AlfalfaGEDB | MS.gene61498

M. sativa cultivar XinJiangDaYe / MS.gene61498

KEGG: K12845
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005730 GO:0008150 GO:0022613 GO:0032991 GO:0042254 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0071840 GO:0097159 GO:0110165 GO:1901363 GO:1990904
PFAM: PF01248.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61498.t1	XP_003609312.1	100	128	0	0	1	128	1	128	3.90E-62	247.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61498.t1	Q5XH16	82.0	128	23	0	1	128	1	128	1.3e-53	210.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61498.t1	B7FKG0	100.0	128	0	0	1	128	1	128	2.8e-62	247.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene61498	MS.gene85342	PPI
MS.gene059422	MS.gene61498	PPI
MS.gene036800	MS.gene61498	PPI
MS.gene61498	MS.gene038837	PPI
MS.gene61498	MS.gene002484	PPI
MS.gene61498	MS.gene25903	PPI
MS.gene61498	MS.gene001986	PPI
MS.gene61498	MS.gene062116	PPI
MS.gene59047	MS.gene61498	PPI
MS.gene61498	MS.gene01456	PPI
MS.gene61498	MS.gene23314	PPI
MS.gene61498	MS.gene057157	PPI
MS.gene61498	MS.gene009509	PPI
MS.gene61498	MS.gene92650	PPI
MS.gene61498	MS.gene28172	PPI
MS.gene074400	MS.gene61498	PPI
MS.gene033349	MS.gene61498	PPI
MS.gene61498	MS.gene041557	PPI
MS.gene61498	MS.gene41916	PPI
MS.gene033348	MS.gene61498	PPI
MS.gene61498	MS.gene070139	PPI
MS.gene61498	MS.gene51967	PPI
MS.gene61498	MS.gene065463	PPI
MS.gene011390	MS.gene61498	PPI
MS.gene61498	MS.gene68979	PPI
MS.gene61498	MS.gene70579	PPI
MS.gene63427	MS.gene61498	PPI
MS.gene61498	MS.gene054186	PPI
MS.gene61498	MS.gene015586	PPI
MS.gene61498	MS.gene056257	PPI
MS.gene61498	MS.gene019309	PPI
MS.gene002484	MS.gene61498	PPI
MS.gene61498	MS.gene044498	PPI
MS.gene61498	MS.gene067362	PPI
MS.gene61498	MS.gene51964	PPI
MS.gene61498	MS.gene035331	PPI
MS.gene038837	MS.gene61498	PPI
MS.gene61498	MS.gene053443	PPI
MS.gene61498	MS.gene030697	PPI
MS.gene61498	MS.gene62426	PPI
MS.gene61498	MS.gene047337	PPI
MS.gene61498	MS.gene037324	PPI
MS.gene01456	MS.gene61498	PPI
MS.gene61498	MS.gene58148	PPI
MS.gene68979	MS.gene61498	PPI
MS.gene61498	MS.gene045843	PPI
MS.gene045843	MS.gene61498	PPI
MS.gene61498	MS.gene01057	PPI
MS.gene61498	MS.gene036487	PPI
MS.gene95934	MS.gene61498	PPI
MS.gene61498	MS.gene59319	PPI
MS.gene53517	MS.gene61498	PPI
MS.gene51687	MS.gene61498	PPI
MS.gene61498	MS.gene70292	PPI
MS.gene61498	MS.gene022249	PPI
MS.gene61498	MS.gene033017	PPI
MS.gene61498	MS.gene50744	PPI
MS.gene61498	MS.gene072782	PPI
MS.gene70551	MS.gene61498	PPI
MS.gene61498	MS.gene073888	PPI
MS.gene61498	MS.gene010453	PPI
MS.gene61498	MS.gene019081	PPI
MS.gene61498	MS.gene99744	PPI
MS.gene61498	MS.gene003693	PPI
MS.gene61498	MS.gene025347	PPI
MS.gene61498	MS.gene065462	PPI
MS.gene61498	MS.gene029242	PPI
MS.gene047734	MS.gene61498	PPI
MS.gene61498	MS.gene038800	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61498.t1	MTR_4g114330	100.000	128	0	0	1	128	1	128	1.64e-91	260
MS.gene61498.t1	MTR_0011s0330	97.656	128	3	0	1	128	1	128	3.85e-89	254
MS.gene61498.t1	MTR_4g112780	34.483	116	75	1	4	118	17	132	1.45e-17	73.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61498.t1	AT4G22380	93.750	128	8	0	1	128	1	128	1.12e-76	223
MS.gene61498.t1	AT5G20160	92.969	128	9	0	1	128	1	128	4.77e-76	221
MS.gene61498.t1	AT4G12600	89.844	128	13	0	1	128	1	128	1.26e-72	213
MS.gene61498.t1	AT5G20160	74.375	160	9	1	1	128	1	160	3.79e-69	205
MS.gene61498.t1	AT4G12600	55.738	183	26	2	1	128	1	183	2.11e-49	156
MS.gene61498.t1	AT5G20160	65.741	108	5	1	1	76	1	108	1.11e-31	108
MS.gene61498.t1	AT5G08180	35.938	128	73	1	8	126	24	151	3.66e-21	83.6
MS.gene61498.t1	AT5G08180	35.938	128	73	1	8	126	24	151	3.66e-21	83.6

