AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067362

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067362.t1 MTR_0011s0330 100.000 128 0 0 1 128 1 128 9.70e-92 261
MS.gene067362.t1 MTR_4g114330 97.656 128 3 0 1 128 1 128 3.85e-89 254
MS.gene067362.t1 MTR_4g112780 34.821 112 72 1 8 118 21 132 1.18e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067362.t1 AT4G22380 93.750 128 8 0 1 128 1 128 2.75e-77 224
MS.gene067362.t1 AT5G20160 92.969 128 9 0 1 128 1 128 1.31e-76 223
MS.gene067362.t1 AT4G12600 91.406 128 11 0 1 128 1 128 2.62e-74 217
MS.gene067362.t1 AT5G20160 74.375 160 9 1 1 128 1 160 6.71e-70 207
MS.gene067362.t1 AT4G12600 56.284 183 25 2 1 128 1 183 1.76e-49 156
MS.gene067362.t1 AT5G20160 64.815 108 6 1 1 76 1 108 6.22e-31 107
MS.gene067362.t1 AT5G08180 36.641 131 74 1 5 126 21 151 3.54e-23 88.6
MS.gene067362.t1 AT5G08180 36.641 131 74 1 5 126 21 151 3.54e-23 88.6

Find 24 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGCCAAAGGGTAAGCTTT+TGG 0.274630 2.3:+36097258 None:intergenic
TAATTACAAGCAACTCAAAA+AGG 0.282256 2.3:-36097190 MS.gene067362:CDS
TCCTTTACTTGCTGAGGATA+AGG 0.346162 2.3:-36096901 MS.gene067362:intron
ACAACTTACGATAACGATAA+TGG 0.396702 2.3:-36097232 MS.gene067362:CDS
TTTAAGTTGACTTCCCTCAT+TGG 0.429117 2.3:+36093916 None:intergenic
CATTTCTGAGTTTGTTGTGA+TGG 0.445083 2.3:-36096961 MS.gene067362:CDS
TTTGATGGGACAAATACATA+AGG 0.470260 2.3:+36093998 None:intergenic
TCATCTTCCTTTACTTGCTG+AGG 0.472550 2.3:-36096907 MS.gene067362:CDS
GAATCCAAAAGCTTACCCTT+TGG 0.477883 2.3:-36097262 MS.gene067362:CDS
ACCTTATCCTCAGCAAGTAA+AGG 0.528330 2.3:+36096900 None:intergenic
GTCACAGAACATGCAATCAC+TGG 0.528661 2.3:+36093941 None:intergenic
ATCTCAAATACAGCAACTAA+AGG 0.530724 2.3:-36093894 MS.gene067362:intron
CAGAACATGCAATCACTGGC+CGG 0.535766 2.3:+36093945 None:intergenic
TAAGTTGTGCATCGGCCAAA+GGG 0.538128 2.3:+36097247 None:intergenic
GTAAGTTGTGCATCGGCCAA+AGG 0.547648 2.3:+36097246 None:intergenic
GTCCCATCAAAACAAGCACT+TGG 0.547834 2.3:-36093986 MS.gene067362:CDS
GACGAGCATGTGGTGTGACC+CGG 0.557034 2.3:-36093964 MS.gene067362:CDS
AGAACATGCAATCACTGGCC+GGG 0.572135 2.3:+36093946 None:intergenic
CGTTATCGTAAGTTGTGCAT+CGG 0.590171 2.3:+36097239 None:intergenic
CAAGCACTTGGACGAGCATG+TGG 0.613075 2.3:-36093974 MS.gene067362:CDS
TGTTCTGTGACAACCAATGA+GGG 0.643609 2.3:-36093929 MS.gene067362:CDS
AGATGAAGAAGAATCTCAAG+TGG 0.658274 2.3:+36096924 None:intergenic
ATGTTCTGTGACAACCAATG+AGG 0.691643 2.3:-36093930 MS.gene067362:CDS
GCTACGAAGACACTTAACAG+AGG 0.758236 2.3:-36096984 MS.gene067362:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATCAATTTTATATTACAA+TGG - chr2.3:36096032-36096051 MS.gene067362:intron 10.0%
!!! TATCAATTTTATATTACAAT+GGG - chr2.3:36096033-36096052 MS.gene067362:intron 10.0%
!!! TATTAAAATGATTTATGTTT+TGG - chr2.3:36094891-36094910 MS.gene067362:intron 10.0%
!! ACAAAAAAAAAAAAACACAA+AGG + chr2.3:36094362-36094381 None:intergenic 15.0%
!! ACAAAAATAATCATAATTCA+AGG - chr2.3:36095491-36095510 MS.gene067362:intron 15.0%
!! GTATTAATTAGAGTTATAAA+GGG + chr2.3:36096722-36096741 None:intergenic 15.0%
!! TATTAATTAGAGTTATAAAG+GGG + chr2.3:36096721-36096740 None:intergenic 15.0%
!! TGAAAATTCAAATAATTACA+TGG + chr2.3:36097103-36097122 None:intergenic 15.0%
!! TGTATTCTATTTAATTAGTT+TGG - chr2.3:36095091-36095110 MS.gene067362:intron 15.0%
!!! CATTGAATTTCTAATTTTTT+TGG + chr2.3:36095366-36095385 None:intergenic 15.0%
!!! GTGTTTTTTTTTTTTTGTAT+CGG - chr2.3:36094364-36094383 MS.gene067362:intron 15.0%
!!! TAATTGAATTCTGATTATTT+TGG - chr2.3:36094829-36094848 MS.gene067362:intron 15.0%
!!! TATTTTAATTGAAAAATTGG+TGG - chr2.3:36095957-36095976 MS.gene067362:intron 15.0%
!!! TGTTTTTTTTTTTTTGTATC+GGG - chr2.3:36094365-36094384 MS.gene067362:intron 15.0%
!!! TTTAATGGAAATTGTTTTAT+TGG - chr2.3:36096380-36096399 MS.gene067362:intron 15.0%
!! ATAAATAAATGAGAGAAAAG+TGG + chr2.3:36096810-36096829 None:intergenic 20.0%
!! ATTACAAATTTGATAAGGTA+AGG + chr2.3:36096466-36096485 None:intergenic 20.0%
!! CAAAGTGAATATATTGAATT+GGG + chr2.3:36096758-36096777 None:intergenic 20.0%
!! CAAATAATTACATGGTTATT+AGG + chr2.3:36097095-36097114 None:intergenic 20.0%
!! CAGAATTGAAAATAAATTAG+AGG + chr2.3:36095193-36095212 None:intergenic 20.0%
!! GGTATTAATTAGAGTTATAA+AGG + chr2.3:36096723-36096742 None:intergenic 20.0%
!! TAGTAAAGAGCTTAAAAATT+TGG - chr2.3:36095392-36095411 MS.gene067362:intron 20.0%
!! TTACCTTATCAAATTTGTAA+TGG - chr2.3:36096465-36096484 MS.gene067362:intron 20.0%
!!! AAATTGTTTTATTGGTAAGT+TGG - chr2.3:36096388-36096407 MS.gene067362:intron 20.0%
!!! AATATGTTTGTGTTTTTGAA+TGG - chr2.3:36094285-36094304 MS.gene067362:intron 20.0%
!!! ATATGTTTGTGTTTTTGAAT+GGG - chr2.3:36094286-36094305 MS.gene067362:intron 20.0%
!!! CTCTAATTTATTTTCAATTC+TGG - chr2.3:36095191-36095210 MS.gene067362:intron 20.0%
!!! GACACTTTTTTTTTGATAAA+TGG + chr2.3:36096683-36096702 None:intergenic 20.0%
! AAACGAAGAAAAATGAGAAA+CGG + chr2.3:36093812-36093831 None:intergenic 25.0%
! AAGCCATTACAAATTTGATA+AGG + chr2.3:36096471-36096490 None:intergenic 25.0%
! AATTACAAGCAACTCAAAAA+GGG - chr2.3:36093939-36093958 MS.gene067362:CDS 25.0%
! ATAAATGGCTCAGTTATTAA+GGG + chr2.3:36096668-36096687 None:intergenic 25.0%
! ATATGTGTGAAATGAGAAAA+TGG - chr2.3:36096842-36096861 MS.gene067362:intron 25.0%
! ATTTGAGGGATTTAAAAATG+AGG - chr2.3:36096100-36096119 MS.gene067362:intron 25.0%
! CAATGTCTAAACAAATCAAA+GGG + chr2.3:36094725-36094744 None:intergenic 25.0%
! GAATAATATCAAGCAAAACA+TGG + chr2.3:36097266-36097285 None:intergenic 25.0%
! GATTATTAACCAGATTGTAA+TGG + chr2.3:36095430-36095449 None:intergenic 25.0%
! GCAAAGTGAATATATTGAAT+TGG + chr2.3:36096759-36096778 None:intergenic 25.0%
! TAATATCATCAACACTAAAG+TGG - chr2.3:36095450-36095469 MS.gene067362:intron 25.0%
! TAATTACAAGCAACTCAAAA+AGG - chr2.3:36093938-36093957 MS.gene067362:CDS 25.0%
! TACATGGTTATTAGGTTATT+AGG + chr2.3:36097087-36097106 None:intergenic 25.0%
! TCAATGTCTAAACAAATCAA+AGG + chr2.3:36094726-36094745 None:intergenic 25.0%
! TCCTGTATGAAAAAATAGAT+TGG - chr2.3:36095120-36095139 MS.gene067362:intron 25.0%
! TTATAACAGCTAATAAAGGT+TGG + chr2.3:36097298-36097317 None:intergenic 25.0%
! TTCAGTTTCTATGATTAGAA+AGG - chr2.3:36094027-36094046 MS.gene067362:intron 25.0%
! TTTCTATTCATTCATGTTCT+TGG - chr2.3:36097035-36097054 MS.gene067362:intron 25.0%
!! ACATTGAACTCTGATTATTT+TGG - chr2.3:36094944-36094963 MS.gene067362:intron 25.0%
!! ATTTGGCATATATTGGTAAT+AGG + chr2.3:36096967-36096986 None:intergenic 25.0%
!! CTTTTTATCAAGTTTCTTTG+CGG - chr2.3:36096438-36096457 MS.gene067362:intron 25.0%
!! TAAAGGGGAAAAAGTTATTT+TGG + chr2.3:36096706-36096725 None:intergenic 25.0%
!!! AAAGGGGAAAAAGTTATTTT+GGG + chr2.3:36096705-36096724 None:intergenic 25.0%
!!! AACAAAAAGCTTGTTCTTTT+GGG - chr2.3:36095161-36095180 MS.gene067362:intron 25.0%
!!! TAACAAAAAGCTTGTTCTTT+TGG - chr2.3:36095160-36095179 MS.gene067362:intron 25.0%
!!! TCCAATCTATTTTTTCATAC+AGG + chr2.3:36095124-36095143 None:intergenic 25.0%
!!! TCCATGTCTTTTTAAAATTG+TGG - chr2.3:36096899-36096918 MS.gene067362:intron 25.0%
!!! TTTTATTGGTAAGTTGGTAA+CGG - chr2.3:36096394-36096413 MS.gene067362:intron 25.0%
!!! TTTTTTTACTTTTTGCAGAC+AGG - chr2.3:36093834-36093853 MS.gene067362:intron 25.0%
AAAGTTATACAGTAACCCAA+AGG + chr2.3:36095992-36096011 None:intergenic 30.0%
AAGTGCTCTTGATAAGTATT+TGG + chr2.3:36096783-36096802 None:intergenic 30.0%
ACAACTTACGATAACGATAA+TGG - chr2.3:36093896-36093915 MS.gene067362:CDS 30.0%
ACTATGGAAAGACACTATTA+GGG - chr2.3:36094759-36094778 MS.gene067362:intron 30.0%
AGAAAATGGCTCAGTTAATT+CGG - chr2.3:36096856-36096875 MS.gene067362:intron 30.0%
AGTATAGGTATAAGACAATG+CGG + chr2.3:36096637-36096656 None:intergenic 30.0%
AGTCTGGAAAAAACAAAACA+GGG + chr2.3:36097002-36097021 None:intergenic 30.0%
ATAACAGTAACAATGGAAAC+AGG + chr2.3:36094330-36094349 None:intergenic 30.0%
ATACAAACAAAACAGACCAA+AGG - chr2.3:36095240-36095259 MS.gene067362:intron 30.0%
ATCTCAAATACAGCAACTAA+AGG - chr2.3:36097234-36097253 MS.gene067362:CDS 30.0%
ATTAGAAAGGAAAAGTGGTA+GGG - chr2.3:36094040-36094059 MS.gene067362:intron 30.0%
CTATGATTAGAAAGGAAAAG+TGG - chr2.3:36094035-36094054 MS.gene067362:intron 30.0%
GATAAATGGCTCAGTTATTA+AGG + chr2.3:36096669-36096688 None:intergenic 30.0%
GGCATTACATAATTTCAACA+TGG + chr2.3:36096330-36096349 None:intergenic 30.0%
GGCATTATAACAGCTAATAA+AGG + chr2.3:36097302-36097321 None:intergenic 30.0%
GGTTAAAGTAATATGTGTAG+TGG - chr2.3:36096415-36096434 MS.gene067362:intron 30.0%
GTCTAGTTAGATTATTGCAT+TGG - chr2.3:36094630-36094649 MS.gene067362:intron 30.0%
GTGTGATCGTTGAAAATTTA+AGG + chr2.3:36096608-36096627 None:intergenic 30.0%
TACAAACAAAACAGACCAAA+GGG - chr2.3:36095241-36095260 MS.gene067362:intron 30.0%
TAGAAAGGATTTATGAGCTT+GGG - chr2.3:36094489-36094508 MS.gene067362:intron 30.0%
TAGTCTGGAAAAAACAAAAC+AGG + chr2.3:36097003-36097022 None:intergenic 30.0%
TAGTTTAGATTCGTTTCTTG+TGG - chr2.3:36096144-36096163 MS.gene067362:intron 30.0%
TCAGTCAATTTGGCATATAT+TGG + chr2.3:36096974-36096993 None:intergenic 30.0%
TCATCTTATCAACACATTAG+TGG - chr2.3:36095325-36095344 MS.gene067362:intron 30.0%
TGAATGAATAGAAATAGCTG+TGG + chr2.3:36097029-36097048 None:intergenic 30.0%
TGATTTATGATTAGTGCTAC+TGG - chr2.3:36094070-36094089 MS.gene067362:intron 30.0%
TGTTATAATGCCAACACATT+TGG - chr2.3:36097310-36097329 MS.gene067362:intron 30.0%
TTGTTCATATATAAGCCCTT+TGG + chr2.3:36095259-36095278 None:intergenic 30.0%
TTTGATGGGACAAATACATA+AGG + chr2.3:36097133-36097152 None:intergenic 30.0%
! TTTGGGTTACTGTATAACTT+TGG - chr2.3:36095991-36096010 MS.gene067362:intron 30.0%
!! ACTGTATAACTTTGGTTCTT+TGG - chr2.3:36095999-36096018 MS.gene067362:intron 30.0%
!! CTGTATAACTTTGGTTCTTT+GGG - chr2.3:36096000-36096019 MS.gene067362:intron 30.0%
!! GCCACAATTTTAAAAAGACA+TGG + chr2.3:36096903-36096922 None:intergenic 30.0%
!!! ACTAGCTAAAAGATTGGTTT+TGG + chr2.3:36096549-36096568 None:intergenic 30.0%
!!! CTAGCTAAAAGATTGGTTTT+GGG + chr2.3:36096548-36096567 None:intergenic 30.0%
!!! GTCTTTTTAAAATTGTGGCT+AGG - chr2.3:36096904-36096923 MS.gene067362:CDS 30.0%
!!! TAGCTAAAAGATTGGTTTTG+GGG + chr2.3:36096547-36096566 None:intergenic 30.0%
AAAAACGAGCCATTACAATC+TGG - chr2.3:36095418-36095437 MS.gene067362:intron 35.0%
AAACGAAACAGACACAAGAA+AGG + chr2.3:36095072-36095091 None:intergenic 35.0%
AACGAAACAGACACAAGAAA+GGG + chr2.3:36095071-36095090 None:intergenic 35.0%
AATGTGGATGGAGATTTAGA+AGG - chr2.3:36094466-36094485 MS.gene067362:intron 35.0%
AGATGAAGAAGAATCTCAAG+TGG + chr2.3:36094207-36094226 None:intergenic 35.0%
AGCAGCAATAACAGTAACAA+TGG + chr2.3:36094337-36094356 None:intergenic 35.0%
CACTATGGAAAGACACTATT+AGG - chr2.3:36094758-36094777 MS.gene067362:intron 35.0%
CATCAAAAACACACCTTCAT+TGG + chr2.3:36093967-36093986 None:intergenic 35.0%
CCTCAAATGCATTAACATCA+GGG + chr2.3:36096088-36096107 None:intergenic 35.0%
