Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82350.t1 | XP_003607781.1 | 91.7 | 121 | 7 | 1 | 1 | 118 | 1 | 121 | 1.00E-48 | 202.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82350.t1 | Q9ZQ80 | 55.9 | 68 | 27 | 1 | 15 | 79 | 68 | 135 | 4.2e-11 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82350.t1 | G7JGC9 | 91.7 | 121 | 7 | 1 | 1 | 118 | 1 | 121 | 7.3e-49 | 202.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene82350 | 0.828507 | 8.65E-55 | -1.69E-46 |
| MS.gene04917 | MS.gene82350 | 0.824823 | 6.55E-54 | -1.69E-46 |
| MS.gene049378 | MS.gene82350 | 0.834384 | 3.09E-56 | -1.69E-46 |
| MS.gene049438 | MS.gene82350 | 0.82757 | 1.45E-54 | -1.69E-46 |
| MS.gene04984 | MS.gene82350 | 0.800913 | 1.15E-48 | -1.69E-46 |
| MS.gene04991 | MS.gene82350 | -0.819112 | 1.38E-52 | -1.69E-46 |
| MS.gene050317 | MS.gene82350 | 0.816893 | 4.37E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene82350 | -0.832023 | 1.20E-55 | -1.69E-46 |
| MS.gene050629 | MS.gene82350 | -0.808952 | 2.41E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene82350 | 0.838232 | 3.25E-57 | -1.69E-46 |
| MS.gene050763 | MS.gene82350 | 0.83964 | 1.40E-57 | -1.69E-46 |
| MS.gene05119 | MS.gene82350 | -0.803421 | 3.51E-49 | -1.69E-46 |
| MS.gene051245 | MS.gene82350 | 0.811134 | 8.15E-51 | -1.69E-46 |
| MS.gene051535 | MS.gene82350 | 0.812683 | 3.75E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene82350 | 0.821602 | 3.70E-53 | -1.69E-46 |
| MS.gene052302 | MS.gene82350 | 0.809082 | 2.26E-50 | -1.69E-46 |
| MS.gene052309 | MS.gene82350 | -0.846924 | 1.60E-59 | -1.69E-46 |
| MS.gene052376 | MS.gene82350 | 0.811549 | 6.62E-51 | -1.69E-46 |
| MS.gene053216 | MS.gene82350 | 0.808516 | 2.98E-50 | -1.69E-46 |
| MS.gene054379 | MS.gene82350 | 0.839254 | 1.77E-57 | -1.69E-46 |
| MS.gene054536 | MS.gene82350 | 0.804566 | 2.03E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene82350 | 0.84821 | 7.09E-60 | -1.69E-46 |
| MS.gene055174 | MS.gene82350 | 0.808245 | 3.41E-50 | -1.69E-46 |
| MS.gene055441 | MS.gene82350 | 0.827005 | 1.99E-54 | -1.69E-46 |
| MS.gene055701 | MS.gene82350 | 0.81793 | 2.55E-52 | -1.69E-46 |
| MS.gene056464 | MS.gene82350 | -0.808398 | 3.16E-50 | -1.69E-46 |
| MS.gene056752 | MS.gene82350 | 0.806653 | 7.41E-50 | -1.69E-46 |
| MS.gene056849 | MS.gene82350 | 0.844544 | 7.08E-59 | -1.69E-46 |
| MS.gene057451 | MS.gene82350 | -0.83041 | 2.98E-55 | -1.69E-46 |
| MS.gene057452 | MS.gene82350 | -0.838117 | 3.48E-57 | -1.69E-46 |
| MS.gene05761 | MS.gene82350 | 0.837235 | 5.86E-57 | -1.69E-46 |
| MS.gene058182 | MS.gene82350 | 0.837706 | 4.44E-57 | -1.69E-46 |
| MS.gene058238 | MS.gene82350 | 0.800788 | 1.22E-48 | -1.69E-46 |
| MS.gene058661 | MS.gene82350 | -0.808972 | 2.38E-50 | -1.69E-46 |
| MS.gene059872 | MS.gene82350 | -0.836909 | 7.10E-57 | -1.69E-46 |
| MS.gene060084 | MS.gene82350 | 0.878422 | 2.84E-69 | -1.69E-46 |
| MS.gene060297 | MS.gene82350 | 0.859717 | 3.41E-63 | -1.69E-46 |
| MS.gene060869 | MS.gene82350 | 0.857261 | 1.84E-62 | -1.69E-46 |
