Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82978.t1 | XP_003607781.1 | 92 | 125 | 7 | 1 | 1 | 122 | 1 | 125 | 2.30E-51 | 211.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82978.t1 | Q9ZQ80 | 55.9 | 68 | 27 | 1 | 15 | 79 | 68 | 135 | 4.4e-11 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82978.t1 | G7JGC9 | 92.0 | 125 | 7 | 1 | 1 | 122 | 1 | 125 | 1.6e-51 | 211.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene82978 | 0.81915 | 1.35E-52 | -1.69E-46 |
| MS.gene04917 | MS.gene82978 | 0.814516 | 1.48E-51 | -1.69E-46 |
| MS.gene049230 | MS.gene82978 | 0.809269 | 2.06E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene82978 | 0.838698 | 2.46E-57 | -1.69E-46 |
| MS.gene049438 | MS.gene82978 | 0.837011 | 6.68E-57 | -1.69E-46 |
| MS.gene04991 | MS.gene82978 | -0.823714 | 1.19E-53 | -1.69E-46 |
| MS.gene050317 | MS.gene82978 | 0.812141 | 4.92E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene82978 | -0.832133 | 1.12E-55 | -1.69E-46 |
| MS.gene050629 | MS.gene82978 | -0.802853 | 4.61E-49 | -1.69E-46 |
| MS.gene050762 | MS.gene82978 | 0.831943 | 1.25E-55 | -1.69E-46 |
| MS.gene050763 | MS.gene82978 | 0.833408 | 5.43E-56 | -1.69E-46 |
| MS.gene05119 | MS.gene82978 | -0.800362 | 1.49E-48 | -1.69E-46 |
| MS.gene051535 | MS.gene82978 | 0.807819 | 4.19E-50 | -1.69E-46 |
| MS.gene051983 | MS.gene82978 | 0.801698 | 7.96E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene82978 | 0.831259 | 1.85E-55 | -1.69E-46 |
| MS.gene052302 | MS.gene82978 | 0.812183 | 4.82E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene82978 | -0.847489 | 1.12E-59 | -1.69E-46 |
| MS.gene052376 | MS.gene82978 | 0.813655 | 2.29E-51 | -1.69E-46 |
| MS.gene053216 | MS.gene82978 | 0.820631 | 6.19E-53 | -1.69E-46 |
| MS.gene05344 | MS.gene82978 | -0.814377 | 1.59E-51 | -1.69E-46 |
| MS.gene054379 | MS.gene82978 | 0.833879 | 4.14E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene82978 | 0.856034 | 4.23E-62 | -1.69E-46 |
| MS.gene055174 | MS.gene82978 | 0.806372 | 8.49E-50 | -1.69E-46 |
| MS.gene055441 | MS.gene82978 | 0.830667 | 2.58E-55 | -1.69E-46 |
| MS.gene055701 | MS.gene82978 | 0.819564 | 1.09E-52 | -1.69E-46 |
| MS.gene056087 | MS.gene82978 | 0.801693 | 7.98E-49 | -1.69E-46 |
| MS.gene056464 | MS.gene82978 | -0.811022 | 8.62E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene82978 | 0.839734 | 1.33E-57 | -1.69E-46 |
| MS.gene057451 | MS.gene82978 | -0.812675 | 3.76E-51 | -1.69E-46 |
| MS.gene057452 | MS.gene82978 | -0.820919 | 5.32E-53 | -1.69E-46 |
| MS.gene05761 | MS.gene82978 | 0.841887 | 3.62E-58 | -1.69E-46 |
| MS.gene058182 | MS.gene82978 | 0.833381 | 5.51E-56 | -1.69E-46 |
| MS.gene059687 | MS.gene82978 | 0.800361 | 1.49E-48 | -1.69E-46 |
| MS.gene059872 | MS.gene82978 | -0.835803 | 1.36E-56 | -1.69E-46 |
| MS.gene060084 | MS.gene82978 | 0.886285 | 3.91E-72 | -1.69E-46 |
| MS.gene060297 | MS.gene82978 | 0.863906 | 1.78E-64 | -1.69E-46 |
| MS.gene060869 | MS.gene82978 | 0.852241 | 5.25E-61 | -1.69E-46 |
| MS.gene060870 | MS.gene82978 | 0.82848 | 8.78E-55 | -1.69E-46 |
| MS.gene060940 | MS.gene82978 | 0.85078 | 1.36E-60 | -1.69E-46 |
| MS.gene060942 | MS.gene82978 | 0.804582 | 2.02E-49 | -1.69E-46 |
| MS.gene060943 | MS.gene82978 | 0.806466 | 8.11E-50 | -1.69E-46 |
| MS.gene061002 | MS.gene82978 | 0.822291 | 2.56E-53 | -1.69E-46 |
| MS.gene061004 | MS.gene82978 | 0.830053 | 3.65E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82978.t1 | MTR_4g082860 | 91.200 | 125 | 8 | 1 | 1 | 122 | 1 | 125 | 7.04e-68 | 200 |
| MS.gene82978.t1 | MTR_4g082830 | 66.667 | 132 | 31 | 4 | 3 | 122 | 11 | 141 | 2.22e-40 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82978.t1 | AT2G03440 | 42.636 | 129 | 52 | 5 | 11 | 122 | 64 | 187 | 4.59e-14 | 65.5 |
| MS.gene82978.t1 | AT1G13930 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.21e-13 | 63.2 |
| MS.gene82978.t1 | AT1G13930 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.21e-13 | 63.2 |
| MS.gene82978.t1 | AT1G13930 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.21e-13 | 63.2 |
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCAACCAGTGAGCTTA+TGG | 0.299153 | 4.2:-26849309 | MS.gene82978:CDS |
| AGGCAGCACAGTCAGGTTTC+GGG | 0.304942 | 4.2:-26849265 | MS.gene82978:CDS |
| GAGGCAGCACAGTCAGGTTT+CGG | 0.309235 | 4.2:-26849266 | MS.gene82978:CDS |
| CCACCTGATCCTCCACCTTC+AGG | 0.341637 | 4.2:+26849035 | None:intergenic |
| AGTTGGTCAGTATGCTAAAT+TGG | 0.398975 | 4.2:-26849189 | MS.gene82978:CDS |
| AAGCCTGAAGGTGGAGGATC+AGG | 0.423090 | 4.2:-26849038 | MS.gene82978:CDS |
| TGCACTTGCCATAAGCTCAC+TGG | 0.466727 | 4.2:+26849301 | None:intergenic |
| AGCTGCTGATTATCTGCATA+AGG | 0.469713 | 4.2:-26849132 | MS.gene82978:CDS |
| GGTGGAGGATCAGGTGGACT+AGG | 0.476662 | 4.2:-26849029 | MS.gene82978:CDS |
| GACAAGACAGCAGAAGCTGC+AGG | 0.506059 | 4.2:-26849227 | MS.gene82978:CDS |
| GTAGCAGAGGCAGCACAGTC+AGG | 0.527765 | 4.2:-26849272 | MS.gene82978:CDS |
| TGATCTGCAGGTTTAGAAGC+TGG | 0.532370 | 4.2:+26849083 | None:intergenic |
| AAGGTTGAGAACACTACTAC+TGG | 0.547092 | 4.2:-26849113 | MS.gene82978:CDS |
| AAGTGCAAAGGTGGTAGCAG+AGG | 0.549500 | 4.2:-26849285 | MS.gene82978:CDS |
| TTGGATGAACAGAAAGGTGT+AGG | 0.558648 | 4.2:-26849170 | MS.gene82978:CDS |
| GCTAAATTGGATGAACAGAA+AGG | 0.567227 | 4.2:-26849176 | MS.gene82978:CDS |
| CCTGAAGGTGGAGGATCAGG+TGG | 0.601078 | 4.2:-26849035 | MS.gene82978:CDS |
| TGAGCTTATGGCAAGTGCAA+AGG | 0.606848 | 4.2:-26849297 | MS.gene82978:CDS |
| GGAGGATCAGGTGGACTAGG+TGG | 0.611533 | 4.2:-26849026 | MS.gene82978:CDS |
| GCTTATGGCAAGTGCAAAGG+TGG | 0.622253 | 4.2:-26849294 | MS.gene82978:CDS |
| GGTGATCTTCTAGATGCAGT+TGG | 0.625884 | 4.2:-26849206 | MS.gene82978:CDS |
| GTTGCTAAGCCTGAAGGTGG+AGG | 0.637775 | 4.2:-26849044 | MS.gene82978:CDS |
| TCTGCAGGTTTAGAAGCTGG+TGG | 0.662750 | 4.2:+26849086 | None:intergenic |
| GATGATGTTGCTAAGCCTGA+AGG | 0.667542 | 4.2:-26849050 | MS.gene82978:CDS |
| GATGTTGCTAAGCCTGAAGG+TGG | 0.674394 | 4.2:-26849047 | MS.gene82978:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGCAACATCATCACTTTT+AGG | + | chr4.2:26849268-26849287 | None:intergenic | 30.0% |
| AGTTGGTCAGTATGCTAAAT+TGG | - | chr4.2:26849135-26849154 | MS.gene82978:CDS | 35.0% | |
| GCTAAATTGGATGAACAGAA+AGG | - | chr4.2:26849148-26849167 | MS.gene82978:CDS | 35.0% | |
| AAGGTTGAGAACACTACTAC+TGG | - | chr4.2:26849211-26849230 | MS.gene82978:CDS | 40.0% | |
| GATTTCAACCAGTGAGCTTA+TGG | - | chr4.2:26849015-26849034 | MS.gene82978:CDS | 40.0% | |
| ! | ACTAGGTGGTGATTTTGCTA+AGG | - | chr4.2:26849312-26849331 | MS.gene82978:CDS | 40.0% |
| ! | AGCTGCTGATTATCTGCATA+AGG | - | chr4.2:26849192-26849211 | MS.gene82978:CDS | 40.0% |
| ! | TTGGATGAACAGAAAGGTGT+AGG | - | chr4.2:26849154-26849173 | MS.gene82978:CDS | 40.0% |
| GATGATGTTGCTAAGCCTGA+AGG | - | chr4.2:26849274-26849293 | MS.gene82978:CDS | 45.0% | |
| GGTGATCTTCTAGATGCAGT+TGG | - | chr4.2:26849118-26849137 | MS.gene82978:CDS | 45.0% | |
| TGAGCTTATGGCAAGTGCAA+AGG | - | chr4.2:26849027-26849046 | MS.gene82978:CDS | 45.0% | |
| TGATCTGCAGGTTTAGAAGC+TGG | + | chr4.2:26849244-26849263 | None:intergenic | 45.0% | |
| ! | GATTTTGCTAAGGTTGCTGG+AGG | - | chr4.2:26849322-26849341 | MS.gene82978:CDS | 45.0% |
| ! | GGTGATTTTGCTAAGGTTGC+TGG | - | chr4.2:26849319-26849338 | MS.gene82978:CDS | 45.0% |
| !! | TCACTTTTAGGCTGATCTGC+AGG | + | chr4.2:26849256-26849275 | None:intergenic | 45.0% |
| AAGTGCAAAGGTGGTAGCAG+AGG | - | chr4.2:26849039-26849058 | MS.gene82978:CDS | 50.0% | |
| GATGTTGCTAAGCCTGAAGG+TGG | - | chr4.2:26849277-26849296 | MS.gene82978:CDS | 50.0% | |
| GCTTATGGCAAGTGCAAAGG+TGG | - | chr4.2:26849030-26849049 | MS.gene82978:CDS | 50.0% | |
| TCTGCAGGTTTAGAAGCTGG+TGG | + | chr4.2:26849241-26849260 | None:intergenic | 50.0% | |
| TGCACTTGCCATAAGCTCAC+TGG | + | chr4.2:26849026-26849045 | None:intergenic | 50.0% | |
| AAGCCTGAAGGTGGAGGATC+AGG | - | chr4.2:26849286-26849305 | MS.gene82978:CDS | 55.0% | |
| AGGCAGCACAGTCAGGTTTC+GGG | - | chr4.2:26849059-26849078 | MS.gene82978:CDS | 55.0% | |
| GACAAGACAGCAGAAGCTGC+AGG | - | chr4.2:26849097-26849116 | MS.gene82978:CDS | 55.0% | |
| GAGGCAGCACAGTCAGGTTT+CGG | - | chr4.2:26849058-26849077 | MS.gene82978:CDS | 55.0% | |
| GTTGCTAAGCCTGAAGGTGG+AGG | - | chr4.2:26849280-26849299 | MS.gene82978:CDS | 55.0% | |
| CCACCTGATCCTCCACCTTC+AGG | + | chr4.2:26849292-26849311 | None:intergenic | 60.0% | |
| CCTGAAGGTGGAGGATCAGG+TGG | - | chr4.2:26849289-26849308 | MS.gene82978:CDS | 60.0% | |
| GTAGCAGAGGCAGCACAGTC+AGG | - | chr4.2:26849052-26849071 | MS.gene82978:CDS | 60.0% | |
| ! | GGTGGAGGATCAGGTGGACT+AGG | - | chr4.2:26849295-26849314 | MS.gene82978:CDS | 60.0% |
| !! | GGAGGATCAGGTGGACTAGG+TGG | - | chr4.2:26849298-26849317 | MS.gene82978:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26848989 | 26849357 | 26848989 | ID=MS.gene82978 |
| chr4.2 | mRNA | 26848989 | 26849357 | 26848989 | ID=MS.gene82978.t1;Parent=MS.gene82978 |
| chr4.2 | exon | 26848989 | 26849357 | 26848989 | ID=MS.gene82978.t1.exon1;Parent=MS.gene82978.t1 |
| chr4.2 | CDS | 26848989 | 26849357 | 26848989 | ID=cds.MS.gene82978.t1;Parent=MS.gene82978.t1 |
| Gene Sequence |
| Protein sequence |
