AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83236

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83236.t1 MTR_2g058450 79.525 337 67 2 1 336 1 336 1.25e-175 490
MS.gene83236.t1 MTR_2g058410 95.690 232 10 0 104 335 1 232 3.59e-151 424
MS.gene83236.t1 MTR_4g112650 54.762 336 149 2 1 335 1 334 7.02e-121 351
MS.gene83236.t1 MTR_4g112670 53.916 332 148 3 5 334 6 334 5.86e-116 339
MS.gene83236.t1 MTR_4g112630 52.819 337 145 3 1 335 1 325 6.84e-111 326
MS.gene83236.t1 MTR_4g112620 48.503 334 133 3 2 335 6 300 2.49e-99 295
MS.gene83236.t1 MTR_7g490330 40.717 307 162 4 31 336 38 325 4.30e-70 221
MS.gene83236.t1 MTR_7g490350 37.500 320 168 5 40 333 53 366 8.34e-65 209
MS.gene83236.t1 MTR_5g091980 40.584 308 160 5 30 336 38 323 1.60e-63 204
MS.gene83236.t1 MTR_7g090320 37.725 334 157 7 40 331 58 382 3.73e-63 206
MS.gene83236.t1 MTR_7g090310 37.805 328 164 8 40 331 58 381 4.08e-62 203
MS.gene83236.t1 MTR_7g090300 37.888 322 170 6 40 336 53 369 1.97e-61 201
MS.gene83236.t1 MTR_4g050190 36.890 328 170 9 37 333 67 388 7.91e-60 197
MS.gene83236.t1 MTR_2g087780 36.795 337 177 8 28 333 61 392 1.12e-58 194
MS.gene83236.t1 MTR_0003s0530 55.249 181 78 2 148 326 2 181 1.31e-58 188
MS.gene83236.t1 MTR_2g099030 31.751 337 198 5 28 334 70 404 8.55e-51 174
MS.gene83236.t1 MTR_2g058430 88.095 84 10 0 93 176 50 133 1.34e-46 159
MS.gene83236.t1 MTR_8g074210 32.773 238 127 6 40 273 78 286 1.40e-30 119
MS.gene83236.t1 MTR_4g112840 52.475 101 47 1 174 274 56 155 7.26e-28 107
MS.gene83236.t1 MTR_8g074270 31.513 238 136 6 37 273 75 286 4.49e-27 110
MS.gene83236.t1 MTR_8g074170 31.950 241 131 7 37 273 75 286 2.36e-25 105
MS.gene83236.t1 MTR_2g042430 32.051 234 129 10 38 269 74 279 2.38e-19 88.2
MS.gene83236.t1 MTR_2g042370 34.395 157 97 5 115 269 116 268 7.57e-18 83.6
MS.gene83236.t1 MTR_2g042420 33.121 157 98 5 115 269 109 260 2.08e-16 79.3
MS.gene83236.t1 MTR_8g074320 35.811 148 79 6 85 225 81 219 5.06e-16 76.3
MS.gene83236.t1 MTR_5g077620 48.810 84 40 1 7 90 4 84 8.48e-16 73.9
MS.gene83236.t1 MTR_1g075865 38.318 107 62 3 152 257 7 110 4.97e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83236.t1 AT1G74020 43.087 311 160 8 29 334 27 325 4.85e-75 234
MS.gene83236.t1 AT1G74000 41.748 309 167 6 29 334 29 327 1.96e-74 233
MS.gene83236.t1 AT1G74010 42.444 311 165 7 29 336 26 325 1.47e-70 223
MS.gene83236.t1 AT3G57030 40.000 315 157 7 39 326 55 364 2.06e-63 206
MS.gene83236.t1 AT5G22020 37.117 326 171 9 37 333 72 392 3.85e-63 206
MS.gene83236.t1 AT5G22020 37.117 326 171 9 37 333 72 392 3.85e-63 206
MS.gene83236.t1 AT5G22020 37.117 326 171 9 37 333 72 392 3.85e-63 206
MS.gene83236.t1 AT1G08470 36.810 326 172 9 37 333 66 386 4.13e-58 192
