Msa1370870 AT3G49220.3 33.077 130 80 3 28 156 127 250 2.41e-11 62.4
Msa1370870 AT3G49220.1 33.077 130 80 3 28 156 82 205 3.60e-11 62.0
Msa1370870 AT3G05620.1 25.434 173 112 4 30 186 30 201 5.79e-11 61.2

Find 37 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGAGCTAGAGCGTTAC+TGG 0.280918 tig0019143:-166464 None:intergenic
GATCAAGATACTTGCCTTGA+TGG 0.282001 tig0019143:+166373 Msa1370870:CDS
CTGCACTTATCCATGTATTA+AGG 0.338152 tig0019143:-166342 None:intergenic
AGCGTTACTGGTTATATAAT+AGG 0.353010 tig0019143:-166452 None:intergenic
ACTTGCCTTGATGGATTTCA+AGG 0.400968 tig0019143:+166382 Msa1370870:CDS
CAGCCCTTGCCCACTTGCTA+GGG 0.415413 tig0019143:-166117 None:intergenic
AGTCAGTTGGTATTTGCAAA+AGG 0.416317 tig0019143:+166001 Msa1370870:CDS
ACTAGACACGGGCATTTAAG+AGG 0.436602 tig0019143:+166196 Msa1370870:CDS
CCTAGCAAGTGGGCAAGGGC+TGG 0.454457 tig0019143:+166118 Msa1370870:CDS
GTTAATAAACTTGCCACTAC+AGG 0.455754 tig0019143:+166484 Msa1370870:CDS
ACCAACTGACTTGCAAGGTA+TGG 0.470021 tig0019143:-165989 None:intergenic
TTAAGTTTGCGAGATATGCT+TGG 0.470762 tig0019143:-166174 None:intergenic
AGGCTAAGTAGAACGACATT+TGG 0.472627 tig0019143:+166304 Msa1370870:CDS
ACGACATTTGGTACCCAGAT+GGG 0.493858 tig0019143:+166316 Msa1370870:CDS
GAAGCCCTAGCAAGTGGGCA+AGG 0.500888 tig0019143:+166113 Msa1370870:CDS
GTCAGTTGGTATTTGCAAAA+GGG 0.511286 tig0019143:+166002 Msa1370870:CDS
CTCGCAAACTTAACTAGACA+CGG 0.515036 tig0019143:+166184 Msa1370870:CDS
GAGAAGCTTGCAAAGTAACT+AGG 0.515953 tig0019143:+166038 Msa1370870:CDS
TCCATACCTTGCAAGTCAGT+TGG 0.518339 tig0019143:+165988 Msa1370870:CDS
AAACTTGCCACTACAGGTCT+AGG 0.519005 tig0019143:+166490 Msa1370870:CDS
GCACCGTTCTCTAACAGTGC+TGG 0.521547 tig0019143:+166088 Msa1370870:CDS
GTGATCTATGAAGCTCATCA+AGG 0.523992 tig0019143:-166267 None:intergenic
GATCACTGAATGTGCTCAGA+AGG 0.546424 tig0019143:+166284 Msa1370870:CDS
GCTGGAGTGTCAGTGACAAT+CGG 0.557488 tig0019143:+166136 Msa1370870:CDS
CAAATACCAACTGACTTGCA+AGG 0.559475 tig0019143:-165994 None:intergenic
AACGACATTTGGTACCCAGA+TGG 0.584170 tig0019143:+166315 Msa1370870:CDS
CGACATTTGGTACCCAGATG+GGG 0.585386 tig0019143:+166317 Msa1370870:CDS
TGATCTATGAAGCTCATCAA+GGG 0.595202 tig0019143:-166266 None:intergenic
CTGGAAGAAGCCCTAGCAAG+TGG 0.599584 tig0019143:+166107 Msa1370870:CDS
CTTCCAGCACTGTTAGAGAA+CGG 0.607318 tig0019143:-166091 None:intergenic
AAGCCCTAGCAAGTGGGCAA+GGG 0.611205 tig0019143:+166114 Msa1370870:CDS
GATGCTTCCTAGACCTGTAG+TGG 0.614161 tig0019143:-166497 None:intergenic
GTGCTAGCGAGCGCGTACAA+AGG 0.625584 tig0019143:-166069 None:intergenic
TAAATGCAAGCAGAAAGGTA+AGG 0.639842 tig0019143:-165958 None:intergenic
GACATTTGGTACCCAGATGG+GGG 0.662357 tig0019143:+166318 Msa1370870:CDS
TGGAAGAAGCCCTAGCAAGT+GGG 0.667217 tig0019143:+166108 Msa1370870:CDS
TCGCAAACTTAACTAGACAC+GGG 0.670532 tig0019143:+166185 Msa1370870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAATAAATGCAAGCAGAA+AGG - tig0019143:165966-165985 None:intergenic 25.0%
! AGCGTTACTGGTTATATAAT+AGG - tig0019143:166455-166474 None:intergenic 30.0%
TAAATGCAAGCAGAAAGGTA+AGG - tig0019143:165961-165980 None:intergenic 35.0%
! AGTCAGTTGGTATTTGCAAA+AGG + tig0019143:166001-166020 Msa1370870:CDS 35.0%
! GTCAGTTGGTATTTGCAAAA+GGG + tig0019143:166002-166021 Msa1370870:CDS 35.0%
TTAAGTTTGCGAGATATGCT+TGG - tig0019143:166177-166196 None:intergenic 35.0%
TGATCTATGAAGCTCATCAA+GGG - tig0019143:166269-166288 None:intergenic 35.0%
CTGCACTTATCCATGTATTA+AGG - tig0019143:166345-166364 None:intergenic 35.0%
! TTTTGCCTTGAAATCCATCA+AGG - tig0019143:166390-166409 None:intergenic 35.0%
! GATGGATTTCAAGGCAAAAA+CGG + tig0019143:166391-166410 Msa1370870:CDS 35.0%
GTTAATAAACTTGCCACTAC+AGG + tig0019143:166484-166503 Msa1370870:CDS 35.0%
!! CAAATACCAACTGACTTGCA+AGG - tig0019143:165997-166016 None:intergenic 40.0%
GAGAAGCTTGCAAAGTAACT+AGG + tig0019143:166038-166057 Msa1370870:CDS 40.0%
CTCGCAAACTTAACTAGACA+CGG + tig0019143:166184-166203 Msa1370870:CDS 40.0%
TCGCAAACTTAACTAGACAC+GGG + tig0019143:166185-166204 Msa1370870:CDS 40.0%
GTGATCTATGAAGCTCATCA+AGG - tig0019143:166270-166289 None:intergenic 40.0%
AGGCTAAGTAGAACGACATT+TGG + tig0019143:166304-166323 Msa1370870:CDS 40.0%
GATCAAGATACTTGCCTTGA+TGG + tig0019143:166373-166392 Msa1370870:CDS 40.0%
!! ACTTGCCTTGATGGATTTCA+AGG + tig0019143:166382-166401 Msa1370870:CDS 40.0%
GATTTCAAGGCAAAAACGGT+AGG + tig0019143:166395-166414 Msa1370870:CDS 40.0%
TCCATACCTTGCAAGTCAGT+TGG + tig0019143:165988-166007 Msa1370870:CDS 45.0%
!! ACCAACTGACTTGCAAGGTA+TGG - tig0019143:165992-166011 None:intergenic 45.0%
CTTCCAGCACTGTTAGAGAA+CGG - tig0019143:166094-166113 None:intergenic 45.0%
ACTAGACACGGGCATTTAAG+AGG + tig0019143:166196-166215 Msa1370870:CDS 45.0%
GATCACTGAATGTGCTCAGA+AGG + tig0019143:166284-166303 Msa1370870:CDS 45.0%
AACGACATTTGGTACCCAGA+TGG + tig0019143:166315-166334 Msa1370870:CDS 45.0%
ACGACATTTGGTACCCAGAT+GGG + tig0019143:166316-166335 Msa1370870:CDS 45.0%
TGTATTAAGGTCCCCCATCT+GGG - tig0019143:166332-166351 None:intergenic 45.0%
ATGTATTAAGGTCCCCCATC+TGG - tig0019143:166333-166352 None:intergenic 45.0%
AGATGGGGGACCTTAATACA+TGG + tig0019143:166332-166351 Msa1370870:CDS 45.0%
! AACAAGAGCTAGAGCGTTAC+TGG - tig0019143:166467-166486 None:intergenic 45.0%
AAACTTGCCACTACAGGTCT+AGG + tig0019143:166490-166509 Msa1370870:CDS 45.0%
TGGAAGAAGCCCTAGCAAGT+GGG + tig0019143:166108-166127 Msa1370870:CDS 50.0%
! GCTGGAGTGTCAGTGACAAT+CGG + tig0019143:166136-166155 Msa1370870:CDS 50.0%
CGACATTTGGTACCCAGATG+GGG + tig0019143:166317-166336 Msa1370870:CDS 50.0%
GACATTTGGTACCCAGATGG+GGG + tig0019143:166318-166337 Msa1370870:CDS 50.0%
GATGCTTCCTAGACCTGTAG+TGG - tig0019143:166500-166519 None:intergenic 50.0%
GCACCGTTCTCTAACAGTGC+TGG + tig0019143:166088-166107 Msa1370870:CDS 55.0%
CTGGAAGAAGCCCTAGCAAG+TGG + tig0019143:166107-166126 Msa1370870:CDS 55.0%
AAGCCCTAGCAAGTGGGCAA+GGG + tig0019143:166114-166133 Msa1370870:CDS 55.0%
GTGCTAGCGAGCGCGTACAA+AGG - tig0019143:166072-166091 None:intergenic 60.0%
GAAGCCCTAGCAAGTGGGCA+AGG + tig0019143:166113-166132 Msa1370870:CDS 60.0%
! CAGCCCTTGCCCACTTGCTA+GGG - tig0019143:166120-166139 None:intergenic 60.0%
CCAGCCCTTGCCCACTTGCT+AGG - tig0019143:166121-166140 None:intergenic 65.0%
CCTAGCAAGTGGGCAAGGGC+TGG + tig0019143:166118-166137 Msa1370870:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0019143 gene 165938 166531 165938 ID=Msa1370870;Name=Msa1370870
tig0019143 mRNA 165938 166531 165938 ID=Msa1370870-mRNA-1;Parent=Msa1370870;Name=Msa1370870-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|197
tig0019143 exon 165938 166531 165938 ID=Msa1370870-mRNA-1:exon:1786;Parent=Msa1370870-mRNA-1
tig0019143 CDS 165938 166531 165938 ID=Msa1370870-mRNA-1:cds;Parent=Msa1370870-mRNA-1
Gene Sequence

>Msa1370870

ATGGTCAGAAGAATATCACACCTTACCTTTCTGCTTGCATTTATTTTTATTCCATACCTTGCAAGTCAGTTGGTATTTGCAAAAGGGAAAGACAATGTTAGAGAAGCTTGCAAAGTAACTAGGTATCAAAACCTTTGTACGCGCTCGCTAGCACCGTTCTCTAACAGTGCTGGAAGAAGCCCTAGCAAGTGGGCAAGGGCTGGAGTGTCAGTGACAATCGGTGAAGTTAAGAATGTCCAAGCATATCTCGCAAACTTAACTAGACACGGGCATTTAAGAGGAAGAAATAAAGTTGCATTATTAGATTGTGTCGAGACTATTGCAGATGCCCTTGATGAGCTTCATAGATCACTGAATGTGCTCAGAAGGCTAAGTAGAACGACATTTGGTACCCAGATGGGGGACCTTAATACATGGATAAGTGCAGCACTCACAGATCAAGATACTTGCCTTGATGGATTTCAAGGCAAAAACGGTAGGACAATTCAATTGCTGCAAAAGAGAGTTCTCAAAGCCTATTATATAACCAGTAACGCTCTAGCTCTTGTTAATAAACTTGCCACTACAGGTCTAGGAAGCATCTCTGATCCATAG

Protein sequence

>Msa1370870

MVRRISHLTFLLAFIFIPYLASQLVFAKGKDNVREACKVTRYQNLCTRSLAPFSNSAGRSPSKWARAGVSVTIGEVKNVQAYLANLTRHGHLRGRNKVALLDCVETIADALDELHRSLNVLRRLSRTTFGTQMGDLNTWISAALTDQDTCLDGFQGKNGRTIQLLQKRVLKAYYITSNALALVNKLATTGLGSISDP*