AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene038327

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038327.t1 MTR_5g091430 73.438 256 61 1 198 446 295 550 2.44e-121 364
MS.gene038327.t1 MTR_5g091450 73.152 257 61 1 198 446 536 792 4.11e-117 361
MS.gene038327.t1 MTR_5g091450 55.914 186 69 5 1 182 1 177 7.06e-59 206
MS.gene038327.t1 MTR_5g091180 74.236 229 45 2 198 419 481 702 8.32e-98 308
MS.gene038327.t1 MTR_5g091180 50.505 198 84 6 7 193 7 201 1.39e-53 191
MS.gene038327.t1 MTR_5g091410 64.444 225 76 2 222 446 329 549 7.03e-79 254
MS.gene038327.t1 MTR_5g086700 65.563 151 47 2 22 167 41 191 1.09e-61 217
MS.gene038327.t1 MTR_5g086700 65.563 151 47 2 22 167 41 191 1.11e-61 217
MS.gene038327.t1 MTR_3g079840 63.399 153 49 2 22 167 35 187 7.25e-60 211
MS.gene038327.t1 MTR_3g079840 63.399 153 49 2 22 167 34 186 7.45e-60 211
MS.gene038327.t1 MTR_1g041240 54.487 156 67 3 13 167 35 187 4.94e-52 189
MS.gene038327.t1 MTR_1g041240 57.432 148 55 3 27 167 40 186 1.10e-51 187
MS.gene038327.t1 MTR_1g041240 57.432 148 55 3 27 167 42 188 1.12e-51 187
MS.gene038327.t1 MTR_5g091550 45.550 191 83 4 3 192 2 172 3.59e-45 164
MS.gene038327.t1 MTR_5g091550 44.388 196 83 5 3 192 2 177 7.58e-43 157
MS.gene038327.t1 MTR_5g091530 50.781 128 56 3 80 206 4 125 2.48e-29 118
MS.gene038327.t1 MTR_1g042160 37.778 135 81 2 38 169 13 147 4.80e-29 116
MS.gene038327.t1 MTR_7g107200 36.486 148 88 4 38 179 13 160 5.17e-25 106
MS.gene038327.t1 MTR_1g044450 36.923 130 77 3 37 164 64 190 7.50e-20 93.2
MS.gene038327.t1 MTR_1g073790 36.154 130 78 3 37 164 61 187 2.85e-19 91.3
MS.gene038327.t1 MTR_7g107160 33.094 139 80 3 39 165 131 268 1.79e-18 87.8
MS.gene038327.t1 MTR_7g107160 40.909 88 49 2 95 179 4 91 1.73e-13 72.4
MS.gene038327.t1 MTR_1g073800 34.884 129 83 1 37 164 48 176 1.90e-17 84.7
MS.gene038327.t1 MTR_8g006190 34.167 120 71 3 51 162 5 124 1.65e-15 78.6
MS.gene038327.t1 MTR_8g011730 35.484 124 74 3 38 156 14 136 1.77e-15 77.8
MS.gene038327.t1 MTR_3g106700 28.467 137 84 5 36 162 66 198 6.06e-15 78.2
MS.gene038327.t1 MTR_3g106700 28.767 146 88 5 33 164 401 544 2.76e-12 69.7
MS.gene038327.t1 MTR_4g123750 35.043 117 68 4 56 164 40 156 1.06e-13 72.0
MS.gene038327.t1 MTR_4g123750 31.034 145 83 3 33 167 171 308 1.00e-11 66.2
MS.gene038327.t1 MTR_8g006190 33.654 104 61 3 67 162 13 116 5.51e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038327.t1 AT5G06600 58.333 156 64 1 13 167 31 186 8.06e-57 202
MS.gene038327.t1 AT5G06600 58.333 156 64 1 13 167 31 186 1.11e-56 202
MS.gene038327.t1 AT5G06600 58.333 156 64 1 13 167 30 185 1.23e-56 202
MS.gene038327.t1 AT3G11910 55.769 156 68 1 13 167 29 184 1.56e-55 199
MS.gene038327.t1 AT3G11910 55.769 156 68 1 13 167 29 184 1.56e-55 199
MS.gene038327.t1 AT3G11910 55.769 156 68 1 13 167 30 185 1.61e-55 199
MS.gene038327.t1 AT3G11910 55.769 156 68 1 13 167 30 185 1.61e-55 199
MS.gene038327.t1 AT3G58250 40.714 140 80 2 38 174 10 149 6.61e-28 113
MS.gene038327.t1 AT3G58270 42.222 135 77 1 38 171 8 142 8.66e-28 113
MS.gene038327.t1 AT3G58270 42.222 135 77 1 38 171 8 142 8.66e-28 113
MS.gene038327.t1 AT3G58270 42.222 135 77 1 38 171 8 142 8.66e-28 113
MS.gene038327.t1 AT3G58410 37.415 147 87 2 38 181 8 152 8.47e-27 110
MS.gene038327.t1 AT3G58370 40.714 140 79 2 38 173 9 148 6.87e-25 104
MS.gene038327.t1 AT3G58360 36.620 142 83 2 36 173 6 144 3.08e-24 102
MS.gene038327.t1 AT3G58350 34.058 138 87 2 38 171 8 145 1.38e-23 100
MS.gene038327.t1 AT3G58350 34.058 138 87 2 38 171 8 145 1.38e-23 100
MS.gene038327.t1 AT3G58410 35.032 157 83 3 28 181 19 159 3.50e-23 100
MS.gene038327.t1 AT3G58340 28.968 252 150 7 38 267 8 252 2.39e-22 97.8
MS.gene038327.t1 AT3G58210 36.054 147 88 3 30 173 3 146 4.03e-22 97.1
MS.gene038327.t1 AT3G58200 34.783 161 103 2 38 196 8 168 7.43e-22 96.3
MS.gene038327.t1 AT3G58260 36.429 140 84 3 39 173 9 148 2.02e-21 95.1
MS.gene038327.t1 AT3G58340 28.400 250 144 7 38 267 8 242 2.78e-21 94.4
MS.gene038327.t1 AT2G05420 35.099 151 86 4 32 173 5 152 7.38e-21 92.8
MS.gene038327.t1 AT3G27040 33.333 138 90 2 38 173 9 146 2.07e-20 91.3
MS.gene038327.t1 AT2G05410 36.986 146 85 4 31 173 4 145 7.15e-20 90.1
MS.gene038327.t1 AT1G04300 38.462 130 75 3 37 164 68 194 6.39e-19 90.5
MS.gene038327.t1 AT1G31390 37.398 123 73 2 33 151 3 125 6.89e-19 86.7
MS.gene038327.t1 AT1G04300 36.232 138 75 4 37 164 68 202 1.88e-17 85.9
MS.gene038327.t1 AT5G52330 37.008 127 75 3 41 165 23 146 2.05e-17 84.3
MS.gene038327.t1 AT5G52330 37.008 127 75 3 41 165 23 146 2.05e-17 84.3
MS.gene038327.t1 AT5G52330 37.008 127 75 3 41 165 23 146 2.05e-17 84.3
MS.gene038327.t1 AT5G43560 38.095 126 73 3 41 164 73 195 2.96e-17 85.1
MS.gene038327.t1 AT5G43560 38.095 126 73 3 41 164 73 195 2.96e-17 85.1
MS.gene038327.t1 AT1G31400 33.103 145 90 4 33 173 3 144 5.41e-17 81.3
MS.gene038327.t1 AT3G58290 29.016 193 117 6 38 218 11 195 6.41e-17 81.3
MS.gene038327.t1 AT3G58440 37.302 126 74 4 38 160 10 133 1.30e-16 82.8
MS.gene038327.t1 AT2G01790 32.168 143 93 2 35 173 5 147 1.44e-16 80.1
MS.gene038327.t1 AT1G04300 41.441 111 60 3 56 164 68 175 3.21e-16 82.0
MS.gene038327.t1 AT3G58400 30.508 118 81 1 59 175 24 141 2.39e-15 76.6
MS.gene038327.t1 AT3G58220 32.061 131 75 5 39 160 13 138 6.38e-15 76.3
MS.gene038327.t1 AT3G58220 32.061 131 75 5 39 160 13 138 6.39e-15 77.0
MS.gene038327.t1 AT2G32870 31.613 155 88 5 25 162 256 409 8.86e-15 76.3
MS.gene038327.t1 AT3G58220 32.061 131 75 5 39 160 13 138 1.16e-14 74.3
MS.gene038327.t1 AT3G58220 32.061 131 75 5 39 160 13 138 1.24e-14 75.5
MS.gene038327.t1 AT3G58220 32.168 143 78 6 39 167 13 150 1.40e-14 74.7
MS.gene038327.t1 AT2G25320 27.737 137 85 5 36 162 70 202 1.76e-14 76.6
MS.gene038327.t1 AT2G25320 28.082 146 88 6 33 164 399 541 7.90e-12 68.2
MS.gene038327.t1 AT2G25320 27.737 137 85 5 36 162 71 203 1.80e-14 76.6
MS.gene038327.t1 AT2G25320 28.082 146 88 6 33 164 400 542 8.11e-12 68.2
MS.gene038327.t1 AT3G17380 35.088 114 69 2 56 164 42 155 2.57e-14 73.9
MS.gene038327.t1 AT2G25320 27.737 137 85 5 36 162 71 203 3.46e-14 75.9
MS.gene038327.t1 AT2G25320 28.082 146 88 6 33 164 400 542 2.29e-11 66.6
MS.gene038327.t1 AT3G44800 30.435 138 94 2 38 173 5 142 4.95e-14 73.6
MS.gene038327.t1 AT2G25330 29.412 136 82 5 37 162 46 177 5.22e-14 74.7
MS.gene038327.t1 AT2G42460 35.821 134 81 4 39 170 10 140 5.65e-14 72.8
MS.gene038327.t1 AT1G58270 34.400 125 74 3 39 160 254 373 1.74e-13 72.4
MS.gene038327.t1 AT3G44790 31.655 139 89 3 38 174 5 139 6.96e-13 69.7
MS.gene038327.t1 AT3G46190 30.328 122 82 1 39 160 183 301 3.00e-12 67.8
MS.gene038327.t1 AT3G46190 30.328 122 82 1 39 160 163 281 6.46e-12 66.6
MS.gene038327.t1 AT4G09780 31.579 133 88 3 28 157 204 336 3.20e-11 65.5
MS.gene038327.t1 AT3G58430 28.696 230 132 10 38 248 8 224 8.76e-11 64.3

Find 71 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACCAAGTCCTCTTTAA+AGG 0.173031 5.4:+72185333 None:intergenic
CCTTCAATGCTTCATTATTT+TGG 0.184786 5.4:+72185113 None:intergenic
TCCACAGTGTTGACAGTTTC+AGG 0.199373 5.4:+72186669 None:intergenic
AAGACACGGCTGAGAATTTA+AGG 0.200013 5.4:-72185964 MS.gene038327:CDS
