Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68975.t1 | XP_003609161.1 | 91.7 | 326 | 25 | 2 | 7 | 331 | 2 | 326 | 2.90E-162 | 581.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68975.t1 | P94111 | 41.8 | 328 | 175 | 8 | 7 | 326 | 3 | 322 | 3.4e-58 | 226.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68975.t1 | G7JT36 | 91.7 | 326 | 25 | 2 | 7 | 331 | 2 | 326 | 2.1e-162 | 581.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene68975 | 0.806191 | 9.27E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene68975 | 0.802403 | 5.70E-49 | -1.69E-46 |
| MS.gene050585 | MS.gene68975 | 0.803746 | 3.01E-49 | -1.69E-46 |
| MS.gene050780 | MS.gene68975 | 0.830012 | 3.73E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene68975 | 0.810017 | 1.42E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene68975 | 0.837827 | 4.13E-57 | -1.69E-46 |
| MS.gene052142 | MS.gene68975 | 0.823188 | 1.58E-53 | -1.69E-46 |
| MS.gene052698 | MS.gene68975 | 0.826699 | 2.35E-54 | -1.69E-46 |
| MS.gene053074 | MS.gene68975 | 0.811638 | 6.33E-51 | -1.69E-46 |
| MS.gene053563 | MS.gene68975 | 0.818291 | 2.12E-52 | -1.69E-46 |
| MS.gene054596 | MS.gene68975 | 0.81206 | 5.13E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene68975 | 0.80312 | 4.06E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene68975 | 0.803466 | 3.44E-49 | -1.69E-46 |
| MS.gene059006 | MS.gene68975 | 0.823444 | 1.38E-53 | -1.69E-46 |
| MS.gene059632 | MS.gene68975 | 0.810896 | 9.18E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene68975 | 0.80465 | 1.95E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08295 | MS.gene68975 | PPI |
| MS.gene045791 | MS.gene68975 | PPI |
| MS.gene023328 | MS.gene68975 | PPI |
| MS.gene83238 | MS.gene68975 | PPI |
| MS.gene68976 | MS.gene68975 | PPI |
| MS.gene78185 | MS.gene68975 | PPI |
| MS.gene033026 | MS.gene68975 | PPI |
| MS.gene045793 | MS.gene68975 | PPI |
| MS.gene033012 | MS.gene68975 | PPI |
| MS.gene038810 | MS.gene68975 | PPI |
| MS.gene038798 | MS.gene68975 | PPI |
| MS.gene78183 | MS.gene68975 | PPI |
| MS.gene044490 | MS.gene68975 | PPI |
| MS.gene68977 | MS.gene68975 | PPI |
| MS.gene050115 | MS.gene68975 | PPI |
| MS.gene033013 | MS.gene68975 | PPI |
| MS.gene68975 | MS.gene063529 | PPI |
| MS.gene34908 | MS.gene68975 | PPI |
| MS.gene063529 | MS.gene68975 | PPI |
| MS.gene83236 | MS.gene68975 | PPI |
| MS.gene023327 | MS.gene68975 | PPI |
| MS.gene08297 | MS.gene68975 | PPI |
| MS.gene044500 | MS.gene68975 | PPI |
| MS.gene044502 | MS.gene68975 | PPI |
| MS.gene35843 | MS.gene68975 | PPI |
| MS.gene025506 | MS.gene68975 | PPI |
| MS.gene044501 | MS.gene68975 | PPI |
| MS.gene033015 | MS.gene68975 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68975.t1 | MTR_4g112630 | 91.718 | 326 | 25 | 2 | 7 | 331 | 2 | 326 | 0.0 | 593 |
| MS.gene68975.t1 | MTR_4g112650 | 79.403 | 335 | 56 | 4 | 7 | 330 | 2 | 334 | 0.0 | 521 |
| MS.gene68975.t1 | MTR_4g112620 | 77.844 | 334 | 36 | 3 | 1 | 330 | 1 | 300 | 0.0 | 505 |
| MS.gene68975.t1 | MTR_4g112670 | 74.854 | 342 | 65 | 6 | 1 | 329 | 1 | 334 | 7.54e-179 | 498 |
| MS.gene68975.t1 | MTR_2g058450 | 54.341 | 311 | 127 | 4 | 30 | 329 | 28 | 334 | 1.86e-107 | 317 |
| MS.gene68975.t1 | MTR_0003s0530 | 70.811 | 185 | 38 | 4 | 149 | 321 | 1 | 181 | 3.83e-82 | 248 |
| MS.gene68975.t1 | MTR_7g490330 | 43.448 | 290 | 151 | 6 | 42 | 330 | 47 | 324 | 4.20e-73 | 229 |
| MS.gene68975.t1 | MTR_2g058410 | 52.766 | 235 | 98 | 3 | 106 | 330 | 1 | 232 | 2.67e-70 | 219 |
| MS.gene68975.t1 | MTR_7g490350 | 37.771 | 323 | 158 | 7 | 42 | 329 | 53 | 367 | 3.96e-69 | 220 |
| MS.gene68975.t1 | MTR_5g091980 | 38.344 | 326 | 185 | 6 | 7 | 330 | 11 | 322 | 7.60e-68 | 215 |
| MS.gene68975.t1 | MTR_7g090320 | 38.596 | 342 | 162 | 8 | 32 | 329 | 48 | 385 | 1.39e-67 | 217 |
| MS.gene68975.t1 | MTR_7g090300 | 35.958 | 381 | 174 | 10 | 6 | 330 | 2 | 368 | 2.97e-66 | 213 |
| MS.gene68975.t1 | MTR_2g087780 | 36.550 | 342 | 168 | 8 | 29 | 329 | 60 | 393 | 8.20e-64 | 207 |
| MS.gene68975.t1 | MTR_7g090310 | 37.160 | 331 | 161 | 7 | 42 | 329 | 58 | 384 | 5.96e-62 | 202 |
| MS.gene68975.t1 | MTR_4g050190 | 35.103 | 339 | 170 | 8 | 32 | 329 | 60 | 389 | 5.34e-61 | 200 |
| MS.gene68975.t1 | MTR_2g099030 | 33.036 | 336 | 180 | 6 | 30 | 325 | 70 | 400 | 1.33e-54 | 184 |
| MS.gene68975.t1 | MTR_4g112840 | 67.327 | 101 | 30 | 1 | 176 | 276 | 56 | 153 | 2.74e-39 | 137 |
| MS.gene68975.t1 | MTR_5g077620 | 72.619 | 84 | 21 | 2 | 8 | 91 | 1 | 82 | 3.02e-34 | 123 |
| MS.gene68975.t1 | MTR_8g074210 | 39.181 | 171 | 98 | 4 | 103 | 273 | 118 | 282 | 3.23e-27 | 110 |
| MS.gene68975.t1 | MTR_8g074170 | 38.596 | 171 | 99 | 4 | 103 | 273 | 118 | 282 | 1.73e-25 | 105 |
| MS.gene68975.t1 | MTR_8g074270 | 38.012 | 171 | 100 | 4 | 103 | 273 | 118 | 282 | 2.90e-25 | 104 |
| MS.gene68975.t1 | MTR_2g042430 | 31.381 | 239 | 134 | 8 | 40 | 277 | 74 | 283 | 3.63e-24 | 101 |
| MS.gene68975.t1 | MTR_2g058430 | 50.485 | 103 | 42 | 1 | 79 | 181 | 43 | 136 | 2.39e-23 | 97.4 |
| MS.gene68975.t1 | MTR_2g042370 | 33.133 | 166 | 105 | 4 | 113 | 277 | 112 | 272 | 1.60e-20 | 91.3 |
| MS.gene68975.t1 | MTR_2g042420 | 28.033 | 239 | 142 | 8 | 40 | 277 | 55 | 264 | 7.01e-20 | 89.4 |
| MS.gene68975.t1 | MTR_8g074320 | 38.462 | 130 | 72 | 4 | 103 | 229 | 95 | 219 | 2.33e-18 | 82.8 |
| MS.gene68975.t1 | MTR_1g075865 | 40.541 | 111 | 62 | 2 | 153 | 262 | 4 | 111 | 2.92e-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68975.t1 | AT1G74020 | 43.974 | 307 | 156 | 8 | 31 | 329 | 27 | 325 | 1.91e-71 | 225 |
| MS.gene68975.t1 | AT3G57030 | 40.187 | 321 | 147 | 6 | 42 | 325 | 56 | 368 | 1.08e-70 | 224 |
| MS.gene68975.t1 | AT1G74010 | 43.421 | 304 | 155 | 9 | 31 | 326 | 26 | 320 | 3.70e-69 | 219 |
| MS.gene68975.t1 | AT1G74000 | 41.311 | 305 | 167 | 7 | 31 | 329 | 29 | 327 | 1.24e-66 | 213 |
| MS.gene68975.t1 | AT3G57010 | 38.235 | 272 | 152 | 4 | 11 | 275 | 16 | 278 | 2.35e-63 | 206 |
| MS.gene68975.t1 | AT5G22020 | 37.195 | 328 | 161 | 9 | 40 | 329 | 73 | 393 | 7.26e-63 | 205 |
| MS.gene68975.t1 | AT5G22020 | 37.195 | 328 | 161 | 9 | 40 | 329 | 73 | 393 | 7.26e-63 | 205 |
| MS.gene68975.t1 | AT5G22020 | 37.195 | 328 | 161 | 9 | 40 | 329 | 73 | 393 | 7.26e-63 | 205 |
| MS.gene68975.t1 | AT1G08470 | 36.391 | 338 | 166 | 9 | 32 | 329 | 59 | 387 | 1.84e-62 | 204 |
| MS.gene68975.t1 | AT3G57020 | 40.000 | 240 | 135 | 2 | 36 | 275 | 47 | 277 | 1.66e-59 | 195 |
| MS.gene68975.t1 | AT2G41290 | 36.446 | 332 | 159 | 11 | 42 | 329 | 51 | 374 | 1.13e-58 | 193 |
| MS.gene68975.t1 | AT2G41300 | 39.301 | 229 | 130 | 3 | 47 | 275 | 83 | 302 | 7.52e-54 | 181 |
| MS.gene68975.t1 | AT3G57020 | 37.917 | 240 | 126 | 2 | 36 | 275 | 47 | 263 | 7.80e-54 | 180 |
| MS.gene68975.t1 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 6.13e-53 | 179 |
| MS.gene68975.t1 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 6.13e-53 | 179 |
| MS.gene68975.t1 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 6.13e-53 | 179 |
| MS.gene68975.t1 | AT3G51440 | 27.586 | 290 | 177 | 8 | 20 | 301 | 40 | 304 | 6.43e-23 | 98.2 |
| MS.gene68975.t1 | AT3G51430 | 35.028 | 177 | 106 | 5 | 103 | 278 | 113 | 281 | 2.34e-21 | 94.0 |
| MS.gene68975.t1 | AT3G51430 | 35.057 | 174 | 104 | 5 | 103 | 275 | 113 | 278 | 1.02e-20 | 92.0 |
| MS.gene68975.t1 | AT3G51450 | 30.732 | 205 | 125 | 6 | 103 | 302 | 113 | 305 | 5.64e-20 | 90.1 |
| MS.gene68975.t1 | AT3G51420 | 29.353 | 201 | 133 | 5 | 103 | 302 | 113 | 305 | 8.08e-20 | 89.4 |
Find 87 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATTCAAGAGGCCAATTT+TGG | 0.175988 | 8.1:-15253480 | MS.gene68975:CDS |
| TATGCAGCTGATGCTTATTA+TGG | 0.243078 | 8.1:-15254308 | MS.gene68975:CDS |
| GAATTCCTCTTTATGTTATC+TGG | 0.262678 | 8.1:+15253496 | None:intergenic |
| CTTGTGCAGGTCCAACTAAT+TGG | 0.268148 | 8.1:+15254249 | None:intergenic |
| TATTAACTGCAATCCAAAAT+TGG | 0.282710 | 8.1:+15253467 | None:intergenic |
| AAATCTGAGGAATTTGCTTT+TGG | 0.297207 | 8.1:+15253541 | None:intergenic |
| GTTGAACATTAATAATTCTA+TGG | 0.304772 | 8.1:-15254793 | MS.gene68975:CDS |
| CTGAAGCTTGTTCAGTAATA+TGG | 0.311548 | 8.1:+15254713 | None:intergenic |
| TTGGGTTGCTTTCCCTCCAT+TGG | 0.315305 | 8.1:+15254268 | None:intergenic |
| AAATAAACGATTCCAGTGTT+TGG | 0.321439 | 8.1:+15254188 | None:intergenic |
| TGGGTTGCTTTCCCTCCATT+GGG | 0.331282 | 8.1:+15254269 | None:intergenic |
| AGAAATCTTAAATTTGCATC+TGG | 0.338821 | 8.1:-15253646 | MS.gene68975:CDS |
| TAATAGTCTTACTTTGTCTT+CGG | 0.340893 | 8.1:-15253449 | MS.gene68975:CDS |
| GTCTCTCTTGTTGAAGAGTA+TGG | 0.345465 | 8.1:-15253388 | MS.gene68975:CDS |
| CTGTCAAATGCAAGAGAATC+AGG | 0.348024 | 8.1:+15254669 | None:intergenic |
| AATAGTCTTACTTTGTCTTC+GGG | 0.365443 | 8.1:-15253448 | MS.gene68975:CDS |
| GATTTATTCATGCTACTTGC+TGG | 0.370329 | 8.1:-15253523 | MS.gene68975:CDS |
| CTGTAAAACTATACCATTCT+CGG | 0.373836 | 8.1:+15253414 | None:intergenic |
| ATGCAGCTGATGCTTATTAT+GGG | 0.375296 | 8.1:-15254307 | MS.gene68975:CDS |
| ATCAGCAAACACGGTAGAAT+TGG | 0.379198 | 8.1:+15254226 | None:intergenic |
| AATAAACGATTCCAGTGTTT+GGG | 0.380124 | 8.1:+15254189 | None:intergenic |
| GCAATATGTGGAAGGCCATT+AGG | 0.400762 | 8.1:-15254362 | MS.gene68975:CDS |
| CACTGGTTAGCAGCGCTGTC+TGG | 0.406496 | 8.1:+15253737 | None:intergenic |
| TGCTTGGATCATAACTCAAT+AGG | 0.410469 | 8.1:+15253692 | None:intergenic |
| ACTGTTGTCTGGTTGTTGCT+TGG | 0.420207 | 8.1:+15253676 | None:intergenic |
| GATTTATATATTTACCTCTT+GGG | 0.420261 | 8.1:+15254552 | None:intergenic |
| TTGTGCAGGTCCAACTAATT+GGG | 0.425364 | 8.1:+15254250 | None:intergenic |
| TTCTCAGTAACACTGTTGTC+TGG | 0.431970 | 8.1:+15253665 | None:intergenic |
| GAGTACAATGGAACACTCTA+TGG | 0.432828 | 8.1:-15253337 | MS.gene68975:CDS |
| TGTGGAAGGCCATTAGGATT+GGG | 0.435505 | 8.1:-15254356 | MS.gene68975:CDS |
| TTGGGTTTCAATCATCAAAC+AGG | 0.444949 | 8.1:-15254338 | MS.gene68975:CDS |
| AGAGTATGGTCTTCTTGAAC+CGG | 0.453051 | 8.1:-15253374 | MS.gene68975:CDS |
| ATGGCCTTCCACATATTGCT+TGG | 0.453479 | 8.1:+15254366 | None:intergenic |
| CTTGTAAAGGTTGGCCCCAA+TGG | 0.461054 | 8.1:-15254284 | MS.gene68975:CDS |
| ATGTGGAAGGCCATTAGGAT+TGG | 0.461713 | 8.1:-15254357 | MS.gene68975:CDS |
| AAAACTATGCTTACTTGAGC+TGG | 0.464925 | 8.1:+15254144 | None:intergenic |
| GAAATCTTAAATTTGCATCT+GGG | 0.465666 | 8.1:-15253645 | MS.gene68975:CDS |
| TGGTCCAAGTGAAGGTTTCA+AGG | 0.466762 | 8.1:-15254592 | MS.gene68975:CDS |
| TATTATGGGCTTGTAAAGGT+TGG | 0.470412 | 8.1:-15254293 | MS.gene68975:CDS |
| CAGTAGAAAGATAGCAAGAA+TGG | 0.470940 | 8.1:+15254755 | None:intergenic |
| AGTGGCATTGGAGATAATTC+AGG | 0.481306 | 8.1:-15253718 | MS.gene68975:CDS |
| TACTCTTGAACCTCACTAGC+CGG | 0.499543 | 8.1:+15253355 | None:intergenic |
| GGGAAAGCAACCCAATTAGT+TGG | 0.500291 | 8.1:-15254260 | MS.gene68975:CDS |
| GCGCTGCTAACCAGTGGCAT+TGG | 0.502363 | 8.1:-15253730 | MS.gene68975:CDS |
| TGATTGAAACCCAATCCTAA+TGG | 0.505270 | 8.1:+15254347 | None:intergenic |
| TCTCTTGCATTTGACAGAAA+CGG | 0.511849 | 8.1:-15254663 | MS.gene68975:CDS |
| AATTCTACCGTGTTTGCTGA+TGG | 0.517101 | 8.1:-15254224 | MS.gene68975:CDS |
| TGAATTATCTCCAATGCCAC+TGG | 0.518510 | 8.1:+15253720 | None:intergenic |
| CTTGTTCAGTAATATGGCTA+TGG | 0.523436 | 8.1:+15254719 | None:intergenic |
| CCATCAGAAGAAGTAACATA+AGG | 0.525792 | 8.1:+15254633 | None:intergenic |
| CCTTATGTTACTTCTTCTGA+TGG | 0.527626 | 8.1:-15254633 | MS.gene68975:CDS |
| AAATATGTTGGTCCAAGTGA+AGG | 0.529246 | 8.1:-15254600 | MS.gene68975:CDS |
| GGTGTTAGAGTGACCGAGAA+TGG | 0.530351 | 8.1:-15253427 | MS.gene68975:CDS |
| TCAGAAATCCAAGCAATATG+TGG | 0.532707 | 8.1:-15254374 | MS.gene68975:CDS |
| TGCTTATTATGGGCTTGTAA+AGG | 0.537666 | 8.1:-15254297 | MS.gene68975:CDS |
| GGGGTGGCAGTAAGCGGAGA+TGG | 0.543707 | 8.1:-15253625 | MS.gene68975:CDS |
| ACGGTAGAATTGGCTTGTGC+AGG | 0.544655 | 8.1:+15254236 | None:intergenic |
| TCAGGTCCTTTCACTGGTGA+TGG | 0.547336 | 8.1:+15254687 | None:intergenic |
| ATTCGGAGAGTGTGGTTGAA+AGG | 0.547509 | 8.1:-15253565 | MS.gene68975:CDS |
| TGATTGCATAAGAAGCAAGC+AGG | 0.550700 | 8.1:+15253308 | None:intergenic |
| GAAGACCAGATAACATAAAG+AGG | 0.552163 | 8.1:-15253501 | MS.gene68975:CDS |
| AGAGAATCAGGTCCTTTCAC+TGG | 0.553043 | 8.1:+15254681 | None:intergenic |
| TTAGATGTGGACCCAAACAC+TGG | 0.559091 | 8.1:-15254200 | MS.gene68975:CDS |