| MS.gene060870 | MS.gene82350 | 0.838501 | 2.77E-57 | -1.69E-46 |
| MS.gene060940 | MS.gene82350 | 0.838518 | 2.74E-57 | -1.69E-46 |
| MS.gene060943 | MS.gene82350 | 0.800291 | 1.54E-48 | -1.69E-46 |
| MS.gene061002 | MS.gene82350 | 0.811506 | 6.77E-51 | -1.69E-46 |
| MS.gene061004 | MS.gene82350 | 0.824692 | 7.03E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82350.t1 | MTR_4g082860 | 90.909 | 121 | 8 | 1 | 1 | 118 | 1 | 121 | 1.56e-64 | 192 |
| MS.gene82350.t1 | MTR_4g082830 | 66.364 | 110 | 26 | 3 | 3 | 102 | 11 | 119 | 1.46e-37 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82350.t1 | AT2G03440 | 54.167 | 72 | 30 | 1 | 11 | 79 | 64 | 135 | 2.20e-13 | 63.5 |
| MS.gene82350.t1 | AT1G13930 | 47.581 | 124 | 45 | 8 | 8 | 117 | 33 | 150 | 5.36e-11 | 56.6 |
| MS.gene82350.t1 | AT1G13930 | 47.581 | 124 | 45 | 8 | 8 | 117 | 33 | 150 | 5.36e-11 | 56.6 |
| MS.gene82350.t1 | AT1G13930 | 47.581 | 124 | 45 | 8 | 8 | 117 | 33 | 150 | 5.36e-11 | 56.6 |
Find 25 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCAACCAGTGAGCTTA+TGG | 0.299153 | 4.2:-26872543 | MS.gene82350:CDS |
| AGGCAGCACAGTCAGGTTTC+GGG | 0.304942 | 4.2:-26872499 | MS.gene82350:CDS |
| GAGGCAGCACAGTCAGGTTT+CGG | 0.309235 | 4.2:-26872500 | MS.gene82350:CDS |
| CCACCTGATCCTCCACCTTC+AGG | 0.341637 | 4.2:+26872269 | None:intergenic |
| AGTTGGTCAGTATGCTAAAT+TGG | 0.398975 | 4.2:-26872423 | MS.gene82350:CDS |
| AAGCCTGAAGGTGGAGGATC+AGG | 0.423090 | 4.2:-26872272 | MS.gene82350:CDS |
| TGCACTTGCCATAAGCTCAC+TGG | 0.466727 | 4.2:+26872535 | None:intergenic |
| AGCTGCTGATTATCTGCATA+AGG | 0.469713 | 4.2:-26872366 | MS.gene82350:CDS |
| GGTGGAGGATCAGGTGGACT+AGG | 0.476662 | 4.2:-26872263 | MS.gene82350:CDS |
| GACAAGACAGCAGAAGCTGC+AGG | 0.506059 | 4.2:-26872461 | MS.gene82350:CDS |
| GTAGCAGAGGCAGCACAGTC+AGG | 0.527765 | 4.2:-26872506 | MS.gene82350:CDS |
| TGATCTGCAGGTTTAGAAGC+TGG | 0.532370 | 4.2:+26872317 | None:intergenic |
| AAGGTTGAGAACACTACTAC+TGG | 0.547092 | 4.2:-26872347 | MS.gene82350:CDS |
| AAGTGCAAAGGTGGTAGCAG+AGG | 0.549500 | 4.2:-26872519 | MS.gene82350:CDS |
| TTGGATGAACAGAAAGGTGT+AGG | 0.558648 | 4.2:-26872404 | MS.gene82350:CDS |
| GCTAAATTGGATGAACAGAA+AGG | 0.567227 | 4.2:-26872410 | MS.gene82350:CDS |
| CCTGAAGGTGGAGGATCAGG+TGG | 0.601078 | 4.2:-26872269 | MS.gene82350:CDS |
| TGAGCTTATGGCAAGTGCAA+AGG | 0.606848 | 4.2:-26872531 | MS.gene82350:CDS |
| GGAGGATCAGGTGGACTAGG+TGG | 0.611533 | 4.2:-26872260 | MS.gene82350:CDS |
| GCTTATGGCAAGTGCAAAGG+TGG | 0.622253 | 4.2:-26872528 | MS.gene82350:CDS |
| GGTGATCTTCTAGATGCAGT+TGG | 0.625884 | 4.2:-26872440 | MS.gene82350:CDS |
| GTTGCTAAGCCTGAAGGTGG+AGG | 0.637775 | 4.2:-26872278 | MS.gene82350:CDS |
| TCTGCAGGTTTAGAAGCTGG+TGG | 0.662750 | 4.2:+26872320 | None:intergenic |
| GATGATGTTGCTAAGCCTGA+AGG | 0.667542 | 4.2:-26872284 | MS.gene82350:CDS |
| GATGTTGCTAAGCCTGAAGG+TGG | 0.674394 | 4.2:-26872281 | MS.gene82350:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGCAACATCATCACTTTT+AGG | + | chr4.2:26872516-26872535 | None:intergenic | 30.0% |