CAACACCGACTTGTTCTAAT+TGG 0.246269 5.4:+72185224 None:intergenic
TTAAAGCTGGGGATTATAAT+TGG 0.278804 5.4:-72186569 MS.gene038327:intron
GAACTGTTTGTCCCTGTATT+TGG 0.285951 5.4:-72186400 MS.gene038327:CDS
CAGTTCACATTGTTCCCTTT+GGG 0.295805 5.4:+72186417 None:intergenic
ACTGCATTGAAGTTATCTTT+TGG 0.298043 5.4:+72185289 None:intergenic
AGCCTCTGAGCAACTTCTTC+AGG 0.329880 5.4:-72185371 MS.gene038327:CDS
AGCAAAGACATAAATCAAAA+TGG 0.350329 5.4:+72184779 None:intergenic
CACTTTCTCGTGCGTTGAAC+TGG 0.358575 5.4:+72186107 None:intergenic
CCAAAATAATGAAGCATTGA+AGG 0.364943 5.4:-72185113 MS.gene038327:CDS
ACAGTTCACATTGTTCCCTT+TGG 0.366867 5.4:+72186416 None:intergenic
TCTAATTCAGTTTGAGCCTC+CGG 0.423520 5.4:+72184842 None:intergenic
TATTCAGATGTATTTAAAGC+TGG 0.424191 5.4:-72186582 MS.gene038327:CDS
GTCAAATTGAAAACCGTTGA+AGG 0.435699 5.4:-72184872 MS.gene038327:CDS
TCAACGCACGAGAAAGTGAT+TGG 0.438493 5.4:-72186102 MS.gene038327:CDS
AACCGTTGAAGGTCAAATTC+CGG 0.443893 5.4:-72184861 MS.gene038327:CDS
CATCTGCAACATCCAAATAC+AGG 0.446954 5.4:+72186388 None:intergenic
GATTTGTCTGATCCTGCAAA+AGG 0.447494 5.4:-72186052 MS.gene038327:CDS
ATTCAGATGTATTTAAAGCT+GGG 0.448008 5.4:-72186581 MS.gene038327:CDS
GTCTGTGAAAGAAGTCTTTG+TGG 0.466674 5.4:+72185069 None:intergenic
CTCCGGAATTTGACCTTCAA+CGG 0.466990 5.4:+72184859 None:intergenic
CAACGCACGAGAAAGTGATT+GGG 0.470873 5.4:-72186101 MS.gene038327:CDS
AATAATTACACTGTGAGAAA+AGG 0.470901 5.4:-72186300 MS.gene038327:intron
AAAAGAAAGACAACGAATAC+AGG 0.472256 5.4:-72187433 MS.gene038327:intron
GTCAGGCTGCGCGATTCAAA+TGG 0.472294 5.4:-72186644 MS.gene038327:CDS
GCTGACAAACTTTGAAGTGT+TGG 0.472709 5.4:+72185513 None:intergenic
TGTCAACACTGTGGATAGTC+AGG 0.475646 5.4:-72186661 MS.gene038327:CDS
GAAGCTCTATCGAATCTTCA+AGG 0.476401 5.4:-72184810 MS.gene038327:CDS
GCAACTTCTTCAGGAGTTGA+GGG 0.478662 5.4:-72185362 MS.gene038327:CDS
TATCGCCTTTAAAGAGGACT+TGG 0.484552 5.4:-72185338 MS.gene038327:CDS
GATGCAGTTACTTTGCCATA+TGG 0.487013 5.4:-72186369 MS.gene038327:CDS
CAGTTACTTTGCCATATGGT+TGG 0.487154 5.4:-72186365 MS.gene038327:CDS
AGCAACTTCTTCAGGAGTTG+AGG 0.496043 5.4:-72185363 MS.gene038327:CDS
AAATCTCAGCATTCTGTACA+AGG 0.508974 5.4:-72185400 MS.gene038327:CDS
GCATGTCTGCTCCAACCATA+TGG 0.518309 5.4:+72186354 None:intergenic
GAGGGATATCGCCTTTAAAG+AGG 0.522308 5.4:-72185344 MS.gene038327:CDS
AGACATGCACAGTTTAGCAT+TGG 0.526471 5.4:-72186339 MS.gene038327:CDS
CAAATCATGAAGAGGCATGA+AGG 0.529644 5.4:+72186069 None:intergenic
CTTGATCATCAACGAGGCGA+TGG 0.532154 5.4:+72184894 None:intergenic
TCCTCACAATGTTGTTGAGA+AGG 0.539367 5.4:+72185194 None:intergenic
AGATTGCTGACCTTAAAGCC+AGG 0.552116 5.4:-72185015 MS.gene038327:CDS
GTGTACTTATTTACCCCAAA+GGG 0.553084 5.4:-72186431 MS.gene038327:CDS
AAGACATAAATCAAAATGGA+GGG 0.558434 5.4:+72184783 None:intergenic
AGACACGGCTGAGAATTTAA+GGG 0.559517 5.4:-72185963 MS.gene038327:intron
ACCTTCTCAACAACATTGTG+AGG 0.565464 5.4:-72185195 MS.gene038327:CDS
ATTTGTCTGATCCTGCAAAA+GGG 0.574728 5.4:-72186051 MS.gene038327:CDS
TTCAGATGTATTTAAAGCTG+GGG 0.580150 5.4:-72186580 MS.gene038327:CDS
GAAGAGATTGAAGAAGGACC+AGG 0.587326 5.4:-72185584 MS.gene038327:CDS
CGTGTACTTATTTACCCCAA+AGG 0.588422 5.4:-72186432 MS.gene038327:CDS
TATGCAGAAGAGATTGAAGA+AGG 0.588849 5.4:-72185590 MS.gene038327:intron
TTTCTCACAGGCTAGATGGA+AGG 0.590198 5.4:-72187490 None:intergenic
AAGGAGGTGGTGAAAATGAA+AGG 0.597643 5.4:-72187471 MS.gene038327:CDS
CTCACAGGCTAGATGGAAGG+AGG 0.605009 5.4:-72187487 None:intergenic
AACGCACGAGAAAGTGATTG+GGG 0.605913 5.4:-72186100 MS.gene038327:CDS
AAAGACATAAATCAAAATGG+AGG 0.614222 5.4:+72184782 None:intergenic
AGTTCACATTGTTCCCTTTG+GGG 0.617413 5.4:+72186418 None:intergenic
AGACATAAATCAAAATGGAG+GGG 0.626342 5.4:+72184784 None:intergenic
GGATCAGACAAATCATGAAG+AGG 0.629456 5.4:+72186061 None:intergenic
ACAGGCTAGATGGAAGGAGG+TGG 0.630158 5.4:-72187484 None:intergenic
GACTTGTTCTAATTGGTGAG+AGG 0.630409 5.4:+72185231 None:intergenic
ATCTGCAACATCCAAATACA+GGG 0.645455 5.4:+72186389 None:intergenic
CAATGTGGATGAGAAAGACA+CGG 0.658001 5.4:-72185978 MS.gene038327:CDS
CTCCTGAAGAAGTTGCTCAG+AGG 0.662017 5.4:+72185369 None:intergenic
CGTTGAAGGTCAAATTCCGG+AGG 0.683920 5.4:-72184858 MS.gene038327:CDS
TCTCACCAATTAGAACAAGT+CGG 0.697541 5.4:-72185229 MS.gene038327:CDS
ACCTGAAACTGTCAACACTG+TGG 0.702025 5.4:-72186670 MS.gene038327:CDS
TGTTAAAGTAACGCGCAATG+TGG 0.724729 5.4:-72185993 MS.gene038327:CDS
ATTTGACTTGATCATCAACG+AGG 0.752249 5.4:+72184888 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAACTGTA+AGG + chr5.4:72186012-72186031 None:intergenic 10.0%
!! GAAATTATCTAAAAATTTAG+TGG + chr5.4:72186533-72186552 None:intergenic 15.0%
!! TTAACAAATTCTAACATTTA+GGG - chr5.4:72186585-72186604 MS.gene038327:CDS 15.0%
!! TTTAACAAATTCTAACATTT+AGG - chr5.4:72186584-72186603 MS.gene038327:CDS 15.0%
!!! CTGTCAATTTTTTATTTATT+CGG - chr5.4:72186623-72186642 MS.gene038327:CDS 15.0%
!!! TGTCAATTTTTTATTTATTC+GGG - chr5.4:72186624-72186643 MS.gene038327:CDS 15.0%
!!! TTTGTTATATGATAGAAATT+TGG - chr5.4:72186431-72186450 MS.gene038327:CDS 15.0%
!! AGATATTCTTATGTACTTTA+AGG - chr5.4:72186333-72186352 MS.gene038327:CDS 20.0%
!! ATCTAAAAATTTAGTGGTTT+GGG + chr5.4:72186527-72186546 None:intergenic 20.0%
!! ATGTTCATTGTATTCATTTA+TGG + chr5.4:72184959-72184978 None:intergenic 20.0%
!! GATTTCAAATACAATTGTAT+AGG - chr5.4:72185339-72185358 MS.gene038327:CDS 20.0%
!! TAACAAATTCTAACATTTAG+GGG - chr5.4:72186586-72186605 MS.gene038327:CDS 20.0%
!! TATCTAAAAATTTAGTGGTT+TGG + chr5.4:72186528-72186547 None:intergenic 20.0%
!! TTAAACTTAAAACTTACATC+AGG + chr5.4:72186386-72186405 None:intergenic 20.0%
!! TTCATTTATTAACAACTGTA+AGG + chr5.4:72186054-72186073 None:intergenic 20.0%
!! TTGGTTGAAAAATTTAAACA+AGG - chr5.4:72186949-72186968 MS.gene038327:intron 20.0%
!!! AATTTGTTAAAATACACTAG+CGG + chr5.4:72186575-72186594 None:intergenic 20.0%
!!! AGTTTCTCTTATAGTTTTTT+AGG + chr5.4:72185169-72185188 None:intergenic 20.0%
!!! TTTTAGATAATTTCACAATC+TGG - chr5.4:72186539-72186558 MS.gene038327:intron 20.0%
! AACAAATTCTAACATTTAGG+GGG - chr5.4:72186587-72186606 MS.gene038327:CDS 25.0%
! AATAATTACACTGTGAGAAA+AGG - chr5.4:72185968-72185987 MS.gene038327:CDS 25.0%
! CAAAAATAAAGAGTCATTGA+AGG - chr5.4:72186720-72186739 MS.gene038327:intron 25.0%
! CTCTGTAATATGTAAATCAA+AGG - chr5.4:72184924-72184943 MS.gene038327:CDS 25.0%
! TATGTTGAAAAGAGAAATGA+TGG - chr5.4:72186086-72186105 MS.gene038327:CDS 25.0%
! TTTGAAAAAAGAGCAAGATT+CGG - chr5.4:72187116-72187135 MS.gene038327:intron 25.0%
!! AAAAGAGAAATGATGGAATT+AGG - chr5.4:72186093-72186112 MS.gene038327:CDS 25.0%
!! ACAATTGTATAGGTTTCTTT+TGG - chr5.4:72185349-72185368 MS.gene038327:CDS 25.0%
!! ATTCAGATGTATTTAAAGCT+GGG - chr5.4:72185687-72185706 MS.gene038327:intron 25.0%
!! CTTTGATTTACATATTACAG+AGG + chr5.4:72184926-72184945 None:intergenic 25.0%
!! TATTCAGATGTATTTAAAGC+TGG - chr5.4:72185686-72185705 MS.gene038327:intron 25.0%
!!! TCAATGACTCTTTATTTTTG+TGG + chr5.4:72186720-72186739 None:intergenic 25.0%
AAAAGAAAGACAACGAATAC+AGG - chr5.4:72184835-72184854 MS.gene038327:CDS 30.0%
ACAAATTCTAACATTTAGGG+GGG - chr5.4:72186588-72186607 MS.gene038327:CDS 30.0%
ATATATTCTCCTAAACTGCA+TGG - chr5.4:72185438-72185457 MS.gene038327:CDS 30.0%
ATCACTTACAGTAGAAAAGA+TGG - chr5.4:72185032-72185051 MS.gene038327:CDS 30.0%
ATCCTTACTATAGAATCTAC+CGG + chr5.4:72185099-72185118 None:intergenic 30.0%
ATGACAATCTTAAAGAGAGT+TGG - chr5.4:72185006-72185025 MS.gene038327:CDS 30.0%
CATAGATGAATTAAGCGTAT+AGG - chr5.4:72184976-72184995 MS.gene038327:CDS 30.0%
CTGTAAGGAACAAAATGAAT+AGG + chr5.4:72185997-72186016 None:intergenic 30.0%
TAACAACTGTAAGGAACAAT+TGG + chr5.4:72186045-72186064 None:intergenic 30.0%
TAACTTCTAAACAAAGTCAG+TGG - chr5.4:72186403-72186422 MS.gene038327:CDS 30.0%
TAGAAAAGATGGTGCAAATT+AGG - chr5.4:72185043-72185062 MS.gene038327:CDS 30.0%
TGAACTGAATATGAACAACT+GGG - chr5.4:72186653-72186672 MS.gene038327:CDS 30.0%
TGACAATCTTAAAGAGAGTT+GGG - chr5.4:72185007-72185026 MS.gene038327:CDS 30.0%
TTAAAGCTGGGGATTATAAT+TGG - chr5.4:72185699-72185718 MS.gene038327:intron 30.0%
! ACTGCATTGAAGTTATCTTT+TGG + chr5.4:72186982-72187001 None:intergenic 30.0%
! CCTTCAATGCTTCATTATTT+TGG + chr5.4:72187158-72187177 None:intergenic 30.0%
! CTCTTTTCAACATAGATGTA+TGG + chr5.4:72186080-72186099 None:intergenic 30.0%
! TACAGAATGCTGAGATTTTA+AGG + chr5.4:72186866-72186885 None:intergenic 30.0%
! TACCTGGCTTGATTAATATT+AGG + chr5.4:72186359-72186378 None:intergenic 30.0%
! TTCAGATGTATTTAAAGCTG+GGG - chr5.4:72185688-72185707 MS.gene038327:intron 30.0%
! TTCGTTGTCTTTCTTTTCTT+AGG + chr5.4:72184832-72184851 None:intergenic 30.0%
!! AATTGACAGAGTTTTATCAG+AGG - chr5.4:72186828-72186847 MS.gene038327:intron 30.0%
!! ATTGACAGAGTTTTATCAGA+GGG - chr5.4:72186829-72186848 MS.gene038327:intron 30.0%
!! CCAAAATAATGAAGCATTGA+AGG - chr5.4:72187155-72187174 MS.gene038327:intron 30.0%
!! TTTTGTGGTTTTTTTCTTCC+TGG + chr5.4:72186705-72186724 None:intergenic 30.0%
AAATCTCAGCATTCTGTACA+AGG - chr5.4:72186868-72186887 MS.gene038327:intron 35.0%
AATGCTTATCCATGCAGTTT+AGG + chr5.4:72185450-72185469 None:intergenic 35.0%
AATTAGCTGAAAACTTGGAG+AGG + chr5.4:72185525-72185544 None:intergenic 35.0%
ACATATTACAGAGGCTAAGT+AGG + chr5.4:72184917-72184936 None:intergenic 35.0%
ACTAATATAACAACTCCGAC+AGG + chr5.4:72185756-72185775 None:intergenic 35.0%
AGTAAGGATAGTAGTTCAGA+TGG - chr5.4:72185110-72185129 MS.gene038327:CDS 35.0%
ATCTGCAACATCCAAATACA+GGG + chr5.4:72185882-72185901 None:intergenic 35.0%
ATTTGTCTGATCCTGCAAAA+GGG - chr5.4:72186217-72186236 MS.gene038327:intron 35.0%
CAGTGAATTAGCTGAAAACT+TGG + chr5.4:72185530-72185549 None:intergenic 35.0%
CAGTGAGAGATACTTCAATT+AGG - chr5.4:72185400-72185419 MS.gene038327:CDS 35.0%
CTGAACTGAATATGAACAAC+TGG - chr5.4:72186652-72186671 MS.gene038327:CDS 35.0%
GAACTGAATATGAACAACTG+GGG - chr5.4:72186654-72186673 MS.gene038327:CDS 35.0%
GACAATCTTAAAGAGAGTTG+GGG - chr5.4:72185008-72185027 MS.gene038327:CDS 35.0%
GAGGATAGTTAGATCACTAA+AGG - chr5.4:72185132-72185151 MS.gene038327:CDS 35.0%
GTCAAATTGAAAACCGTTGA+AGG - chr5.4:72187396-72187415 MS.gene038327:intron 35.0%
GTGTACTTATTTACCCCAAA+GGG - chr5.4:72185837-72185856 MS.gene038327:intron 35.0%
GTTAGATCACTAAAGGTAGA+AGG - chr5.4:72185139-72185158 MS.gene038327:CDS 35.0%
TATGCAGAAGAGATTGAAGA+AGG - chr5.4:72186678-72186697 MS.gene038327:CDS 35.0%
TCTCACCAATTAGAACAAGT+CGG - chr5.4:72187039-72187058 MS.gene038327:intron 35.0%
TGAAAAACTACAAGGTGACA+GGG - chr5.4:72187278-72187297 MS.gene038327:intron 35.0%
TTCAACCAAGTCCTCTTTAA+AGG + chr5.4:72186938-72186957 None:intergenic 35.0%
TTGAAAAACTACAAGGTGAC+AGG - chr5.4:72187277-72187296 MS.gene038327:intron 35.0%
TTTGGGCAGAGTTCAAATTT+GGG + chr5.4:72186510-72186529 None:intergenic 35.0%
! AAGCCTTATCCAAGTAAGTA+GGG + chr5.4:72185284-72185303 None:intergenic 35.0%
! GAGGGAAAATAACCTAGTTT+TGG + chr5.4:72186488-72186507 None:intergenic 35.0%
! GGACAATTGACAGATTTTCT+CGG - chr5.4:72185645-72185664 MS.gene038327:intron 35.0%
! TTTCTTTTGGTATCCAAGTC+AGG - chr5.4:72185362-72185381 MS.gene038327:CDS 35.0%
!! ACATTCTCTGAAACAGTTCA+TGG - chr5.4:72185723-72185742 MS.gene038327:intron 35.0%
!! ATTTGACTTGATCATCAACG+AGG + chr5.4:72187383-72187402 None:intergenic 35.0%
!! TTGACAGAGTTTTATCAGAG+GGG - chr5.4:72186830-72186849 MS.gene038327:intron 35.0%
AACCGTTGAAGGTCAAATTC+CGG - chr5.4:72187407-72187426 MS.gene038327:intron 40.0%
AAGACACGGCTGAGAATTTA+AGG - chr5.4:72186304-72186323 MS.gene038327:CDS 40.0%
AAGGAGGTGGTGAAAATGAA+AGG - chr5.4:72184797-72184816 MS.gene038327:CDS 40.0%
AAGGATAGTAGTTCAGATGG+AGG - chr5.4:72185113-72185132 MS.gene038327:CDS 40.0%
ACAAGTTCATGAACCTGACT+TGG + chr5.4:72185378-72185397 None:intergenic 40.0%
ACAGTTCACATTGTTCCCTT+TGG + chr5.4:72185855-72185874 None:intergenic 40.0%
ACCTTCTCAACAACATTGTG+AGG - chr5.4:72187073-72187092 MS.gene038327:intron 40.0%
AGACACGGCTGAGAATTTAA+GGG - chr5.4:72186305-72186324 MS.gene038327:CDS 40.0%
AGACATGCACAGTTTAGCAT+TGG - chr5.4:72185929-72185948 MS.gene038327:intron 40.0%
AGATGGTGCAAATTAGGCTT+AGG - chr5.4:72185049-72185068 MS.gene038327:CDS 40.0%
AGTTCACATTGTTCCCTTTG+GGG + chr5.4:72185853-72185872 None:intergenic 40.0%
CAATGTGGATGAGAAAGACA+CGG - chr5.4:72186290-72186309 MS.gene038327:intron 40.0%
CAGTTCACATTGTTCCCTTT+GGG + chr5.4:72185854-72185873 None:intergenic 40.0%
CATCTGCAACATCCAAATAC+AGG + chr5.4:72185883-72185902 None:intergenic 40.0%
CGTGTACTTATTTACCCCAA+AGG - chr5.4:72185836-72185855 MS.gene038327:intron 40.0%
CTACTTACTTGGATAAGGCT+TGG - chr5.4:72185283-72185302 MS.gene038327:CDS 40.0%
GAACTGTTTGTCCCTGTATT+TGG - chr5.4:72185868-72185887 MS.gene038327:intron 40.0%
GAAGCTCTATCGAATCTTCA+AGG - chr5.4:72187458-72187477 MS.gene038327:CDS 40.0%
GACCGGTAGATTCTATAGTA+AGG - chr5.4:72185094-72185113 MS.gene038327:CDS 40.0%
GACTGTTCTTGACCAAAACT+AGG - chr5.4:72186473-72186492 MS.gene038327:intron 40.0%
GATTTGTCTGATCCTGCAAA+AGG - chr5.4:72186216-72186235 MS.gene038327:intron 40.0%
GGATCAGACAAATCATGAAG+AGG + chr5.4:72186210-72186229 None:intergenic 40.0%
GGCCTAATATTAATCAAGCC+AGG - chr5.4:72186354-72186373 MS.gene038327:CDS 40.0%
GTCTGTGAAAGAAGTCTTTG+TGG + chr5.4:72187202-72187221 None:intergenic 40.0%
GTTTGGGCAGAGTTCAAATT+TGG + chr5.4:72186511-72186530 None:intergenic 40.0%
TCCTCACAATGTTGTTGAGA+AGG + chr5.4:72187077-72187096 None:intergenic 40.0%
TCTAATTCAGTTTGAGCCTC+CGG + chr5.4:72187429-72187448 None:intergenic 40.0%
TCTGTATGTAGAGAGAAGAC+CGG - chr5.4:72185077-72185096 MS.gene038327:CDS 40.0%
TGTTAAAGTAACGCGCAATG+TGG - chr5.4:72186275-72186294 MS.gene038327:intron 40.0%
TTTGGTCAAGAACAGTCATC+AGG + chr5.4:72186470-72186489 None:intergenic 40.0%
! CAAATCATGAAGAGGCATGA+AGG + chr5.4:72186202-72186221 None:intergenic 40.0%
! CAAGCCTTATCCAAGTAAGT+AGG + chr5.4:72185285-72185304 None:intergenic 40.0%
! CAGTTACTTTGCCATATGGT+TGG - chr5.4:72185903-72185922 MS.gene038327:intron 40.0%
! GACTTGTTCTAATTGGTGAG+AGG + chr5.4:72187040-72187059 None:intergenic 40.0%
! GATGCAGTTACTTTGCCATA+TGG - chr5.4:72185899-72185918 MS.gene038327:intron 40.0%
! GTGTCACTGTTCTTTGATAC+TGG - chr5.4:72184882-72184901 MS.gene038327:CDS 40.0%
! TATCGCCTTTAAAGAGGACT+TGG - chr5.4:72186930-72186949 MS.gene038327:intron 40.0%
! TTCACAAGATACCCTTTTGC+AGG + chr5.4:72186231-72186250 None:intergenic 40.0%
!! CAACACCGACTTGTTCTAAT+TGG + chr5.4:72187047-72187066 None:intergenic 40.0%
!! GCTGACAAACTTTGAAGTGT+TGG + chr5.4:72186758-72186777 None:intergenic 40.0%
!! TTCTCTGAAACAGTTCATGG+TGG - chr5.4:72185726-72185745 MS.gene038327:intron 40.0%
!!! ACCTTGTAGTTTTTCAAGCC+TGG + chr5.4:72187274-72187293 None:intergenic 40.0%
!!! GTTTTTCAAGCCTGGCTTTA+AGG + chr5.4:72187266-72187285 None:intergenic 40.0%
!!! GTTTTTTTTCCACTCTGAGC+AGG - chr5.4:72185813-72185832 MS.gene038327:intron 40.0%
AACGCACGAGAAAGTGATTG+GGG - chr5.4:72186168-72186187 MS.gene038327:intron 45.0%
ACCTGAAACTGTCAACACTG+TGG - chr5.4:72185598-72185617 MS.gene038327:intron 45.0%
AGATTGCTGACCTTAAAGCC+AGG - chr5.4:72187253-72187272 MS.gene038327:intron 45.0%
AGCAACTTCTTCAGGAGTTG+AGG - chr5.4:72186905-72186924 MS.gene038327:intron 45.0%
AGTAAGTAGGGTCGCTTACA+TGG + chr5.4:72185272-72185291 None:intergenic 45.0%
CAACGCACGAGAAAGTGATT+GGG - chr5.4:72186167-72186186 MS.gene038327:intron 45.0%
CGACCCTACTTACTTGGATA+AGG - chr5.4:72185278-72185297 MS.gene038327:CDS 45.0%
CTCCGGAATTTGACCTTCAA+CGG + chr5.4:72187412-72187431 None:intergenic 45.0%
GAAGAGATTGAAGAAGGACC+AGG - chr5.4:72186684-72186703 MS.gene038327:intron 45.0%
GCAACTTCTTCAGGAGTTGA+GGG - chr5.4:72186906-72186925 MS.gene038327:intron 45.0%
GCCAGGCTTGAAAAACTACA+AGG - chr5.4:72187270-72187289 MS.gene038327:intron 45.0%
GGCAGAGTTCAAATTTGGGA+GGG + chr5.4:72186506-72186525 None:intergenic 45.0%
TCAACGCACGAGAAAGTGAT+TGG - chr5.4:72186166-72186185 MS.gene038327:intron 45.0%
TGGTGCAAATTAGGCTTAGG+TGG - chr5.4:72185052-72185071 MS.gene038327:CDS 45.0%
TGTAAGCGACCCTACTTACT+TGG - chr5.4:72185272-72185291 MS.gene038327:CDS 45.0%
TGTCAACACTGTGGATAGTC+AGG - chr5.4:72185607-72185626 MS.gene038327:intron 45.0%
! ACTTACATCAGGTTGCTACC+TGG + chr5.4:72186375-72186394 None:intergenic 45.0%
! GAGGGATATCGCCTTTAAAG+AGG - chr5.4:72186924-72186943 MS.gene038327:intron 45.0%
!! TCCACAGTGTTGACAGTTTC+AGG + chr5.4:72185602-72185621 None:intergenic 45.0%
AGCCTCTGAGCAACTTCTTC+AGG - chr5.4:72186897-72186916 MS.gene038327:intron 50.0%
CACTTTCTCGTGCGTTGAAC+TGG + chr5.4:72186164-72186183 None:intergenic 50.0%
CGTTGAAGGTCAAATTCCGG+AGG - chr5.4:72187410-72187429 MS.gene038327:intron 50.0%
CTCCTGAAGAAGTTGCTCAG+AGG + chr5.4:72186902-72186921 None:intergenic 50.0%
CTTGATCATCAACGAGGCGA+TGG + chr5.4:72187377-72187396 None:intergenic 50.0%
GCATGTCTGCTCCAACCATA+TGG + chr5.4:72185917-72185936 None:intergenic 50.0%
GGGCAGAGTTCAAATTTGGG+AGG + chr5.4:72186507-72186526 None:intergenic 50.0%
AAGTACACGCCTGCTCAGAG+TGG + chr5.4:72185825-72185844 None:intergenic 55.0%
GTCAGGCTGCGCGATTCAAA+TGG - chr5.4:72185624-72185643 MS.gene038327:intron 55.0%
GTTCATGGTGGTCAGCCTGT+CGG - chr5.4:72185738-72185757 MS.gene038327:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 72184793 72187497 72184793 ID=MS.gene038327
chr5.4 mRNA 72184793 72187497 72184793 ID=MS.gene038327.t1;Parent=MS.gene038327
chr5.4 exon 72187434 72187497 72187434 ID=MS.gene038327.t1.exon1;Parent=MS.gene038327.t1
chr5.4 CDS 72187434 72187497 72187434 ID=cds.MS.gene038327.t1;Parent=MS.gene038327.t1
chr5.4 exon 72186570 72186702 72186570 ID=MS.gene038327.t1.exon2;Parent=MS.gene038327.t1
chr5.4 CDS 72186570 72186702 72186570 ID=cds.MS.gene038327.t1;Parent=MS.gene038327.t1
chr5.4 exon 72186301 72186455 72186301 ID=MS.gene038327.t1.exon3;Parent=MS.gene038327.t1
chr5.4 CDS 72186301 72186455 72186301 ID=cds.MS.gene038327.t1;Parent=MS.gene038327.t1
chr5.4 exon 72185964 72186139 72185964 ID=MS.gene038327.t1.exon4;Parent=MS.gene038327.t1
chr5.4 CDS 72185964 72186139 72185964 ID=cds.MS.gene038327.t1;Parent=MS.gene038327.t1
chr5.4 exon 72184793 72185605 72184793 ID=MS.gene038327.t1.exon5;Parent=MS.gene038327.t1
chr5.4 CDS 72184793 72185605 72184793 ID=cds.MS.gene038327.t1;Parent=MS.gene038327.t1
Gene Sequence

>MS.gene038327

ATGGAAGGAGGTGGTGAAAATGAAAGGAAAACTTCTCCTAAGAAAAGAAAGACAACGAATACAGGTATTGAATGTCATCATCTTCAATAGTGTCACTGTTCTTTGATACTGGTAATATCTTCCTACTTAGCCTCTGTAATATGTAAATCAAAGGCTTACATTACCATAAATGAATACAATGAACATAGATGAATTAAGCGTATAGGATTATAAATGACAATCTTAAAGAGAGTTGGGGTATCACTTACAGTAGAAAAGATGGTGCAAATTAGGCTTAGGTGGTTTCTGTATGTAGAGAGAAGACCGGTAGATTCTATAGTAAGGATAGTAGTTCAGATGGAGGATAGTTAGATCACTAAAGGTAGAAGGAAGACCTAAAAAACTATAAGAGAAACTATTAAGAAAGATCTAGAGATTAACGAGCTAGATAGAAATATTGTATATGAATATGATAGAACATTTTGACGTCGTTTGATCCATGTAAGCGACCCTACTTACTTGGATAAGGCTTGGTTGTTGTTATTGTTGTCGTAAACATAGATGAATGATTTCAAATACAATTGTATAGGTTTCTTTTGGTATCCAAGTCAGGTTCATGAACTTGTGACAGTGAGAGATACTTCAATTAGGTTCATGAACATCAAAATATATTCTCCTAAACTGCATGGATAAGCATTAGTATCATGTACATTGTCTCGCTGCACTGTAGCACGTCTCTTAATTGTTTTACCTCTCCAAGTTTTCAGCTAATTCACTGCATGATACTTTATTTCTCTTTTCATTTATGAACATTAGCTGCTGCTCTACCTGAAACTGTCAACACTGTGGATAGTCAGGCTGCGCGATTCAAATGGACAATTGACAGATTTTCTCGGCGCATTATGAAAAATCTCTATTCAGATGTATTTAAAGCTGGGGATTATAATTGGTACATTCTCTGAAACAGTTCATGGTGGTCAGCCTGTCGGAGTTGTTATATTAGTTACAATGTGCATTTTATCTTCAGTGATATTGTCTGATGTTTTTTTTCCACTCTGAGCAGGCGTGTACTTATTTACCCCAAAGGGAACAATGTGAACTGTTTGTCCCTGTATTTGGATGTTGCAGATGCAGTTACTTTGCCATATGGTTGGAGCAGACATGCACAGTTTAGCATTGGATTAATTGATCAAATCAATAATTACACTGTGAGAAAAGGTAGCCTATTCATTTTGTTCCTTACAGTTTTTTTTTTTTTTTTTTGAACAAGCCAATTGTTCCTTACAGTTGTTAATAAATGAAGTACCATACATCTATGTTGAAAAGAGAAATGATGGAATTAGGTGTGTTATTTTTGTAACTTTATTTGCACTATGTAGAGACACAGCACCAGTTCAACGCACGAGAAAGTGATTGGGGTTTCACATCCTTCATGCCTCTTCATGATTTGTCTGATCCTGCAAAAGGGTATCTTGTGAATGATACTCTTATAGTTGATGTTGATGTTAAAGTAACGCGCAATGTGGATGAGAAAGACACGGCTGAGAATTTAAGGGTAAGTAGATATTCTTATGTACTTTAAGGCCTAATATTAATCAAGCCAGGTAGCAACCTGATGTAAGTTTTAAGTTTAACTTCTAAACAAAGTCAGTGGACTATTTTGTTATATGATAGAAATTTGGCTATTGCAATCAACCTGATGACTGTTCTTGACCAAAACTAGGTTATTTTCCCTCCCAAATTTGAACTCTGCCCAAACCACTAAATTTTTAGATAATTTCACAATCTGGAAGACTAATTCCGCTAGTGTATTTTAACAAATTCTAACATTTAGGGGGGCAGATTGAGAAACTGTCAATTTTTTATTTATTCGGGAAAATCTGAACTGAATATGAACAACTGGGGTTATGCAGAAGAGATTGAAGAAGGACCAGGAAGAAAAAAACCACAAAAATAAAGAGTCATTGAAGGAGCAAAACAAAGCCAACACTTCAAAGTTTGTCAGCAAAGACTTTGAAGAAATTGATGCTATGATAGATGTAGATGCTGTTGCTGCAATTGACAGAGTTTTATCAGAGGGGATTACTCTTTCCTTAAAATCTCAGCATTCTGTACAAGGACAAGAAGCCTCTGAGCAACTTCTTCAGGAGTTGAGGGATATCGCCTTTAAAGAGGACTTGGTTGAAAAATTTAAACAAGGTCTTTCTCCAAAAGATAACTTCAATGCAGTCAAAGAAAAAATTGACGCTAATGCTGACGTGCTTTCCTCTCACCAATTAGAACAAGTCGGTGTTGTTGTTAACCTTCTCAACAACATTGTGAGGATGTTTGAGAAACTCGAAAATTTGAAAAAAGAGCAAGATTCGGCTAAGAAGAGCACTGACCAAAATAATGAAGCATTGAAGGAAACAAGACAGAAAATATTGACCACAAAGACTTCTTTCACAGACAAACAAACACAACTTAACTCTCTTGACGTTCAGATTGCTGACCTTAAAGCCAGGCTTGAAAAACTACAAGGTGACAGGGACAAATTTGTTGAGATTCAAGATCAAGAGAAAGATAAGATCACATCTTTGAACAAAGAAGTTAAGTCAATTTTCCATCGCCTCGTTGATGATCAAGTCAAATTGAAAACCGTTGAAGGTCAAATTCCGGAGGCTCAAACTGAATTAGAAAGTCATGAGAAGCTCTATCGAATCTTCAAGGCAACCCCTCCATTTTGA
Protein sequence

>MS.gene038327.t1

MEGGGENERKTSPKKRKTTNTAAALPETVNTVDSQAARFKWTIDRFSRRIMKNLYSDVFKAGDYNWRVLIYPKGNNVNCLSLYLDVADAVTLPYGWSRHAQFSIGLIDQINNYTVRKETQHQFNARESDWGFTSFMPLHDLSDPAKGYLVNDTLIVDVDVKVTRNVDEKDTAENLRKRLKKDQEEKNHKNKESLKEQNKANTSKFVSKDFEEIDAMIDVDAVAAIDRVLSEGITLSLKSQHSVQGQEASEQLLQELRDIAFKEDLVEKFKQGLSPKDNFNAVKEKIDANADVLSSHQLEQVGVVVNLLNNIVRMFEKLENLKKEQDSAKKSTDQNNEALKETRQKILTTKTSFTDKQTQLNSLDVQIADLKARLEKLQGDRDKFVEIQDQEKDKITSLNKEVKSIFHRLVDDQVKLKTVEGQIPEAQTELESHEKLYRIFKATPPF