AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene019081


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019081.t1 MTR_3g030055 86.928 153 15 2 1 153 8 155 1.15e-92 265
MS.gene019081.t1 MTR_3g030055 88.776 98 9 1 1 98 1 96 3.32e-58 177
MS.gene019081.t1 MTR_3g034200 46.795 156 72 4 6 151 2 156 3.88e-34 118
MS.gene019081.t1 MTR_3g050860 38.217 157 86 6 1 151 1 152 1.70e-17 78.2
MS.gene019081.t1 MTR_0439s0020 34.459 148 86 7 1 142 1 143 2.01e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019081.t1 AT5G13780 43.871 155 76 4 6 150 2 155 5.90e-30 107
MS.gene019081.t1 AT1G03150 28.070 114 78 3 35 146 39 150 1.72e-11 59.3

Find 39 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCACAATGTGCCAATATT+CGG 0.287513 3.4:+37411168 None:intergenic
CTCGTTACTCGCCCGAATAT+TGG 0.335099 3.4:-37411180 MS.gene019081:CDS
TGCATAAGAGTTGCAACATT+TGG 0.337938 3.4:-37411234 MS.gene019081:CDS
GAGCTGCAGAGTTGCTCTTC+CGG 0.347682 3.4:+37410279 None:intergenic
TCCACAATGTGCCAATATTC+GGG 0.349965 3.4:+37411169 None:intergenic
TCTTGGTTTGTTTCAAAAGA+TGG 0.379062 3.4:-37410258 MS.gene019081:CDS
TGCAACATTTGGAGACGTAT+TGG 0.394327 3.4:-37411223 MS.gene019081:CDS
GATAGGTATGGTGAACGAAT+AGG 0.421395 3.4:+37411040 None:intergenic
CTCCTCATCATCAAAATACT+TGG 0.430558 3.4:+37410211 None:intergenic
ATAGGTATGGTGAACGAATA+GGG 0.433258 3.4:+37411041 None:intergenic
CTCTGAAGGTGAAGAGGAAG+AGG 0.447607 3.4:-37411091 MS.gene019081:CDS
CTTGGTTTGTTTCAAAAGAT+GGG 0.449922 3.4:-37410257 MS.gene019081:CDS
CGCCCAAAACACCATGGAAA+AGG 0.453353 3.4:-37410983 MS.gene019081:intron
AAGAGCAACTCTGCAGCTCT+TGG 0.462439 3.4:-37410275 MS.gene019081:CDS
AAGACGTTTCAGTGCATGTC+CGG 0.496711 3.4:-37410298 MS.gene019081:CDS
CATTTGGAGACGTATTGGAT+TGG 0.499964 3.4:-37411218 MS.gene019081:CDS
GTTGCTTACCTCCTCTCTGA+AGG 0.508220 3.4:-37411105 MS.gene019081:CDS
AAGGTGAAGAGGAAGAGGAA+GGG 0.530803 3.4:-37411086 MS.gene019081:CDS
AGGAACAATGTCGTCAAAGA+AGG 0.539052 3.4:-37411259 None:intergenic
GTCTTGAGGAAGATACTCAA+GGG 0.554037 3.4:-37410179 MS.gene019081:CDS
GAAGGTGAAGAGGAAGAGGA+AGG 0.556577 3.4:-37411087 MS.gene019081:CDS
TGTCTTGAGGAAGATACTCA+AGG 0.562517 3.4:-37410180 MS.gene019081:CDS
CCTCCTCTCTGAAGGTGAAG+AGG 0.564442 3.4:-37411097 MS.gene019081:CDS
GCCCGAATATTGGCACATTG+TGG 0.568169 3.4:-37411170 MS.gene019081:CDS
TTACACACTTCACGAGGTGA+AGG 0.607853 3.4:-37410234 MS.gene019081:CDS
CCTCTTCACCTTCAGAGAGG+AGG 0.614706 3.4:+37411097 None:intergenic
AGGAGGACGCTATTGTCTTG+AGG 0.615172 3.4:-37410193 MS.gene019081:CDS
CACCGCCGCCCAAAACACCA+TGG 0.620119 3.4:-37410989 MS.gene019081:CDS
ATAGGGAGGCGATACTAAAG+TGG 0.620673 3.4:+37411058 None:intergenic
CACCATACCTATCGTAAACG+TGG 0.631082 3.4:-37411030 MS.gene019081:CDS
GAGTGGGTCTCACAGTTAGG+TGG 0.634645 3.4:+37410145 None:intergenic
CTTCCTCTTCACCTTCAGAG+AGG 0.638002 3.4:+37411094 None:intergenic
GAAGAAGCTTATTCATGTCG+CGG 0.645766 3.4:-37411148 MS.gene019081:CDS
TACCACGTTTACGATAGGTA+TGG 0.651983 3.4:+37411028 None:intergenic
AGCGATACCACGTTTACGAT+AGG 0.681782 3.4:+37411023 None:intergenic
TCTTGAGGAAGATACTCAAG+GGG 0.688967 3.4:-37410178 MS.gene019081:CDS
CATGTCGCGGACAATTGCAG+CGG 0.697708 3.4:-37411135 MS.gene019081:CDS
GATGGGTTACACACTTCACG+AGG 0.713948 3.4:-37410240 MS.gene019081:CDS
GGTATGGTGAACGAATAGGG+AGG 0.789435 3.4:+37411044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTGTTTTTTGGATT+AGG - chr3.4:37410478-37410497 MS.gene019081:intron 15.0%
!!! TCTGTTATAATTTTGTTTTT+TGG - chr3.4:37410472-37410491 MS.gene019081:intron 15.0%
!!! AGACTTTTAATGGAAAAAAT+AGG + chr3.4:37411059-37411078 None:intergenic 20.0%
!!! TTTCTTCTTGTTAATGATTT+TGG - chr3.4:37410852-37410871 MS.gene019081:intron 20.0%
! ACAACCAAAAACATCAAAAA+AGG + chr3.4:37411031-37411050 None:intergenic 25.0%
! GAAAACCAAAAGAAAAATCT+CGG + chr3.4:37410936-37410955 None:intergenic 25.0%
!! ACATTGTAACAGACTTTTAA+TGG + chr3.4:37411069-37411088 None:intergenic 25.0%
!! ATGGAGTTTGTTTAATGTTT+AGG - chr3.4:37410558-37410577 MS.gene019081:intron 25.0%
!!! TTTTCTTGTTTCCTAATCTT+GGG - chr3.4:37410951-37410970 MS.gene019081:intron 25.0%
ACAATGTGTAATTTGACTGA+AGG - chr3.4:37411082-37411101 MS.gene019081:CDS 30.0%
AGAAAACAGAAAATCATCAC+GGG + chr3.4:37410681-37410700 None:intergenic 30.0%
CAGAAAACAGAAAATCATCA+CGG + chr3.4:37410682-37410701 None:intergenic 30.0%
TAATGTTTAGGTATGAAGCT+TGG - chr3.4:37410570-37410589 MS.gene019081:intron 30.0%
TCCATACATAACCAGTTAAA+AGG + chr3.4:37410543-37410562 None:intergenic 30.0%
! CTTCTTGTTAATGATTTTGG+TGG - chr3.4:37410855-37410874 MS.gene019081:intron 30.0%
! TTTCTTGTTTCCTAATCTTG+GGG - chr3.4:37410952-37410971 MS.gene019081:intron 30.0%
!! CTTGGTTTGTTTCAAAAGAT+GGG - chr3.4:37411155-37411174 MS.gene019081:CDS 30.0%
!! GTTTTCTTGTTTCCTAATCT+TGG - chr3.4:37410950-37410969 MS.gene019081:intron 30.0%
!! TCTTGGTTTGTTTCAAAAGA+TGG - chr3.4:37411154-37411173 MS.gene019081:CDS 30.0%
!! TTGTTAATGATTTTGGTGGT+TGG - chr3.4:37410859-37410878 MS.gene019081:intron 30.0%
!!! CAGACCTTTTTTGATGTTTT+TGG - chr3.4:37411024-37411043 MS.gene019081:CDS 30.0%
AAAACACCATGGAAAAGGTT+AGG - chr3.4:37410434-37410453 MS.gene019081:intron 35.0%
AACCAAAAGAAAAATCTCGG+AGG + chr3.4:37410933-37410952 None:intergenic 35.0%
AATCTGAGTTTCACTATGCT+AGG - chr3.4:37410706-37410725 MS.gene019081:intron 35.0%
ATAGGTATGGTGAACGAATA+GGG + chr3.4:37410374-37410393 None:intergenic 35.0%
ATGTGTAATTTGACTGAAGG+TGG - chr3.4:37411085-37411104 MS.gene019081:CDS 35.0%
CTAGGATTATCTTCTATTCC+TGG - chr3.4:37410724-37410743 MS.gene019081:intron 35.0%
CTCCTCATCATCAAAATACT+TGG + chr3.4:37411204-37411223 None:intergenic 35.0%
TGCATAAGAGTTGCAACATT+TGG - chr3.4:37410178-37410197 MS.gene019081:CDS 35.0%
TGTGTAATTTGACTGAAGGT+GGG - chr3.4:37411086-37411105 MS.gene019081:CDS 35.0%
TTATCTTCTATTCCTGGAAG+TGG - chr3.4:37410730-37410749 MS.gene019081:intron 35.0%
TTCCACAATGTGCCAATATT+CGG + chr3.4:37410247-37410266 None:intergenic 35.0%
! AACAAACCTAACCTTTTCCA+TGG + chr3.4:37410443-37410462 None:intergenic 35.0%
! AACCTTTTCCATGGTGTTTT+GGG + chr3.4:37410434-37410453 None:intergenic 35.0%
! CGTGATGATTTTCTGTTTTC+TGG - chr3.4:37410680-37410699 MS.gene019081:intron 35.0%
! TAACCTTTTCCATGGTGTTT+TGG + chr3.4:37410435-37410454 None:intergenic 35.0%
! TTGGTTGAGTTGTTTCGTTA+GGG - chr3.4:37410589-37410608 MS.gene019081:intron 35.0%
! TTTTCAGATAGATCACACAG+TGG + chr3.4:37410658-37410677 None:intergenic 35.0%
!! GCCTTTTAACTGGTTATGTA+TGG - chr3.4:37410539-37410558 MS.gene019081:intron 35.0%
!! TTCTGTAGTTAGTCTTGTCT+GGG - chr3.4:37410895-37410914 MS.gene019081:intron 35.0%
!! TTTCTGTAGTTAGTCTTGTC+TGG - chr3.4:37410894-37410913 MS.gene019081:intron 35.0%
!!! CAAGTATTTTGATGATGAGG+AGG - chr3.4:37411202-37411221 MS.gene019081:CDS 35.0%
AAACACTTCCCACAGAGAAT+TGG + chr3.4:37410810-37410829 None:intergenic 40.0%
AAAGGGAGTGGCAATGATTA+GGG + chr3.4:37410770-37410789 None:intergenic 40.0%
CATTTGGAGACGTATTGGAT+TGG - chr3.4:37410194-37410213 MS.gene019081:CDS 40.0%
GAAGAAGCTTATTCATGTCG+CGG - chr3.4:37410264-37410283 MS.gene019081:CDS 40.0%
GATAGGTATGGTGAACGAAT+AGG + chr3.4:37410375-37410394 None:intergenic 40.0%
GTCTTGAGGAAGATACTCAA+GGG - chr3.4:37411233-37411252 MS.gene019081:CDS 40.0%
TAAAGGGAGTGGCAATGATT+AGG + chr3.4:37410771-37410790 None:intergenic 40.0%
TACCACGTTTACGATAGGTA+TGG + chr3.4:37410387-37410406 None:intergenic 40.0%
TATTCCTGGAAGTGGTTCTT+CGG - chr3.4:37410738-37410757 MS.gene019081:intron 40.0%
TCCACAATGTGCCAATATTC+GGG + chr3.4:37410246-37410265 None:intergenic 40.0%
TCTTGAGGAAGATACTCAAG+GGG - chr3.4:37411234-37411253 MS.gene019081:CDS 40.0%
TGCAACATTTGGAGACGTAT+TGG - chr3.4:37410189-37410208 MS.gene019081:CDS 40.0%
TGTCTTGAGGAAGATACTCA+AGG - chr3.4:37411232-37411251 MS.gene019081:CDS 40.0%
! CTTGGTTGAGTTGTTTCGTT+AGG - chr3.4:37410588-37410607 MS.gene019081:intron 40.0%
! GGATTAGGTTTGTCACTTGT+GGG - chr3.4:37410493-37410512 MS.gene019081:intron 40.0%
! GTCCTCCGAGATTTTTCTTT+TGG - chr3.4:37410928-37410947 MS.gene019081:intron 40.0%
! TGGATTAGGTTTGTCACTTG+TGG - chr3.4:37410492-37410511 MS.gene019081:intron 40.0%
! TGGTTGAGTTGTTTCGTTAG+GGG - chr3.4:37410590-37410609 MS.gene019081:intron 40.0%
! TGTGGTATAGGCCTTTTAAC+TGG - chr3.4:37410529-37410548 MS.gene019081:intron 40.0%
!! TCTGTAGTTAGTCTTGTCTG+GGG - chr3.4:37410896-37410915 MS.gene019081:intron 40.0%
!!! GGCCAAGTATTTTGATGATG+AGG - chr3.4:37411199-37411218 MS.gene019081:CDS 40.0%
!!! TTCTCTGTGGGAAGTGTTTT+TGG - chr3.4:37410811-37410830 MS.gene019081:intron 40.0%
AAAATCCGCACCCCAAGATT+AGG + chr3.4:37410965-37410984 None:intergenic 45.0%
AAGACGTTTCAGTGCATGTC+CGG - chr3.4:37411114-37411133 MS.gene019081:CDS 45.0%
AAGGTGAAGAGGAAGAGGAA+GGG - chr3.4:37410326-37410345 MS.gene019081:intron 45.0%
AGCGATACCACGTTTACGAT+AGG + chr3.4:37410392-37410411 None:intergenic 45.0%
AGGCGAAACCAATTCTCTGT+GGG - chr3.4:37410799-37410818 MS.gene019081:intron 45.0%
ATAGGGAGGCGATACTAAAG+TGG + chr3.4:37410357-37410376 None:intergenic 45.0%
ATTGCCACTCCCTTTATGAG+TGG - chr3.4:37410775-37410794 MS.gene019081:intron 45.0%
CACCATACCTATCGTAAACG+TGG - chr3.4:37410382-37410401 MS.gene019081:intron 45.0%
TAGCGTATAGGGTGTGGTAT+AGG - chr3.4:37410517-37410536 MS.gene019081:intron 45.0%
TGTTTCCTAATCTTGGGGTG+CGG - chr3.4:37410957-37410976 MS.gene019081:intron 45.0%
TTACACACTTCACGAGGTGA+AGG - chr3.4:37411178-37411197 MS.gene019081:CDS 45.0%
TTGCCACTCCCTTTATGAGT+GGG - chr3.4:37410776-37410795 MS.gene019081:intron 45.0%
TTTCGCCTCCCACTCATAAA+GGG + chr3.4:37410787-37410806 None:intergenic 45.0%
! TGAGTTGTTTCGTTAGGGGT+GGG - chr3.4:37410594-37410613 MS.gene019081:intron 45.0%
! TTGAGTTGTTTCGTTAGGGG+TGG - chr3.4:37410593-37410612 MS.gene019081:intron 45.0%
!! CACTTGTGGGTTAGCGTATA+GGG - chr3.4:37410506-37410525 MS.gene019081:intron 45.0%
!! CTTTTCCATGGTGTTTTGGG+CGG + chr3.4:37410431-37410450 None:intergenic 45.0%
!! TCACTTGTGGGTTAGCGTAT+AGG - chr3.4:37410505-37410524 MS.gene019081:intron 45.0%
AAGAGCAACTCTGCAGCTCT+TGG - chr3.4:37411137-37411156 MS.gene019081:CDS 50.0%
AAGGTCTGTACAACAGCTGC+CGG + chr3.4:37411012-37411031 None:intergenic 50.0%
AGGAGGACGCTATTGTCTTG+AGG - chr3.4:37411219-37411238 MS.gene019081:CDS 50.0%
CGCCCAAAACACCATGGAAA+AGG - chr3.4:37410429-37410448 MS.gene019081:intron 50.0%
CTCGTTACTCGCCCGAATAT+TGG - chr3.4:37410232-37410251 MS.gene019081:CDS 50.0%
CTTCCTCTTCACCTTCAGAG+AGG + chr3.4:37410321-37410340 None:intergenic 50.0%
GAAGGTGAAGAGGAAGAGGA+AGG - chr3.4:37410325-37410344 MS.gene019081:intron 50.0%
GAGGCGAAACCAATTCTCTG+TGG - chr3.4:37410798-37410817 MS.gene019081:intron 50.0%
GCCCGAATATTGGCACATTG+TGG - chr3.4:37410242-37410261 MS.gene019081:CDS 50.0%
GGTATGGTGAACGAATAGGG+AGG + chr3.4:37410371-37410390 None:intergenic 50.0%
GGTTCTCTCTACCTTGAGAC+CGG - chr3.4:37410990-37411009 MS.gene019081:CDS 50.0%
GTTGCTTACCTCCTCTCTGA+AGG - chr3.4:37410307-37410326 MS.gene019081:CDS 50.0%
GTTTCGCCTCCCACTCATAA+AGG + chr3.4:37410788-37410807 None:intergenic 50.0%
TTCACCGAAGAACCACTTCC+AGG + chr3.4:37410745-37410764 None:intergenic 50.0%
TTGTTTCGTTAGGGGTGGGA+AGG - chr3.4:37410598-37410617 MS.gene019081:intron 50.0%
! CTCTGAAGGTGAAGAGGAAG+AGG - chr3.4:37410321-37410340 MS.gene019081:intron 50.0%
! TTGGGGTGCGGATTTTCTCT+AGG - chr3.4:37410969-37410988 MS.gene019081:intron 50.0%
!! GATGGGTTACACACTTCACG+AGG - chr3.4:37411172-37411191 MS.gene019081:CDS 50.0%
CATGTCGCGGACAATTGCAG+CGG - chr3.4:37410277-37410296 MS.gene019081:CDS 55.0%
CCACTCCCTTTATGAGTGGG+AGG - chr3.4:37410779-37410798 MS.gene019081:intron 55.0%
CCTCCCACTCATAAAGGGAG+TGG + chr3.4:37410782-37410801 None:intergenic 55.0%
CCTCTTCACCTTCAGAGAGG+AGG + chr3.4:37410318-37410337 None:intergenic 55.0%
GAGCTGCAGAGTTGCTCTTC+CGG + chr3.4:37411136-37411155 None:intergenic 55.0%
TACAACAGCTGCCGGTCTCA+AGG + chr3.4:37411004-37411023 None:intergenic 55.0%
! CCTCCTCTCTGAAGGTGAAG+AGG - chr3.4:37410315-37410334 MS.gene019081:intron 55.0%
!! GTCTTGTCTGGGGAGTGTTC+TGG - chr3.4:37410906-37410925 MS.gene019081:intron 55.0%
!! GTGGGTTAGCGTATAGGGTG+TGG - chr3.4:37410511-37410530 MS.gene019081:intron 55.0%
!! TTCCATGGTGTTTTGGGCGG+CGG + chr3.4:37410428-37410447 None:intergenic 55.0%
GTGGGAAGGCAGAGTAGGCT+AGG - chr3.4:37410612-37410631 MS.gene019081:intron 60.0%
TAGGGGTGGGAAGGCAGAGT+AGG - chr3.4:37410607-37410626 MS.gene019081:intron 60.0%
CACCGCCGCCCAAAACACCA+TGG - chr3.4:37410423-37410442 MS.gene019081:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 37410160 37411274 37410160 ID=MS.gene019081
chr3.4 mRNA 37410160 37411274 37410160 ID=MS.gene019081.t1;Parent=MS.gene019081
chr3.4 exon 37410984 37411274 37410984 ID=MS.gene019081.t1.exon1;Parent=MS.gene019081.t1
chr3.4 CDS 37410984 37411274 37410984 ID=cds.MS.gene019081.t1;Parent=MS.gene019081.t1
chr3.4 exon 37410160 37410330 37410160 ID=MS.gene019081.t1.exon2;Parent=MS.gene019081.t1
chr3.4 CDS 37410160 37410330 37410160 ID=cds.MS.gene019081.t1;Parent=MS.gene019081.t1
Gene Sequence

>MS.gene019081

ATGTCGTCAAAGAAGGTGTGCATAAGAGTTGCAACATTTGGAGACGTATTGGATTGGAACAGTAACAACAATCTCGTTACTCGCCCGAATATTGGCACATTGTGGAAGAAGCTTATTCATGTCGCGGACAATTGCAGCGGCTGTATTGTTGCTTACCTCCTCTCTGAAGGTGAAGAGGAAGAGGAAGGGAATGACCACTTTAGTATCGCCTCCCTATTCGTTCACCATACCTATCGTAAACGTGGTATCGCTACTGATCTTATCACCGCCGCCCAAAACACCATGGAAAAGGTTAGGTTTGTTTCTTTATATTCTGTTATAATTTTGTTTTTTGGATTAGGTTTGTCACTTGTGGGTTAGCGTATAGGGTGTGGTATAGGCCTTTTAACTGGTTATGTATGGAGTTTGTTTAATGTTTAGGTATGAAGCTTGGTTGAGTTGTTTCGTTAGGGGTGGGAAGGCAGAGTAGGCTAGGTTTTTTGTTAATCTAGCACGCCACTGTGTGATCTATCTGAAAACCCGTGATGATTTTCTGTTTTCTGGTAGAATCTGAGTTTCACTATGCTAGGATTATCTTCTATTCCTGGAAGTGGTTCTTCGGTGAATTCCCTAATCATTGCCACTCCCTTTATGAGTGGGAGGCGAAACCAATTCTCTGTGGGAAGTGTTTTTGGCTTCTTTTCTTCTTTCTCTTTCTTCTTGTTAATGATTTTGGTGGTTGGTGTTTGTTGTTTTTTCTGTAGTTAGTCTTGTCTGGGGAGTGTTCTGGTCCTCCGAGATTTTTCTTTTGGTTTTCTTGTTTCCTAATCTTGGGGTGCGGATTTTCTCTAGGTTCTCTCTACCTTGAGACCGGCAGCTGTTGTACAGACCTTTTTTGATGTTTTTGGTTGTCAATACCTATTTTTTCCATTAAAAGTCTGTTACAATGTGTAATTTGACTGAAGGTGGGTGCTAAAGACGTTTCAGTGCATGTCCGGAAGAGCAACTCTGCAGCTCTTGGTTTGTTTCAAAAGATGGGTTACACACTTCACGAGGTGAAGGCCAAGTATTTTGATGATGAGGAGGACGCTATTGTCTTGAGGAAGATACTCAAGGGGAAGCATGTTGCCACCTAA

Protein sequence

>MS.gene019081.t1

MSSKKVCIRVATFGDVLDWNSNNNLVTRPNIGTLWKKLIHVADNCSGCIVAYLLSEGEEEEEGNDHFSIASLFVHHTYRKRGIATDLITAAQNTMEKVGAKDVSVHVRKSNSAALGLFQKMGYTLHEVKAKYFDDEEDAIVLRKILKGKHVAT