AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045843

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045843.t1 MTR_0011s0330 100.000 128 0 0 1 128 1 128 9.70e-92 261
MS.gene045843.t1 MTR_4g114330 97.656 128 3 0 1 128 1 128 3.85e-89 254
MS.gene045843.t1 MTR_4g112780 34.821 112 72 1 8 118 21 132 1.18e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045843.t1 AT4G22380 93.750 128 8 0 1 128 1 128 2.75e-77 224
MS.gene045843.t1 AT5G20160 92.969 128 9 0 1 128 1 128 1.31e-76 223
MS.gene045843.t1 AT4G12600 91.406 128 11 0 1 128 1 128 2.62e-74 217
MS.gene045843.t1 AT5G20160 74.375 160 9 1 1 128 1 160 6.71e-70 207
MS.gene045843.t1 AT4G12600 56.284 183 25 2 1 128 1 183 1.76e-49 156
MS.gene045843.t1 AT5G20160 64.815 108 6 1 1 76 1 108 6.22e-31 107
MS.gene045843.t1 AT5G08180 36.641 131 74 1 5 126 21 151 3.54e-23 88.6
MS.gene045843.t1 AT5G08180 36.641 131 74 1 5 126 21 151 3.54e-23 88.6

Find 24 sgRNAs with CRISPR-Local

Find 275 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCCAAAGGGTAAGCTTT+TGG 0.274630 2.4:+34827058 None:intergenic
TAATTACAAGCAACTCAAAA+AGG 0.282256 2.4:-34826990 MS.gene045843:CDS
TCCTTTACTCGCTGAGGATA+AGG 0.365424 2.4:-34826714 MS.gene045843:intron
AATCAAAAGCACACCTTCAT+TGG 0.389057 2.4:+34826964 None:intergenic
ACAACTTACGATAACGATAA+TGG 0.396702 2.4:-34827032 MS.gene045843:CDS
TTTAAGTTGACTTCCCTCAT+TGG 0.429117 2.4:+34823638 None:intergenic
CATTTCTGAGTTTGTTGTGA+TGG 0.445083 2.4:-34826774 MS.gene045843:CDS
TTTGATGGGACAAATACATA+AGG 0.470260 2.4:+34823720 None:intergenic
GAATCCAAAAGCTTACCCTT+TGG 0.476923 2.4:-34827062 MS.gene045843:CDS
ATCTCAAATACAACAACTAA+AGG 0.485983 2.4:-34823616 MS.gene045843:intron
GTCACAGAACATGCAATCAC+TGG 0.528661 2.4:+34823663 None:intergenic
ACCTTATCCTCAGCGAGTAA+AGG 0.534236 2.4:+34826713 None:intergenic
CAGAACATGCAATCACTGGC+CGG 0.535766 2.4:+34823667 None:intergenic
GTCCCATCAAAACAAGCACT+TGG 0.547834 2.4:-34823708 MS.gene045843:CDS
TCATCTTCCTTTACTCGCTG+AGG 0.553114 2.4:-34826720 MS.gene045843:CDS
GACGAGCATGTGGTGTGACC+CGG 0.557034 2.4:-34823686 MS.gene045843:CDS
TAAGTTGTGCATCAGCCAAA+GGG 0.569151 2.4:+34827047 None:intergenic
AGAACATGCAATCACTGGCC+GGG 0.572135 2.4:+34823668 None:intergenic
GTAAGTTGTGCATCAGCCAA+AGG 0.580950 2.4:+34827046 None:intergenic
CAAGCACTTGGACGAGCATG+TGG 0.613075 2.4:-34823696 MS.gene045843:CDS
TGTTCTGTGACAACCAATGA+GGG 0.643609 2.4:-34823651 MS.gene045843:CDS
AGATGAAGAAGAATCTCAAG+TGG 0.658274 2.4:+34826737 None:intergenic
ATGTTCTGTGACAACCAATG+AGG 0.691643 2.4:-34823652 MS.gene045843:CDS
GCTACAAAGACACTTAACAG+AGG 0.744755 2.4:-34826797 MS.gene045843:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAAAAATAAAAAAAT+AGG + chr2.4:34824007-34824026 None:intergenic 0.0%
!!! TATTTTTTATTTTTTTTTAT+GGG - chr2.4:34824110-34824129 MS.gene045843:intron 0.0%
!!! TTATTTTTTATTTTTTTTTA+TGG - chr2.4:34824109-34824128 MS.gene045843:intron 0.0%
!! AATAATTCAAAAAAATGAAT+AGG + chr2.4:34823521-34823540 None:intergenic 10.0%
!!! ATATCAATTTTATATTACAA+TGG - chr2.4:34825783-34825802 MS.gene045843:intron 10.0%
!!! TATCAATTTTATATTACAAT+GGG - chr2.4:34825784-34825803 MS.gene045843:intron 10.0%
!!! TATTAAAATGATTTATGTTT+TGG - chr2.4:34824660-34824679 MS.gene045843:intron 10.0%
!! ACAAAAATAATCATAATTCA+AGG - chr2.4:34825239-34825258 MS.gene045843:intron 15.0%
!! AGTATTAATTAGAGTTATAA+AGG + chr2.4:34826497-34826516 None:intergenic 15.0%
!! GTATTAATTAGAGTTATAAA+GGG + chr2.4:34826496-34826515 None:intergenic 15.0%
!! TATTAATTAGAGTTATAAAG+GGG + chr2.4:34826495-34826514 None:intergenic 15.0%
!! TGAAAATTCAAATAATTACA+TGG + chr2.4:34826924-34826943 None:intergenic 15.0%
!! TGTATTCTATTTAATTAGTT+TGG - chr2.4:34824860-34824879 MS.gene045843:intron 15.0%
!!! ATTTTTATTTTCAGTTTCTA+TGG - chr2.4:34823750-34823769 MS.gene045843:intron 15.0%
!!! CATTGAATTTCTAATTTTTT+TGG + chr2.4:34825114-34825133 None:intergenic 15.0%
!!! TAATTGAATTCTGATTATTT+TGG - chr2.4:34824598-34824617 MS.gene045843:intron 15.0%
!!! TATTTTAATTGAAAAATTGG+TGG - chr2.4:34825708-34825727 MS.gene045843:intron 15.0%
!!! TTTAATGGAAATTGTTTTAT+TGG - chr2.4:34826137-34826156 MS.gene045843:intron 15.0%
!! AAAAAGAAAAAGTGGTATTA+GGG - chr2.4:34823775-34823794 MS.gene045843:intron 20.0%
!! AAAGTGGAATATATTGAATT+GGG + chr2.4:34826532-34826551 None:intergenic 20.0%
!! ACTTGATAAGAAATTTGATA+AGG + chr2.4:34826228-34826247 None:intergenic 20.0%
!! ATAAGAAATTTGATAAGGTA+AGG + chr2.4:34826223-34826242 None:intergenic 20.0%
!! ATTATAACAGCTAATAAAGT+TGG + chr2.4:34827119-34827138 None:intergenic 20.0%
!! TAAAAAGAAAAAGTGGTATT+AGG - chr2.4:34823774-34823793 MS.gene045843:intron 20.0%
!! TAAAGTCTAAACAAATCAAA+GGG + chr2.4:34824495-34824514 None:intergenic 20.0%
!! TTAAAGTCTAAACAAATCAA+AGG + chr2.4:34824496-34824515 None:intergenic 20.0%
!! TTAATTTATGACAGTTGAAA+AGG - chr2.4:34826891-34826910 MS.gene045843:intron 20.0%
!! TTATCAAGTAATATGTGTAA+TGG - chr2.4:34826239-34826258 MS.gene045843:intron 20.0%
!!! AAATTGTTTTATTGGTAAGT+TGG - chr2.4:34826145-34826164 MS.gene045843:intron 20.0%
!!! GACACTTTTTTTTTGATAAA+TGG + chr2.4:34826457-34826476 None:intergenic 20.0%
! AAATAAATGAGAGAGAAAAG+TGG + chr2.4:34826585-34826604 None:intergenic 25.0%
! AATAGGCAATTAACATTTGT+TGG + chr2.4:34823990-34824009 None:intergenic 25.0%
! AATTACAAGCAACTCAAAAA+GGG - chr2.4:34823682-34823701 MS.gene045843:CDS 25.0%
! AGTATATGTATAAGACAATG+CGG + chr2.4:34826411-34826430 None:intergenic 25.0%
! ATAAATGGCTCAGTTATTAA+GGG + chr2.4:34826442-34826461 None:intergenic 25.0%
! ATATGTGTGAAATGAGAAAA+TGG - chr2.4:34826619-34826638 MS.gene045843:intron 25.0%
! ATCTCAAATACAACAACTAA+AGG - chr2.4:34827055-34827074 MS.gene045843:CDS 25.0%
! ATTTGAGGGATTTAAAAATG+AGG - chr2.4:34825851-34825870 MS.gene045843:intron 25.0%
! CAAAAAAATGAATAGGAGAA+AGG + chr2.4:34823514-34823533 None:intergenic 25.0%
! CAAAGTGGAATATATTGAAT+TGG + chr2.4:34826533-34826552 None:intergenic 25.0%
! CTATGGTTAAAAAGAAAAAG+TGG - chr2.4:34823767-34823786 MS.gene045843:intron 25.0%
! GAAAAACTTTAGATTTGTCT+TGG + chr2.4:34826737-34826756 None:intergenic 25.0%
! GAATAATATCAAGCAAAACA+TGG + chr2.4:34827087-34827106 None:intergenic 25.0%
! GAATGAAGAAAATTGAGAAA+CGG + chr2.4:34823553-34823572 None:intergenic 25.0%
! GATTATTAACCAGATTGTAA+TGG + chr2.4:34825178-34825197 None:intergenic 25.0%
! TAATATCATCAACACTAAAG+TGG - chr2.4:34825198-34825217 MS.gene045843:intron 25.0%
! TAATTACAAGCAACTCAAAA+AGG - chr2.4:34823681-34823700 MS.gene045843:CDS 25.0%
! TAGTAAAGAGCTTAGAAATT+TGG - chr2.4:34825140-34825159 MS.gene045843:intron 25.0%
! TCCTGTATGAAAAAATAGTT+TGG - chr2.4:34824889-34824908 MS.gene045843:intron 25.0%
! TTTCTATTCATTCATGTTCT+TGG - chr2.4:34826864-34826883 MS.gene045843:intron 25.0%
!! ATTTGGCATATATTGGTAAT+AGG + chr2.4:34826796-34826815 None:intergenic 25.0%
!! CTTTTTATCAAGTTTCTTTG+CGG - chr2.4:34826195-34826214 MS.gene045843:intron 25.0%
!! TAAAGGGGAAAAAGTTATTT+TGG + chr2.4:34826480-34826499 None:intergenic 25.0%
!! TCATTGAACTCTGATTATTT+TGG - chr2.4:34824713-34824732 MS.gene045843:intron 25.0%
!!! AAAGGGGAAAAAGTTATTTT+GGG + chr2.4:34826479-34826498 None:intergenic 25.0%
!!! AAATATGTCTGTGTTTTTGA+AGG - chr2.4:34824024-34824043 MS.gene045843:intron 25.0%
!!! AATATGTCTGTGTTTTTGAA+GGG - chr2.4:34824025-34824044 MS.gene045843:intron 25.0%
!!! TCCAAACTATTTTTTCATAC+AGG + chr2.4:34824893-34824912 None:intergenic 25.0%
!!! TCCATGTCTTTTTAAAATTG+TGG - chr2.4:34826676-34826695 MS.gene045843:intron 25.0%
!!! TTTTATTGGTAAGTTGGTAA+CGG - chr2.4:34826151-34826170 MS.gene045843:intron 25.0%
!!! TTTTTTTTTTTGTTGCAGAC+AGG - chr2.4:34823577-34823596 MS.gene045843:intron 25.0%
AAAGTTATACAGTAACCCAA+AGG + chr2.4:34825743-34825762 None:intergenic 30.0%
AAGTGCTCTTGATAAGTATT+TGG + chr2.4:34826558-34826577 None:intergenic 30.0%
AATGTGGATAGAGATTTAGA+AGG - chr2.4:34824236-34824255 MS.gene045843:intron 30.0%
ACAACTTACGATAACGATAA+TGG - chr2.4:34823639-34823658 MS.gene045843:CDS 30.0%
AGAAAATGGCTCAGTTAATT+TGG - chr2.4:34826633-34826652 MS.gene045843:intron 30.0%
ATACAAACAAAACAGACCAA+AGG - chr2.4:34824948-34824967 MS.gene045843:intron 30.0%
GAAAATGGCTCAGTTAATTT+GGG - chr2.4:34826634-34826653 MS.gene045843:intron 30.0%
GATAAATGGCTCAGTTATTA+AGG + chr2.4:34826443-34826462 None:intergenic 30.0%
GGCATTACATAATTTCAACA+TGG + chr2.4:34826087-34826106 None:intergenic 30.0%
GGTTAAAGTAATATGTGTAG+TGG - chr2.4:34826172-34826191 MS.gene045843:intron 30.0%
GTGTGATCGTTGAAAATTTA+AGG + chr2.4:34826382-34826401 None:intergenic 30.0%
TAACGTAGACATAATCACTA+TGG - chr2.4:34824513-34824532 MS.gene045843:intron 30.0%
TACAAACAAAACAGACCAAA+GGG - chr2.4:34824949-34824968 MS.gene045843:intron 30.0%
TAGTTTAGATTCGTTTCTTG+TGG - chr2.4:34825895-34825914 MS.gene045843:intron 30.0%
TCAGTCAATTTGGCATATAT+TGG + chr2.4:34826803-34826822 None:intergenic 30.0%
TCATCTTATCAACACATTAG+TGG - chr2.4:34825073-34825092 MS.gene045843:intron 30.0%
TGAATGAATAGAAATAGCTG+TGG + chr2.4:34826858-34826877 None:intergenic 30.0%
TGTTATAATGCCAACACATT+TGG - chr2.4:34827130-34827149 MS.gene045843:intron 30.0%
TTGTTCATATATAAGCCCTT+TGG + chr2.4:34824967-34824986 None:intergenic 30.0%
TTTGATGGGACAAATACATA+AGG + chr2.4:34826954-34826973 None:intergenic 30.0%
! AAGATTTCCTTTTCCTTTTG+TGG + chr2.4:34825987-34826006 None:intergenic 30.0%
! TTTGGGTTACTGTATAACTT+TGG - chr2.4:34825742-34825761 MS.gene045843:intron 30.0%
!! ACTGTATAACTTTGGTTCTT+TGG - chr2.4:34825750-34825769 MS.gene045843:intron 30.0%
!! CTGTATAACTTTGGTTCTTT+GGG - chr2.4:34825751-34825770 MS.gene045843:intron 30.0%
!! GCCACAATTTTAAAAAGACA+TGG + chr2.4:34826680-34826699 None:intergenic 30.0%
!! TAGAAAGTATTTATGAGCCT+GGG - chr2.4:34824259-34824278 MS.gene045843:intron 30.0%
!!! GTCTTTTTAAAATTGTGGCT+AGG - chr2.4:34826681-34826700 MS.gene045843:intron 30.0%
AAAAACGAGCCATTACAATC+TGG - chr2.4:34825166-34825185 MS.gene045843:intron 35.0%
AAACGAAACAGACACAAGAA+AGG + chr2.4:34824841-34824860 None:intergenic 35.0%
AACGAAACAGACACAAGAAA+GGG + chr2.4:34824840-34824859 None:intergenic 35.0%
AGATGAAGAAGAATCTCAAG+TGG + chr2.4:34823937-34823956 None:intergenic 35.0%
AGCAGCAATAACAGTAACAA+TGG + chr2.4:34824079-34824098 None:intergenic 35.0%
AGTCTGGAAAAAACAGAACA+GGG + chr2.4:34826831-34826850 None:intergenic 35.0%
CAAAAAACCAACAGAAGGAA+TGG + chr2.4:34824292-34824311 None:intergenic 35.0%
CCTCAAATGCATTAACATCA+GGG + chr2.4:34825839-34825858 None:intergenic 35.0%
CCTGATGTTAATGCATTTGA+GGG - chr2.4:34825837-34825856 MS.gene045843:intron 35.0%
CGATGTCCATACAAATCAAA+AGG - chr2.4:34825283-34825302 MS.gene045843:intron 35.0%
GCAAAAACCAATAGATGTAG+TGG - chr2.4:34825489-34825508 MS.gene045843:intron 35.0%
GTAGAAAGGATTTACGAGTT+TGG + chr2.4:34825522-34825541 None:intergenic 35.0%
TAGTCTGGAAAAAACAGAAC+AGG + chr2.4:34826832-34826851 None:intergenic 35.0%
TCAAGAATGTCCAAATGTGT+TGG + chr2.4:34827143-34827162 None:intergenic 35.0%
TCCTTCAAAAAACCAACAGA+AGG + chr2.4:34824297-34824316 None:intergenic 35.0%
TGAATAGGAGAAAGGAAACA+AGG + chr2.4:34823506-34823525 None:intergenic 35.0%
TGATTTATGACTAGTGCAAC+TGG - chr2.4:34823805-34823824 MS.gene045843:intron 35.0%
