AlfalfaGEDB | MS.gene59047

M. sativa cultivar XinJiangDaYe / MS.gene59047

KEGG: K12845
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005730 GO:0008150 GO:0022613 GO:0032991 GO:0042254 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0071840 GO:0097159 GO:0110165 GO:1901363 GO:1990904
PFAM: PF01248.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59047.t1	XP_003609312.1	100	128	0	0	1	128	1	128	3.90E-62	247.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59047.t1	Q5XH16	82.0	128	23	0	1	128	1	128	1.3e-53	210.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59047.t1	B7FKG0	100.0	128	0	0	1	128	1	128	2.8e-62	247.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene59047	MS.gene035331	PPI
MS.gene59047	MS.gene053443	PPI
MS.gene59047	MS.gene030697	PPI
MS.gene59047	MS.gene70551	PPI
MS.gene59047	MS.gene62426	PPI
MS.gene59047	MS.gene047337	PPI
MS.gene59047	MS.gene037324	PPI
MS.gene59047	MS.gene61498	PPI
MS.gene59047	MS.gene054186	PPI
MS.gene59047	MS.gene58148	PPI
MS.gene59047	MS.gene045843	PPI
MS.gene59047	MS.gene056257	PPI
MS.gene59047	MS.gene01057	PPI
MS.gene59047	MS.gene036487	PPI
MS.gene59047	MS.gene59319	PPI
MS.gene59047	MS.gene70292	PPI
MS.gene59047	MS.gene022249	PPI
MS.gene59047	MS.gene033017	PPI
MS.gene59047	MS.gene50744	PPI
MS.gene59047	MS.gene072782	PPI
MS.gene59047	MS.gene073888	PPI
MS.gene59047	MS.gene010453	PPI
MS.gene59047	MS.gene033349	PPI
MS.gene59047	MS.gene047734	PPI
MS.gene59047	MS.gene51687	PPI
MS.gene074400	MS.gene59047	PPI
MS.gene59047	MS.gene019081	PPI
MS.gene59047	MS.gene99744	PPI
MS.gene59047	MS.gene003693	PPI
MS.gene59047	MS.gene025347	PPI
MS.gene59047	MS.gene065462	PPI
MS.gene59047	MS.gene029242	PPI
MS.gene011390	MS.gene59047	PPI
MS.gene036800	MS.gene59047	PPI
MS.gene59047	MS.gene038800	PPI
MS.gene59047	MS.gene85342	PPI
MS.gene59047	MS.gene95934	PPI
MS.gene59047	MS.gene038837	PPI
MS.gene59047	MS.gene002484	PPI
MS.gene59047	MS.gene036800	PPI
MS.gene59047	MS.gene25903	PPI
MS.gene59047	MS.gene001986	PPI
MS.gene59047	MS.gene63427	PPI
MS.gene59047	MS.gene062116	PPI
MS.gene59047	MS.gene059422	PPI
MS.gene59047	MS.gene01456	PPI
MS.gene59047	MS.gene23314	PPI
MS.gene033348	MS.gene59047	PPI
MS.gene59047	MS.gene057157	PPI
MS.gene59047	MS.gene009509	PPI
MS.gene59047	MS.gene92650	PPI
MS.gene59047	MS.gene074400	PPI
MS.gene59047	MS.gene28172	PPI
MS.gene59047	MS.gene041557	PPI
MS.gene59047	MS.gene41916	PPI
MS.gene59047	MS.gene070139	PPI
MS.gene59047	MS.gene51967	PPI
MS.gene59047	MS.gene065463	PPI
MS.gene59047	MS.gene68979	PPI
MS.gene59047	MS.gene70579	PPI
MS.gene59047	MS.gene015586	PPI
MS.gene59047	MS.gene019309	PPI
MS.gene59047	MS.gene53517	PPI
MS.gene59047	MS.gene044498	PPI
MS.gene59047	MS.gene067362	PPI
MS.gene59047	MS.gene51964	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59047.t1	MTR_4g114330	100.000	128	0	0	1	128	1	128	1.64e-91	260
MS.gene59047.t1	MTR_0011s0330	97.656	128	3	0	1	128	1	128	3.85e-89	254
MS.gene59047.t1	MTR_4g112780	34.483	116	75	1	4	118	17	132	1.45e-17	73.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59047.t1	AT4G22380	93.750	128	8	0	1	128	1	128	1.12e-76	223
MS.gene59047.t1	AT5G20160	92.969	128	9	0	1	128	1	128	4.77e-76	221
MS.gene59047.t1	AT4G12600	89.844	128	13	0	1	128	1	128	1.26e-72	213
MS.gene59047.t1	AT5G20160	74.375	160	9	1	1	128	1	160	3.79e-69	205
MS.gene59047.t1	AT4G12600	55.738	183	26	2	1	128	1	183	2.11e-49	156
MS.gene59047.t1	AT5G20160	65.741	108	5	1	1	76	1	108	1.11e-31	108
MS.gene59047.t1	AT5G08180	35.938	128	73	1	8	126	24	151	3.66e-21	83.6
MS.gene59047.t1	AT5G08180	35.938	128	73	1	8	126	24	151	3.66e-21	83.6

Find 28 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACTATAAGCAACTCAAAA+AGG	0.236397	8.4:-13825749	MS.gene59047:CDS
TATTTCTGAATTTGTTGTTA+TGG	0.302213	8.4:-13825606	MS.gene59047:CDS
TTGTTGAACAAGATCCATTA+TGG	0.342840	8.4:+13825777	None:intergenic
TCCGTTACTTGCTGAGGATA+AGG	0.383122	8.4:-13825546	MS.gene59047:intron
AGCTACTAAGACCTTGAATA+GGG	0.384258	8.4:-13825630	MS.gene59047:CDS
TCAGCCAAAGGGTATGCCTT+AGG	0.396497	8.4:+13825817	None:intergenic
CAGCTACTAAGACCTTGAAT+AGG	0.416543	8.4:-13825631	MS.gene59047:CDS
TTCAGAAATACCCCTATTCA+AGG	0.432400	8.4:+13825619	None:intergenic
TAACCCTAAGGCATACCCTT+TGG	0.467615	8.4:-13825821	MS.gene59047:CDS
TCAGCTAACAATCACCATAA+TGG	0.473714	8.4:-13825791	MS.gene59047:CDS
CAGCCAAAGGGTATGCCTTA+GGG	0.474338	8.4:+13825818	None:intergenic
CCTCAGCAAGTAACGGAAGA+TGG	0.475355	8.4:+13825552	None:intergenic
CCATCTTCCGTTACTTGCTG+AGG	0.512018	8.4:-13825552	MS.gene59047:CDS
GTCACATCAAAACAAGCACT+TGG	0.533789	8.4:-13824785	MS.gene59047:CDS
AGAACAAGCAATCACTGGTC+GGG	0.539786	8.4:+13824745	None:intergenic
TTTGATGTGACGAACACATA+GGG	0.556996	8.4:+13824797	None:intergenic
GTCACAGAACAAGCAATCAC+TGG	0.562045	8.4:+13824740	None:intergenic
AGGAGAAGCAGTTAACCCTA+AGG	0.573343	8.4:-13825833	MS.gene59047:CDS
CAAGCACTTGGGCGTGCTTG+TGG	0.582426	8.4:-13824773	MS.gene59047:CDS
TTAGCTGAGCATCAGCCAAA+GGG	0.590874	8.4:+13825806	None:intergenic
ACCTTATCCTCAGCAAGTAA+CGG	0.593594	8.4:+13825545	None:intergenic
GTTAGCTGAGCATCAGCCAA+AGG	0.598758	8.4:+13825805	None:intergenic
GTCTCAGATTCAGCAACTCA+AGG	0.599622	8.4:-13824693	MS.gene59047:intron
TGTTCTGTGACAACTAATGA+AGG	0.613805	8.4:-13824728	MS.gene59047:CDS
CAGAACAAGCAATCACTGGT+CGG	0.618383	8.4:+13824744	None:intergenic
AGATGGAGAAGAATCTCAAG+AGG	0.644746	8.4:+13825569	None:intergenic
GCTACTAAGACCTTGAATAG+GGG	0.659558	8.4:-13825629	MS.gene59047:CDS
TCACATCAAAACAAGCACTT+GGG	0.661984	8.4:-13824784	MS.gene59047:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAATGAAATAAGAAATT+AGG	+	chr8.4:13824808-13824827	None:intergenic	10.0%
!!	AAAAATGAAATAAGAAATTA+GGG	+	chr8.4:13824807-13824826	None:intergenic	10.0%
!!	AATATTGAAATAAAATGAAT+CGG	+	chr8.4:13825622-13825641	None:intergenic	10.0%
!!	AATTTACACAATTTATAATA+AGG	-	chr8.4:13825272-13825291	MS.gene59047:intron	10.0%
!!!	ATTTTATTTCAATATTTTTC+CGG	-	chr8.4:13825626-13825645	MS.gene59047:CDS	10.0%
!!	ATTAACAAGAAGATATAAAA+CGG	+	chr8.4:13824570-13824589	None:intergenic	15.0%
!!!	ATTTTAGTTTTTTCATAACT+TGG	-	chr8.4:13825089-13825108	MS.gene59047:intron	15.0%
!!	CAAATTAAGAGTTAAACATA+GGG	+	chr8.4:13824597-13824616	None:intergenic	20.0%
!!	GAAAATGAATTCTGAATATT+TGG	-	chr8.4:13825915-13825934	MS.gene59047:intron	20.0%
!!	GTGTAAATTAGTATTAGTAT+AGG	+	chr8.4:13825261-13825280	None:intergenic	20.0%
!!	TCAAATTAAGAGTTAAACAT+AGG	+	chr8.4:13824598-13824617	None:intergenic	20.0%
!!!	TATTTCTGAATTTGTTGTTA+TGG	-	chr8.4:13824908-13824927	MS.gene59047:intron	20.0%
!!!	TTTGTAAATGTATTTTGTCT+TGG	-	chr8.4:13825587-13825606	MS.gene59047:CDS	20.0%
!	AACTATAAGCAACTCAAAAA+GGG	-	chr8.4:13824766-13824785	MS.gene59047:CDS	25.0%
!	AGTAATATTACTTGAAGACA+TGG	+	chr8.4:13825524-13825543	None:intergenic	25.0%
!	ATAAAATGAATCGGATGAAA+TGG	+	chr8.4:13825613-13825632	None:intergenic	25.0%
!	ATGAATTCTGAATATTTGGT+TGG	-	chr8.4:13825919-13825938	MS.gene59047:intron	25.0%
!	CAAACCAAATCAATAATCAA+AGG	+	chr8.4:13824849-13824868	None:intergenic	25.0%
!	TAACTATAAGCAACTCAAAA+AGG	-	chr8.4:13824765-13824784	MS.gene59047:CDS	25.0%
!!	TTGTCCTTTGATTATTGATT+TGG	-	chr8.4:13824842-13824861	MS.gene59047:intron	25.0%
	ATGATGAAAAATTTGCAGAC+AGG	-	chr8.4:13824661-13824680	MS.gene59047:intron	30.0%
	ATGCTATGCATAACAAATAG+CGG	+	chr8.4:13825189-13825208	None:intergenic	30.0%
	TGTTATGCATAGCATCTTAT+TGG	-	chr8.4:13825194-13825213	MS.gene59047:intron	30.0%
	TTGTATCCATACAGATGTTT+GGG	-	chr8.4:13825226-13825245	MS.gene59047:intron	30.0%
	TTGTTGAACAAGATCCATTA+TGG	+	chr8.4:13824740-13824759	None:intergenic	30.0%
	TTTGTATCCATACAGATGTT+TGG	-	chr8.4:13825225-13825244	MS.gene59047:intron	30.0%
!!!	TGACTTTTAAAACAGGGTTA+TGG	-	chr8.4:13825321-13825340	MS.gene59047:intron	30.0%
	AGCTACTAAGACCTTGAATA+GGG	-	chr8.4:13824884-13824903	MS.gene59047:intron	35.0%
	CATGGTTTATCACACTTGTT+TGG	+	chr8.4:13825506-13825525	None:intergenic	35.0%
	CATTGCTGATAGACAAAAAC+CGG	+	chr8.4:13825648-13825667	None:intergenic	35.0%
	CTTGACATTTCAAAGCAACT+AGG	+	chr8.4:13825397-13825416	None:intergenic	35.0%
	GCTGAGGATAAGGTATATTA+GGG	-	chr8.4:13824978-13824997	MS.gene59047:intron	35.0%
	TCACATCAAAACAAGCACTT+GGG	-	chr8.4:13825730-13825749	MS.gene59047:intron	35.0%
	TCAGCTAACAATCACCATAA+TGG	-	chr8.4:13824723-13824742	MS.gene59047:CDS	35.0%
	TGCTATGCATAACAAATAGC+GGG	+	chr8.4:13825188-13825207	None:intergenic	35.0%
	TGCTGAGGATAAGGTATATT+AGG	-	chr8.4:13824977-13824996	MS.gene59047:intron	35.0%
	TGTTCTGTGACAACTAATGA+AGG	-	chr8.4:13825786-13825805	MS.gene59047:CDS	35.0%
	TTCAGAAATACCCCTATTCA+AGG	+	chr8.4:13824898-13824917	None:intergenic	35.0%
	TTTGATGTGACGAACACATA+GGG	+	chr8.4:13825720-13825739	None:intergenic	35.0%
!	TGAATATTTGGTTGGTATGC+AGG	-	chr8.4:13825927-13825946	MS.gene59047:intron	35.0%
!	TTTTGATGTGACGAACACAT+AGG	+	chr8.4:13825721-13825740	None:intergenic	35.0%
!!	CTAGTTGCTTTGAAATGTCA+AGG	-	chr8.4:13825395-13825414	MS.gene59047:intron	35.0%
!!!	GATCGCTGACTTTTAAAACA+GGG	-	chr8.4:13825315-13825334	MS.gene59047:intron	35.0%
!!!	TGATCGCTGACTTTTAAAAC+AGG	-	chr8.4:13825314-13825333	MS.gene59047:intron	35.0%
	ACAACATTCAGGGGAAAGAT+AGG	+	chr8.4:13825447-13825466	None:intergenic	40.0%
	ACCTTATCCTCAGCAAGTAA+CGG	+	chr8.4:13824972-13824991	None:intergenic	40.0%
	AGATGGAGAAGAATCTCAAG+AGG	+	chr8.4:13824948-13824967	None:intergenic	40.0%
	ATCGAGACCTAACAACATTC+AGG	+	chr8.4:13825458-13825477	None:intergenic	40.0%
	CAGCTACTAAGACCTTGAAT+AGG	-	chr8.4:13824883-13824902	MS.gene59047:intron	40.0%
	GCTACTAAGACCTTGAATAG+GGG	-	chr8.4:13824885-13824904	MS.gene59047:intron	40.0%
	GCTATGCATAACAAATAGCG+GGG	+	chr8.4:13825187-13825206	None:intergenic	40.0%
	GTCACATCAAAACAAGCACT+TGG	-	chr8.4:13825729-13825748	MS.gene59047:intron	40.0%
	TCGAGACCTAACAACATTCA+GGG	+	chr8.4:13825457-13825476	None:intergenic	40.0%
	TCTTTCCCCTGAATGTTGTT+AGG	-	chr8.4:13825448-13825467	MS.gene59047:intron	40.0%
!!	CTCAAAAAGGGTGCTAATGA+AGG	-	chr8.4:13824778-13824797	MS.gene59047:CDS	40.0%
	AGAACAAGCAATCACTGGTC+GGG	+	chr8.4:13825772-13825791	None:intergenic	45.0%
	AGGAGAAGCAGTTAACCCTA+AGG	-	chr8.4:13824681-13824700	MS.gene59047:intron	45.0%
	CAATGCAACACACGCAAGAA+AGG	+	chr8.4:13825564-13825583	None:intergenic	45.0%
	CAGAACAAGCAATCACTGGT+CGG	+	chr8.4:13825773-13825792	None:intergenic	45.0%
	CCACAACCCAAACATCTGTA+TGG	+	chr8.4:13825235-13825254	None:intergenic	45.0%
	CGAGACCTAACAACATTCAG+GGG	+	chr8.4:13825456-13825475	None:intergenic	45.0%
	GTCACAGAACAAGCAATCAC+TGG	+	chr8.4:13825777-13825796	None:intergenic	45.0%
	GTCTCAGATTCAGCAACTCA+AGG	-	chr8.4:13825821-13825840	MS.gene59047:CDS	45.0%
	TAACCCTAAGGCATACCCTT+TGG	-	chr8.4:13824693-13824712	MS.gene59047:intron	45.0%
	TTAGCTGAGCATCAGCCAAA+GGG	+	chr8.4:13824711-13824730	None:intergenic	45.0%
!	TCCGTTACTTGCTGAGGATA+AGG	-	chr8.4:13824968-13824987	MS.gene59047:intron	45.0%
!!	CCATACAGATGTTTGGGTTG+TGG	-	chr8.4:13825232-13825251	MS.gene59047:intron	45.0%
	GTTAGCTGAGCATCAGCCAA+AGG	+	chr8.4:13824712-13824731	None:intergenic	50.0%
!	CAGCCAAAGGGTATGCCTTA+GGG	+	chr8.4:13824699-13824718	None:intergenic	50.0%
!	CCATCTTCCGTTACTTGCTG+AGG	-	chr8.4:13824962-13824981	MS.gene59047:intron	50.0%
!	CCTCAGCAAGTAACGGAAGA+TGG	+	chr8.4:13824965-13824984	None:intergenic	50.0%
!	TCAGCCAAAGGGTATGCCTT+AGG	+	chr8.4:13824700-13824719	None:intergenic	50.0%
	CAAGCACTTGGGCGTGCTTG+TGG	-	chr8.4:13825741-13825760	MS.gene59047:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	13824561	13825975	13824561	ID=MS.gene59047
chr8.4	mRNA	13824561	13825975	13824561	ID=MS.gene59047.t1;Parent=MS.gene59047
chr8.4	exon	13825973	13825975	13825973	ID=MS.gene59047.t1.exon1;Parent=MS.gene59047.t1
chr8.4	CDS	13825973	13825975	13825973	ID=cds.MS.gene59047.t1;Parent=MS.gene59047.t1
chr8.4	exon	13825737	13825857	13825737	ID=MS.gene59047.t1.exon2;Parent=MS.gene59047.t1
chr8.4	CDS	13825737	13825857	13825737	ID=cds.MS.gene59047.t1;Parent=MS.gene59047.t1
chr8.4	exon	13825547	13825650	13825547	ID=MS.gene59047.t1.exon3;Parent=MS.gene59047.t1
chr8.4	CDS	13825547	13825650	13825547	ID=cds.MS.gene59047.t1;Parent=MS.gene59047.t1
chr8.4	exon	13824694	13824825	13824694	ID=MS.gene59047.t1.exon4;Parent=MS.gene59047.t1
chr8.4	CDS	13824694	13824825	13824694	ID=cds.MS.gene59047.t1;Parent=MS.gene59047.t1
chr8.4	exon	13824561	13824587	13824561	ID=MS.gene59047.t1.exon5;Parent=MS.gene59047.t1
chr8.4	CDS	13824561	13824587	13824561	ID=cds.MS.gene59047.t1;Parent=MS.gene59047.t1