CCTGATGTTAATGCATTTGA+GGG - chr2.3:36096086-36096105 MS.gene067362:intron 35.0%
CGATGTCCATACAAATCAAA+AGG - chr2.3:36095535-36095554 MS.gene067362:intron 35.0%
GAAAATGGCTCAGTTAATTC+GGG - chr2.3:36096857-36096876 MS.gene067362:intron 35.0%
GATAAACCATGGTTGAGAAT+TGG - chr2.3:36094662-36094681 MS.gene067362:intron 35.0%
GATTAGAAAGGAAAAGTGGT+AGG - chr2.3:36094039-36094058 MS.gene067362:intron 35.0%
GCAAAAACCAATAGATGTAG+TGG - chr2.3:36095739-36095758 MS.gene067362:intron 35.0%
GTAGAAAGGATTTACGAGTT+TGG + chr2.3:36095772-36095791 None:intergenic 35.0%
GTAGAAAGGATTTATGAGCT+TGG - chr2.3:36094488-36094507 MS.gene067362:intron 35.0%
GTCTGGAAAAAACAAAACAG+GGG + chr2.3:36097001-36097020 None:intergenic 35.0%
TCAAGAATGTCCAAATGTGT+TGG + chr2.3:36097323-36097342 None:intergenic 35.0%
TGAATTGGGAATGATGAAAG+TGG + chr2.3:36096744-36096763 None:intergenic 35.0%
TGACGTAGACATAATCACTA+TGG - chr2.3:36094743-36094762 MS.gene067362:intron 35.0%
TGGAGATTCATAAGGTAGAA+AGG + chr2.3:36095786-36095805 None:intergenic 35.0%
TGGAGATTTAGAAGGTAGAA+AGG - chr2.3:36094474-36094493 MS.gene067362:intron 35.0%
TTGTCATCTTGATGCTCTTT+GGG + chr2.3:36095563-36095582 None:intergenic 35.0%
TTTGGACATTCTTGATATGC+AGG - chr2.3:36097328-36097347 MS.gene067362:intron 35.0%
! ATTAGTGCTACTGGATTGTA+TGG - chr2.3:36094079-36094098 MS.gene067362:intron 35.0%
! CTTTGGGAGGGATTATTTAA+TGG - chr2.3:36096365-36096384 MS.gene067362:intron 35.0%
! GGCATATATTGGTAATAGGT+CGG + chr2.3:36096963-36096982 None:intergenic 35.0%
! TGAACAAAGACTTCCTTTTG+TGG + chr2.3:36096236-36096255 None:intergenic 35.0%
!! AAAGAGCATCAAGATGACAA+GGG - chr2.3:36095563-36095582 MS.gene067362:intron 35.0%
!! ACCAATCTTTTAGCTAGTAG+CGG - chr2.3:36096551-36096570 MS.gene067362:intron 35.0%
!! CATTTCTGAGTTTGTTGTGA+TGG - chr2.3:36094167-36094186 MS.gene067362:intron 35.0%
!! GAGTACTGAGCTTAGAAATT+TGG + chr2.3:36094917-36094936 None:intergenic 35.0%
!! TTTAAGTTGACTTCCCTCAT+TGG + chr2.3:36097215-36097234 None:intergenic 35.0%
!!! TGGGAACCTTTTGATTTGTA+TGG + chr2.3:36095544-36095563 None:intergenic 35.0%
AAACTCTAATGCATACCGCT+TGG + chr2.3:36096280-36096299 None:intergenic 40.0%
AACTCTAATGCATACCGCTT+GGG + chr2.3:36096279-36096298 None:intergenic 40.0%
AAGGTGGATGGAGATTCATA+AGG + chr2.3:36095794-36095813 None:intergenic 40.0%
ACCGCTACTAGCTAAAAGAT+TGG + chr2.3:36096555-36096574 None:intergenic 40.0%
ACCTTATCCTCAGCAAGTAA+AGG + chr2.3:36094231-36094250 None:intergenic 40.0%
ATCTCTGCCACTACATCTAT+TGG + chr2.3:36095749-36095768 None:intergenic 40.0%
ATGGCTCAGTTATTAAGGGA+CGG + chr2.3:36096664-36096683 None:intergenic 40.0%
ATGTTCTGTGACAACCAATG+AGG - chr2.3:36097198-36097217 MS.gene067362:CDS 40.0%
CAGGGGAATATCAGTCAATT+TGG + chr2.3:36096984-36097003 None:intergenic 40.0%
CCCTCAAATGCATTAACATC+AGG + chr2.3:36096089-36096108 None:intergenic 40.0%
CCCTGATGTTAATGCATTTG+AGG - chr2.3:36096085-36096104 MS.gene067362:intron 40.0%
CCTAAACCAATTCTCAACCA+TGG + chr2.3:36094671-36094690 None:intergenic 40.0%
CGAAAAACCAACAGAAGGAA+TGG + chr2.3:36094522-36094541 None:intergenic 40.0%
CGTCTCATCAAACATGTAAG+TGG + chr2.3:36094867-36094886 None:intergenic 40.0%
CGTTATCGTAAGTTGTGCAT+CGG + chr2.3:36093892-36093911 None:intergenic 40.0%
CTGTCCTCTTAATGCTCTTT+GGG - chr2.3:36094697-36094716 MS.gene067362:intron 40.0%
CTTCCCACTTTGTGAACTTT+GGG - chr2.3:36096349-36096368 MS.gene067362:intron 40.0%
CTTGTCATCTTGATGCTCTT+TGG + chr2.3:36095564-36095583 None:intergenic 40.0%
GAATCCAAAAGCTTACCCTT+TGG - chr2.3:36093866-36093885 MS.gene067362:intron 40.0%
GAGAAACGGAACGAATGAAT+AGG + chr2.3:36093798-36093817 None:intergenic 40.0%
GATTCGTTTCTTGTGGAAAG+TGG - chr2.3:36096151-36096170 MS.gene067362:intron 40.0%
GCAATGTTTGAGAGGTGAAA+GGG - chr2.3:36094387-36094406 MS.gene067362:intron 40.0%
GGAACGAATGAATAGGAGAA+AGG + chr2.3:36093791-36093810 None:intergenic 40.0%
GGTATGCTAGTGATAAACCA+TGG - chr2.3:36094651-36094670 MS.gene067362:intron 40.0%
GTATGTAGAAATTTGCACGC+TGG - chr2.3:36094608-36094627 MS.gene067362:intron 40.0%
TCCTTCGAAAAACCAACAGA+AGG + chr2.3:36094527-36094546 None:intergenic 40.0%
TGTCGTGATTGCAAACATGT+TGG + chr2.3:36096185-36096204 None:intergenic 40.0%
TGTTACCGCCAAAACCATAT+TGG + chr2.3:36094579-36094598 None:intergenic 40.0%
TGTTCTGTGACAACCAATGA+GGG - chr2.3:36097199-36097218 MS.gene067362:CDS 40.0%
TTTCAACATGGCATACATGC+AGG + chr2.3:36096318-36096337 None:intergenic 40.0%
! AGGGTAAGCTTTTGGATTCA+CGG + chr2.3:36093865-36093884 None:intergenic 40.0%
! CCATGGTTGAGAATTGGTTT+AGG - chr2.3:36094668-36094687 MS.gene067362:intron 40.0%
! CTCGTGTTAGTTTACTGCAT+TGG + chr2.3:36095630-36095649 None:intergenic 40.0%
! GAAAAAAAGGGTACATGGAG+AGG + chr2.3:36095822-36095841 None:intergenic 40.0%
! GTTAGTTTACTGCATTGGTC+TGG + chr2.3:36095625-36095644 None:intergenic 40.0%
! TCATCTTCCTTTACTTGCTG+AGG - chr2.3:36094221-36094240 MS.gene067362:intron 40.0%
! TCCTTTACTTGCTGAGGATA+AGG - chr2.3:36094227-36094246 MS.gene067362:intron 40.0%
!! AAAGAGCATTAAGAGGACAG+GGG + chr2.3:36094697-36094716 None:intergenic 40.0%
!! AAAGCTTGCTACTTCAACAC+TGG + chr2.3:36095875-36095894 None:intergenic 40.0%
!! AAGCTTGCTACTTCAACACT+GGG + chr2.3:36095874-36095893 None:intergenic 40.0%
!! CAAAACCATATTGGTTCCAC+CGG + chr2.3:36094570-36094589 None:intergenic 40.0%
!! CAAAGAGCATCAAGATGACA+AGG - chr2.3:36095562-36095581 MS.gene067362:intron 40.0%
!! CAAAGAGCATTAAGAGGACA+GGG + chr2.3:36094698-36094717 None:intergenic 40.0%
!! TTGGTTTTTCGAAGGACATG+TGG - chr2.3:36094531-36094550 MS.gene067362:intron 40.0%
!!! ATTGGTTTTTGCAAGACACG+TGG + chr2.3:36095731-36095750 None:intergenic 40.0%
!!! GTCCAAGTGCTTGTTTTGAT+GGG + chr2.3:36097147-36097166 None:intergenic 40.0%
!!! TCCTTCTGTTGGTTTTTCGA+AGG - chr2.3:36094523-36094542 MS.gene067362:intron 40.0%
!!! TGGAACCAATATGGTTTTGG+CGG - chr2.3:36094571-36094590 MS.gene067362:intron 40.0%
AAATAGCTGTGGCTGTAGTC+TGG + chr2.3:36097018-36097037 None:intergenic 45.0%
ACATGGAGAGGATTGAAAGG+TGG + chr2.3:36095810-36095829 None:intergenic 45.0%
ATGGCTCAGTTAATTCGGGA+CGG - chr2.3:36096861-36096880 MS.gene067362:intron 45.0%
CCAAAGTTCACAAAGTGGGA+AGG + chr2.3:36096351-36096370 None:intergenic 45.0%
CCTCCCAAAGTTCACAAAGT+GGG + chr2.3:36096355-36096374 None:intergenic 45.0%
CCTGTCCTCTTAATGCTCTT+TGG - chr2.3:36094696-36094715 MS.gene067362:intron 45.0%
CCTTCCCACTTTGTGAACTT+TGG - chr2.3:36096348-36096367 MS.gene067362:intron 45.0%
CTCAGTTATTAAGGGACGGA+GGG + chr2.3:36096660-36096679 None:intergenic 45.0%
CTTGAATCAAGTCTCTGCCA+TGG - chr2.3:36095589-36095608 MS.gene067362:intron 45.0%
GCTACGAAGACACTTAACAG+AGG - chr2.3:36094144-36094163 MS.gene067362:intron 45.0%
GGAGAGGATTGAAATGTGGA+TGG - chr2.3:36094454-36094473 MS.gene067362:intron 45.0%
GGCAATGTTTGAGAGGTGAA+AGG - chr2.3:36094386-36094405 MS.gene067362:intron 45.0%
GGTACATGGAGAGGATTGAA+AGG + chr2.3:36095813-36095832 None:intergenic 45.0%
GTCACAGAACATGCAATCAC+TGG + chr2.3:36097190-36097209 None:intergenic 45.0%
GTCCCATCAAAACAAGCACT+TGG - chr2.3:36097142-36097161 MS.gene067362:intron 45.0%
TCTCTTGCCATTCCTTCTGT+TGG - chr2.3:36094512-36094531 MS.gene067362:intron 45.0%
TGATCACATGACTTCCCAAG+CGG - chr2.3:36096262-36096281 MS.gene067362:intron 45.0%
TGGTGGAGCTCTATACCTTT+GGG - chr2.3:36095974-36095993 MS.gene067362:intron 45.0%
TGTATCGGGCAATGTTTGAG+AGG - chr2.3:36094379-36094398 MS.gene067362:intron 45.0%
TGTGCAGTCAACTGTAAGCT+GGG - chr2.3:36096932-36096951 MS.gene067362:CDS 45.0%
TTGGTGGAGCTCTATACCTT+TGG - chr2.3:36095973-36095992 MS.gene067362:intron 45.0%
TTTGAGAGGTGAAAGGGTGA+CGG - chr2.3:36094393-36094412 MS.gene067362:intron 45.0%
! AGCTTTTCTCCTCTCAAACG+TGG - chr2.3:36095889-36095908 MS.gene067362:intron 45.0%
! CCACTTTGTGAACTTTGGGA+GGG - chr2.3:36096353-36096372 MS.gene067362:intron 45.0%
! CGGTGGAACCAATATGGTTT+TGG - chr2.3:36094568-36094587 MS.gene067362:intron 45.0%
! GGTGAAGTGGCGGAAAAAAA+GGG + chr2.3:36095834-36095853 None:intergenic 45.0%
! TAAGTTGTGCATCGGCCAAA+GGG + chr2.3:36093884-36093903 None:intergenic 45.0%
! TGGCGGAAAAAAAGGGTACA+TGG + chr2.3:36095827-36095846 None:intergenic 45.0%
! TGGTGAAGTGGCGGAAAAAA+AGG + chr2.3:36094444-36094425 None:intergenic 45.0%
! TGGTGAAGTGGCGGAAAAAA+GGG - chr2.3:36094425-36094444 MS.gene067362:intron 45.0%
! TTACTGCATTGGTCTGGTAG+TGG + chr2.3:36095619-36095638 None:intergenic 45.0%
! TTTTGGCGGTAACAGTGTCA+CGG - chr2.3:36094585-36094604 MS.gene067362:intron 45.0%
!! AGCTTGCTACTTCAACACTG+GGG + chr2.3:36095873-36095892 None:intergenic 45.0%
!! CCAAAGAGCATTAAGAGGAC+AGG + chr2.3:36094699-36094718 None:intergenic 45.0%
!! CTCAAAAAGGGTGCCAATGA+AGG - chr2.3:36093951-36093970 MS.gene067362:CDS 45.0%
!! GTTTTTCGAAGGACATGTGG+AGG - chr2.3:36094534-36094553 MS.gene067362:intron 45.0%
!!! CGTCCAAGTGCTTGTTTTGA+TGG + chr2.3:36097148-36097167 None:intergenic 45.0%
!! AAAATAATAAAAAAAAACAA+AGG + chr2.3:36095943-36095962 None:intergenic 5.0%
!!! TATTATTTTAATTGAAAAAT+TGG - chr2.3:36095954-36095973 MS.gene067362:intron 5.0%
ACACATGACTTCCCACTGTG+CGG - chr2.3:36096202-36096221 MS.gene067362:intron 50.0%
AGAACATGCAATCACTGGCC+GGG + chr2.3:36097185-36097204 None:intergenic 50.0%
AGCAGCAGCAGCAACAACAA+GGG + chr2.3:36095915-36095934 None:intergenic 50.0%
CAGAACATGCAATCACTGGC+CGG + chr2.3:36097186-36097205 None:intergenic 50.0%
CAGGGGAGAGGATTGAAATG+TGG - chr2.3:36094450-36094469 MS.gene067362:intron 50.0%
CCCACTTTGTGAACTTTGGG+AGG - chr2.3:36096352-36096371 MS.gene067362:intron 50.0%
CCCTCCCAAAGTTCACAAAG+TGG + chr2.3:36096356-36096375 None:intergenic 50.0%
CGGTGGAACCAACATGGTTT+GGG + chr2.3:36095694-36095713 None:intergenic 50.0%
CTACTTCAACACTGGGGTGA+TGG + chr2.3:36095867-36095886 None:intergenic 50.0%
CTCAGTTAATTCGGGACGGA+GGG - chr2.3:36096865-36096884 MS.gene067362:intron 50.0%
CTGTGCAGTCAACTGTAAGC+TGG - chr2.3:36096931-36096950 MS.gene067362:CDS 50.0%
GCTCAGTTATTAAGGGACGG+AGG + chr2.3:36096661-36096680 None:intergenic 50.0%
GGAGAGGATTGAAAGGTGGA+TGG + chr2.3:36095806-36095825 None:intergenic 50.0%
TGCGGTTAGCGAACCACAAA+AGG - chr2.3:36096220-36096239 MS.gene067362:intron 50.0%
TTGGGCGGTGACAATGTCAA+GGG + chr2.3:36095676-36095695 None:intergenic 50.0%
TTTCGAAGGACATGTGGAGG+CGG - chr2.3:36094537-36094556 MS.gene067362:intron 50.0%
TTTGGCGGTAACAGTGTCAC+GGG - chr2.3:36094586-36094605 MS.gene067362:intron 50.0%
TTTGGGCGGTGACAATGTCA+AGG + chr2.3:36095677-36095696 None:intergenic 50.0%
! AGTGGCGGAAAAAAGGGTAC+AGG - chr2.3:36094431-36094450 MS.gene067362:intron 50.0%
! CTGGTGAAGTGGCGGAAAAA+AGG - chr2.3:36094424-36094443 MS.gene067362:intron 50.0%
! GAAAAAAGGGTACAGGGGAG+AGG - chr2.3:36094438-36094457 MS.gene067362:intron 50.0%
! GGTTTTTGCAAGACACGTGG+CGG + chr2.3:36095728-36095747 None:intergenic 50.0%
! GTAAGTTGTGCATCGGCCAA+AGG + chr2.3:36093885-36093904 None:intergenic 50.0%
! GTGGCGGAAAAAAGGGTACA+GGG - chr2.3:36094432-36094451 MS.gene067362:intron 50.0%
! TCGGCCAAAGGGTAAGCTTT+TGG + chr2.3:36093873-36093892 None:intergenic 50.0%
! TGATGGATCTGGTGAAGTGG+CGG - chr2.3:36094416-36094435 MS.gene067362:intron 50.0%
! TGGAACCAACATGGTTTGGG+CGG + chr2.3:36095691-36095710 None:intergenic 50.0%
! TGGCGGAAAAAAGGGTACAG+GGG - chr2.3:36094433-36094452 MS.gene067362:intron 50.0%
! TTAAGGGACGGAGGGAGTAT+AGG + chr2.3:36096652-36096671 None:intergenic 50.0%
!! AAGCACCGGTGGAACCAATA+TGG - chr2.3:36094562-36094581 MS.gene067362:intron 50.0%
!! CCAAACCATGTTGGTTCCAC+CGG - chr2.3:36095692-36095711 MS.gene067362:intron 50.0%
!! GGTCTGGTAGTGGTAAACCA+TGG + chr2.3:36095609-36095628 None:intergenic 50.0%
!! GGTGCCCAAAGAGCATTAAG+AGG + chr2.3:36094704-36094723 None:intergenic 50.0%
ATGTGGAGGCGGTTAAGCAC+CGG - chr2.3:36094548-36094567 MS.gene067362:intron 55.0%
CAACAAGGGCCACGTTTGAG+AGG + chr2.3:36095901-36095920 None:intergenic 55.0%
CAAGCACTTGGACGAGCATG+TGG - chr2.3:36097154-36097173 MS.gene067362:intron 55.0%
CAGCAGCAGCAGCAACAACA+AGG + chr2.3:36095916-36095935 None:intergenic 55.0%
CCGGTGGAACCAACATGGTT+TGG + chr2.3:36095695-36095714 None:intergenic 55.0%
GCTCAGTTAATTCGGGACGG+AGG - chr2.3:36096864-36096883 MS.gene067362:intron 55.0%
TGGTTCGCTAACCGCACAGT+GGG + chr2.3:36096216-36096235 None:intergenic 55.0%
TGTCACCGCCCAAACCATGT+TGG - chr2.3:36095683-36095702 MS.gene067362:intron 55.0%
! AGGTGAAAGGGTGACGGTGA+TGG - chr2.3:36094399-36094418 MS.gene067362:intron 55.0%
! CTTCAACACTGGGGTGATGG+TGG + chr2.3:36095864-36095883 None:intergenic 55.0%
! TGGTGGATCTGGTGAAGTGG+CGG + chr2.3:36095844-36095863 None:intergenic 55.0%
!! AAGGGTGACGGTGATGGATC+TGG - chr2.3:36094405-36094424 MS.gene067362:intron 55.0%
!! CGGTGATGGATCTGGTGAAG+TGG - chr2.3:36094413-36094432 MS.gene067362:intron 55.0%
!! TGGTGGTGGATCTGGTGAAG+TGG + chr2.3:36095847-36095866 None:intergenic 55.0%
GACGAGCATGTGGTGTGACC+CGG - chr2.3:36097164-36097183 MS.gene067362:intron 60.0%
GTGGTTCGCTAACCGCACAG+TGG + chr2.3:36096217-36096236 None:intergenic 60.0%
!! CAACACTGGGGTGATGGTGG+TGG + chr2.3:36095861-36095880 None:intergenic 60.0%
!! GAGCACCGGTGGAACCAACA+TGG + chr2.3:36095700-36095719 None:intergenic 60.0%
!! TGGGGTGATGGTGGTGGATC+TGG + chr2.3:36095855-36095874 None:intergenic 60.0%
ACGTGGCGGCGATTGAGCAC+CGG + chr2.3:36095714-36095733 None:intergenic 65.0%
!! TGGAGGCGGTTAAGCACCGG+TGG - chr2.3:36094551-36094570 MS.gene067362:intron 65.0%
!! TGGCGGCGATTGAGCACCGG+TGG + chr2.3:36095711-36095730 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 36093774 36097376 36093774 ID=MS.gene067362
chr2.3 mRNA 36093774 36097376 36093774 ID=MS.gene067362.t1;Parent=MS.gene067362
chr2.3 exon 36097374 36097376 36097374 ID=MS.gene067362.t1.exon1;Parent=MS.gene067362.t1
chr2.3 CDS 36097374 36097376 36097374 ID=cds.MS.gene067362.t1;Parent=MS.gene067362.t1
chr2.3 exon 36097178 36097298 36097178 ID=MS.gene067362.t1.exon2;Parent=MS.gene067362.t1
chr2.3 CDS 36097178 36097298 36097178 ID=cds.MS.gene067362.t1;Parent=MS.gene067362.t1
chr2.3 exon 36096902 36097005 36096902 ID=MS.gene067362.t1.exon3;Parent=MS.gene067362.t1
chr2.3 CDS 36096902 36097005 36096902 ID=cds.MS.gene067362.t1;Parent=MS.gene067362.t1
chr2.3 exon 36093895 36094026 36093895 ID=MS.gene067362.t1.exon4;Parent=MS.gene067362.t1
chr2.3 CDS 36093895 36094026 36093895 ID=cds.MS.gene067362.t1;Parent=MS.gene067362.t1
chr2.3 exon 36093774 36093800 36093774 ID=MS.gene067362.t1.exon5;Parent=MS.gene067362.t1
chr2.3 CDS 36093774 36093800 36093774 ID=cds.MS.gene067362.t1;Parent=MS.gene067362.t1
Gene Sequence

>MS.gene067362

ATGGTAACTCGTTTCCTTTCTCCTATTCATTCGTTCCGTTTCTCATTTTTCTTCGTTTAATTTTTTTACTTTTTGCAGACAGGAGAAACCGTGAATCCAAAAGCTTACCCTTTGGCCGATGCACAACTTACGATAACGATAATGGATCTTGTTCAACAAGCTGCTAATTACAAGCAACTCAAAAAGGGTGCCAATGAAGGTGTGTTTTTGATGAATATTCGTTCATTTCTGCTTTTGTGTTATGAGTTTTATCTTCAGTTTCTATGATTAGAAAGGAAAAGTGGTAGGGTTATGTTTGATTTATGATTAGTGCTACTGGATTGTATGGTTTTGATTTTGTGTTGATTTATTTTATATGTTTTTCATTTCAGCTACGAAGACACTTAACAGAGGCATTTCTGAGTTTGTTGTGATGGCTGCAGACACTGAACCACTTGAGATTCTTCTTCATCTTCCTTTACTTGCTGAGGATAAGGTTAATTTTCAACAAATGTTATTTTTTTATTTTTTTAATATGTTTGTGTTTTTGAATGGGCGTGTCATGTGTGATTGTCCTGTTTCCATTGTTACTGTTATTGCTGCTGTCCTTTGTGTTTTTTTTTTTTTGTATCGGGCAATGTTTGAGAGGTGAAAGGGTGACGGTGATGGATCTGGTGAAGTGGCGGAAAAAAGGGTACAGGGGAGAGGATTGAAATGTGGATGGAGATTTAGAAGGTAGAAAGGATTTATGAGCTTGGGTCTCTTGCCATTCCTTCTGTTGGTTTTTCGAAGGACATGTGGAGGCGGTTAAGCACCGGTGGAACCAATATGGTTTTGGCGGTAACAGTGTCACGGGTATGTAGAAATTTGCACGCTGGTCTAGTTAGATTATTGCATTGGTATGCTAGTGATAAACCATGGTTGAGAATTGGTTTAGGAAACCCCTGTCCTCTTAATGCTCTTTGGGCACCCTTTGATTTGTTTAGACATTGACGTAGACATAATCACTATGGAAAGACACTATTAGGGCAATAAGTTCATGTTTATGCTAATTTTTTAAGTTCTGTACTTTTATTTAATTGAATTCTGATTATTTTGGTCAATTTTTGTACCACTTACATGTTTGATGAGACGTGATTATTAAAATGATTTATGTTTTGGCCAAATTTCTAAGCTCAGTACTCTCTTTTAACATTGAACTCTGATTATTTTGGTTGATTTTTTTTTTGTTCTACTATGTGTTGAAGATATGCAATTAATAACATATAGTAATATTTATTCATGACTTGTCAGTATTTAATTAATTTATTACAAGCCCTTTCTTGTGTCTGTTTCGTTTGTATTCTATTTAATTAGTTTGGTTTAATTCCTGTATGAAAAAATAGATTGGATGAAGAACAAAAAGATTAACAAAAAGCTTGTTCTTTTGGGTTACTTCCTCTAATTTATTTTCAATTCTGGAATTAATTCAAATCATATTCAATATTATACAAACAAAACAGACCAAAGGGCTTATATATGAACAAATACTAGAAAACTATGACGAAACATTACTATCTGATTAATAATCTCTCATCTTATCAACACATTAGTGGAACAAAAAACATTAACCAAAAAAATTAGAAATTCAATGAAATAATAGTAAAGAGCTTAAAAATTTGGCAGAAAAACGAGCCATTACAATCTGGTTAATAATCTAATATCATCAACACTAAAGTGGAACATAGAAAATTAAACAACAAAAATAATCATAATTCAAGGAAGTATGATTCATGTCTACGTCGATGTCCATACAAATCAAAAGGTTCCCAAAGAGCATCAAGATGACAAGGGTTTCTTGAATCAAGTCTCTGCCATGGTTTACCACTACCAGACCAATGCAGTAAACTAACACGAGCAGCACGCAAATGTCTACAACTACCCTTGACATTGTCACCGCCCAAACCATGTTGGTTCCACCGGTGCTCAATCGCCGCCACGTGTCTTGCAAAAACCAATAGATGTAGTGGCAGAGATCCAAACTCGTAAATCCTTTCTACCTTATGAATCTCCATCCACCTTTCAATCCTCTCCATGTACCCTTTTTTTCCGCCACTTCACCAGATCCACCACCATCACCCCAGTGTTGAAGTAGCAAGCTTTTCTCCTCTCAAACGTGGCCCTTGTTGTTGCTGCTGCTGCTGTTGTCCTTTGTTTTTTTTTATTATTTTAATTGAAAAATTGGTGGAGCTCTATACCTTTGGGTTACTGTATAACTTTGGTTCTTTGGGTTACTGTCGATATCAATTTTATATTACAATGGGAAATGTTTCAAATTTTGATGCGCGTCTGACCCTGATGTTAATGCATTTGAGGGATTTAAAAATGAGGAATTAGTTATTTGTATATATATAGTTTAGATTCGTTTCTTGTGGAAAGTGGCTTGTTTTCCAACATGTTTGCAATCACGACACATGACTTCCCACTGTGCGGTTAGCGAACCACAAAAGGAAGTCTTTGTTCAAACATGTGATCACATGACTTCCCAAGCGGTATGCATTAGAGTTTGATTTGCATTGATTACCTGCATGTATGCCATGTTGAAATTATGTAATGCCTTCCCACTTTGTGAACTTTGGGAGGGATTATTTAATGGAAATTGTTTTATTGGTAAGTTGGTAACGGTTAAAGTAATATGTGTAGTGGCTTTTTATCAAGTTTCTTTGCGGCTCCTTACCTTATCAAATTTGTAATGGCTTTTTATCAAGTTTCTTTCTGTGTTGAGCTTAAGTTGTATTTCACTATGAACAAGCCCCAAAACCAATCTTTTAGCTAGTAGCGGTGAAGCTTAGTGATACAGTGTCTAGTTTGATCCTTAAATTTTCAACGATCACACATTGCACCGCATTGTCTTATACCTATACTCCCTCCGTCCCTTAATAACTGAGCCATTTATCAAAAAAAAAGTGTCCCAAAATAACTTTTTCCCCTTTATAACTCTAATTAATACCACTTTCATCATTCCCAATTCAATATATTCACTTTGCACCAAATACTTATCAAGAGCACTTAAAACCACTTTTCTCTCATTTATTTATACATTTTTCTTAATATGTGTGAAATGAGAAAATGGCTCAGTTAATTCGGGACGGAGGGAGTAATTAATTTCCATGTCTTTTTAAAATTGTGGCTAGGATTACTGTGCAGTCAACTGTAAGCTGGGTACATCCGACCTATTACCAATATATGCCAAATTGACTGATATTCCCCTGTTTTGTTTTTTCCAGACTACAGCCACAGCTATTTCTATTCATTCATGTTCTTGGCATTTTAATTTATGACAGTTGAAAAGCCTAATAACCTAATAACCATGTAATTATTTGAATTTTCAGAATGTTCCTTATGTATTTGTCCCATCAAAACAAGCACTTGGACGAGCATGTGGTGTGACCCGGCCAGTGATTGCATGTTCTGTGACAACCAATGAGGGAAGTCAACTTAAATCTCAAATACAGCAACTAAAGGTATGCACCATGTTTTGCTTGATATTATTCATGTAGTCACCAACCTTTATTAGCTGTTATAATGCCAACACATTTGGACATTCTTGATATGCAGGATGCTATTGAGAAGCTGTTGATCTGA
Protein sequence

>MS.gene067362.t1

MTGETVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADTEPLEILLHLPLLAEDKNVPYVFVPSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI