AlfalfaGEDB | MS.gene22314

M. sativa cultivar XinJiangDaYe / MS.gene22314

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22314.t1	XP_003611004.1	99.5	182	0	1	1	181	139	320	9.70E-67	263.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22314.t1	Q9C7W2	58.5	188	59	4	1	180	158	334	2.4e-29	130.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22314.t1	G7KAL6	99.5	182	0	1	1	181	139	320	7.0e-67	263.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene22314.t1	TF	AP2/ERF-ERF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene22314	0.816399	5.64E-52	-1.69E-46
MS.gene049378	MS.gene22314	0.813103	3.03E-51	-1.69E-46
MS.gene049424	MS.gene22314	0.818614	1.79E-52	-1.69E-46
MS.gene049438	MS.gene22314	0.805878	1.08E-49	-1.69E-46
MS.gene049653	MS.gene22314	0.810496	1.12E-50	-1.69E-46
MS.gene049703	MS.gene22314	-0.800532	1.38E-48	-1.69E-46
MS.gene049756	MS.gene22314	0.813177	2.92E-51	-1.69E-46
MS.gene04988	MS.gene22314	0.807802	4.23E-50	-1.69E-46
MS.gene050226	MS.gene22314	0.809954	1.47E-50	-1.69E-46
MS.gene050227	MS.gene22314	0.810107	1.36E-50	-1.69E-46
MS.gene050228	MS.gene22314	0.816731	4.75E-52	-1.69E-46
MS.gene050277	MS.gene22314	0.850567	1.56E-60	-1.69E-46
MS.gene050303	MS.gene22314	0.808183	3.51E-50	-1.69E-46
MS.gene050623	MS.gene22314	-0.833807	4.31E-56	-1.69E-46
MS.gene050987	MS.gene22314	0.841973	3.43E-58	-1.69E-46
MS.gene051063	MS.gene22314	0.805298	1.43E-49	-1.69E-46
MS.gene051243	MS.gene22314	0.815342	9.71E-52	-1.69E-46
MS.gene051245	MS.gene22314	0.837033	6.60E-57	-1.69E-46
MS.gene051349	MS.gene22314	0.825954	3.54E-54	-1.69E-46
MS.gene051451	MS.gene22314	0.800349	1.50E-48	-1.69E-46
MS.gene051633	MS.gene22314	0.834691	2.59E-56	-1.69E-46
MS.gene051662	MS.gene22314	0.825589	4.32E-54	-1.69E-46
MS.gene051748	MS.gene22314	0.802844	4.63E-49	-1.69E-46
MS.gene051757	MS.gene22314	0.803831	2.89E-49	-1.69E-46
MS.gene051903	MS.gene22314	0.817098	3.93E-52	-1.69E-46
MS.gene052176	MS.gene22314	0.846698	1.84E-59	-1.69E-46
MS.gene052286	MS.gene22314	0.857159	1.97E-62	-1.69E-46
MS.gene052302	MS.gene22314	0.879711	9.97E-70	-1.69E-46
MS.gene052309	MS.gene22314	-0.851429	8.93E-61	-1.69E-46
MS.gene052376	MS.gene22314	0.870048	1.96E-66	-1.69E-46
MS.gene052600	MS.gene22314	0.802315	5.95E-49	-1.69E-46
MS.gene052856	MS.gene22314	0.827831	1.26E-54	-1.69E-46
MS.gene052858	MS.gene22314	0.826768	2.26E-54	-1.69E-46
MS.gene052860	MS.gene22314	0.828445	8.96E-55	-1.69E-46
MS.gene053188	MS.gene22314	0.82258	2.20E-53	-1.69E-46
MS.gene053189	MS.gene22314	0.815998	6.94E-52	-1.69E-46
MS.gene053216	MS.gene22314	0.835626	1.50E-56	-1.69E-46
MS.gene053630	MS.gene22314	0.812406	4.31E-51	-1.69E-46
MS.gene054536	MS.gene22314	0.812576	3.95E-51	-1.69E-46
MS.gene054537	MS.gene22314	0.823697	1.20E-53	-1.69E-46
MS.gene054699	MS.gene22314	0.82344	1.38E-53	-1.69E-46
MS.gene054930	MS.gene22314	0.833646	4.73E-56	-1.69E-46
MS.gene054942	MS.gene22314	0.816653	4.95E-52	-1.69E-46
MS.gene055174	MS.gene22314	0.803565	3.28E-49	-1.69E-46
MS.gene055441	MS.gene22314	0.829723	4.39E-55	-1.69E-46
MS.gene055591	MS.gene22314	-0.807878	4.08E-50	-1.69E-46
MS.gene056069	MS.gene22314	-0.823148	1.62E-53	-1.69E-46
MS.gene056631	MS.gene22314	0.841526	4.50E-58	-1.69E-46
MS.gene056760	MS.gene22314	0.824784	6.69E-54	-1.69E-46
MS.gene057057	MS.gene22314	0.807985	3.87E-50	-1.69E-46
MS.gene057079	MS.gene22314	0.857253	1.85E-62	-1.69E-46
MS.gene057140	MS.gene22314	0.805524	1.28E-49	-1.69E-46
MS.gene057234	MS.gene22314	0.833466	5.25E-56	-1.69E-46
MS.gene057260	MS.gene22314	0.802993	4.31E-49	-1.69E-46
MS.gene057392	MS.gene22314	0.800135	1.66E-48	-1.69E-46
MS.gene057451	MS.gene22314	-0.826106	3.25E-54	-1.69E-46
MS.gene057452	MS.gene22314	-0.821764	3.39E-53	-1.69E-46
MS.gene057555	MS.gene22314	0.82263	2.14E-53	-1.69E-46
MS.gene057754	MS.gene22314	0.808955	2.40E-50	-1.69E-46
MS.gene05795	MS.gene22314	0.840199	1.00E-57	-1.69E-46
MS.gene058055	MS.gene22314	0.819664	1.03E-52	-1.69E-46
MS.gene058182	MS.gene22314	0.850685	1.45E-60	-1.69E-46
MS.gene058201	MS.gene22314	0.802736	4.87E-49	-1.69E-46
MS.gene058238	MS.gene22314	0.85119	1.04E-60	-1.69E-46
MS.gene058661	MS.gene22314	-0.839884	1.21E-57	-1.69E-46
MS.gene058749	MS.gene22314	0.804668	1.93E-49	-1.69E-46
MS.gene058906	MS.gene22314	0.838703	2.46E-57	-1.69E-46
MS.gene059569	MS.gene22314	0.802173	6.36E-49	-1.69E-46
MS.gene059872	MS.gene22314	-0.829351	5.41E-55	-1.69E-46
MS.gene059899	MS.gene22314	0.806017	1.01E-49	-1.69E-46
MS.gene06001	MS.gene22314	0.83758	4.78E-57	-1.69E-46
MS.gene060084	MS.gene22314	0.825857	3.73E-54	-1.69E-46
MS.gene060297	MS.gene22314	0.861368	1.08E-63	-1.69E-46
MS.gene060476	MS.gene22314	0.802613	5.16E-49	-1.69E-46
MS.gene060619	MS.gene22314	0.808493	3.01E-50	-1.69E-46
MS.gene061113	MS.gene22314	0.800868	1.18E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22314.t1	MTR_5g009410	98.901	182	1	1	1	181	139	320	2.47e-129	365
MS.gene22314.t1	MTR_3g074130	45.070	142	56	4	2	123	173	312	1.52e-25	100
MS.gene22314.t1	MTR_5g062700	57.971	69	25	2	2	69	196	261	1.37e-20	87.8
MS.gene22314.t1	MTR_1g090170	35.359	181	74	6	2	181	175	313	1.15e-18	81.6
MS.gene22314.t1	MTR_1g090170	35.359	181	74	6	2	181	262	400	1.19e-18	82.4
MS.gene22314.t1	MTR_8g090350	55.882	68	22	2	2	69	136	195	1.53e-18	80.9
MS.gene22314.t1	MTR_8g006840	65.306	49	17	0	2	50	149	197	3.89e-13	65.1
MS.gene22314.t1	MTR_8g006815	73.684	38	10	0	2	39	120	157	8.28e-13	64.3
MS.gene22314.t1	MTR_8g006825	62.222	45	14	1	2	43	120	164	3.97e-12	62.4
MS.gene22314.t1	MTR_2g090235	75.000	36	9	0	2	37	241	276	4.78e-12	63.5
MS.gene22314.t1	MTR_7g081795	66.667	36	12	0	2	37	84	119	2.95e-11	60.1
MS.gene22314.t1	MTR_4g100380	76.471	34	8	0	2	35	169	202	6.00e-11	60.1
MS.gene22314.t1	MTR_2g015040	73.529	34	9	0	2	35	87	120	6.50e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22314.t1	AT1G64380	52.406	187	72	6	1	180	158	334	5.16e-33	120
MS.gene22314.t1	AT1G78080	44.828	145	64	5	2	131	175	318	1.40e-25	100
MS.gene22314.t1	AT4G39780	55.714	70	30	1	2	71	116	184	1.29e-20	86.3
MS.gene22314.t1	AT1G22190	50.000	76	30	1	2	77	106	173	2.16e-19	82.8
MS.gene22314.t1	AT2G22200	51.429	70	25	1	2	71	94	154	1.85e-17	77.8
MS.gene22314.t1	AT1G36060	50.000	74	37	0	2	75	166	239	3.42e-17	77.8
MS.gene22314.t1	AT4G13620	32.402	179	71	5	2	180	255	383	1.68e-14	70.9
MS.gene22314.t1	AT5G65130	49.231	65	26	2	2	66	134	191	4.44e-13	65.9
MS.gene22314.t1	AT5G65130	49.231	65	26	2	2	66	136	193	4.56e-13	65.9
MS.gene22314.t1	AT2G44840	75.000	36	9	0	2	37	116	151	5.37e-12	62.4
MS.gene22314.t1	AT2G20880	75.000	36	9	0	2	37	210	245	8.55e-12	62.8