Gene Sequence

>MS.gene59047

ATGGTACCGTTTTATATCTTCTTGTTAATTTTTCCCTATGTTTAACTCTTAATTTGATTCAGTGTTAATTGATTACTGATTAATACTTGTTTTTTTGTATATGATGAAAAATTTGCAGACAGGAGAAGCAGTTAACCCTAAGGCATACCCTTTGGCTGATGCTCAGCTAACAATCACCATAATGGATCTTGTTCAACAAGCTGCTAACTATAAGCAACTCAAAAAGGGTGCTAATGAAGGTAACCCTAATTTCTTATTTCATTTTTTGTTTAATGTCTTTTTTGTCCTTTGATTATTGATTTGGTTTGTTTTTGTTGTGTTGCAGCTACTAAGACCTTGAATAGGGGTATTTCTGAATTTGTTGTTATGGCTGCTGATGCTGAACCTCTTGAGATTCTTCTCCATCTTCCGTTACTTGCTGAGGATAAGGTATATTAGGGTTTTGTTTATGTAATGTATGATCTTCATATAATGAAATGAAATGACTAACTATGTTAACTAAATCTGTCACAAATTAAAAGTTAAACAATTTTAGTTTTTTCATAACTTGGAAATAAATGTTTTAGTTGTGAATATATATGATTATGCTATGCTTTTTCGTAGTTCACAAACAAAGAAATCGACCCCGCTATTTGTTATGCATAGCATCTTATTGGTACAATCATTTGTATCCATACAGATGTTTGGGTTGTGGTCTCCTATACTAATACTAATTTACACAATTTATAATAAGGCTGTAATTGCATTTGCTGCTGATCGCTGACTTTTAAAACAGGGTTATGGTATGTTTGTGTTATGATATGTTTATGTAGCTTATTGTTATTGTTATGTAGCCTAGTTGCTTTGAAATGTCAAGGTTGTGAAATTTAGTGCTCATTCACAGCCTATCTTTCCCCTGAATGTTGTTAGGTCTCGATGAGTTTGTTGTAACTTACTCATAAACCAAACAAGTGTGATAAACCATGTCTTCAAGTAATATTACTATGAACTGTCACAGTATCCTTTCTTGCGTGTGTTGCATTGCAATTTGTAAATGTATTTTGTCTTGGCCATTTCATCCGATTCATTTTATTTCAATATTTTTCCGGTTTTTGTCTATCAGCAATGTATAGATTATTAATCATGTGCACTATCTGTTTGAAATTTTTAGAATGTTCCCTATGTGTTCGTCACATCAAAACAAGCACTTGGGCGTGCTTGTGGTGTCACCCGACCAGTGATTGCTTGTTCTGTGACAACTAATGAAGGTAGTCAATTGAAGTCTCAGATTCAGCAACTCAAGGTATGCACTATGCTTCGTTTTCATGATTTTCATTTTCACTATTTATTGTAATTGTTATATGTTACTTTAATTGAAAATGAATTCTGAATATTTGGTTGGTATGCAGGATGCTATTGAGAAGCTGCTAATCTGA

Protein sequence

>MS.gene59047.t1

MTGEAVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVTSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI