AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023327

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023327.t1 MTR_4g112670 84.132 334 47 4 1 328 1 334 0.0 555
MS.gene023327.t1 MTR_4g112650 77.844 334 68 3 2 329 1 334 0.0 524
MS.gene023327.t1 MTR_4g112630 73.214 336 73 4 2 330 1 326 1.16e-173 484
MS.gene023327.t1 MTR_4g112620 69.162 334 64 5 1 329 1 300 2.72e-159 447
MS.gene023327.t1 MTR_2g058450 54.397 307 137 2 25 328 28 334 9.68e-109 320
MS.gene023327.t1 MTR_0003s0530 81.667 180 29 2 145 320 2 181 5.32e-99 291
MS.gene023327.t1 MTR_7g490330 38.298 329 183 4 4 329 13 324 1.20e-75 235
MS.gene023327.t1 MTR_7g490350 38.245 319 168 3 36 328 52 367 1.67e-74 234
MS.gene023327.t1 MTR_2g058410 53.017 232 106 1 101 329 1 232 3.43e-73 226
MS.gene023327.t1 MTR_7g090300 38.629 321 166 5 36 329 52 368 2.31e-71 226
MS.gene023327.t1 MTR_5g091980 39.645 338 172 8 2 329 7 322 5.93e-70 221
MS.gene023327.t1 MTR_4g050190 37.758 339 172 10 24 328 56 389 2.89e-68 219
MS.gene023327.t1 MTR_2g087780 36.686 338 177 9 24 328 60 393 6.10e-68 218
MS.gene023327.t1 MTR_7g090320 37.110 353 181 9 9 325 35 382 1.36e-66 214
MS.gene023327.t1 MTR_7g090310 37.812 320 164 6 36 320 57 376 1.09e-62 204
MS.gene023327.t1 MTR_2g099030 34.431 334 186 7 25 326 70 402 3.10e-60 198
MS.gene023327.t1 MTR_4g112840 80.000 100 18 1 171 268 56 155 1.07e-50 166
MS.gene023327.t1 MTR_5g077620 86.905 84 11 0 4 87 1 84 6.51e-46 153
MS.gene023327.t1 MTR_5g077620 90.698 43 4 0 274 316 76 118 4.32e-19 82.8
MS.gene023327.t1 MTR_8g074210 33.463 257 139 6 21 269 56 288 3.00e-34 129
MS.gene023327.t1 MTR_8g074270 34.043 235 129 6 37 269 78 288 7.88e-32 122
MS.gene023327.t1 MTR_8g074170 34.061 229 125 6 37 263 78 282 7.23e-31 120
MS.gene023327.t1 MTR_2g042430 31.878 229 127 9 38 263 77 279 3.51e-23 99.0
MS.gene023327.t1 MTR_2g042420 31.441 229 128 9 38 263 58 260 2.78e-22 96.3
MS.gene023327.t1 MTR_2g042370 35.220 159 98 4 108 263 112 268 1.02e-21 94.7
MS.gene023327.t1 MTR_2g058430 54.118 85 39 0 90 174 50 134 2.71e-21 92.0
MS.gene023327.t1 MTR_8g074320 37.795 127 72 4 98 219 95 219 2.82e-17 79.7
MS.gene023327.t1 MTR_1g075865 37.963 108 64 2 148 252 4 111 4.56e-17 77.0
MS.gene023327.t1 MTR_5g093640 45.918 98 7 2 233 330 52 103 1.27e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023327.t1 AT1G74020 42.345 307 163 6 27 328 28 325 7.05e-74 231
MS.gene023327.t1 AT3G57030 39.875 321 162 5 36 328 55 372 2.62e-73 231
MS.gene023327.t1 AT1G74010 42.623 305 162 7 27 327 27 322 3.43e-72 227
MS.gene023327.t1 AT1G74000 40.391 307 169 6 27 328 30 327 2.42e-69 219
MS.gene023327.t1 AT3G57010 41.393 244 135 3 24 265 41 278 1.92e-67 216
MS.gene023327.t1 AT1G08470 36.747 332 175 8 28 328 60 387 3.37e-67 216
MS.gene023327.t1 AT5G22020 36.310 336 178 9 25 328 62 393 1.28e-66 214
MS.gene023327.t1 AT5G22020 36.310 336 178 9 25 328 62 393 1.28e-66 214
MS.gene023327.t1 AT5G22020 36.310 336 178 9 25 328 62 393 1.28e-66 214
MS.gene023327.t1 AT3G57020 38.934 244 141 2 24 265 40 277 8.08e-62 201
MS.gene023327.t1 AT2G41290 37.003 327 168 9 37 328 51 374 4.80e-60 197
MS.gene023327.t1 AT3G59530 38.554 249 145 4 28 269 75 322 6.95e-57 189
MS.gene023327.t1 AT3G59530 38.554 249 145 4 28 269 75 322 6.95e-57 189
MS.gene023327.t1 AT3G59530 38.554 249 145 4 28 269 75 322 6.95e-57 189
MS.gene023327.t1 AT3G57020 36.885 244 132 2 24 265 40 263 1.02e-56 187
MS.gene023327.t1 AT2G41300 33.442 308 171 6 42 321 83 384 8.54e-55 184
MS.gene023327.t1 AT3G51440 27.698 278 156 9 35 304 67 307 1.30e-25 105
MS.gene023327.t1 AT3G51430 30.303 231 136 7 38 265 70 278 3.09e-24 102
MS.gene023327.t1 AT3G51430 29.870 231 137 7 38 265 70 278 4.71e-24 101
MS.gene023327.t1 AT3G51420 27.476 313 184 12 35 326 67 357 3.46e-23 99.0
MS.gene023327.t1 AT3G51450 29.487 234 134 8 38 265 70 278 1.27e-22 97.4

Find 80 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATTCAAGAGGCCAATTT+TGG 0.142569 4.2:+15498505 MS.gene023327:CDS
ATACAAAGAGTTTGGCTTAA+AGG 0.191016 4.2:+15498420 MS.gene023327:CDS
ATTGGTAACCATGTTGAATA+TGG 0.244460 4.2:+15497300 None:intergenic
GCAAAATGTGGAAGACCTTT+AGG 0.259656 4.2:+15497953 MS.gene023327:CDS
GAATTTCTCTTGATGTTATC+TGG 0.262648 4.2:-15498489 None:intergenic
TGCTTACTTGAGCTGGAAAT+TGG 0.273371 4.2:-15498164 None:intergenic
ATTTCAGTGAATAGTTATTT+AGG 0.284952 4.2:+15498528 MS.gene023327:CDS
TATTCACTGAAATCCAAAAT+TGG 0.293830 4.2:-15498518 None:intergenic
ATCAGCAAACTTAGTAGAAT+TGG 0.304645 4.2:-15498089 None:intergenic
CGGGCAAAATCGTCCGTCTT+GGG 0.319086 4.2:-15498563 None:intergenic
AGCTTGGATCATATCTTAAT+AGG 0.319761 4.2:-15498293 None:intergenic
CAAACAGAATACAAAGAGTT+TGG 0.323375 4.2:+15498412 MS.gene023327:CDS
CAAAATGTGGAAGACCTTTA+GGG 0.331794 4.2:+15497954 MS.gene023327:CDS
TACTAAGTTTGCTGATGGTT+TGG 0.332839 4.2:+15498096 MS.gene023327:CDS
GTATCTCTTGGTGCTGAGTA+TGG 0.338967 4.2:+15498627 MS.gene023327:CDS
GATATATTTATTTACCTGTT+TGG 0.346046 4.2:-15497538 None:intergenic
TACGTAGCTGATGCTTATTA+TGG 0.348144 4.2:+15498007 MS.gene023327:CDS
AATTATCTCCGCTCAGTGTC+TGG 0.353838 4.2:-15498263 None:intergenic
GGCAATCTTGCCGTGCCATC+TGG 0.359780 4.2:+15498339 MS.gene023327:CDS
GAATTATTCATGTTACTTGC+TGG 0.369585 4.2:+15498462 MS.gene023327:CDS
CTCAGTCACTCTGATTCCTC+CGG 0.409015 4.2:-15498583 None:intergenic
TGCTTATTATGGCCTTGTTA+AGG 0.409890 4.2:+15498018 MS.gene023327:CDS
TGAGTTACATTTGCTCCATC+AGG 0.418940 4.2:-15498046 None:intergenic
CCGGGCAAAATCGTCCGTCT+TGG 0.422570 4.2:-15498564 None:intergenic
AATTCTGATGAATTTGCTCT+TGG 0.423029 4.2:-15498444 None:intergenic
AGTGAGATTCAAGAGTATAA+TGG 0.429524 4.2:+15498663 MS.gene023327:CDS
TGCCTAGCAACACCGTCGTT+TGG 0.431318 4.2:-15498320 None:intergenic
ATCGTCCGTCTTGGGGATCC+TGG 0.434461 4.2:-15498555 None:intergenic
ATGCTTTCACTTCGCCAAAC+AGG 0.434792 4.2:+15497524 MS.gene023327:CDS
GAAACTATGCTTACTTGAGC+TGG 0.440498 4.2:-15498171 None:intergenic
AAATATATTGGTCCAGATGA+AGG 0.448616 4.2:+15497490 MS.gene023327:CDS
TATTCCTTGAAACCTTCATC+TGG 0.461685 4.2:-15497502 None:intergenic
CTGGAAATTGGTGCTAGCTT+CGG 0.463996 4.2:-15498152 None:intergenic
CTTGTTAAGGTTAGTCCTGA+TGG 0.465547 4.2:+15498031 MS.gene023327:CDS
TCACTTGCATTTGATAGAAA+TGG 0.471351 4.2:+15497427 MS.gene023327:CDS
GCAAATGTAACTCAATTAGT+TGG 0.471990 4.2:+15498055 MS.gene023327:CDS
TGGTCCAGATGAAGGTTTCA+AGG 0.472200 4.2:+15497498 MS.gene023327:CDS
CCTTATGTTGGCTCCTCTGA+TGG 0.473712 4.2:+15497457 MS.gene023327:CDS
ACCGTCGTTTGGTTTGAGCT+TGG 0.476304 4.2:-15498309 None:intergenic
TACTCTTGAATCTCACTAGC+TGG 0.484772 4.2:-15498657 None:intergenic
GGAATCAGAGTGACTGAGAA+TGG 0.488784 4.2:+15498588 MS.gene023327:CDS
TTAGTAGAATTGGCTTGTGC+AGG 0.492686 4.2:-15498079 None:intergenic
ACACTGAGCGGAGATAATTC+AGG 0.493415 4.2:+15498267 MS.gene023327:CDS
TGCTAGCATAAGAAGCAAGC+AGG 0.502038 4.2:-15498704 None:intergenic
TATAATGGAACGCTCTATAG+TGG 0.504379 4.2:+15498678 MS.gene023327:CDS
CCATCAGAGGAGCCAACATA+AGG 0.508307 4.2:-15497457 None:intergenic
TTGGATGTAGATTCCGACAC+TGG 0.519237 4.2:+15498115 MS.gene023327:CDS
CTATCAAATGCAAGTGACTC+AGG 0.521823 4.2:-15497421 None:intergenic
GGGCAAAATCGTCCGTCTTG+GGG 0.530545 4.2:-15498562 None:intergenic
CTTGCATTTGATAGAAATGG+TGG 0.539336 4.2:+15497430 MS.gene023327:CDS
AAAATAAACGATTCCAGTGT+CGG 0.540943 4.2:-15498128 None:intergenic
AGTGACTCAGGACCCGTCAC+TGG 0.541604 4.2:-15497409 None:intergenic
TCAGTCACTCTGATTCCTCC+GGG 0.543329 4.2:-15498582 None:intergenic
AACCAAACGACGGTGTTGCT+AGG 0.548086 4.2:+15498318 MS.gene023327:CDS
GGTGTGGCAGTAAGCAAAGA+TGG 0.552392 4.2:+15498360 MS.gene023327:CDS
TTGCTTACTGCCACACCAGA+TGG 0.555964 4.2:-15498354 None:intergenic
TACTGCCACACCAGATGGCA+CGG 0.561460 4.2:-15498349 None:intergenic
TGATTGAAACTCAACCCTAA+AGG 0.563318 4.2:-15497968 None:intergenic
TTGAGTTTCAATCATCAGAC+GGG 0.566766 4.2:+15497977 MS.gene023327:CDS
AATTCTACTAAGTTTGCTGA+TGG 0.568034 4.2:+15498091 MS.gene023327:CDS
TCAGCTACCATCACCAGTGA+CGG 0.576414 4.2:+15497396 MS.gene023327:CDS
TCAGGACCCGTCACTGGTGA+TGG 0.579014 4.2:-15497403 None:intergenic
GCTAGCATAAGAAGCAAGCA+GGG 0.580108 4.2:-15498703 None:intergenic
GTCCGTCTTGGGGATCCTGG+TGG 0.581851 4.2:-15498552 None:intergenic
AGGAACAAGACAATATGTGA+TGG 0.582363 4.2:+15497905 MS.gene023327:intron
TCTTGCCGTGCCATCTGGTG+TGG 0.588262 4.2:+15498344 MS.gene023327:CDS
GTTGAGTTTCAATCATCAGA+CGG 0.594215 4.2:+15497976 MS.gene023327:CDS
GGAACAAGACAATATGTGAT+GGG 0.595406 4.2:+15497906 MS.gene023327:intron
CAGCTACCATCACCAGTGAC+GGG 0.598096 4.2:+15497397 MS.gene023327:CDS
AATAGTTATTTAGGTCCACC+AGG 0.607064 4.2:+15498537 MS.gene023327:CDS
TCAGCAGTCCAAGCAAAATG+TGG 0.607995 4.2:+15497941 MS.gene023327:CDS
AATGGTGGAGGACCTTATGT+TGG 0.609545 4.2:+15497445 MS.gene023327:CDS
GCATTTGATAGAAATGGTGG+AGG 0.613633 4.2:+15497433 MS.gene023327:CDS
AACATCAAGAGAAATTCAAG+AGG 0.624735 4.2:+15498495 MS.gene023327:CDS
CATCAGGACTAACCTTAACA+AGG 0.625058 4.2:-15498030 None:intergenic
GTCCACCAGGATCCCCAAGA+CGG 0.653542 4.2:+15498550 MS.gene023327:CDS
AAACATTGCCATATTCAACA+TGG 0.672591 4.2:-15497308 None:intergenic
AGGGATTTCCAGACACTGAG+CGG 0.682326 4.2:+15498255 MS.gene023327:intron
AGTAAGTAAAATACTCTAGG+GGG 0.687710 4.2:-15498733 None:intergenic
TCCAAGCTCAAACCAAACGA+CGG 0.707803 4.2:+15498308 MS.gene023327:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAACTTTTTACAAAATAAA+CGG - chr4.2:15497617-15497636 None:intergenic 10.0%
!!! AATGAATTCCTTTTTTATTT+TGG + chr4.2:15497879-15497898 MS.gene023327:intron 15.0%
!!! GGAAGAATTTTTAAATATAT+TGG + chr4.2:15497478-15497497 MS.gene023327:CDS 15.0%
!!! TTTTTTTTTTTTTGTGTGAT+CGG + chr4.2:15497659-15497678 MS.gene023327:intron 15.0%
!! ATGTTTATATAGAAAACACT+GGG + chr4.2:15497784-15497803 MS.gene023327:intron 20.0%
!! ATTTCAGTGAATAGTTATTT+AGG + chr4.2:15498528-15498547 MS.gene023327:CDS 20.0%
!! GATATATTTATTTACCTGTT+TGG - chr4.2:15497541-15497560 None:intergenic 20.0%
!!! TTTTTTTTTTTTGTGTGATC+GGG + chr4.2:15497660-15497679 MS.gene023327:intron 20.0%
! AAACATGAAAAGAAGATGAT+AGG - chr4.2:15497770-15497789 None:intergenic 25.0%
! CATGTTTATATAGAAAACAC+TGG + chr4.2:15497783-15497802 MS.gene023327:intron 25.0%
! TATTCACTGAAATCCAAAAT+TGG - chr4.2:15498521-15498540 None:intergenic 25.0%
! TTAGTATTGTAATGCAGAAT+TGG - chr4.2:15498210-15498229 None:intergenic 25.0%
! TTCCTGAACCAAAATAAAAA+AGG - chr4.2:15497890-15497909 None:intergenic 25.0%
! TTTAAAAATTCTTCCATCAG+AGG - chr4.2:15497473-15497492 None:intergenic 25.0%
!! ATTTTACAGATTGTATCTCT+TGG + chr4.2:15498615-15498634 MS.gene023327:CDS 25.0%
!!! TTCCTTTTTTATTTTGGTTC+AGG + chr4.2:15497885-15497904 MS.gene023327:intron 25.0%
AAAATAAACGATTCCAGTGT+CGG - chr4.2:15498131-15498150 None:intergenic 30.0%
AAATATATTGGTCCAGATGA+AGG + chr4.2:15497490-15497509 MS.gene023327:CDS 30.0%
AACATCAAGAGAAATTCAAG+AGG + chr4.2:15498495-15498514 MS.gene023327:CDS 30.0%
AGCTTGGATCATATCTTAAT+AGG - chr4.2:15498296-15498315 None:intergenic 30.0%
ATCAGCAAACTTAGTAGAAT+TGG - chr4.2:15498092-15498111 None:intergenic 30.0%
ATGATAGGCTAAATTTCTTG+AGG - chr4.2:15497755-15497774 None:intergenic 30.0%
CAAACAGAATACAAAGAGTT+TGG + chr4.2:15498412-15498431 MS.gene023327:CDS 30.0%
GAATTTCTCTTGATGTTATC+TGG - chr4.2:15498492-15498511 None:intergenic 30.0%
GCAAATGTAACTCAATTAGT+TGG + chr4.2:15498055-15498074 MS.gene023327:CDS 30.0%
TCACTTGCATTTGATAGAAA+TGG + chr4.2:15497427-15497446 MS.gene023327:CDS 30.0%
TGAATCTAGCTAGCTATAAT+AGG - chr4.2:15497572-15497591 None:intergenic 30.0%
TGATAGGCTAAATTTCTTGA+GGG - chr4.2:15497754-15497773 None:intergenic 30.0%
! AATTCTGATGAATTTGCTCT+TGG - chr4.2:15498447-15498466 None:intergenic 30.0%
! AGTGAGATTCAAGAGTATAA+TGG + chr4.2:15498663-15498682 MS.gene023327:CDS 30.0%
! ATACAAAGAGTTTGGCTTAA+AGG + chr4.2:15498420-15498439 MS.gene023327:CDS 30.0%
! ATGTCACTTGGTTAATTGTA+GGG + chr4.2:15498236-15498255 MS.gene023327:intron 30.0%
! GAATTATTCATGTTACTTGC+TGG + chr4.2:15498462-15498481 MS.gene023327:CDS 30.0%
!! AATTCTACTAAGTTTGCTGA+TGG + chr4.2:15498091-15498110 MS.gene023327:CDS 30.0%
AATAGTTATTTAGGTCCACC+AGG + chr4.2:15498537-15498556 MS.gene023327:CDS 35.0%
AGGAACAAGACAATATGTGA+TGG + chr4.2:15497905-15497924 MS.gene023327:intron 35.0%
CAAAATGTGGAAGACCTTTA+GGG + chr4.2:15497954-15497973 MS.gene023327:CDS 35.0%
CTTGCATTTGATAGAAATGG+TGG + chr4.2:15497430-15497449 MS.gene023327:CDS 35.0%
GGAACAAGACAATATGTGAT+GGG + chr4.2:15497906-15497925 MS.gene023327:intron 35.0%
GTTGAGTTTCAATCATCAGA+CGG + chr4.2:15497976-15497995 MS.gene023327:CDS 35.0%
TACGTAGCTGATGCTTATTA+TGG + chr4.2:15498007-15498026 MS.gene023327:CDS 35.0%
TACTAAATGTGCATGTCACT+TGG + chr4.2:15498224-15498243 MS.gene023327:intron 35.0%
TATTCCTTGAAACCTTCATC+TGG - chr4.2:15497505-15497524 None:intergenic 35.0%
TGATTGAAACTCAACCCTAA+AGG - chr4.2:15497971-15497990 None:intergenic 35.0%
TTGAGTTTCAATCATCAGAC+GGG + chr4.2:15497977-15497996 MS.gene023327:CDS 35.0%
! CATGTCACTTGGTTAATTGT+AGG + chr4.2:15498235-15498254 MS.gene023327:intron 35.0%
! GAAATTCAAGAGGCCAATTT+TGG + chr4.2:15498505-15498524 MS.gene023327:CDS 35.0%
! TATAATGGAACGCTCTATAG+TGG + chr4.2:15498678-15498697 MS.gene023327:CDS 35.0%
!! TACTAAGTTTGCTGATGGTT+TGG + chr4.2:15498096-15498115 MS.gene023327:CDS 35.0%
!! TGCTTATTATGGCCTTGTTA+AGG + chr4.2:15498018-15498037 MS.gene023327:CDS 35.0%
CATCAGGACTAACCTTAACA+AGG - chr4.2:15498033-15498052 None:intergenic 40.0%
CTATCAAATGCAAGTGACTC+AGG - chr4.2:15497424-15497443 None:intergenic 40.0%
GAAACTATGCTTACTTGAGC+TGG - chr4.2:15498174-15498193 None:intergenic 40.0%
GCAAAATGTGGAAGACCTTT+AGG + chr4.2:15497953-15497972 MS.gene023327:CDS 40.0%
GCATTTGATAGAAATGGTGG+AGG + chr4.2:15497433-15497452 MS.gene023327:CDS 40.0%
TACTCTTGAATCTCACTAGC+TGG - chr4.2:15498660-15498679 None:intergenic 40.0%
TGAGTTACATTTGCTCCATC+AGG - chr4.2:15498049-15498068 None:intergenic 40.0%
TGCTTACTTGAGCTGGAAAT+TGG - chr4.2:15498167-15498186 None:intergenic 40.0%
! AAGGTCTTCCACATTTTGCT+TGG - chr4.2:15497952-15497971 None:intergenic 40.0%
! CTTGTTAAGGTTAGTCCTGA+TGG + chr4.2:15498031-15498050 MS.gene023327:CDS 40.0%
!! TTAGTAGAATTGGCTTGTGC+AGG - chr4.2:15498082-15498101 None:intergenic 40.0%
AATTATCTCCGCTCAGTGTC+TGG - chr4.2:15498266-15498285 None:intergenic 45.0%
ACACTGAGCGGAGATAATTC+AGG + chr4.2:15498267-15498286 MS.gene023327:CDS 45.0%
ATGCTTTCACTTCGCCAAAC+AGG + chr4.2:15497524-15497543 MS.gene023327:CDS 45.0%
GCTAGCATAAGAAGCAAGCA+GGG - chr4.2:15498706-15498725 None:intergenic 45.0%
GGAATCAGAGTGACTGAGAA+TGG + chr4.2:15498588-15498607 MS.gene023327:CDS 45.0%
TCAGCAGTCCAAGCAAAATG+TGG + chr4.2:15497941-15497960 MS.gene023327:CDS 45.0%
TCCAAGCTCAAACCAAACGA+CGG + chr4.2:15498308-15498327 MS.gene023327:CDS 45.0%
TGCTAGCATAAGAAGCAAGC+AGG - chr4.2:15498707-15498726 None:intergenic 45.0%
TGGTCCAGATGAAGGTTTCA+AGG + chr4.2:15497498-15497517 MS.gene023327:CDS 45.0%
TTGGATGTAGATTCCGACAC+TGG + chr4.2:15498115-15498134 MS.gene023327:CDS 45.0%
! AATGGTGGAGGACCTTATGT+TGG + chr4.2:15497445-15497464 MS.gene023327:CDS 45.0%
!! CTGGAAATTGGTGCTAGCTT+CGG - chr4.2:15498155-15498174 None:intergenic 45.0%
!! GTATCTCTTGGTGCTGAGTA+TGG + chr4.2:15498627-15498646 MS.gene023327:CDS 45.0%
AGGGATTTCCAGACACTGAG+CGG + chr4.2:15498255-15498274 MS.gene023327:intron 50.0%
CCATCAGAGGAGCCAACATA+AGG - chr4.2:15497460-15497479 None:intergenic 50.0%
CTCAGTCACTCTGATTCCTC+CGG - chr4.2:15498586-15498605 None:intergenic 50.0%
CTGAGGCTATTCCATCGAGT+CGG + chr4.2:15497702-15497721 MS.gene023327:intron 50.0%
GGTGTGGCAGTAAGCAAAGA+TGG + chr4.2:15498360-15498379 MS.gene023327:CDS 50.0%
TCAGCTACCATCACCAGTGA+CGG + chr4.2:15497396-15497415 MS.gene023327:CDS 50.0%
TCAGTCACTCTGATTCCTCC+GGG - chr4.2:15498585-15498604 None:intergenic 50.0%
TGAGGCTATTCCATCGAGTC+GGG + chr4.2:15497703-15497722 MS.gene023327:intron 50.0%
TTGCTTACTGCCACACCAGA+TGG - chr4.2:15498357-15498376 None:intergenic 50.0%
!! AACCAAACGACGGTGTTGCT+AGG + chr4.2:15498318-15498337 MS.gene023327:CDS 50.0%
!! ACCGTCGTTTGGTTTGAGCT+TGG - chr4.2:15498312-15498331 None:intergenic 50.0%
!! CCTTATGTTGGCTCCTCTGA+TGG + chr4.2:15497457-15497476 MS.gene023327:CDS 50.0%
ATCGAGTCGGGTGGGATCTA+TGG + chr4.2:15497715-15497734 MS.gene023327:intron 55.0%
CAGCTACCATCACCAGTGAC+GGG + chr4.2:15497397-15497416 MS.gene023327:CDS 55.0%
CGGGCAAAATCGTCCGTCTT+GGG - chr4.2:15498566-15498585 None:intergenic 55.0%
GCTATTCCATCGAGTCGGGT+GGG + chr4.2:15497707-15497726 MS.gene023327:intron 55.0%
GGGCAAAATCGTCCGTCTTG+GGG - chr4.2:15498565-15498584 None:intergenic 55.0%
TACTGCCACACCAGATGGCA+CGG - chr4.2:15498352-15498371 None:intergenic 55.0%
TCGAGTCGGGTGGGATCTAT+GGG + chr4.2:15497716-15497735 MS.gene023327:intron 55.0%
TGCCTAGCAACACCGTCGTT+TGG - chr4.2:15498323-15498342 None:intergenic 55.0%
! ATAGATCCCACCCGACTCGA+TGG - chr4.2:15497716-15497735 None:intergenic 55.0%
! CCAAGACGGACGATTTTGCC+CGG + chr4.2:15498564-15498583 MS.gene023327:CDS 55.0%
AGTGACTCAGGACCCGTCAC+TGG - chr4.2:15497412-15497431 None:intergenic 60.0%
ATCGTCCGTCTTGGGGATCC+TGG - chr4.2:15498558-15498577 None:intergenic 60.0%
CCGGGCAAAATCGTCCGTCT+TGG - chr4.2:15498567-15498586 None:intergenic 60.0%
GGCAATCTTGCCGTGCCATC+TGG + chr4.2:15498339-15498358 MS.gene023327:CDS 60.0%
GGCTATTCCATCGAGTCGGG+TGG + chr4.2:15497706-15497725 MS.gene023327:intron 60.0%
GTCCACCAGGATCCCCAAGA+CGG + chr4.2:15498550-15498569 MS.gene023327:CDS 60.0%
TCAGGACCCGTCACTGGTGA+TGG - chr4.2:15497406-15497425 None:intergenic 60.0%
TCTTGCCGTGCCATCTGGTG+TGG + chr4.2:15498344-15498363 MS.gene023327:CDS 60.0%
! AGACGGACGATTTTGCCCGG+AGG + chr4.2:15498567-15498586 MS.gene023327:CDS 60.0%
GTCCGTCTTGGGGATCCTGG+TGG - chr4.2:15498555-15498574 None:intergenic 65.0%
TCCTCTGCGCGCGATTGCTG+AGG + chr4.2:15497685-15497704 MS.gene023327:intron 65.0%
GCCTCAGCAATCGCGCGCAG+AGG - chr4.2:15497689-15497708 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 15497310 15498740 15497310 ID=MS.gene023327
chr4.2 mRNA 15497310 15498740 15497310 ID=MS.gene023327.t1;Parent=MS.gene023327
chr4.2 exon 15497310 15497545 15497310 ID=MS.gene023327.t1.exon1;Parent=MS.gene023327.t1
chr4.2 CDS 15497310 15497545 15497310 ID=cds.MS.gene023327.t1;Parent=MS.gene023327.t1
chr4.2 exon 15497907 15498179 15497907 ID=MS.gene023327.t1.exon2;Parent=MS.gene023327.t1
chr4.2 CDS 15497907 15498179 15497907 ID=cds.MS.gene023327.t1;Parent=MS.gene023327.t1
chr4.2 exon 15498257 15498740 15498257 ID=MS.gene023327.t1.exon3;Parent=MS.gene023327.t1
chr4.2 CDS 15498257 15498740 15498257 ID=cds.MS.gene023327.t1;Parent=MS.gene023327.t1
Gene Sequence

>MS.gene023327

ATGTTGAATATGGCAATGTTTGTCACTACAACTCTTGTTATCTTCATGTTATGTTCTCAATCAGTAGCTATATTACAGAAGAAGCTTCAGCTACCATCACCAGTGACGGGTCCTGAGTCACTTGCATTTGATAGAAATGGTGGAGGACCTTATGTTGGCTCCTCTGATGGAAGAATTTTTAAATATATTGGTCCAGATGAAGGTTTCAAGGAATATGCTTTCACTTCGCCAAACAGGTAAATAAATATATCAATCATCACCTATTATAGCTAGCTAGATTCAAAAACTACTACGAGTTAAGTGACCGTTTATTTTGTAAAAAGTTTTATTTTCAATTTTTACTATCATCTTTTTTTTTTTTTGTGTGATCGGGTATCCTCTGCGCGCGATTGCTGAGGCTATTCCATCGAGTCGGGTGGGATCTATGGGAGACAAAGTTTTCCCTCAAGAAATTTAGCCTATCATCTTCTTTTCATGTTTATATAGAAAACACTGGGAAAAAAAAAAACGTTTTTCTTATTTTTTTGTGTGAATAAACAATGTGACAAATTTATAAATGAAAGTTAGTTAATGAATTCCTTTTTTATTTTGGTTCAGGAACAAGACAATATGTGATGGGATTGCAGACTTCTCAGCAGTCCAAGCAAAATGTGGAAGACCTTTAGGGTTGAGTTTCAATCATCAGACGGGCGATTTATACGTAGCTGATGCTTATTATGGCCTTGTTAAGGTTAGTCCTGATGGAGCAAATGTAACTCAATTAGTTGGACCTGCACAAGCCAATTCTACTAAGTTTGCTGATGGTTTGGATGTAGATTCCGACACTGGAATCGTTTATTTTACCGAAGCTAGCACCAATTTCCAGCTCAAGTAAGCATAGTTTCTTGCAGCTAAATGCCAATTCTGCATTACAATACTAAATGTGCATGTCACTTGGTTAATTGTAGGGATTTCCAGACACTGAGCGGAGATAATTCAGGAAGCCTATTAAGATATGATCCAAGCTCAAACCAAACGACGGTGTTGCTAGGCAATCTTGCCGTGCCATCTGGTGTGGCAGTAAGCAAAGATGGCTCATTTGTACTTGTTAGTGAATATCTATCAAACAGAATACAAAGAGTTTGGCTTAAAGGACCAAGAGCAAATTCATCAGAATTATTCATGTTACTTGCTGGAAGACCAGATAACATCAAGAGAAATTCAAGAGGCCAATTTTGGATTTCAGTGAATAGTTATTTAGGTCCACCAGGATCCCCAAGACGGACGATTTTGCCCGGAGGAATCAGAGTGACTGAGAATGGTTTAATTTTACAGATTGTATCTCTTGGTGCTGAGTATGGTACTCAACCAGCTAGTGAGATTCAAGAGTATAATGGAACGCTCTATAGTGGCTCCCTGCTTGCTTCTTATGCTAGCATTTTTACCCCCTAG
Protein sequence

>MS.gene023327.t1

MLNMAMFVTTTLVIFMLCSQSVAILQKKLQLPSPVTGPESLAFDRNGGGPYVGSSDGRIFKYIGPDEGFKEYAFTSPNRNKTICDGIADFSAVQAKCGRPLGLSFNHQTGDLYVADAYYGLVKVSPDGANVTQLVGPAQANSTKFADGLDVDSDTGIVYFTEASTNFQLKDFQTLSGDNSGSLLRYDPSSNQTTVLLGNLAVPSGVAVSKDGSFVLVSEYLSNRIQRVWLKGPRANSSELFMLLAGRPDNIKRNSRGQFWISVNSYLGPPGSPRRTILPGGIRVTENGLILQIVSLGAEYGTQPASEIQEYNGTLYSGSLLASYASIFTP