TGGAGATTCATAAGGTAGAA+AGG + chr2.4:34825536-34825555 None:intergenic 35.0%
TTGTCATCTTGATGCTCTTT+GGG + chr2.4:34825311-34825330 None:intergenic 35.0%
TTTGGACATTCTTGATATGC+AGG - chr2.4:34827148-34827167 MS.gene045843:intron 35.0%
! AATCAAAAGCACACCTTCAT+TGG + chr2.4:34823710-34823729 None:intergenic 35.0%
! CTTTGGGAGGGATTATTTAA+TGG - chr2.4:34826122-34826141 MS.gene045843:intron 35.0%
! GGCATATATTGGTAATAGGT+CGG + chr2.4:34826792-34826811 None:intergenic 35.0%
!! AAAGAGCATCAAGATGACAA+GGG - chr2.4:34825311-34825330 MS.gene045843:intron 35.0%
!! CATTTCTGAGTTTGTTGTGA+TGG - chr2.4:34823897-34823916 MS.gene045843:intron 35.0%
!! CGTGTTAGTTTACTGCATTT+TGG + chr2.4:34825378-34825397 None:intergenic 35.0%
!! GAGTACTGAGCTTAGAAATT+TGG + chr2.4:34824686-34824705 None:intergenic 35.0%
!! GATAAGTATTTGGTGCAAAG+TGG + chr2.4:34826548-34826567 None:intergenic 35.0%
!! GTAGAAAGTATTTATGAGCC+TGG - chr2.4:34824258-34824277 MS.gene045843:intron 35.0%
!! TAGTTTACTGCATTTTGGTC+TGG + chr2.4:34825373-34825392 None:intergenic 35.0%
!! TTTAAGTTGACTTCCCTCAT+TGG + chr2.4:34827036-34827055 None:intergenic 35.0%
!!! ACTGGCAAAAAGATTGGTTT+TGG + chr2.4:34826323-34826342 None:intergenic 35.0%
!!! CTGGCAAAAAGATTGGTTTT+GGG + chr2.4:34826322-34826341 None:intergenic 35.0%
!!! TCCTTCTGTTGGTTTTTTGA+AGG - chr2.4:34824293-34824312 MS.gene045843:intron 35.0%
!!! TGGCAAAAAGATTGGTTTTG+GGG + chr2.4:34826321-34826340 None:intergenic 35.0%
!!! TGGGAACCTTTTGATTTGTA+TGG + chr2.4:34825292-34825311 None:intergenic 35.0%
!!! TTGGTTTTTTGAAGGACATG+TGG - chr2.4:34824301-34824320 MS.gene045843:intron 35.0%
AAACTCTAATGCATACCGCT+TGG + chr2.4:34826037-34826056 None:intergenic 40.0%
AACTCTAATGCATACCGCTT+GGG + chr2.4:34826036-34826055 None:intergenic 40.0%
AAGGTGGATGGAGATTCATA+AGG + chr2.4:34825544-34825563 None:intergenic 40.0%
AATCACTGGTGGAACCAATA+TGG - chr2.4:34824332-34824351 MS.gene045843:intron 40.0%
ACTAGTGCAACTGGATTGAA+TGG - chr2.4:34823814-34823833 MS.gene045843:intron 40.0%
ATCTCTGCCACTACATCTAT+TGG + chr2.4:34825499-34825518 None:intergenic 40.0%
ATGGCTCAGTTATTAAGGGA+CGG + chr2.4:34826438-34826457 None:intergenic 40.0%
ATGTTCTGTGACAACCAATG+AGG - chr2.4:34827019-34827038 MS.gene045843:CDS 40.0%
CACTATGGAAAGACACTACT+AGG - chr2.4:34824528-34824547 MS.gene045843:intron 40.0%
CAGGGGAATATCAGTCAATT+TGG + chr2.4:34826813-34826832 None:intergenic 40.0%
CCCTCAAATGCATTAACATC+AGG + chr2.4:34825840-34825859 None:intergenic 40.0%
CCCTGATGTTAATGCATTTG+AGG - chr2.4:34825836-34825855 MS.gene045843:intron 40.0%
CCTAAACCAATTCTCAACCA+TGG + chr2.4:34824441-34824460 None:intergenic 40.0%
CGTCTCATCAAACATGTAAG+TGG + chr2.4:34824636-34824655 None:intergenic 40.0%
CTTCCCACTTTGTGAACTTT+GGG - chr2.4:34826106-34826125 MS.gene045843:intron 40.0%
CTTGTCATCTTGATGCTCTT+TGG + chr2.4:34825312-34825331 None:intergenic 40.0%
GAATCCAAAAGCTTACCCTT+TGG - chr2.4:34823609-34823628 MS.gene045843:intron 40.0%
GATTCGTTTCTTGTGGAAAG+TGG - chr2.4:34825902-34825921 MS.gene045843:intron 40.0%
GCTACAAAGACACTTAACAG+AGG - chr2.4:34823874-34823893 MS.gene045843:intron 40.0%
GGTAAACCATGGTTGAGAAT+TGG - chr2.4:34824432-34824451 MS.gene045843:intron 40.0%
GTCTGGAAAAAACAGAACAG+GGG + chr2.4:34826830-34826849 None:intergenic 40.0%
GTCTGGTTAGATTATTGCGT+TGG - chr2.4:34824400-34824419 MS.gene045843:intron 40.0%
TAAGTTGTGCATCAGCCAAA+GGG + chr2.4:34823627-34823646 None:intergenic 40.0%
TAGCGAACCACAAAAGGAAA+AGG - chr2.4:34825977-34825996 MS.gene045843:intron 40.0%
TGGTGGAACCAATATGGTTT+GGG - chr2.4:34824338-34824357 MS.gene045843:intron 40.0%
TGTCGTGATTGCAAACATGT+TGG + chr2.4:34825936-34825955 None:intergenic 40.0%
TGTTCTGTGACAACCAATGA+GGG - chr2.4:34827020-34827039 MS.gene045843:CDS 40.0%
TTTATGGGGCAATGTTTGAG+AGG - chr2.4:34824125-34824144 MS.gene045843:intron 40.0%
TTTCAACATGGCATACATGC+AGG + chr2.4:34826075-34826094 None:intergenic 40.0%
! ACCAATCTTTTTGCCAGTAG+CGG - chr2.4:34826325-34826344 MS.gene045843:intron 40.0%
! ATGGCTCAGTTAATTTGGGA+CGG - chr2.4:34826638-34826657 MS.gene045843:intron 40.0%
! CCATGGTTGAGAATTGGTTT+AGG - chr2.4:34824438-34824457 MS.gene045843:intron 40.0%
! GAAAAAAAGGGTACATGGAG+AGG + chr2.4:34825572-34825591 None:intergenic 40.0%
! GGTGAAGTAGCGGAAAAAAA+GGG + chr2.4:34825584-34825603 None:intergenic 40.0%
! TAGCGGAAAAAAAGGGTACA+TGG + chr2.4:34825577-34825596 None:intergenic 40.0%
! TGGTGAAGTAGCGGAAAAAA+AGG + chr2.4:34825585-34825604 None:intergenic 40.0%
! TTATTGCGTTGGTCTGTTAG+TGG - chr2.4:34824411-34824430 MS.gene045843:intron 40.0%
!! AAAGCTTGCTACTTCAACAC+TGG + chr2.4:34825625-34825644 None:intergenic 40.0%
!! AAGCTTGCTACTTCAACACT+GGG + chr2.4:34825624-34825643 None:intergenic 40.0%
!! CAAAGAGCATCAAGATGACA+AGG - chr2.4:34825310-34825329 MS.gene045843:intron 40.0%
!! TAGTGATGGATCTTGTGAAG+TGG - chr2.4:34824183-34824202 MS.gene045843:intron 40.0%
!!! ATTGGTTTTTGCAAGACACG+TGG + chr2.4:34825481-34825500 None:intergenic 40.0%
!!! GTCCAAGTGCTTGTTTTGAT+GGG + chr2.4:34826968-34826987 None:intergenic 40.0%
!!! GTTTTTTGAAGGACATGTGG+AGG - chr2.4:34824304-34824323 MS.gene045843:intron 40.0%
AAATAGCTGTGGCTGTAGTC+TGG + chr2.4:34826847-34826866 None:intergenic 45.0%
AAGAAATTTGCACGCTGGTC+TGG - chr2.4:34824383-34824402 MS.gene045843:intron 45.0%
ACATGGAGAGGATTGAAAGG+TGG + chr2.4:34825560-34825579 None:intergenic 45.0%
ACCGCTACTGGCAAAAAGAT+TGG + chr2.4:34826329-34826348 None:intergenic 45.0%
ACCTTATCCTCAGCGAGTAA+AGG + chr2.4:34823961-34823980 None:intergenic 45.0%
CCAAAGTTCACAAAGTGGGA+AGG + chr2.4:34826108-34826127 None:intergenic 45.0%
CCTCCCAAAGTTCACAAAGT+GGG + chr2.4:34826112-34826131 None:intergenic 45.0%
CCTTCCCACTTTGTGAACTT+TGG - chr2.4:34826105-34826124 MS.gene045843:intron 45.0%
CTGGTGGAACCAATATGGTT+TGG - chr2.4:34824337-34824356 MS.gene045843:intron 45.0%
CTGTCCTCTTGATGCTCTTT+GGG - chr2.4:34824467-34824486 MS.gene045843:intron 45.0%
CTTGAATCAAGTCTCTGCCA+TGG - chr2.4:34825337-34825356 MS.gene045843:intron 45.0%
GGTACATGGAGAGGATTGAA+AGG + chr2.4:34825563-34825582 None:intergenic 45.0%
GGTCTGTTAGTGGTAAACCA+TGG - chr2.4:34824421-34824440 MS.gene045843:intron 45.0%
GTAAGTTGTGCATCAGCCAA+AGG + chr2.4:34823628-34823647 None:intergenic 45.0%
GTAGGAAGAAATTTGCACGC+TGG - chr2.4:34824378-34824397 MS.gene045843:intron 45.0%
GTCACAGAACATGCAATCAC+TGG + chr2.4:34827011-34827030 None:intergenic 45.0%
GTCCCATCAAAACAAGCACT+TGG - chr2.4:34826963-34826982 MS.gene045843:intron 45.0%
TCATCTTCCTTTACTCGCTG+AGG - chr2.4:34823951-34823970 MS.gene045843:intron 45.0%
TCCTTTACTCGCTGAGGATA+AGG - chr2.4:34823957-34823976 MS.gene045843:intron 45.0%
TCTCTTGCCATTCCTTCTGT+TGG - chr2.4:34824282-34824301 MS.gene045843:intron 45.0%
TGATCACATGACTTCCCAAG+CGG - chr2.4:34826019-34826038 MS.gene045843:intron 45.0%
TGGCTCAGTTATTAAGGGAC+GGG + chr2.4:34826437-34826456 None:intergenic 45.0%
TGGTGGAGCTCTATACCTTT+GGG - chr2.4:34825725-34825744 MS.gene045843:intron 45.0%
TGTTACCGCCCAAACCATAT+TGG + chr2.4:34824349-34824368 None:intergenic 45.0%
TTGGTGGAGCTCTATACCTT+TGG - chr2.4:34825724-34825743 MS.gene045843:intron 45.0%
TTGTGGCTAGGATTACTCTG+CGG - chr2.4:34826693-34826712 MS.gene045843:intron 45.0%
! AGCTTTTCTCCTCTCAAACG+TGG - chr2.4:34825639-34825658 MS.gene045843:intron 45.0%
! CCACTTTGTGAACTTTGGGA+GGG - chr2.4:34826110-34826129 MS.gene045843:intron 45.0%
! CTCAGTTAATTTGGGACGGA+GGG - chr2.4:34826642-34826661 MS.gene045843:intron 45.0%
! TCAGCCAAAGGGTAAGCTTT+TGG + chr2.4:34823616-34823635 None:intergenic 45.0%
! TGATGGATCTTGTGAAGTGG+CGG - chr2.4:34824186-34824205 MS.gene045843:intron 45.0%
! TGGAACCAATATGGTTTGGG+CGG - chr2.4:34824341-34824360 MS.gene045843:intron 45.0%
! TTGGTAATAGGTCGGATCTC+AGG + chr2.4:34826784-34826803 None:intergenic 45.0%
! TTGTGAAGTGGCGGGAAAAA+GGG - chr2.4:34824195-34824214 MS.gene045843:intron 45.0%
!! AAAGAGCATCAAGAGGACAG+GGG + chr2.4:34824467-34824486 None:intergenic 45.0%
!! ACTGCATTTTGGTCTGGTAG+TGG + chr2.4:34825367-34825386 None:intergenic 45.0%
!! AGCTTGCTACTTCAACACTG+GGG + chr2.4:34825623-34825642 None:intergenic 45.0%
!! CAAAGAGCATCAAGAGGACA+GGG + chr2.4:34824468-34824487 None:intergenic 45.0%
!! CTCAAAAAGGGTGCCAATGA+AGG - chr2.4:34823694-34823713 MS.gene045843:CDS 45.0%
!! TTTTGAAGGACATGTGGAGG+CGG - chr2.4:34824307-34824326 MS.gene045843:intron 45.0%
!!! CGTCCAAGTGCTTGTTTTGA+TGG + chr2.4:34826969-34826988 None:intergenic 45.0%
!! AAAAATAAAAAATAAAACAA+AGG + chr2.4:34824104-34824123 None:intergenic 5.0%
!! AAATAATAAAAAAAAAACAA+AGG + chr2.4:34825693-34825712 None:intergenic 5.0%
!!! ATTTTTTATTTTTTTTTATG+GGG - chr2.4:34824111-34824130 MS.gene045843:intron 5.0%
!!! TATTATTTTAATTGAAAAAT+TGG - chr2.4:34825705-34825724 MS.gene045843:intron 5.0%
AAAGCGTGTTACCTCGACAC+TGG - chr2.4:34824155-34824174 MS.gene045843:intron 50.0%
AAGCGTGTTACCTCGACACT+GGG - chr2.4:34824156-34824175 MS.gene045843:intron 50.0%
ACACATGACTTCCCACTGTG+CGG - chr2.4:34825953-34825972 MS.gene045843:intron 50.0%
AGAACATGCAATCACTGGCC+GGG + chr2.4:34827006-34827025 None:intergenic 50.0%
ATGTGGAGGCGGTTAATCAC+TGG - chr2.4:34824318-34824337 MS.gene045843:intron 50.0%
CAGAACATGCAATCACTGGC+CGG + chr2.4:34827007-34827026 None:intergenic 50.0%
CAGGGGAGAGGATTGAAATG+TGG - chr2.4:34824220-34824239 MS.gene045843:intron 50.0%
CCCACTTTGTGAACTTTGGG+AGG - chr2.4:34826109-34826128 MS.gene045843:intron 50.0%
CCCTCCCAAAGTTCACAAAG+TGG + chr2.4:34826113-34826132 None:intergenic 50.0%
CCTGTCCTCTTGATGCTCTT+TGG - chr2.4:34824466-34824485 MS.gene045843:intron 50.0%
CGGTGGAACCAACATGGTTT+GGG + chr2.4:34825444-34825463 None:intergenic 50.0%
CTACTTCAACACTGGGGTGA+TGG + chr2.4:34825617-34825636 None:intergenic 50.0%
CTCAGTTATTAAGGGACGGG+GGG + chr2.4:34826434-34826453 None:intergenic 50.0%
GATGGATCTTGTGAAGTGGC+GGG - chr2.4:34824187-34824206 MS.gene045843:intron 50.0%
GCTCAGTTATTAAGGGACGG+GGG + chr2.4:34826435-34826454 None:intergenic 50.0%
GGAGAGGATTGAAAGGTGGA+TGG + chr2.4:34825556-34825575 None:intergenic 50.0%
GGCTCAGTTATTAAGGGACG+GGG + chr2.4:34826436-34826455 None:intergenic 50.0%
TCACTAAGCTTCACCGCTAC+TGG + chr2.4:34826341-34826360 None:intergenic 50.0%
TCTGCGGTCAACTGTAAGCT+GGG - chr2.4:34826709-34826728 MS.gene045843:intron 50.0%
TGCGGTTAGCGAACCACAAA+AGG - chr2.4:34825971-34825990 MS.gene045843:intron 50.0%
TGGGGCAATGTTTGAGAGGT+AGG - chr2.4:34824129-34824148 MS.gene045843:intron 50.0%
TTGGGCGGTGACAATGTCAA+GGG + chr2.4:34825426-34825445 None:intergenic 50.0%
TTTGGGCGGTAACAGTGTCA+CGG - chr2.4:34824355-34824374 MS.gene045843:intron 50.0%
TTTGGGCGGTGACAATGTCA+AGG + chr2.4:34825427-34825446 None:intergenic 50.0%
! CTTGTGAAGTGGCGGGAAAA+AGG - chr2.4:34824194-34824213 MS.gene045843:intron 50.0%
! GCTCAGTTAATTTGGGACGG+AGG - chr2.4:34826641-34826660 MS.gene045843:intron 50.0%
! GGTTTTTGCAAGACACGTGG+CGG + chr2.4:34825478-34825497 None:intergenic 50.0%
! TGGAACCAACATGGTTTGGG+CGG + chr2.4:34825441-34825460 None:intergenic 50.0%
! TGGTGGATCTGGTGAAGTAG+CGG + chr2.4:34825594-34825613 None:intergenic 50.0%
!! CCAAACCATGTTGGTTCCAC+CGG - chr2.4:34825442-34825461 MS.gene045843:intron 50.0%
!! CCAAAGAGCATCAAGAGGAC+AGG + chr2.4:34824469-34824488 None:intergenic 50.0%
!! GGTCTGGTAGTGGTAAACCA+TGG + chr2.4:34825357-34825376 None:intergenic 50.0%
AGCAGCAGCAGCAGCAACAA+GGG + chr2.4:34825665-34825684 None:intergenic 55.0%
AGCGTGTTACCTCGACACTG+GGG - chr2.4:34824157-34824176 MS.gene045843:intron 55.0%
CAACAAGGGCCACGTTTGAG+AGG + chr2.4:34825651-34825670 None:intergenic 55.0%
CAAGCACTTGGACGAGCATG+TGG - chr2.4:34826975-34826994 MS.gene045843:intron 55.0%
CCGGTGGAACCAACATGGTT+TGG + chr2.4:34825445-34825464 None:intergenic 55.0%
CTCTGCGGTCAACTGTAAGC+TGG - chr2.4:34826708-34826727 MS.gene045843:intron 55.0%
GAAGGAATGGCAAGAGACCC+AGG + chr2.4:34824279-34824298 None:intergenic 55.0%
GGGCGTGTCATGTGTGATTG+TGG - chr2.4:34824045-34824064 MS.gene045843:intron 55.0%
TCACTATCACCCCAGTGTCG+AGG + chr2.4:34824169-34824188 None:intergenic 55.0%
TGGAGGCGGTTAATCACTGG+TGG - chr2.4:34824321-34824340 MS.gene045843:intron 55.0%
TGGTTCGCTAACCGCACAGT+GGG + chr2.4:34825967-34825986 None:intergenic 55.0%
TGTCACCGCCCAAACCATGT+TGG - chr2.4:34825433-34825452 MS.gene045843:intron 55.0%
TTGGGCGGTAACAGTGTCAC+GGG - chr2.4:34824356-34824375 MS.gene045843:intron 55.0%
! AGTGGCGGGAAAAAGGGTAC+AGG - chr2.4:34824201-34824220 MS.gene045843:intron 55.0%
! CGACACTGGGGTGATAGTGA+TGG - chr2.4:34824169-34824188 MS.gene045843:intron 55.0%
! CTTCAACACTGGGGTGATGG+TGG + chr2.4:34825614-34825633 None:intergenic 55.0%
! GGAAAAAGGGTACAGGGGAG+AGG - chr2.4:34824208-34824227 MS.gene045843:intron 55.0%
! GTGGCGGGAAAAAGGGTACA+GGG - chr2.4:34824202-34824221 MS.gene045843:intron 55.0%
! TGGCGGGAAAAAGGGTACAG+GGG - chr2.4:34824203-34824222 MS.gene045843:intron 55.0%
!! GGTGCCCAAAGAGCATCAAG+AGG + chr2.4:34824474-34824493 None:intergenic 55.0%
CAGCAGCAGCAGCAGCAACA+AGG + chr2.4:34825666-34825685 None:intergenic 60.0%
GACGAGCATGTGGTGTGACC+CGG - chr2.4:34826985-34827004 MS.gene045843:CDS 60.0%
GCGGTAACAGTGTCACGGGT+AGG - chr2.4:34824360-34824379 MS.gene045843:intron 60.0%
GTGGTTCGCTAACCGCACAG+TGG + chr2.4:34825968-34825987 None:intergenic 60.0%
!! CAACACTGGGGTGATGGTGG+TGG + chr2.4:34825611-34825630 None:intergenic 60.0%
!! GAGCACCGGTGGAACCAACA+TGG + chr2.4:34825450-34825469 None:intergenic 60.0%
!! TGGGGTGATGGTGGTGGATC+TGG + chr2.4:34825605-34825624 None:intergenic 60.0%
ACGTGGCGGCGATTGAGCAC+CGG + chr2.4:34825464-34825483 None:intergenic 65.0%
!! TGGCGGCGATTGAGCACCGG+TGG + chr2.4:34825461-34825480 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 34823497 34827196 34823497 ID=MS.gene045843
chr2.4 mRNA 34823497 34827196 34823497 ID=MS.gene045843.t1;Parent=MS.gene045843
chr2.4 exon 34827194 34827196 34827194 ID=MS.gene045843.t1.exon1;Parent=MS.gene045843.t1
chr2.4 CDS 34827194 34827196 34827194 ID=cds.MS.gene045843.t1;Parent=MS.gene045843.t1
chr2.4 exon 34826978 34827098 34826978 ID=MS.gene045843.t1.exon2;Parent=MS.gene045843.t1
chr2.4 CDS 34826978 34827098 34826978 ID=cds.MS.gene045843.t1;Parent=MS.gene045843.t1
chr2.4 exon 34826715 34826818 34826715 ID=MS.gene045843.t1.exon3;Parent=MS.gene045843.t1
chr2.4 CDS 34826715 34826818 34826715 ID=cds.MS.gene045843.t1;Parent=MS.gene045843.t1
chr2.4 exon 34823617 34823748 34823617 ID=MS.gene045843.t1.exon4;Parent=MS.gene045843.t1
chr2.4 CDS 34823617 34823748 34823617 ID=cds.MS.gene045843.t1;Parent=MS.gene045843.t1
chr2.4 exon 34823497 34823523 34823497 ID=MS.gene045843.t1.exon5;Parent=MS.gene045843.t1
chr2.4 CDS 34823497 34823523 34823497 ID=cds.MS.gene045843.t1;Parent=MS.gene045843.t1
Gene Sequence

>MS.gene045843

