AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030026

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030026.t1 MTR_5g091430 90.364 550 51 2 1 548 1 550 0.0 994
MS.gene030026.t1 MTR_5g091450 62.645 605 163 8 4 548 191 792 0.0 683
MS.gene030026.t1 MTR_5g091180 63.238 525 164 8 2 521 202 702 0.0 574
MS.gene030026.t1 MTR_5g091410 58.076 551 197 7 1 548 30 549 0.0 572
MS.gene030026.t1 MTR_5g091530 60.069 288 91 3 4 289 119 384 2.40e-105 321
MS.gene030026.t1 MTR_5g091550 56.169 308 122 4 3 301 180 483 7.95e-103 318
MS.gene030026.t1 MTR_5g091550 56.169 308 122 4 3 301 175 478 8.59e-103 318
MS.gene030026.t1 MTR_5g086700 65.000 260 87 2 4 260 568 826 1.00e-99 326
MS.gene030026.t1 MTR_5g086700 65.000 260 87 2 4 260 568 826 1.06e-99 326
MS.gene030026.t1 MTR_3g079840 61.538 260 96 2 4 260 563 821 1.87e-95 314
MS.gene030026.t1 MTR_3g079840 61.538 260 96 2 4 260 564 822 1.91e-95 314
MS.gene030026.t1 MTR_1g041240 55.000 260 112 3 5 260 565 823 1.01e-79 271
MS.gene030026.t1 MTR_1g041240 55.000 260 112 3 5 260 566 824 1.07e-79 271
MS.gene030026.t1 MTR_1g041240 55.000 260 112 3 5 260 564 822 1.17e-79 271
MS.gene030026.t1 MTR_5g091460 79.545 44 9 0 1 44 1 44 1.04e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030026.t1 AT5G06600 62.844 218 77 2 2 216 562 778 1.63e-79 269
MS.gene030026.t1 AT5G06600 62.844 218 77 2 2 216 561 777 5.02e-79 269
MS.gene030026.t1 AT5G06600 62.844 218 77 2 2 216 562 778 5.27e-79 269
MS.gene030026.t1 AT3G11910 56.540 237 99 2 2 235 560 795 5.91e-78 266
MS.gene030026.t1 AT3G11910 56.540 237 99 2 2 235 560 795 5.91e-78 266
MS.gene030026.t1 AT3G11910 56.540 237 99 2 2 235 559 794 6.04e-78 266
MS.gene030026.t1 AT3G11910 56.540 237 99 2 2 235 559 794 6.04e-78 266

Find 82 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGCAAGGTTAAATAAAT+TGG 0.166835 5.2:-77248066 MS.gene030026:CDS
TTCCAATGATTCTTCATCTT+TGG 0.180925 5.2:+77247289 None:intergenic
TCTTCAATGCTTCATTATTT+TGG 0.190214 5.2:+77246739 None:intergenic
TGACGAATTAAAATTGTTTC+TGG 0.191505 5.2:-77248407 MS.gene030026:CDS
CTTCCAAGAGCAACTTCTTA+TGG 0.228201 5.2:-77246997 MS.gene030026:CDS
TAGCTCAATTCCAGCGCTTT+TGG 0.241563 5.2:-77248934 MS.gene030026:CDS
CCACGAATGCAGTTAGAAAA+TGG 0.246637 5.2:-77247553 MS.gene030026:intron
AATGTTAATTCCTTCTTAAC+TGG 0.258356 5.2:+77247663 None:intergenic
ACAAGGCGACCGGGCTAAAA+TGG 0.260074 5.2:-77246610 MS.gene030026:CDS
GAGGCATTACCCTCATTATT+TGG 0.263040 5.2:+77246430 None:intergenic
TGATACCTTGAAAACGTTAA+AGG 0.272288 5.2:+77249381 None:intergenic
AGTAAGTACATTAATCAAAA+TGG 0.294834 5.2:+77246408 None:intergenic
GAAGATCTTGCAGAACAAAT+TGG 0.323182 5.2:-77249472 MS.gene030026:CDS
CTCTTATTGAATGGCGTAAT+GGG 0.331630 5.2:+77248269 None:intergenic
ATTAAAATTGTTTCTGGAAG+TGG 0.348069 5.2:-77248401 MS.gene030026:CDS
AACATGGTTGCTATAGAGTT+TGG 0.361077 5.2:-77248962 MS.gene030026:CDS
CCAATTCAGTTTGTGCCTCC+AGG 0.376254 5.2:+77246472 None:intergenic
GCCAACACTTTAAAATTTGT+CGG 0.379592 5.2:-77247158 MS.gene030026:CDS
CCTGGAGGCACAAACTGAAT+TGG 0.380178 5.2:-77246472 MS.gene030026:CDS
ATGTGTGTCAAAGGCCGATA+TGG 0.385971 5.2:+77248878 None:intergenic
ACTGGCAAACGCATGACATT+TGG 0.386148 5.2:+77247681 None:intergenic
ATCCAAAGATGAAGAATCAT+TGG 0.396304 5.2:-77247291 MS.gene030026:CDS
CAAGAGAGTTGCGTTGTGTT+TGG 0.402884 5.2:+77246673 None:intergenic
AGAAGTTGCTCTTGGAAGTT+GGG 0.405096 5.2:+77247002 None:intergenic
TCACTTGTACACTATGATAA+AGG 0.412677 5.2:-77249622 MS.gene030026:intron
GGATAAGCCATCAGAAATCT+TGG 0.414878 5.2:-77248084 MS.gene030026:CDS
ATCTTTGGATTCTATATCTG+AGG 0.416621 5.2:+77247304 None:intergenic
AAAAGTTCAATCTCTTCATC+AGG 0.421693 5.2:+77248032 None:intergenic
ACTGTGTTGAAACTAACTTT+AGG 0.426782 5.2:+77246915 None:intergenic
CCTCGTAAAGCTCTTTCAAT+TGG 0.427504 5.2:+77248431 None:intergenic
ATTCCATAAGAAGTTGCTCT+TGG 0.436863 5.2:+77246994 None:intergenic
AATGAAAGCTATTGATTGCC+AGG 0.443087 5.2:-77247449 MS.gene030026:intron
AGAATGAAGGCACATCAAAA+AGG 0.443323 5.2:+77247476 None:intergenic
AAGAAGTTGCTCTTGGAAGT+TGG 0.443347 5.2:+77247001 None:intergenic
TAATCATTGCTCTTATTGAA+TGG 0.444221 5.2:+77248260 None:intergenic
CCCTTTGGTGGCAGTTATGT+TGG 0.447155 5.2:-77248128 MS.gene030026:intron
GCTCTTATTGAATGGCGTAA+TGG 0.454463 5.2:+77248268 None:intergenic
CATGCGTTTGCCAGTTAAGA+AGG 0.456247 5.2:-77247673 MS.gene030026:CDS
AGCAACTTCTTATGGAATTG+AGG 0.457005 5.2:-77246989 MS.gene030026:CDS
GCAACTTCTTATGGAATTGA+GGG 0.458233 5.2:-77246988 MS.gene030026:CDS
GCAAGGTTAAATAAATTGGC+TGG 0.470203 5.2:-77248062 MS.gene030026:CDS
CAACACTGACTTGTTCTAGT+TGG 0.488324 5.2:+77246850 None:intergenic
CATTACCCTCATTATTTGGT+AGG 0.488902 5.2:+77246434 None:intergenic
TTGGTGGCAGTTATGTTGGA+AGG 0.494286 5.2:-77248124 MS.gene030026:intron
TCTATATCTGAGGGCCGAAA+AGG 0.497785 5.2:+77247314 None:intergenic
GCTGGTACCTTTAGCATGAG+TGG 0.499474 5.2:+77247184 None:intergenic
GAGAGTTGCGTTGTGTTTGG+TGG 0.502859 5.2:+77246676 None:intergenic
GCCGATATGGTCGATATGTA+TGG 0.503263 5.2:+77248891 None:intergenic
AAGGCTTATTGTGAACTGTA+AGG 0.503460 5.2:-77248105 MS.gene030026:CDS
AAGCTTATGCTATTGATACT+AGG 0.505022 5.2:-77247510 MS.gene030026:CDS
AACCTTGCCAAGATTTCTGA+TGG 0.506298 5.2:+77248077 None:intergenic
TGAAGCTCATCAGTCAAAAG+TGG 0.513154 5.2:-77247216 MS.gene030026:CDS
ACCATACATATCGACCATAT+CGG 0.517591 5.2:-77248892 MS.gene030026:CDS
GCTTCCTCGATGTGTGTCAA+AGG 0.520515 5.2:+77248869 None:intergenic
TCTGGAAGTGGAGCATGAAC+TGG 0.522119 5.2:-77248389 MS.gene030026:intron
TTTGATCATCAGCGAGGCGA+TGG 0.524961 5.2:+77246523 None:intergenic
AAAATATTATAGCTGAAGAA+AGG 0.525519 5.2:-77247256 MS.gene030026:CDS
AAATAATGAAGCATTGAAGA+AGG 0.539170 5.2:-77246736 MS.gene030026:CDS
AGAAGGCCTACCAAATAATG+AGG 0.539514 5.2:-77246440 MS.gene030026:CDS
AAGTGTTGAAGATAAAATCC+TGG 0.540552 5.2:-77246490 MS.gene030026:CDS
TCTTTGGATTCTATATCTGA+GGG 0.540937 5.2:+77247305 None:intergenic
AGTTAGTTTCAACACAGTCA+TGG 0.546034 5.2:-77246910 MS.gene030026:CDS
ATAGAGACCACTCATGCTAA+AGG 0.550479 5.2:-77247191 MS.gene030026:CDS
ATGATAGACGAGGATGTCAT+TGG 0.551670 5.2:-77247110 MS.gene030026:CDS
ATCGACAAAGTGTTATCAGA+AGG 0.564630 5.2:-77247083 MS.gene030026:CDS
ATCCTGAAAAGAAGAAGCTA+AGG 0.565994 5.2:-77248211 MS.gene030026:intron
ACCTTGTCAACAACATTGCG+AGG 0.587538 5.2:-77246821 MS.gene030026:CDS
ATTAACATTTCAAGAAAGTG+AGG 0.591223 5.2:-77247649 MS.gene030026:intron
ATATCTGAGGGCCGAAAAGG+AGG 0.595378 5.2:+77247317 None:intergenic
TCCTCGCAATGTTGTTGACA+AGG 0.602106 5.2:+77246820 None:intergenic
TGAATTGGAAAGTCATGAGA+AGG 0.602939 5.2:-77246457 MS.gene030026:CDS
TAGTAGACTTTGACAAAGTG+AGG 0.605788 5.2:-77249429 MS.gene030026:CDS
TCGACAAAGTGTTATCAGAA+GGG 0.625778 5.2:-77247082 MS.gene030026:CDS
AAGTACATTAATCAAAATGG+AGG 0.631561 5.2:+77246411 None:intergenic
AGCCATCAGAAATCTTGGCA+AGG 0.646518 5.2:-77248079 MS.gene030026:CDS
GAAGGCCTACCAAATAATGA+GGG 0.655305 5.2:-77246439 MS.gene030026:CDS
CCAATTGAAAGAGCTTTACG+AGG 0.669698 5.2:-77248431 MS.gene030026:CDS
ACTTGATTTGATCATCAGCG+AGG 0.683715 5.2:+77246517 None:intergenic
AATTGATGCTATGATAGACG+AGG 0.695057 5.2:-77247120 MS.gene030026:CDS
GTTGTGTTTGGTGGTCTGTG+AGG 0.712373 5.2:+77246685 None:intergenic
TGTTGAAGATAAAATCCTGG+AGG 0.717731 5.2:-77246487 MS.gene030026:CDS
TCGGCCTTTGACACACATCG+AGG 0.738247 5.2:-77248873 MS.gene030026:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAATCAATATAAAAAA+AGG + chr5.2:77247469-77247488 None:intergenic 10.0%
!! AAACAATCAATATAAAAAAA+GGG + chr5.2:77247468-77247487 None:intergenic 10.0%
!!! TTTTTTATATTGATTGTTTT+TGG - chr5.2:77247469-77247488 MS.gene030026:CDS 10.0%
!!! ATTTCTTGTTTGATTTTAAA+AGG - chr5.2:77247876-77247895 MS.gene030026:intron 15.0%
!! AAAATATTATAGCTGAAGAA+AGG - chr5.2:77248799-77248818 MS.gene030026:intron 20.0%
!! AAAATGAATCTTGTTAATTG+AGG - chr5.2:77246723-77246742 MS.gene030026:CDS 20.0%
!! ATGAAATTAGAGTATGTAAT+TGG + chr5.2:77246829-77246848 None:intergenic 20.0%
!! ATTAAGGGAAAAAAAACTAT+GGG + chr5.2:77247497-77247516 None:intergenic 20.0%
!! CACAAACAAAACAAAATTAT+AGG + chr5.2:77246476-77246495 None:intergenic 20.0%
!! GATAACATATAATACTTCTT+AGG - chr5.2:77246549-77246568 MS.gene030026:CDS 20.0%
!! TAAAATAATTGTTTCCTATG+AGG + chr5.2:77246943-77246962 None:intergenic 20.0%
!! TCAACATTAAACATAAATGA+GGG + chr5.2:77247354-77247373 None:intergenic 20.0%
!! TGAAATTAGAGTATGTAATT+GGG + chr5.2:77246828-77246847 None:intergenic 20.0%
!! TTCAACATTAAACATAAATG+AGG + chr5.2:77247355-77247374 None:intergenic 20.0%
!!! AATTTCATATTTTCTTGCAA+CGG - chr5.2:77246841-77246860 MS.gene030026:CDS 20.0%
!!! TTGGTTGAAAATTTTAAACA+AGG - chr5.2:77249110-77249129 MS.gene030026:intron 20.0%
! AACATATAATACTTCTTAGG+TGG - chr5.2:77246552-77246571 MS.gene030026:CDS 25.0%
! AATACATATGATGTCAACTA+TGG + chr5.2:77247906-77247925 None:intergenic 25.0%
! AATCTTAATAACGAAGCATA+TGG + chr5.2:77248141-77248160 None:intergenic 25.0%
! AATGTTAATTCCTTCTTAAC+TGG + chr5.2:77248395-77248414 None:intergenic 25.0%
! AGAAACAAGATTAGAATAAC+TGG + chr5.2:77248679-77248698 None:intergenic 25.0%
! ATAAAATATGAAGCTTGAAG+GGG + chr5.2:77247271-77247290 None:intergenic 25.0%
! ATATGCTTCGTTATTAAGAT+TGG - chr5.2:77248140-77248159 MS.gene030026:intron 25.0%
! ATTAACATTTCAAGAAAGTG+AGG - chr5.2:77248406-77248425 MS.gene030026:CDS 25.0%
! CAACATTAAACATAAATGAG+GGG + chr5.2:77247353-77247372 None:intergenic 25.0%
! CATAAAATATGAAGCTTGAA+GGG + chr5.2:77247272-77247291 None:intergenic 25.0%
! CATTAAGGGAAAAAAAACTA+TGG + chr5.2:77247498-77247517 None:intergenic 25.0%
! GAAATTAGAGTATGTAATTG+GGG + chr5.2:77246827-77246846 None:intergenic 25.0%
! GACGAACTTCATTATTATAT+AGG - chr5.2:77247755-77247774 MS.gene030026:intron 25.0%
! TAATAATGAAGTTCGTCAAA+AGG + chr5.2:77247752-77247771 None:intergenic 25.0%
! TAATCATTGCTCTTATTGAA+TGG + chr5.2:77247798-77247817 None:intergenic 25.0%
! TATGCTTCGTTATTAAGATT+GGG - chr5.2:77248141-77248160 MS.gene030026:intron 25.0%
! TCATAAAATATGAAGCTTGA+AGG + chr5.2:77247273-77247292 None:intergenic 25.0%
! TGACGAATTAAAATTGTTTC+TGG - chr5.2:77247648-77247667 MS.gene030026:intron 25.0%
! TTAGTAATTACATACCCAAT+TGG - chr5.2:77248432-77248451 MS.gene030026:CDS 25.0%
!! GTCAATTTTTCGTATGTATT+TGG + chr5.2:77246798-77246817 None:intergenic 25.0%
!! GTTATTCTAATCTTGTTTCT+AGG - chr5.2:77248679-77248698 MS.gene030026:intron 25.0%
!! TCTTCAATGCTTCATTATTT+TGG + chr5.2:77249319-77249338 None:intergenic 25.0%
!!! AAATAATGAAGCATTGAAGA+AGG - chr5.2:77249319-77249338 MS.gene030026:intron 25.0%
!!! ATTAAAATTGTTTCTGGAAG+TGG - chr5.2:77247654-77247673 MS.gene030026:CDS 25.0%
!!! GAAGTTGTATACTATTTTGA+TGG - chr5.2:77247704-77247723 MS.gene030026:intron 25.0%
!!! TCTTGTTTGATTTTAAAAGG+TGG - chr5.2:77247879-77247898 MS.gene030026:intron 25.0%
!!! TTTTATGACTTTTTCATCTC+TGG - chr5.2:77247285-77247304 MS.gene030026:CDS 25.0%
AAAAGTTCAATCTCTTCATC+AGG + chr5.2:77248026-77248045 None:intergenic 30.0%
AACCTCAAAAAGAAAATACC+TGG + chr5.2:77248627-77248646 None:intergenic 30.0%
AAGCTTATGCTATTGATACT+AGG - chr5.2:77248545-77248564 MS.gene030026:intron 30.0%
AATTAACCAGCATGTCTTAA+AGG + chr5.2:77246877-77246896 None:intergenic 30.0%
ACATCATATGTATTTCCCTT+TGG - chr5.2:77247912-77247931 MS.gene030026:intron 30.0%
ACTGTGTTGAAACTAACTTT+AGG + chr5.2:77249143-77249162 None:intergenic 30.0%
ACTTCACATCATAGTGAAAT+TGG + chr5.2:77247567-77247586 None:intergenic 30.0%
ATATGACCAACAAGAACAAA+AGG - chr5.2:77248261-77248280 MS.gene030026:CDS 30.0%
ATCATAGTGAAATTGGTATG+TGG + chr5.2:77247560-77247579 None:intergenic 30.0%
ATCCAAAGATGAAGAATCAT+TGG - chr5.2:77248764-77248783 MS.gene030026:intron 30.0%
ATCTTTGGATTCTATATCTG+AGG + chr5.2:77248754-77248773 None:intergenic 30.0%
CTTGGCAAGGTTAAATAAAT+TGG - chr5.2:77247989-77248008 MS.gene030026:intron 30.0%
GTATGTATTTGGCACTAATT+TGG + chr5.2:77246787-77246806 None:intergenic 30.0%
GTGATGTGATATATAGAACA+TGG - chr5.2:77247077-77247096 MS.gene030026:CDS 30.0%
TAAAATATGAAGCTTGAAGG+GGG + chr5.2:77247270-77247289 None:intergenic 30.0%
TATGACCAACAAGAACAAAA+GGG - chr5.2:77248262-77248281 MS.gene030026:CDS 30.0%
TCACTTGTACACTATGATAA+AGG - chr5.2:77246433-77246452 MS.gene030026:CDS 30.0%
TCAGCAGAAAACTAAAAAGT+CGG + chr5.2:77248068-77248087 None:intergenic 30.0%
TCTTTGGATTCTATATCTGA+GGG + chr5.2:77248753-77248772 None:intergenic 30.0%
TGATACCTTGAAAACGTTAA+AGG + chr5.2:77246677-77246696 None:intergenic 30.0%
TGTTTAGTAAATATGCTTGC+TGG - chr5.2:77248185-77248204 MS.gene030026:intron 30.0%
TTCCAATGATTCTTCATCTT+TGG + chr5.2:77248769-77248788 None:intergenic 30.0%
TTTATTTCCTGCACAACAAT+AGG + chr5.2:77248347-77248366 None:intergenic 30.0%
! ATCAAGCTGTTACTTAATTG+CGG - chr5.2:77247393-77247412 MS.gene030026:intron 30.0%
! TCTCTGAAAATTTAACATGC+TGG - chr5.2:77248237-77248256 MS.gene030026:CDS 30.0%
! TCTTGTGGTTTTTATGCTTT+TGG - chr5.2:77247021-77247040 MS.gene030026:CDS 30.0%
! TGAAGAGATTGAACTTTTTG+AGG - chr5.2:77248028-77248047 MS.gene030026:CDS 30.0%
!! AAGTGTTGAAGATAAAATCC+TGG - chr5.2:77249565-77249584 MS.gene030026:intron 30.0%
!! GCCAACACTTTAAAATTTGT+CGG - chr5.2:77248897-77248916 MS.gene030026:CDS 30.0%
!! GCTTTCATTTTCAAGAATGA+AGG + chr5.2:77248595-77248614 None:intergenic 30.0%
!! TATCGCTTTTAAAGAAGACT+TGG - chr5.2:77249091-77249110 MS.gene030026:intron 30.0%
!! TATTTTAGATGCATCCTCAT+AGG - chr5.2:77246926-77246945 MS.gene030026:CDS 30.0%
AAACCAATGAAACAGCAACT+TGG + chr5.2:77248464-77248483 None:intergenic 35.0%
AACATGGTTGCTATAGAGTT+TGG - chr5.2:77247093-77247112 MS.gene030026:CDS 35.0%
AAGGCTTATTGTGAACTGTA+AGG - chr5.2:77247950-77247969 MS.gene030026:intron 35.0%
AATATAAAAAAAGGGCAGCC+CGG + chr5.2:77247460-77247479 None:intergenic 35.0%
AATTCTTCCTATTGTTGTGC+AGG - chr5.2:77248337-77248356 MS.gene030026:intron 35.0%
AATTGATGCTATGATAGACG+AGG - chr5.2:77248935-77248954 MS.gene030026:CDS 35.0%
ACCATACATATCGACCATAT+CGG - chr5.2:77247163-77247182 MS.gene030026:CDS 35.0%
ACCGAAGATTCCATTAGAAA+AGG + chr5.2:77248288-77248307 None:intergenic 35.0%
AGCAACTTCTTATGGAATTG+AGG - chr5.2:77249066-77249085 MS.gene030026:intron 35.0%
ATATGGTTTCTGAACATGCA+AGG + chr5.2:77248124-77248143 None:intergenic 35.0%
ATCCTGAAAAGAAGAAGCTA+AGG - chr5.2:77247844-77247863 MS.gene030026:intron 35.0%
ATTCCATAAGAAGTTGCTCT+TGG + chr5.2:77249064-77249083 None:intergenic 35.0%
CATACGTCATATGAATCTTG+TGG - chr5.2:77247006-77247025 MS.gene030026:CDS 35.0%
CATTACCCTCATTATTTGGT+AGG + chr5.2:77249624-77249643 None:intergenic 35.0%
CTCTTATTGAATGGCGTAAT+GGG + chr5.2:77247789-77247808 None:intergenic 35.0%
GAAGATCTTGCAGAACAAAT+TGG - chr5.2:77246583-77246602 MS.gene030026:CDS 35.0%
GCAACTTCTTATGGAATTGA+GGG - chr5.2:77249067-77249086 MS.gene030026:intron 35.0%
GCAAGGTTAAATAAATTGGC+TGG - chr5.2:77247993-77248012 MS.gene030026:intron 35.0%
TAGTAGACTTTGACAAAGTG+AGG - chr5.2:77246626-77246645 MS.gene030026:CDS 35.0%
TCATATGTATTTCCCTTTGG+TGG - chr5.2:77247915-77247934 MS.gene030026:intron 35.0%
TGAATTGGAAAGTCATGAGA+AGG - chr5.2:77249598-77249617 MS.gene030026:intron 35.0%
TGTTGAAGATAAAATCCTGG+AGG - chr5.2:77249568-77249587 MS.gene030026:intron 35.0%
TTTCTTGCAACGGAATTCAA+TGG - chr5.2:77246851-77246870 MS.gene030026:CDS 35.0%
! AACAAAAGGGCCTTTTCTAA+TGG - chr5.2:77248275-77248294 MS.gene030026:CDS 35.0%
! AATGAAAGCTATTGATTGCC+AGG - chr5.2:77248606-77248625 MS.gene030026:intron 35.0%
! AGAATGAAGGCACATCAAAA+AGG + chr5.2:77248582-77248601 None:intergenic 35.0%
! AGTTAGTTTCAACACAGTCA+TGG - chr5.2:77249145-77249164 MS.gene030026:intron 35.0%
! ATCGACAAAGTGTTATCAGA+AGG - chr5.2:77248972-77248991 MS.gene030026:intron 35.0%
! GACAGAATGCTGAGATTTTA+AGG + chr5.2:77249009-77249028 None:intergenic 35.0%
! GACATCCTTTAACGTTTTCA+AGG - chr5.2:77246669-77246688 MS.gene030026:CDS 35.0%
! GCCTTTTCTAATGGAATCTT+CGG - chr5.2:77248284-77248303 MS.gene030026:intron 35.0%
! TACCTTAGCTTCTTCTTTTC+AGG + chr5.2:77247849-77247868 None:intergenic 35.0%
! TCAAGCTGTTACTTAATTGC+GGG - chr5.2:77247394-77247413 MS.gene030026:intron 35.0%
! TCGACAAAGTGTTATCAGAA+GGG - chr5.2:77248973-77248992 MS.gene030026:intron 35.0%
! TGCCAGGTATTTTCTTTTTG+AGG - chr5.2:77248622-77248641 MS.gene030026:intron 35.0%
! TTTTCCCTTAATGCTTGTGA+GGG - chr5.2:77247505-77247524 MS.gene030026:CDS 35.0%
! TTTTTCCCTTAATGCTTGTG+AGG - chr5.2:77247504-77247523 MS.gene030026:CDS 35.0%
!!! GAGATTGAACTTTTTGAGGT+TGG - chr5.2:77248032-77248051 MS.gene030026:CDS 35.0%
!!! GCCGACAAATTTTAAAGTGT+TGG + chr5.2:77248901-77248920 None:intergenic 35.0%
!!! TTTTAAAGTGTTGGCTTTGC+TGG + chr5.2:77248892-77248911 None:intergenic 35.0%
AACCTTGCCAAGATTTCTGA+TGG + chr5.2:77247981-77248000 None:intergenic 40.0%
AAGAAGTTGCTCTTGGAAGT+TGG + chr5.2:77249057-77249076 None:intergenic 40.0%
AAGATTGGGTCAAGCAATCA+TGG - chr5.2:77248155-77248174 MS.gene030026:intron 40.0%
AGAAGGCCTACCAAATAATG+AGG - chr5.2:77249615-77249634 MS.gene030026:intron 40.0%
AGAAGTTGCTCTTGGAAGTT+GGG + chr5.2:77249056-77249075 None:intergenic 40.0%
ATAAAGGTCCTGCATATGCA+GGG + chr5.2:77247433-77247452 None:intergenic 40.0%
ATAGAGACCACTCATGCTAA+AGG - chr5.2:77248864-77248883 MS.gene030026:CDS 40.0%
ATGATAGACGAGGATGTCAT+TGG - chr5.2:77248945-77248964 MS.gene030026:CDS 40.0%
ATGCAGGACCTTTATGAAAC+CGG - chr5.2:77247438-77247457 MS.gene030026:intron 40.0%
CCAAAAACAAGTCTTGAAGC+TGG - chr5.2:77249028-77249047 MS.gene030026:intron 40.0%
CCAATTGAAAGAGCTTTACG+AGG - chr5.2:77247624-77247643 MS.gene030026:intron 40.0%
CCACGAATGCAGTTAGAAAA+TGG - chr5.2:77248502-77248521 MS.gene030026:intron 40.0%
CCTCGTAAAGCTCTTTCAAT+TGG + chr5.2:77247627-77247646 None:intergenic 40.0%
CTCATTCTGATTCCAATTGC+AGG - chr5.2:77247597-77247616 MS.gene030026:intron 40.0%
CTCTGGTTGTTGACAAATGT+TGG - chr5.2:77247302-77247321 MS.gene030026:CDS 40.0%
CTTCCAAGAGCAACTTCTTA+TGG - chr5.2:77249058-77249077 MS.gene030026:intron 40.0%
GAAGGCCTACCAAATAATGA+GGG - chr5.2:77249616-77249635 MS.gene030026:intron 40.0%
GCCAAGCTTGAAAAACTACA+AGG - chr5.2:77249428-77249447 MS.gene030026:CDS 40.0%
GCTCTTATTGAATGGCGTAA+TGG + chr5.2:77247790-77247809 None:intergenic 40.0%
GGATAAGCCATCAGAAATCT+TGG - chr5.2:77247971-77247990 MS.gene030026:intron 40.0%
TGAAGCTCATCAGTCAAAAG+TGG - chr5.2:77248839-77248858 MS.gene030026:intron 40.0%
TTCTGATTCCAATTGCAGGT+AGG - chr5.2:77247601-77247620 MS.gene030026:intron 40.0%
TTGAAAAACTACAAGGCGAC+CGG - chr5.2:77249435-77249454 MS.gene030026:CDS 40.0%
! AAAGGCCCTTTTGTTCTTGT+TGG + chr5.2:77248270-77248289 None:intergenic 40.0%
! AATTCCAGCGCTTTTGGATA+TGG - chr5.2:77247127-77247146 MS.gene030026:CDS 40.0%
! ACTTGATTTGATCATCAGCG+AGG + chr5.2:77249541-77249560 None:intergenic 40.0%
! ATATGCTTGCTGGTGATGAA+TGG - chr5.2:77248195-77248214 MS.gene030026:intron 40.0%
! ATGAAGGCACATCAAAAAGG+TGG + chr5.2:77248579-77248598 None:intergenic 40.0%
! CCATTTTCTAACTGCATTCG+TGG + chr5.2:77248505-77248524 None:intergenic 40.0%
! GAGGCATTACCCTCATTATT+TGG + chr5.2:77249628-77249647 None:intergenic 40.0%
!! CAACACTGACTTGTTCTAGT+TGG + chr5.2:77249208-77249227 None:intergenic 40.0%
!! CCAGCTTCAAGACTTGTTTT+TGG + chr5.2:77249031-77249050 None:intergenic 40.0%
!! CGAATGTTGTTTCCTTGTTC+AGG - chr5.2:77248713-77248732 MS.gene030026:intron 40.0%
!! TTGTTCAGGTTCCTCCTTTT+CGG - chr5.2:77248727-77248746 MS.gene030026:intron 40.0%
!! TTTTCCATATCCAAAAGCGC+TGG + chr5.2:77247134-77247153 None:intergenic 40.0%
!!! GCCTTGTAGTTTTTCAAGCT+TGG + chr5.2:77249432-77249451 None:intergenic 40.0%
AAGCACCCTCACAAGCATTA+AGG + chr5.2:77247513-77247532 None:intergenic 45.0%
ACCTTGTCAACAACATTGCG+AGG - chr5.2:77249234-77249253 MS.gene030026:intron 45.0%
ACTGGCAAACGCATGACATT+TGG + chr5.2:77248377-77248396 None:intergenic 45.0%
AGCACCCTCACAAGCATTAA+GGG + chr5.2:77247512-77247531 None:intergenic 45.0%
AGCCATCAGAAATCTTGGCA+AGG - chr5.2:77247976-77247995 MS.gene030026:intron 45.0%
ATGTGTGTCAAAGGCCGATA+TGG + chr5.2:77247180-77247199 None:intergenic 45.0%
CAAGAGAGTTGCGTTGTGTT+TGG + chr5.2:77249385-77249404 None:intergenic 45.0%
CATAAAGGTCCTGCATATGC+AGG + chr5.2:77247434-77247453 None:intergenic 45.0%
CATGCGTTTGCCAGTTAAGA+AGG - chr5.2:77248382-77248401 MS.gene030026:intron 45.0%
CCAACATAACTGCCACCAAA+GGG + chr5.2:77247930-77247949 None:intergenic 45.0%
GAAAAGGAGGAACCTGAACA+AGG + chr5.2:77248728-77248747 None:intergenic 45.0%
GCCGATATGGTCGATATGTA+TGG + chr5.2:77247167-77247186 None:intergenic 45.0%
TCCAACATAACTGCCACCAA+AGG + chr5.2:77247931-77247950 None:intergenic 45.0%
TCCTCGCAATGTTGTTGACA+AGG + chr5.2:77249238-77249257 None:intergenic 45.0%
TCTATATCTGAGGGCCGAAA+AGG + chr5.2:77248744-77248763 None:intergenic 45.0%
TGAAAAACTACAAGGCGACC+GGG - chr5.2:77249436-77249455 MS.gene030026:CDS 45.0%
TGCAGGACCTTTATGAAACC+GGG - chr5.2:77247439-77247458 MS.gene030026:intron 45.0%
! TAGCTCAATTCCAGCGCTTT+TGG - chr5.2:77247121-77247140 MS.gene030026:CDS 45.0%
! TCAATTGGCCTACCTGCAAT+TGG + chr5.2:77247612-77247631 None:intergenic 45.0%
! TTGGTGGCAGTTATGTTGGA+AGG - chr5.2:77247931-77247950 MS.gene030026:intron 45.0%
!! ACGCCAAGTTGCTGTTTCAT+TGG - chr5.2:77248458-77248477 MS.gene030026:intron 45.0%
!! CAATGGCCTTTAAGACATGC+TGG - chr5.2:77246868-77246887 MS.gene030026:CDS 45.0%
!! GAATCTCAGCCATTTTAGCC+CGG + chr5.2:77249457-77249476 None:intergenic 45.0%
ATATCTGAGGGCCGAAAAGG+AGG + chr5.2:77248741-77248760 None:intergenic 50.0%
CAACTTGGCGTGCACCAATT+GGG + chr5.2:77248449-77248468 None:intergenic 50.0%
CCAATTCAGTTTGTGCCTCC+AGG + chr5.2:77249586-77249605 None:intergenic 50.0%
GAGAGTTGCGTTGTGTTTGG+TGG + chr5.2:77249382-77249401 None:intergenic 50.0%
GCTGGTACCTTTAGCATGAG+TGG + chr5.2:77248874-77248893 None:intergenic 50.0%
GCTTCCTCGATGTGTGTCAA+AGG + chr5.2:77247189-77247208 None:intergenic 50.0%
TCTGGAAGTGGAGCATGAAC+TGG - chr5.2:77247666-77247685 MS.gene030026:CDS 50.0%
TTTGATCATCAGCGAGGCGA+TGG + chr5.2:77249535-77249554 None:intergenic 50.0%
! CCCTTTGGTGGCAGTTATGT+TGG - chr5.2:77247927-77247946 MS.gene030026:intron 50.0%
! CCTGGAGGCACAAACTGAAT+TGG - chr5.2:77249583-77249602 MS.gene030026:intron 50.0%
! GTTGTGTTTGGTGGTCTGTG+AGG + chr5.2:77249373-77249392 None:intergenic 50.0%
ACAAGGCGACCGGGCTAAAA+TGG - chr5.2:77249445-77249464 MS.gene030026:CDS 55.0%
AGGGCAGCCCGGTTTCATAA+AGG + chr5.2:77247449-77247468 None:intergenic 55.0%
GCAACTTGGCGTGCACCAAT+TGG + chr5.2:77248450-77248469 None:intergenic 55.0%
TCGGCCTTTGACACACATCG+AGG - chr5.2:77247182-77247201 MS.gene030026:CDS 55.0%
TGCATATGCAGGGTCCGAGA+GGG + chr5.2:77247423-77247442 None:intergenic 55.0%
TTAATTGCGGGACCCCCTCT+CGG - chr5.2:77247406-77247425 MS.gene030026:intron 55.0%
CATATGCAGGGTCCGAGAGG+GGG + chr5.2:77247421-77247440 None:intergenic 60.0%
CTCTCGGACCCTGCATATGC+AGG - chr5.2:77247422-77247441 MS.gene030026:intron 60.0%
CTGCATATGCAGGGTCCGAG+AGG + chr5.2:77247424-77247443 None:intergenic 60.0%
GCATATGCAGGGTCCGAGAG+GGG + chr5.2:77247422-77247441 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 77246422 77249655 77246422 ID=MS.gene030026
chr5.2 mRNA 77246422 77249655 77246422 ID=MS.gene030026.t1;Parent=MS.gene030026
chr5.2 exon 77249623 77249655 77249623 ID=MS.gene030026.t1.exon1;Parent=MS.gene030026.t1
chr5.2 CDS 77249623 77249655 77249623 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
chr5.2 exon 77249387 77249506 77249387 ID=MS.gene030026.t1.exon2;Parent=MS.gene030026.t1
chr5.2 CDS 77249387 77249506 77249387 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
chr5.2 exon 77248862 77248984 77248862 ID=MS.gene030026.t1.exon3;Parent=MS.gene030026.t1
chr5.2 CDS 77248862 77248984 77248862 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
chr5.2 exon 77248390 77248458 77248390 ID=MS.gene030026.t1.exon4;Parent=MS.gene030026.t1
chr5.2 CDS 77248390 77248458 77248390 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
chr5.2 exon 77248212 77248300 77248212 ID=MS.gene030026.t1.exon5;Parent=MS.gene030026.t1
chr5.2 CDS 77248212 77248300 77248212 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
chr5.2 exon 77248028 77248136 77248028 ID=MS.gene030026.t1.exon6;Parent=MS.gene030026.t1
chr5.2 CDS 77248028 77248136 77248028 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
chr5.2 exon 77247650 77247718 77247650 ID=MS.gene030026.t1.exon7;Parent=MS.gene030026.t1
chr5.2 CDS 77247650 77247718 77247650 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
chr5.2 exon 77247450 77247563 77247450 ID=MS.gene030026.t1.exon8;Parent=MS.gene030026.t1
chr5.2 CDS 77247450 77247563 77247450 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
chr5.2 exon 77246422 77247342 77246422 ID=MS.gene030026.t1.exon9;Parent=MS.gene030026.t1
chr5.2 CDS 77246422 77247342 77246422 ID=cds.MS.gene030026.t1;Parent=MS.gene030026.t1
Gene Sequence

>MS.gene030026

ATGGCAGAGACTCACTTGTACACTATGATAAAGGTGTTCTAAGCTTGCTTTCCTATAATTTTGTTTTGTTTGTGTCTTTAGAAACATGCTGTTAATTGCTCAATCAGATTGTATTTATATTAATACTGATAACATATAATACTTCTTAGGTGGTACGTGATGAAGATCTTGCAGAACAAATTGGAAATGATATATATTTTGATCTAGTAGACTTTGACAAAGTGAGGAGTTTTCGTGTTCAAAAGCAGACATCCTTTAACGTTTTCAAGGTATCATCATCAGTTACACTTTTATTAGACAAAAAATGAATCTTGTTAATTGAGGATTGAAGTACAAGCATAAATGTACTGAGAATACTGTATCCAAATTAGTGCCAAATACATACGAAAAATTGACAACACTCCCCAATTACATACTCTAATTTCATATTTTCTTGCAACGGAATTCAATGGCCTTTAAGACATGCTGGTTAATTAGTTTGTGAACTGCTTAAGAGAAAATCATTATTTTAGATGCATCCTCATAGGAAACAATTATTTTAAATACAGTTCTGAAATATAAAAATCAAAATGCTTGAAGAAAAACATACGTCATATGAATCTTGTGGTTTTTATGCTTTTGGTTTTGTAATTTATATTTATAACTTATGAATTTCGTGATGTGATATATAGAACATGGTTGCTATAGAGTTTGGCATACTAGCTCAATTCCAGCGCTTTTGGATATGGAAAAAGCGTGTAAACCATACATATCGACCATATCGGCCTTTGACACACATCGAGGAAGCAAAATCTGTAAGTTCATGAGATTGCTTGAAGTTTTTTCAATATTGTTCTGCTCATATACCCCCTTCAAGCTTCATATTTTATGACTTTTTCATCTCTGGTTGTTGACAAATGTTGGTTTTAACTTTTAATAACTTCTTTTACCCCTCATTTATGTTTAATGTTGAATCTGCTCATGACATCTATATCAAGCTGTTACTTAATTGCGGGACCCCCTCTCGGACCCTGCATATGCAGGACCTTTATGAAACCGGGCTGCCCTTTTTTTATATTGATTGTTTTTGGATCCCATAGTTTTTTTTCCCTTAATGCTTGTGAGGGTGCTTAGTTTTTTGTGATTCATGTCTGTACCACATACCAATTTCACTATGATGTGAAGTTAATTTTTTTCTCATTCTGATTCCAATTGCAGGTAGGCCAATTGAAAGAGCTTTACGAGGATGACGAATTAAAATTGTTTCTGGAAGTGGAGCATGAACTGGTAACATTTATATTATGAAGTTGTATACTATTTTGATGGCATGAAACATAATTCAAACAGTCCTTTTGACGAACTTCATTATTATATAGGATTTATATCCCATTACGCCATTCAATAAGAGCAATGATTATATATTACTTTTCTTCAAGTTACACAATCCTGAAAAGAAGAAGCTAAGGTACTGAATCATTTCTTGTTTGATTTTAAAAGGTGGACCATAGTTGACATCATATGTATTTCCCTTTGGTGGCAGTTATGTTGGAAGGCTTATTGTGAACTGTAAGGATAAGCCATCAGAAATCTTGGCAAGGTTAAATAAATTGGCTGGTTATGATCCTGATGAAGAGATTGAACTTTTTGAGGTTGGTTTTTGTTGTCCGACTTTTTAGTTTTCTGCTGAAAAACTTCATAAAAATTTAAATTTGCAACGTATCCTTGCATGTTCAGAAACCATATGCTTCGTTATTAAGATTGGGTCAAGCAATCATGGTTTCAGTTGTTTAGTAAATATGCTTGCTGGTGATGAATGGCGAATAAAGTGCATCGTGTTCTCTGAAAATTTAACATGCTGGCATATGACCAACAAGAACAAAAGGGCCTTTTCTAATGGAATCTTCGGTTGACTTCAATTATTCAGCGTTTGTGAGTAAATTCTTCCTATTGTTGTGCAGGAAATAAAGTTTAAACCAAATGTCATGCGTTTGCCAGTTAAGAAGGAATTAACATTTCAAGAAAGTGAGGTAATTAGTAATTACATACCCAATTGGTGCACGCCAAGTTGCTGTTTCATTGGTTTTCTTATTGATTTGTTTTTCCACGAATGCAGTTAGAAAATGGTGATATTATATGCTTTCAGAAAGCTTATGCTATTGATACTAGGAAATATTTCCACCTTTTTGATGTGCCTTCATTCTTGAAAATGAAAGCTATTGATTGCCAGGTATTTTCTTTTTGAGGTTTAATAAATGCGATACAAAATGAAAGCTAACCAGTTATTCTAATCTTGTTTCTAGGCTTATCTTGAACGAATGTTGTTTCCTTGTTCAGGTTCCTCCTTTTCGGCCCTCAGATATAGAATCCAAAGATGAAGAATCATTGGAAGAGAAAAACAAAAATATTATAGCTGAAGAAAGGAATGTTGTCAAAAATACTGAAGCTCATCAGTCAAAAGTGGATATAGAGACCACTCATGCTAAAGGTACCAGCAAAGCCAACACTTTAAAATTTGTCGGCAAAGACTTGAAAGAAATTGATGCTATGATAGACGAGGATGTCATTGGTGCAATCGACAAAGTGTTATCAGAAGGGATTACTCTTTCCTTAAAATCTCAGCATTCTGTCCAAAAACAAGTCTTGAAGCTGGATCCCAACTTCCAAGAGCAACTTCTTATGGAATTGAGGGATATCGCTTTTAAAGAAGACTTGGTTGAAAATTTTAAACAAGGTCTCACTCCTAAAGTTAGTTTCAACACAGTCATGGAAAAAATTGAAGCTAATATTGACATGTTCTCTTCTCACCAACTAGAACAAGTCAGTGTTGTTGTTAACCTTGTCAACAACATTGCGAGGATGTTTGAGCAACTTAAAAATTTGAAAAAATTGCAAGACTCGACTAAGAAGAGCACTGACCAAAATAATGAAGCATTGAAGAAGGCAAAAAAAATTTTGATCTCAAAGACGTGCCTCACAGACCACCAAACACAACGCAACTCTCTTGACATTCAGATTGCTGATATCAAAGCCAAGCTTGAAAAACTACAAGGCGACCGGGCTAAAATGGCTGAGATTCAAGATCAAGAGAAACATAAGATAACATCTTTTAACAATGAAGTTAAATCAATTTTCCATCGCCTCGCTGATGATCAAATCAAGTTGAAAAGTGTTGAAGATAAAATCCTGGAGGCACAAACTGAATTGGAAAGTCATGAGAAGGCCTACCAAATAATGAGGGTAATGCCTCCATTTTGA
Protein sequence

>MS.gene030026.t1

MAETHLYTMIKVVRDEDLAEQIGNDIYFDLVDFDKVRSFRVQKQTSFNVFKNMVAIEFGILAQFQRFWIWKKRVNHTYRPYRPLTHIEEAKSVGQLKELYEDDELKLFLEVEHELDLYPITPFNKSNDYILLFFKLHNPEKKKLSYVGRLIVNCKDKPSEILARLNKLAGYDPDEEIELFEEIKFKPNVMRLPVKKELTFQESELENGDIICFQKAYAIDTRKYFHLFDVPSFLKMKAIDCQVPPFRPSDIESKDEESLEEKNKNIIAEERNVVKNTEAHQSKVDIETTHAKGTSKANTLKFVGKDLKEIDAMIDEDVIGAIDKVLSEGITLSLKSQHSVQKQVLKLDPNFQEQLLMELRDIAFKEDLVENFKQGLTPKVSFNTVMEKIEANIDMFSSHQLEQVSVVVNLVNNIARMFEQLKNLKKLQDSTKKSTDQNNEALKKAKKILISKTCLTDHQTQRNSLDIQIADIKAKLEKLQGDRAKMAEIQDQEKHKITSFNNEVKSIFHRLADDQIKLKSVEDKILEAQTELESHEKAYQIMRVMPPF