Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007048.t1 | GAU47991.1 | 72.9 | 229 | 53 | 1 | 1 | 229 | 129 | 348 | 6.70E-89 | 337 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007048.t1 | Q84WU2 | 40.3 | 72 | 42 | 1 | 4 | 75 | 112 | 182 | 3.8e-08 | 60.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007048.t1 | A0A2Z6NUX7 | 72.9 | 229 | 53 | 1 | 1 | 229 | 129 | 348 | 4.8e-89 | 337.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007048.t1 | MTR_7g107160 | 77.391 | 230 | 22 | 3 | 1 | 229 | 193 | 393 | 4.17e-109 | 321 |
| MS.gene007048.t1 | MTR_7g107160 | 49.550 | 111 | 55 | 1 | 1 | 111 | 1 | 110 | 6.54e-27 | 107 |
| MS.gene007048.t1 | MTR_7g107200 | 47.059 | 255 | 105 | 3 | 3 | 228 | 72 | 325 | 3.69e-69 | 217 |
| MS.gene007048.t1 | MTR_1g042160 | 42.358 | 229 | 95 | 5 | 1 | 229 | 69 | 260 | 7.69e-50 | 165 |
| MS.gene007048.t1 | MTR_8g011730 | 37.154 | 253 | 126 | 6 | 2 | 229 | 72 | 316 | 1.55e-48 | 163 |
| MS.gene007048.t1 | MTR_7g107170 | 59.091 | 110 | 37 | 1 | 120 | 229 | 114 | 215 | 3.08e-36 | 129 |
| MS.gene007048.t1 | MTR_7g107170 | 81.250 | 48 | 9 | 0 | 182 | 229 | 1 | 48 | 2.16e-20 | 87.0 |
| MS.gene007048.t1 | MTR_2g034780 | 48.544 | 103 | 50 | 1 | 127 | 229 | 119 | 218 | 1.23e-25 | 101 |
| MS.gene007048.t1 | MTR_5g091450 | 37.500 | 72 | 44 | 1 | 1 | 72 | 88 | 158 | 3.00e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007048.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 110 | 183 | 1.26e-11 | 64.3 |
| MS.gene007048.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 110 | 183 | 1.27e-11 | 64.3 |
| MS.gene007048.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 1.27e-11 | 64.3 |
| MS.gene007048.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 4.77e-11 | 62.4 |
| MS.gene007048.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 4.77e-11 | 62.4 |
| MS.gene007048.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 108 | 181 | 5.05e-11 | 62.4 |
| MS.gene007048.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 108 | 181 | 5.05e-11 | 62.4 |
Find 43 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAAGAAGTTGTAAGTTTA+GGG | 0.193564 | 7.3:+6564989 | MS.gene007048:CDS |
| AGAAAAGAAGTTGTAAGTTT+AGG | 0.206519 | 7.3:+6564988 | MS.gene007048:CDS |
| TGGCTGGGGTATTGGATCTT+TGG | 0.242019 | 7.3:+6564647 | MS.gene007048:CDS |
| GGAATGGGCGTTCACTGCTT+TGG | 0.299493 | 7.3:+6565010 | MS.gene007048:CDS |
| CTTATGGATTTCAAGGGTTT+AGG | 0.322047 | 7.3:+6564891 | MS.gene007048:CDS |
| GCAAAATCCAGGTGAACTTA+TGG | 0.322911 | 7.3:+6564875 | MS.gene007048:CDS |
| GTAAAAGATGTTTGTATTGT+TGG | 0.326070 | 7.3:+6564711 | MS.gene007048:CDS |
| AGCTCCAATGGCTGGGGTAT+TGG | 0.327929 | 7.3:+6564639 | MS.gene007048:CDS |
| GAATGGGCGTTCACTGCTTT+GGG | 0.331310 | 7.3:+6565011 | MS.gene007048:CDS |
| TAGAGGTCAAACTGAAAATC+TGG | 0.352353 | 7.3:+6564812 | MS.gene007048:CDS |
| TGGGAGGAACTTCAGCCATT+TGG | 0.359556 | 7.3:+6565110 | MS.gene007048:CDS |
| TCACTGCAAGCTCCAATGGC+TGG | 0.398816 | 7.3:+6564631 | MS.gene007048:CDS |
| CACTGCAAGCTCCAATGGCT+GGG | 0.419346 | 7.3:+6564632 | MS.gene007048:CDS |
| TATGAATGACTTATCTTGTA+AGG | 0.428376 | 7.3:+6565073 | MS.gene007048:CDS |
| CCAGGAACTTCTTCTAGCAT+TGG | 0.429065 | 7.3:-6564852 | None:intergenic |
| AGGTGAACTTATGGATTTCA+AGG | 0.444860 | 7.3:+6564884 | MS.gene007048:CDS |
| GTTCTTCATGATCCTCATAG+TGG | 0.455915 | 7.3:+6564681 | MS.gene007048:CDS |
| CCAATGCTAGAAGAAGTTCC+TGG | 0.456723 | 7.3:+6564852 | MS.gene007048:CDS |
| ATCTGGAAGCTGAAGTCATA+AGG | 0.473546 | 7.3:+6564829 | MS.gene007048:CDS |
| AAGTTGTAAGTTTAGGGAAT+GGG | 0.480762 | 7.3:+6564995 | MS.gene007048:CDS |
| GAAGTTGTAAGTTTAGGGAA+TGG | 0.491256 | 7.3:+6564994 | MS.gene007048:CDS |
| AGTTTGACCTCTAGATGTAA+AGG | 0.494100 | 7.3:-6564802 | None:intergenic |
| GAAGTTCCTGGGCAAAATCC+AGG | 0.501134 | 7.3:+6564864 | MS.gene007048:CDS |
| TTCTTCATGATCCTCATAGT+GGG | 0.502613 | 7.3:+6564682 | MS.gene007048:CDS |
| TGCTGGGACTCAACAAGTGA+AGG | 0.505102 | 7.3:-6564966 | None:intergenic |
| GAACACTCTTCTACTAGTAA+TGG | 0.512065 | 7.3:-6564936 | None:intergenic |
| AGATCCAATACCCCAGCCAT+TGG | 0.519689 | 7.3:-6564643 | None:intergenic |
| GAGTTCACTGCAAGCTCCAA+TGG | 0.520868 | 7.3:+6564627 | MS.gene007048:CDS |
| TTTACAAGAAACCCACTATG+AGG | 0.523336 | 7.3:-6564693 | None:intergenic |
| CAAACTGCTAACATGTCTAA+AGG | 0.546098 | 7.3:+6564359 | None:intergenic |
| ACTACTTCCTTTACATCTAG+AGG | 0.550454 | 7.3:+6564795 | MS.gene007048:CDS |
| CTGCTAACATGTCTAAAGGA+TGG | 0.552141 | 7.3:+6564363 | None:intergenic |
| TTGAAATCCATAAGTTCACC+TGG | 0.559661 | 7.3:-6564882 | None:intergenic |
| TGTTTGTATTGTTGGTGTAG+AGG | 0.561083 | 7.3:+6564719 | MS.gene007048:CDS |
| GTATCATGTTCAATCAGCCC+TGG | 0.564297 | 7.3:+6565154 | MS.gene007048:CDS |
| TTGTTCTGTTGCTGTCGACC+TGG | 0.568093 | 7.3:-6564421 | None:intergenic |
| TAAGTTGTTTGTTCTTAACC+AGG | 0.569810 | 7.3:+6564403 | MS.gene007048:CDS |
| GGTGAACTTATGGATTTCAA+GGG | 0.574063 | 7.3:+6564885 | MS.gene007048:CDS |
| AACAGAACAATATTCTCTGA+TGG | 0.594161 | 7.3:+6564434 | MS.gene007048:CDS |
| CAATGCTAGAAGAAGTTCCT+GGG | 0.601828 | 7.3:+6564853 | MS.gene007048:CDS |
| ACTGCAAGCTCCAATGGCTG+GGG | 0.608500 | 7.3:+6564633 | MS.gene007048:CDS |
| TATCATGTTCAATCAGCCCT+GGG | 0.620754 | 7.3:+6565155 | MS.gene007048:CDS |
| GCTGGGACTCAACAAGTGAA+GGG | 0.631377 | 7.3:-6564965 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTATTTTTCTTATGG+TGG | + | chr7.3:6564462-6564481 | MS.gene007048:intron | 15.0% |
| !!! | TAAGGATCTTCAAATTTTTT+GGG | + | chr7.3:6565091-6565110 | MS.gene007048:CDS | 20.0% |
| ! | AGAAAAGAAGTTGTAAGTTT+AGG | + | chr7.3:6564988-6565007 | MS.gene007048:CDS | 25.0% |
| ! | CAAGACAAATAAAAACCAAA+TGG | - | chr7.3:6565128-6565147 | None:intergenic | 25.0% |
| ! | GAAAAGAAGTTGTAAGTTTA+GGG | + | chr7.3:6564989-6565008 | MS.gene007048:CDS | 25.0% |
| ! | GTAAAAGATGTTTGTATTGT+TGG | + | chr7.3:6564711-6564730 | MS.gene007048:CDS | 25.0% |
| ! | TATGAATGACTTATCTTGTA+AGG | + | chr7.3:6565073-6565092 | MS.gene007048:CDS | 25.0% |
| !!! | AAGTTTTTTGGAGAAAATGA+AGG | + | chr7.3:6565184-6565203 | MS.gene007048:CDS | 25.0% |
| !!! | CATTTGGTTTTTATTTGTCT+TGG | + | chr7.3:6565126-6565145 | MS.gene007048:CDS | 25.0% |
| !!! | GTAAGGATCTTCAAATTTTT+TGG | + | chr7.3:6565090-6565109 | MS.gene007048:CDS | 25.0% |
| AACAGAACAATATTCTCTGA+TGG | + | chr7.3:6564434-6564453 | MS.gene007048:CDS | 30.0% | |
| AAGTTGTAAGTTTAGGGAAT+GGG | + | chr7.3:6564995-6565014 | MS.gene007048:CDS | 30.0% | |
| GAACAACACGTAAAAAGAAA+GGG | - | chr7.3:6564500-6564519 | None:intergenic | 30.0% | |
| TAAGTTGTTTGTTCTTAACC+AGG | + | chr7.3:6564403-6564422 | MS.gene007048:CDS | 30.0% | |
| TGAACAACACGTAAAAAGAA+AGG | - | chr7.3:6564501-6564520 | None:intergenic | 30.0% | |
| ! | CAAAAAACTTTTCATTCCCA+GGG | - | chr7.3:6565174-6565193 | None:intergenic | 30.0% |
| !!! | CTTTCACTTTTCTAGTCTTA+AGG | - | chr7.3:6565052-6565071 | None:intergenic | 30.0% |
| !!! | TTTTTTGGAGAAAATGAAGG+AGG | + | chr7.3:6565187-6565206 | MS.gene007048:CDS | 30.0% |
| ACTACTTCCTTTACATCTAG+AGG | + | chr7.3:6564795-6564814 | MS.gene007048:CDS | 35.0% | |
| AGGTGAACTTATGGATTTCA+AGG | + | chr7.3:6564884-6564903 | MS.gene007048:CDS | 35.0% | |
| AGTTTGACCTCTAGATGTAA+AGG | - | chr7.3:6564805-6564824 | None:intergenic | 35.0% | |
| CTTATGGATTTCAAGGGTTT+AGG | + | chr7.3:6564891-6564910 | MS.gene007048:CDS | 35.0% | |
| GAACACTCTTCTACTAGTAA+TGG | - | chr7.3:6564939-6564958 | None:intergenic | 35.0% | |
| GAAGTTGTAAGTTTAGGGAA+TGG | + | chr7.3:6564994-6565013 | MS.gene007048:CDS | 35.0% | |
| GGTGAACTTATGGATTTCAA+GGG | + | chr7.3:6564885-6564904 | MS.gene007048:CDS | 35.0% | |
| TAGAGGTCAAACTGAAAATC+TGG | + | chr7.3:6564812-6564831 | MS.gene007048:CDS | 35.0% | |
| TGTTTGTATTGTTGGTGTAG+AGG | + | chr7.3:6564719-6564738 | MS.gene007048:CDS | 35.0% | |
| TTCTTCATGATCCTCATAGT+GGG | + | chr7.3:6564682-6564701 | MS.gene007048:CDS | 35.0% | |
| TTGAAATCCATAAGTTCACC+TGG | - | chr7.3:6564885-6564904 | None:intergenic | 35.0% | |
| TTTACAAGAAACCCACTATG+AGG | - | chr7.3:6564696-6564715 | None:intergenic | 35.0% | |
| ! | ACTTACAACTTCTTTTCTGC+TGG | - | chr7.3:6564986-6565005 | None:intergenic | 35.0% |
| ! | CCAAAAAACTTTTCATTCCC+AGG | - | chr7.3:6565175-6565194 | None:intergenic | 35.0% |
| ! | CTTACAACTTCTTTTCTGCT+GGG | - | chr7.3:6564985-6565004 | None:intergenic | 35.0% |
| !!! | CCTGGGAATGAAAAGTTTTT+TGG | + | chr7.3:6565172-6565191 | MS.gene007048:CDS | 35.0% |
| !!! | GGATCTTCAAATTTTTTGGG+AGG | + | chr7.3:6565094-6565113 | MS.gene007048:CDS | 35.0% |
| !!! | TGATTCATGTTTTTTCCCAG+AGG | + | chr7.3:6564596-6564615 | MS.gene007048:intron | 35.0% |