| AAATCTTAAATTTGCATCTG+GGG | 0.560132 | 8.1:-15253644 | MS.gene68975:CDS |
| GTAAAGGTTGGCCCCAATGG+AGG | 0.566207 | 8.1:-15254281 | MS.gene68975:CDS |
| TAAAGGTTGGCCCCAATGGA+GGG | 0.566544 | 8.1:-15254280 | MS.gene68975:CDS |
| TATTCCTTGAAACCTTCACT+TGG | 0.569604 | 8.1:+15254588 | None:intergenic |
| CATTAATAATTCTATGGTGA+TGG | 0.573040 | 8.1:-15254787 | MS.gene68975:CDS |
| TCTTCTTGAACCGGCTAGTG+AGG | 0.578667 | 8.1:-15253365 | MS.gene68975:CDS |
| CTAACACAATTCGGAGAGTG+TGG | 0.584118 | 8.1:-15253573 | MS.gene68975:CDS |
| GTTTGCTGATGGTTTAGATG+TGG | 0.585669 | 8.1:-15254213 | MS.gene68975:CDS |
| GGGTTGCTTTCCCTCCATTG+GGG | 0.591391 | 8.1:+15254270 | None:intergenic |
| CTGGAATTTAGTGCTAGCTA+TGG | 0.599531 | 8.1:+15254163 | None:intergenic |
| AGGAACAGGACAATCTGTGA+TGG | 0.601201 | 8.1:-15254410 | MS.gene68975:CDS |
| ATCTAAACCATCAGCAAACA+CGG | 0.601997 | 8.1:+15254217 | None:intergenic |
| GCATCTGGGGTGGCAGTAAG+CGG | 0.604290 | 8.1:-15253631 | MS.gene68975:CDS |
| TCTTAAATTTGCATCTGGGG+TGG | 0.610328 | 8.1:-15253641 | MS.gene68975:CDS |
| AAATCCAAGCAATATGTGGA+AGG | 0.615490 | 8.1:-15254370 | MS.gene68975:CDS |
| GCATTTGACAGAAACGGTGA+AGG | 0.616080 | 8.1:-15254657 | MS.gene68975:CDS |
| CAGTTACCATCACCAGTGAA+AGG | 0.618750 | 8.1:-15254693 | MS.gene68975:CDS |
| AACATAAAGAGGAATTCAAG+AGG | 0.628213 | 8.1:-15253490 | MS.gene68975:CDS |
| GATTGCATAAGAAGCAAGCA+GGG | 0.638280 | 8.1:+15253309 | None:intergenic |
| TACAATGGAACACTCTATGG+TGG | 0.656288 | 8.1:-15253334 | MS.gene68975:CDS |
| AAGTAGCATGAATAAATCTG+AGG | 0.664600 | 8.1:+15253528 | None:intergenic |
| CAGACAGCGCTGCTAACCAG+TGG | 0.665038 | 8.1:-15253736 | MS.gene68975:CDS |
| ATGCTTACACTTCTCCCAAG+AGG | 0.667804 | 8.1:-15254566 | MS.gene68975:intron |
| AGTGAGGTTCAAGAGTACAA+TGG | 0.673039 | 8.1:-15253349 | MS.gene68975:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAATTACTATGTAAA+TGG | + | chr8.1:15253598-15253617 | None:intergenic | 10.0% |
| !!! | AATGATTGTTTTTTATTTTT+TGG | - | chr8.1:15253653-15253672 | MS.gene68975:CDS | 10.0% |
| !! | TGATAATTTATGAGAAAAAT+TGG | + | chr8.1:15254004-15254023 | None:intergenic | 15.0% |
| !! | TTCAATTATGTTATTGTTTA+TGG | - | chr8.1:15254222-15254241 | MS.gene68975:CDS | 15.0% |
| !! | GATTTATATATTTACCTCTT+GGG | + | chr8.1:15253540-15253559 | None:intergenic | 20.0% |
| !! | GTTGAACATTAATAATTCTA+TGG | - | chr8.1:15253296-15253315 | MS.gene68975:CDS | 20.0% |
| !! | TGATTTATATATTTACCTCT+TGG | + | chr8.1:15253541-15253560 | None:intergenic | 20.0% |
| !!! | AATATTTTGCTAACACAATT+CGG | - | chr8.1:15254507-15254526 | MS.gene68975:intron | 20.0% |
| !!! | TGTTTTTTATTTTTTGGTTC+AGG | - | chr8.1:15253659-15253678 | MS.gene68975:CDS | 20.0% |
| ! | AAATCTTAAATTTGCATCTG+GGG | - | chr8.1:15254445-15254464 | MS.gene68975:intron | 25.0% |
| ! | AGAAATCTTAAATTTGCATC+TGG | - | chr8.1:15254443-15254462 | MS.gene68975:intron | 25.0% |
| ! | CATTAATAATTCTATGGTGA+TGG | - | chr8.1:15253302-15253321 | MS.gene68975:CDS | 25.0% |
| ! | CTTTCTTTCCTCAAATAATT+TGG | - | chr8.1:15254195-15254214 | MS.gene68975:CDS | 25.0% |
| ! | GAAATCTTAAATTTGCATCT+GGG | - | chr8.1:15254444-15254463 | MS.gene68975:intron | 25.0% |
| ! | TATTAACTGCAATCCAAAAT+TGG | + | chr8.1:15254625-15254644 | None:intergenic | 25.0% |
| !! | GGAAGAATTTTCAAATATGT+TGG | - | chr8.1:15253477-15253496 | MS.gene68975:CDS | 25.0% |
| !! | GTTTAGTTTCAAAATCATAG+AGG | - | chr8.1:15254259-15254278 | MS.gene68975:CDS | 25.0% |
| !! | TAATAGTCTTACTTTGTCTT+CGG | - | chr8.1:15254640-15254659 | MS.gene68975:CDS | 25.0% |
| AAATAAACGATTCCAGTGTT+TGG | + | chr8.1:15253904-15253923 | None:intergenic | 30.0% | |
| AACATAAAGAGGAATTCAAG+AGG | - | chr8.1:15254599-15254618 | MS.gene68975:CDS | 30.0% | |
| AAGTAGCATGAATAAATCTG+AGG | + | chr8.1:15254564-15254583 | None:intergenic | 30.0% | |
| AATAAACGATTCCAGTGTTT+GGG | + | chr8.1:15253903-15253922 | None:intergenic | 30.0% | |
| CTGTAAAACTATACCATTCT+CGG | + | chr8.1:15254678-15254697 | None:intergenic | 30.0% | |
| CTTTCCTCAAATAATTTGGA+GGG | - | chr8.1:15254199-15254218 | MS.gene68975:CDS | 30.0% | |
| GAATTCCTCTTTATGTTATC+TGG | + | chr8.1:15254596-15254615 | None:intergenic | 30.0% | |
| TCTTTCCTCAAATAATTTGG+AGG | - | chr8.1:15254198-15254217 | MS.gene68975:CDS | 30.0% | |
| ! | AATAGTCTTACTTTGTCTTC+GGG | - | chr8.1:15254641-15254660 | MS.gene68975:CDS | 30.0% |
| !! | AAATCTGAGGAATTTGCTTT+TGG | + | chr8.1:15254551-15254570 | None:intergenic | 30.0% |
| !!! | TTATTTTTTGGTTCAGGAAC+AGG | - | chr8.1:15253665-15253684 | MS.gene68975:CDS | 30.0% |
| AAAACTATGCTTACTTGAGC+TGG | + | chr8.1:15253948-15253967 | None:intergenic | 35.0% | |
| AAATATGTTGGTCCAAGTGA+AGG | - | chr8.1:15253489-15253508 | MS.gene68975:CDS | 35.0% | |
| AAATCCAAGCAATATGTGGA+AGG | - | chr8.1:15253719-15253738 | MS.gene68975:CDS | 35.0% | |
| ATCTAAACCATCAGCAAACA+CGG | + | chr8.1:15253875-15253894 | None:intergenic | 35.0% | |
| ATGCAGCTGATGCTTATTAT+GGG | - | chr8.1:15253782-15253801 | MS.gene68975:intron | 35.0% | |
| ATGGTCACTTGATTCATGTT+AGG | + | chr8.1:15253579-15253598 | None:intergenic | 35.0% | |
| CAGTAGAAAGATAGCAAGAA+TGG | + | chr8.1:15253337-15253356 | None:intergenic | 35.0% | |
| CCATCAGAAGAAGTAACATA+AGG | + | chr8.1:15253459-15253478 | None:intergenic | 35.0% | |
| CTTGTTCAGTAATATGGCTA+TGG | + | chr8.1:15253373-15253392 | None:intergenic | 35.0% | |
| GAAGACCAGATAACATAAAG+AGG | - | chr8.1:15254588-15254607 | MS.gene68975:CDS | 35.0% | |
| TATGCAGCTGATGCTTATTA+TGG | - | chr8.1:15253781-15253800 | MS.gene68975:intron | 35.0% | |
| TATTATGGGCTTGTAAAGGT+TGG | - | chr8.1:15253796-15253815 | MS.gene68975:intron | 35.0% | |
| TATTCCTTGAAACCTTCACT+TGG | + | chr8.1:15253504-15253523 | None:intergenic | 35.0% | |
| TCAGAAATCCAAGCAATATG+TGG | - | chr8.1:15253715-15253734 | MS.gene68975:CDS | 35.0% | |
| TCTCTTGCATTTGACAGAAA+CGG | - | chr8.1:15253426-15253445 | MS.gene68975:CDS | 35.0% | |
| TGAACCCTCCAAATTATTTG+AGG | + | chr8.1:15254206-15254225 | None:intergenic | 35.0% | |
| TGATTGAAACCCAATCCTAA+TGG | + | chr8.1:15253745-15253764 | None:intergenic | 35.0% | |
| TGCTTATTATGGGCTTGTAA+AGG | - | chr8.1:15253792-15253811 | MS.gene68975:intron | 35.0% | |
| TGCTTGGATCATAACTCAAT+AGG | + | chr8.1:15254400-15254419 | None:intergenic | 35.0% | |
| TGGTCACTTGATTCATGTTA+GGG | + | chr8.1:15253578-15253597 | None:intergenic | 35.0% | |
| TTGGGTTTCAATCATCAAAC+AGG | - | chr8.1:15253751-15253770 | MS.gene68975:intron | 35.0% | |
| ! | CCTTATGTTACTTCTTCTGA+TGG | - | chr8.1:15253456-15253475 | MS.gene68975:CDS | 35.0% |
| ! | CTGAAGCTTGTTCAGTAATA+TGG | + | chr8.1:15253379-15253398 | None:intergenic | 35.0% |
| ! | GATTTATTCATGCTACTTGC+TGG | - | chr8.1:15254566-15254585 | MS.gene68975:intron | 35.0% |
| AGAGTATGGTCTTCTTGAAC+CGG | - | chr8.1:15254715-15254734 | MS.gene68975:CDS | 40.0% | |
| ATCAGCAAACACGGTAGAAT+TGG | + | chr8.1:15253866-15253885 | None:intergenic | 40.0% | |
| CTGGAATTTAGTGCTAGCTA+TGG | + | chr8.1:15253929-15253948 | None:intergenic | 40.0% | |
| CTGTCAAATGCAAGAGAATC+AGG | + | chr8.1:15253423-15253442 | None:intergenic | 40.0% | |
| GAGTACAATGGAACACTCTA+TGG | - | chr8.1:15254752-15254771 | MS.gene68975:CDS | 40.0% | |
| GATTGCATAAGAAGCAAGCA+GGG | + | chr8.1:15254783-15254802 | None:intergenic | 40.0% | |
| GTCTCTCTTGTTGAAGAGTA+TGG | - | chr8.1:15254701-15254720 | MS.gene68975:CDS | 40.0% | |
| TACAATGGAACACTCTATGG+TGG | - | chr8.1:15254755-15254774 | MS.gene68975:CDS | 40.0% | |
| TCTTAAATTTGCATCTGGGG+TGG | - | chr8.1:15254448-15254467 | MS.gene68975:intron | 40.0% | |
| TGAATTATCTCCAATGCCAC+TGG | + | chr8.1:15254372-15254391 | None:intergenic | 40.0% | |
| TGATTGCATAAGAAGCAAGC+AGG | + | chr8.1:15254784-15254803 | None:intergenic | 40.0% | |
| TTCTCAGTAACACTGTTGTC+TGG | + | chr8.1:15254427-15254446 | None:intergenic | 40.0% | |
| TTGTGCAGGTCCAACTAATT+GGG | + | chr8.1:15253842-15253861 | None:intergenic | 40.0% | |
| ! | AATTCTACCGTGTTTGCTGA+TGG | - | chr8.1:15253865-15253884 | MS.gene68975:intron | 40.0% |
| ! | AGTGAGGTTCAAGAGTACAA+TGG | - | chr8.1:15254740-15254759 | MS.gene68975:CDS | 40.0% |
| ! | AGTGGCATTGGAGATAATTC+AGG | - | chr8.1:15254371-15254390 | MS.gene68975:CDS | 40.0% |
| ! | GGAATTCAAGAGGCCAATTT+TGG | - | chr8.1:15254609-15254628 | MS.gene68975:CDS | 40.0% |
| ! | GTTTGCTGATGGTTTAGATG+TGG | - | chr8.1:15253876-15253895 | MS.gene68975:intron | 40.0% |
| ACTGTTGTCTGGTTGTTGCT+TGG | + | chr8.1:15254416-15254435 | None:intergenic | 45.0% | |
| AGAGAATCAGGTCCTTTCAC+TGG | + | chr8.1:15253411-15253430 | None:intergenic | 45.0% | |
| AGCATGCGTCACAGATGTTA+GGG | + | chr8.1:15254161-15254180 | None:intergenic | 45.0% | |
| AGGAACAGGACAATCTGTGA+TGG | - | chr8.1:15253679-15253698 | MS.gene68975:CDS | 45.0% | |
| ATGCTTACACTTCTCCCAAG+AGG | - | chr8.1:15253523-15253542 | MS.gene68975:CDS | 45.0% | |
| ATGGCCTTCCACATATTGCT+TGG | + | chr8.1:15253726-15253745 | None:intergenic | 45.0% | |
| ATGTGGAAGGCCATTAGGAT+TGG | - | chr8.1:15253732-15253751 | MS.gene68975:CDS | 45.0% | |
| CAGTTACCATCACCAGTGAA+AGG | - | chr8.1:15253396-15253415 | MS.gene68975:CDS | 45.0% | |
| CTAACACAATTCGGAGAGTG+TGG | - | chr8.1:15254516-15254535 | MS.gene68975:intron | 45.0% | |
| CTTGTGCAGGTCCAACTAAT+TGG | + | chr8.1:15253843-15253862 | None:intergenic | 45.0% | |
| GCAATATGTGGAAGGCCATT+AGG | - | chr8.1:15253727-15253746 | MS.gene68975:CDS | 45.0% | |
| GCATTTGACAGAAACGGTGA+AGG | - | chr8.1:15253432-15253451 | MS.gene68975:CDS | 45.0% | |
| GGGAAAGCAACCCAATTAGT+TGG | - | chr8.1:15253829-15253848 | MS.gene68975:intron | 45.0% | |
| TACTCTTGAACCTCACTAGC+CGG | + | chr8.1:15254737-15254756 | None:intergenic | 45.0% | |
| TGGTCCAAGTGAAGGTTTCA+AGG | - | chr8.1:15253497-15253516 | MS.gene68975:CDS | 45.0% | |
| TGTGGAAGGCCATTAGGATT+GGG | - | chr8.1:15253733-15253752 | MS.gene68975:CDS | 45.0% | |
| TTAGATGTGGACCCAAACAC+TGG | - | chr8.1:15253889-15253908 | MS.gene68975:intron | 45.0% | |
| ! | ATTCGGAGAGTGTGGTTGAA+AGG | - | chr8.1:15254524-15254543 | MS.gene68975:intron | 45.0% |
| GAGCATGCGTCACAGATGTT+AGG | + | chr8.1:15254162-15254181 | None:intergenic | 50.0% | |
| GCATGCGTCACAGATGTTAG+GGG | + | chr8.1:15254160-15254179 | None:intergenic | 50.0% | |
| GGTGTTAGAGTGACCGAGAA+TGG | - | chr8.1:15254662-15254681 | MS.gene68975:CDS | 50.0% | |
| TCAGGTCCTTTCACTGGTGA+TGG | + | chr8.1:15253405-15253424 | None:intergenic | 50.0% | |
| TCTTCTTGAACCGGCTAGTG+AGG | - | chr8.1:15254724-15254743 | MS.gene68975:CDS | 50.0% | |
| ! | CTTGTAAAGGTTGGCCCCAA+TGG | - | chr8.1:15253805-15253824 | MS.gene68975:intron | 50.0% |
| ! | TAAAGGTTGGCCCCAATGGA+GGG | - | chr8.1:15253809-15253828 | MS.gene68975:intron | 50.0% |
| ! | TGGGTTGCTTTCCCTCCATT+GGG | + | chr8.1:15253823-15253842 | None:intergenic | 50.0% |
| ! | TTGGGTTGCTTTCCCTCCAT+TGG | + | chr8.1:15253824-15253843 | None:intergenic | 50.0% |
| !! | ACGGTAGAATTGGCTTGTGC+AGG | + | chr8.1:15253856-15253875 | None:intergenic | 50.0% |
| ! | GGGTTGCTTTCCCTCCATTG+GGG | + | chr8.1:15253822-15253841 | None:intergenic | 55.0% |
| ! | GTAAAGGTTGGCCCCAATGG+AGG | - | chr8.1:15253808-15253827 | MS.gene68975:intron | 55.0% |
| CAGACAGCGCTGCTAACCAG+TGG | - | chr8.1:15254353-15254372 | MS.gene68975:CDS | 60.0% | |
| GCGCTGCTAACCAGTGGCAT+TGG | - | chr8.1:15254359-15254378 | MS.gene68975:CDS | 60.0% | |
| ! | CACTGGTTAGCAGCGCTGTC+TGG | + | chr8.1:15254355-15254374 | None:intergenic | 60.0% |
| ! | GCATCTGGGGTGGCAGTAAG+CGG | - | chr8.1:15254458-15254477 | MS.gene68975:intron | 60.0% |
| GGGGTGGCAGTAAGCGGAGA+TGG | - | chr8.1:15254464-15254483 | MS.gene68975:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 15253294 | 15254817 | 15253294 | ID=MS.gene68975 |
| chr8.1 | mRNA | 15253294 | 15254817 | 15253294 | ID=MS.gene68975.t1;Parent=MS.gene68975 |
| chr8.1 | exon | 15254567 | 15254817 | 15254567 | ID=MS.gene68975.t1.exon1;Parent=MS.gene68975.t1 |
| chr8.1 | CDS | 15254567 | 15254817 | 15254567 | ID=cds.MS.gene68975.t1;Parent=MS.gene68975.t1 |
| chr8.1 | exon | 15254158 | 15254430 | 15254158 | ID=MS.gene68975.t1.exon2;Parent=MS.gene68975.t1 |
| chr8.1 | CDS | 15254158 | 15254430 | 15254158 | ID=cds.MS.gene68975.t1;Parent=MS.gene68975.t1 |
| chr8.1 | exon | 15253294 | 15253765 | 15253294 | ID=MS.gene68975.t1.exon3;Parent=MS.gene68975.t1 |
| chr8.1 | CDS | 15253294 | 15253765 | 15253294 | ID=cds.MS.gene68975.t1;Parent=MS.gene68975.t1 |
| Gene Sequence |
| Protein sequence |