MS.gene83236.t1 AT2G41290 36.283 339 179 8 31 336 42 376 6.39e-56 186
MS.gene83236.t1 AT3G57010 39.344 244 139 4 28 270 42 277 1.89e-53 180
MS.gene83236.t1 AT3G57020 37.553 237 141 2 34 270 47 276 5.38e-50 171
MS.gene83236.t1 AT3G59530 37.553 237 139 4 40 270 84 317 2.83e-47 164
MS.gene83236.t1 AT3G59530 37.553 237 139 4 40 270 84 317 2.83e-47 164
MS.gene83236.t1 AT3G59530 37.553 237 139 4 40 270 84 317 2.83e-47 164
MS.gene83236.t1 AT2G41300 37.288 236 141 2 35 270 73 301 2.85e-46 162
MS.gene83236.t1 AT3G57020 35.021 237 133 2 34 270 47 262 8.29e-44 154
MS.gene83236.t1 AT3G51440 29.536 237 135 8 38 269 67 276 4.90e-22 95.9
MS.gene83236.t1 AT3G51430 28.270 237 138 8 38 269 67 276 1.64e-20 91.7
MS.gene83236.t1 AT3G51430 28.270 237 138 8 38 269 67 276 1.71e-20 91.7
MS.gene83236.t1 AT3G51450 26.180 233 148 7 38 269 67 276 6.71e-19 87.0

Find 91 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCGGCTATTAAGCAGTTTC+TGG 0.156926 2.4:-34340919 None:intergenic
ATGTACTAGGCAGCCCTTTC+GGG 0.172090 2.4:+34340360 MS.gene83236:CDS
TATATAGCTGATGCTTATTA+TGG 0.199171 2.4:+34340287 MS.gene83236:CDS
GGACCTCGAGGAACGAGTTT+TGG 0.237051 2.4:+34341170 MS.gene83236:CDS
AATGTGGTTTGTTTCGTTAT+TGG 0.242281 2.4:-34340974 None:intergenic
CGAATAGAATCCGGAGATTC+TGG 0.258519 2.4:+34341077 MS.gene83236:CDS
ATCCGGAGATTCTGGCTTAC+AGG 0.284143 2.4:+34341085 MS.gene83236:CDS
TAGATGATGAATAGAGTATC+AGG 0.288393 2.4:-34339744 None:intergenic
GTGCCTCTTGTTGAGGAGTT+TGG 0.291880 2.4:+34341292 MS.gene83236:CDS
TAGGCCTCCCACATGTTTCT+TGG 0.297657 2.4:-34340229 None:intergenic
GTGGCAGTGAATTATCCTTT+TGG 0.303939 2.4:+34341193 MS.gene83236:CDS
GATATATTCTTGTGGCTTCC+AGG 0.310786 2.4:+34341127 MS.gene83236:CDS
AGCCCTTTCGGGTTTCTTGC+TGG 0.336808 2.4:+34340371 MS.gene83236:CDS
AATGTACTAGGCAGCCCTTT+CGG 0.343008 2.4:+34340359 MS.gene83236:CDS
CTTGTTGAGGAGTTTGGCAC+TGG 0.354664 2.4:+34341298 MS.gene83236:CDS
CGTAATCTAGCTATGGCAAC+AGG 0.355591 2.4:+34341004 MS.gene83236:CDS
TTATTGGATTGTATCTGAAC+AGG 0.380994 2.4:-34340958 None:intergenic
AGCAACATGGCTGTTATCAC+TGG 0.386843 2.4:+34339709 MS.gene83236:CDS
CTTGTGAAGGTTTCTTATGA+TGG 0.407212 2.4:+34340311 MS.gene83236:CDS
CAACTTGTTTCTAATGTACT+AGG 0.408485 2.4:+34340347 MS.gene83236:CDS
TATCTTCCACCATCACTAAC+AGG 0.409455 2.4:+34339799 MS.gene83236:CDS
AAATAAACGATTCCAGTGTT+AGG 0.413431 2.4:-34340407 None:intergenic
TCCCTAGCATTTGATTCCAT+TGG 0.419739 2.4:+34339829 MS.gene83236:CDS
CACTGGAATCGTTTATTTCA+CGG 0.419917 2.4:+34340412 MS.gene83236:CDS
CTAGCATTTGATTCCATTGG+TGG 0.422268 2.4:+34339832 MS.gene83236:CDS
TCTGTCTAGTAATTTGCAAT+AGG 0.427239 2.4:-34339773 None:intergenic
ATATCTGAAGTATATGCATT+TGG 0.445300 2.4:-34341109 None:intergenic
CTTAGTCCTAGAGGCAATAC+AGG 0.446065 2.4:-34341238 None:intergenic
CTAGCTATTATCACAAGATC+AGG 0.448677 2.4:+34340438 MS.gene83236:CDS
AGAAACATGTGGGAGGCCTA+TGG 0.451046 2.4:+34340232 MS.gene83236:CDS
GAATCAAATGCTAGGGACTC+AGG 0.459141 2.4:-34339823 None:intergenic
GCATTTGATTCCATTGGTGG+AGG 0.460168 2.4:+34339835 MS.gene83236:CDS
CTGTTGCCATAGCTAGATTA+CGG 0.466820 2.4:-34341003 None:intergenic
GTCCTGTAAGCCAGAATCTC+CGG 0.467572 2.4:-34341087 None:intergenic
GAAACATGTGGGAGGCCTAT+GGG 0.483364 2.4:+34340233 MS.gene83236:CDS
CCTTACACTGGTGTTTCTGA+TGG 0.486655 2.4:+34339859 MS.gene83236:CDS
AGATTAGACACGTACCGGTC+TGG 0.488853 2.4:-34339940 None:intergenic
AGAGGCTGTGCCTCTTGTTG+AGG 0.494455 2.4:+34341285 MS.gene83236:CDS
TTGATGTTATCTGGTCTCCC+TGG 0.500478 2.4:-34341145 None:intergenic
TTTCGGGTTTCTTGCTGGCG+TGG 0.506089 2.4:+34340376 MS.gene83236:CDS
AGAGGCAATACAGGAGGTAC+TGG 0.506539 2.4:-34341229 None:intergenic
CCATCAGAAACACCAGTGTA+AGG 0.510401 2.4:-34339859 None:intergenic
GACTCAGGGCCTGTTAGTGA+TGG 0.512435 2.4:-34339808 None:intergenic
AGTACAAGGCGAATAGAATC+CGG 0.518601 2.4:+34341068 MS.gene83236:CDS
ATTGGTGGAGGGCCTTACAC+TGG 0.525491 2.4:+34339847 MS.gene83236:CDS
CATTTGATTCCATTGGTGGA+GGG 0.528926 2.4:+34339836 MS.gene83236:CDS
GGAGACCAGATAACATCAAA+AGG 0.530015 2.4:+34341149 MS.gene83236:CDS
ATATATTCTTGTGGCTTCCA+GGG 0.532891 2.4:+34341128 MS.gene83236:CDS
AGGAACAAAACAATTTGTGA+TGG 0.538253 2.4:+34340185 MS.gene83236:intron
AGTGTAAGGCCCTCCACCAA+TGG 0.538991 2.4:-34339845 None:intergenic
TCGCCTTGTACTCACTTACA+AGG 0.539423 2.4:-34341057 None:intergenic
TCAGGGCCTGTTAGTGATGG+TGG 0.541903 2.4:-34339805 None:intergenic
CGCCAGCAAGAAACCCGAAA+GGG 0.545590 2.4:-34340373 None:intergenic
TGTCCTTGTAAGTGAGTACA+AGG 0.546556 2.4:+34341054 MS.gene83236:CDS
AATACAGGAGGTACTGGTGG+TGG 0.552721 2.4:-34341223 None:intergenic
TTGCTCACATTTCACCAGAC+CGG 0.560560 2.4:+34339926 MS.gene83236:CDS
TGCTTATTATGGACTTGTGA+AGG 0.563222 2.4:+34340298 MS.gene83236:CDS
GTACCTCCTGTATTGCCTCT+AGG 0.564887 2.4:+34341232 MS.gene83236:CDS
ACCAATGGAATCAAATGCTA+GGG 0.565533 2.4:-34339830 None:intergenic
CACCAATGGAATCAAATGCT+AGG 0.576942 2.4:-34339831 None:intergenic
GTTGCTCCGTAATCTAGCTA+TGG 0.579397 2.4:+34340997 MS.gene83236:CDS
ATACTTCAGATATATTCTTG+TGG 0.582130 2.4:+34341119 MS.gene83236:CDS
TCAGAACTCCAAGAAACATG+TGG 0.584132 2.4:+34340221 MS.gene83236:CDS
ACAGGAGGTACTGGTGGTGG+TGG 0.586387 2.4:-34341220 None:intergenic
GGGGTAGCAGTAAGCAGCAA+TGG 0.587063 2.4:+34341025 MS.gene83236:CDS
TCATTGATTCTTAGTCCTAG+AGG 0.592473 2.4:-34341247 None:intergenic
CAGTGAAGTTCATGAAGCTG+AGG 0.595249 2.4:+34341327 MS.gene83236:CDS
GTAATCTAGCTATGGCAACA+GGG 0.595258 2.4:+34341005 MS.gene83236:CDS
AATCAAATGCTAGGGACTCA+GGG 0.595632 2.4:-34339822 None:intergenic