| AGTTGGTCAGTATGCTAAAT+TGG | - | chr4.2:26872383-26872402 | MS.gene82350:CDS | 35.0% | |
| GCTAAATTGGATGAACAGAA+AGG | - | chr4.2:26872396-26872415 | MS.gene82350:CDS | 35.0% | |
| AAGGTTGAGAACACTACTAC+TGG | - | chr4.2:26872459-26872478 | MS.gene82350:CDS | 40.0% | |
| GATTTCAACCAGTGAGCTTA+TGG | - | chr4.2:26872263-26872282 | MS.gene82350:CDS | 40.0% | |
| ! | ACTAGGTGGTGATTTTGCTA+AGG | - | chr4.2:26872560-26872579 | MS.gene82350:CDS | 40.0% |
| ! | AGCTGCTGATTATCTGCATA+AGG | - | chr4.2:26872440-26872459 | MS.gene82350:CDS | 40.0% |
| ! | TTGGATGAACAGAAAGGTGT+AGG | - | chr4.2:26872402-26872421 | MS.gene82350:CDS | 40.0% |
| GATGATGTTGCTAAGCCTGA+AGG | - | chr4.2:26872522-26872541 | MS.gene82350:CDS | 45.0% | |
| GGTGATCTTCTAGATGCAGT+TGG | - | chr4.2:26872366-26872385 | MS.gene82350:CDS | 45.0% | |
| TGAGCTTATGGCAAGTGCAA+AGG | - | chr4.2:26872275-26872294 | MS.gene82350:CDS | 45.0% | |
| TGATCTGCAGGTTTAGAAGC+TGG | + | chr4.2:26872492-26872511 | None:intergenic | 45.0% | |
| ! | GGTGATTTTGCTAAGGTTGC+TGG | - | chr4.2:26872567-26872586 | MS.gene82350:CDS | 45.0% |
| !! | TCACTTTTAGGCTGATCTGC+AGG | + | chr4.2:26872504-26872523 | None:intergenic | 45.0% |
| AAGTGCAAAGGTGGTAGCAG+AGG | - | chr4.2:26872287-26872306 | MS.gene82350:CDS | 50.0% | |
| GATGTTGCTAAGCCTGAAGG+TGG | - | chr4.2:26872525-26872544 | MS.gene82350:CDS | 50.0% | |
| GCTTATGGCAAGTGCAAAGG+TGG | - | chr4.2:26872278-26872297 | MS.gene82350:CDS | 50.0% | |
| TCTGCAGGTTTAGAAGCTGG+TGG | + | chr4.2:26872489-26872508 | None:intergenic | 50.0% | |
| TGCACTTGCCATAAGCTCAC+TGG | + | chr4.2:26872274-26872293 | None:intergenic | 50.0% | |
| AAGCCTGAAGGTGGAGGATC+AGG | - | chr4.2:26872534-26872553 | MS.gene82350:CDS | 55.0% | |
| AGGCAGCACAGTCAGGTTTC+GGG | - | chr4.2:26872307-26872326 | MS.gene82350:CDS | 55.0% | |
| GACAAGACAGCAGAAGCTGC+AGG | - | chr4.2:26872345-26872364 | MS.gene82350:CDS | 55.0% | |
| GAGGCAGCACAGTCAGGTTT+CGG | - | chr4.2:26872306-26872325 | MS.gene82350:CDS | 55.0% | |
| GTTGCTAAGCCTGAAGGTGG+AGG | - | chr4.2:26872528-26872547 | MS.gene82350:CDS | 55.0% | |
| CCACCTGATCCTCCACCTTC+AGG | + | chr4.2:26872540-26872559 | None:intergenic | 60.0% | |
| CCTGAAGGTGGAGGATCAGG+TGG | - | chr4.2:26872537-26872556 | MS.gene82350:CDS | 60.0% | |
| GTAGCAGAGGCAGCACAGTC+AGG | - | chr4.2:26872300-26872319 | MS.gene82350:CDS | 60.0% | |
| ! | GGTGGAGGATCAGGTGGACT+AGG | - | chr4.2:26872543-26872562 | MS.gene82350:CDS | 60.0% |
| !! | GGAGGATCAGGTGGACTAGG+TGG | - | chr4.2:26872546-26872565 | MS.gene82350:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26872237 | 26872591 | 26872237 | ID=MS.gene82350 |
| chr4.2 | mRNA | 26872237 | 26872591 | 26872237 | ID=MS.gene82350.t1;Parent=MS.gene82350 |
| chr4.2 | exon | 26872237 | 26872591 | 26872237 | ID=MS.gene82350.t1.exon1;Parent=MS.gene82350.t1 |
| chr4.2 | CDS | 26872237 | 26872591 | 26872237 | ID=cds.MS.gene82350.t1;Parent=MS.gene82350.t1 |
| Gene Sequence |
| Protein sequence |