Find 27 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACTATAAGCAACTCAAAA+AGG	0.236397	8.3:-13260678	MS.gene61498:CDS
TATTTCTGAATTTGTTGTTA+TGG	0.302213	8.3:-13260532	MS.gene61498:CDS
TTGTTGAACAAGATCCATTA+TGG	0.342840	8.3:+13260706	None:intergenic
AGCTACTAAGACCTTGAATA+GGG	0.384258	8.3:-13260556	MS.gene61498:CDS
TCAGCCAAAGGGTATGCCTT+AGG	0.396497	8.3:+13260746	None:intergenic
TCCTCTACTTGCTGAGGATA+AGG	0.406375	8.3:-13260472	MS.gene61498:intron
CAGCTACTAAGACCTTGAAT+AGG	0.416543	8.3:-13260557	MS.gene61498:CDS
TTCAGAAATACCCCTATTCA+AGG	0.432400	8.3:+13260545	None:intergenic
TAACCCTAAGGCATACCCTT+TGG	0.467615	8.3:-13260750	MS.gene61498:CDS
TCAGCTAACAATCACCATAA+TGG	0.473714	8.3:-13260720	MS.gene61498:CDS
CAGCCAAAGGGTATGCCTTA+GGG	0.474338	8.3:+13260747	None:intergenic
CCTCAGCAAGTAGAGGAAGA+TGG	0.517598	8.3:+13260478	None:intergenic
AGAACAAGCAATCACTGGTC+TGG	0.520395	8.3:+13259646	None:intergenic
CCATCTTCCTCTACTTGCTG+AGG	0.525035	8.3:-13260478	MS.gene61498:CDS
GTCACATCAAAACAAGCACT+TGG	0.533789	8.3:-13259686	MS.gene61498:CDS
TTTGATGTGACGAACACATA+GGG	0.556996	8.3:+13259698	None:intergenic
GTCACAGAACAAGCAATCAC+TGG	0.559563	8.3:+13259641	None:intergenic
AGGAGAAGCAGTTAACCCTA+AGG	0.573343	8.3:-13260762	MS.gene61498:CDS
CAAGCACTTGGGCGTGCTTG+TGG	0.582426	8.3:-13259674	MS.gene61498:CDS
TTAGCTGAGCATCAGCCAAA+GGG	0.590874	8.3:+13260735	None:intergenic
AGATGGAGAAGAATCTCAAG+AGG	0.597462	8.3:+13260495	None:intergenic
GTTAGCTGAGCATCAGCCAA+AGG	0.598758	8.3:+13260734	None:intergenic
GTCTCAGATTCAGCAACTCA+AGG	0.599622	8.3:-13259594	MS.gene61498:intron
ACCTTATCCTCAGCAAGTAG+AGG	0.612122	8.3:+13260471	None:intergenic
TGTTCTGTGACAACTAATGA+AGG	0.613805	8.3:-13259629	MS.gene61498:CDS
GCTACTAAGACCTTGAATAG+GGG	0.659558	8.3:-13260555	MS.gene61498:CDS
TCACATCAAAACAAGCACTT+GGG	0.661984	8.3:-13259685	MS.gene61498:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAATGAAATAAGAAATT+AGG	+	chr8.3:13259708-13259727	None:intergenic	10.0%
!!	AAAAATGAAATAAGAAATTA+GGG	+	chr8.3:13259707-13259726	None:intergenic	10.0%
!!	AATATTGAAATAAAATGAAT+CGG	+	chr8.3:13260550-13260569	None:intergenic	10.0%
!!	AATTTACACAATTTATAATA+AGG	-	chr8.3:13260194-13260213	MS.gene61498:intron	10.0%
!!!	ATTTTATTTCAATATTTTTC+CGG	-	chr8.3:13260554-13260573	MS.gene61498:CDS	10.0%
!!	ATTAACAAGAAGATATAAAA+CGG	+	chr8.3:13259471-13259490	None:intergenic	15.0%
!!	ATTAATCAATCAATAATCAA+AGG	+	chr8.3:13259749-13259768	None:intergenic	15.0%
!!	TAAATTGTGTAAATTAGTAT+AGG	+	chr8.3:13260189-13260208	None:intergenic	15.0%
!!!	ATTTTAGTTTTTTCATAACT+TGG	-	chr8.3:13260017-13260036	MS.gene61498:intron	15.0%
!!	ATTGTTAAAAAATAAGAGCT+TGG	+	chr8.3:13259999-13260018	None:intergenic	20.0%
!!	CAAATTAAGAGTTAAACATA+GGG	+	chr8.3:13259498-13259517	None:intergenic	20.0%
!!	GAAAATGAATTCTGAATATT+TGG	-	chr8.3:13260843-13260862	MS.gene61498:intron	20.0%
!!	TCAAATTAAGAGTTAAACAT+AGG	+	chr8.3:13259499-13259518	None:intergenic	20.0%
!!!	TATTTCTGAATTTGTTGTTA+TGG	-	chr8.3:13259811-13259830	MS.gene61498:intron	20.0%
!	AACTATAAGCAACTCAAAAA+GGG	-	chr8.3:13259666-13259685	MS.gene61498:CDS	25.0%
!	AGTAATATTACTTGAAGACA+TGG	+	chr8.3:13260428-13260447	None:intergenic	25.0%
!	ATAAAATGAATCGGATGAAA+TGG	+	chr8.3:13260541-13260560	None:intergenic	25.0%
!	ATGAATTCTGAATATTTGGT+TGG	-	chr8.3:13260847-13260866	MS.gene61498:intron	25.0%
!	TAACTATAAGCAACTCAAAA+AGG	-	chr8.3:13259665-13259684	MS.gene61498:CDS	25.0%
!!!	TGTTATGCATAGCATTTTTT+TGG	-	chr8.3:13260122-13260141	MS.gene61498:intron	25.0%
	AAACAGGGTTATGTTATGTT+TGG	-	chr8.3:13260234-13260253	MS.gene61498:intron	30.0%
	AACAGGGTTATGTTATGTTT+GGG	-	chr8.3:13260235-13260254	MS.gene61498:intron	30.0%
	TTGTATCCATACAGATGTTT+GGG	-	chr8.3:13260154-13260173	MS.gene61498:intron	30.0%
	TTGTTGAACAAGATCCATTA+TGG	+	chr8.3:13259640-13259659	None:intergenic	30.0%
	TTTGTATCCATACAGATGTT+TGG	-	chr8.3:13260153-13260172	MS.gene61498:intron	30.0%
!	ATGATGAAAATTTTGCAGAC+AGG	-	chr8.3:13259561-13259580	MS.gene61498:intron	30.0%
!!!	GATCGATGACTTTTAAAACA+GGG	-	chr8.3:13260219-13260238	MS.gene61498:intron	30.0%
!!!	TGATCGATGACTTTTAAAAC+AGG	-	chr8.3:13260218-13260237	MS.gene61498:intron	30.0%
	AGCTACTAAGACCTTGAATA+GGG	-	chr8.3:13259787-13259806	MS.gene61498:intron	35.0%
	CATGGTTTATCACACTTGTT+TGG	+	chr8.3:13260410-13260429	None:intergenic	35.0%
	CATTGCTGATAGACAAAAAC+CGG	+	chr8.3:13260576-13260595	None:intergenic	35.0%
	CTTGACATTTCAAAGCAACT+AGG	+	chr8.3:13260301-13260320	None:intergenic	35.0%
	GCTATGCATAACAAATAGCA+GGG	+	chr8.3:13260115-13260134	None:intergenic	35.0%
	GCTGAGGATAAGGTATATTA+GGG	-	chr8.3:13259881-13259900	MS.gene61498:intron	35.0%
	TCACATCAAAACAAGCACTT+GGG	-	chr8.3:13260658-13260677	MS.gene61498:intron	35.0%
	TCAGCTAACAATCACCATAA+TGG	-	chr8.3:13259623-13259642	MS.gene61498:CDS	35.0%
	TGCTATGCATAACAAATAGC+AGG	+	chr8.3:13260116-13260135	None:intergenic	35.0%
	TGCTGAGGATAAGGTATATT+AGG	-	chr8.3:13259880-13259899	MS.gene61498:intron	35.0%
	TGTTCTGTGACAACTAATGA+AGG	-	chr8.3:13260714-13260733	MS.gene61498:CDS	35.0%
	TTCAGAAATACCCCTATTCA+AGG	+	chr8.3:13259801-13259820	None:intergenic	35.0%
	TTTGATGTGACGAACACATA+GGG	+	chr8.3:13260648-13260667	None:intergenic	35.0%
!	TGAATATTTGGTTGGTATGC+AGG	-	chr8.3:13260855-13260874	MS.gene61498:intron	35.0%
!	TTTTGATGTGACGAACACAT+AGG	+	chr8.3:13260649-13260668	None:intergenic	35.0%
!!	CTAGTTGCTTTGAAATGTCA+AGG	-	chr8.3:13260299-13260318	MS.gene61498:intron	35.0%
	ACAACATTCAGGGGAAAGAT+AGG	+	chr8.3:13260351-13260370	None:intergenic	40.0%
	AGATGGAGAAGAATCTCAAG+AGG	+	chr8.3:13259851-13259870	None:intergenic	40.0%
	ATCGAGACCTAACAACATTC+AGG	+	chr8.3:13260362-13260381	None:intergenic	40.0%
	CAGCTACTAAGACCTTGAAT+AGG	-	chr8.3:13259786-13259805	MS.gene61498:intron	40.0%
	GCTACTAAGACCTTGAATAG+GGG	-	chr8.3:13259788-13259807	MS.gene61498:intron	40.0%
	GTCACATCAAAACAAGCACT+TGG	-	chr8.3:13260657-13260676	MS.gene61498:intron	40.0%
	TCGAGACCTAACAACATTCA+GGG	+	chr8.3:13260361-13260380	None:intergenic	40.0%
	TCTTTCCCCTGAATGTTGTT+AGG	-	chr8.3:13260352-13260371	MS.gene61498:intron	40.0%
!!	CTCAAAAAGGGTGCTAATGA+AGG	-	chr8.3:13259678-13259697	MS.gene61498:CDS	40.0%
	ACCTTATCCTCAGCAAGTAG+AGG	+	chr8.3:13259875-13259894	None:intergenic	45.0%
	AGAACAAGCAATCACTGGTC+TGG	+	chr8.3:13260700-13260719	None:intergenic	45.0%
	AGGAGAAGCAGTTAACCCTA+AGG	-	chr8.3:13259581-13259600	MS.gene61498:intron	45.0%
	CCACAACCCAAACATCTGTA+TGG	+	chr8.3:13260163-13260182	None:intergenic	45.0%
	CGAGACCTAACAACATTCAG+GGG	+	chr8.3:13260360-13260379	None:intergenic	45.0%
	GTCACAGAACAAGCAATCAC+TGG	+	chr8.3:13260705-13260724	None:intergenic	45.0%
	GTCTCAGATTCAGCAACTCA+AGG	-	chr8.3:13260749-13260768	MS.gene61498:CDS	45.0%
	TAACCCTAAGGCATACCCTT+TGG	-	chr8.3:13259593-13259612	MS.gene61498:intron	45.0%
	TTAGCTGAGCATCAGCCAAA+GGG	+	chr8.3:13259611-13259630	None:intergenic	45.0%
!	TCCTCTACTTGCTGAGGATA+AGG	-	chr8.3:13259871-13259890	MS.gene61498:intron	45.0%
!!	CCATACAGATGTTTGGGTTG+TGG	-	chr8.3:13260160-13260179	MS.gene61498:intron	45.0%
	CCTCAGCAAGTAGAGGAAGA+TGG	+	chr8.3:13259868-13259887	None:intergenic	50.0%
	GTTAGCTGAGCATCAGCCAA+AGG	+	chr8.3:13259612-13259631	None:intergenic	50.0%
!	CAGCCAAAGGGTATGCCTTA+GGG	+	chr8.3:13259599-13259618	None:intergenic	50.0%
!	CCATCTTCCTCTACTTGCTG+AGG	-	chr8.3:13259865-13259884	MS.gene61498:intron	50.0%
!	TCAGCCAAAGGGTATGCCTT+AGG	+	chr8.3:13259600-13259619	None:intergenic	50.0%
	CAAGCACTTGGGCGTGCTTG+TGG	-	chr8.3:13260669-13260688	MS.gene61498:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	13259462	13260903	13259462	ID=MS.gene61498
chr8.3	mRNA	13259462	13260903	13259462	ID=MS.gene61498.t1;Parent=MS.gene61498
chr8.3	exon	13260901	13260903	13260901	ID=MS.gene61498.t1.exon1;Parent=MS.gene61498.t1
chr8.3	CDS	13260901	13260903	13260901	ID=cds.MS.gene61498.t1;Parent=MS.gene61498.t1
chr8.3	exon	13260666	13260786	13260666	ID=MS.gene61498.t1.exon2;Parent=MS.gene61498.t1
chr8.3	CDS	13260666	13260786	13260666	ID=cds.MS.gene61498.t1;Parent=MS.gene61498.t1
chr8.3	exon	13260473	13260576	13260473	ID=MS.gene61498.t1.exon3;Parent=MS.gene61498.t1
chr8.3	CDS	13260473	13260576	13260473	ID=cds.MS.gene61498.t1;Parent=MS.gene61498.t1
chr8.3	exon	13259595	13259726	13259595	ID=MS.gene61498.t1.exon4;Parent=MS.gene61498.t1
chr8.3	CDS	13259595	13259726	13259595	ID=cds.MS.gene61498.t1;Parent=MS.gene61498.t1
chr8.3	exon	13259462	13259488	13259462	ID=MS.gene61498.t1.exon5;Parent=MS.gene61498.t1
chr8.3	CDS	13259462	13259488	13259462	ID=cds.MS.gene61498.t1;Parent=MS.gene61498.t1

Gene Sequence

>MS.gene61498

ATGGTACCGTTTTATATCTTCTTGTTAATTTTTCCCTATGTTTAACTCTTAATTTGATTCAGTGCTAATTGATTACTGATTAATACTTATTTTTTGTATATGATGAAAATTTTGCAGACAGGAGAAGCAGTTAACCCTAAGGCATACCCTTTGGCTGATGCTCAGCTAACAATCACCATAATGGATCTTGTTCAACAAGCTGCTAACTATAAGCAACTCAAAAAGGGTGCTAATGAAGGTAACCCTAATTTCTTATTTCATTTTTTGTTTAATGTCTTTTTTGTCCTTTGATTATTGATTGATTAATTGATTTTTGTTGTGTTGCAGCTACTAAGACCTTGAATAGGGGTATTTCTGAATTTGTTGTTATGGCTGCTGATGCTGAACCTCTTGAGATTCTTCTCCATCTTCCTCTACTTGCTGAGGATAAGGTATATTAGGGTTTTGTTTATGTAATGTATGATCTTCATATAATGAAATGAAATGACTAGCTATGTTAACTGAATCTGTCACAAATTAAAACGATATAATACACCAAGCTCTTATTTTTTAACAATTTTAGTTTTTTCATAACTTGGAAATAAATGTTTTATTTGTGAATATATATGATTATGCTATGCTTTTTCGTAGTTCACAAACAAAGAAATCGACCCTGCTATTTGTTATGCATAGCATTTTTTTGGTACAATCATTTGTATCCATACAGATGTTTGGGTTGTGGTCTCCTATACTAATTTACACAATTTATAATAAGGCTGATCGATGACTTTTAAAACAGGGTTATGTTATGTTTGGGTTATGATATGTTTATGTAGCTTATTGTTATTGTTATGTAGCCTAGTTGCTTTGAAATGTCAAGGTTGTGAAATTTAGTGCTCATTCACAGCCTATCTTTCCCCTGAATGTTGTTAGGTCTCGATGAGTTTGTTGTAACTTACTCATAAACCAAACAAGTGTGATAAACCATGTCTTCAAGTAATATTACTATGAACTGTCACAGTATCAGTATAATTTTTGTCTCTGCTAATCTTTCTTGCGTGTGTTGCATTGCAATTTGTAAATGTATTTTGTCTTAGCCATTTCATCCGATTCATTTTATTTCAATATTTTTCCGGTTTTTGTCTATCAGCAATGTATAGATTATTAATCATGTGCACTATCTGTTTGAAATTTTTAGAATGTTCCCTATGTGTTCGTCACATCAAAACAAGCACTTGGGCGTGCTTGTGGTGTCACCAGACCAGTGATTGCTTGTTCTGTGACAACTAATGAAGGTAGTCAATTGAAGTCTCAGATTCAGCAACTCAAGGTATGCACTATGCTTCGTTTTCATGATTTTCATTTTCACTATTTATTGTAATTGTTATATGTTACTTTAATTGAAAATGAATTCTGAATATTTGGTTGGTATGCAGGATGCTATTGAGAAGCTGCTAATCTGA

Protein sequence

>MS.gene61498.t1

MTGEAVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVTSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI