AlfalfaGEDB | MS.gene036800

M. sativa cultivar XinJiangDaYe / MS.gene036800

KEGG: K12845
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005730 GO:0008150 GO:0022613 GO:0032991 GO:0042254 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0071840 GO:0097159 GO:0110165 GO:1901363 GO:1990904
PFAM: PF01248.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036800.t1	XP_003609312.1	100	128	0	0	1	128	1	128	3.90E-62	247.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036800.t1	Q5XH16	82.0	128	23	0	1	128	1	128	1.3e-53	210.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036800.t1	B7FKG0	100.0	128	0	0	1	128	1	128	2.8e-62	247.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene036800	MS.gene70551	PPI
MS.gene036800	MS.gene047734	PPI
MS.gene036800	MS.gene62426	PPI
MS.gene036800	MS.gene047337	PPI
MS.gene036800	MS.gene037324	PPI
MS.gene036800	MS.gene61498	PPI
MS.gene033348	MS.gene036800	PPI
MS.gene036800	MS.gene054186	PPI
MS.gene036800	MS.gene58148	PPI
MS.gene036800	MS.gene045843	PPI
MS.gene036800	MS.gene056257	PPI
MS.gene036800	MS.gene01057	PPI
MS.gene036800	MS.gene036487	PPI
MS.gene036800	MS.gene59319	PPI
MS.gene036800	MS.gene70292	PPI
MS.gene036800	MS.gene022249	PPI
MS.gene036800	MS.gene033017	PPI
MS.gene036800	MS.gene50744	PPI
MS.gene036800	MS.gene072782	PPI
MS.gene036800	MS.gene073888	PPI
MS.gene036800	MS.gene010453	PPI
MS.gene036800	MS.gene033349	PPI
MS.gene036800	MS.gene51687	PPI
MS.gene036800	MS.gene019081	PPI
MS.gene036800	MS.gene059422	PPI
MS.gene036800	MS.gene99744	PPI
MS.gene036800	MS.gene003693	PPI
MS.gene036800	MS.gene025347	PPI
MS.gene036800	MS.gene065462	PPI
MS.gene036800	MS.gene029242	PPI
MS.gene036800	MS.gene038800	PPI
MS.gene036800	MS.gene85342	PPI
MS.gene036800	MS.gene95934	PPI
MS.gene036800	MS.gene59047	PPI
MS.gene036800	MS.gene038837	PPI
MS.gene036800	MS.gene002484	PPI
MS.gene036800	MS.gene25903	PPI
MS.gene036800	MS.gene001986	PPI
MS.gene036800	MS.gene63427	PPI
MS.gene036800	MS.gene062116	PPI
MS.gene036800	MS.gene01456	PPI
MS.gene036800	MS.gene23314	PPI
MS.gene036800	MS.gene057157	PPI
MS.gene036800	MS.gene009509	PPI
MS.gene036800	MS.gene92650	PPI
MS.gene036800	MS.gene074400	PPI
MS.gene59047	MS.gene036800	PPI
MS.gene036800	MS.gene28172	PPI
MS.gene036800	MS.gene041557	PPI
MS.gene074400	MS.gene036800	PPI
MS.gene036800	MS.gene41916	PPI
MS.gene036800	MS.gene070139	PPI
MS.gene036800	MS.gene51967	PPI
MS.gene036800	MS.gene065463	PPI
MS.gene036800	MS.gene68979	PPI
MS.gene036800	MS.gene70579	PPI
MS.gene036800	MS.gene015586	PPI
MS.gene011390	MS.gene036800	PPI
MS.gene036800	MS.gene019309	PPI
MS.gene036800	MS.gene044498	PPI
MS.gene036800	MS.gene067362	PPI
MS.gene036800	MS.gene51964	PPI
MS.gene036800	MS.gene035331	PPI
MS.gene036800	MS.gene53517	PPI
MS.gene036800	MS.gene053443	PPI
MS.gene036800	MS.gene030697	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036800.t1	MTR_4g114330	100.000	128	0	0	1	128	1	128	1.64e-91	260
MS.gene036800.t1	MTR_0011s0330	97.656	128	3	0	1	128	1	128	3.85e-89	254
MS.gene036800.t1	MTR_4g112780	34.483	116	75	1	4	118	17	132	1.45e-17	73.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036800.t1	AT4G22380	93.750	128	8	0	1	128	1	128	1.12e-76	223
MS.gene036800.t1	AT5G20160	92.969	128	9	0	1	128	1	128	4.77e-76	221
MS.gene036800.t1	AT4G12600	89.844	128	13	0	1	128	1	128	1.26e-72	213
MS.gene036800.t1	AT5G20160	74.375	160	9	1	1	128	1	160	3.79e-69	205
MS.gene036800.t1	AT4G12600	55.738	183	26	2	1	128	1	183	2.11e-49	156
MS.gene036800.t1	AT5G20160	65.741	108	5	1	1	76	1	108	1.11e-31	108
MS.gene036800.t1	AT5G08180	35.938	128	73	1	8	126	24	151	3.66e-21	83.6
MS.gene036800.t1	AT5G08180	35.938	128	73	1	8	126	24	151	3.66e-21	83.6

