AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0885060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATCAGCACTTTGATAATT+AGG 0.202441 6_1:+39671488 Msa0885060:CDS
GTAACATTTGCTTCTTCATT+TGG 0.284164 6_1:-39670724 None:intergenic
GGAACTTTCTTCATATGTCT+AGG 0.392353 6_1:+39670808 Msa0885060:CDS
GAACTTTCTTCATATGTCTA+GGG 0.429731 6_1:+39670809 Msa0885060:CDS
TCAAGATTCTCTTCTGCATC+AGG 0.434480 6_1:-39670841 None:intergenic
CTTAGTATTGGAAGAGGTCC+AGG 0.443199 6_1:+39671535 Msa0885060:CDS
GATGCAGAAGAGAATCTTGA+TGG 0.462814 6_1:+39670844 Msa0885060:CDS
AGAAGATCAGATCTTAGTAT+TGG 0.495981 6_1:+39671523 Msa0885060:CDS
AGAAATGCTGAATTAATCCC+TGG 0.508797 6_1:-39671553 None:intergenic
TTTGGAGTAAAGAACATGGA+TGG 0.540921 6_1:-39670777 None:intergenic
AAAAGTTCTTACATATACAT+AGG 0.565562 6_1:-39671595 None:intergenic
AGAAGAGTCAAGAAGAACAA+TGG 0.568313 6_1:+39670690 Msa0885060:CDS
GAATGTTCAACGAGTGATGA+TGG 0.575026 6_1:+39670886 Msa0885060:CDS
GACATATGAAGAAAGTTCCA+AGG 0.581756 6_1:-39670804 None:intergenic
TCAGATCTTAGTATTGGAAG+AGG 0.585484 6_1:+39671529 Msa0885060:CDS
AAGCAAATGTTACAAATGAG+AGG 0.594342 6_1:+39670734 Msa0885060:CDS
ACTTTGATAATTAGGAGAGG+TGG 0.649377 6_1:+39671496 Msa0885060:CDS
TTAGTATTGGAAGAGGTCCA+GGG 0.652914 6_1:+39671536 Msa0885060:CDS
AGCACTTTGATAATTAGGAG+AGG 0.668417 6_1:+39671493 Msa0885060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTGTAAGTAAAAAAATT+TGG + chr6_1:39671038-39671057 Msa0885060:intron 15.0%
!!! TGTTCAAAATAGTTTTTTTA+GGG - chr6_1:39671139-39671158 None:intergenic 15.0%
!!! TTGTTCAAAATAGTTTTTTT+AGG - chr6_1:39671140-39671159 None:intergenic 15.0%
!! ATCTTGTAAGTAAAAAAATT+TGG + chr6_1:39671038-39671057 Msa0885060:intron 15.0%
!!! TGTTCAAAATAGTTTTTTTA+GGG - chr6_1:39671139-39671158 None:intergenic 15.0%
!!! TTGTTCAAAATAGTTTTTTT+AGG - chr6_1:39671140-39671159 None:intergenic 15.0%
!!! TTGTTAATGAGGTTTTTTTT+TGG + chr6_1:39671241-39671260 Msa0885060:intron 20.0%
!! AAAAGTTCTTACATATACAT+AGG - chr6_1:39671598-39671617 None:intergenic 20.0%
!!! TTGTTAATGAGGTTTTTTTT+TGG + chr6_1:39671241-39671260 Msa0885060:intron 20.0%
!! AAAAGTTCTTACATATACAT+AGG - chr6_1:39671598-39671617 None:intergenic 20.0%
!!! AATTTTTGGAGTAAAGAACA+TGG - chr6_1:39670784-39670803 None:intergenic 25.0%
! AATACTAAGTGTTAGTATGT+TGG + chr6_1:39671172-39671191 Msa0885060:intron 25.0%
!! TTCAGATTAAGTTGTTAATG+AGG + chr6_1:39671230-39671249 Msa0885060:intron 25.0%
!!! GTTTTTTTTTGGACAATTCA+TGG + chr6_1:39671252-39671271 Msa0885060:intron 25.0%
!! TTGTATCGTTCCAATTTTAT+CGG + chr6_1:39671318-39671337 Msa0885060:intron 25.0%
! AGGTTAAACACAAATCATTT+AGG + chr6_1:39671368-39671387 Msa0885060:intron 25.0%
!! GAAAATCCAAAATCGAATTT+TGG + chr6_1:39671390-39671409 Msa0885060:intron 25.0%
!! ATATTCTAGAGAAAGATTGT+TGG - chr6_1:39671418-39671437 None:intergenic 25.0%
!! TTATCAGCACTTTGATAATT+AGG + chr6_1:39671488-39671507 Msa0885060:CDS 25.0%
!!! AATTTTTGGAGTAAAGAACA+TGG - chr6_1:39670784-39670803 None:intergenic 25.0%
! AATACTAAGTGTTAGTATGT+TGG + chr6_1:39671172-39671191 Msa0885060:intron 25.0%
!! TTCAGATTAAGTTGTTAATG+AGG + chr6_1:39671230-39671249 Msa0885060:intron 25.0%
!!! GTTTTTTTTTGGACAATTCA+TGG + chr6_1:39671252-39671271 Msa0885060:intron 25.0%
!! TTGTATCGTTCCAATTTTAT+CGG + chr6_1:39671318-39671337 Msa0885060:intron 25.0%
! AGGTTAAACACAAATCATTT+AGG + chr6_1:39671368-39671387 Msa0885060:intron 25.0%
!! GAAAATCCAAAATCGAATTT+TGG + chr6_1:39671390-39671409 Msa0885060:intron 25.0%
!! ATATTCTAGAGAAAGATTGT+TGG - chr6_1:39671418-39671437 None:intergenic 25.0%
!! TTATCAGCACTTTGATAATT+AGG + chr6_1:39671488-39671507 Msa0885060:CDS 25.0%
GTAACATTTGCTTCTTCATT+TGG - chr6_1:39670727-39670746 None:intergenic 30.0%
AAGCAAATGTTACAAATGAG+AGG + chr6_1:39670734-39670753 Msa0885060:CDS 30.0%
TGGATGGTAAAGACAAAAAT+TGG - chr6_1:39670764-39670783 None:intergenic 30.0%
TCTTTACTCCAAAAATTCCT+TGG + chr6_1:39670787-39670806 Msa0885060:CDS 30.0%
! AGAAAGTTCCAAGGAATTTT+TGG - chr6_1:39670798-39670817 None:intergenic 30.0%
GAACTTTCTTCATATGTCTA+GGG + chr6_1:39670809-39670828 Msa0885060:CDS 30.0%
ACAACTTAATCTGAATGACT+TGG - chr6_1:39671225-39671244 None:intergenic 30.0%
! TTGGAACCAAAATTCGATTT+TGG - chr6_1:39671399-39671418 None:intergenic 30.0%
AGAAGATCAGATCTTAGTAT+TGG + chr6_1:39671523-39671542 Msa0885060:CDS 30.0%
GTAACATTTGCTTCTTCATT+TGG - chr6_1:39670727-39670746 None:intergenic 30.0%
AAGCAAATGTTACAAATGAG+AGG + chr6_1:39670734-39670753 Msa0885060:CDS 30.0%
TGGATGGTAAAGACAAAAAT+TGG - chr6_1:39670764-39670783 None:intergenic 30.0%
TCTTTACTCCAAAAATTCCT+TGG + chr6_1:39670787-39670806 Msa0885060:CDS 30.0%
! AGAAAGTTCCAAGGAATTTT+TGG - chr6_1:39670798-39670817 None:intergenic 30.0%
GAACTTTCTTCATATGTCTA+GGG + chr6_1:39670809-39670828 Msa0885060:CDS 30.0%
ACAACTTAATCTGAATGACT+TGG - chr6_1:39671225-39671244 None:intergenic 30.0%
! TTGGAACCAAAATTCGATTT+TGG - chr6_1:39671399-39671418 None:intergenic 30.0%
AGAAGATCAGATCTTAGTAT+TGG + chr6_1:39671523-39671542 Msa0885060:CDS 30.0%
AGAAGAGTCAAGAAGAACAA+TGG + chr6_1:39670690-39670709 Msa0885060:CDS 35.0%
! TTTGGAGTAAAGAACATGGA+TGG - chr6_1:39670780-39670799 None:intergenic 35.0%
GACATATGAAGAAAGTTCCA+AGG - chr6_1:39670807-39670826 None:intergenic 35.0%
GGAACTTTCTTCATATGTCT+AGG + chr6_1:39670808-39670827 Msa0885060:CDS 35.0%
! GTGTTAGTATGTTGGTGAAT+TGG + chr6_1:39671180-39671199 Msa0885060:intron 35.0%
!! CTGAATGACTTGGTTCTATA+AGG - chr6_1:39671215-39671234 None:intergenic 35.0%
TCATTAACAACTGTCCCTTT+GGG - chr6_1:39671349-39671368 None:intergenic 35.0%
! AGCACTTTGATAATTAGGAG+AGG + chr6_1:39671493-39671512 Msa0885060:CDS 35.0%
! ACTTTGATAATTAGGAGAGG+TGG + chr6_1:39671496-39671515 Msa0885060:CDS 35.0%
! TCAGATCTTAGTATTGGAAG+AGG + chr6_1:39671529-39671548 Msa0885060:CDS 35.0%
AGAAATGCTGAATTAATCCC+TGG - chr6_1:39671556-39671575 None:intergenic 35.0%
AGAAGAGTCAAGAAGAACAA+TGG + chr6_1:39670690-39670709 Msa0885060:CDS 35.0%
! TTTGGAGTAAAGAACATGGA+TGG - chr6_1:39670780-39670799 None:intergenic 35.0%
GACATATGAAGAAAGTTCCA+AGG - chr6_1:39670807-39670826 None:intergenic 35.0%
GGAACTTTCTTCATATGTCT+AGG + chr6_1:39670808-39670827 Msa0885060:CDS 35.0%
! GTGTTAGTATGTTGGTGAAT+TGG + chr6_1:39671180-39671199 Msa0885060:intron 35.0%
!! CTGAATGACTTGGTTCTATA+AGG - chr6_1:39671215-39671234 None:intergenic 35.0%
TCATTAACAACTGTCCCTTT+GGG - chr6_1:39671349-39671368 None:intergenic 35.0%
! AGCACTTTGATAATTAGGAG+AGG + chr6_1:39671493-39671512 Msa0885060:CDS 35.0%
! ACTTTGATAATTAGGAGAGG+TGG + chr6_1:39671496-39671515 Msa0885060:CDS 35.0%
! TCAGATCTTAGTATTGGAAG+AGG + chr6_1:39671529-39671548 Msa0885060:CDS 35.0%
AGAAATGCTGAATTAATCCC+TGG - chr6_1:39671556-39671575 None:intergenic 35.0%
TCAAGATTCTCTTCTGCATC+AGG - chr6_1:39670844-39670863 None:intergenic 40.0%
GATGCAGAAGAGAATCTTGA+TGG + chr6_1:39670844-39670863 Msa0885060:CDS 40.0%
GAATGTTCAACGAGTGATGA+TGG + chr6_1:39670886-39670905 Msa0885060:CDS 40.0%
TTGGGGACATCCGATAAAAT+TGG - chr6_1:39671331-39671350 None:intergenic 40.0%
! ATTTTATCGGATGTCCCCAA+AGG + chr6_1:39671331-39671350 Msa0885060:intron 40.0%
! TTTTATCGGATGTCCCCAAA+GGG + chr6_1:39671332-39671351 Msa0885060:intron 40.0%
CATTAACAACTGTCCCTTTG+GGG - chr6_1:39671348-39671367 None:intergenic 40.0%
CTCATTAACAACTGTCCCTT+TGG - chr6_1:39671350-39671369 None:intergenic 40.0%
CAAAGGGACAGTTGTTAATG+AGG + chr6_1:39671348-39671367 Msa0885060:intron 40.0%
! TTAGTATTGGAAGAGGTCCA+GGG + chr6_1:39671536-39671555 Msa0885060:CDS 40.0%
TCAAGATTCTCTTCTGCATC+AGG - chr6_1:39670844-39670863 None:intergenic 40.0%
GATGCAGAAGAGAATCTTGA+TGG + chr6_1:39670844-39670863 Msa0885060:CDS 40.0%
GAATGTTCAACGAGTGATGA+TGG + chr6_1:39670886-39670905 Msa0885060:CDS 40.0%
TTGGGGACATCCGATAAAAT+TGG - chr6_1:39671331-39671350 None:intergenic 40.0%
! ATTTTATCGGATGTCCCCAA+AGG + chr6_1:39671331-39671350 Msa0885060:intron 40.0%
! TTTTATCGGATGTCCCCAAA+GGG + chr6_1:39671332-39671351 Msa0885060:intron 40.0%
CATTAACAACTGTCCCTTTG+GGG - chr6_1:39671348-39671367 None:intergenic 40.0%
CTCATTAACAACTGTCCCTT+TGG - chr6_1:39671350-39671369 None:intergenic 40.0%
CAAAGGGACAGTTGTTAATG+AGG + chr6_1:39671348-39671367 Msa0885060:intron 40.0%
! TTAGTATTGGAAGAGGTCCA+GGG + chr6_1:39671536-39671555 Msa0885060:CDS 40.0%
! CTTAGTATTGGAAGAGGTCC+AGG + chr6_1:39671535-39671554 Msa0885060:CDS 45.0%
! CTTAGTATTGGAAGAGGTCC+AGG + chr6_1:39671535-39671554 Msa0885060:CDS 45.0%
!!! TAAAGTTTTTTTTTTTTTTT+TGG + chr6_1:39670980-39670999 Msa0885060:intron 5.0%
!!! AAAGTTTTTTTTTTTTTTTT+GGG + chr6_1:39670981-39671000 Msa0885060:intron 5.0%
!!! TAAAGTTTTTTTTTTTTTTT+TGG + chr6_1:39670980-39670999 Msa0885060:intron 5.0%
!!! AAAGTTTTTTTTTTTTTTTT+GGG + chr6_1:39670981-39671000 Msa0885060:intron 5.0%
ATTAGCATGACCCCTGCGCA+AGG - chr6_1:39671293-39671312 None:intergenic 55.0%
ATTAGCATGACCCCTGCGCA+AGG - chr6_1:39671293-39671312 None:intergenic 55.0%
TGTGCGTGTCATCCTTGCGC+AGG + chr6_1:39671278-39671297 Msa0885060:intron 60.0%
GTGCGTGTCATCCTTGCGCA+GGG + chr6_1:39671279-39671298 Msa0885060:intron 60.0%
TGCGTGTCATCCTTGCGCAG+GGG + chr6_1:39671280-39671299 Msa0885060:intron 60.0%
TGTGCGTGTCATCCTTGCGC+AGG + chr6_1:39671278-39671297 Msa0885060:intron 60.0%
GTGCGTGTCATCCTTGCGCA+GGG + chr6_1:39671279-39671298 Msa0885060:intron 60.0%
TGCGTGTCATCCTTGCGCAG+GGG + chr6_1:39671280-39671299 Msa0885060:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 39670679 39671637 39670679 ID=Msa0885060;Name=Msa0885060
chr6_1 mRNA 39670679 39671637 39670679 ID=Msa0885060-mRNA-1;Parent=Msa0885060;Name=Msa0885060-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0.5|1|1|1|2|58|111
chr6_1 exon 39670679 39670918 39670679 ID=Msa0885060-mRNA-1:exon:9069;Parent=Msa0885060-mRNA-1
chr6_1 exon 39671484 39671637 39671484 ID=Msa0885060-mRNA-1:exon:9070;Parent=Msa0885060-mRNA-1
chr6_1 CDS 39670679 39670918 39670679 ID=Msa0885060-mRNA-1:cds;Parent=Msa0885060-mRNA-1
chr6_1 CDS 39671484 39671579 39671484 ID=Msa0885060-mRNA-1:cds;Parent=Msa0885060-mRNA-1
chr6_1 three_prime_UTR 39671580 39671637 39671580 ID=Msa0885060-mRNA-1:three_prime_utr;Parent=Msa0885060-mRNA-1
Gene Sequence

>Msa0885060

ATGAGACACAAAGAAGAGTCAAGAAGAACAATGGAAGAAATATCACCAAATGAAGAAGCAAATGTTACAAATGAGAGGAGTGCCAATTTTTGTCTTTACCATCCATGTTCTTTACTCCAAAAATTCCTTGGAACTTTCTTCATATGTCTAGGGTTTCAAATTCCTGATGCAGAAGAGAATCTTGATGGCAAAGTTCTTGCATGTGAAGAATGTTCAACGAGTGATGATGGTCAAAGTTTTCAACTTATCAGCACTTTGATAATTAGGAGAGGTGGACGAAGAAGATCAGATCTTAGTATTGGAAGAGGTCCAGGGATTAATTCAGCATTTCTTTAA

Protein sequence

>Msa0885060

MRHKEESRRTMEEISPNEEANVTNERSANFCLYHPCSLLQKFLGTFFICLGFQIPDAEENLDGKVLACEECSTSDDGQSFQLISTLIIRRGGRRRSDLSIGRGPGINSAFL*