Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82976.t1 | XP_003607781.1 | 91.7 | 121 | 7 | 1 | 1 | 118 | 1 | 121 | 1.00E-48 | 202.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82976.t1 | Q9ZQ80 | 55.9 | 68 | 27 | 1 | 15 | 79 | 68 | 135 | 4.2e-11 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82976.t1 | G7JGC9 | 91.7 | 121 | 7 | 1 | 1 | 118 | 1 | 121 | 7.3e-49 | 202.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene82976 | 0.814823 | 1.27E-51 | -1.69E-46 |
| MS.gene04917 | MS.gene82976 | 0.810654 | 1.04E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene82976 | 0.826644 | 2.42E-54 | -1.69E-46 |
| MS.gene049438 | MS.gene82976 | 0.820929 | 5.29E-53 | -1.69E-46 |
| MS.gene04991 | MS.gene82976 | -0.814194 | 1.74E-51 | -1.69E-46 |
| MS.gene050317 | MS.gene82976 | 0.809505 | 1.83E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene82976 | -0.827818 | 1.27E-54 | -1.69E-46 |
| MS.gene050762 | MS.gene82976 | 0.821408 | 4.10E-53 | -1.69E-46 |
| MS.gene050763 | MS.gene82976 | 0.822522 | 2.26E-53 | -1.69E-46 |
| MS.gene051245 | MS.gene82976 | 0.800489 | 1.41E-48 | -1.69E-46 |
| MS.gene051535 | MS.gene82976 | 0.804575 | 2.02E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene82976 | 0.820774 | 5.74E-53 | -1.69E-46 |
| MS.gene052302 | MS.gene82976 | 0.810685 | 1.02E-50 | -1.69E-46 |
| MS.gene052309 | MS.gene82976 | -0.84871 | 5.15E-60 | -1.69E-46 |
| MS.gene052376 | MS.gene82976 | 0.813077 | 3.07E-51 | -1.69E-46 |
| MS.gene053216 | MS.gene82976 | 0.804804 | 1.81E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene82976 | 0.82706 | 1.93E-54 | -1.69E-46 |
| MS.gene054536 | MS.gene82976 | 0.804133 | 2.50E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene82976 | 0.834335 | 3.18E-56 | -1.69E-46 |
| MS.gene055063 | MS.gene82976 | -0.805966 | 1.03E-49 | -1.69E-46 |
| MS.gene055441 | MS.gene82976 | 0.821133 | 4.75E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene82976 | 0.822884 | 1.87E-53 | -1.69E-46 |
| MS.gene056087 | MS.gene82976 | 0.801411 | 9.12E-49 | -1.69E-46 |
| MS.gene056752 | MS.gene82976 | 0.802113 | 6.54E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene82976 | 0.839403 | 1.62E-57 | -1.69E-46 |
| MS.gene056975 | MS.gene82976 | 0.802908 | 4.49E-49 | -1.69E-46 |
| MS.gene057451 | MS.gene82976 | -0.820859 | 5.49E-53 | -1.69E-46 |
| MS.gene057452 | MS.gene82976 | -0.835502 | 1.62E-56 | -1.69E-46 |
| MS.gene05761 | MS.gene82976 | 0.826282 | 2.96E-54 | -1.69E-46 |
| MS.gene058182 | MS.gene82976 | 0.823818 | 1.13E-53 | -1.69E-46 |
| MS.gene058238 | MS.gene82976 | 0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene058661 | MS.gene82976 | -0.801963 | 7.03E-49 | -1.69E-46 |
| MS.gene059872 | MS.gene82976 | -0.841824 | 3.76E-58 | -1.69E-46 |
| MS.gene060084 | MS.gene82976 | 0.865155 | 7.25E-65 | -1.69E-46 |
| MS.gene060297 | MS.gene82976 | 0.861475 | 1.00E-63 | -1.69E-46 |
| MS.gene060869 | MS.gene82976 | 0.845942 | 2.96E-59 | -1.69E-46 |
| MS.gene060870 | MS.gene82976 | 0.828718 | 7.69E-55 | -1.69E-46 |
| MS.gene060940 | MS.gene82976 | 0.827877 | 1.23E-54 | -1.69E-46 |
| MS.gene061002 | MS.gene82976 | 0.802497 | 5.45E-49 | -1.69E-46 |
| MS.gene061004 | MS.gene82976 | 0.814748 | 1.32E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82976.t1 | MTR_4g082860 | 90.909 | 121 | 8 | 1 | 1 | 118 | 1 | 121 | 1.56e-64 | 192 |
| MS.gene82976.t1 | MTR_4g082830 | 66.364 | 110 | 26 | 3 | 3 | 102 | 11 | 119 | 1.46e-37 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82976.t1 | AT2G03440 | 54.167 | 72 | 30 | 1 | 11 | 79 | 64 | 135 | 2.20e-13 | 63.5 |
| MS.gene82976.t1 | AT1G13930 | 47.581 | 124 | 45 | 8 | 8 | 117 | 33 | 150 | 5.36e-11 | 56.6 |
| MS.gene82976.t1 | AT1G13930 | 47.581 | 124 | 45 | 8 | 8 | 117 | 33 | 150 | 5.36e-11 | 56.6 |
| MS.gene82976.t1 | AT1G13930 | 47.581 | 124 | 45 | 8 | 8 | 117 | 33 | 150 | 5.36e-11 | 56.6 |
Find 25 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCAACCAGTGAGCTTA+TGG | 0.299153 | 4.2:-26860519 | MS.gene82976:CDS |
| AGGCAGCACAGTCAGGTTTC+GGG | 0.304942 | 4.2:-26860475 | MS.gene82976:CDS |
| GAGGCAGCACAGTCAGGTTT+CGG | 0.309235 | 4.2:-26860476 | MS.gene82976:CDS |
| CCACCTGATCCTCCACCTTC+AGG | 0.341637 | 4.2:+26860245 | None:intergenic |
| AGTTGGTCAGTATGCTAAAT+TGG | 0.398975 | 4.2:-26860399 | MS.gene82976:CDS |
| AAGCCTGAAGGTGGAGGATC+AGG | 0.423090 | 4.2:-26860248 | MS.gene82976:CDS |
| TGCACTTGCCATAAGCTCAC+TGG | 0.466727 | 4.2:+26860511 | None:intergenic |
| AGCTGCTGATTATCTGCATA+AGG | 0.469713 | 4.2:-26860342 | MS.gene82976:CDS |
| GGTGGAGGATCAGGTGGACT+AGG | 0.476662 | 4.2:-26860239 | MS.gene82976:CDS |
| GACAAGACAGCAGAAGCTGC+AGG | 0.506059 | 4.2:-26860437 | MS.gene82976:CDS |
| GTAGCAGAGGCAGCACAGTC+AGG | 0.527765 | 4.2:-26860482 | MS.gene82976:CDS |
| TGATCTGCAGGTTTAGAAGC+TGG | 0.532370 | 4.2:+26860293 | None:intergenic |
| AAGGTTGAGAACACTACTAC+TGG | 0.547092 | 4.2:-26860323 | MS.gene82976:CDS |
| AAGTGCAAAGGTGGTAGCAG+AGG | 0.549500 | 4.2:-26860495 | MS.gene82976:CDS |
| TTGGATGAACAGAAAGGTGT+AGG | 0.558648 | 4.2:-26860380 | MS.gene82976:CDS |
| GCTAAATTGGATGAACAGAA+AGG | 0.567227 | 4.2:-26860386 | MS.gene82976:CDS |
| CCTGAAGGTGGAGGATCAGG+TGG | 0.601078 | 4.2:-26860245 | MS.gene82976:CDS |
| TGAGCTTATGGCAAGTGCAA+AGG | 0.606848 | 4.2:-26860507 | MS.gene82976:CDS |
| GGAGGATCAGGTGGACTAGG+TGG | 0.611533 | 4.2:-26860236 | MS.gene82976:CDS |
| GCTTATGGCAAGTGCAAAGG+TGG | 0.622253 | 4.2:-26860504 | MS.gene82976:CDS |
| GGTGATCTTCTAGATGCAGT+TGG | 0.625884 | 4.2:-26860416 | MS.gene82976:CDS |
| GTTGCTAAGCCTGAAGGTGG+AGG | 0.637775 | 4.2:-26860254 | MS.gene82976:CDS |
| TCTGCAGGTTTAGAAGCTGG+TGG | 0.662750 | 4.2:+26860296 | None:intergenic |
| GATGATGTTGCTAAGCCTGA+AGG | 0.667542 | 4.2:-26860260 | MS.gene82976:CDS |
| GATGTTGCTAAGCCTGAAGG+TGG | 0.674394 | 4.2:-26860257 | MS.gene82976:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGCAACATCATCACTTTT+AGG | + | chr4.2:26860492-26860511 | None:intergenic | 30.0% |
| AGTTGGTCAGTATGCTAAAT+TGG | - | chr4.2:26860359-26860378 | MS.gene82976:CDS | 35.0% | |
| GCTAAATTGGATGAACAGAA+AGG | - | chr4.2:26860372-26860391 | MS.gene82976:CDS | 35.0% | |
| AAGGTTGAGAACACTACTAC+TGG | - | chr4.2:26860435-26860454 | MS.gene82976:CDS | 40.0% | |
| GATTTCAACCAGTGAGCTTA+TGG | - | chr4.2:26860239-26860258 | MS.gene82976:CDS | 40.0% | |
| ! | ACTAGGTGGTGATTTTGCTA+AGG | - | chr4.2:26860536-26860555 | MS.gene82976:CDS | 40.0% |
| ! | AGCTGCTGATTATCTGCATA+AGG | - | chr4.2:26860416-26860435 | MS.gene82976:CDS | 40.0% |
| ! | TTGGATGAACAGAAAGGTGT+AGG | - | chr4.2:26860378-26860397 | MS.gene82976:CDS | 40.0% |
| GATGATGTTGCTAAGCCTGA+AGG | - | chr4.2:26860498-26860517 | MS.gene82976:CDS | 45.0% | |
| GGTGATCTTCTAGATGCAGT+TGG | - | chr4.2:26860342-26860361 | MS.gene82976:CDS | 45.0% | |
| TGAGCTTATGGCAAGTGCAA+AGG | - | chr4.2:26860251-26860270 | MS.gene82976:CDS | 45.0% | |
| TGATCTGCAGGTTTAGAAGC+TGG | + | chr4.2:26860468-26860487 | None:intergenic | 45.0% | |
| ! | GGTGATTTTGCTAAGGTTGC+TGG | - | chr4.2:26860543-26860562 | MS.gene82976:CDS | 45.0% |
| !! | TCACTTTTAGGCTGATCTGC+AGG | + | chr4.2:26860480-26860499 | None:intergenic | 45.0% |
| AAGTGCAAAGGTGGTAGCAG+AGG | - | chr4.2:26860263-26860282 | MS.gene82976:CDS | 50.0% | |
| GATGTTGCTAAGCCTGAAGG+TGG | - | chr4.2:26860501-26860520 | MS.gene82976:CDS | 50.0% | |
| GCTTATGGCAAGTGCAAAGG+TGG | - | chr4.2:26860254-26860273 | MS.gene82976:CDS | 50.0% | |
| TCTGCAGGTTTAGAAGCTGG+TGG | + | chr4.2:26860465-26860484 | None:intergenic | 50.0% | |
| TGCACTTGCCATAAGCTCAC+TGG | + | chr4.2:26860250-26860269 | None:intergenic | 50.0% | |
| AAGCCTGAAGGTGGAGGATC+AGG | - | chr4.2:26860510-26860529 | MS.gene82976:CDS | 55.0% | |
| AGGCAGCACAGTCAGGTTTC+GGG | - | chr4.2:26860283-26860302 | MS.gene82976:CDS | 55.0% | |
| GACAAGACAGCAGAAGCTGC+AGG | - | chr4.2:26860321-26860340 | MS.gene82976:CDS | 55.0% | |
| GAGGCAGCACAGTCAGGTTT+CGG | - | chr4.2:26860282-26860301 | MS.gene82976:CDS | 55.0% | |
| GTTGCTAAGCCTGAAGGTGG+AGG | - | chr4.2:26860504-26860523 | MS.gene82976:CDS | 55.0% | |
| CCACCTGATCCTCCACCTTC+AGG | + | chr4.2:26860516-26860535 | None:intergenic | 60.0% | |
| CCTGAAGGTGGAGGATCAGG+TGG | - | chr4.2:26860513-26860532 | MS.gene82976:CDS | 60.0% | |
| GTAGCAGAGGCAGCACAGTC+AGG | - | chr4.2:26860276-26860295 | MS.gene82976:CDS | 60.0% | |
| ! | GGTGGAGGATCAGGTGGACT+AGG | - | chr4.2:26860519-26860538 | MS.gene82976:CDS | 60.0% |
| !! | GGAGGATCAGGTGGACTAGG+TGG | - | chr4.2:26860522-26860541 | MS.gene82976:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26860213 | 26860567 | 26860213 | ID=MS.gene82976 |
| chr4.2 | mRNA | 26860213 | 26860567 | 26860213 | ID=MS.gene82976.t1;Parent=MS.gene82976 |
| chr4.2 | exon | 26860213 | 26860567 | 26860213 | ID=MS.gene82976.t1.exon1;Parent=MS.gene82976.t1 |
| chr4.2 | CDS | 26860213 | 26860567 | 26860213 | ID=cds.MS.gene82976.t1;Parent=MS.gene82976.t1 |
| Gene Sequence |
| Protein sequence |