ATGGTACCTTGTTTCCTTTCTCCTATTCATTTTTTTGAATTATTTTTATCGTTCCGTTTCTCAATTTTCTTCATTCAATCTTTTTTTTTTTGTTGCAGACAGGAGAAACAGTGAATCCAAAAGCTTACCCTTTGGCTGATGCACAACTTACGATAACGATAATGGATCTTGTTCAACAAGCTGCTAATTACAAGCAACTCAAAAAGGGTGCCAATGAAGGTGTGCTTTTGATTTATTTCTGTTTTTTGTTACGATTTTTATTTTCAGTTTCTATGGTTAAAAAGAAAAAGTGGTATTAGGGTTATGTTTGATTTATGACTAGTGCAACTGGATTGAATGGTTATGATTTTGTGTTGATTTATTTTATATGTTTTTCAGCTACAAAGACACTTAACAGAGGCATTTCTGAGTTTGTTGTGATGGCTGCAGACACTGAACCACTTGAGATTCTTCTTCATCTTCCTTTACTCGCTGAGGATAAGGTTAATTTCCAACAAATGTTAATTGCCTATTTTTTTATTTTTTTTAAATATGTCTGTGTTTTTGAAGGGCGTGTCATGTGTGATTGTGGTCATGTTTCCATTGTTACTGTTATTGCTGCTATCCTTTGTTTTATTTTTTATTTTTTTTTATGGGGCAATGTTTGAGAGGTAGGTGAAAAGCGTGTTACCTCGACACTGGGGTGATAGTGATGGATCTTGTGAAGTGGCGGGAAAAAGGGTACAGGGGAGAGGATTGAAATGTGGATAGAGATTTAGAAGGTAGAAAGTATTTATGAGCCTGGGTCTCTTGCCATTCCTTCTGTTGGTTTTTTGAAGGACATGTGGAGGCGGTTAATCACTGGTGGAACCAATATGGTTTGGGCGGTAACAGTGTCACGGGTAGGAAGAAATTTGCACGCTGGTCTGGTTAGATTATTGCGTTGGTCTGTTAGTGGTAAACCATGGTTGAGAATTGGTTTAGGAAACCCCTGTCCTCTTGATGCTCTTTGGGCACCCTTTGATTTGTTTAGACTTTAACGTAGACATAATCACTATGGAAAGACACTACTAGGCAATAAGTTCATGTTTATGCTAATTTTTTAAGTTCTGTACTTTTATTTAATTGAATTCTGATTATTTTGGTCAATTTTTGAACCACTTACATGTTTGATGAGACGTGATTATTAAAATGATTTATGTTTTGGCCAAATTTCTAAGCTCAGTACTCTCTTTTATCATTGAACTCTGATTATTTTGGTTGATTTTTTTTGTTCTACTTATATGTTGATGATATGCAATTAATAATCATATAGTAATATTTATTCATGACTTTTCAGTATTTAATTAATTTATTACAAGCCCTTTCTTGTGTCTGTTTCGTTTGTATTCTATTTAATTAGTTTGGTTTAATTCCTGTATGAAAAAATAGTTTGGATGAAGAACAAATTAATTCAAATCATATTCAATATTATACAAACAAAACAGACCAAAGGGCTTATATATGAACAAATACTAGAAAACTATGACGAAACATTACTATCTGATTAATAACATTACTATCTGATTAATAACATTACTATCTGATTAATAATCTCTCATCTTATCAACACATTAGTGGAACAAAAAACATTAACCAAAAAAATTAGAAATTCAATGAAATAATAGTAAAGAGCTTAGAAATTTGGCAGAAAAACGAGCCATTACAATCTGGTTAATAATCTAATATCATCAACACTAAAGTGGAACATAGAAAATTAAACAACAAAAATAATCATAATTCAAGGAAGTATGATTCATGTCTACGTCGATGTCCATACAAATCAAAAGGTTCCCAAAGAGCATCAAGATGACAAGGGTTTCTTGAATCAAGTCTCTGCCATGGTTTACCACTACCAGACCAAAATGCAGTAAACTAACACGAGCAGCACGCAAATGTCTACAACTACCCTTGACATTGTCACCGCCCAAACCATGTTGGTTCCACCGGTGCTCAATCGCCGCCACGTGTCTTGCAAAAACCAATAGATGTAGTGGCAGAGATCCAAACTCGTAAATCCTTTCTACCTTATGAATCTCCATCCACCTTTCAATCCTCTCCATGTACCCTTTTTTTCCGCTACTTCACCAGATCCACCACCATCACCCCAGTGTTGAAGTAGCAAGCTTTTCTCCTCTCAAACGTGGCCCTTGTTGCTGCTGCTGCTGCTGTTGTCCTTTGTTTTTTTTTTATTATTTTAATTGAAAAATTGGTGGAGCTCTATACCTTTGGGTTACTGTATAACTTTGGTTCTTTGGGTTACTGTCGATATCAATTTTATATTACAATGGGAAATGTTTCAAATTTTGATGCGCGTCTGACCCTGATGTTAATGCATTTGAGGGATTTAAAAATGAGGAATTAGTTATTTTTATATATATAGTTTAGATTCGTTTCTTGTGGAAAGTGGCTTGTTTTCCAACATGTTTGCAATCACGACACATGACTTCCCACTGTGCGGTTAGCGAACCACAAAAGGAAAAGGAAATCTTTGTTCAAACATGTGATCACATGACTTCCCAAGCGGTATGCATTAGAGTTTGATTTGCATTGATTACCTGCATGTATGCCATGTTGAAATTATGTAATGCCTTCCCACTTTGTGAACTTTGGGAGGGATTATTTAATGGAAATTGTTTTATTGGTAAGTTGGTAACGGTTAAAGTAATATGTGTAGTGGCTTTTTATCAAGTTTCTTTGCGGCTCCTTACCTTATCAAATTTCTTATCAAGTAATATGTGTAATGGCTTTTTATCAAGTTTCTTTCTGTGTTGAGCTTAAGTTGTATTTCACTATGAACAAGCCCCAAAACCAATCTTTTTGCCAGTAGCGGTGAAGCTTAGTGATACAGTGTCTAGTTTGATCCTTAAATTTTCAACGATCACACATTGCTCCGCATTGTCTTATACATATACTCCCCCCGTCCCTTAATAACTGAGCCATTTATCAAAAAAAAAGTGTCCCAAAATAACTTTTTCCCCTTTATAACTCTAATTAATACTACTTTCATAATTCCCAATTCAATATATTCCACTTTGCACCAAATACTTATCAAGAGCACTTAAAACCACTTTTCTCTCTCATTTATTTATACATTTTTCTTAATATGTGTGAAATGAGAAAATGGCTCAGTTAATTTGGGACGGAGGGAGTAATTGATTTCCATGTCTTTTTAAAATTGTGGCTAGGATTACTCTGCGGTCAACTGTAAGCTGGGTACCAAGACAAATCTAAAGTTTTTCAATAGCTATGTATGTTAATTTCGTCCTGAGATCCGACCTATTACCAATATATGCCAAATTGACTGATATTCCCCTGTTCTGTTTTTTCCAGACTACAGCCACAGCTATTTCTATTCATTCATGTTCTTGGCATTTTAATTTATGACAGTTGAAAAGGCTAATAACCATGTAATTATTTGAATTTTCAGAATGTTCCTTATGTATTTGTCCCATCAAAACAAGCACTTGGACGAGCATGTGGTGTGACCCGGCCAGTGATTGCATGTTCTGTGACAACCAATGAGGGAAGTCAACTTAAATCTCAAATACAACAACTAAAGGTATGCACCATGTTTTGCTTGATATTATTCATGTAGTCACCAACTTTATTAGCTGTTATAATGCCAACACATTTGGACATTCTTGATATGCAGGATGCTATTGAGAAGCTGTTGATCTGA
Protein sequence

>MS.gene045843.t1

MTGETVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADTEPLEILLHLPLLAEDKNVPYVFVPSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI