AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0444010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0444010 MtrunA17_Chr3g0083761 87.225 227 11 2 1 209 238 464 1.93e-139 398
Msa0444010 MtrunA17_Chr7g0219881 58.296 223 73 4 1 208 245 462 2.68e-80 247
Msa0444010 MtrunA17_Chr2g0323511 38.326 227 116 6 2 209 264 485 5.81e-41 145
Msa0444010 MtrunA17_Chr2g0323471 40.741 216 113 6 2 209 272 480 4.37e-38 137
Msa0444010 MtrunA17_Chr8g0339641 39.381 226 118 6 2 209 251 475 6.74e-37 134
Msa0444010 MtrunA17_Chr2g0323501 32.420 219 133 6 2 207 260 476 2.44e-28 110
Msa0444010 MtrunA17_Chr3g0087901 33.032 221 135 5 1 209 248 467 3.91e-28 110
Msa0444010 MtrunA17_Chr7g0219301 35.714 210 121 7 3 202 672 877 5.28e-28 110
Msa0444010 MtrunA17_Chr7g0219301 33.333 210 126 6 3 202 249 454 7.60e-28 110
Msa0444010 MtrunA17_Chr2g0323481 34.703 219 126 7 2 207 248 462 1.10e-27 108
Msa0444010 MtrunA17_Chr7g0219331 32.857 210 127 6 3 202 342 547 9.25e-27 106
Msa0444010 MtrunA17_Chr2g0323421 33.929 224 127 8 2 209 294 512 1.12e-26 106
Msa0444010 MtrunA17_Chr7g0219241 32.512 203 122 4 2 190 256 457 9.95e-24 97.8
Msa0444010 MtrunA17_Chr7g0219121 33.816 207 117 7 2 191 259 462 1.30e-23 97.4
Msa0444010 MtrunA17_Chr7g0219181 30.263 228 119 7 2 202 257 471 3.82e-23 96.3
Msa0444010 MtrunA17_Chr6g0454761 31.754 211 133 6 6 209 739 945 4.72e-23 96.3
Msa0444010 MtrunA17_Chr6g0454761 29.646 226 125 6 2 209 248 457 4.54e-20 87.8
Msa0444010 MtrunA17_Chr7g0219291 31.193 218 128 6 2 202 599 811 1.58e-21 92.0
Msa0444010 MtrunA17_Chr7g0219291 32.020 203 122 6 2 190 112 312 1.30e-20 89.4
Msa0444010 MtrunA17_Chr6g0455341 29.302 215 139 4 2 209 255 463 1.68e-21 91.7
Msa0444010 MtrunA17_Chr3g0124341 31.455 213 125 7 12 207 265 473 3.63e-21 90.5
Msa0444010 MtrunA17_Chr6g0455361 29.630 216 138 6 1 209 252 460 5.40e-20 87.0
Msa0444010 MtrunA17_Chr7g0219101 29.911 224 135 7 2 208 210 428 2.22e-19 85.5
Msa0444010 MtrunA17_Chr7g0219221 36.879 141 79 3 59 190 3 142 4.43e-18 77.8
Msa0444010 MtrunA17_Chr7g0219311 33.562 146 87 6 52 189 2 145 3.92e-17 75.1
Msa0444010 MtrunA17_Chr7g0219341 31.973 147 91 5 64 202 1 146 2.24e-16 73.2
Msa0444010 MtrunA17_Chr7g0219201 30.159 189 122 3 20 199 8 195 3.41e-15 71.2
Msa0444010 MtrunA17_Chr2g0323491 37.333 75 47 0 135 209 6 80 3.55e-13 62.8
Msa0444010 MtrunA17_Chr3g0092001 33.065 124 73 4 92 209 1 120 4.49e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0444010 AT1G03940.1 29.386 228 131 6 1 207 247 465 9.35e-28 109
Msa0444010 AT3G29635.1 31.731 208 127 7 1 198 244 446 2.52e-22 94.4
Msa0444010 AT3G29670.1 28.502 207 125 8 1 195 243 438 5.93e-21 90.1
Msa0444010 AT5G39090.1 28.837 215 141 6 1 207 238 448 1.02e-18 84.0
Msa0444010 AT3G29680.1 30.435 207 129 9 1 198 239 439 4.85e-18 82.0
Msa0444010 AT3G29590.1 28.571 217 139 5 1 206 237 448 2.28e-17 80.1
Msa0444010 AT5G61160.1 29.143 175 114 6 32 198 270 442 5.17e-17 79.0
Msa0444010 AT5G39050.1 25.248 202 142 5 14 208 270 469 1.41e-16 77.8
Msa0444010 AT5G39080.1 25.463 216 149 7 1 206 248 461 5.93e-16 75.9

Find 30 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATTTCAACAAATTATTT+TGG 0.164463 3_3:-16577243 Msa0444010:CDS
TTCTATTGGAATAGAAATTA+AGG 0.170988 3_3:-16577142 Msa0444010:CDS
TTGTTGCATGTTCTTTGTTT+TGG 0.232091 3_3:-16577389 Msa0444010:CDS
TTTGAAGATAATTACGGTTT+TGG 0.233969 3_3:-16577294 Msa0444010:intron
GTTTGATTGATCCACTAAAA+AGG 0.238232 3_3:-16577119 Msa0444010:CDS
TGTGAAGCAGTTAATTCTAT+TGG 0.291099 3_3:-16577156 Msa0444010:CDS
TATCTCTAAGTTGAGCTTCT+AGG 0.296611 3_3:+16576844 None:intergenic
ACTTCCAAAGAGGAAGTTAT+TGG 0.300492 3_3:-16577193 Msa0444010:CDS
TCTCCCAATAACTTCCTCTT+TGG 0.322116 3_3:+16577189 None:intergenic
TGAAGAATTTCACTCAAAAC+TGG 0.343769 3_3:+16576975 None:intergenic
AATGTGTATGAAACTAATTT+TGG 0.344845 3_3:-16577008 Msa0444010:CDS
TCTATTGGAATAGAAATTAA+GGG 0.350626 3_3:-16577141 Msa0444010:CDS
TGAAGTTGGGATGGTGTTAG+AGG 0.372013 3_3:-16576895 Msa0444010:CDS
AATTTGTTGAAATCTCAAAA+TGG 0.424170 3_3:+16577250 None:intergenic
GAAGATCATGGCATTGAAGT+TGG 0.433604 3_3:-16576909 Msa0444010:CDS
CTTCCAAAGAGGAAGTTATT+GGG 0.437547 3_3:-16577192 Msa0444010:CDS
TTTACAACGTGCTAGGGACT+TGG 0.441330 3_3:-16577078 Msa0444010:intron
AGGAAGTTATTGGGAGAGAA+TGG 0.458911 3_3:-16577183 Msa0444010:CDS
GTTTCATACACATTAAACTT+TGG 0.462228 3_3:+16577017 None:intergenic
TTACAACGTGCTAGGGACTT+GGG 0.476923 3_3:-16577077 Msa0444010:intron
AAGATCATGGCATTGAAGTT+GGG 0.519789 3_3:-16576908 Msa0444010:CDS
GATGGTGTTAGAGGCAGCTC+AGG 0.527956 3_3:-16576886 Msa0444010:CDS
TTGTTTAGGCTCTTGTGTTG+CGG 0.539510 3_3:-16577217 Msa0444010:CDS
TTTGAGTGAAATTCTTCATG+TGG 0.546331 3_3:-16576970 Msa0444010:CDS
AGTTCTAAAGATGAAGATCA+TGG 0.559685 3_3:-16576921 Msa0444010:CDS
TATATTAACACATCATCAAG+CGG 0.578546 3_3:-16577484 Msa0444010:CDS
TACAACGTGCTAGGGACTTG+GGG 0.583528 3_3:-16577076 Msa0444010:intron
CAAAGAACATGCAACAACAA+AGG 0.618163 3_3:+16577395 None:intergenic
GTGTTGCGGCACTTCCAAAG+AGG 0.623285 3_3:-16577203 Msa0444010:CDS
TCATGGCATTGAAGTTGGGA+TGG 0.653838 3_3:-16576904 Msa0444010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATTTTGAAGATAATTA+CGG - chr3_3:16577043-16577062 Msa0444010:CDS 15.0%
!!! AATTATTTTGGAAATTGTTT+AGG - chr3_3:16577112-16577131 Msa0444010:CDS 15.0%
!! GTTACAAAAAGTAAAACTAT+TGG - chr3_3:16576902-16576921 Msa0444010:CDS 20.0%
!!! AATTTGTTGAAATCTCAAAA+TGG + chr3_3:16577096-16577115 None:intergenic 20.0%
!!! GAGATTTCAACAAATTATTT+TGG - chr3_3:16577100-16577119 Msa0444010:intron 20.0%
!! TTCTATTGGAATAGAAATTA+AGG - chr3_3:16577201-16577220 Msa0444010:CDS 20.0%
!! TCTATTGGAATAGAAATTAA+GGG - chr3_3:16577202-16577221 Msa0444010:CDS 20.0%
!!! AATGTGTATGAAACTAATTT+TGG - chr3_3:16577335-16577354 Msa0444010:intron 20.0%
! TATATTAACACATCATCAAG+CGG - chr3_3:16576859-16576878 Msa0444010:CDS 25.0%
! AAGCGGAAAAATTGAAAAAA+TGG - chr3_3:16576876-16576895 Msa0444010:CDS 25.0%
!! TTTGAAGATAATTACGGTTT+TGG - chr3_3:16577049-16577068 Msa0444010:CDS 25.0%
!!! TTACGGTTTTGGATTTTTAA+TGG - chr3_3:16577060-16577079 Msa0444010:CDS 25.0%
! TTTGATTGATCCACTAAAAA+GGG - chr3_3:16577225-16577244 Msa0444010:CDS 25.0%
! GTTTCATACACATTAAACTT+TGG + chr3_3:16577329-16577348 None:intergenic 25.0%
!! TGTATGAAACTAATTTTGGA+TGG - chr3_3:16577339-16577358 Msa0444010:intron 25.0%
!! GTATGAAACTAATTTTGGAT+GGG - chr3_3:16577340-16577359 Msa0444010:intron 25.0%
!! TATGAAACTAATTTTGGATG+GGG - chr3_3:16577341-16577360 Msa0444010:intron 25.0%
!! TTGTTGCATGTTCTTTGTTT+TGG - chr3_3:16576954-16576973 Msa0444010:CDS 30.0%
!!! TGTTGCATGTTCTTTGTTTT+GGG - chr3_3:16576955-16576974 Msa0444010:CDS 30.0%
!!! TTCTTTGTTTTGGGTATGTA+AGG - chr3_3:16576964-16576983 Msa0444010:CDS 30.0%
TGTGAAGCAGTTAATTCTAT+TGG - chr3_3:16577187-16577206 Msa0444010:CDS 30.0%
GTTTGATTGATCCACTAAAA+AGG - chr3_3:16577224-16577243 Msa0444010:CDS 30.0%
!!! TATGTTTTTTACAACGTGCT+AGG - chr3_3:16577258-16577277 Msa0444010:CDS 30.0%
!!! ATGTTTTTTACAACGTGCTA+GGG - chr3_3:16577259-16577278 Msa0444010:CDS 30.0%
! ATGAAACTAATTTTGGATGG+GGG - chr3_3:16577342-16577361 Msa0444010:intron 30.0%
TGAAGAATTTCACTCAAAAC+TGG + chr3_3:16577371-16577390 None:intergenic 30.0%
TTTGAGTGAAATTCTTCATG+TGG - chr3_3:16577373-16577392 Msa0444010:CDS 30.0%
!! AGTTCTAAAGATGAAGATCA+TGG - chr3_3:16577422-16577441 Msa0444010:CDS 30.0%
CAAAGAACATGCAACAACAA+AGG + chr3_3:16576951-16576970 None:intergenic 35.0%
ACTTCCAAAGAGGAAGTTAT+TGG - chr3_3:16577150-16577169 Msa0444010:CDS 35.0%
CTTCCAAAGAGGAAGTTATT+GGG - chr3_3:16577151-16577170 Msa0444010:CDS 35.0%
! AGATCTTCAACCCTTTTTAG+TGG + chr3_3:16577238-16577257 None:intergenic 35.0%
! AAGATCATGGCATTGAAGTT+GGG - chr3_3:16577435-16577454 Msa0444010:CDS 35.0%
! TATCTCTAAGTTGAGCTTCT+AGG + chr3_3:16577502-16577521 None:intergenic 35.0%
TTGTTTAGGCTCTTGTGTTG+CGG - chr3_3:16577126-16577145 Msa0444010:CDS 40.0%
TCTCCCAATAACTTCCTCTT+TGG + chr3_3:16577157-16577176 None:intergenic 40.0%
AGGAAGTTATTGGGAGAGAA+TGG - chr3_3:16577160-16577179 Msa0444010:CDS 40.0%
! GAAGATCATGGCATTGAAGT+TGG - chr3_3:16577434-16577453 Msa0444010:CDS 40.0%
TTTACAACGTGCTAGGGACT+TGG - chr3_3:16577265-16577284 Msa0444010:CDS 45.0%
TTACAACGTGCTAGGGACTT+GGG - chr3_3:16577266-16577285 Msa0444010:CDS 45.0%
! TCATGGCATTGAAGTTGGGA+TGG - chr3_3:16577439-16577458 Msa0444010:CDS 45.0%
! TGAAGTTGGGATGGTGTTAG+AGG - chr3_3:16577448-16577467 Msa0444010:CDS 45.0%
TACAACGTGCTAGGGACTTG+GGG - chr3_3:16577267-16577286 Msa0444010:CDS 50.0%
GTGTTGCGGCACTTCCAAAG+AGG - chr3_3:16577140-16577159 Msa0444010:CDS 55.0%
! GATGGTGTTAGAGGCAGCTC+AGG - chr3_3:16577457-16577476 Msa0444010:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 16576842 16577523 16576842 ID=Msa0444010;Name=Msa0444010
chr3_3 mRNA 16576842 16577523 16576842 ID=Msa0444010-mRNA-1;Parent=Msa0444010;Name=Msa0444010-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|0|0|3|0|209
chr3_3 exon 16577346 16577523 16577346 ID=Msa0444010-mRNA-1:exon:3465;Parent=Msa0444010-mRNA-1
chr3_3 exon 16577105 16577309 16577105 ID=Msa0444010-mRNA-1:exon:3464;Parent=Msa0444010-mRNA-1
chr3_3 exon 16576842 16577085 16576842 ID=Msa0444010-mRNA-1:exon:3463;Parent=Msa0444010-mRNA-1
chr3_3 CDS 16577346 16577523 16577346 ID=Msa0444010-mRNA-1:cds;Parent=Msa0444010-mRNA-1
chr3_3 CDS 16577105 16577309 16577105 ID=Msa0444010-mRNA-1:cds;Parent=Msa0444010-mRNA-1
chr3_3 CDS 16576842 16577085 16576842 ID=Msa0444010-mRNA-1:cds;Parent=Msa0444010-mRNA-1
Gene Sequence

>Msa0444010

ATGGTTCGTCATAGATTTATATTAACACATCATCAAGCGGAAAAATTGAAAAAATGGATTGTTACAAAAAGTAAAACTATTGGTCTTGAAACACTTCATTTGTCAACCTTTGTTGTTGCATGTTCTTTGTTTTGGGTATGTAAGGTAAAAACACAATCACAACATGACACAAACAAAAATAATTACGGTTTTGGATTTTTAATGGATTTGCGAGACCATTTTGAGATTTCAACAAATTATTTTGGAAATTGTTTAGGCTCTTGTGTTGCGGCACTTCCAAAGAGGAAGTTATTGGGAGAGAATGGTATATGTGAAGCAGTTAATTCTATTGGAATAGAAATTAAGGGTTTGATTGATCCACTAAAAAGGGTTGAAGATCTTATGGACTTGGGGATTAAGTTTCAAAATATAACTTCTGTTGCAGCATCACCAAAGTTTAATGTGTATGAAACTAATTTTGGATGGGGGAAGCCAGTTTTGAGTGAAATTCTTCATGTGGAGAACTCAAGTACATTTTGTCTTTCTAGTTCTAAAGATGAAGATCATGGCATTGAAGTTGGGATGGTGTTAGAGGCAGCTCAGGTGAAGAAATTTAGTGATGTCCTAGAAGCTCAACTTAGAGATATT

Protein sequence

>Msa0444010

MVRHRFILTHHQAEKLKKWIVTKSKTIGLETLHLSTFVVACSLFWVCKVKTQSQHDTNKNNYGFGFLMDLRDHFEISTNYFGNCLGSCVAALPKRKLLGENGICEAVNSIGIEIKGLIDPLKRVEDLMDLGIKFQNITSVAASPKFNVYETNFGWGKPVLSEILHVENSSTFCLSSSKDEDHGIEVGMVLEAAQVKKFSDVLEAQLRDI