AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83238

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83238.t1 MTR_2g058450 97.619 336 8 0 8 343 1 336 0.0 667
MS.gene83238.t1 MTR_2g058410 79.310 232 46 2 111 341 1 231 2.41e-118 341
MS.gene83238.t1 MTR_4g112670 53.392 339 151 4 5 341 1 334 3.62e-118 345
MS.gene83238.t1 MTR_4g112650 52.994 334 152 3 8 339 1 331 2.50e-114 335
MS.gene83238.t1 MTR_4g112630 51.632 337 147 4 8 341 1 324 1.01e-107 318
MS.gene83238.t1 MTR_4g112620 46.154 338 142 5 5 341 1 299 1.65e-92 278
MS.gene83238.t1 MTR_7g490330 43.322 307 154 6 38 343 38 325 8.05e-74 231
MS.gene83238.t1 MTR_7g490350 41.615 322 158 6 47 343 53 369 2.98e-72 229
MS.gene83238.t1 MTR_7g090300 42.236 322 156 7 47 343 53 369 2.23e-69 221
MS.gene83238.t1 MTR_7g090320 38.125 320 165 4 47 333 58 377 2.25e-69 222
MS.gene83238.t1 MTR_5g091980 42.997 307 154 4 37 343 38 323 1.08e-68 218
MS.gene83238.t1 MTR_7g090310 37.618 319 167 4 47 333 58 376 1.90e-68 219
MS.gene83238.t1 MTR_4g050190 37.879 330 168 9 45 343 68 391 1.27e-63 207
MS.gene83238.t1 MTR_2g087780 37.273 330 171 8 35 333 61 385 1.10e-62 205
MS.gene83238.t1 MTR_0003s0530 53.039 181 83 1 156 334 2 182 8.23e-59 189
MS.gene83238.t1 MTR_2g099030 34.718 337 188 6 35 341 70 404 9.38e-56 187
MS.gene83238.t1 MTR_2g058430 82.353 85 14 1 100 184 50 133 1.59e-39 140
MS.gene83238.t1 MTR_8g074210 31.915 235 135 4 47 281 78 287 1.17e-31 122
MS.gene83238.t1 MTR_4g112840 53.125 96 45 0 182 277 56 151 4.16e-30 113
MS.gene83238.t1 MTR_8g074270 31.646 237 137 4 45 281 76 287 5.58e-30 118
MS.gene83238.t1 MTR_8g074170 31.646 237 137 4 45 281 76 287 1.34e-28 114
MS.gene83238.t1 MTR_2g042430 31.048 248 142 7 120 343 126 368 7.97e-22 95.5
MS.gene83238.t1 MTR_2g042370 32.704 159 101 5 120 276 114 268 8.13e-18 84.0
MS.gene83238.t1 MTR_8g074320 36.154 130 79 3 105 232 92 219 5.97e-17 79.0
MS.gene83238.t1 MTR_1g075865 37.273 110 68 1 157 265 2 111 7.78e-17 76.6
MS.gene83238.t1 MTR_2g042420 31.447 159 102 5 120 276 107 260 1.81e-16 79.7
MS.gene83238.t1 MTR_5g077620 47.059 85 42 1 14 98 4 85 1.10e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83238.t1 AT1G74000 41.748 309 171 5 36 343 29 329 5.61e-78 242
MS.gene83238.t1 AT1G74020 42.444 311 166 6 36 343 27 327 1.82e-77 241
MS.gene83238.t1 AT1G74010 41.801 311 167 7 36 343 26 325 1.40e-72 228
MS.gene83238.t1 AT3G57030 38.315 368 186 9 1 333 3 364 2.21e-69 221
MS.gene83238.t1 AT5G22020 39.755 327 165 7 45 343 73 395 3.60e-66 214
MS.gene83238.t1 AT5G22020 39.755 327 165 7 45 343 73 395 3.60e-66 214
MS.gene83238.t1 AT5G22020 39.755 327 165 7 45 343 73 395 3.60e-66 214
MS.gene83238.t1 AT2G41290 37.463 339 175 9 38 343 42 376 9.16e-61 199
MS.gene83238.t1 AT3G57010 41.975 243 134 2 35 277 42 277 3.00e-60 198
MS.gene83238.t1 AT3G57020 39.241 237 137 3 41 277 47 276 5.58e-57 189
MS.gene83238.t1 AT1G08470 35.671 328 177 9 45 343 67 389 6.70e-57 189
MS.gene83238.t1 AT2G41300 36.304 303 160 5 57 333 88 383 5.81e-53 179
MS.gene83238.t1 AT3G59530 34.076 314 186 8 47 341 84 395 4.07e-51 175
MS.gene83238.t1 AT3G59530 34.076 314 186 8 47 341 84 395 4.07e-51 175
MS.gene83238.t1 AT3G59530 34.076 314 186 8 47 341 84 395 4.07e-51 175
MS.gene83238.t1 AT3G57020 36.287 237 130 2 41 277 47 262 2.40e-50 171
MS.gene83238.t1 AT3G51430 30.802 237 132 9 45 276 67 276 1.20e-23 100
MS.gene83238.t1 AT3G51430 30.769 234 136 9 45 276 67 276 1.23e-23 100
MS.gene83238.t1 AT3G51440 29.487 234 139 9 45 276 67 276 1.47e-22 97.4
MS.gene83238.t1 AT3G51420 29.536 237 135 9 45 276 67 276 4.00e-22 96.3
MS.gene83238.t1 AT3G51450 27.155 232 141 7 48 276 70 276 2.59e-21 94.0

Find 84 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATAGCTGATGCTTATTA+TGG 0.133630 2.4:+34329962 MS.gene83238:CDS
GGAACTCGATGAACGAGTTT+TGG 0.245358 2.4:+34330497 MS.gene83238:CDS
GAGTTAAATGCTAAGGATTC+AGG 0.258809 2.4:-34329063 None:intergenic
AATCTAGAAGGAAACCCTTT+TGG 0.273984 2.4:+34330037 MS.gene83238:CDS
GCAATCTAACTGAGGCATTT+GGG 0.280544 2.4:+34330332 MS.gene83238:CDS
TGTCCTTGTTAGCGAGTATA+AGG 0.290711 2.4:+34330381 MS.gene83238:CDS
TAGGCCTCCCACATGTTTCT+TGG 0.297657 2.4:-34329904 None:intergenic
CTAACAGAATCCGGAGATTC+TGG 0.310441 2.4:+34330404 MS.gene83238:CDS
GATATATTCTTGCGCCTTCC+TGG 0.316455 2.4:+34330454 MS.gene83238:CDS
CTGATGTTATCTGGTCTTCC+AGG 0.317283 2.4:-34330472 None:intergenic
TACAATGTGTAATGGCATTT+CGG 0.337550 2.4:+34329868 MS.gene83238:CDS
ATATAGCTGATGCTTATTAT+GGG 0.363334 2.4:+34329963 MS.gene83238:CDS
AGCAATCTAACTGAGGCATT+TGG 0.365757 2.4:+34330331 MS.gene83238:CDS
TATCTTCCATCACCACTAAC+TGG 0.365985 2.4:+34329039 MS.gene83238:CDS
GAGTTCCTTCTGATGTTATC+TGG 0.369443 2.4:-34330481 None:intergenic
AATGTAGTTTCGTTCGTTGA+TGG 0.373734 2.4:-34330301 None:intergenic
TAATCTGCAATAAGCAACTA+TGG 0.374570 2.4:-34329004 None:intergenic
TCCTTAGCATTTAACTCCAT+TGG 0.401838 2.4:+34329069 MS.gene83238:CDS
AAATAAACGATTCCAGTGTT+AGG 0.410992 2.4:-34330085 None:intergenic
CCATCAGAAACACCAGTATA+AGG 0.422560 2.4:-34329099 None:intergenic
AGCAACATAGCTGTTATCAT+TGG 0.424547 2.4:+34328949 MS.gene83238:CDS
CTTAGTCCTAGAGGCAATAC+AGG 0.430139 2.4:-34330565 None:intergenic
ATCCGGAGATTCTGGTTAAC+GGG 0.439072 2.4:+34330412 MS.gene83238:CDS
TGGATGATGAATAGAGTATG+AGG 0.447146 2.4:-34328984 None:intergenic
AGAAACATGTGGGAGGCCTA+TGG 0.451046 2.4:+34329907 MS.gene83238:CDS
ATATCTGAAGTATAAGCATT+AGG 0.451505 2.4:-34330436 None:intergenic
CTTGTGAAGGTTCCTTATGA+TGG 0.453532 2.4:+34329986 MS.gene83238:CDS
CTAGCTCACGTTACAAGATC+AGG 0.456249 2.4:+34330116 MS.gene83238:CDS
ATTGGTGAAGGACCTTATAC+TGG 0.459368 2.4:+34329087 MS.gene83238:CDS
AAGGATTCAGGGCCAGTTAG+TGG 0.476864 2.4:-34329051 None:intergenic
CCTTATACTGGTGTTTCTGA+TGG 0.479041 2.4:+34329099 MS.gene83238:CDS
AGTATAAGGCTAACAGAATC+CGG 0.480710 2.4:+34330395 MS.gene83238:CDS
GAAACATGTGGGAGGCCTAT+GGG 0.483364 2.4:+34329908 MS.gene83238:CDS
CACATAGACTCATACCTGTC+TGG 0.488706 2.4:-34329180 None:intergenic
ACCAATGGAGTTAAATGCTA+AGG 0.491313 2.4:-34329070 None:intergenic
GTCCCGTTAACCAGAATCTC+CGG 0.492891 2.4:-34330414 None:intergenic
GCAACATAGCTGTTATCATT+GGG 0.494734 2.4:+34328950 MS.gene83238:CDS
AATCCGGAGATTCTGGTTAA+CGG 0.495677 2.4:+34330411 MS.gene83238:CDS
TCAGGGCCAGTTAGTGGTGA+TGG 0.500584 2.4:-34329045 None:intergenic
AGAGGCAATACAGGAGGTAC+TGG 0.506539 2.4:-34330556 None:intergenic
TTGAGTTTCGACTACAACAC+AGG 0.517330 2.4:+34329932 MS.gene83238:CDS
CTTGTTGCTAATAATCTAGA+AGG 0.521330 2.4:+34330025 MS.gene83238:CDS
ACACCAGCAAGAAACCCAAA+AGG 0.523810 2.4:-34330052 None:intergenic
AGTTAAATGCTAAGGATTCA+GGG 0.528826 2.4:-34329062 None:intergenic
TTGCACACATTTCACCAGAC+AGG 0.531724 2.4:+34329166 MS.gene83238:CDS
GCATTTAACTCCATTGGTGA+AGG 0.536063 2.4:+34329075 MS.gene83238:CDS
GTTGCAGCACCACCATCATA+AGG 0.537567 2.4:-34329998 None:intergenic
ACTTAGGAGAAAAGATCATA+CGG 0.542640 2.4:+34330258 MS.gene83238:CDS
TGCTTATTATGGGCTTGTGA+AGG 0.543243 2.4:+34329973 MS.gene83238:CDS
GGGGTAGCAGTTAGCAACAA+TGG 0.560267 2.4:+34330352 MS.gene83238:CDS
GTACTAAACGCCTCAACAAG+AGG 0.560831 2.4:-34330622 None:intergenic
GTACCTCCTGTATTGCCTCT+AGG 0.564887 2.4:+34330559 MS.gene83238:CDS
AGGGACAATACAATGTGTAA+TGG 0.571321 2.4:+34329860 MS.gene83238:intron
GGTGGTGGTGAGGACGCAAA+AGG 0.573294 2.4:-34330535 None:intergenic
GGATGATGAATAGAGTATGA+GGG 0.576051 2.4:-34328983 None:intergenic
CAATCTAACTGAGGCATTTG+GGG 0.576466 2.4:+34330333 MS.gene83238:CDS
TGTTATCTGGTCTTCCAGGA+AGG 0.581662 2.4:-34330468 None:intergenic
TCAGAACTCCAAGAAACATG+TGG 0.584132 2.4:+34329896 MS.gene83238:CDS
CACCAGCAAGAAACCCAAAA+GGG 0.589802 2.4:-34330051 None:intergenic
TAGTCGAAACTCAACCCCAT+AGG 0.590652 2.4:-34329923 None:intergenic
GTGAAGGTTCCTTATGATGG+TGG 0.591324 2.4:+34329989 MS.gene83238:CDS
AGTATAAGGTCCTTCACCAA+TGG 0.592697 2.4:-34329085 None:intergenic
AATACAGGAGGTACTGGTGG+TGG 0.595158 2.4:-34330550 None:intergenic
AAACATGTGGGAGGCCTATG+GGG 0.604258 2.4:+34329909 MS.gene83238:CDS
AGAGTCTGTGCCTCTTGTTG+AGG 0.612871 2.4:+34330612 MS.gene83238:CDS
CAGTGAAGTTCAAGAATCCG+AGG 0.615872 2.4:+34330654 MS.gene83238:CDS
GAAGACCAGATAACATCAGA+AGG 0.618915 2.4:+34330476 MS.gene83238:CDS
GTGAAATGTGTGCAAACTCA+AGG 0.621379 2.4:-34329158 None:intergenic
CTTAGGAGAAAAGATCATAC+GGG 0.622464 2.4:+34330259 MS.gene83238:CDS
AGGAGGTACTGGTGGTGGTG+AGG 0.623213 2.4:-34330545 None:intergenic
GGACTAAGAGTCAATGCGGA+AGG 0.626124 2.4:+34330580 MS.gene83238:CDS
TAGCCTTATACTCGCTAACA+AGG 0.631951 2.4:-34330384 None:intergenic
TGAGTTTCGACTACAACACA+GGG 0.637847 2.4:+34329933 MS.gene83238:CDS
GCATTGACTCTTAGTCCTAG+AGG 0.638246 2.4:-34330574 None:intergenic
AGTGAAGTTCAAGAATCCGA+GGG 0.641761 2.4:+34330655 MS.gene83238:CDS
GGCAATACAGGAGGTACTGG+TGG 0.650197 2.4:-34330553 None:intergenic
TGTAGCATAGAGTCTTCCCT+CGG 0.651979 2.4:-34330671 None:intergenic
CAGAACTCCAAGAAACATGT+GGG 0.662175 2.4:+34329897 MS.gene83238:CDS
GTGGATGTAGATCCTAACAC+TGG 0.675066 2.4:+34330073 MS.gene83238:CDS
AACTCCAAGAAACATGTGGG+AGG 0.685654 2.4:+34329900 MS.gene83238:CDS
AGTCCTAGAGGCAATACAGG+AGG 0.688689 2.4:-34330562 None:intergenic
GTTGCTCAGCAATCTAACTG+AGG 0.711728 2.4:+34330324 MS.gene83238:CDS
TCTAGGACTAAGAGTCAATG+CGG 0.760927 2.4:+34330576 MS.gene83238:CDS
GAGTTTCGACTACAACACAG+GGG 0.803781 2.4:+34329934 MS.gene83238:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACCTTTAAAACTAAAAAA+TGG - chr2.4:34329293-34329312 None:intergenic 15.0%
!!! TATCCATTTTTTAGTTTTAA+AGG + chr2.4:34329287-34329306 MS.gene83238:intron 15.0%
!! AAGCAAAACTTATATTAAGA+AGG + chr2.4:34329338-34329357 MS.gene83238:intron 20.0%
!! AGCAAAACTTATATTAAGAA+GGG + chr2.4:34329339-34329358 MS.gene83238:intron 20.0%
!! CTGTTGTTATATAAATAGAA+TGG + chr2.4:34329213-34329232 MS.gene83238:intron 20.0%
!!! AGTTTTGCTTCTTTAAAAAA+AGG - chr2.4:34329328-34329347 None:intergenic 20.0%
!!! TAAGGGTATTTTTTTCAAAT+AGG - chr2.4:34329638-34329657 None:intergenic 20.0%
!!! TTCTTTAAAAAAAGGTTTAG+AGG - chr2.4:34329320-34329339 None:intergenic 20.0%
!!! TTTTCAAATAGGTTTTGAAA+TGG - chr2.4:34329627-34329646 None:intergenic 20.0%
! AAAAATACCCTTATAGTAGA+AGG + chr2.4:34329645-34329664 MS.gene83238:intron 25.0%
! AAAATACCCTTATAGTAGAA+GGG + chr2.4:34329646-34329665 MS.gene83238:intron 25.0%
! AACTTATATTAAGAAGGGAA+AGG + chr2.4:34329344-34329363 MS.gene83238:intron 25.0%
! ATATAGCTGATGCTTATTAT+GGG + chr2.4:34329963-34329982 MS.gene83238:CDS 25.0%
! GTGTTTGTCAATTATAATTG+TGG + chr2.4:34330204-34330223 MS.gene83238:intron 25.0%
! TATATAGCTGATGCTTATTA+TGG + chr2.4:34329962-34329981 MS.gene83238:CDS 25.0%
!! ATATCTGAAGTATAAGCATT+AGG - chr2.4:34330439-34330458 None:intergenic 25.0%
!! TCTTTTCATGTAACTAAAGT+TGG + chr2.4:34329808-34329827 MS.gene83238:intron 25.0%
AAATAAACGATTCCAGTGTT+AGG - chr2.4:34330088-34330107 None:intergenic 30.0%
ACTTAGGAGAAAAGATCATA+CGG + chr2.4:34330258-34330277 MS.gene83238:CDS 30.0%
AGGTTAAATCAAGACATTGT+TGG + chr2.4:34329834-34329853 MS.gene83238:intron 30.0%
AGTTAAATGCTAAGGATTCA+GGG - chr2.4:34329065-34329084 None:intergenic 30.0%
ATCGCAGTTCAAAATATTGT+CGG - chr2.4:34329246-34329265 None:intergenic 30.0%
ATTCGAACTCAACATATTGT+GGG - chr2.4:34329683-34329702 None:intergenic 30.0%
CTTGTTGCTAATAATCTAGA+AGG + chr2.4:34330025-34330044 MS.gene83238:CDS 30.0%
TAATCTGCAATAAGCAACTA+TGG - chr2.4:34329007-34329026 None:intergenic 30.0%
TACAATGTGTAATGGCATTT+CGG + chr2.4:34329868-34329887 MS.gene83238:CDS 30.0%
TATTCGAACTCAACATATTG+TGG - chr2.4:34329684-34329703 None:intergenic 30.0%
TGAGTAGATAGTAGATTCTA+TGG - chr2.4:34329566-34329585 None:intergenic 30.0%
! CTTTTCTCCTAAGTAGTTTT+TGG - chr2.4:34330252-34330271 None:intergenic 30.0%
!! GTATTTCTAGACATCATTAG+CGG - chr2.4:34329540-34329559 None:intergenic 30.0%
!! TGTCGTATGTTCTTAGTTTT+AGG - chr2.4:34329404-34329423 None:intergenic 30.0%
!!! ATAGGTTTTGAAATGGCTTT+TGG - chr2.4:34329620-34329639 None:intergenic 30.0%
AATCAAGACATTGTTGGTAC+AGG + chr2.4:34329840-34329859 MS.gene83238:intron 35.0%
AATGTAGTTTCGTTCGTTGA+TGG - chr2.4:34330304-34330323 None:intergenic 35.0%
ACCAATGGAGTTAAATGCTA+AGG - chr2.4:34329073-34329092 None:intergenic 35.0%
AGATCACCCTTCTACTATAA+GGG - chr2.4:34329655-34329674 None:intergenic 35.0%
AGCAACATAGCTGTTATCAT+TGG + chr2.4:34328949-34328968 MS.gene83238:CDS 35.0%
AGGGACAATACAATGTGTAA+TGG + chr2.4:34329860-34329879 MS.gene83238:intron 35.0%
AGGTATGAGTCTATGTGTAA+TGG + chr2.4:34329186-34329205 MS.gene83238:intron 35.0%
AGTATAAGGCTAACAGAATC+CGG + chr2.4:34330395-34330414 MS.gene83238:CDS 35.0%
ATCAAGACATTGTTGGTACA+GGG + chr2.4:34329841-34329860 MS.gene83238:intron 35.0%
CTTAGGAGAAAAGATCATAC+GGG + chr2.4:34330259-34330278 MS.gene83238:CDS 35.0%
GAGTTAAATGCTAAGGATTC+AGG - chr2.4:34329066-34329085 None:intergenic 35.0%
GCAACATAGCTGTTATCATT+GGG + chr2.4:34328950-34328969 MS.gene83238:CDS 35.0%
GCTACAACAGAAATCACAAA+AGG + chr2.4:34329468-34329487 MS.gene83238:intron 35.0%
GGATGATGAATAGAGTATGA+GGG - chr2.4:34328986-34329005 None:intergenic 35.0%
TAACATGAACGTAGATCCTA+TGG + chr2.4:34329746-34329765 MS.gene83238:intron 35.0%
TCCTTAGCATTTAACTCCAT+TGG + chr2.4:34329069-34329088 MS.gene83238:CDS 35.0%
TGGATGATGAATAGAGTATG+AGG - chr2.4:34328987-34329006 None:intergenic 35.0%
TGTGGAACCAAATATTTGCA+GGG + chr2.4:34330222-34330241 MS.gene83238:intron 35.0%
TTCGAACTCAACATATTGTG+GGG - chr2.4:34329682-34329701 None:intergenic 35.0%
TTGTGGAACCAAATATTTGC+AGG + chr2.4:34330221-34330240 MS.gene83238:intron 35.0%
! AATCTAGAAGGAAACCCTTT+TGG + chr2.4:34330037-34330056 MS.gene83238:CDS 35.0%
! ATCTAGAAGGAAACCCTTTT+GGG + chr2.4:34330038-34330057 MS.gene83238:CDS 35.0%
! CATGTAACTAAAGTTGGTTG+AGG + chr2.4:34329814-34329833 MS.gene83238:intron 35.0%
AGTATAAGGTCCTTCACCAA+TGG - chr2.4:34329088-34329107 None:intergenic 40.0%
AGTGAAGTTCAAGAATCCGA+GGG + chr2.4:34330655-34330674 MS.gene83238:CDS 40.0%
ATTGGTGAAGGACCTTATAC+TGG + chr2.4:34329087-34329106 MS.gene83238:CDS 40.0%
CAGAACTCCAAGAAACATGT+GGG + chr2.4:34329897-34329916 MS.gene83238:CDS 40.0%
CAGATCACCCTTCTACTATA+AGG - chr2.4:34329656-34329675 None:intergenic 40.0%
CCATCAGAAACACCAGTATA+AGG - chr2.4:34329102-34329121 None:intergenic 40.0%
CTTATAGTAGAAGGGTGATC+TGG + chr2.4:34329654-34329673 MS.gene83238:intron 40.0%
CTTGTGAAGGTTCCTTATGA+TGG + chr2.4:34329986-34330005 MS.gene83238:CDS 40.0%
GAAGACCAGATAACATCAGA+AGG + chr2.4:34330476-34330495 MS.gene83238:CDS 40.0%
GAGTTCCTTCTGATGTTATC+TGG - chr2.4:34330484-34330503 None:intergenic 40.0%
GCATTTAACTCCATTGGTGA+AGG + chr2.4:34329075-34329094 MS.gene83238:CDS 40.0%
GGGATCTCCAAAAACTACTT+AGG + chr2.4:34330242-34330261 MS.gene83238:intron 40.0%
GTGAAATGTGTGCAAACTCA+AGG - chr2.4:34329161-34329180 None:intergenic 40.0%
TATCTTCCATCACCACTAAC+TGG + chr2.4:34329039-34329058 MS.gene83238:CDS 40.0%
TCAGAACTCCAAGAAACATG+TGG + chr2.4:34329896-34329915 MS.gene83238:CDS 40.0%
TCGAACTCAACATATTGTGG+GGG - chr2.4:34329681-34329700 None:intergenic 40.0%
TGAGTTTCGACTACAACACA+GGG + chr2.4:34329933-34329952 MS.gene83238:CDS 40.0%
TGCTTATTATGGGCTTGTGA+AGG + chr2.4:34329973-34329992 MS.gene83238:CDS 40.0%
TGGAGATCCCTGCAAATATT+TGG - chr2.4:34330232-34330251 None:intergenic 40.0%
TGTCCTTGTTAGCGAGTATA+AGG + chr2.4:34330381-34330400 MS.gene83238:CDS 40.0%
TTGAGTTTCGACTACAACAC+AGG + chr2.4:34329932-34329951 MS.gene83238:CDS 40.0%
! AATCCGGAGATTCTGGTTAA+CGG + chr2.4:34330411-34330430 MS.gene83238:CDS 40.0%
! AGCAATCTAACTGAGGCATT+TGG + chr2.4:34330331-34330350 MS.gene83238:CDS 40.0%
! CAATCTAACTGAGGCATTTG+GGG + chr2.4:34330333-34330352 MS.gene83238:CDS 40.0%
! CCTTATACTGGTGTTTCTGA+TGG + chr2.4:34329099-34329118 MS.gene83238:CDS 40.0%
! GAACTCGATGAACGAGTTTT+GGG + chr2.4:34330498-34330517 MS.gene83238:CDS 40.0%
! GCAATCTAACTGAGGCATTT+GGG + chr2.4:34330332-34330351 MS.gene83238:CDS 40.0%
! TCTAGGACTAAGAGTCAATG+CGG + chr2.4:34330576-34330595 MS.gene83238:CDS 40.0%
!! TAGCCTTATACTCGCTAACA+AGG - chr2.4:34330387-34330406 None:intergenic 40.0%
AACTCCAAGAAACATGTGGG+AGG + chr2.4:34329900-34329919 MS.gene83238:CDS 45.0%
ACACCAGCAAGAAACCCAAA+AGG - chr2.4:34330055-34330074 None:intergenic 45.0%
CACATAGACTCATACCTGTC+TGG - chr2.4:34329183-34329202 None:intergenic 45.0%
CACCAGCAAGAAACCCAAAA+GGG - chr2.4:34330054-34330073 None:intergenic 45.0%
CAGTGAAGTTCAAGAATCCG+AGG + chr2.4:34330654-34330673 MS.gene83238:CDS 45.0%
CTAACAGAATCCGGAGATTC+TGG + chr2.4:34330404-34330423 MS.gene83238:CDS 45.0%
CTAGCTCACGTTACAAGATC+AGG + chr2.4:34330116-34330135 MS.gene83238:CDS 45.0%
CTTAGTCCTAGAGGCAATAC+AGG - chr2.4:34330568-34330587 None:intergenic 45.0%
GAACATGCGAACGAGTAGAA+TGG - chr2.4:34330146-34330165 None:intergenic 45.0%
GAGTTTCGACTACAACACAG+GGG + chr2.4:34329934-34329953 MS.gene83238:CDS 45.0%
GATATATTCTTGCGCCTTCC+TGG + chr2.4:34330454-34330473 MS.gene83238:CDS 45.0%
GTACTAAACGCCTCAACAAG+AGG - chr2.4:34330625-34330644 None:intergenic 45.0%
GTGAAGGTTCCTTATGATGG+TGG + chr2.4:34329989-34330008 MS.gene83238:CDS 45.0%
GTGGATGTAGATCCTAACAC+TGG + chr2.4:34330073-34330092 MS.gene83238:CDS 45.0%
GTTGCTCAGCAATCTAACTG+AGG + chr2.4:34330324-34330343 MS.gene83238:CDS 45.0%
TAGTCGAAACTCAACCCCAT+AGG - chr2.4:34329926-34329945 None:intergenic 45.0%
TGTAGCATAGAGTCTTCCCT+CGG - chr2.4:34330674-34330693 None:intergenic 45.0%
TTGCACACATTTCACCAGAC+AGG + chr2.4:34329166-34329185 MS.gene83238:CDS 45.0%
! ATCCGGAGATTCTGGTTAAC+GGG + chr2.4:34330412-34330431 MS.gene83238:CDS 45.0%
! CTGATGTTATCTGGTCTTCC+AGG - chr2.4:34330475-34330494 None:intergenic 45.0%
! GGAACTCGATGAACGAGTTT+TGG + chr2.4:34330497-34330516 MS.gene83238:CDS 45.0%
! TGTTATCTGGTCTTCCAGGA+AGG - chr2.4:34330471-34330490 None:intergenic 45.0%
!! GCATTGACTCTTAGTCCTAG+AGG - chr2.4:34330577-34330596 None:intergenic 45.0%
!!! AACCCTTTTGGGTTTCTTGC+TGG + chr2.4:34330049-34330068 MS.gene83238:CDS 45.0%
!!! TTTTGGGTTTCTTGCTGGTG+TGG + chr2.4:34330054-34330073 MS.gene83238:CDS 45.0%
!!! GAAATATATATTATATTTTA+TGG - chr2.4:34329790-34329809 None:intergenic 5.0%
AAACATGTGGGAGGCCTATG+GGG + chr2.4:34329909-34329928 MS.gene83238:CDS 50.0%
AAGGATTCAGGGCCAGTTAG+TGG - chr2.4:34329054-34329073 None:intergenic 50.0%
AGAAACATGTGGGAGGCCTA+TGG + chr2.4:34329907-34329926 MS.gene83238:CDS 50.0%
AGAGTCTGTGCCTCTTGTTG+AGG + chr2.4:34330612-34330631 MS.gene83238:CDS 50.0%
AGTCCTAGAGGCAATACAGG+AGG - chr2.4:34330565-34330584 None:intergenic 50.0%
GAAACATGTGGGAGGCCTAT+GGG + chr2.4:34329908-34329927 MS.gene83238:CDS 50.0%
GGACTAAGAGTCAATGCGGA+AGG + chr2.4:34330580-34330599 MS.gene83238:CDS 50.0%
GGGGTAGCAGTTAGCAACAA+TGG + chr2.4:34330352-34330371 MS.gene83238:CDS 50.0%
GTACCTCCTGTATTGCCTCT+AGG + chr2.4:34330559-34330578 MS.gene83238:CDS 50.0%
GTCCCGTTAACCAGAATCTC+CGG - chr2.4:34330417-34330436 None:intergenic 50.0%
GTTGCAGCACCACCATCATA+AGG - chr2.4:34330001-34330020 None:intergenic 50.0%
TAGGCCTCCCACATGTTTCT+TGG - chr2.4:34329907-34329926 None:intergenic 50.0%
! AGAGGCAATACAGGAGGTAC+TGG - chr2.4:34330559-34330578 None:intergenic 50.0%
!! AATACAGGAGGTACTGGTGG+TGG - chr2.4:34330553-34330572 None:intergenic 50.0%
!!! CTCGATGAACGAGTTTTGGG+TGG + chr2.4:34330501-34330520 MS.gene83238:CDS 50.0%
AAACCACGCGTCTGCTCCAT+AGG - chr2.4:34329765-34329784 None:intergenic 55.0%
TCAGGGCCAGTTAGTGGTGA+TGG - chr2.4:34329048-34329067 None:intergenic 55.0%
!! GGCAATACAGGAGGTACTGG+TGG - chr2.4:34330556-34330575 None:intergenic 55.0%
GATCCTATGGAGCAGACGCG+TGG + chr2.4:34329759-34329778 MS.gene83238:intron 60.0%
! GGTGGTGGTGAGGACGCAAA+AGG - chr2.4:34330538-34330557 None:intergenic 60.0%
!! AGGAGGTACTGGTGGTGGTG+AGG - chr2.4:34330548-34330567 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 34328922 34330732 34328922 ID=MS.gene83238
chr2.4 mRNA 34328922 34330732 34328922 ID=MS.gene83238.t1;Parent=MS.gene83238
chr2.4 exon 34328922 34329187 34328922 ID=MS.gene83238.t1.exon1;Parent=MS.gene83238.t1
chr2.4 CDS 34328922 34329187 34328922 ID=cds.MS.gene83238.t1;Parent=MS.gene83238.t1
chr2.4 exon 34329862 34330137 34329862 ID=MS.gene83238.t1.exon2;Parent=MS.gene83238.t1
chr2.4 CDS 34329862 34330137 34329862 ID=cds.MS.gene83238.t1;Parent=MS.gene83238.t1
chr2.4 exon 34330243 34330732 34330243 ID=MS.gene83238.t1.exon3;Parent=MS.gene83238.t1
chr2.4 CDS 34330243 34330732 34330243 ID=cds.MS.gene83238.t1;Parent=MS.gene83238.t1
Gene Sequence

>MS.gene83238

ATGTTGGTCATCATGTTGAACATGTCTAGCAACATAGCTGTTATCATTGGGATCTTAATCTCCCTCATACTCTATTCATCATCCATAGTTGCTTATTGCAGATTACTTGATAGACTTTATCTTCCATCACCACTAACTGGCCCTGAATCCTTAGCATTTAACTCCATTGGTGAAGGACCTTATACTGGTGTTTCTGATGGAAGAATTCTTAAATATGACGAAGAATGTTCTTGTTTCCTTGAGTTTGCACACATTTCACCAGACAGGTATGAGTCTATGTGTAATGGCTGTCTGTTGTTATATAAATAGAATGGTTTAACTCCGACAATATTTTGAACTGCGATTCGAGTATCTGTCATGAGAGATATCCATTTTTTAGTTTTAAAGGTATGTATCCTCTAAACCTTTTTTTAAAGAAGCAAAACTTATATTAAGAAGGGAAAGGTATGTGTTTAGTGAAAACTTTTTTATTTTCATTTCCTAAAACTAAGAACATACGACATGTATGTCTCGTGAAAATATTTTCTATTTTCAACTCGCATACACGCTACAACAGAAATCACAAAAGGAAAGATATATGACAACAAAATCAGAAATAAAATATCGACAAACTCGCCGCTAATGATGTCTAGAAATACATTCCATAGAATCTACTATCTACTCATAAAAGAGACAAGCTACAAATTTAGATCGAGCCAAAAGCCATTTCAAAACCTATTTGAAAAAAATACCCTTATAGTAGAAGGGTGATCTGGTCCCCCACAATATGTTGAGTTCGAATATCTGCTACGAGAGATGTCTATGTTTAATATCAACAACTCTTCTAACATGAACGTAGATCCTATGGAGCAGACGCGTGGTTTCACCATAAAATATAATATATATTTCTTTTCATGTAACTAAAGTTGGTTGAGGTTAAATCAAGACATTGTTGGTACAGGGACAATACAATGTGTAATGGCATTTCGGACTTCTCAGAACTCCAAGAAACATGTGGGAGGCCTATGGGGTTGAGTTTCGACTACAACACAGGGGAATTATATATAGCTGATGCTTATTATGGGCTTGTGAAGGTTCCTTATGATGGTGGTGCTGCAACACAACTTGTTGCTAATAATCTAGAAGGAAACCCTTTTGGGTTTCTTGCTGGTGTGGATGTAGATCCTAACACTGGAATCGTTTATTTCACAGAAGCTAGCTCACGTTACAAGATCAGGTAATCCATTCTACTCGTTCGCATGTTCATACAATAAATTGTTCATTATATCACATGTTCTATCAAGTGTTTGTCAATTATAATTGTGGAACCAAATATTTGCAGGGATCTCCAAAAACTACTTAGGAGAAAAGATCATACGGGAAGCTTATTCAGATACGATCCATCAACGAACGAAACTACATTGTTGCTCAGCAATCTAACTGAGGCATTTGGGGTAGCAGTTAGCAACAATGGTTCATTTGTCCTTGTTAGCGAGTATAAGGCTAACAGAATCCGGAGATTCTGGTTAACGGGACCTAATGCTTATACTTCAGATATATTCTTGCGCCTTCCTGGAAGACCAGATAACATCAGAAGGAACTCGATGAACGAGTTTTGGGTGGCAGTGAATTATCCTTTTGCGTCCTCACCACCACCAGTACCTCCTGTATTGCCTCTAGGACTAAGAGTCAATGCGGAAGGTTTAATTATAGAGTCTGTGCCTCTTGTTGAGGCGTTTAGTACTGAATCAGTCAGTGAAGTTCAAGAATCCGAGGGAAGACTCTATGCTACATCGTTGAGTGATAACTATGCTAGCATCCTCAAGCTCTAG
Protein sequence

>MS.gene83238.t1

MLVIMLNMSSNIAVIIGILISLILYSSSIVAYCRLLDRLYLPSPLTGPESLAFNSIGEGPYTGVSDGRILKYDEECSCFLEFAHISPDRDNTMCNGISDFSELQETCGRPMGLSFDYNTGELYIADAYYGLVKVPYDGGAATQLVANNLEGNPFGFLAGVDVDPNTGIVYFTEASSRYKIRDLQKLLRRKDHTGSLFRYDPSTNETTLLLSNLTEAFGVAVSNNGSFVLVSEYKANRIRRFWLTGPNAYTSDIFLRLPGRPDNIRRNSMNEFWVAVNYPFASSPPPVPPVLPLGLRVNAEGLIIESVPLVEAFSTESVSEVQESEGRLYATSLSDNYASILKL