AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009509


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009509.t1 MTR_3g030055 80.488 164 23 2 1 164 1 155 2.38e-89 258
MS.gene009509.t1 MTR_3g030055 82.353 102 12 1 8 109 1 96 1.07e-54 169
MS.gene009509.t1 MTR_3g034200 46.584 161 69 6 13 162 2 156 2.67e-31 111
MS.gene009509.t1 MTR_3g050860 40.496 121 65 3 44 162 37 152 6.76e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009509.t1 AT5G13780 43.750 160 73 6 13 161 2 155 1.80e-27 102

Find 38 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCACAATCTGCCAATATT+CGG 0.289319 3.3:-5347932 None:intergenic
TGTTCCTTGTAACTGTTTCA+TGG 0.332180 3.3:-5347825 None:intergenic
TCCACAATCTGCCAATATTC+GGG 0.342849 3.3:-5347931 None:intergenic
GAGCTGCAGAGTTGCTCTTC+CGG 0.345662 3.3:-5348831 None:intergenic
TCTTGGTTTGTTTGAAAAGA+TGG 0.363394 3.3:+5348852 MS.gene009509:CDS
AGAGGAAGTGGAAGAGGAAG+AGG 0.396334 3.3:+5348015 MS.gene009509:CDS
CTCGTTACTCTCCCGAATAT+TGG 0.398957 3.3:+5347920 MS.gene009509:CDS
AGTGGAAGAGGAAGAGGAAG+AGG 0.420722 3.3:+5348021 MS.gene009509:CDS
CTCCTCATCATCAAAATACT+TGG 0.430558 3.3:-5348899 None:intergenic
TGCATAAGAGTCGCAACAAT+TGG 0.433948 3.3:+5347866 MS.gene009509:CDS
CTCTAAAGAGGAAGTGGAAG+AGG 0.438354 3.3:+5348009 MS.gene009509:CDS
GAAGAGGAAGAGGAAGAGGA+AGG 0.438753 3.3:+5348025 MS.gene009509:CDS
CGCCCAAAACACCATGGAAA+AGG 0.453353 3.3:+5348129 MS.gene009509:CDS
CTTGGTTTGTTTGAAAAGAT+GGG 0.459057 3.3:+5348853 MS.gene009509:CDS
CCTCCTCTCTAAAGAGGAAG+TGG 0.459131 3.3:+5348003 MS.gene009509:CDS
AACAATACAGTCGCTGCAAT+CGG 0.461342 3.3:-5347975 None:intergenic
AAGAGCAACTCTGCAGCTCT+TGG 0.462439 3.3:+5348835 MS.gene009509:CDS
TGCTTACCTCCTCTCTAAAG+AGG 0.467882 3.3:+5347997 MS.gene009509:CDS
CAATTGGAGAAGTATTAGAT+TGG 0.473450 3.3:+5347882 MS.gene009509:CDS
AAGATGTCTCGGTGCACGTC+CGG 0.505757 3.3:+5348812 MS.gene009509:CDS
CTTCCACTTCCTCTTTAGAG+AGG 0.515368 3.3:-5348006 None:intergenic
GAAGAGGAAGAGGAAGGGAA+TGG 0.522153 3.3:+5348031 MS.gene009509:CDS
CCACTTCCTCTTTAGAGAGG+AGG 0.536526 3.3:-5348003 None:intergenic
TCCCGAATATTGGCAGATTG+TGG 0.540617 3.3:+5347930 MS.gene009509:CDS
GAGTGGCTCTCACAGTTAGG+TGG 0.542114 3.3:-5348965 None:intergenic
AAGAGGAAGAGGAAGAGGAA+GGG 0.544404 3.3:+5348026 MS.gene009509:CDS
AGGAACAATGTCGTCAAAGA+AGG 0.558119 3.3:+5347841 MS.gene009509:CDS
TTACACACTTCTTGAGGTGA+AGG 0.565558 3.3:+5348876 MS.gene009509:CDS
ATAGGTATGGTGAACAAAGA+GGG 0.575631 3.3:-5348071 None:intergenic
GATGGGTTACACACTTCTTG+AGG 0.581446 3.3:+5348870 MS.gene009509:CDS
AGGAGGACGCTATTGTCTTG+TGG 0.587701 3.3:+5348917 MS.gene009509:CDS
CTAACCATGAAACAGTTACA+AGG 0.600808 3.3:+5347821 None:intergenic
GATAGGTATGGTGAACAAAG+AGG 0.607709 3.3:-5348072 None:intergenic
CACCGCCGCCCAAAACACCA+TGG 0.620119 3.3:+5348123 MS.gene009509:CDS
CACCATACCTATCGTAAACG+TGG 0.628725 3.3:+5348082 MS.gene009509:CDS
GGTGGGTGCTAAAGATGTCT+CGG 0.629911 3.3:+5348801 MS.gene009509:intron
TACCACGTTTACGATAGGTA+TGG 0.651983 3.3:-5348084 None:intergenic
AGCGATACCACGTTTACGAT+AGG 0.681782 3.3:-5348089 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTGTTTTTTGGATT+AGG + chr3.3:5348176-5348195 MS.gene009509:intron 15.0%
!!! TCTGTTATAATTTTGTTTTT+TGG + chr3.3:5348170-5348189 MS.gene009509:intron 15.0%
! ACATTGTAACAGACTATTAA+TGG - chr3.3:5348767-5348786 None:intergenic 25.0%
! CAACCAAAAACATCAAAAAA+AGG - chr3.3:5348728-5348747 None:intergenic 25.0%
! GAAAACCAAAAGAAAAATCT+CGG - chr3.3:5348633-5348652 None:intergenic 25.0%
!! TTTCTTCTTGTTAGTGATTT+TGG + chr3.3:5348550-5348569 MS.gene009509:intron 25.0%
!!! AGACCTTTTTTTGATGTTTT+TGG + chr3.3:5348722-5348741 MS.gene009509:intron 25.0%
ACAATGTGTAATTTGACTGA+AGG + chr3.3:5348780-5348799 MS.gene009509:intron 30.0%
AGAAAACAGAAAATCATCAC+AGG - chr3.3:5348379-5348398 None:intergenic 30.0%
TCCATACATAACCAGTTAAA+AGG - chr3.3:5348241-5348260 None:intergenic 30.0%
! CTAGGATTATCTTTTATTCC+TGG + chr3.3:5348422-5348441 MS.gene009509:intron 30.0%
! TATGTATGGAGTTGGTTTAA+TGG + chr3.3:5348251-5348270 MS.gene009509:intron 30.0%
! TGTGATGATTTTCTGTTTTC+TGG + chr3.3:5348378-5348397 MS.gene009509:intron 30.0%
! TTATCTTTTATTCCTGGAAG+TGG + chr3.3:5348428-5348447 MS.gene009509:intron 30.0%
!! CAATTGGAGAAGTATTAGAT+TGG + chr3.3:5347882-5347901 MS.gene009509:CDS 30.0%
!! CTTGGTTTGTTTGAAAAGAT+GGG + chr3.3:5348853-5348872 MS.gene009509:CDS 30.0%
!! TCTTGGTTTGTTTGAAAAGA+TGG + chr3.3:5348852-5348871 MS.gene009509:CDS 30.0%
!! TTTTCTTGTTTCCTAGTCTT+GGG + chr3.3:5348648-5348667 MS.gene009509:intron 30.0%
AAAACACCATGGAAAAGGTT+AGG + chr3.3:5348134-5348153 MS.gene009509:intron 35.0%
AACAAAAAACCTAGCCTACT+CGG - chr3.3:5348322-5348341 None:intergenic 35.0%
AACCAAAAGAAAAATCTCGG+AGG - chr3.3:5348630-5348649 None:intergenic 35.0%
ATAGGTATGGTGAACAAAGA+GGG - chr3.3:5348074-5348093 None:intergenic 35.0%
ATGTGTAATTTGACTGAAGG+TGG + chr3.3:5348783-5348802 MS.gene009509:intron 35.0%
CTCCTCATCATCAAAATACT+TGG - chr3.3:5348902-5348921 None:intergenic 35.0%
TAACTGGTTATGTATGGAGT+TGG + chr3.3:5348243-5348262 MS.gene009509:intron 35.0%
TAATGGTTAGGTATGAAGCT+TGG + chr3.3:5348268-5348287 MS.gene009509:intron 35.0%
TGTGTAATTTGACTGAAGGT+GGG + chr3.3:5348784-5348803 MS.gene009509:intron 35.0%
TTCCACAATCTGCCAATATT+CGG - chr3.3:5347935-5347954 None:intergenic 35.0%
! AACAAACCTAACCTTTTCCA+TGG - chr3.3:5348143-5348162 None:intergenic 35.0%
! AACCTTTTCCATGGTGTTTT+GGG - chr3.3:5348134-5348153 None:intergenic 35.0%
! ATGGAGTTGGTTTAATGGTT+AGG + chr3.3:5348256-5348275 MS.gene009509:intron 35.0%
! CTTCTTGTTAGTGATTTTGG+TGG + chr3.3:5348553-5348572 MS.gene009509:intron 35.0%
! TAACCTTTTCCATGGTGTTT+TGG - chr3.3:5348135-5348154 None:intergenic 35.0%
! TTTTCAGATAGATCACACAG+TGG - chr3.3:5348356-5348375 None:intergenic 35.0%
!! GCCTTTTAACTGGTTATGTA+TGG + chr3.3:5348237-5348256 MS.gene009509:intron 35.0%
!! GTTTTCTTGTTTCCTAGTCT+TGG + chr3.3:5348647-5348666 MS.gene009509:intron 35.0%
!! TTGTTAGTGATTTTGGTGGT+TGG + chr3.3:5348557-5348576 MS.gene009509:intron 35.0%
!! TTTCTTGTTTCCTAGTCTTG+GGG + chr3.3:5348649-5348668 MS.gene009509:intron 35.0%
!! TTTGTGTAGTTAGTCTTGTC+TGG + chr3.3:5348591-5348610 MS.gene009509:intron 35.0%
!!! CAAGTATTTTGATGATGAGG+AGG + chr3.3:5348900-5348919 MS.gene009509:CDS 35.0%
AAAATCCATACCCCAAGACT+AGG - chr3.3:5348662-5348681 None:intergenic 40.0%
AAACCAATTCTCTGTGGGAA+GGG + chr3.3:5348502-5348521 MS.gene009509:intron 40.0%
AAAGGGAGTGGCAATGATTA+GGG - chr3.3:5348468-5348487 None:intergenic 40.0%
AACAATACAGTCGCTGCAAT+CGG - chr3.3:5347978-5347997 None:intergenic 40.0%
AATCTGAGTTTCACTAGGCT+AGG + chr3.3:5348404-5348423 MS.gene009509:intron 40.0%
AGGAACAATGTCGTCAAAGA+AGG + chr3.3:5347841-5347860 MS.gene009509:CDS 40.0%
GATAGGTATGGTGAACAAAG+AGG - chr3.3:5348075-5348094 None:intergenic 40.0%
GGATTAGGTTTGTCTCTTGT+GGG + chr3.3:5348191-5348210 MS.gene009509:intron 40.0%
GGTAGAATCTGAGTTTCACT+AGG + chr3.3:5348399-5348418 MS.gene009509:intron 40.0%
GTTAGGTATGAAGCTTGGTT+CGG + chr3.3:5348273-5348292 MS.gene009509:intron 40.0%
TAAAGGGAGTGGCAATGATT+AGG - chr3.3:5348469-5348488 None:intergenic 40.0%
TACCACGTTTACGATAGGTA+TGG - chr3.3:5348087-5348106 None:intergenic 40.0%
TATTCCTGGAAGTGGTTCTT+TGG + chr3.3:5348436-5348455 MS.gene009509:intron 40.0%
TCCACAATCTGCCAATATTC+GGG - chr3.3:5347934-5347953 None:intergenic 40.0%
TGCATAAGAGTCGCAACAAT+TGG + chr3.3:5347866-5347885 MS.gene009509:CDS 40.0%
TTACACACTTCTTGAGGTGA+AGG + chr3.3:5348876-5348895 MS.gene009509:CDS 40.0%
! GTCCTCCGAGATTTTTCTTT+TGG + chr3.3:5348625-5348644 MS.gene009509:intron 40.0%
! TGGATTAGGTTTGTCTCTTG+TGG + chr3.3:5348190-5348209 MS.gene009509:intron 40.0%
! TGTGGTATAGGCCTTTTAAC+TGG + chr3.3:5348227-5348246 MS.gene009509:intron 40.0%
! TTGGGGTATGGATTTTCTCT+AGG + chr3.3:5348666-5348685 MS.gene009509:intron 40.0%
!! TTGGTTCGGTTGTTTCGTTA+GGG + chr3.3:5348287-5348306 MS.gene009509:intron 40.0%
!!! GGCCAAGTATTTTGATGATG+AGG + chr3.3:5348897-5348916 MS.gene009509:CDS 40.0%
AAACCCTTCCCACAGAGAAT+TGG - chr3.3:5348508-5348527 None:intergenic 45.0%
AAGAGGAAGAGGAAGAGGAA+GGG + chr3.3:5348026-5348045 MS.gene009509:CDS 45.0%
AGCGATACCACGTTTACGAT+AGG - chr3.3:5348092-5348111 None:intergenic 45.0%
AGGCGAAACCAATTCTCTGT+GGG + chr3.3:5348497-5348516 MS.gene009509:intron 45.0%
ATTGCCACTCCCTTTATGAG+TGG + chr3.3:5348473-5348492 MS.gene009509:intron 45.0%
CACCATACCTATCGTAAACG+TGG + chr3.3:5348082-5348101 MS.gene009509:CDS 45.0%
CTCGTTACTCTCCCGAATAT+TGG + chr3.3:5347920-5347939 MS.gene009509:CDS 45.0%
GAAACCAATTCTCTGTGGGA+AGG + chr3.3:5348501-5348520 MS.gene009509:intron 45.0%
TAGCGTATAGGGTGTGGTAT+AGG + chr3.3:5348215-5348234 MS.gene009509:intron 45.0%
TCCCGAATATTGGCAGATTG+TGG + chr3.3:5347930-5347949 MS.gene009509:CDS 45.0%
TTCACCAAAGAACCACTTCC+AGG - chr3.3:5348443-5348462 None:intergenic 45.0%
TTGCCACTCCCTTTATGAGT+GGG + chr3.3:5348474-5348493 MS.gene009509:intron 45.0%
TTTCGCCTCCCACTCATAAA+GGG - chr3.3:5348485-5348504 None:intergenic 45.0%
! CTCTAAAGAGGAAGTGGAAG+AGG + chr3.3:5348009-5348028 MS.gene009509:CDS 45.0%
! CTTCCACTTCCTCTTTAGAG+AGG - chr3.3:5348009-5348028 None:intergenic 45.0%
! GAAAAAGCTTGTTCACGTCG+CGG + chr3.3:5347952-5347971 MS.gene009509:CDS 45.0%
! TGCTTACCTCCTCTCTAAAG+AGG + chr3.3:5347997-5348016 MS.gene009509:CDS 45.0%
! TTCTCTGTGGGAAGGGTTTT+TGG + chr3.3:5348509-5348528 MS.gene009509:intron 45.0%
!! CTCTTGTGGGTTAGCGTATA+GGG + chr3.3:5348204-5348223 MS.gene009509:intron 45.0%
!! CTTGGTTCGGTTGTTTCGTT+AGG + chr3.3:5348286-5348305 MS.gene009509:intron 45.0%
!! CTTTTCCATGGTGTTTTGGG+CGG - chr3.3:5348131-5348150 None:intergenic 45.0%
!! GATGGGTTACACACTTCTTG+AGG + chr3.3:5348870-5348889 MS.gene009509:CDS 45.0%
!! TCTCTTGTGGGTTAGCGTAT+AGG + chr3.3:5348203-5348222 MS.gene009509:intron 45.0%
!! TGGTTCGGTTGTTTCGTTAG+GGG + chr3.3:5348288-5348307 MS.gene009509:intron 45.0%
!! TGTTTCCTAGTCTTGGGGTA+TGG + chr3.3:5348654-5348673 MS.gene009509:intron 45.0%
AAGAGCAACTCTGCAGCTCT+TGG + chr3.3:5348835-5348854 MS.gene009509:CDS 50.0%
AAGGTCTGTACAACAGCTGC+AGG - chr3.3:5348709-5348728 None:intergenic 50.0%
AGAGGAAGTGGAAGAGGAAG+AGG + chr3.3:5348015-5348034 MS.gene009509:CDS 50.0%
AGGAGGACGCTATTGTCTTG+TGG + chr3.3:5348917-5348936 MS.gene009509:CDS 50.0%
AGTGGAAGAGGAAGAGGAAG+AGG + chr3.3:5348021-5348040 MS.gene009509:CDS 50.0%
CGCCCAAAACACCATGGAAA+AGG + chr3.3:5348129-5348148 MS.gene009509:CDS 50.0%
GAAGAGGAAGAGGAAGAGGA+AGG + chr3.3:5348025-5348044 MS.gene009509:CDS 50.0%
GAAGAGGAAGAGGAAGGGAA+TGG + chr3.3:5348031-5348050 MS.gene009509:CDS 50.0%
GAGGCGAAACCAATTCTCTG+TGG + chr3.3:5348496-5348515 MS.gene009509:intron 50.0%
GGTGGGTGCTAAAGATGTCT+CGG + chr3.3:5348801-5348820 MS.gene009509:intron 50.0%
GTCTTGTCTGGTGAGTGTTC+TGG + chr3.3:5348603-5348622 MS.gene009509:intron 50.0%
GTTTCGCCTCCCACTCATAA+AGG - chr3.3:5348486-5348505 None:intergenic 50.0%
TACAACAGCTGCAGGTCTCA+AGG - chr3.3:5348701-5348720 None:intergenic 50.0%
TTGTTTCGTTAGGGGTGGGA+AGG + chr3.3:5348296-5348315 MS.gene009509:intron 50.0%
! CCACTTCCTCTTTAGAGAGG+AGG - chr3.3:5348006-5348025 None:intergenic 50.0%
! CCTCCTCTCTAAAGAGGAAG+TGG + chr3.3:5348003-5348022 MS.gene009509:CDS 50.0%
! TCGGTTGTTTCGTTAGGGGT+GGG + chr3.3:5348292-5348311 MS.gene009509:intron 50.0%
! TTCGGTTGTTTCGTTAGGGG+TGG + chr3.3:5348291-5348310 MS.gene009509:intron 50.0%
CCACTCCCTTTATGAGTGGG+AGG + chr3.3:5348477-5348496 MS.gene009509:intron 55.0%
CCTCCCACTCATAAAGGGAG+TGG - chr3.3:5348480-5348499 None:intergenic 55.0%
GAGCTGCAGAGTTGCTCTTC+CGG - chr3.3:5348834-5348853 None:intergenic 55.0%
! AAGATGTCTCGGTGCACGTC+CGG + chr3.3:5348812-5348831 MS.gene009509:CDS 55.0%
!! GTGGGTTAGCGTATAGGGTG+TGG + chr3.3:5348209-5348228 MS.gene009509:intron 55.0%
!! TTCCATGGTGTTTTGGGCGG+CGG - chr3.3:5348128-5348147 None:intergenic 55.0%
CACCGCCGCCCAAAACACCA+TGG + chr3.3:5348123-5348142 MS.gene009509:CDS 65.0%
GTGGGAAGGCCGAGTAGGCT+AGG + chr3.3:5348310-5348329 MS.gene009509:intron 65.0%
TAGGGGTGGGAAGGCCGAGT+AGG + chr3.3:5348305-5348324 MS.gene009509:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 5347827 5348972 5347827 ID=MS.gene009509
chr3.3 mRNA 5347827 5348972 5347827 ID=MS.gene009509.t1;Parent=MS.gene009509
chr3.3 exon 5347827 5348150 5347827 ID=MS.gene009509.t1.exon1;Parent=MS.gene009509.t1
chr3.3 CDS 5347827 5348150 5347827 ID=cds.MS.gene009509.t1;Parent=MS.gene009509.t1
chr3.3 exon 5348802 5348972 5348802 ID=MS.gene009509.t1.exon2;Parent=MS.gene009509.t1
chr3.3 CDS 5348802 5348972 5348802 ID=cds.MS.gene009509.t1;Parent=MS.gene009509.t1
Gene Sequence

>MS.gene009509

ATGAAACAGTTACAAGGAACAATGTCGTCAAAGAAGGTGTGCATAAGAGTCGCAACAATTGGAGAAGTATTAGATTGGAACAGTAACAACAATCTCGTTACTCTCCCGAATATTGGCAGATTGTGGAAAAAGCTTGTTCACGTCGCGGCCGATTGCAGCGACTGTATTGTTGCTTACCTCCTCTCTAAAGAGGAAGTGGAAGAGGAAGAGGAAGAGGAAGGGAATGGTCACTTTTGTATCGCTTCCCTCTTTGTTCACCATACCTATCGTAAACGTGGTATCGCTACTGATCTTATCACCGCCGCCCAAAACACCATGGAAAAGGTTAGGTTTGTTTCTTTATTCTGTTATAATTTTGTTTTTTGGATTAGGTTTGTCTCTTGTGGGTTAGCGTATAGGGTGTGGTATAGGCCTTTTAACTGGTTATGTATGGAGTTGGTTTAATGGTTAGGTATGAAGCTTGGTTCGGTTGTTTCGTTAGGGGTGGGAAGGCCGAGTAGGCTAGGTTTTTTGTTAATCTAGCACGCCACTGTGTGATCTATCTGAAAACCTGTGATGATTTTCTGTTTTCTGGTAGAATCTGAGTTTCACTAGGCTAGGATTATCTTTTATTCCTGGAAGTGGTTCTTTGGTGAATTCCCTAATCATTGCCACTCCCTTTATGAGTGGGAGGCGAAACCAATTCTCTGTGGGAAGGGTTTTTGGCTTCTTTTCTTCTTTCTCTTTCTTCTTGTTAGTGATTTTGGTGGTTGGTGTTTGTTGTTTTTGTGTAGTTAGTCTTGTCTGGTGAGTGTTCTGGTCCTCCGAGATTTTTCTTTTGGTTTTCTTGTTTCCTAGTCTTGGGGTATGGATTTTCTCTAGGTTCTATCTACCTTGAGACCTGCAGCTGTTGTACAGACCTTTTTTTGATGTTTTTGGTTGTCAATACATATTTTTTCCATTAATAGTCTGTTACAATGTGTAATTTGACTGAAGGTGGGTGCTAAAGATGTCTCGGTGCACGTCCGGAAGAGCAACTCTGCAGCTCTTGGTTTGTTTGAAAAGATGGGTTACACACTTCTTGAGGTGAAGGCCAAGTATTTTGATGATGAGGAGGACGCTATTGTCTTGTGGAAGAAACTCAACGAGAAGCATATAGCCACCTAA

Protein sequence

>MS.gene009509.t1

MKQLQGTMSSKKVCIRVATIGEVLDWNSNNNLVTLPNIGRLWKKLVHVAADCSDCIVAYLLSKEEVEEEEEEEGNGHFCIASLFVHHTYRKRGIATDLITAAQNTMEKVGAKDVSVHVRKSNSAALGLFEKMGYTLLEVKAKYFDDEEDAIVLWKKLNEKHIAT