Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82352.t1 | XP_003607781.1 | 92 | 125 | 7 | 1 | 1 | 122 | 1 | 125 | 2.30E-51 | 211.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82352.t1 | Q9ZQ80 | 55.9 | 68 | 27 | 1 | 15 | 79 | 68 | 135 | 4.4e-11 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82352.t1 | G7JGC9 | 92.0 | 125 | 7 | 1 | 1 | 122 | 1 | 125 | 1.6e-51 | 211.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene82352 | 0.815764 | 7.82E-52 | -1.69E-46 |
| MS.gene04917 | MS.gene82352 | 0.811618 | 6.40E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene82352 | 0.829236 | 5.76E-55 | -1.69E-46 |
| MS.gene049438 | MS.gene82352 | 0.824326 | 8.58E-54 | -1.69E-46 |
| MS.gene04991 | MS.gene82352 | -0.811173 | 7.99E-51 | -1.69E-46 |
| MS.gene050317 | MS.gene82352 | 0.801631 | 8.22E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene82352 | -0.829771 | 4.27E-55 | -1.69E-46 |
| MS.gene050629 | MS.gene82352 | -0.801712 | 7.91E-49 | -1.69E-46 |
| MS.gene050762 | MS.gene82352 | 0.826109 | 3.25E-54 | -1.69E-46 |
| MS.gene050763 | MS.gene82352 | 0.827563 | 1.46E-54 | -1.69E-46 |
| MS.gene05119 | MS.gene82352 | -0.801254 | 9.82E-49 | -1.69E-46 |
| MS.gene051245 | MS.gene82352 | 0.800998 | 1.11E-48 | -1.69E-46 |
| MS.gene051535 | MS.gene82352 | 0.812168 | 4.86E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene82352 | 0.819993 | 8.67E-53 | -1.69E-46 |
| MS.gene052302 | MS.gene82352 | 0.810204 | 1.29E-50 | -1.69E-46 |
| MS.gene052309 | MS.gene82352 | -0.847083 | 1.45E-59 | -1.69E-46 |
| MS.gene052376 | MS.gene82352 | 0.813331 | 2.70E-51 | -1.69E-46 |
| MS.gene053216 | MS.gene82352 | 0.812212 | 4.75E-51 | -1.69E-46 |
| MS.gene054379 | MS.gene82352 | 0.831627 | 1.50E-55 | -1.69E-46 |
| MS.gene054536 | MS.gene82352 | 0.800554 | 1.37E-48 | -1.69E-46 |
| MS.gene054930 | MS.gene82352 | 0.844213 | 8.69E-59 | -1.69E-46 |
| MS.gene055063 | MS.gene82352 | -0.800005 | 1.77E-48 | -1.69E-46 |
| MS.gene055441 | MS.gene82352 | 0.824192 | 9.22E-54 | -1.69E-46 |
| MS.gene055701 | MS.gene82352 | 0.825337 | 4.95E-54 | -1.69E-46 |
| MS.gene056087 | MS.gene82352 | 0.800793 | 1.22E-48 | -1.69E-46 |
| MS.gene056464 | MS.gene82352 | -0.803584 | 3.25E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene82352 | 0.83609 | 1.15E-56 | -1.69E-46 |
| MS.gene057451 | MS.gene82352 | -0.82365 | 1.24E-53 | -1.69E-46 |
| MS.gene057452 | MS.gene82352 | -0.832439 | 9.45E-56 | -1.69E-46 |
| MS.gene05761 | MS.gene82352 | 0.833531 | 5.05E-56 | -1.69E-46 |
| MS.gene058182 | MS.gene82352 | 0.823767 | 1.16E-53 | -1.69E-46 |
| MS.gene059872 | MS.gene82352 | -0.835992 | 1.21E-56 | -1.69E-46 |
| MS.gene060084 | MS.gene82352 | 0.868426 | 6.59E-66 | -1.69E-46 |
| MS.gene060297 | MS.gene82352 | 0.855974 | 4.40E-62 | -1.69E-46 |
| MS.gene060869 | MS.gene82352 | 0.847385 | 1.20E-59 | -1.69E-46 |
| MS.gene060870 | MS.gene82352 | 0.832681 | 8.23E-56 | -1.69E-46 |
| MS.gene060940 | MS.gene82352 | 0.834976 | 2.19E-56 | -1.69E-46 |
| MS.gene061002 | MS.gene82352 | 0.811448 | 6.96E-51 | -1.69E-46 |
| MS.gene061004 | MS.gene82352 | 0.826163 | 3.15E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82352.t1 | MTR_4g082860 | 91.200 | 125 | 8 | 1 | 1 | 122 | 1 | 125 | 7.04e-68 | 200 |
| MS.gene82352.t1 | MTR_4g082830 | 66.667 | 132 | 31 | 4 | 3 | 122 | 11 | 141 | 2.22e-40 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82352.t1 | AT2G03440 | 42.636 | 129 | 52 | 5 | 11 | 122 | 64 | 187 | 4.59e-14 | 65.5 |
| MS.gene82352.t1 | AT1G13930 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.21e-13 | 63.2 |
| MS.gene82352.t1 | AT1G13930 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.21e-13 | 63.2 |
| MS.gene82352.t1 | AT1G13930 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.21e-13 | 63.2 |
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCAACCAGTGAGCTTA+TGG | 0.299153 | 4.2:-26822789 | MS.gene82352:CDS |
| AGGCAGCACAGTCAGGTTTC+GGG | 0.304942 | 4.2:-26822745 | MS.gene82352:CDS |
| GAGGCAGCACAGTCAGGTTT+CGG | 0.309235 | 4.2:-26822746 | MS.gene82352:CDS |
| CCACCTGATCCTCCACCTTC+AGG | 0.341637 | 4.2:+26822515 | None:intergenic |
| AGTTGGTCAGTATGCTAAAT+TGG | 0.398975 | 4.2:-26822669 | MS.gene82352:CDS |
| AAGCCTGAAGGTGGAGGATC+AGG | 0.423090 | 4.2:-26822518 | MS.gene82352:CDS |
| TGCACTTGCCATAAGCTCAC+TGG | 0.466727 | 4.2:+26822781 | None:intergenic |
| AGCTGCTGATTATCTGCATA+AGG | 0.469713 | 4.2:-26822612 | MS.gene82352:CDS |
| GGTGGAGGATCAGGTGGACT+AGG | 0.476662 | 4.2:-26822509 | MS.gene82352:CDS |
| GACAAGACAGCAGAAGCTGC+AGG | 0.506059 | 4.2:-26822707 | MS.gene82352:CDS |
| GTAGCAGAGGCAGCACAGTC+AGG | 0.527765 | 4.2:-26822752 | MS.gene82352:CDS |
| TGATCTGCAGGTTTAGAAGC+TGG | 0.532370 | 4.2:+26822563 | None:intergenic |
| AAGGTTGAGAACACTACTAC+TGG | 0.547092 | 4.2:-26822593 | MS.gene82352:CDS |
| AAGTGCAAAGGTGGTAGCAG+AGG | 0.549500 | 4.2:-26822765 | MS.gene82352:CDS |
| TTGGATGAACAGAAAGGTGT+AGG | 0.558648 | 4.2:-26822650 | MS.gene82352:CDS |
| GCTAAATTGGATGAACAGAA+AGG | 0.567227 | 4.2:-26822656 | MS.gene82352:CDS |
| CCTGAAGGTGGAGGATCAGG+TGG | 0.601078 | 4.2:-26822515 | MS.gene82352:CDS |
| TGAGCTTATGGCAAGTGCAA+AGG | 0.606848 | 4.2:-26822777 | MS.gene82352:CDS |
| GGAGGATCAGGTGGACTAGG+TGG | 0.611533 | 4.2:-26822506 | MS.gene82352:CDS |
| GCTTATGGCAAGTGCAAAGG+TGG | 0.622253 | 4.2:-26822774 | MS.gene82352:CDS |
| GGTGATCTTCTAGATGCAGT+TGG | 0.625884 | 4.2:-26822686 | MS.gene82352:CDS |
| GTTGCTAAGCCTGAAGGTGG+AGG | 0.637775 | 4.2:-26822524 | MS.gene82352:CDS |
| TCTGCAGGTTTAGAAGCTGG+TGG | 0.662750 | 4.2:+26822566 | None:intergenic |
| GATGATGTTGCTAAGCCTGA+AGG | 0.667542 | 4.2:-26822530 | MS.gene82352:CDS |
| GATGTTGCTAAGCCTGAAGG+TGG | 0.674394 | 4.2:-26822527 | MS.gene82352:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGCAACATCATCACTTTT+AGG | + | chr4.2:26822748-26822767 | None:intergenic | 30.0% |
| AGTTGGTCAGTATGCTAAAT+TGG | - | chr4.2:26822615-26822634 | MS.gene82352:CDS | 35.0% | |
| GCTAAATTGGATGAACAGAA+AGG | - | chr4.2:26822628-26822647 | MS.gene82352:CDS | 35.0% | |
| AAGGTTGAGAACACTACTAC+TGG | - | chr4.2:26822691-26822710 | MS.gene82352:CDS | 40.0% | |
| GATTTCAACCAGTGAGCTTA+TGG | - | chr4.2:26822495-26822514 | MS.gene82352:CDS | 40.0% | |
| ! | ACTAGGTGGTGATTTTGCTA+AGG | - | chr4.2:26822792-26822811 | MS.gene82352:CDS | 40.0% |
| ! | AGCTGCTGATTATCTGCATA+AGG | - | chr4.2:26822672-26822691 | MS.gene82352:CDS | 40.0% |
| ! | TTGGATGAACAGAAAGGTGT+AGG | - | chr4.2:26822634-26822653 | MS.gene82352:CDS | 40.0% |
| GATGATGTTGCTAAGCCTGA+AGG | - | chr4.2:26822754-26822773 | MS.gene82352:CDS | 45.0% | |
| GGTGATCTTCTAGATGCAGT+TGG | - | chr4.2:26822598-26822617 | MS.gene82352:CDS | 45.0% | |
| TGAGCTTATGGCAAGTGCAA+AGG | - | chr4.2:26822507-26822526 | MS.gene82352:CDS | 45.0% | |
| TGATCTGCAGGTTTAGAAGC+TGG | + | chr4.2:26822724-26822743 | None:intergenic | 45.0% | |
| ! | GATTTTGCTAAGGTTGCTGG+AGG | - | chr4.2:26822802-26822821 | MS.gene82352:CDS | 45.0% |
| ! | GGTGATTTTGCTAAGGTTGC+TGG | - | chr4.2:26822799-26822818 | MS.gene82352:CDS | 45.0% |
| !! | TCACTTTTAGGCTGATCTGC+AGG | + | chr4.2:26822736-26822755 | None:intergenic | 45.0% |
| AAGTGCAAAGGTGGTAGCAG+AGG | - | chr4.2:26822519-26822538 | MS.gene82352:CDS | 50.0% | |
| GATGTTGCTAAGCCTGAAGG+TGG | - | chr4.2:26822757-26822776 | MS.gene82352:CDS | 50.0% | |
| GCTTATGGCAAGTGCAAAGG+TGG | - | chr4.2:26822510-26822529 | MS.gene82352:CDS | 50.0% | |
| TCTGCAGGTTTAGAAGCTGG+TGG | + | chr4.2:26822721-26822740 | None:intergenic | 50.0% | |
| TGCACTTGCCATAAGCTCAC+TGG | + | chr4.2:26822506-26822525 | None:intergenic | 50.0% | |
| AAGCCTGAAGGTGGAGGATC+AGG | - | chr4.2:26822766-26822785 | MS.gene82352:CDS | 55.0% | |
| AGGCAGCACAGTCAGGTTTC+GGG | - | chr4.2:26822539-26822558 | MS.gene82352:CDS | 55.0% | |
| GACAAGACAGCAGAAGCTGC+AGG | - | chr4.2:26822577-26822596 | MS.gene82352:CDS | 55.0% | |
| GAGGCAGCACAGTCAGGTTT+CGG | - | chr4.2:26822538-26822557 | MS.gene82352:CDS | 55.0% | |
| GTTGCTAAGCCTGAAGGTGG+AGG | - | chr4.2:26822760-26822779 | MS.gene82352:CDS | 55.0% | |
| CCACCTGATCCTCCACCTTC+AGG | + | chr4.2:26822772-26822791 | None:intergenic | 60.0% | |
| CCTGAAGGTGGAGGATCAGG+TGG | - | chr4.2:26822769-26822788 | MS.gene82352:CDS | 60.0% | |
| GTAGCAGAGGCAGCACAGTC+AGG | - | chr4.2:26822532-26822551 | MS.gene82352:CDS | 60.0% | |
| ! | GGTGGAGGATCAGGTGGACT+AGG | - | chr4.2:26822775-26822794 | MS.gene82352:CDS | 60.0% |
| !! | GGAGGATCAGGTGGACTAGG+TGG | - | chr4.2:26822778-26822797 | MS.gene82352:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26822469 | 26822837 | 26822469 | ID=MS.gene82352 |
| chr4.2 | mRNA | 26822469 | 26822837 | 26822469 | ID=MS.gene82352.t1;Parent=MS.gene82352 |
| chr4.2 | exon | 26822469 | 26822837 | 26822469 | ID=MS.gene82352.t1.exon1;Parent=MS.gene82352.t1 |
| chr4.2 | CDS | 26822469 | 26822837 | 26822469 | ID=cds.MS.gene82352.t1;Parent=MS.gene82352.t1 |
| Gene Sequence |
| Protein sequence |