Find 29 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACTATAAGCAACTCAAAA+AGG	0.236397	8.1:-12473161	MS.gene036800:CDS
TATTTCTGAATTTGTTGTTA+TGG	0.302213	8.1:-12473016	MS.gene036800:CDS
TTGTTGAACAAGATCCATTA+TGG	0.342840	8.1:+12473189	None:intergenic
TCCGTTACTTGCTGAGGATA+AGG	0.383122	8.1:-12472956	MS.gene036800:intron
AGCTACTAAGACCTTGAATA+GGG	0.384258	8.1:-12473040	MS.gene036800:CDS
TCAGCCAAAGGGTATGCCTT+AGG	0.396497	8.1:+12473229	None:intergenic
CAGCTACTAAGACCTTGAAT+AGG	0.416543	8.1:-12473041	MS.gene036800:CDS
TTCAGAAATACCCCTATTCA+AGG	0.432400	8.1:+12473029	None:intergenic
TAACCCTAAGGCATACCCTT+TGG	0.467615	8.1:-12473233	MS.gene036800:CDS
TCAGCTAACAATCACCATAA+TGG	0.473714	8.1:-12473203	MS.gene036800:CDS
CAGCCAAAGGGTATGCCTTA+GGG	0.474338	8.1:+12473230	None:intergenic
CCTCAGCAAGTAACGGAAGA+TGG	0.475355	8.1:+12472962	None:intergenic
TGTGTTCGTCACATCAAAAC+AGG	0.479560	8.1:-12472198	MS.gene036800:CDS
CCATCTTCCGTTACTTGCTG+AGG	0.512018	8.1:-12472962	MS.gene036800:CDS
GTCACATCAAAACAGGCACT+TGG	0.523031	8.1:-12472191	MS.gene036800:CDS
AGAACAAGCAATCACTGGTC+GGG	0.539786	8.1:+12472151	None:intergenic
GTCACAGAACAAGCAATCAC+TGG	0.562045	8.1:+12472146	None:intergenic
TTTGATGTGACGAACACATA+GGG	0.566367	8.1:+12472203	None:intergenic
CAGGCACTTGGGCGTGCTTG+TGG	0.575874	8.1:-12472179	MS.gene036800:CDS
TTAGCTGAGCATCAGCCAAA+GGG	0.590874	8.1:+12473218	None:intergenic
ACCTTATCCTCAGCAAGTAA+CGG	0.593594	8.1:+12472955	None:intergenic
GTTAGCTGAGCATCAGCCAA+AGG	0.598758	8.1:+12473217	None:intergenic
GTCTCAGATTCAGCAACTCA+AGG	0.599622	8.1:-12472099	MS.gene036800:intron
TGTTCTGTGACAACTAATGA+AGG	0.613805	8.1:-12472134	MS.gene036800:CDS
CAGAACAAGCAATCACTGGT+CGG	0.618383	8.1:+12472150	None:intergenic
TGGAGAAGCAGTTAACCCTA+AGG	0.621032	8.1:-12473245	MS.gene036800:CDS
TCACATCAAAACAGGCACTT+GGG	0.623134	8.1:-12472190	MS.gene036800:CDS
AGATGGAGAAGAATCTCAAG+AGG	0.644746	8.1:+12472979	None:intergenic
GCTACTAAGACCTTGAATAG+GGG	0.659558	8.1:-12473039	MS.gene036800:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAATGAATAAGAAATTA+GGG	+	chr8.1:12472213-12472232	None:intergenic	10.0%
!!	AATATTGAAATAAAATGAAT+CGG	+	chr8.1:12473034-12473053	None:intergenic	10.0%
!!	AATTTACACAATTTATAATA+AGG	-	chr8.1:12472680-12472699	MS.gene036800:intron	10.0%
!!!	ATTTTATTTCAATATTTTTC+TGG	-	chr8.1:12473038-12473057	MS.gene036800:CDS	10.0%
!!	ATTAACAAGAAGATATAAAA+CGG	+	chr8.1:12471976-12471995	None:intergenic	15.0%
!!	ATTAATCAATCAATAATGAA+AGG	+	chr8.1:12472254-12472273	None:intergenic	15.0%
!!	CAAAAAATGAATAAGAAATT+AGG	+	chr8.1:12472214-12472233	None:intergenic	15.0%
!!	CAAATTAAGAGTTAAACATA+GGG	+	chr8.1:12472003-12472022	None:intergenic	20.0%
!!	GAAAATGAATTCTGAATATT+TGG	-	chr8.1:12473327-12473346	MS.gene036800:intron	20.0%
!!	GTGTAAATTAGTATTAGTAT+AGG	+	chr8.1:12472669-12472688	None:intergenic	20.0%
!!	TCAAATTAAGAGTTAAACAT+AGG	+	chr8.1:12472004-12472023	None:intergenic	20.0%
!!!	ATTTTAGTTTTTTCATGACT+TGG	-	chr8.1:12472497-12472516	MS.gene036800:intron	20.0%
!!!	TATTTCTGAATTTGTTGTTA+TGG	-	chr8.1:12472316-12472335	MS.gene036800:intron	20.0%
!	AACTATAAGCAACTCAAAAA+GGG	-	chr8.1:12472172-12472191	MS.gene036800:CDS	25.0%
!	AGTAATATTACTTGAAGACA+TGG	+	chr8.1:12472912-12472931	None:intergenic	25.0%
!	ATAAAATGAATCGGATGAAA+TGG	+	chr8.1:12473025-12473044	None:intergenic	25.0%
!	ATGAATTCTGAATATTTGGT+TGG	-	chr8.1:12473331-12473350	MS.gene036800:intron	25.0%
!	TAACTATAAGCAACTCAAAA+AGG	-	chr8.1:12472171-12472190	MS.gene036800:CDS	25.0%
	ATGATGAAAAATTTGCAGAC+TGG	-	chr8.1:12472067-12472086	MS.gene036800:intron	30.0%
	ATGCTATGCATAACAAATAG+CGG	+	chr8.1:12472597-12472616	None:intergenic	30.0%
	TGTTATGCATAGCATCTTAT+TGG	-	chr8.1:12472602-12472621	MS.gene036800:intron	30.0%
	TTGTATCCATACAGATGTTT+GGG	-	chr8.1:12472634-12472653	MS.gene036800:intron	30.0%
	TTGTTGAACAAGATCCATTA+TGG	+	chr8.1:12472146-12472165	None:intergenic	30.0%
	TTTGTATCCATACAGATGTT+TGG	-	chr8.1:12472633-12472652	MS.gene036800:intron	30.0%
!!!	TGACTTTTAAAACAGGGTTA+TGG	-	chr8.1:12472729-12472748	MS.gene036800:intron	30.0%
	AGCTACTAAGACCTTGAATA+GGG	-	chr8.1:12472292-12472311	MS.gene036800:intron	35.0%
	CATGGTTTATCACACTTGTT+TGG	+	chr8.1:12472894-12472913	None:intergenic	35.0%
	CTTGACATTTCAAAGCAACT+AGG	+	chr8.1:12472805-12472824	None:intergenic	35.0%
	GCTGAGGATAAGGTATATTA+GGG	-	chr8.1:12472386-12472405	MS.gene036800:intron	35.0%
	TCAGCTAACAATCACCATAA+TGG	-	chr8.1:12472129-12472148	MS.gene036800:CDS	35.0%
	TCTTTCCTCTGAATGTTGTT+AGG	-	chr8.1:12472836-12472855	MS.gene036800:intron	35.0%
	TGCTATGCATAACAAATAGC+GGG	+	chr8.1:12472596-12472615	None:intergenic	35.0%
	TGCTGAGGATAAGGTATATT+AGG	-	chr8.1:12472385-12472404	MS.gene036800:intron	35.0%
	TGTTCTGTGACAACTAATGA+AGG	-	chr8.1:12473198-12473217	MS.gene036800:CDS	35.0%
	TTCAGAAATACCCCTATTCA+AGG	+	chr8.1:12472306-12472325	None:intergenic	35.0%
	TTTGATGTGACGAACACATA+GGG	+	chr8.1:12473132-12473151	None:intergenic	35.0%
!	TGAATATTTGGTTGGTATGC+AGG	-	chr8.1:12473339-12473358	MS.gene036800:intron	35.0%
!	TTTTGATGTGACGAACACAT+AGG	+	chr8.1:12473133-12473152	None:intergenic	35.0%
!!	CTAGTTGCTTTGAAATGTCA+AGG	-	chr8.1:12472803-12472822	MS.gene036800:intron	35.0%
!!!	GATCGCTGACTTTTAAAACA+GGG	-	chr8.1:12472723-12472742	MS.gene036800:intron	35.0%
!!!	TGATCGCTGACTTTTAAAAC+AGG	-	chr8.1:12472722-12472741	MS.gene036800:intron	35.0%
	ACCTTATCCTCAGCAAGTAA+CGG	+	chr8.1:12472380-12472399	None:intergenic	40.0%
	AGATGGAGAAGAATCTCAAG+AGG	+	chr8.1:12472356-12472375	None:intergenic	40.0%
	CAGCTACTAAGACCTTGAAT+AGG	-	chr8.1:12472291-12472310	MS.gene036800:intron	40.0%
	GCTACTAAGACCTTGAATAG+GGG	-	chr8.1:12472293-12472312	MS.gene036800:intron	40.0%
	GCTATGCATAACAAATAGCG+GGG	+	chr8.1:12472595-12472614	None:intergenic	40.0%
	TCACATCAAAACAGGCACTT+GGG	-	chr8.1:12473142-12473161	MS.gene036800:intron	40.0%
	TGTGTTCGTCACATCAAAAC+AGG	-	chr8.1:12473134-12473153	MS.gene036800:intron	40.0%
!!	CTCAAAAAGGGTGCTAATGA+AGG	-	chr8.1:12472184-12472203	MS.gene036800:CDS	40.0%
	AGAACAAGCAATCACTGGTC+GGG	+	chr8.1:12473184-12473203	None:intergenic	45.0%
	CAGAACAAGCAATCACTGGT+CGG	+	chr8.1:12473185-12473204	None:intergenic	45.0%
	CCACAACCCAAACATCTGTA+TGG	+	chr8.1:12472643-12472662	None:intergenic	45.0%
	CGAGACCTAACAACATTCAG+AGG	+	chr8.1:12472844-12472863	None:intergenic	45.0%
	GTCACAGAACAAGCAATCAC+TGG	+	chr8.1:12473189-12473208	None:intergenic	45.0%
	GTCACATCAAAACAGGCACT+TGG	-	chr8.1:12473141-12473160	MS.gene036800:intron	45.0%
	GTCTCAGATTCAGCAACTCA+AGG	-	chr8.1:12473233-12473252	MS.gene036800:CDS	45.0%
	TAACCCTAAGGCATACCCTT+TGG	-	chr8.1:12472099-12472118	MS.gene036800:intron	45.0%
	TGGAGAAGCAGTTAACCCTA+AGG	-	chr8.1:12472087-12472106	MS.gene036800:intron	45.0%
	TTAGCTGAGCATCAGCCAAA+GGG	+	chr8.1:12472117-12472136	None:intergenic	45.0%
!	TCCGTTACTTGCTGAGGATA+AGG	-	chr8.1:12472376-12472395	MS.gene036800:intron	45.0%
!!	CCATACAGATGTTTGGGTTG+TGG	-	chr8.1:12472640-12472659	MS.gene036800:intron	45.0%
	GTTAGCTGAGCATCAGCCAA+AGG	+	chr8.1:12472118-12472137	None:intergenic	50.0%
!	CAGCCAAAGGGTATGCCTTA+GGG	+	chr8.1:12472105-12472124	None:intergenic	50.0%
!	CCATCTTCCGTTACTTGCTG+AGG	-	chr8.1:12472370-12472389	MS.gene036800:intron	50.0%
!	CCTCAGCAAGTAACGGAAGA+TGG	+	chr8.1:12472373-12472392	None:intergenic	50.0%
!	TCAGCCAAAGGGTATGCCTT+AGG	+	chr8.1:12472106-12472125	None:intergenic	50.0%
	CAGGCACTTGGGCGTGCTTG+TGG	-	chr8.1:12473153-12473172	MS.gene036800:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	12471967	12473387	12471967	ID=MS.gene036800
chr8.1	mRNA	12471967	12473387	12471967	ID=MS.gene036800.t1;Parent=MS.gene036800
chr8.1	exon	12473385	12473387	12473385	ID=MS.gene036800.t1.exon1;Parent=MS.gene036800.t1
chr8.1	CDS	12473385	12473387	12473385	ID=cds.MS.gene036800.t1;Parent=MS.gene036800.t1
chr8.1	exon	12473149	12473269	12473149	ID=MS.gene036800.t1.exon2;Parent=MS.gene036800.t1
chr8.1	CDS	12473149	12473269	12473149	ID=cds.MS.gene036800.t1;Parent=MS.gene036800.t1
chr8.1	exon	12472957	12473060	12472957	ID=MS.gene036800.t1.exon3;Parent=MS.gene036800.t1
chr8.1	CDS	12472957	12473060	12472957	ID=cds.MS.gene036800.t1;Parent=MS.gene036800.t1
chr8.1	exon	12472100	12472231	12472100	ID=MS.gene036800.t1.exon4;Parent=MS.gene036800.t1
chr8.1	CDS	12472100	12472231	12472100	ID=cds.MS.gene036800.t1;Parent=MS.gene036800.t1
chr8.1	exon	12471967	12471993	12471967	ID=MS.gene036800.t1.exon5;Parent=MS.gene036800.t1
chr8.1	CDS	12471967	12471993	12471967	ID=cds.MS.gene036800.t1;Parent=MS.gene036800.t1

Gene Sequence

>MS.gene036800

ATGGTACCGTTTTATATCTTCTTGTTAATTTTTCCCTATGTTTAACTCTTAATTTGATTCAGTGTTAATTGATTAATGATTAATACTTGTTTTTTTGTATATGATGAAAAATTTGCAGACTGGAGAAGCAGTTAACCCTAAGGCATACCCTTTGGCTGATGCTCAGCTAACAATCACCATAATGGATCTTGTTCAACAAGCTGCTAACTATAAGCAACTCAAAAAGGGTGCTAATGAAGGTAACCCTAATTTCTTATTCATTTTTTGTTTAATGTCTTTTTTGTCCTTTCATTATTGATTGATTAATTGATTTTTGTTGTGTTGCAGCTACTAAGACCTTGAATAGGGGTATTTCTGAATTTGTTGTTATGGCTGCTGATGCTGAACCTCTTGAGATTCTTCTCCATCTTCCGTTACTTGCTGAGGATAAGGTATATTAGGGTTTTGTTTATGTAATGTATGATCTTCATATAATGAAATGAAATGACTAGCTATGTTAACTAAATCTGTCACAAATTAAAAGTTAAACAATTTTAGTTTTTTCATGACTTGGAAATAAATGTTTTATTTGTGAATATATATGATTATGCTATGCTTTTTCGTAGTTCACAAACAAAGAAATCGACCCCGCTATTTGTTATGCATAGCATCTTATTGGTACAATCATTTGTATCCATACAGATGTTTGGGTTGTGGTCTCCTATACTAATACTAATTTACACAATTTATAATAAGGCTGTAATTGCATTTGCTGCTGATCGCTGACTTTTAAAACAGGGTTATGGTATGTTTGTGTTATGATATGTTTATGTAGCTTATTGTTATTGTTATGTAGCCTAGTTGCTTTGAAATGTCAAGGTTGTGAAATTTCTTTCCTCTGAATGTTGTTAGGTCTCGATGAGTTTGTTGTAACTTACTCATAAACCAAACAAGTGTGATAAACCATGTCTTCAAGTAATATTACTATGAACTGTCACAGTATCAGTATAGTTTTTGTCTCTGCTAATCTTTCTTGCGTGTGTTGCATTGCAATTTGTAAATGTATTTTGTCTTAGCCATTTCATCCGATTCATTTTATTTCAATATTTTTCTGGTTTTTGTCTATCAGCAATGTATAGATTATTAATCATGTGCACTATCTGTTTGAAATTTTTAGAATGTTCCCTATGTGTTCGTCACATCAAAACAGGCACTTGGGCGTGCTTGTGGTGTCACCCGACCAGTGATTGCTTGTTCTGTGACAACTAATGAAGGTAGTCAATTGAAGTCTCAGATTCAGCAACTCAAGGTATGCACTATGCTTCGTTTTCATGATTTTCATTTTCACTATTTATTGTAATTGTTATATGTTACTTTAATTGAAAATGAATTCTGAATATTTGGTTGGTATGCAGGATGCTATTGAGAAGCTGCTAATCTGA

Protein sequence

>MS.gene036800.t1

MTGEAVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVTSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI