AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030029

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030029.t1 MTR_5g091430 73.438 256 61 1 198 446 295 550 3.52e-122 367
MS.gene030029.t1 MTR_5g091450 73.152 257 61 1 198 446 536 792 1.26e-117 362
MS.gene030029.t1 MTR_5g091450 56.452 186 68 5 1 182 1 177 6.65e-59 206
MS.gene030029.t1 MTR_5g091180 74.236 229 45 2 198 419 481 702 2.82e-98 310
MS.gene030029.t1 MTR_5g091180 51.010 198 83 6 7 193 7 201 1.38e-53 191
MS.gene030029.t1 MTR_5g091410 56.569 274 88 4 193 446 287 549 5.69e-78 252
MS.gene030029.t1 MTR_5g086700 66.225 151 46 2 22 167 41 191 9.54e-62 217
MS.gene030029.t1 MTR_5g086700 66.225 151 46 2 22 167 41 191 1.02e-61 217
MS.gene030029.t1 MTR_3g079840 64.052 153 48 2 22 167 35 187 5.87e-60 212
MS.gene030029.t1 MTR_3g079840 64.052 153 48 2 22 167 34 186 5.97e-60 212
MS.gene030029.t1 MTR_1g041240 55.128 156 66 3 13 167 35 187 4.62e-52 189
MS.gene030029.t1 MTR_1g041240 58.108 148 54 3 27 167 42 188 9.43e-52 188
MS.gene030029.t1 MTR_1g041240 58.108 148 54 3 27 167 40 186 1.09e-51 188
MS.gene030029.t1 MTR_5g091550 46.073 191 82 4 3 192 2 172 2.16e-45 164
MS.gene030029.t1 MTR_5g091550 44.898 196 82 5 3 192 2 177 4.95e-43 158
MS.gene030029.t1 MTR_5g091530 51.562 128 55 3 80 206 4 125 1.75e-29 119
MS.gene030029.t1 MTR_1g042160 37.778 135 81 2 38 169 13 147 7.02e-29 115
MS.gene030029.t1 MTR_7g107200 36.486 148 88 4 38 179 13 160 7.11e-25 105
MS.gene030029.t1 MTR_1g044450 37.692 130 76 3 37 164 64 190 5.28e-20 93.6
MS.gene030029.t1 MTR_1g073790 36.923 130 77 3 37 164 61 187 2.18e-19 91.7
MS.gene030029.t1 MTR_7g107160 33.094 139 80 3 39 165 131 268 2.62e-18 87.4
MS.gene030029.t1 MTR_7g107160 40.909 88 49 2 95 179 4 91 2.47e-13 72.0
MS.gene030029.t1 MTR_1g073800 34.109 129 84 1 37 164 48 176 2.83e-17 84.3
MS.gene030029.t1 MTR_8g011730 35.484 124 74 3 38 156 14 136 1.35e-15 78.2
MS.gene030029.t1 MTR_8g006190 34.167 120 71 3 51 162 5 124 2.13e-15 78.2
MS.gene030029.t1 MTR_3g106700 29.197 137 83 5 36 162 66 198 5.08e-15 78.2
MS.gene030029.t1 MTR_3g106700 29.452 146 87 5 33 164 401 544 2.07e-12 70.1
MS.gene030029.t1 MTR_4g123750 35.043 117 68 4 56 164 40 156 1.03e-13 72.0
MS.gene030029.t1 MTR_4g123750 31.034 145 83 3 33 167 171 308 1.13e-11 65.9
MS.gene030029.t1 MTR_8g006190 33.654 104 61 3 67 162 13 116 6.88e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030029.t1 AT5G06600 58.974 156 63 1 13 167 31 186 7.98e-57 202
MS.gene030029.t1 AT5G06600 58.974 156 63 1 13 167 31 186 9.95e-57 202
MS.gene030029.t1 AT5G06600 58.974 156 63 1 13 167 30 185 1.05e-56 202
MS.gene030029.t1 AT3G11910 56.410 156 67 1 13 167 30 185 1.50e-55 199
MS.gene030029.t1 AT3G11910 56.410 156 67 1 13 167 30 185 1.50e-55 199
MS.gene030029.t1 AT3G11910 56.410 156 67 1 13 167 29 184 1.56e-55 199
MS.gene030029.t1 AT3G11910 56.410 156 67 1 13 167 29 184 1.56e-55 199
MS.gene030029.t1 AT3G58250 41.429 140 79 2 38 174 10 149 4.57e-28 113
MS.gene030029.t1 AT3G58270 42.963 135 76 1 38 171 8 142 6.06e-28 114
MS.gene030029.t1 AT3G58270 42.963 135 76 1 38 171 8 142 6.06e-28 114
MS.gene030029.t1 AT3G58270 42.963 135 76 1 38 171 8 142 6.06e-28 114
MS.gene030029.t1 AT3G58410 38.095 147 86 2 38 181 8 152 5.81e-27 110
MS.gene030029.t1 AT3G58370 40.000 140 80 2 38 173 9 148 1.05e-24 103
MS.gene030029.t1 AT3G58360 36.620 142 83 2 36 173 6 144 4.13e-24 102
MS.gene030029.t1 AT3G58350 34.783 138 86 2 38 171 8 145 8.64e-24 101
MS.gene030029.t1 AT3G58350 34.783 138 86 2 38 171 8 145 8.64e-24 101
MS.gene030029.t1 AT3G58410 35.032 157 83 3 28 181 19 159 4.13e-23 100
MS.gene030029.t1 AT3G58210 36.735 147 87 3 30 173 3 146 2.74e-22 97.8
MS.gene030029.t1 AT3G58340 29.317 249 153 7 38 267 8 252 3.41e-22 97.4
MS.gene030029.t1 AT3G58200 34.969 163 104 2 38 198 8 170 5.00e-22 96.7
MS.gene030029.t1 AT3G58260 37.143 140 83 3 39 173 9 148 1.55e-21 95.5
MS.gene030029.t1 AT3G58340 32.653 147 95 2 38 180 8 154 2.36e-21 94.7
MS.gene030029.t1 AT2G05420 35.762 151 85 4 32 173 5 152 5.12e-21 93.6
MS.gene030029.t1 AT3G27040 34.058 138 89 2 38 173 9 146 1.26e-20 91.7
MS.gene030029.t1 AT2G05410 37.671 146 84 4 31 173 4 145 4.47e-20 90.9
MS.gene030029.t1 AT1G31390 38.211 123 72 2 33 151 3 125 4.83e-19 87.0
MS.gene030029.t1 AT1G04300 38.462 130 75 3 37 164 68 194 8.36e-19 90.1
MS.gene030029.t1 AT1G04300 36.232 138 75 4 37 164 68 202 2.50e-17 85.5
MS.gene030029.t1 AT5G43560 38.095 126 73 3 41 164 73 195 2.52e-17 85.5
MS.gene030029.t1 AT5G43560 38.095 126 73 3 41 164 73 195 2.52e-17 85.5
MS.gene030029.t1 AT5G52330 37.008 127 75 3 41 165 23 146 2.67e-17 84.0
MS.gene030029.t1 AT5G52330 37.008 127 75 3 41 165 23 146 2.67e-17 84.0
MS.gene030029.t1 AT5G52330 37.008 127 75 3 41 165 23 146 2.67e-17 84.0
MS.gene030029.t1 AT3G58290 29.381 194 117 6 38 219 11 196 2.73e-17 82.4
MS.gene030029.t1 AT1G31400 33.793 145 89 4 33 173 3 144 4.65e-17 81.6
MS.gene030029.t1 AT2G01790 32.867 143 92 2 35 173 5 147 9.29e-17 80.5
MS.gene030029.t1 AT3G58440 37.302 126 74 4 38 160 10 133 1.78e-16 82.4
MS.gene030029.t1 AT1G04300 41.441 111 60 3 56 164 68 175 4.33e-16 81.6
MS.gene030029.t1 AT3G58400 31.356 118 80 1 59 175 24 141 1.69e-15 77.4
MS.gene030029.t1 AT3G58220 32.824 131 74 5 39 160 13 138 4.38e-15 76.6
MS.gene030029.t1 AT3G58220 32.824 131 74 5 39 160 13 138 4.46e-15 77.4
MS.gene030029.t1 AT3G58220 32.824 131 74 5 39 160 13 138 1.01e-14 74.3
MS.gene030029.t1 AT3G58220 32.824 131 74 5 39 160 13 138 1.12e-14 75.5
MS.gene030029.t1 AT2G25320 28.467 137 84 5 36 162 71 203 1.15e-14 77.4
MS.gene030029.t1 AT2G25320 28.767 146 87 6 33 164 400 542 5.06e-12 68.9
MS.gene030029.t1 AT2G32870 30.323 155 92 3 25 163 256 410 1.16e-14 75.9
MS.gene030029.t1 AT2G25320 28.467 137 84 5 36 162 70 202 1.19e-14 77.0
MS.gene030029.t1 AT2G25320 28.767 146 87 6 33 164 399 541 5.15e-12 68.9
MS.gene030029.t1 AT3G58220 32.867 143 77 6 39 167 13 150 1.28e-14 75.1
MS.gene030029.t1 AT2G25320 28.467 137 84 5 36 162 71 203 2.30e-14 76.3
MS.gene030029.t1 AT2G25320 28.767 146 87 6 33 164 400 542 1.43e-11 67.4
MS.gene030029.t1 AT3G17380 30.556 180 97 5 56 227 42 201 2.41e-14 73.9
MS.gene030029.t1 AT3G44800 31.159 138 93 2 38 173 5 142 2.65e-14 74.3
MS.gene030029.t1 AT2G42460 35.821 134 81 4 39 170 10 140 8.11e-14 72.4
MS.gene030029.t1 AT2G25330 28.676 136 83 5 37 162 46 177 8.27e-14 73.9
MS.gene030029.t1 AT1G58270 33.600 125 75 3 39 160 254 373 2.75e-13 71.6
MS.gene030029.t1 AT3G44790 32.374 139 88 3 38 174 5 139 4.48e-13 70.5
MS.gene030029.t1 AT3G46190 30.328 122 82 1 39 160 183 301 3.67e-12 67.4
MS.gene030029.t1 AT3G46190 30.328 122 82 1 39 160 163 281 7.69e-12 66.2
MS.gene030029.t1 AT4G09780 31.579 133 88 3 28 157 204 336 3.53e-11 65.1

Find 73 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACCAAGTCCTCTTTAA+AGG 0.173031 5.2:+77265914 None:intergenic
CCTTCAATGCTTCATTATTT+TGG 0.184786 5.2:+77265694 None:intergenic
TCCACAGTGTTGACAGTTTC+AGG 0.199373 5.2:+77267310 None:intergenic
AAGACACGGCTGAGAATTTA+AGG 0.200013 5.2:-77266607 MS.gene030029:CDS
CAACACCGACTTGTTCTAAT+TGG 0.246269 5.2:+77265805 None:intergenic
TTAAAGCTGGGGATTATAAT+TGG 0.278804 5.2:-77267210 MS.gene030029:intron
GAACTGTTTGTCCCTGTATT+TGG 0.285951 5.2:-77267041 MS.gene030029:CDS
CAGTTCACATTGTTCCCTTT+GGG 0.295805 5.2:+77267058 None:intergenic
ACTGCGTTGAAGTTATCTTT+TGG 0.321932 5.2:+77265870 None:intergenic
AGCCTCTGAGCAACTTCTTC+AGG 0.329880 5.2:-77265952 MS.gene030029:CDS
GAGAGTTAAGTTGTGTTTGT+TGG 0.343736 5.2:+77265628 None:intergenic
AGCAAAGACATAAATCAAAA+TGG 0.350329 5.2:+77265360 None:intergenic
CACTTTCTCGTGCGTTGAAC+TGG 0.358575 5.2:+77266750 None:intergenic
CCAAAATAATGAAGCATTGA+AGG 0.364943 5.2:-77265694 MS.gene030029:CDS
ACAGTTCACATTGTTCCCTT+TGG 0.366867 5.2:+77267057 None:intergenic
TCTAATTCAGTTTGAGCCTC+CGG 0.423520 5.2:+77265423 None:intergenic
TATTCAGATGTATTTAAAGC+TGG 0.424191 5.2:-77267223 MS.gene030029:CDS
GTCAAATTGAAAACCGTTGA+AGG 0.435699 5.2:-77265453 MS.gene030029:CDS
TCAACGCACGAGAAAGTGAT+TGG 0.438493 5.2:-77266745 MS.gene030029:CDS
AACCGTTGAAGGTCAAATTC+CGG 0.443893 5.2:-77265442 MS.gene030029:CDS
CATCTGCAACATCCAAATAC+AGG 0.446954 5.2:+77267029 None:intergenic
GATTTGTCTGATCCTGCAAA+AGG 0.447494 5.2:-77266695 MS.gene030029:CDS
ATTCAGATGTATTTAAAGCT+GGG 0.448008 5.2:-77267222 MS.gene030029:CDS
GAGGCTTCTTGTCCTTGTAC+AGG 0.466494 5.2:+77265969 None:intergenic
CTCCGGAATTTGACCTTCAA+CGG 0.466990 5.2:+77265440 None:intergenic
CAACGCACGAGAAAGTGATT+GGG 0.470873 5.2:-77266744 MS.gene030029:CDS
AATAATTACACTGTGAGAAA+AGG 0.470901 5.2:-77266941 MS.gene030029:intron
AAAAGAAAGACAACGAATAC+AGG 0.472256 5.2:-77268069 MS.gene030029:intron
GTCAGGCTGCGCGATTCAAA+TGG 0.472294 5.2:-77267285 MS.gene030029:CDS
GCTGACAAACTTTGAAGTGT+TGG 0.472709 5.2:+77266094 None:intergenic
TGTCAACACTGTGGATAGTC+AGG 0.475646 5.2:-77267302 MS.gene030029:CDS
GAAGCTCTATCGAATCTTCA+AGG 0.476401 5.2:-77265391 MS.gene030029:CDS
GCAACTTCTTCAGGAGTTGA+GGG 0.478662 5.2:-77265943 MS.gene030029:CDS
TATCGCCTTTAAAGAGGACT+TGG 0.484552 5.2:-77265919 MS.gene030029:CDS
GATGCAGTTACTTTGCCATA+TGG 0.487013 5.2:-77267010 MS.gene030029:CDS
CAGTTACTTTGCCATATGGT+TGG 0.487154 5.2:-77267006 MS.gene030029:CDS
AGCAACTTCTTCAGGAGTTG+AGG 0.496043 5.2:-77265944 MS.gene030029:CDS
GCATGTCTGCTCCAACCATA+TGG 0.518309 5.2:+77266995 None:intergenic
GAGGGATATCGCCTTTAAAG+AGG 0.522308 5.2:-77265925 MS.gene030029:CDS
AGACATGCACAGTTTAGCAT+TGG 0.526471 5.2:-77266980 MS.gene030029:CDS
GTCTGTGAAAGAAGTCTTCG+TGG 0.526744 5.2:+77265650 None:intergenic
CAAATCATGAAGAGGCATGA+AGG 0.529644 5.2:+77266712 None:intergenic
CTTGATCATCAACGAGGCGA+TGG 0.532154 5.2:+77265475 None:intergenic
TCCTCACAATGTTGTTGAGA+AGG 0.539367 5.2:+77265775 None:intergenic
AAATCTCAGCATCCTGTACA+AGG 0.540273 5.2:-77265981 MS.gene030029:CDS
GTGTACTTATTTACCCCAAA+GGG 0.553084 5.2:-77267072 MS.gene030029:CDS
AAGACATAAATCAAAATGGA+GGG 0.558434 5.2:+77265364 None:intergenic
AGACACGGCTGAGAATTTAA+GGG 0.559517 5.2:-77266606 MS.gene030029:intron
AGATTGCTGATCTTAAAGCC+AGG 0.563783 5.2:-77265596 MS.gene030029:CDS
ACCTTCTCAACAACATTGTG+AGG 0.565464 5.2:-77265776 MS.gene030029:CDS
ATTTGTCTGATCCTGCAAAA+GGG 0.574728 5.2:-77266694 MS.gene030029:CDS
TTCAGATGTATTTAAAGCTG+GGG 0.580150 5.2:-77267221 MS.gene030029:CDS
GAAGAGATTGAAGAAGGACC+AGG 0.587326 5.2:-77266165 MS.gene030029:CDS
CGTGTACTTATTTACCCCAA+AGG 0.588422 5.2:-77267073 MS.gene030029:CDS
TATGCAGAAGAGATTGAAGA+AGG 0.588849 5.2:-77266171 MS.gene030029:intron
AAGGAGGTGGTGAAAATGAA+AGG 0.597643 5.2:-77268107 MS.gene030029:CDS
AACGCACGAGAAAGTGATTG+GGG 0.605913 5.2:-77266743 MS.gene030029:CDS
AAAGACATAAATCAAAATGG+AGG 0.614222 5.2:+77265363 None:intergenic
AGTTCACATTGTTCCCTTTG+GGG 0.617413 5.2:+77267059 None:intergenic
TCTCTCACAGGCTAGATGGA+AGG 0.618588 5.2:-77268126 None:intergenic
AGACATAAATCAAAATGGAG+GGG 0.626342 5.2:+77265365 None:intergenic
GGATCAGACAAATCATGAAG+AGG 0.629456 5.2:+77266704 None:intergenic
ACAGGCTAGATGGAAGGAGG+TGG 0.630158 5.2:-77268120 None:intergenic
GACTTGTTCTAATTGGTGAG+AGG 0.630409 5.2:+77265812 None:intergenic
CTCACAGGCTAGATGGAAGG+AGG 0.638965 5.2:-77268123 None:intergenic
ATCTGCAACATCCAAATACA+GGG 0.645455 5.2:+77267030 None:intergenic
CAATGTGGATGAGAAAGACA+CGG 0.658001 5.2:-77266621 MS.gene030029:CDS
CTCCTGAAGAAGTTGCTCAG+AGG 0.662017 5.2:+77265950 None:intergenic
CGTTGAAGGTCAAATTCCGG+AGG 0.683920 5.2:-77265439 MS.gene030029:CDS
TCTCACCAATTAGAACAAGT+CGG 0.697541 5.2:-77265810 MS.gene030029:CDS
ACCTGAAACTGTCAACACTG+TGG 0.702025 5.2:-77267311 MS.gene030029:CDS
TGTTAAAGTAACGCGCAATG+TGG 0.724729 5.2:-77266636 MS.gene030029:CDS
ATTTGACTTGATCATCAACG+AGG 0.752249 5.2:+77265469 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAACTGTA+AGG + chr5.2:77266588-77266607 None:intergenic 10.0%
!! GAAATTATCTAAAAATTTAG+TGG + chr5.2:77267107-77267126 None:intergenic 15.0%
!!! TTTGTTATATGATAGAAATT+TGG - chr5.2:77267005-77267024 MS.gene030029:CDS 15.0%
!!! TTTTAGATAATTTCTTAATC+TGG - chr5.2:77267113-77267132 MS.gene030029:intron 15.0%
!! ATCTAAAAATTTAGTGGTTT+GGG + chr5.2:77267101-77267120 None:intergenic 20.0%
!! ATGTTCATTGTATTCATTTA+TGG + chr5.2:77265540-77265559 None:intergenic 20.0%
!! GATTTCAAATACAATTGTAT+AGG - chr5.2:77265918-77265937 MS.gene030029:CDS 20.0%
!! TATCTAAAAATTTAGTGGTT+TGG + chr5.2:77267102-77267121 None:intergenic 20.0%
!! TTAAACTTAAAACTTACATC+AGG + chr5.2:77266960-77266979 None:intergenic 20.0%
!! TTAACAAATTCGAACATTTA+GGG - chr5.2:77267204-77267223 MS.gene030029:intron 20.0%
!! TTCATTTATTAACAACTGTA+AGG + chr5.2:77266628-77266647 None:intergenic 20.0%
!! TTGGTTGAAAAATTTAAACA+AGG - chr5.2:77267585-77267604 MS.gene030029:intron 20.0%
!! TTTAACAAATTCGAACATTT+AGG - chr5.2:77267203-77267222 MS.gene030029:intron 20.0%
!!! AATTTGTTAAAATACACAAC+CGG + chr5.2:77267194-77267213 None:intergenic 20.0%
!!! AGTTTCTCTTATAGTTTTTT+AGG + chr5.2:77265748-77265767 None:intergenic 20.0%
! AATAATTACACTGTGAGAAA+AGG - chr5.2:77266544-77266563 MS.gene030029:intron 25.0%
! AGATATTCCTATGTACTTTA+AGG - chr5.2:77266907-77266926 MS.gene030029:intron 25.0%
! CAAAAATAAAGAGTCATTGA+AGG - chr5.2:77267356-77267375 MS.gene030029:intron 25.0%
! CTCTGTAATATGTAAATCAA+AGG - chr5.2:77265505-77265524 MS.gene030029:CDS 25.0%
! TAACAAATTCGAACATTTAG+GGG - chr5.2:77267205-77267224 MS.gene030029:intron 25.0%
! TATGTTGAAAAGAGAAATGA+TGG - chr5.2:77266660-77266679 MS.gene030029:CDS 25.0%
! TTTATGTCAAAATACACTAC+CGG + chr5.2:77267149-77267168 None:intergenic 25.0%
! TTTGAAAAAAGAGCAAGATT+CGG - chr5.2:77267752-77267771 MS.gene030029:intron 25.0%
!! AAAAGAGAAATGATGGAATT+AGG - chr5.2:77266667-77266686 MS.gene030029:CDS 25.0%
!! ACAATTGTATAGGTTTCTTT+TGG - chr5.2:77265928-77265947 MS.gene030029:CDS 25.0%
!! ATTCAGATGTATTTAAAGCT+GGG - chr5.2:77266263-77266282 MS.gene030029:intron 25.0%
!! CTTTGATTTACATATTACAG+AGG + chr5.2:77265507-77265526 None:intergenic 25.0%
!! TATTCAGATGTATTTAAAGC+TGG - chr5.2:77266262-77266281 MS.gene030029:intron 25.0%
!!! TCAATGACTCTTTATTTTTG+TGG + chr5.2:77267356-77267375 None:intergenic 25.0%
!!! TCTCTGTGGATTTAATTTTT+CGG - chr5.2:77267259-77267278 MS.gene030029:CDS 25.0%
AAAAGAAAGACAACGAATAC+AGG - chr5.2:77265416-77265435 MS.gene030029:CDS 30.0%
AACAAATTCGAACATTTAGG+GGG - chr5.2:77267206-77267225 MS.gene030029:intron 30.0%
AACATAGATGAATTAAGCGT+AGG - chr5.2:77265555-77265574 MS.gene030029:CDS 30.0%
ATATATTCTCCTAAACTGCA+TGG - chr5.2:77266017-77266036 MS.gene030029:CDS 30.0%
ATCACTTACAGTAGAAAAGA+TGG - chr5.2:77265611-77265630 MS.gene030029:CDS 30.0%
ATGACAATCTTAAAGAGAGT+TGG - chr5.2:77265585-77265604 MS.gene030029:CDS 30.0%
CTGTAAGGAACAAAATGAAT+AGG + chr5.2:77266573-77266592 None:intergenic 30.0%
TAACAACTGTAAGGAACAAT+TGG + chr5.2:77266619-77266638 None:intergenic 30.0%
TAACTTCTAAACAAAGTCAG+TGG - chr5.2:77266977-77266996 MS.gene030029:CDS 30.0%
TAGAAAAGATGGTGCAAATT+AGG - chr5.2:77265622-77265641 MS.gene030029:CDS 30.0%
TGACAATCTTAAAGAGAGTT+GGG - chr5.2:77265586-77265605 MS.gene030029:CDS 30.0%
TTAAAGCTGGGGATTATAAT+TGG - chr5.2:77266275-77266294 MS.gene030029:intron 30.0%
TTAATCTGGAAGACTAATTC+CGG - chr5.2:77267127-77267146 MS.gene030029:intron 30.0%
TTTCTATTGTACTATCTCTG+TGG - chr5.2:77267245-77267264 MS.gene030029:CDS 30.0%
! CCTTCAATGCTTCATTATTT+TGG + chr5.2:77267794-77267813 None:intergenic 30.0%
! CTCTTTTCAACATAGATGTA+TGG + chr5.2:77266654-77266673 None:intergenic 30.0%
! GAGGGAAAATAACATAGTTT+TGG + chr5.2:77267062-77267081 None:intergenic 30.0%
! TTCAGATGTATTTAAAGCTG+GGG - chr5.2:77266264-77266283 MS.gene030029:intron 30.0%
! TTCGTTGTCTTTCTTTTCTT+AGG + chr5.2:77265413-77265432 None:intergenic 30.0%
! TTTTAGGCCTTAAAGTACAT+AGG + chr5.2:77266917-77266936 None:intergenic 30.0%
!! AATTGACAGAGTTTTATCAG+AGG - chr5.2:77267464-77267483 MS.gene030029:intron 30.0%
!! ATTGACAGAGTTTTATCAGA+GGG - chr5.2:77267465-77267484 MS.gene030029:intron 30.0%
!! CCAAAATAATGAAGCATTGA+AGG - chr5.2:77267791-77267810 MS.gene030029:intron 30.0%
!! CTCTGTGGATTTAATTTTTC+GGG - chr5.2:77267260-77267279 MS.gene030029:CDS 30.0%
!! TACCTGGCTTGATTTATTTT+AGG + chr5.2:77266933-77266952 None:intergenic 30.0%
!! TTTTGTGGTTTTTTTCTTCC+TGG + chr5.2:77267341-77267360 None:intergenic 30.0%
AATGCTTATCCATGCAGTTT+AGG + chr5.2:77266029-77266048 None:intergenic 35.0%
AATTAGCTGAAAACTTGGAG+AGG + chr5.2:77266101-77266120 None:intergenic 35.0%
ACAAATTCGAACATTTAGGG+GGG - chr5.2:77267207-77267226 MS.gene030029:intron 35.0%
ACATATTACAGAGGCTAAGT+AGG + chr5.2:77265498-77265517 None:intergenic 35.0%
ACTAATATAACAACTCCGAC+AGG + chr5.2:77266332-77266351 None:intergenic 35.0%
ATCCTTACGATAGAATCTAC+CGG + chr5.2:77265678-77265697 None:intergenic 35.0%
ATCTGCAACATCCAAATACA+GGG + chr5.2:77266458-77266477 None:intergenic 35.0%
ATTTGTCTGATCCTGCAAAA+GGG - chr5.2:77266791-77266810 MS.gene030029:intron 35.0%
CAGTGAATTAGCTGAAAACT+TGG + chr5.2:77266106-77266125 None:intergenic 35.0%
CAGTGAGAGATACTTCAATT+AGG - chr5.2:77265979-77265998 MS.gene030029:CDS 35.0%
GACAATCTTAAAGAGAGTTG+GGG - chr5.2:77265587-77265606 MS.gene030029:CDS 35.0%
GAGGATAGTTAGATCACTAA+AGG - chr5.2:77265711-77265730 MS.gene030029:CDS 35.0%
GTCAAATTGAAAACCGTTGA+AGG - chr5.2:77268032-77268051 MS.gene030029:intron 35.0%
GTGTACTTATTTACCCCAAA+GGG - chr5.2:77266413-77266432 MS.gene030029:intron 35.0%
GTTAGATCACTAAAGGTAGA+AGG - chr5.2:77265718-77265737 MS.gene030029:CDS 35.0%
TAACCCCAGTTGTTCATATT+CGG + chr5.2:77267296-77267315 None:intergenic 35.0%
TATGCAGAAGAGATTGAAGA+AGG - chr5.2:77267314-77267333 MS.gene030029:CDS 35.0%
TCTCACCAATTAGAACAAGT+CGG - chr5.2:77267675-77267694 MS.gene030029:intron 35.0%
TGAAAAACTACATGGTGACA+GGG - chr5.2:77267914-77267933 MS.gene030029:intron 35.0%
TGAACCGAATATGAACAACT+GGG - chr5.2:77267289-77267308 MS.gene030029:CDS 35.0%
TTCAACCAAGTCCTCTTTAA+AGG + chr5.2:77267574-77267593 None:intergenic 35.0%
TTGAAAAACTACATGGTGAC+AGG - chr5.2:77267913-77267932 MS.gene030029:intron 35.0%
TTTGGGCAGAGTTCAAATTT+GGG + chr5.2:77267084-77267103 None:intergenic 35.0%
! AAGCCTTATCCAAGTAAGTA+GGG + chr5.2:77265863-77265882 None:intergenic 35.0%
! ACTGCGTTGAAGTTATCTTT+TGG + chr5.2:77267618-77267637 None:intergenic 35.0%
! GAGAGTTAAGTTGTGTTTGT+TGG + chr5.2:77267860-77267879 None:intergenic 35.0%
! GGACAATTGACAGATTTTCT+CGG - chr5.2:77266221-77266240 MS.gene030029:intron 35.0%
! TACAGGATGCTGAGATTTTA+AGG + chr5.2:77267502-77267521 None:intergenic 35.0%
! TTTCTTTTGGTATCCAAGTC+AGG - chr5.2:77265941-77265960 MS.gene030029:CDS 35.0%
!! ACATTCTCTGAAACAGTTCA+TGG - chr5.2:77266299-77266318 MS.gene030029:intron 35.0%
!! ATTTGACTTGATCATCAACG+AGG + chr5.2:77268019-77268038 None:intergenic 35.0%
!! TTGACAGAGTTTTATCAGAG+GGG - chr5.2:77267466-77267485 MS.gene030029:intron 35.0%
AAATCTCAGCATCCTGTACA+AGG - chr5.2:77267504-77267523 MS.gene030029:intron 40.0%
AACCGTTGAAGGTCAAATTC+CGG - chr5.2:77268043-77268062 MS.gene030029:intron 40.0%
AAGACACGGCTGAGAATTTA+AGG - chr5.2:77266878-77266897 MS.gene030029:intron 40.0%
AAGGAGGTGGTGAAAATGAA+AGG - chr5.2:77265378-77265397 MS.gene030029:CDS 40.0%
AAGGATAGTAGTTCAGATGG+AGG - chr5.2:77265692-77265711 MS.gene030029:CDS 40.0%
ACAAGTTCATGAACCTGACT+TGG + chr5.2:77265957-77265976 None:intergenic 40.0%
ACAGTTCACATTGTTCCCTT+TGG + chr5.2:77266431-77266450 None:intergenic 40.0%
ACCTTCTCAACAACATTGTG+AGG - chr5.2:77267709-77267728 MS.gene030029:intron 40.0%
AGACACGGCTGAGAATTTAA+GGG - chr5.2:77266879-77266898 MS.gene030029:intron 40.0%
AGACATGCACAGTTTAGCAT+TGG - chr5.2:77266505-77266524 MS.gene030029:intron 40.0%
AGATGGTGCAAATTAGGCTT+AGG - chr5.2:77265628-77265647 MS.gene030029:CDS 40.0%
AGATTGCTGATCTTAAAGCC+AGG - chr5.2:77267889-77267908 MS.gene030029:intron 40.0%
AGTTCACATTGTTCCCTTTG+GGG + chr5.2:77266429-77266448 None:intergenic 40.0%
CAATGTGGATGAGAAAGACA+CGG - chr5.2:77266864-77266883 MS.gene030029:intron 40.0%
CAGTTCACATTGTTCCCTTT+GGG + chr5.2:77266430-77266449 None:intergenic 40.0%
CATCTGCAACATCCAAATAC+AGG + chr5.2:77266459-77266478 None:intergenic 40.0%
CGTAAGGATAGTAGTTCAGA+TGG - chr5.2:77265689-77265708 MS.gene030029:CDS 40.0%
CGTGTACTTATTTACCCCAA+AGG - chr5.2:77266412-77266431 MS.gene030029:intron 40.0%
CTACTTACTTGGATAAGGCT+TGG - chr5.2:77265862-77265881 MS.gene030029:CDS 40.0%
CTGAACCGAATATGAACAAC+TGG - chr5.2:77267288-77267307 MS.gene030029:CDS 40.0%
GAACCGAATATGAACAACTG+GGG - chr5.2:77267290-77267309 MS.gene030029:CDS 40.0%
GAACTGTTTGTCCCTGTATT+TGG - chr5.2:77266444-77266463 MS.gene030029:intron 40.0%
GAAGCTCTATCGAATCTTCA+AGG - chr5.2:77268094-77268113 MS.gene030029:CDS 40.0%
GATTTGTCTGATCCTGCAAA+AGG - chr5.2:77266790-77266809 MS.gene030029:intron 40.0%
GGATCAGACAAATCATGAAG+AGG + chr5.2:77266784-77266803 None:intergenic 40.0%
GGCCTAAAATAAATCAAGCC+AGG - chr5.2:77266928-77266947 MS.gene030029:intron 40.0%
GTTTGGGCAGAGTTCAAATT+TGG + chr5.2:77267085-77267104 None:intergenic 40.0%
TCCTCACAATGTTGTTGAGA+AGG + chr5.2:77267713-77267732 None:intergenic 40.0%
TCTAATTCAGTTTGAGCCTC+CGG + chr5.2:77268065-77268084 None:intergenic 40.0%
TCTGTATGTAGAGAGAAGAC+CGG - chr5.2:77265656-77265675 MS.gene030029:CDS 40.0%
TGTTAAAGTAACGCGCAATG+TGG - chr5.2:77266849-77266868 MS.gene030029:intron 40.0%
TTTGGTCAAGAACAGTCATC+AGG + chr5.2:77267044-77267063 None:intergenic 40.0%
! CAAATCATGAAGAGGCATGA+AGG + chr5.2:77266776-77266795 None:intergenic 40.0%
! CAAGCCTTATCCAAGTAAGT+AGG + chr5.2:77265864-77265883 None:intergenic 40.0%
! CAGTTACTTTGCCATATGGT+TGG - chr5.2:77266479-77266498 MS.gene030029:intron 40.0%
! GACTTGTTCTAATTGGTGAG+AGG + chr5.2:77267676-77267695 None:intergenic 40.0%
! GATGCAGTTACTTTGCCATA+TGG - chr5.2:77266475-77266494 MS.gene030029:intron 40.0%
! GTGTCACTGTTCTTTGATAC+TGG - chr5.2:77265463-77265482 MS.gene030029:CDS 40.0%
! TATCGCCTTTAAAGAGGACT+TGG - chr5.2:77267566-77267585 MS.gene030029:intron 40.0%
! TTCACAAGATACCCTTTTGC+AGG + chr5.2:77266805-77266824 None:intergenic 40.0%
!! CAACACCGACTTGTTCTAAT+TGG + chr5.2:77267683-77267702 None:intergenic 40.0%