| ATCTGGAAGCTGAAGTCATA+AGG | + | chr7.3:6564829-6564848 | MS.gene007048:CDS | 40.0% | |
| CAATGCTAGAAGAAGTTCCT+GGG | + | chr7.3:6564853-6564872 | MS.gene007048:CDS | 40.0% | |
| GCAAAATCCAGGTGAACTTA+TGG | + | chr7.3:6564875-6564894 | MS.gene007048:CDS | 40.0% | |
| GTTCTTCATGATCCTCATAG+TGG | + | chr7.3:6564681-6564700 | MS.gene007048:CDS | 40.0% | |
| TATCATGTTCAATCAGCCCT+GGG | + | chr7.3:6565155-6565174 | MS.gene007048:CDS | 40.0% | |
| CCAATGCTAGAAGAAGTTCC+TGG | + | chr7.3:6564852-6564871 | MS.gene007048:CDS | 45.0% | |
| CCAGGAACTTCTTCTAGCAT+TGG | - | chr7.3:6564855-6564874 | None:intergenic | 45.0% | |
| GTATCATGTTCAATCAGCCC+TGG | + | chr7.3:6565154-6565173 | MS.gene007048:CDS | 45.0% | |
| !!! | TTTTTTTTTTATTTTTCTTA+TGG | + | chr7.3:6564459-6564478 | MS.gene007048:intron | 5.0% |
| AGATCCAATACCCCAGCCAT+TGG | - | chr7.3:6564646-6564665 | None:intergenic | 50.0% | |
| GAAGTTCCTGGGCAAAATCC+AGG | + | chr7.3:6564864-6564883 | MS.gene007048:CDS | 50.0% | |
| GAATGGGCGTTCACTGCTTT+GGG | + | chr7.3:6565011-6565030 | MS.gene007048:CDS | 50.0% | |
| GAGTTCACTGCAAGCTCCAA+TGG | + | chr7.3:6564627-6564646 | MS.gene007048:CDS | 50.0% | |
| GCTGGGACTCAACAAGTGAA+GGG | - | chr7.3:6564968-6564987 | None:intergenic | 50.0% | |
| TGAACTCGTGTTGAGCCTCT+GGG | - | chr7.3:6564614-6564633 | None:intergenic | 50.0% | |
| TGCTGGGACTCAACAAGTGA+AGG | - | chr7.3:6564969-6564988 | None:intergenic | 50.0% | |
| TGGGAGGAACTTCAGCCATT+TGG | + | chr7.3:6565110-6565129 | MS.gene007048:CDS | 50.0% | |
| TTGTTCTGTTGCTGTCGACC+TGG | - | chr7.3:6564424-6564443 | None:intergenic | 50.0% | |
| ! | AGTTCACCTGGATTTTGCCC+AGG | - | chr7.3:6564873-6564892 | None:intergenic | 50.0% |
| !! | TGGCTGGGGTATTGGATCTT+TGG | + | chr7.3:6564647-6564666 | MS.gene007048:CDS | 50.0% |
| ACTGCAAGCTCCAATGGCTG+GGG | + | chr7.3:6564633-6564652 | MS.gene007048:CDS | 55.0% | |
| AGCTCCAATGGCTGGGGTAT+TGG | + | chr7.3:6564639-6564658 | MS.gene007048:CDS | 55.0% | |
| CACTGCAAGCTCCAATGGCT+GGG | + | chr7.3:6564632-6564651 | MS.gene007048:CDS | 55.0% | |
| GGAATGGGCGTTCACTGCTT+TGG | + | chr7.3:6565010-6565029 | MS.gene007048:CDS | 55.0% | |
| GTGAACTCGTGTTGAGCCTC+TGG | - | chr7.3:6564615-6564634 | None:intergenic | 55.0% | |
| TCACTGCAAGCTCCAATGGC+TGG | + | chr7.3:6564631-6564650 | MS.gene007048:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 6564371 | 6565220 | 6564371 | ID=MS.gene007048 |
| chr7.3 | mRNA | 6564371 | 6565220 | 6564371 | ID=MS.gene007048.t1;Parent=MS.gene007048 |
| chr7.3 | exon | 6564371 | 6564455 | 6564371 | ID=MS.gene007048.t1.exon1;Parent=MS.gene007048.t1 |
| chr7.3 | CDS | 6564371 | 6564455 | 6564371 | ID=cds.MS.gene007048.t1;Parent=MS.gene007048.t1 |
| chr7.3 | exon | 6564616 | 6565220 | 6564616 | ID=MS.gene007048.t1.exon2;Parent=MS.gene007048.t1 |
| chr7.3 | CDS | 6564616 | 6565220 | 6564616 | ID=cds.MS.gene007048.t1;Parent=MS.gene007048.t1 |
| Gene Sequence |
| Protein sequence |
