AlfalfaGEDB | MS.gene62426

M. sativa cultivar XinJiangDaYe / MS.gene62426

KEGG: K12845
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005730 GO:0008150 GO:0022613 GO:0032991 GO:0042254 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0071840 GO:0097159 GO:0110165 GO:1901363 GO:1990904
PFAM: PF01248.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62426.t1	XP_003609312.1	100	128	0	0	1	128	1	128	3.90E-62	247.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62426.t1	Q5XH16	82.0	128	23	0	1	128	1	128	1.3e-53	210.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62426.t1	B7FKG0	100.0	128	0	0	1	128	1	128	2.8e-62	247.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049306	MS.gene62426	0.804095	2.55E-49	-1.69E-46
MS.gene054340	MS.gene62426	0.815401	9.42E-52	-1.69E-46
MS.gene057321	MS.gene62426	0.808549	2.93E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene011390	MS.gene62426	PPI
MS.gene059422	MS.gene62426	PPI
MS.gene036800	MS.gene62426	PPI
MS.gene036487	MS.gene62426	PPI
MS.gene59047	MS.gene62426	PPI
MS.gene62426	MS.gene019081	PPI
MS.gene59319	MS.gene62426	PPI
MS.gene62426	MS.gene99744	PPI
MS.gene62426	MS.gene025347	PPI
MS.gene003693	MS.gene62426	PPI
MS.gene62426	MS.gene065462	PPI
MS.gene62426	MS.gene029242	PPI
MS.gene033349	MS.gene62426	PPI
MS.gene62426	MS.gene038800	PPI
MS.gene033348	MS.gene62426	PPI
MS.gene62426	MS.gene85342	PPI
MS.gene63427	MS.gene62426	PPI
MS.gene037324	MS.gene62426	PPI
MS.gene002484	MS.gene62426	PPI
MS.gene62426	MS.gene25903	PPI
MS.gene62426	MS.gene001986	PPI
MS.gene62426	MS.gene062116	PPI
MS.gene038837	MS.gene62426	PPI
MS.gene62426	MS.gene23314	PPI
MS.gene62426	MS.gene057157	PPI
MS.gene62426	MS.gene28172	PPI
MS.gene62426	MS.gene041557	PPI
MS.gene62426	MS.gene41916	PPI
MS.gene62426	MS.gene070139	PPI
MS.gene62426	MS.gene065463	PPI
MS.gene61498	MS.gene62426	PPI
MS.gene01456	MS.gene62426	PPI
MS.gene68979	MS.gene62426	PPI
MS.gene62426	MS.gene70579	PPI
MS.gene62426	MS.gene015586	PPI
MS.gene01057	MS.gene62426	PPI
MS.gene58148	MS.gene62426	PPI
MS.gene045843	MS.gene62426	PPI
MS.gene95934	MS.gene62426	PPI
MS.gene53517	MS.gene62426	PPI
MS.gene62426	MS.gene019309	PPI
MS.gene51687	MS.gene62426	PPI
MS.gene62426	MS.gene044498	PPI
MS.gene62426	MS.gene067362	PPI
MS.gene62426	MS.gene51964	PPI
MS.gene62426	MS.gene035331	PPI
MS.gene70551	MS.gene62426	PPI
MS.gene009509	MS.gene62426	PPI
MS.gene62426	MS.gene053443	PPI
MS.gene62426	MS.gene030697	PPI
MS.gene51967	MS.gene62426	PPI
MS.gene62426	MS.gene047337	PPI
MS.gene62426	MS.gene054186	PPI
MS.gene62426	MS.gene056257	PPI
MS.gene62426	MS.gene01057	PPI
MS.gene047734	MS.gene62426	PPI
MS.gene074400	MS.gene62426	PPI
MS.gene62426	MS.gene70292	PPI
MS.gene62426	MS.gene022249	PPI
MS.gene62426	MS.gene033017	PPI
MS.gene62426	MS.gene50744	PPI
MS.gene62426	MS.gene072782	PPI
MS.gene92650	MS.gene62426	PPI
MS.gene62426	MS.gene073888	PPI
MS.gene62426	MS.gene010453	PPI
MS.gene067362	MS.gene62426	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62426.t1	MTR_4g114330	100.000	128	0	0	1	128	1	128	1.64e-91	260
MS.gene62426.t1	MTR_0011s0330	97.656	128	3	0	1	128	1	128	3.85e-89	254
MS.gene62426.t1	MTR_4g112780	34.483	116	75	1	4	118	17	132	1.45e-17	73.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62426.t1	AT4G22380	93.750	128	8	0	1	128	1	128	1.12e-76	223
MS.gene62426.t1	AT5G20160	92.969	128	9	0	1	128	1	128	4.77e-76	221
MS.gene62426.t1	AT4G12600	89.844	128	13	0	1	128	1	128	1.26e-72	213
MS.gene62426.t1	AT5G20160	74.375	160	9	1	1	128	1	160	3.79e-69	205
MS.gene62426.t1	AT4G12600	55.738	183	26	2	1	128	1	183	2.11e-49	156
MS.gene62426.t1	AT5G20160	65.741	108	5	1	1	76	1	108	1.11e-31	108
MS.gene62426.t1	AT5G08180	35.938	128	73	1	8	126	24	151	3.66e-21	83.6
MS.gene62426.t1	AT5G08180	35.938	128	73	1	8	126	24	151	3.66e-21	83.6

Find 29 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACTATAAGCAACTCAAAA+AGG	0.236397	8.2:-14259812	MS.gene62426:CDS
TATTTCTGAATTTGTTGTTA+TGG	0.302213	8.2:-14259667	MS.gene62426:CDS
TTGTTGAACAAGATCCATTA+TGG	0.342840	8.2:+14259840	None:intergenic
TCCGTTACTTGCTGAGGATA+AGG	0.383122	8.2:-14259607	MS.gene62426:intron
AGCTACTAAGACCTTGAATA+GGG	0.384258	8.2:-14259691	MS.gene62426:CDS
TCAGCCAAAGGGTATGCCTT+AGG	0.396497	8.2:+14259880	None:intergenic
CAGCTACTAAGACCTTGAAT+AGG	0.416543	8.2:-14259692	MS.gene62426:CDS
TTCAGAAATACCCCTATTCA+AGG	0.432400	8.2:+14259680	None:intergenic
TAACCCTAAGGCATACCCTT+TGG	0.467615	8.2:-14259884	MS.gene62426:CDS
TCAGCTAACAATCACCATAA+TGG	0.473714	8.2:-14259854	MS.gene62426:CDS
CAGCCAAAGGGTATGCCTTA+GGG	0.474338	8.2:+14259881	None:intergenic
CCTCAGCAAGTAACGGAAGA+TGG	0.475355	8.2:+14259613	None:intergenic
TGTGTTCGTCACATCAAAAC+AGG	0.479560	8.2:-14258849	MS.gene62426:CDS
CCATCTTCCGTTACTTGCTG+AGG	0.512018	8.2:-14259613	MS.gene62426:CDS
GTCACATCAAAACAGGCACT+TGG	0.523031	8.2:-14258842	MS.gene62426:CDS
AGAACAAGCAATCACTGGTC+GGG	0.539786	8.2:+14258802	None:intergenic
GTCACAGAACAAGCAATCAC+TGG	0.562045	8.2:+14258797	None:intergenic
TTTGATGTGACGAACACATA+GGG	0.566367	8.2:+14258854	None:intergenic
CAGGCACTTGGGCGTGCTTG+TGG	0.575874	8.2:-14258830	MS.gene62426:CDS
TTAGCTGAGCATCAGCCAAA+GGG	0.590874	8.2:+14259869	None:intergenic
ACCTTATCCTCAGCAAGTAA+CGG	0.593594	8.2:+14259606	None:intergenic
GTTAGCTGAGCATCAGCCAA+AGG	0.598758	8.2:+14259868	None:intergenic
GTCTCAGATTCAGCAACTCA+AGG	0.599622	8.2:-14258750	MS.gene62426:intron
TGTTCTGTGACAACTAATGA+AGG	0.613805	8.2:-14258785	MS.gene62426:CDS
CAGAACAAGCAATCACTGGT+CGG	0.618383	8.2:+14258801	None:intergenic
TGGAGAAGCAGTTAACCCTA+AGG	0.621032	8.2:-14259896	MS.gene62426:CDS
TCACATCAAAACAGGCACTT+GGG	0.623134	8.2:-14258841	MS.gene62426:CDS
AGATGGAGAAGAATCTCAAG+AGG	0.644746	8.2:+14259630	None:intergenic
GCTACTAAGACCTTGAATAG+GGG	0.659558	8.2:-14259690	MS.gene62426:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAATGAATAAGAAATTA+GGG	+	chr8.2:14258864-14258883	None:intergenic	10.0%
!!	AATATTGAAATAAAATGAAT+CGG	+	chr8.2:14259685-14259704	None:intergenic	10.0%
!!	AATTTACACAATTTATAATA+AGG	-	chr8.2:14259331-14259350	MS.gene62426:intron	10.0%
!!!	ATTTTATTTCAATATTTTTC+TGG	-	chr8.2:14259689-14259708	MS.gene62426:CDS	10.0%
!!	ATTAACAAGAAGATATAAAA+CGG	+	chr8.2:14258627-14258646	None:intergenic	15.0%
!!	ATTAATCAATCAATAATGAA+AGG	+	chr8.2:14258905-14258924	None:intergenic	15.0%
!!	CAAAAAATGAATAAGAAATT+AGG	+	chr8.2:14258865-14258884	None:intergenic	15.0%
!!	CAAATTAAGAGTTAAACATA+GGG	+	chr8.2:14258654-14258673	None:intergenic	20.0%
!!	GAAAATGAATTCTGAATATT+TGG	-	chr8.2:14259978-14259997	MS.gene62426:intron	20.0%
!!	GTGTAAATTAGTATTAGTAT+AGG	+	chr8.2:14259320-14259339	None:intergenic	20.0%
!!	TCAAATTAAGAGTTAAACAT+AGG	+	chr8.2:14258655-14258674	None:intergenic	20.0%
!!!	ATTTTAGTTTTTTCATGACT+TGG	-	chr8.2:14259148-14259167	MS.gene62426:intron	20.0%
!!!	TATTTCTGAATTTGTTGTTA+TGG	-	chr8.2:14258967-14258986	MS.gene62426:intron	20.0%
!	AACTATAAGCAACTCAAAAA+GGG	-	chr8.2:14258823-14258842	MS.gene62426:CDS	25.0%
!	AGTAATATTACTTGAAGACA+TGG	+	chr8.2:14259563-14259582	None:intergenic	25.0%
!	ATAAAATGAATCGGATGAAA+TGG	+	chr8.2:14259676-14259695	None:intergenic	25.0%
!	ATGAATTCTGAATATTTGGT+TGG	-	chr8.2:14259982-14260001	MS.gene62426:intron	25.0%
!	TAACTATAAGCAACTCAAAA+AGG	-	chr8.2:14258822-14258841	MS.gene62426:CDS	25.0%
!!	TGTAGCTTATTGTTATTGTT+AGG	-	chr8.2:14259427-14259446	MS.gene62426:intron	25.0%
	ATGATGAAAAATTTGCAGAC+TGG	-	chr8.2:14258718-14258737	MS.gene62426:intron	30.0%
	ATGCTATGCATAACAAATAG+CGG	+	chr8.2:14259248-14259267	None:intergenic	30.0%
	TGTTATGCATAGCATCTTAT+TGG	-	chr8.2:14259253-14259272	MS.gene62426:intron	30.0%
	TTGTATCCATACAGATGTTT+GGG	-	chr8.2:14259285-14259304	MS.gene62426:intron	30.0%
	TTGTTGAACAAGATCCATTA+TGG	+	chr8.2:14258797-14258816	None:intergenic	30.0%
	TTTGTATCCATACAGATGTT+TGG	-	chr8.2:14259284-14259303	MS.gene62426:intron	30.0%
!!!	TGACTTTTAAAACAGGGTTT+TGG	-	chr8.2:14259380-14259399	MS.gene62426:intron	30.0%
	AGCTACTAAGACCTTGAATA+GGG	-	chr8.2:14258943-14258962	MS.gene62426:intron	35.0%
	CATGGTTTATCACACTTGTT+TGG	+	chr8.2:14259545-14259564	None:intergenic	35.0%
	CTTGACATTTCAAAGCAACT+AGG	+	chr8.2:14259456-14259475	None:intergenic	35.0%
	GCTGAGGATAAGGTATATTA+GGG	-	chr8.2:14259037-14259056	MS.gene62426:intron	35.0%
	TCAGCTAACAATCACCATAA+TGG	-	chr8.2:14258780-14258799	MS.gene62426:CDS	35.0%
	TGCTATGCATAACAAATAGC+GGG	+	chr8.2:14259247-14259266	None:intergenic	35.0%
	TGCTGAGGATAAGGTATATT+AGG	-	chr8.2:14259036-14259055	MS.gene62426:intron	35.0%
	TGTTCTGTGACAACTAATGA+AGG	-	chr8.2:14259849-14259868	MS.gene62426:CDS	35.0%
	TTCAGAAATACCCCTATTCA+AGG	+	chr8.2:14258957-14258976	None:intergenic	35.0%
	TTTGATGTGACGAACACATA+GGG	+	chr8.2:14259783-14259802	None:intergenic	35.0%
!	TGAATATTTGGTTGGTATGC+AGG	-	chr8.2:14259990-14260009	MS.gene62426:intron	35.0%
!	TTTTGATGTGACGAACACAT+AGG	+	chr8.2:14259784-14259803	None:intergenic	35.0%
!!	CTAGTTGCTTTGAAATGTCA+AGG	-	chr8.2:14259454-14259473	MS.gene62426:intron	35.0%
!!!	GATCGCTGACTTTTAAAACA+GGG	-	chr8.2:14259374-14259393	MS.gene62426:intron	35.0%
!!!	TGATCGCTGACTTTTAAAAC+AGG	-	chr8.2:14259373-14259392	MS.gene62426:intron	35.0%
	ACCTTATCCTCAGCAAGTAA+CGG	+	chr8.2:14259031-14259050	None:intergenic	40.0%
	AGATGGAGAAGAATCTCAAG+AGG	+	chr8.2:14259007-14259026	None:intergenic	40.0%
	ATCGAGACCTAACAACATTC+AGG	+	chr8.2:14259497-14259516	None:intergenic	40.0%
	CAGCTACTAAGACCTTGAAT+AGG	-	chr8.2:14258942-14258961	MS.gene62426:intron	40.0%
	GCTACTAAGACCTTGAATAG+GGG	-	chr8.2:14258944-14258963	MS.gene62426:intron	40.0%
	GCTATGCATAACAAATAGCG+GGG	+	chr8.2:14259246-14259265	None:intergenic	40.0%
	TCACATCAAAACAGGCACTT+GGG	-	chr8.2:14259793-14259812	MS.gene62426:intron	40.0%
	TCGAGACCTAACAACATTCA+GGG	+	chr8.2:14259496-14259515	None:intergenic	40.0%
	TCTTTCCCCTGAATGTTGTT+AGG	-	chr8.2:14259487-14259506	MS.gene62426:intron	40.0%
	TGTGTTCGTCACATCAAAAC+AGG	-	chr8.2:14259785-14259804	MS.gene62426:intron	40.0%
!!	CTCAAAAAGGGTGCTAATGA+AGG	-	chr8.2:14258835-14258854	MS.gene62426:CDS	40.0%
	AGAACAAGCAATCACTGGTC+GGG	+	chr8.2:14259835-14259854	None:intergenic	45.0%
	CAGAACAAGCAATCACTGGT+CGG	+	chr8.2:14259836-14259855	None:intergenic	45.0%
	CCACAACCCAAACATCTGTA+TGG	+	chr8.2:14259294-14259313	None:intergenic	45.0%
	CGAGACCTAACAACATTCAG+GGG	+	chr8.2:14259495-14259514	None:intergenic	45.0%
	GTCACAGAACAAGCAATCAC+TGG	+	chr8.2:14259840-14259859	None:intergenic	45.0%
	GTCACATCAAAACAGGCACT+TGG	-	chr8.2:14259792-14259811	MS.gene62426:intron	45.0%
	GTCTCAGATTCAGCAACTCA+AGG	-	chr8.2:14259884-14259903	MS.gene62426:CDS	45.0%
	TAACCCTAAGGCATACCCTT+TGG	-	chr8.2:14258750-14258769	MS.gene62426:intron	45.0%
	TGGAGAAGCAGTTAACCCTA+AGG	-	chr8.2:14258738-14258757	MS.gene62426:intron	45.0%
	TTAGCTGAGCATCAGCCAAA+GGG	+	chr8.2:14258768-14258787	None:intergenic	45.0%
!	TCCGTTACTTGCTGAGGATA+AGG	-	chr8.2:14259027-14259046	MS.gene62426:intron	45.0%
!!	CCATACAGATGTTTGGGTTG+TGG	-	chr8.2:14259291-14259310	MS.gene62426:intron	45.0%
	GTTAGCTGAGCATCAGCCAA+AGG	+	chr8.2:14258769-14258788	None:intergenic	50.0%
!	CAGCCAAAGGGTATGCCTTA+GGG	+	chr8.2:14258756-14258775	None:intergenic	50.0%
!	CCATCTTCCGTTACTTGCTG+AGG	-	chr8.2:14259021-14259040	MS.gene62426:intron	50.0%
!	CCTCAGCAAGTAACGGAAGA+TGG	+	chr8.2:14259024-14259043	None:intergenic	50.0%
!	TCAGCCAAAGGGTATGCCTT+AGG	+	chr8.2:14258757-14258776	None:intergenic	50.0%
	CAGGCACTTGGGCGTGCTTG+TGG	-	chr8.2:14259804-14259823	MS.gene62426:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	14258618	14260038	14258618	ID=MS.gene62426
chr8.2	mRNA	14258618	14260038	14258618	ID=MS.gene62426.t1;Parent=MS.gene62426
chr8.2	exon	14260036	14260038	14260036	ID=MS.gene62426.t1.exon1;Parent=MS.gene62426.t1
chr8.2	CDS	14260036	14260038	14260036	ID=cds.MS.gene62426.t1;Parent=MS.gene62426.t1
chr8.2	exon	14259800	14259920	14259800	ID=MS.gene62426.t1.exon2;Parent=MS.gene62426.t1
chr8.2	CDS	14259800	14259920	14259800	ID=cds.MS.gene62426.t1;Parent=MS.gene62426.t1
chr8.2	exon	14259608	14259711	14259608	ID=MS.gene62426.t1.exon3;Parent=MS.gene62426.t1
chr8.2	CDS	14259608	14259711	14259608	ID=cds.MS.gene62426.t1;Parent=MS.gene62426.t1
chr8.2	exon	14258751	14258882	14258751	ID=MS.gene62426.t1.exon4;Parent=MS.gene62426.t1
chr8.2	CDS	14258751	14258882	14258751	ID=cds.MS.gene62426.t1;Parent=MS.gene62426.t1
chr8.2	exon	14258618	14258644	14258618	ID=MS.gene62426.t1.exon5;Parent=MS.gene62426.t1
chr8.2	CDS	14258618	14258644	14258618	ID=cds.MS.gene62426.t1;Parent=MS.gene62426.t1

Gene Sequence

>MS.gene62426

ATGGTACCGTTTTATATCTTCTTGTTAATTTTTCCCTATGTTTAACTCTTAATTTGATTCAGTGTTAATTGATTACTGATTAATACTTGTTTTTTTGTATATGATGAAAAATTTGCAGACTGGAGAAGCAGTTAACCCTAAGGCATACCCTTTGGCTGATGCTCAGCTAACAATCACCATAATGGATCTTGTTCAACAAGCTGCTAACTATAAGCAACTCAAAAAGGGTGCTAATGAAGGTAACCCTAATTTCTTATTCATTTTTTGTTTAATGTCTTTTTTGTCCTTTCATTATTGATTGATTAATTGATTTTTGTTGTGTTGCAGCTACTAAGACCTTGAATAGGGGTATTTCTGAATTTGTTGTTATGGCTGCTGATGCTGAACCTCTTGAGATTCTTCTCCATCTTCCGTTACTTGCTGAGGATAAGGTATATTAGGGTTTTGTTTATGTAATGTATGATCTTCATATAATGAAATGAAATGACTAGCTATGTTAACTAAATCTGTCACAAATTAAAAGTTAAACAATTTTAGTTTTTTCATGACTTGGAAATAAATGTTTTATTTGTGAATATATATGATTATGCTATGCTTTTTCGTAGTTCACAAACAAAGAAATCGACCCCGCTATTTGTTATGCATAGCATCTTATTGGTACAATCATTTGTATCCATACAGATGTTTGGGTTGTGGTCTCCTATACTAATACTAATTTACACAATTTATAATAAGGCTGTAATTGCATTTGCTGCTGATCGCTGACTTTTAAAACAGGGTTTTGGTATGTTTGTGTTATGATATGTTTATGTAGCTTATTGTTATTGTTAGGTAGCCTAGTTGCTTTGAAATGTCAAGGTTGTGAAATTTCTTTCCCCTGAATGTTGTTAGGTCTCGATGAGTTTGTTGTAACTTACTCTTAAACCAAACAAGTGTGATAAACCATGTCTTCAAGTAATATTACTATGAACTGTCACAGTATCAGTATAGTTTTTGTCTCTGCTAATCTTTCTTGCGTGTGTTGCATTGCAATTTGTAAATGTATTTTGTCTTAGCCATTTCATCCGATTCATTTTATTTCAATATTTTTCTGGTTTTTGTCTATCAGCAATGTATAGATTATTAATCATGTGCACTATCTGTTTGAAATTTTTAGAATGTTCCCTATGTGTTCGTCACATCAAAACAGGCACTTGGGCGTGCTTGTGGTGTCACCCGACCAGTGATTGCTTGTTCTGTGACAACTAATGAAGGTAGTCAATTGAAGTCTCAGATTCAGCAACTCAAGGTATGCACTATGCTTCGTTTTCATGATTTTCATTTTCACTATTTATTGTAATTGTTATATGTTACTTTAATTGAAAATGAATTCTGAATATTTGGTTGGTATGCAGGATGCTATTGAGAAGCTGCTAATCTGA

Protein sequence

>MS.gene62426.t1

MTGEAVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVTSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI