AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1455590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1455590 MtrunA17_Chr6g0464801 51.282 78 22 2 65 138 7 72 4.47e-15 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACATTTGCTTCTTCATT+TGG 0.284164 tig0036374:-17542 Msa1455590:intergenic
TTATCAGCACTTTGATAGTT+AGG 0.296935 tig0036374:+18524 Msa1455590:CDS
GTACTAAGATCTGATCTTCT+TGG 0.327969 tig0036374:-18556 Msa1455590:intergenic
GTACTAAGATCTGATCTTCT+TGG 0.327969 tig0036374:-18592 Msa1455590:intergenic
ACATGGATGGTAAAGATAAA+AGG 0.351663 tig0036374:-17582 Msa1455590:intergenic
TCAGATCTTAGTACTGGAAT+AGG 0.426411 tig0036374:+18601 Msa1455590:CDS
GAAACTTTCTTCAAATGTCT+AGG 0.440784 tig0036374:+17626 Msa1455590:CDS
CTTAGTATTGGAAGAGGTCC+AGG 0.443199 tig0036374:+18643 Msa1455590:CDS
AGCAATTAAAGCACAAGAAT+CGG 0.451124 tig0036374:+17479 None:intergenic
AAACTTTCTTCAAATGTCTA+GGG 0.469767 tig0036374:+17627 Msa1455590:CDS
GATGCAGAAGAGAATCTTGA+TGG 0.479422 tig0036374:+17659 Msa1455590:CDS
AGAAGATCAGATCTTAGTAT+TGG 0.495981 tig0036374:+18631 Msa1455590:CDS
AGAAATGCTGAATTAATCCC+TGG 0.508797 tig0036374:-18661 Msa1455590:intergenic
AGAAGATCAGATCTTAGTAC+TGG 0.511957 tig0036374:+18559 Msa1455590:CDS
AGAAGATCAGATCTTAGTAC+TGG 0.511957 tig0036374:+18595 Msa1455590:CDS
AAAGAATGGTCAACGAGTAA+TGG 0.539417 tig0036374:+17707 Msa1455590:CDS
TTTGGAGTAAAGAACATGGA+TGG 0.540921 tig0036374:-17595 Msa1455590:intergenic
TCAGATCTTAGTACTGGAAG+AGG 0.548826 tig0036374:+18565 Msa1455590:CDS
ACTTTGATAGTTAGGAGAGA+TGG 0.558375 tig0036374:+18532 Msa1455590:CDS
AGAAGAGTCAAGAAGAACAA+TGG 0.568313 tig0036374:+17508 Msa1455590:CDS
TCAGATCTTAGTATTGGAAG+AGG 0.585484 tig0036374:+18637 Msa1455590:CDS
AAGCAAATGTTACAAATGAG+AGG 0.594342 tig0036374:+17552 Msa1455590:CDS
GATCTTAGTACTGGAAGAGG+TGG 0.595804 tig0036374:+18568 Msa1455590:CDS
GATCTTAGTACTGGAATAGG+TGG 0.606559 tig0036374:+18604 Msa1455590:CDS
TTAGTATTGGAAGAGGTCCA+GGG 0.652914 tig0036374:+18644 Msa1455590:CDS
TGGATGGTAAAGATAAAAGG+TGG 0.667132 tig0036374:-17579 Msa1455580:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAAAAAAATGATTAATT+TGG + tig0036374:17880-17899 Msa1455590:intron 10.0%
!! TATAAATGTGTTAAAAATAT+GGG - tig0036374:18135-18154 Msa1455590:intergenic 10.0%
!! TTATAAATGTGTTAAAAATA+TGG - tig0036374:18136-18155 Msa1455590:intergenic 10.0%
!! AAGTAAAAAAATGATTAATT+TGG + tig0036374:17880-17899 Msa1455590:intron 10.0%
!! TATAAATGTGTTAAAAATAT+GGG - tig0036374:18135-18154 Msa1455590:intergenic 10.0%
!! TTATAAATGTGTTAAAAATA+TGG - tig0036374:18136-18155 Msa1455590:intergenic 10.0%
!!! AATTAAGAGTTGATATTTTT+AGG + tig0036374:17926-17945 Msa1455590:intron 15.0%
!!! ATTAAGAGTTGATATTTTTA+GGG + tig0036374:17927-17946 Msa1455590:intron 15.0%
!!! AATTAAGAGTTGATATTTTT+AGG + tig0036374:17926-17945 Msa1455590:intron 15.0%
!!! ATTAAGAGTTGATATTTTTA+GGG + tig0036374:17927-17946 Msa1455590:intron 15.0%
!!! AGAAAGTTTCAAGAAATTTT+TGG - tig0036374:17616-17635 Msa1455590:intergenic 20.0%
!!! AGAAAGTTTCAAGAAATTTT+TGG - tig0036374:17616-17635 Msa1455590:intergenic 20.0%
!!! AATTTTTGGAGTAAAGAACA+TGG - tig0036374:17602-17621 Msa1455590:intergenic 25.0%
! AAACTTTCTTCAAATGTCTA+GGG + tig0036374:17627-17646 Msa1455590:CDS 25.0%
!!! AATGTTAACTTGTGTCATTT+TGG + tig0036374:17951-17970 Msa1455590:intron 25.0%
! ACAACTAAATGTTAGTATGT+TGG + tig0036374:18209-18228 Msa1455590:intron 25.0%
!! TTCAGATTAAGTTGTTAATG+AGG + tig0036374:18267-18286 Msa1455590:intron 25.0%
!!! AGTTGTTAATGAGGTTTTTT+TGG + tig0036374:18276-18295 Msa1455590:intron 25.0%
! AGGTTAAACACAAATCATTT+AGG + tig0036374:18403-18422 Msa1455590:intron 25.0%
!! GAAAATCGAAAATCGAATTT+TGG + tig0036374:18425-18444 Msa1455590:intron 25.0%
!! ATATTCTAGAGAAAGATTGT+TGG - tig0036374:18453-18472 Msa1455590:intergenic 25.0%
!!! AATTTTTGGAGTAAAGAACA+TGG - tig0036374:17602-17621 Msa1455590:intergenic 25.0%
! AAACTTTCTTCAAATGTCTA+GGG + tig0036374:17627-17646 Msa1455590:CDS 25.0%
!!! AATGTTAACTTGTGTCATTT+TGG + tig0036374:17951-17970 Msa1455590:intron 25.0%
! ACAACTAAATGTTAGTATGT+TGG + tig0036374:18209-18228 Msa1455590:intron 25.0%
!! TTCAGATTAAGTTGTTAATG+AGG + tig0036374:18267-18286 Msa1455590:intron 25.0%
!!! AGTTGTTAATGAGGTTTTTT+TGG + tig0036374:18276-18295 Msa1455590:intron 25.0%
! AGGTTAAACACAAATCATTT+AGG + tig0036374:18403-18422 Msa1455590:intron 25.0%
!! GAAAATCGAAAATCGAATTT+TGG + tig0036374:18425-18444 Msa1455590:intron 25.0%
!! ATATTCTAGAGAAAGATTGT+TGG - tig0036374:18453-18472 Msa1455590:intergenic 25.0%
GTAACATTTGCTTCTTCATT+TGG - tig0036374:17545-17564 Msa1455590:intergenic 30.0%
AAGCAAATGTTACAAATGAG+AGG + tig0036374:17552-17571 Msa1455590:CDS 30.0%
ACATGGATGGTAAAGATAAA+AGG - tig0036374:17585-17604 Msa1455590:intergenic 30.0%
GAAACTTTCTTCAAATGTCT+AGG + tig0036374:17626-17645 Msa1455590:CDS 30.0%
! ATATGGGTTATGTTAACTTG+TGG - tig0036374:18119-18138 Msa1455590:intergenic 30.0%
! TTAGTATGTTGGTGAATATG+TGG + tig0036374:18220-18239 Msa1455590:intron 30.0%
ACAACTTAATCTGAATGACT+TGG - tig0036374:18262-18281 Msa1455590:intergenic 30.0%
! CTGTATCGTTCCAATTTTAT+CGG + tig0036374:18353-18372 Msa1455590:intron 30.0%
! TTATCAGCACTTTGATAGTT+AGG + tig0036374:18524-18543 Msa1455590:CDS 30.0%
AGAAGATCAGATCTTAGTAT+TGG + tig0036374:18631-18650 Msa1455590:CDS 30.0%
GTAACATTTGCTTCTTCATT+TGG - tig0036374:17545-17564 Msa1455590:intergenic 30.0%
AAGCAAATGTTACAAATGAG+AGG + tig0036374:17552-17571 Msa1455590:CDS 30.0%
ACATGGATGGTAAAGATAAA+AGG - tig0036374:17585-17604 Msa1455590:intergenic 30.0%
GAAACTTTCTTCAAATGTCT+AGG + tig0036374:17626-17645 Msa1455590:CDS 30.0%
! ATATGGGTTATGTTAACTTG+TGG - tig0036374:18119-18138 Msa1455590:intergenic 30.0%
! TTAGTATGTTGGTGAATATG+TGG + tig0036374:18220-18239 Msa1455590:intron 30.0%
ACAACTTAATCTGAATGACT+TGG - tig0036374:18262-18281 Msa1455590:intergenic 30.0%
! CTGTATCGTTCCAATTTTAT+CGG + tig0036374:18353-18372 Msa1455590:intron 30.0%
! TTATCAGCACTTTGATAGTT+AGG + tig0036374:18524-18543 Msa1455590:CDS 30.0%
AGAAGATCAGATCTTAGTAT+TGG + tig0036374:18631-18650 Msa1455590:CDS 30.0%
AGAAGAGTCAAGAAGAACAA+TGG + tig0036374:17508-17527 Msa1455590:CDS 35.0%
TGGATGGTAAAGATAAAAGG+TGG - tig0036374:17582-17601 Msa1455590:intergenic 35.0%
! TTTGGAGTAAAGAACATGGA+TGG - tig0036374:17598-17617 Msa1455590:intergenic 35.0%
CATGTGCAGAACAAAAAGAA+TGG + tig0036374:17693-17712 Msa1455590:CDS 35.0%
AAAGAATGGTCAACGAGTAA+TGG + tig0036374:17707-17726 Msa1455590:CDS 35.0%
GTTTCTTAACTTATGCCCTA+AGG + tig0036374:18093-18112 Msa1455590:intron 35.0%
!! TATGTTAACTTGTGGCCTTA+GGG - tig0036374:18111-18130 Msa1455590:intergenic 35.0%
!! TTATGTTAACTTGTGGCCTT+AGG - tig0036374:18112-18131 Msa1455590:intergenic 35.0%
!! CTGAATGACTTGGTTCTATA+AGG - tig0036374:18252-18271 Msa1455590:intergenic 35.0%
TCATTAACAACTGTCTCTTC+GGG - tig0036374:18384-18403 Msa1455590:intergenic 35.0%
CTCATTAACAACTGTCTCTT+CGG - tig0036374:18385-18404 Msa1455590:intergenic 35.0%
! ACTTTGATAGTTAGGAGAGA+TGG + tig0036374:18532-18551 Msa1455590:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG - tig0036374:18559-18578 Msa1455590:intergenic 35.0%
AGAAGATCAGATCTTAGTAC+TGG + tig0036374:18559-18578 Msa1455590:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG - tig0036374:18559-18578 Msa1455590:intergenic 35.0%
AGAAGATCAGATCTTAGTAC+TGG + tig0036374:18559-18578 Msa1455590:CDS 35.0%
! TCAGATCTTAGTACTGGAAT+AGG + tig0036374:18601-18620 Msa1455590:CDS 35.0%
! TCAGATCTTAGTATTGGAAG+AGG + tig0036374:18637-18656 Msa1455590:CDS 35.0%
AGAAATGCTGAATTAATCCC+TGG - tig0036374:18664-18683 Msa1455590:intergenic 35.0%
AGAAGAGTCAAGAAGAACAA+TGG + tig0036374:17508-17527 Msa1455590:CDS 35.0%
TGGATGGTAAAGATAAAAGG+TGG - tig0036374:17582-17601 Msa1455590:intergenic 35.0%
! TTTGGAGTAAAGAACATGGA+TGG - tig0036374:17598-17617 Msa1455590:intergenic 35.0%
CATGTGCAGAACAAAAAGAA+TGG + tig0036374:17693-17712 Msa1455590:CDS 35.0%
AAAGAATGGTCAACGAGTAA+TGG + tig0036374:17707-17726 Msa1455590:CDS 35.0%
GTTTCTTAACTTATGCCCTA+AGG + tig0036374:18093-18112 Msa1455590:intron 35.0%
!! TATGTTAACTTGTGGCCTTA+GGG - tig0036374:18111-18130 Msa1455590:intergenic 35.0%
!! TTATGTTAACTTGTGGCCTT+AGG - tig0036374:18112-18131 Msa1455590:intergenic 35.0%
!! CTGAATGACTTGGTTCTATA+AGG - tig0036374:18252-18271 Msa1455590:intergenic 35.0%
TCATTAACAACTGTCTCTTC+GGG - tig0036374:18384-18403 Msa1455590:intergenic 35.0%
CTCATTAACAACTGTCTCTT+CGG - tig0036374:18385-18404 Msa1455590:intergenic 35.0%
! ACTTTGATAGTTAGGAGAGA+TGG + tig0036374:18532-18551 Msa1455590:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG - tig0036374:18559-18578 Msa1455590:intergenic 35.0%
AGAAGATCAGATCTTAGTAC+TGG + tig0036374:18559-18578 Msa1455590:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG - tig0036374:18559-18578 Msa1455590:intergenic 35.0%
AGAAGATCAGATCTTAGTAC+TGG + tig0036374:18559-18578 Msa1455590:CDS 35.0%
! TCAGATCTTAGTACTGGAAT+AGG + tig0036374:18601-18620 Msa1455590:CDS 35.0%
! TCAGATCTTAGTATTGGAAG+AGG + tig0036374:18637-18656 Msa1455590:CDS 35.0%
AGAAATGCTGAATTAATCCC+TGG - tig0036374:18664-18683 Msa1455590:intergenic 35.0%
GATGCAGAAGAGAATCTTGA+TGG + tig0036374:17659-17678 Msa1455590:CDS 40.0%
CATTAACAACTGTCTCTTCG+GGG - tig0036374:18383-18402 Msa1455590:intergenic 40.0%
CGAAGAGACAGTTGTTAATG+AGG + tig0036374:18383-18402 Msa1455590:intron 40.0%
! TCAGATCTTAGTACTGGAAG+AGG + tig0036374:18565-18584 Msa1455590:CDS 40.0%
! GATCTTAGTACTGGAATAGG+TGG + tig0036374:18604-18623 Msa1455590:CDS 40.0%
! TTAGTATTGGAAGAGGTCCA+GGG + tig0036374:18644-18663 Msa1455590:CDS 40.0%
GATGCAGAAGAGAATCTTGA+TGG + tig0036374:17659-17678 Msa1455590:CDS 40.0%
CATTAACAACTGTCTCTTCG+GGG - tig0036374:18383-18402 Msa1455590:intergenic 40.0%
CGAAGAGACAGTTGTTAATG+AGG + tig0036374:18383-18402 Msa1455590:intron 40.0%
! TCAGATCTTAGTACTGGAAG+AGG + tig0036374:18565-18584 Msa1455590:CDS 40.0%
! GATCTTAGTACTGGAATAGG+TGG + tig0036374:18604-18623 Msa1455590:CDS 40.0%
! TTAGTATTGGAAGAGGTCCA+GGG + tig0036374:18644-18663 Msa1455590:CDS 40.0%
TCGGGGACATCCGATAAAAT+TGG - tig0036374:18366-18385 Msa1455590:intergenic 45.0%
! GATCTTAGTACTGGAAGAGG+TGG + tig0036374:18568-18587 Msa1455590:CDS 45.0%
! CTTAGTATTGGAAGAGGTCC+AGG + tig0036374:18643-18662 Msa1455590:CDS 45.0%
TCGGGGACATCCGATAAAAT+TGG - tig0036374:18366-18385 Msa1455590:intergenic 45.0%
! GATCTTAGTACTGGAAGAGG+TGG + tig0036374:18568-18587 Msa1455590:CDS 45.0%
! CTTAGTATTGGAAGAGGTCC+AGG + tig0036374:18643-18662 Msa1455590:CDS 45.0%
!!! TTGTTTTTTTTTTTTTATTT+TGG + tig0036374:17815-17834 Msa1455590:intron 5.0%
!!! TGTTTTTTTTTTTTTATTTT+GGG + tig0036374:17816-17835 Msa1455590:intron 5.0%
!!! TAATTAGTTTTTTTTTTTTT+GGG - tig0036374:18174-18193 Msa1455590:intergenic 5.0%
!!! ATAATTAGTTTTTTTTTTTT+TGG - tig0036374:18175-18194 Msa1455590:intergenic 5.0%
!!! TTGTTTTTTTTTTTTTATTT+TGG + tig0036374:17815-17834 Msa1455590:intron 5.0%
!!! TGTTTTTTTTTTTTTATTTT+GGG + tig0036374:17816-17835 Msa1455590:intron 5.0%
!!! TAATTAGTTTTTTTTTTTTT+GGG - tig0036374:18174-18193 Msa1455590:intergenic 5.0%
!!! ATAATTAGTTTTTTTTTTTT+TGG - tig0036374:18175-18194 Msa1455590:intergenic 5.0%
TGTGCATATCATCCTTGCGC+AGG + tig0036374:18313-18332 Msa1455590:intron 50.0%
GTGCATATCATCCTTGCGCA+GGG + tig0036374:18314-18333 Msa1455590:intron 50.0%
TGCATATCATCCTTGCGCAG+GGG + tig0036374:18315-18334 Msa1455590:intron 50.0%
TGTGCATATCATCCTTGCGC+AGG + tig0036374:18313-18332 Msa1455590:intron 50.0%
GTGCATATCATCCTTGCGCA+GGG + tig0036374:18314-18333 Msa1455590:intron 50.0%
TGCATATCATCCTTGCGCAG+GGG + tig0036374:18315-18334 Msa1455590:intron 50.0%
ATTAGCATGACCCCTGCGCA+AGG - tig0036374:18328-18347 Msa1455590:intergenic 55.0%
ATTAGCATGACCCCTGCGCA+AGG - tig0036374:18328-18347 Msa1455590:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0036374 gene 17491 18687 17491 ID=Msa1455590;Name=Msa1455590
tig0036374 mRNA 17491 18687 17491 ID=Msa1455590-mRNA-1;Parent=Msa1455590;Name=Msa1455590-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|1|0|1|1|1|2|0|138
tig0036374 exon 17491 17739 17491 ID=Msa1455590-mRNA-1:exon:20322;Parent=Msa1455590-mRNA-1
tig0036374 exon 18520 18687 18520 ID=Msa1455590-mRNA-1:exon:20323;Parent=Msa1455590-mRNA-1
tig0036374 CDS 17491 17739 17491 ID=Msa1455590-mRNA-1:cds;Parent=Msa1455590-mRNA-1
tig0036374 CDS 18520 18687 18520 ID=Msa1455590-mRNA-1:cds;Parent=Msa1455590-mRNA-1
Gene Sequence

>Msa1455590

ACAAGAATCGGACACAAAGAAGAGTCAAGAAGAACAATGGAAGAAATATCACCAAATGAAGAAGCAAATGTTACAAATGAGAGGAGTGCCACCTTTTATCTTTACCATCCATGTTCTTTACTCCAAAAATTTCTTGAAACTTTCTTCAAATGTCTAGGGTTTGATGCAGATGCAGAAGAGAATCTTGATGGCAAAGTTCTTGCATGTGCAGAACAAAAAGAATGGTCAACGAGTAATGGTCAAAGTTTTCAACTTATCAGCACTTTGATAGTTAGGAGAGATGGACCAAGAAGATCAGATCTTAGTACTGGAAGAGGTGGACCAAGAAGATCAGATCTTAGTACTGGAATAGGTGGACGAAGAAGATCAGATCTTAGTATTGGAAGAGGTCCAGGGATTAATTCAGCATTTCTTTAA

Protein sequence

>Msa1455590

TRIGHKEESRRTMEEISPNEEANVTNERSATFYLYHPCSLLQKFLETFFKCLGFDADAEENLDGKVLACAEQKEWSTSNGQSFQLISTLIVRRDGPRRSDLSTGRGGPRRSDLSTGIGGRRRSDLSIGRGPGINSAFL*