AlfalfaGEDB | MS.gene038330

M. sativa cultivar XinJiangDaYe / MS.gene038330

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038330.t1	PNX99840.1	73.9	238	55	1	1	231	270	507	1.20E-82	316.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038330.t1	A0A2K3N9Y2	73.9	238	55	1	1	231	270	507	8.9e-83	316.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene038330	MS.gene057069	PPI
MS.gene038330	MS.gene017101	PPI
MS.gene038330	MS.gene017099	PPI
MS.gene038330	MS.gene007048	PPI
MS.gene038330	MS.gene030037	PPI
MS.gene038330	MS.gene007043	PPI
MS.gene038330	MS.gene020137	PPI
MS.gene038330	MS.gene051922	PPI
MS.gene038330	MS.gene057090	PPI
MS.gene038330	MS.gene038300	PPI
MS.gene038323	MS.gene038330	PPI
MS.gene038330	MS.gene46500	PPI
MS.gene038333	MS.gene038330	PPI
MS.gene038330	MS.gene37051	PPI
MS.gene038330	MS.gene057070	PPI
MS.gene23042	MS.gene038330	PPI
MS.gene23045	MS.gene038330	PPI
MS.gene038330	MS.gene030045	PPI
MS.gene038330	MS.gene038342	PPI
MS.gene95230	MS.gene038330	PPI
MS.gene038330	MS.gene071301	PPI
MS.gene038330	MS.gene022811	PPI
MS.gene038330	MS.gene017100	PPI
MS.gene038330	MS.gene063264	PPI
MS.gene038330	MS.gene051917	PPI
MS.gene67155	MS.gene038330	PPI
MS.gene038330	MS.gene051919	PPI
MS.gene007042	MS.gene038330	PPI
MS.gene038330	MS.gene29921	PPI
MS.gene038330	MS.gene051920	PPI
MS.gene038330	MS.gene030026	PPI
MS.gene038330	MS.gene007040	PPI
MS.gene29920	MS.gene038330	PPI
MS.gene038330	MS.gene017102	PPI
MS.gene038330	MS.gene038326	PPI
MS.gene038330	MS.gene017083	PPI
MS.gene007046	MS.gene038330	PPI
MS.gene23044	MS.gene038330	PPI
MS.gene038330	MS.gene053365	PPI
MS.gene038330	MS.gene038324	PPI
MS.gene038330	MS.gene23041	PPI
MS.gene038330	MS.gene051915	PPI
MS.gene038330	MS.gene038328	PPI
MS.gene038330	MS.gene030029	PPI
MS.gene038330	MS.gene022810	PPI
MS.gene038330	MS.gene030042	PPI
MS.gene038330	MS.gene046090	PPI
MS.gene038330	MS.gene057065	PPI
MS.gene038330	MS.gene063219	PPI
MS.gene038330	MS.gene020136	PPI
MS.gene038330	MS.gene23049	PPI
MS.gene038330	MS.gene030034	PPI
MS.gene39218	MS.gene038330	PPI
MS.gene030033	MS.gene038330	PPI
MS.gene038330	MS.gene017115	PPI
MS.gene038330	MS.gene37052	PPI
MS.gene038330	MS.gene65465	PPI
MS.gene038330	MS.gene23047	PPI
MS.gene038330	MS.gene007041	PPI
MS.gene038330	MS.gene057066	PPI
MS.gene038336	MS.gene038330	PPI
MS.gene038330	MS.gene038327	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038330.t1	MTR_5g091180	83.333	204	34	0	1	204	499	702	6.17e-109	328
MS.gene038330.t1	MTR_5g091450	71.967	239	59	2	1	231	554	792	4.36e-107	326
MS.gene038330.t1	MTR_5g091430	68.067	238	69	1	1	231	313	550	6.80e-104	311
MS.gene038330.t1	MTR_5g091410	58.333	192	72	2	47	231	359	549	1.34e-58	194

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 46 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAACCAAGTCTCCTTTAA+AGG	0.155254	5.4:+72203075	None:intergenic
AGCAACTTCTTCAGGAATTA+AGG	0.184830	5.4:-72203105	MS.gene038330:CDS
ATCTTGGATCTCAGCAATTT+TGG	0.221066	5.4:+72202730	None:intergenic
TTAAGGGATATTGCCTTTAA+AGG	0.228541	5.4:-72203088	MS.gene038330:CDS
CAGCACCGACTTGTTCTAAT+TGG	0.250718	5.4:+72202966	None:intergenic
CCTTCAATGCTTCATTATCT+TGG	0.252779	5.4:+72202855	None:intergenic
ATCTTGGTCCGTGCCATTCT+TGG	0.278516	5.4:+72202871	None:intergenic
TCTTATCTTTCTCCTGATCT+TGG	0.325212	5.4:+72202714	None:intergenic
CTCGGCGGCTCAATCTGATT+TGG	0.337029	5.4:-72202606	MS.gene038330:CDS
CCTCCCTGAGCAACTTCTTC+AGG	0.337813	5.4:-72203113	MS.gene038330:CDS
ACTGCGTTGAAGTTAACTTT+AGG	0.349471	5.4:+72203031	None:intergenic
GCAACTTCTTCAGGAATTAA+GGG	0.387841	5.4:-72203104	MS.gene038330:CDS
CAAGAGAGTTGAGTTGTGTT+TGG	0.406897	5.4:+72202786	None:intergenic
CGGACAAGAAGTCTTGAAAC+TGG	0.424617	5.4:-72203143	MS.gene038330:CDS
GAGGTTCTTTCAAATCTTCA+AGG	0.429375	5.4:-72202573	MS.gene038330:CDS
GTTTGTGAAGGAAGCCTTTG+AGG	0.447787	5.4:+72202811	None:intergenic
CCAAGATAATGAAGCATTGA+AGG	0.455948	5.4:-72202855	MS.gene038330:CDS
GAGAGTTGAGTTGTGTTTGG+TGG	0.457037	5.4:+72202789	None:intergenic
AATTCCTGAAGAAGTTGCTC+AGG	0.459517	5.4:+72203109	None:intergenic
AAGAAGTTGCTCAGGGAGGT+TGG	0.466410	5.4:+72203117	None:intergenic
AGAAGTTGCTCAGGGAGGTT+GGG	0.468112	5.4:+72203118	None:intergenic
CTTGATCATCAACGAGGCGT+TGG	0.483231	5.4:+72202657	None:intergenic
CGTTGATGATCAAGTGAAGT+TGG	0.496861	5.4:-72202648	MS.gene038330:CDS
TTGCAAAACTACAAGAAGAC+AGG	0.508255	5.4:-72202754	MS.gene038330:CDS
AATTGCTGAGATCCAAGATC+AGG	0.514938	5.4:-72202726	MS.gene038330:CDS
TATTGCCTTTAAAGGAGACT+TGG	0.518882	5.4:-72203080	MS.gene038330:CDS
GAACAAGACTTAGCCAAGAA+TGG	0.523424	5.4:-72202884	MS.gene038330:CDS
TCCTCACAATGTTGTTGAGA+AGG	0.536532	5.4:+72202936	None:intergenic
AGGTGTTGAAGTTCAAATCT+CGG	0.537620	5.4:-72202624	MS.gene038330:CDS
AAATCTCAGCATTCTGTCCA+CGG	0.543813	5.4:-72203163	MS.gene038330:CDS
TTGTGTTTGGTGGTTTGTGA+AGG	0.561186	5.4:+72202799	None:intergenic
GTTTCAAGACTTCTTGTCCG+TGG	0.567223	5.4:+72203146	None:intergenic
GCCTTCTCAACAACATTGTG+AGG	0.576781	5.4:-72202937	MS.gene038330:CDS
ACAGAAAATATTGACCTCAA+AGG	0.583920	5.4:-72202825	MS.gene038330:CDS
CTGATTTGGAAAGTTATGAG+AGG	0.600308	5.4:-72202592	MS.gene038330:CDS
AGTTAACTTCAACGCAGTCA+AGG	0.610750	5.4:-72203026	MS.gene038330:CDS
ATTCCTGAAGAAGTTGCTCA+GGG	0.621465	5.4:+72203110	None:intergenic
GACTTGTTCTAATTGGAGAG+AGG	0.632105	5.4:+72202973	None:intergenic
TCTCTCCAATTAGAACAAGT+CGG	0.636089	5.4:-72202971	MS.gene038330:CDS
TGCAAAACTACAAGAAGACA+GGG	0.640622	5.4:-72202753	MS.gene038330:CDS
ATTTGATGCTATGGTAGATG+AGG	0.641239	5.4:-72203239	None:intergenic
GATGATCAAGTGAAGTTGGA+AGG	0.655498	5.4:-72202644	MS.gene038330:CDS
AGACTTAGCCAAGAATGGCA+CGG	0.674225	5.4:-72202879	MS.gene038330:CDS
CCTGAAGAAGTTGCTCAGGG+AGG	0.680866	5.4:+72203113	None:intergenic
TGTTGAAGTTCAAATCTCGG+CGG	0.762951	5.4:-72202621	MS.gene038330:CDS
ACTTCACTTGATCATCAACG+AGG	0.807646	5.4:+72202651	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGGTTGAAAAATTTAAAGA+AGG	-	chr5.4:72202724-72202743	MS.gene038330:CDS	20.0%
	ACAGAAAATATTGACCTCAA+AGG	-	chr5.4:72202960-72202979	MS.gene038330:CDS	30.0%
	TTAAGGGATATTGCCTTTAA+AGG	-	chr5.4:72202697-72202716	MS.gene038330:CDS	30.0%
!!	TCGACAAAGTTTTATCAAAG+AGG	-	chr5.4:72202584-72202603	MS.gene038330:CDS	30.0%
	ACTGCGTTGAAGTTAACTTT+AGG	+	chr5.4:72202757-72202776	None:intergenic	35.0%
	AGCAACTTCTTCAGGAATTA+AGG	-	chr5.4:72202680-72202699	MS.gene038330:CDS	35.0%
	CCTTCAATGCTTCATTATCT+TGG	+	chr5.4:72202933-72202952	None:intergenic	35.0%
	GAGGTTCTTTCAAATCTTCA+AGG	-	chr5.4:72203212-72203231	MS.gene038330:CDS	35.0%
	GCAACTTCTTCAGGAATTAA+GGG	-	chr5.4:72202681-72202700	MS.gene038330:CDS	35.0%
	TCTCTCCAATTAGAACAAGT+CGG	-	chr5.4:72202814-72202833	MS.gene038330:CDS	35.0%
	TCTTATCTTTCTCCTGATCT+TGG	+	chr5.4:72203074-72203093	None:intergenic	35.0%
	TGCAAAACTACAAGAAGACA+GGG	-	chr5.4:72203032-72203051	MS.gene038330:CDS	35.0%
	TTCAACCAAGTCTCCTTTAA+AGG	+	chr5.4:72202713-72202732	None:intergenic	35.0%
	TTGCAAAACTACAAGAAGAC+AGG	-	chr5.4:72203031-72203050	MS.gene038330:CDS	35.0%
!	ATCTTGGATCTCAGCAATTT+TGG	+	chr5.4:72203058-72203077	None:intergenic	35.0%
!	TATTGCCTTTAAAGGAGACT+TGG	-	chr5.4:72202705-72202724	MS.gene038330:CDS	35.0%
!!	AGGTGTTGAAGTTCAAATCT+CGG	-	chr5.4:72203161-72203180	MS.gene038330:CDS	35.0%
!!	CCAAGATAATGAAGCATTGA+AGG	-	chr5.4:72202930-72202949	MS.gene038330:CDS	35.0%
!!	CTGATTTGGAAAGTTATGAG+AGG	-	chr5.4:72203193-72203212	MS.gene038330:CDS	35.0%
	AAATCTCAGCATTCTGTCCA+CGG	-	chr5.4:72202622-72202641	MS.gene038330:CDS	40.0%
	AATTCCTGAAGAAGTTGCTC+AGG	+	chr5.4:72202679-72202698	None:intergenic	40.0%
	AATTGCTGAGATCCAAGATC+AGG	-	chr5.4:72203059-72203078	MS.gene038330:CDS	40.0%
	ACTTCACTTGATCATCAACG+AGG	+	chr5.4:72203137-72203156	None:intergenic	40.0%
	AGTTAACTTCAACGCAGTCA+AGG	-	chr5.4:72202759-72202778	MS.gene038330:CDS	40.0%
	ATTCCTGAAGAAGTTGCTCA+GGG	+	chr5.4:72202678-72202697	None:intergenic	40.0%
	GAACAAGACTTAGCCAAGAA+TGG	-	chr5.4:72202901-72202920	MS.gene038330:CDS	40.0%
	GATGATCAAGTGAAGTTGGA+AGG	-	chr5.4:72203141-72203160	MS.gene038330:CDS	40.0%
	TCCTCACAATGTTGTTGAGA+AGG	+	chr5.4:72202852-72202871	None:intergenic	40.0%
	TGTTGAAGTTCAAATCTCGG+CGG	-	chr5.4:72203164-72203183	MS.gene038330:CDS	40.0%
!	CAAGAGAGTTGAGTTGTGTT+TGG	+	chr5.4:72203002-72203021	None:intergenic	40.0%
!	GACTTGTTCTAATTGGAGAG+AGG	+	chr5.4:72202815-72202834	None:intergenic	40.0%
!	TTGTGTTTGGTGGTTTGTGA+AGG	+	chr5.4:72202989-72203008	None:intergenic	40.0%
!!	CGTTGATGATCAAGTGAAGT+TGG	-	chr5.4:72203137-72203156	MS.gene038330:CDS	40.0%
	AGACTTAGCCAAGAATGGCA+CGG	-	chr5.4:72202906-72202925	MS.gene038330:CDS	45.0%
	CGGACAAGAAGTCTTGAAAC+TGG	-	chr5.4:72202642-72202661	MS.gene038330:CDS	45.0%
	GCCTTCTCAACAACATTGTG+AGG	-	chr5.4:72202848-72202867	MS.gene038330:CDS	45.0%
	GTTTCAAGACTTCTTGTCCG+TGG	+	chr5.4:72202642-72202661	None:intergenic	45.0%
	GTTTGTGAAGGAAGCCTTTG+AGG	+	chr5.4:72202977-72202996	None:intergenic	45.0%
!	GAGAGTTGAGTTGTGTTTGG+TGG	+	chr5.4:72202999-72203018	None:intergenic	45.0%
!!	CAGCACCGACTTGTTCTAAT+TGG	+	chr5.4:72202822-72202841	None:intergenic	45.0%
	AAGAAGTTGCTCAGGGAGGT+TGG	+	chr5.4:72202671-72202690	None:intergenic	50.0%
	AGAAGTTGCTCAGGGAGGTT+GGG	+	chr5.4:72202670-72202689	None:intergenic	50.0%
	CTTGATCATCAACGAGGCGT+TGG	+	chr5.4:72203131-72203150	None:intergenic	50.0%
!	ATCTTGGTCCGTGCCATTCT+TGG	+	chr5.4:72202917-72202936	None:intergenic	50.0%
	CCTCCCTGAGCAACTTCTTC+AGG	-	chr5.4:72202672-72202691	MS.gene038330:CDS	55.0%
	CCTGAAGAAGTTGCTCAGGG+AGG	+	chr5.4:72202675-72202694	None:intergenic	55.0%
	CTCGGCGGCTCAATCTGATT+TGG	-	chr5.4:72203179-72203198	MS.gene038330:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	72202556	72203251	72202556	ID=MS.gene038330
chr5.4	mRNA	72202556	72203251	72202556	ID=MS.gene038330.t1;Parent=MS.gene038330
chr5.4	exon	72202556	72203251	72202556	ID=MS.gene038330.t1.exon1;Parent=MS.gene038330.t1
chr5.4	CDS	72202556	72203251	72202556	ID=cds.MS.gene038330.t1;Parent=MS.gene038330.t1

Gene Sequence

>MS.gene038330

ATGGTAGATGAGGATGCTATTGCTGCAATCGACAAAGTTTTATCAAAGAGGATTACTCTTTCTTTAAAATCTCAGCATTCTGTCCACGGACAAGAAGTCTTGAAACTGGATCCCAACCTCCCTGAGCAACTTCTTCAGGAATTAAGGGATATTGCCTTTAAAGGAGACTTGGTTGAAAAATTTAAAGAAGGTCTCACTCCTAAAGTTAACTTCAACGCAGTCAAGGAAAAAATTGACGCTAATGCTCACGCGTTCTCCTCTCTCCAATTAGAACAAGTCGGTGCTGTTGTTAGCCTTCTCAACAACATTGTGAGGATGCTTGAAAAACTTGAAAATTTGAAAAAAGAACAAGACTTAGCCAAGAATGGCACGGACCAAGATAATGAAGCATTGAAGGAGACAAGACAGAAAATATTGACCTCAAAGGCTTCCTTCACAAACCACCAAACACAACTCAACTCTCTTGATGCTCAGATTGCAAAACTACAAGAAGACAGGGCCAAAATTGCTGAGATCCAAGATCAGGAGAAAGATAAGATTATGTCTTTTAACAATGAAGTTAAATCGACTTTCCAACGCCTCGTTGATGATCAAGTGAAGTTGGAAGGTGTTGAAGTTCAAATCTCGGCGGCTCAATCTGATTTGGAAAGTTATGAGAGGTTCTTTCAAATCTTCAAGGCAATCCCTCCATTTTGA

Protein sequence

>MS.gene038330.t1

MVDEDAIAAIDKVLSKRITLSLKSQHSVHGQEVLKLDPNLPEQLLQELRDIAFKGDLVEKFKEGLTPKVNFNAVKEKIDANAHAFSSLQLEQVGAVVSLLNNIVRMLEKLENLKKEQDLAKNGTDQDNEALKETRQKILTSKASFTNHQTQLNSLDAQIAKLQEDRAKIAEIQDQEKDKIMSFNNEVKSTFQRLVDDQVKLEGVEVQISAAQSDLESYERFFQIFKAIPPF