!! GCTGACAAACTTTGAAGTGT+TGG + chr5.2:77267394-77267413 None:intergenic 40.0%
!! TTCTCTGAAACAGTTCATGG+TGG - chr5.2:77266302-77266321 MS.gene030029:intron 40.0%
!!! ACCATGTAGTTTTTCAAGCC+TGG + chr5.2:77267910-77267929 None:intergenic 40.0%
!!! GTTTTTTTTCCACTCTGAGC+AGG - chr5.2:77266389-77266408 MS.gene030029:intron 40.0%
AACGCACGAGAAAGTGATTG+GGG - chr5.2:77266742-77266761 MS.gene030029:CDS 45.0%
ACCTGAAACTGTCAACACTG+TGG - chr5.2:77266174-77266193 MS.gene030029:intron 45.0%
AGCAACTTCTTCAGGAGTTG+AGG - chr5.2:77267541-77267560 MS.gene030029:intron 45.0%
AGTAAGTAGGGTCGCTTACA+TGG + chr5.2:77265851-77265870 None:intergenic 45.0%
CAACGCACGAGAAAGTGATT+GGG - chr5.2:77266741-77266760 MS.gene030029:CDS 45.0%
CACAACCGGAAACGTACTTT+CGG + chr5.2:77267180-77267199 None:intergenic 45.0%
CGACCCTACTTACTTGGATA+AGG - chr5.2:77265857-77265876 MS.gene030029:CDS 45.0%
CTCCGGAATTTGACCTTCAA+CGG + chr5.2:77268048-77268067 None:intergenic 45.0%
GAAGAGATTGAAGAAGGACC+AGG - chr5.2:77267320-77267339 MS.gene030029:CDS 45.0%
GACCGGTAGATTCTATCGTA+AGG - chr5.2:77265673-77265692 MS.gene030029:CDS 45.0%
GCAACTTCTTCAGGAGTTGA+GGG - chr5.2:77267542-77267561 MS.gene030029:intron 45.0%
GCCAGGCTTGAAAAACTACA+TGG - chr5.2:77267906-77267925 MS.gene030029:intron 45.0%
GGCAGAGTTCAAATTTGGGA+GGG + chr5.2:77267080-77267099 None:intergenic 45.0%
GTCTGTGAAAGAAGTCTTCG+TGG + chr5.2:77267838-77267857 None:intergenic 45.0%
TCAACGCACGAGAAAGTGAT+TGG - chr5.2:77266740-77266759 MS.gene030029:CDS 45.0%
TGGTGCAAATTAGGCTTAGG+TGG - chr5.2:77265631-77265650 MS.gene030029:CDS 45.0%
TGTAAGCGACCCTACTTACT+TGG - chr5.2:77265851-77265870 MS.gene030029:CDS 45.0%
TGTCAACACTGTGGATAGTC+AGG - chr5.2:77266183-77266202 MS.gene030029:intron 45.0%
! ACTTACATCAGGTTGCTACC+TGG + chr5.2:77266949-77266968 None:intergenic 45.0%
! CACTACCGAAAGTACGTTTC+CGG - chr5.2:77267172-77267191 MS.gene030029:intron 45.0%
! GAGGGATATCGCCTTTAAAG+AGG - chr5.2:77267560-77267579 MS.gene030029:intron 45.0%
!! TCCACAGTGTTGACAGTTTC+AGG + chr5.2:77266178-77266197 None:intergenic 45.0%
AGCCTCTGAGCAACTTCTTC+AGG - chr5.2:77267533-77267552 MS.gene030029:intron 50.0%
CACTTTCTCGTGCGTTGAAC+TGG + chr5.2:77266738-77266757 None:intergenic 50.0%
CGTTGAAGGTCAAATTCCGG+AGG - chr5.2:77268046-77268065 MS.gene030029:intron 50.0%
CTCCTGAAGAAGTTGCTCAG+AGG + chr5.2:77267538-77267557 None:intergenic 50.0%
CTTGATCATCAACGAGGCGA+TGG + chr5.2:77268013-77268032 None:intergenic 50.0%
GAGGCTTCTTGTCCTTGTAC+AGG + chr5.2:77267519-77267538 None:intergenic 50.0%
GCATGTCTGCTCCAACCATA+TGG + chr5.2:77266493-77266512 None:intergenic 50.0%
GGGCAGAGTTCAAATTTGGG+AGG + chr5.2:77267081-77267100 None:intergenic 50.0%
AAGTACACGCCTGCTCAGAG+TGG + chr5.2:77266401-77266420 None:intergenic 55.0%
GTCAGGCTGCGCGATTCAAA+TGG - chr5.2:77266200-77266219 MS.gene030029:intron 55.0%
GTTCATGGTGGTCAGCCTGT+CGG - chr5.2:77266314-77266333 MS.gene030029:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 77265374 77268133 77265374 ID=MS.gene030029
chr5.2 mRNA 77265374 77268133 77265374 ID=MS.gene030029.t1;Parent=MS.gene030029
chr5.2 exon 77268070 77268133 77268070 ID=MS.gene030029.t1.exon1;Parent=MS.gene030029.t1
chr5.2 CDS 77268070 77268133 77268070 ID=cds.MS.gene030029.t1;Parent=MS.gene030029.t1
chr5.2 exon 77267211 77267343 77267211 ID=MS.gene030029.t1.exon2;Parent=MS.gene030029.t1
chr5.2 CDS 77267211 77267343 77267211 ID=cds.MS.gene030029.t1;Parent=MS.gene030029.t1
chr5.2 exon 77266942 77267096 77266942 ID=MS.gene030029.t1.exon3;Parent=MS.gene030029.t1
chr5.2 CDS 77266942 77267096 77266942 ID=cds.MS.gene030029.t1;Parent=MS.gene030029.t1
chr5.2 exon 77266607 77266782 77266607 ID=MS.gene030029.t1.exon4;Parent=MS.gene030029.t1
chr5.2 CDS 77266607 77266782 77266607 ID=cds.MS.gene030029.t1;Parent=MS.gene030029.t1
chr5.2 exon 77265374 77266186 77265374 ID=MS.gene030029.t1.exon5;Parent=MS.gene030029.t1
chr5.2 CDS 77265374 77266186 77265374 ID=cds.MS.gene030029.t1;Parent=MS.gene030029.t1
Gene Sequence

>MS.gene030029

ATGGAAGGAGGTGGTGAAAATGAAAGGAAAACTTCTCCTAAGAAAAGAAAGACAACGAATACAGGTATTGAATGTCATCATCTTCAATAGTGTCACTGTTCTTTGATACTGGTAATGTCTTCCTACTTAGCCTCTGTAATATGTAAATCAAAGGCTTACATTACCATAAATGAATACAATGAACATAGATGAATTAAGCGTAGGATTATAAATGACAATCTTAAAGAGAGTTGGGGTATCACTTACAGTAGAAAAGATGGTGCAAATTAGGCTTAGGTGGTTTCTGTATGTAGAGAGAAGACCGGTAGATTCTATCGTAAGGATAGTAGTTCAGATGGAGGATAGTTAGATCACTAAAGGTAGAAGGAAGACCTAAAAAACTATAAGAGAAACTATTAAGAAAGATCTAGAGATTAACGAGCTAGATAGAAATATTGTATATGAATATGATAGAACATTTTGACGTCGTTTGATCCATGTAAGCGACCCTACTTACTTGGATAAGGCTTGGTTGTTGTTATTGTTGTCGTAAACATAGATGAATGATTTCAAATACAATTGTATAGGTTTCTTTTGGTATCCAAGTCAGGTTCATGAACTTGTGACAGTGAGAGATACTTCAATTAGGTTCATGAACATCAAAATATATTCTCCTAAACTGCATGGATAAGCATTATCATGTACATTGTCTCGCTGCACTGTAGCACGTCTCTTAATTGTTTTACCTCTCCAAGTTTTCAGCTAATTCACTGCATGATACTTTATTTCTCTTTTCATTTATGAACATTAGCTGCTGCTCTACCTGAAACTGTCAACACTGTGGATAGTCAGGCTGCGCGATTCAAATGGACAATTGACAGATTTTCTCGGCGCATTATGAAAAATCTCTATTCAGATGTATTTAAAGCTGGGGATTATAATTGGTACATTCTCTGAAACAGTTCATGGTGGTCAGCCTGTCGGAGTTGTTATATTAGTTACAATGTGCATTTTATCTTCAGTGATATCGTCTGATGTTTTTTTTCCACTCTGAGCAGGCGTGTACTTATTTACCCCAAAGGGAACAATGTGAACTGTTTGTCCCTGTATTTGGATGTTGCAGATGCAGTTACTTTGCCATATGGTTGGAGCAGACATGCACAGTTTAGCATTGGATTAGTTGATCAAATCAATAATTACACTGTGAGAAAAGGTAGCCTATTCATTTTGTTCCTTACAGTTTTTTTTTTTTTTTTGAACAAGCCAATTGTTCCTTACAGTTGTTAATAAATGAAGTACCATACATCTATGTTGAAAAGAGAAATGATGGAATTAGGTGTGTTATTTTTGTAACTTTATTTGCACTATGTAGAGACACAGCACCAGTTCAACGCACGAGAAAGTGATTGGGGTTTCACATCCTTCATGCCTCTTCATGATTTGTCTGATCCTGCAAAAGGGTATCTTGTGAATGATACTCTTATAGTTGATGTTGATGTTAAAGTAACGCGCAATGTGGATGAGAAAGACACGGCTGAGAATTTAAGGGTAAGTAGATATTCCTATGTACTTTAAGGCCTAAAATAAATCAAGCCAGGTAGCAACCTGATGTAAGTTTTAAGTTTAACTTCTAAACAAAGTCAGTGGACTATTTTGTTATATGATAGAAATTTGGCTATTGCAATCAACCTGATGACTGTTCTTGACCAAAACTATGTTATTTTCCCTCCCAAATTTGAACTCTGCCCAAACCACTAAATTTTTAGATAATTTCTTAATCTGGAAGACTAATTCCGGTAGTGTATTTTGACATAAAATACACTACCGAAAGTACGTTTCCGGTTGTGTATTTTAACAAATTCGAACATTTAGGGGGGCAGATTGAGAAACTATTTCTATTGTACTATCTCTGTGGATTTAATTTTTCGGGAAAATCTGAACCGAATATGAACAACTGGGGTTATGCAGAAGAGATTGAAGAAGGACCAGGAAGAAAAAAACCACAAAAATAAAGAGTCATTGAAGGAGCAAAACAAAGCCAACACTTCAAAGTTTGTCAGCAAAGACTTTGAAGAAATTGATGCTATGATAGATGAAGATGCTGTTGCTGCAATTGACAGAGTTTTATCAGAGGGGATTACTCTTTCCTTAAAATCTCAGCATCCTGTACAAGGACAAGAAGCCTCTGAGCAACTTCTTCAGGAGTTGAGGGATATCGCCTTTAAAGAGGACTTGGTTGAAAAATTTAAACAAGGTCTTTCTCCAAAAGATAACTTCAACGCAGTCAAAGAAAAAATTGATGCTAATGCTGACGTGTTTTCCTCTCACCAATTAGAACAAGTCGGTGTTGTTGTTAACCTTCTCAACAACATTGTGAGGATGTTTGAGAAACTCGAAAATTTGAAAAAAGAGCAAGATTCGGCTAAGAAGAGCACTGACCAAAATAATGAAGCATTGAAGGAAACAAGACAGAAAATATTGACCACGAAGACTTCTTTCACAGACCAACAAACACAACTTAACTCTCTTGACGTTCAGATTGCTGATCTTAAAGCCAGGCTTGAAAAACTACATGGTGACAGGGACAAATTTGTTGAGATTCAAGATCAAGAGAAAGATAAGATCACATCTTTGAACAAAGAAGTCAAGTCAATTTTCCATCGCCTCGTTGATGATCAAGTCAAATTGAAAACCGTTGAAGGTCAAATTCCGGAGGCTCAAACTGAATTAGAAAGTCATGAGAAGCTCTATCGAATCTTCAAGGCAACCCCTCCATTTTGA
Protein sequence

>MS.gene030029.t1

MEGGGENERKTSPKKRKTTNTAAALPETVNTVDSQAARFKWTIDRFSRRIMKNLYSDVFKAGDYNWRVLIYPKGNNVNCLSLYLDVADAVTLPYGWSRHAQFSIGLVDQINNYTVRKETQHQFNARESDWGFTSFMPLHDLSDPAKGYLVNDTLIVDVDVKVTRNVDEKDTAENLRKRLKKDQEEKNHKNKESLKEQNKANTSKFVSKDFEEIDAMIDEDAVAAIDRVLSEGITLSLKSQHPVQGQEASEQLLQELRDIAFKEDLVEKFKQGLSPKDNFNAVKEKIDANADVFSSHQLEQVGVVVNLLNNIVRMFEKLENLKKEQDSAKKSTDQNNEALKETRQKILTTKTSFTDQQTQLNSLDVQIADLKARLEKLHGDRDKFVEIQDQEKDKITSLNKEVKSIFHRLVDDQVKLKTVEGQIPEAQTELESHEKLYRIFKATPPF