GTGCCAAACTCCTCAACAAG+AGG 0.597643 2.4:-34341295 None:intergenic
TAATTGTAACTCAACCCCAT+AGG 0.600699 2.4:-34340248 None:intergenic
AAACATGTGGGAGGCCTATG+GGG 0.604258 2.4:+34340234 MS.gene83236:CDS
AGATGATGAATAGAGTATCA+GGG 0.607970 2.4:-34339743 None:intergenic
GGACTAAGAATCAATGAGCA+AGG 0.615322 2.4:+34341253 MS.gene83236:CDS
GTGAAATGTGAGCAAACTCA+AGG 0.618650 2.4:-34339918 None:intergenic
GTGAAGGTTTCTTATGATGG+AGG 0.621834 2.4:+34340314 MS.gene83236:CDS
ATTAAACATGTCTAGCAACA+TGG 0.622340 2.4:+34339696 None:intergenic
GGTGGTGGTGGTGACCCAAA+AGG 0.627094 2.4:-34341208 None:intergenic
GCAACATGGCTGTTATCACT+GGG 0.627898 2.4:+34339710 MS.gene83236:CDS
ACGCCAGCAAGAAACCCGAA+AGG 0.628543 2.4:-34340374 None:intergenic
GGCAATACAGGAGGTACTGG+TGG 0.650197 2.4:-34341226 None:intergenic
CAGAACTCCAAGAAACATGT+GGG 0.662175 2.4:+34340222 MS.gene83236:CDS
CACCCAAAACTCGTTCCTCG+AGG 0.665102 2.4:-34341173 None:intergenic
GTGGATGTCGATCCTAACAC+TGG 0.666158 2.4:+34340395 MS.gene83236:CDS
GAGTTACAATTACAAGACGA+AGG 0.667102 2.4:+34340259 MS.gene83236:CDS
AGTGAAGTTCATGAAGCTGA+GGG 0.672665 2.4:+34341328 MS.gene83236:CDS
AACTCCAAGAAACATGTGGG+AGG 0.685654 2.4:+34340225 MS.gene83236:CDS
AGTCCTAGAGGCAATACAGG+AGG 0.688689 2.4:-34341235 None:intergenic
TAGATTACGGAGCAACAATG+TGG 0.690114 2.4:-34340990 None:intergenic
TAATCTAGCTATGGCAACAG+GGG 0.694131 2.4:+34341006 MS.gene83236:CDS
ATAACATCAAAAGGACCTCG+AGG 0.715796 2.4:+34341158 MS.gene83236:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTATTATGTATTATATTT+TGG - chr2.4:34340107-34340126 None:intergenic 10.0%
!! TATGTAATTATAGTCATAGT+TGG + chr2.4:34340133-34340152 MS.gene83236:intron 20.0%
!!! ATTTAGGTCACAATTTTAAT+CGG - chr2.4:34340886-34340905 None:intergenic 20.0%
! AATCATGAGATAGAATAGAA+TGG - chr2.4:34340468-34340487 None:intergenic 25.0%
! ATACTTCAGATATATTCTTG+TGG + chr2.4:34341119-34341138 MS.gene83236:CDS 25.0%
! ATTATAGTCATAGTTGGTTA+AGG + chr2.4:34340139-34340158 MS.gene83236:intron 25.0%
! TATATAGCTGATGCTTATTA+TGG + chr2.4:34340287-34340306 MS.gene83236:CDS 25.0%
! TCTCAAAGCAAGTATATTAA+AGG + chr2.4:34340667-34340686 MS.gene83236:intron 25.0%
!! ATATCTGAAGTATATGCATT+TGG - chr2.4:34341112-34341131 None:intergenic 25.0%
!! CTTTGAGAAGAGAATATTAT+AGG - chr2.4:34340655-34340674 None:intergenic 25.0%
!! TACGTGTCTAATCTAAAATA+TGG + chr2.4:34339949-34339968 MS.gene83236:intron 25.0%
AAATAAACGATTCCAGTGTT+AGG - chr2.4:34340410-34340429 None:intergenic 30.0%
AATGTGAACATCTTTCTTGA+CGG - chr2.4:34339984-34340003 None:intergenic 30.0%
AGGAACAAAACAATTTGTGA+TGG + chr2.4:34340185-34340204 MS.gene83236:intron 30.0%
GTTGCGTTTATAAAGTGTAT+AGG - chr2.4:34340740-34340759 None:intergenic 30.0%
TAGAGCAGATTAAACCTATA+TGG - chr2.4:34340022-34340041 None:intergenic 30.0%
TTATTGGATTGTATCTGAAC+AGG - chr2.4:34340961-34340980 None:intergenic 30.0%
! AATTGTGACCTAAATTTTGC+AGG + chr2.4:34340891-34340910 MS.gene83236:intron 30.0%
! AGATGATGAATAGAGTATCA+GGG - chr2.4:34339746-34339765 None:intergenic 30.0%
! AGTATTGAACTTGACCATAT+AGG + chr2.4:34340005-34340024 MS.gene83236:intron 30.0%
! ATTGTGACCTAAATTTTGCA+GGG + chr2.4:34340892-34340911 MS.gene83236:intron 30.0%
! ATTTTAGATTAGACACGTAC+CGG - chr2.4:34339948-34339967 None:intergenic 30.0%
! TAGATGATGAATAGAGTATC+AGG - chr2.4:34339747-34339766 None:intergenic 30.0%
! TCTGTCTAGTAATTTGCAAT+AGG - chr2.4:34339776-34339795 None:intergenic 30.0%
!! AATGTGGTTTGTTTCGTTAT+TGG - chr2.4:34340977-34340996 None:intergenic 30.0%
!! CAACTTGTTTCTAATGTACT+AGG + chr2.4:34340347-34340366 MS.gene83236:CDS 30.0%
!! TGAGCAAGGTTTAATTTTAG+AGG + chr2.4:34341267-34341286 MS.gene83236:CDS 30.0%
!!! AATAGCCGAGATATTTTTTC+TGG + chr2.4:34340932-34340951 MS.gene83236:CDS 30.0%
ACCAATGGAATCAAATGCTA+GGG - chr2.4:34339833-34339852 None:intergenic 35.0%
ATATATTCTTGTGGCTTCCA+GGG + chr2.4:34341128-34341147 MS.gene83236:CDS 35.0%
CACTGGAATCGTTTATTTCA+CGG + chr2.4:34340412-34340431 MS.gene83236:CDS 35.0%
CATAGCAATGCGAAAAAACA+TGG - chr2.4:34340494-34340513 None:intergenic 35.0%
CTAGCTATTATCACAAGATC+AGG + chr2.4:34340438-34340457 MS.gene83236:CDS 35.0%
CTTGTGAAGGTTTCTTATGA+TGG + chr2.4:34340311-34340330 MS.gene83236:CDS 35.0%
GAGTTACAATTACAAGACGA+AGG + chr2.4:34340259-34340278 MS.gene83236:CDS 35.0%
GCAAATATTCTCTCTAGTCT+TGG + chr2.4:34340860-34340879 MS.gene83236:intron 35.0%
GGCTTCCAGAAAAAATATCT+CGG - chr2.4:34340940-34340959 None:intergenic 35.0%
TAATTGTAACTCAACCCCAT+AGG - chr2.4:34340251-34340270 None:intergenic 35.0%
TGCTTATTATGGACTTGTGA+AGG + chr2.4:34340298-34340317 MS.gene83236:CDS 35.0%
TTCCTGTAACAACAATGACA+TGG - chr2.4:34340170-34340189 None:intergenic 35.0%
! AACCATGTCATTGTTGTTAC+AGG + chr2.4:34340165-34340184 MS.gene83236:intron 35.0%
! TCATGATGACTTGATGATTC+AGG - chr2.4:34340601-34340620 None:intergenic 35.0%
! TGGCAGTGAATTATCCTTTT+GGG + chr2.4:34341194-34341213 MS.gene83236:CDS 35.0%
!! TCATTGATTCTTAGTCCTAG+AGG - chr2.4:34341250-34341269 None:intergenic 35.0%
AATCAAATGCTAGGGACTCA+GGG - chr2.4:34339825-34339844 None:intergenic 40.0%
AGTACAAGGCGAATAGAATC+CGG + chr2.4:34341068-34341087 MS.gene83236:CDS 40.0%
AGTGAAGTTCATGAAGCTGA+GGG + chr2.4:34341328-34341347 MS.gene83236:CDS 40.0%
ATAACATCAAAAGGACCTCG+AGG + chr2.4:34341158-34341177 MS.gene83236:CDS 40.0%
CACCAATGGAATCAAATGCT+AGG - chr2.4:34339834-34339853 None:intergenic 40.0%
CAGAACTCCAAGAAACATGT+GGG + chr2.4:34340222-34340241 MS.gene83236:CDS 40.0%
CATTTGATTCCATTGGTGGA+GGG + chr2.4:34339836-34339855 MS.gene83236:CDS 40.0%
CTAGCATTTGATTCCATTGG+TGG + chr2.4:34339832-34339851 MS.gene83236:CDS 40.0%
CTGTTGCCATAGCTAGATTA+CGG - chr2.4:34341006-34341025 None:intergenic 40.0%
GATATATTCTTGTGGCTTCC+AGG + chr2.4:34341127-34341146 MS.gene83236:CDS 40.0%
GGACTAAGAATCAATGAGCA+AGG + chr2.4:34341253-34341272 MS.gene83236:CDS 40.0%
GGAGACCAGATAACATCAAA+AGG + chr2.4:34341149-34341168 MS.gene83236:CDS 40.0%
GTAATCTAGCTATGGCAACA+GGG + chr2.4:34341005-34341024 MS.gene83236:CDS 40.0%
GTGAAATGTGAGCAAACTCA+AGG - chr2.4:34339921-34339940 None:intergenic 40.0%
GTGAAGGTTTCTTATGATGG+AGG + chr2.4:34340314-34340333 MS.gene83236:CDS 40.0%
TAATCTAGCTATGGCAACAG+GGG + chr2.4:34341006-34341025 MS.gene83236:CDS 40.0%
TAGATTACGGAGCAACAATG+TGG - chr2.4:34340993-34341012 None:intergenic 40.0%
TATCTTCCACCATCACTAAC+AGG + chr2.4:34339799-34339818 MS.gene83236:CDS 40.0%
TCAGAACTCCAAGAAACATG+TGG + chr2.4:34340221-34340240 MS.gene83236:CDS 40.0%
TCCCTAGCATTTGATTCCAT+TGG + chr2.4:34339829-34339848 MS.gene83236:CDS 40.0%
TGGAGATCCCTGCAAAATTT+AGG - chr2.4:34340902-34340921 None:intergenic 40.0%
TGTCCTTGTAAGTGAGTACA+AGG + chr2.4:34341054-34341073 MS.gene83236:CDS 40.0%
! ATGACTTGATGATTCAGGCA+TGG - chr2.4:34340596-34340615 None:intergenic 40.0%
! GAGGTCCTTTTGATGTTATC+TGG - chr2.4:34341157-34341176 None:intergenic 40.0%
! GTGGCAGTGAATTATCCTTT+TGG + chr2.4:34341193-34341212 MS.gene83236:CDS 40.0%
AACTCCAAGAAACATGTGGG+AGG + chr2.4:34340225-34340244 MS.gene83236:CDS 45.0%
AATGTACTAGGCAGCCCTTT+CGG + chr2.4:34340359-34340378 MS.gene83236:CDS 45.0%
AGCAACATGGCTGTTATCAC+TGG + chr2.4:34339709-34339728 MS.gene83236:CDS 45.0%
CAGTGAAGTTCATGAAGCTG+AGG + chr2.4:34341327-34341346 MS.gene83236:CDS 45.0%
CCATCAGAAACACCAGTGTA+AGG - chr2.4:34339862-34339881 None:intergenic 45.0%
CGAATAGAATCCGGAGATTC+TGG + chr2.4:34341077-34341096 MS.gene83236:CDS 45.0%
CGTAATCTAGCTATGGCAAC+AGG + chr2.4:34341004-34341023 MS.gene83236:CDS 45.0%
CTCGGCTATTAAGCAGTTTC+TGG - chr2.4:34340922-34340941 None:intergenic 45.0%
CTTAGTCCTAGAGGCAATAC+AGG - chr2.4:34341241-34341260 None:intergenic 45.0%
GAATCAAATGCTAGGGACTC+AGG - chr2.4:34339826-34339845 None:intergenic 45.0%
GCAACATGGCTGTTATCACT+GGG + chr2.4:34339710-34339729 MS.gene83236:CDS 45.0%
GCATTTGATTCCATTGGTGG+AGG + chr2.4:34339835-34339854 MS.gene83236:CDS 45.0%
GTTGCTCCGTAATCTAGCTA+TGG + chr2.4:34340997-34341016 MS.gene83236:CDS 45.0%
TCGCCTTGTACTCACTTACA+AGG - chr2.4:34341060-34341079 None:intergenic 45.0%
TTGATGTTATCTGGTCTCCC+TGG - chr2.4:34341148-34341167 None:intergenic 45.0%
TTGCTCACATTTCACCAGAC+CGG + chr2.4:34339926-34339945 MS.gene83236:CDS 45.0%
! CCTTACACTGGTGTTTCTGA+TGG + chr2.4:34339859-34339878 MS.gene83236:CDS 45.0%
AAACATGTGGGAGGCCTATG+GGG + chr2.4:34340234-34340253 MS.gene83236:CDS 50.0%
AGAAACATGTGGGAGGCCTA+TGG + chr2.4:34340232-34340251 MS.gene83236:CDS 50.0%
AGTCCTAGAGGCAATACAGG+AGG - chr2.4:34341238-34341257 None:intergenic 50.0%
ATGTACTAGGCAGCCCTTTC+GGG + chr2.4:34340360-34340379 MS.gene83236:CDS 50.0%
CTTGTTGAGGAGTTTGGCAC+TGG + chr2.4:34341298-34341317 MS.gene83236:CDS 50.0%
GAAACATGTGGGAGGCCTAT+GGG + chr2.4:34340233-34340252 MS.gene83236:CDS 50.0%
GTACCTCCTGTATTGCCTCT+AGG + chr2.4:34341232-34341251 MS.gene83236:CDS 50.0%
GTCCTGTAAGCCAGAATCTC+CGG - chr2.4:34341090-34341109 None:intergenic 50.0%
GTGCCAAACTCCTCAACAAG+AGG - chr2.4:34341298-34341317 None:intergenic 50.0%
GTGCCTCTTGTTGAGGAGTT+TGG + chr2.4:34341292-34341311 MS.gene83236:CDS 50.0%
GTGGATGTCGATCCTAACAC+TGG + chr2.4:34340395-34340414 MS.gene83236:CDS 50.0%
TAGGCCTCCCACATGTTTCT+TGG - chr2.4:34340232-34340251 None:intergenic 50.0%
! AGAGGCAATACAGGAGGTAC+TGG - chr2.4:34341232-34341251 None:intergenic 50.0%
! AGATTAGACACGTACCGGTC+TGG - chr2.4:34339943-34339962 None:intergenic 50.0%
! GACCTCGAGGAACGAGTTTT+GGG + chr2.4:34341171-34341190 MS.gene83236:CDS 50.0%
!! AATACAGGAGGTACTGGTGG+TGG - chr2.4:34341226-34341245 None:intergenic 50.0%
!! ATCCGGAGATTCTGGCTTAC+AGG + chr2.4:34341085-34341104 MS.gene83236:CDS 50.0%
!! CAGTGTCGACGTGCTTCATA+GGG + chr2.4:34340074-34340093 MS.gene83236:intron 50.0%
ACGCCAGCAAGAAACCCGAA+AGG - chr2.4:34340377-34340396 None:intergenic 55.0%
AGAGGCTGTGCCTCTTGTTG+AGG + chr2.4:34341285-34341304 MS.gene83236:CDS 55.0%
AGTGTAAGGCCCTCCACCAA+TGG - chr2.4:34339848-34339867 None:intergenic 55.0%
ATTGGTGGAGGGCCTTACAC+TGG + chr2.4:34339847-34339866 MS.gene83236:CDS 55.0%
CACCCAAAACTCGTTCCTCG+AGG - chr2.4:34341176-34341195 None:intergenic 55.0%
CGCCAGCAAGAAACCCGAAA+GGG - chr2.4:34340376-34340395 None:intergenic 55.0%
GACTCAGGGCCTGTTAGTGA+TGG - chr2.4:34339811-34339830 None:intergenic 55.0%
GGGGTAGCAGTAAGCAGCAA+TGG + chr2.4:34341025-34341044 MS.gene83236:CDS 55.0%
TCAGGGCCTGTTAGTGATGG+TGG - chr2.4:34339808-34339827 None:intergenic 55.0%
! GGACCTCGAGGAACGAGTTT+TGG + chr2.4:34341170-34341189 MS.gene83236:CDS 55.0%
!! AGCCCTTTCGGGTTTCTTGC+TGG + chr2.4:34340371-34340390 MS.gene83236:CDS 55.0%
!! GCAGTGTCGACGTGCTTCAT+AGG + chr2.4:34340073-34340092 MS.gene83236:intron 55.0%
!! GGCAATACAGGAGGTACTGG+TGG - chr2.4:34341229-34341248 None:intergenic 55.0%
!! TTTCGGGTTTCTTGCTGGCG+TGG + chr2.4:34340376-34340395 MS.gene83236:CDS 55.0%
!!! CTCGAGGAACGAGTTTTGGG+TGG + chr2.4:34341174-34341193 MS.gene83236:CDS 55.0%
!! ACAGGAGGTACTGGTGGTGG+TGG - chr2.4:34341223-34341242 None:intergenic 60.0%
!! GGTGGTGGTGGTGACCCAAA+AGG - chr2.4:34341211-34341230 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 34339703 34341405 34339703 ID=MS.gene83236