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGTTGGATCTTTCAAATT+TGG	0.137255	5.4:-4078334	None:intergenic
TTGGGAAGTTCTTGCTGTTT+AGG	0.206134	5.4:+4078756	MS.gene22314:CDS
CTGTTTCTGAGGATGGAATT+TGG	0.226527	5.4:+4078629	MS.gene22314:CDS
TGGGAAGTTCTTGCTGTTTA+GGG	0.257729	5.4:+4078757	MS.gene22314:CDS
GATCCAACTAAGTTGGGTTT+TGG	0.259771	5.4:+4078346	MS.gene22314:CDS
AGAACTTCCCAAATTAATTC+AGG	0.295502	5.4:-4078745	None:intergenic
CACAGAATAGAATGAGAGTT+TGG	0.304146	5.4:+4078221	None:intergenic
AAGAAGATGAAGAAGAATTC+AGG	0.374931	5.4:+4078469	MS.gene22314:CDS
TTTGAAAGATCCAACTAAGT+TGG	0.380517	5.4:+4078339	MS.gene22314:CDS
TTGAAAGATCCAACTAAGTT+GGG	0.405515	5.4:+4078340	MS.gene22314:CDS
ACCATTGTCGAAACAGAATT+CGG	0.441031	5.4:-4078667	None:intergenic
CTGAGGATGGAATTTGGAAA+GGG	0.444826	5.4:+4078635	MS.gene22314:CDS
CTATTTGCCAAAAGGTGAAA+AGG	0.446134	5.4:+4078422	MS.gene22314:CDS
TCTCCAAAACCCAACTTAGT+TGG	0.464313	5.4:-4078349	None:intergenic
ATAAGCATAAGCTGCAGCTT+CGG	0.474985	5.4:-4078263	None:intergenic
TCACCAACTGTTTCTGAGGA+TGG	0.477469	5.4:+4078622	MS.gene22314:CDS
AATTCAAGCTATTTGCCAAA+AGG	0.481211	5.4:+4078414	MS.gene22314:CDS
TATTTGCCAAAAGGTGAAAA+GGG	0.519794	5.4:+4078423	MS.gene22314:CDS
CGTGCAGCTTATAAGCTTCG+AGG	0.521878	5.4:+4078289	MS.gene22314:CDS
AAAAGATGGCATTCTTGCTA+AGG	0.531887	5.4:-4078719	None:intergenic
ATTAATTCAGGATCAAAAGA+TGG	0.531968	5.4:-4078733	None:intergenic
TCTGAGGATGGAATTTGGAA+AGG	0.539276	5.4:+4078634	MS.gene22314:CDS
GCCGAATTCTGTTTCGACAA+TGG	0.539512	5.4:+4078666	MS.gene22314:CDS
TGAGGATGGAATTTGGAAAG+GGG	0.545281	5.4:+4078636	MS.gene22314:CDS
AATAGAATGAGAGTTTGGCT+TGG	0.577666	5.4:+4078226	None:intergenic
ATTCCATCCTCAGAAACAGT+TGG	0.628763	5.4:-4078625	None:intergenic
TGTTTCGACAATGGTGACAG+AGG	0.638298	5.4:+4078675	MS.gene22314:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTGATCCTGAATTAATTT+GGG	+	chr5.4:4078738-4078757	MS.gene22314:CDS	20.0%
!	ATTAATTCAGGATCAAAAGA+TGG	-	chr5.4:4078736-4078755	None:intergenic	25.0%
!	TTAGTTGGATCTTTCAAATT+TGG	-	chr5.4:4078337-4078356	None:intergenic	25.0%
!!	CTTTTGATCCTGAATTAATT+TGG	+	chr5.4:4078737-4078756	MS.gene22314:CDS	25.0%
!!!	TCTTGTTTTTTGTGATGATT+GGG	+	chr5.4:4078585-4078604	MS.gene22314:CDS	25.0%
	AAGAAGATGAAGAAGAATTC+AGG	+	chr5.4:4078469-4078488	MS.gene22314:CDS	30.0%
	AATTCAAGCTATTTGCCAAA+AGG	+	chr5.4:4078414-4078433	MS.gene22314:CDS	30.0%
	AGAACTTCCCAAATTAATTC+AGG	-	chr5.4:4078748-4078767	None:intergenic	30.0%
	CAATCATCACAAAAAACAAG+AGG	-	chr5.4:4078586-4078605	None:intergenic	30.0%
	TATTTGCCAAAAGGTGAAAA+GGG	+	chr5.4:4078423-4078442	MS.gene22314:CDS	30.0%
	TTGAAAGATCCAACTAAGTT+GGG	+	chr5.4:4078340-4078359	MS.gene22314:CDS	30.0%
	TTTGAAAGATCCAACTAAGT+TGG	+	chr5.4:4078339-4078358	MS.gene22314:CDS	30.0%
!!!	CTCTTGTTTTTTGTGATGAT+TGG	+	chr5.4:4078584-4078603	MS.gene22314:CDS	30.0%
	AAAAGATGGCATTCTTGCTA+AGG	-	chr5.4:4078722-4078741	None:intergenic	35.0%
	ACCATTGTCGAAACAGAATT+CGG	-	chr5.4:4078670-4078689	None:intergenic	35.0%
	CAAAAAACAAGAGGTGATAG+CGG	-	chr5.4:4078577-4078596	None:intergenic	35.0%
	CTATTTGCCAAAAGGTGAAA+AGG	+	chr5.4:4078422-4078441	MS.gene22314:CDS	35.0%
!	CTTTTTCCCTTTTCACCTTT+TGG	-	chr5.4:4078432-4078451	None:intergenic	35.0%
	ATAAGCATAAGCTGCAGCTT+CGG	-	chr5.4:4078266-4078285	None:intergenic	40.0%
	ATTCCATCCTCAGAAACAGT+TGG	-	chr5.4:4078628-4078647	None:intergenic	40.0%
	CTGAGGATGGAATTTGGAAA+GGG	+	chr5.4:4078635-4078654	MS.gene22314:CDS	40.0%
	TCTCCAAAACCCAACTTAGT+TGG	-	chr5.4:4078352-4078371	None:intergenic	40.0%
	TCTGAGGATGGAATTTGGAA+AGG	+	chr5.4:4078634-4078653	MS.gene22314:CDS	40.0%
	TGAGGATGGAATTTGGAAAG+GGG	+	chr5.4:4078636-4078655	MS.gene22314:CDS	40.0%
!!	CTGTTTCTGAGGATGGAATT+TGG	+	chr5.4:4078629-4078648	MS.gene22314:CDS	40.0%
!!	GATCCAACTAAGTTGGGTTT+TGG	+	chr5.4:4078346-4078365	MS.gene22314:CDS	40.0%
!!!	GTTTTCACCAACTGTTTCTG+AGG	+	chr5.4:4078618-4078637	MS.gene22314:CDS	40.0%
	GCCGAATTCTGTTTCGACAA+TGG	+	chr5.4:4078666-4078685	MS.gene22314:CDS	45.0%
	TGTTTCGACAATGGTGACAG+AGG	+	chr5.4:4078675-4078694	MS.gene22314:CDS	45.0%
!!	TCACCAACTGTTTCTGAGGA+TGG	+	chr5.4:4078622-4078641	MS.gene22314:CDS	45.0%
	CGTGCAGCTTATAAGCTTCG+AGG	+	chr5.4:4078289-4078308	MS.gene22314:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	4078232	4078777	4078232	ID=MS.gene22314
chr5.4	mRNA	4078232	4078777	4078232	ID=MS.gene22314.t1;Parent=MS.gene22314
chr5.4	exon	4078232	4078777	4078232	ID=MS.gene22314.t1.exon1;Parent=MS.gene22314.t1
chr5.4	CDS	4078232	4078777	4078232	ID=cds.MS.gene22314.t1;Parent=MS.gene22314.t1

Gene Sequence

>MS.gene22314

ATGAGAGTTTGGCTTGGAACTTATGAAACTGCCGAAGCTGCAGCTTATGCTTATGATCGTGCAGCTTATAAGCTTCGAGGCGAATACGCGCGTTTAAATTTTCCAAATTTGAAAGATCCAACTAAGTTGGGTTTTGGAGATTCTACTAGATTGAATGCTTTGAAGAATTCTGTTGATGCTAAAATTCAAGCTATTTGCCAAAAGGTGAAAAGGGAAAAAGCTAAAAAGATTGCTGCAAAGAAGATGAAGAAGAATTCAGGTAGTAGTAATGATACAAAGAGTGATAAAAATTCAGACAAGATTATTAATTCATCTTCTTGTTCTTCTTCTTCGTCTTCGTTACCGCTATCACCTCTTGTTTTTTGTGATGATTGGGTGAATGAATTGTTTTCACCAACTGTTTCTGAGGATGGAATTTGGAAAGGGGAGAATTCGCCGAATTCTGTTTCGACAATGGTGACAGAGGAAACAGAGTTTGAAGATTGTTCCTTAGCAAGAATGCCATCTTTTGATCCTGAATTAATTTGGGAAGTTCTTGCTGTTTAG

Protein sequence

>MS.gene22314.t1

MRVWLGTYETAEAAAYAYDRAAYKLRGEYARLNFPNLKDPTKLGFGDSTRLNALKNSVDAKIQAICQKVKREKAKKIAAKKMKKNSGSSNDTKSDKNSDKIINSSSCSSSSSSLPLSPLVFCDDWVNELFSPTVSEDGIWKGENSPNSVSTMVTEETEFEDCSLARMPSFDPELIWEVLAV