chr2.4 mRNA 34339703 34341405 34339703 ID=MS.gene83236.t1;Parent=MS.gene83236
chr2.4 exon 34339703 34339947 34339703 ID=MS.gene83236.t1.exon1;Parent=MS.gene83236.t1
chr2.4 CDS 34339703 34339947 34339703 ID=cds.MS.gene83236.t1;Parent=MS.gene83236.t1
chr2.4 exon 34340187 34340459 34340187 ID=MS.gene83236.t1.exon2;Parent=MS.gene83236.t1
chr2.4 CDS 34340187 34340459 34340187 ID=cds.MS.gene83236.t1;Parent=MS.gene83236.t1
chr2.4 exon 34340913 34341405 34340913 ID=MS.gene83236.t1.exon3;Parent=MS.gene83236.t1
chr2.4 CDS 34340913 34341405 34340913 ID=cds.MS.gene83236.t1;Parent=MS.gene83236.t1
Gene Sequence

>MS.gene83236

ATGTCTAGCAACATGGCTGTTATCACTGGGATCTTAATCTCCCTGATACTCTATTCATCATCTATAGTTGCCTATTGCAAATTACTAGACAGACTCTATCTTCCACCATCACTAACAGGCCCTGAGTCCCTAGCATTTGATTCCATTGGTGGAGGGCCTTACACTGGTGTTTCTGATGGAAGAATTCTAAAATATGATAAAGAACGTTCTAGTTTCCTTGAGTTTGCTCACATTTCACCAGACCGGTACGTGTCTAATCTAAAATATGGTTTGAATCTCCGTCAAGAAAGATGTTCACATTTAGTATTGAACTTGACCATATAGGTTTAATCTGCTCTAAACTTTGTCAGCGTTTATGTTTTTGACATTAGCAGTGTCGACGTGCTTCATAGGGTTTCACTCCAAAATATAATACATAATAGTTCTTTTTTATGTAATTATAGTCATAGTTGGTTAAGGTTAAACCATGTCATTGTTGTTACAGGAACAAAACAATTTGTGATGGAATTTTAGACTTCTCAGAACTCCAAGAAACATGTGGGAGGCCTATGGGGTTGAGTTACAATTACAAGACGAAGGAATTATATATAGCTGATGCTTATTATGGACTTGTGAAGGTTTCTTATGATGGAGGTGCTGCAACACAACTTGTTTCTAATGTACTAGGCAGCCCTTTCGGGTTTCTTGCTGGCGTGGATGTCGATCCTAACACTGGAATCGTTTATTTCACGGAAGCTAGCTATTATCACAAGATCAGGTAATCCATTCTATTCTATCTCATGATTTTTCCATGTTTTTTCGCATTGCTATGCTTGATGATGATGTTAGCAGTGTGAAATGAACAATGTGAATGAGTTTGTGAACTCTGTACATTTGTGTATCTCAATTTGCCATGCCTGAATCATCAAGTCATCATGATCTTGAGATTCAGCACAAGTAACTTTTTAGACCTATAATATTCTCTTCTCAAAGCAAGTATATTAAAGGAAATAATAGCTTATTTAAGCGTCTAATAATTCATAAATCAATTTTTGCCTATACACTTTATAAACGCAACGATAATACTATGTTTCAACAATAAAATTCTTATGTTATTTTAATGATATGTTGCATTTTGAATTGATAGTAGTGAGTTCACACAAGAAATGTTCAATGTAGCAAATATTCTCTCTAGTCTTGGCCGATTAAAATTGTGACCTAAATTTTGCAGGGATCTCCAGAAACTGCTTAATAGCCGAGATATTTTTTCTGGAAGCCTGTTCAGATACAATCCAATAACGAAACAAACCACATTGTTGCTCCGTAATCTAGCTATGGCAACAGGGGTAGCAGTAAGCAGCAATGGTTCATTTGTCCTTGTAAGTGAGTACAAGGCGAATAGAATCCGGAGATTCTGGCTTACAGGACCAAATGCATATACTTCAGATATATTCTTGTGGCTTCCAGGGAGACCAGATAACATCAAAAGGACCTCGAGGAACGAGTTTTGGGTGGCAGTGAATTATCCTTTTGGGTCACCACCACCACCAGTACCTCCTGTATTGCCTCTAGGACTAAGAATCAATGAGCAAGGTTTAATTTTAGAGGCTGTGCCTCTTGTTGAGGAGTTTGGCACTGGATCAGTCAGTGAAGTTCATGAAGCTGAGGGAAAACTCTATGCTACATCGTTGCGTGATAGCTATGTTAACATTCTCACAGTCTAG
Protein sequence

>MS.gene83236.t1

MSSNMAVITGILISLILYSSSIVAYCKLLDRLYLPPSLTGPESLAFDSIGGGPYTGVSDGRILKYDKERSSFLEFAHISPDRNKTICDGILDFSELQETCGRPMGLSYNYKTKELYIADAYYGLVKVSYDGGAATQLVSNVLGSPFGFLAGVDVDPNTGIVYFTEASYYHKIRDLQKLLNSRDIFSGSLFRYNPITKQTTLLLRNLAMATGVAVSSNGSFVLVSEYKANRIRRFWLTGPNAYTSDIFLWLPGRPDNIKRTSRNEFWVAVNYPFGSPPPPVPPVLPLGLRINEQGLILEAVPLVEEFGTGSVSEVHEAEGKLYATSLRDSYVNILTV