Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44721.t1 | P30074.1 | 100 | 303 | 0 | 0 | 1 | 303 | 87 | 389 | 5.40E-171 | 610.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44721.t1 | P30074 | 100.0 | 303 | 0 | 0 | 1 | 303 | 87 | 389 | 1.3e-173 | 610.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44721.t1 | G7KR31 | 99.7 | 303 | 1 | 0 | 1 | 303 | 87 | 389 | 1.1e-170 | 608.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene44721 | -0.809265 | 2.06E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene44721 | -0.806797 | 6.91E-50 | -1.69E-46 |
| MS.gene049222 | MS.gene44721 | -0.812604 | 3.90E-51 | -1.69E-46 |
| MS.gene049232 | MS.gene44721 | -0.84794 | 8.41E-60 | -1.69E-46 |
| MS.gene049285 | MS.gene44721 | -0.803133 | 4.03E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene44721 | -0.810215 | 1.29E-50 | -1.69E-46 |
| MS.gene049483 | MS.gene44721 | 0.804906 | 1.73E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene44721 | 0.817599 | 3.03E-52 | -1.69E-46 |
| MS.gene049627 | MS.gene44721 | -0.801071 | 1.07E-48 | -1.69E-46 |
| MS.gene05010 | MS.gene44721 | -0.83321 | 6.08E-56 | -1.69E-46 |
| MS.gene050129 | MS.gene44721 | 0.800439 | 1.44E-48 | -1.69E-46 |
| MS.gene050170 | MS.gene44721 | -0.806387 | 8.43E-50 | -1.69E-46 |
| MS.gene050216 | MS.gene44721 | -0.841758 | 3.91E-58 | -1.69E-46 |
| MS.gene050323 | MS.gene44721 | -0.820693 | 5.99E-53 | -1.69E-46 |
| MS.gene050363 | MS.gene44721 | 0.823555 | 1.30E-53 | -1.69E-46 |
| MS.gene050380 | MS.gene44721 | 0.837665 | 4.55E-57 | -1.69E-46 |
| MS.gene050381 | MS.gene44721 | 0.894386 | 2.59E-75 | -1.69E-46 |
| MS.gene050496 | MS.gene44721 | -0.817349 | 3.45E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene44721 | -0.851004 | 1.18E-60 | -1.69E-46 |
| MS.gene050518 | MS.gene44721 | -0.800187 | 1.62E-48 | -1.69E-46 |
| MS.gene050577 | MS.gene44721 | -0.805337 | 1.40E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene44721 | -0.81316 | 2.95E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene44721 | -0.858964 | 5.74E-63 | -1.69E-46 |
| MS.gene050871 | MS.gene44721 | -0.861639 | 8.91E-64 | -1.69E-46 |
| MS.gene050883 | MS.gene44721 | 0.907302 | 5.87E-81 | -1.69E-46 |
| MS.gene050986 | MS.gene44721 | -0.81501 | 1.15E-51 | -1.69E-46 |
| MS.gene05104 | MS.gene44721 | 0.817908 | 2.58E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene44721 | 0.872387 | 3.31E-67 | -1.69E-46 |
| MS.gene051268 | MS.gene44721 | -0.811708 | 6.12E-51 | -1.69E-46 |
| MS.gene051364 | MS.gene44721 | 0.808364 | 3.21E-50 | -1.69E-46 |
| MS.gene05141 | MS.gene44721 | 0.889662 | 1.98E-73 | -1.69E-46 |
| MS.gene05142 | MS.gene44721 | 0.833476 | 5.22E-56 | -1.69E-46 |
| MS.gene051560 | MS.gene44721 | -0.819141 | 1.36E-52 | -1.69E-46 |
| MS.gene051694 | MS.gene44721 | -0.813289 | 2.76E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene44721 | 0.886099 | 4.59E-72 | -1.69E-46 |
| MS.gene052164 | MS.gene44721 | -0.82735 | 1.64E-54 | -1.69E-46 |
| MS.gene05234 | MS.gene44721 | 0.81812 | 2.31E-52 | -1.69E-46 |
| MS.gene052482 | MS.gene44721 | -0.83674 | 7.84E-57 | -1.69E-46 |
| MS.gene052486 | MS.gene44721 | -0.840148 | 1.04E-57 | -1.69E-46 |
| MS.gene052966 | MS.gene44721 | -0.824773 | 6.73E-54 | -1.69E-46 |
| MS.gene053069 | MS.gene44721 | 0.818244 | 2.17E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene44721 | -0.855458 | 6.22E-62 | -1.69E-46 |
| MS.gene053420 | MS.gene44721 | 0.854245 | 1.40E-61 | -1.69E-46 |
| MS.gene053549 | MS.gene44721 | 0.83847 | 2.82E-57 | -1.69E-46 |
| MS.gene053561 | MS.gene44721 | 0.832862 | 7.42E-56 | -1.69E-46 |
| MS.gene053574 | MS.gene44721 | -0.8161 | 6.58E-52 | -1.69E-46 |
| MS.gene053626 | MS.gene44721 | -0.820296 | 7.39E-53 | -1.69E-46 |
| MS.gene053826 | MS.gene44721 | 0.83734 | 5.51E-57 | -1.69E-46 |
| MS.gene054003 | MS.gene44721 | -0.83788 | 4.00E-57 | -1.69E-46 |
| MS.gene054595 | MS.gene44721 | -0.837749 | 4.33E-57 | -1.69E-46 |
| MS.gene054616 | MS.gene44721 | 0.802039 | 6.78E-49 | -1.69E-46 |
| MS.gene054707 | MS.gene44721 | 0.815743 | 7.90E-52 | -1.69E-46 |
| MS.gene054708 | MS.gene44721 | 0.853949 | 1.70E-61 | -1.69E-46 |
| MS.gene054954 | MS.gene44721 | -0.81636 | 5.76E-52 | -1.69E-46 |
| MS.gene055124 | MS.gene44721 | 0.849352 | 3.42E-60 | -1.69E-46 |
| MS.gene055139 | MS.gene44721 | 0.84081 | 6.95E-58 | -1.69E-46 |
| MS.gene055154 | MS.gene44721 | 0.846703 | 1.84E-59 | -1.69E-46 |
| MS.gene055202 | MS.gene44721 | 0.846133 | 2.63E-59 | -1.69E-46 |
| MS.gene055362 | MS.gene44721 | -0.814254 | 1.69E-51 | -1.69E-46 |
| MS.gene055401 | MS.gene44721 | 0.821912 | 3.14E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene44721 | -0.831335 | 1.77E-55 | -1.69E-46 |
| MS.gene055577 | MS.gene44721 | -0.832177 | 1.10E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene44721 | -0.818622 | 1.78E-52 | -1.69E-46 |
| MS.gene056166 | MS.gene44721 | -0.815095 | 1.10E-51 | -1.69E-46 |
| MS.gene056298 | MS.gene44721 | 0.806617 | 7.54E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene44721 | 0.919422 | 4.59E-87 | -1.69E-46 |
| MS.gene056311 | MS.gene44721 | 0.928728 | 1.92E-92 | -1.69E-46 |
| MS.gene056313 | MS.gene44721 | 0.930814 | 9.45E-94 | -1.69E-46 |
| MS.gene056408 | MS.gene44721 | 0.830554 | 2.75E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene44721 | -0.847983 | 8.19E-60 | -1.69E-46 |
| MS.gene056510 | MS.gene44721 | -0.822258 | 2.61E-53 | -1.69E-46 |
| MS.gene056550 | MS.gene44721 | 0.803387 | 3.57E-49 | -1.69E-46 |
| MS.gene056577 | MS.gene44721 | 0.802412 | 5.68E-49 | -1.69E-46 |
| MS.gene056878 | MS.gene44721 | -0.810748 | 9.88E-51 | -1.69E-46 |
| MS.gene057014 | MS.gene44721 | 0.884175 | 2.40E-71 | -1.69E-46 |
| MS.gene057173 | MS.gene44721 | 0.807761 | 4.32E-50 | -1.69E-46 |
| MS.gene057499 | MS.gene44721 | 0.824004 | 1.02E-53 | -1.69E-46 |
| MS.gene057506 | MS.gene44721 | 0.81139 | 7.17E-51 | -1.69E-46 |
| MS.gene058041 | MS.gene44721 | -0.809543 | 1.80E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene44721 | 0.816174 | 6.33E-52 | -1.69E-46 |
| MS.gene058108 | MS.gene44721 | 0.855698 | 5.30E-62 | -1.69E-46 |
| MS.gene058109 | MS.gene44721 | 0.882197 | 1.27E-70 | -1.69E-46 |
| MS.gene058111 | MS.gene44721 | 0.867628 | 1.19E-65 | -1.69E-46 |
| MS.gene058117 | MS.gene44721 | 0.809832 | 1.56E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene44721 | -0.85691 | 2.34E-62 | -1.69E-46 |
| MS.gene058170 | MS.gene44721 | 0.839712 | 1.34E-57 | -1.69E-46 |
| MS.gene058171 | MS.gene44721 | 0.903721 | 2.59E-79 | -1.69E-46 |
| MS.gene058172 | MS.gene44721 | 0.810742 | 9.91E-51 | -1.69E-46 |
| MS.gene058173 | MS.gene44721 | 0.910073 | 2.81E-82 | -1.69E-46 |
| MS.gene058174 | MS.gene44721 | 0.838055 | 3.61E-57 | -1.69E-46 |
| MS.gene058175 | MS.gene44721 | 0.909376 | 6.10E-82 | -1.69E-46 |
| MS.gene058179 | MS.gene44721 | 0.818264 | 2.15E-52 | -1.69E-46 |
| MS.gene058180 | MS.gene44721 | 0.820135 | 8.05E-53 | -1.69E-46 |
| MS.gene058183 | MS.gene44721 | -0.800424 | 1.45E-48 | -1.69E-46 |
| MS.gene05837 | MS.gene44721 | -0.84778 | 9.31E-60 | -1.69E-46 |
| MS.gene058493 | MS.gene44721 | 0.852986 | 3.22E-61 | -1.69E-46 |
| MS.gene058494 | MS.gene44721 | 0.855112 | 7.85E-62 | -1.69E-46 |
| MS.gene058496 | MS.gene44721 | 0.800238 | 1.58E-48 | -1.69E-46 |
| MS.gene05884 | MS.gene44721 | 0.806485 | 8.04E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene44721 | -0.832933 | 7.12E-56 | -1.69E-46 |
| MS.gene059023 | MS.gene44721 | -0.807649 | 4.56E-50 | -1.69E-46 |
| MS.gene059034 | MS.gene44721 | -0.808201 | 3.48E-50 | -1.69E-46 |
| MS.gene059037 | MS.gene44721 | 0.822032 | 2.94E-53 | -1.69E-46 |
| MS.gene059045 | MS.gene44721 | 0.842654 | 2.27E-58 | -1.69E-46 |
| MS.gene059052 | MS.gene44721 | 0.802828 | 4.66E-49 | -1.69E-46 |
| MS.gene059223 | MS.gene44721 | 0.854386 | 1.27E-61 | -1.69E-46 |
| MS.gene059236 | MS.gene44721 | 0.845739 | 3.37E-59 | -1.69E-46 |
| MS.gene059367 | MS.gene44721 | -0.826929 | 2.07E-54 | -1.69E-46 |
| MS.gene059718 | MS.gene44721 | -0.811281 | 7.57E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene44721 | -0.842064 | 3.25E-58 | -1.69E-46 |
| MS.gene06000 | MS.gene44721 | -0.800185 | 1.62E-48 | -1.69E-46 |
| MS.gene060187 | MS.gene44721 | 0.866937 | 1.98E-65 | -1.69E-46 |
| MS.gene060482 | MS.gene44721 | -0.843158 | 1.66E-58 | -1.69E-46 |
| MS.gene060595 | MS.gene44721 | 0.854295 | 1.35E-61 | -1.69E-46 |
| MS.gene060602 | MS.gene44721 | 0.810041 | 1.40E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene44721 | -0.801278 | 9.71E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene44721 | -0.8145 | 1.49E-51 | -1.69E-46 |
| MS.gene060778 | MS.gene44721 | -0.836725 | 7.91E-57 | -1.69E-46 |
| MS.gene06077 | MS.gene44721 | -0.865778 | 4.61E-65 | -1.69E-46 |
| MS.gene060982 | MS.gene44721 | -0.818152 | 2.28E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44721.t1 | MTR_7g016700 | 99.340 | 303 | 2 | 0 | 1 | 303 | 87 | 389 | 0.0 | 622 |
| MS.gene44721.t1 | MTR_7g016800 | 99.670 | 303 | 1 | 0 | 1 | 303 | 87 | 389 | 0.0 | 622 |
| MS.gene44721.t1 | MTR_7g016820 | 99.340 | 303 | 2 | 0 | 1 | 303 | 87 | 389 | 0.0 | 620 |
| MS.gene44721.t1 | MTR_7g016720 | 99.340 | 303 | 2 | 0 | 1 | 303 | 87 | 389 | 0.0 | 618 |
| MS.gene44721.t1 | MTR_1g098140 | 98.680 | 303 | 4 | 0 | 1 | 303 | 87 | 389 | 0.0 | 615 |
| MS.gene44721.t1 | MTR_7g016780 | 98.350 | 303 | 5 | 0 | 1 | 303 | 87 | 389 | 0.0 | 615 |
| MS.gene44721.t1 | MTR_5g007730 | 98.350 | 303 | 5 | 0 | 1 | 303 | 87 | 389 | 0.0 | 615 |
| MS.gene44721.t1 | MTR_1g097935 | 98.350 | 303 | 5 | 0 | 1 | 303 | 87 | 389 | 0.0 | 613 |
| MS.gene44721.t1 | MTR_1g097910 | 98.020 | 303 | 6 | 0 | 1 | 303 | 87 | 389 | 0.0 | 613 |
| MS.gene44721.t1 | MTR_5g007760 | 96.700 | 303 | 10 | 0 | 1 | 303 | 87 | 389 | 0.0 | 606 |
| MS.gene44721.t1 | MTR_5g007717 | 95.710 | 303 | 13 | 0 | 1 | 303 | 87 | 389 | 0.0 | 602 |
| MS.gene44721.t1 | MTR_5g007713 | 96.040 | 303 | 12 | 0 | 1 | 303 | 87 | 389 | 0.0 | 602 |
| MS.gene44721.t1 | MTR_7g113405 | 96.370 | 303 | 11 | 0 | 1 | 303 | 1 | 303 | 0.0 | 597 |
| MS.gene44721.t1 | MTR_3g083920 | 92.053 | 302 | 24 | 0 | 1 | 302 | 87 | 388 | 0.0 | 582 |
| MS.gene44721.t1 | MTR_3g083910 | 91.060 | 302 | 27 | 0 | 1 | 302 | 87 | 388 | 0.0 | 580 |
| MS.gene44721.t1 | MTR_2g058470 | 87.459 | 303 | 38 | 0 | 1 | 303 | 87 | 389 | 0.0 | 553 |
| MS.gene44721.t1 | MTR_7g084300 | 83.168 | 303 | 51 | 0 | 1 | 303 | 87 | 389 | 0.0 | 531 |
| MS.gene44721.t1 | MTR_5g007740 | 82.392 | 301 | 53 | 0 | 3 | 303 | 26 | 326 | 0.0 | 516 |
| MS.gene44721.t1 | MTR_3g086260 | 80.795 | 302 | 58 | 0 | 1 | 302 | 87 | 388 | 0.0 | 513 |
| MS.gene44721.t1 | MTR_3g088675 | 68.771 | 301 | 94 | 0 | 3 | 303 | 89 | 389 | 1.46e-163 | 460 |
| MS.gene44721.t1 | MTR_5g007770 | 77.660 | 282 | 30 | 2 | 2 | 283 | 19 | 267 | 1.55e-155 | 435 |
| MS.gene44721.t1 | MTR_5g007723 | 71.947 | 303 | 57 | 2 | 1 | 303 | 124 | 398 | 1.37e-153 | 435 |
| MS.gene44721.t1 | MTR_3g088670 | 49.175 | 303 | 145 | 4 | 9 | 302 | 86 | 388 | 1.35e-103 | 308 |
| MS.gene44721.t1 | MTR_4g075560 | 43.092 | 304 | 167 | 3 | 2 | 302 | 89 | 389 | 3.65e-84 | 258 |
| MS.gene44721.t1 | MTR_4g078730 | 38.127 | 299 | 179 | 1 | 3 | 301 | 97 | 389 | 6.31e-78 | 242 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44721.t1 | AT5G13930 | 85.197 | 304 | 44 | 1 | 1 | 303 | 92 | 395 | 0.0 | 537 |
| MS.gene44721.t1 | AT4G00040 | 43.234 | 303 | 163 | 4 | 2 | 301 | 89 | 385 | 1.24e-80 | 249 |
| MS.gene44721.t1 | AT4G34850 | 41.275 | 298 | 172 | 2 | 8 | 303 | 23 | 319 | 9.20e-80 | 245 |
| MS.gene44721.t1 | AT4G34850 | 41.275 | 298 | 172 | 2 | 8 | 303 | 96 | 392 | 4.94e-79 | 245 |
| MS.gene44721.t1 | AT1G02050 | 41.254 | 303 | 169 | 4 | 2 | 301 | 96 | 392 | 1.14e-72 | 229 |
| MS.gene44721.t1 | AT3G52160 | 26.556 | 241 | 161 | 9 | 64 | 301 | 97 | 324 | 3.58e-12 | 66.6 |
| MS.gene44721.t1 | AT3G52160 | 26.556 | 241 | 161 | 9 | 64 | 301 | 210 | 437 | 8.97e-12 | 65.9 |
Find 80 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATAGGTTTCTCAATTTC+TGG | 0.117134 | 7.2:-81139085 | None:intergenic |
| CTTGAATGGGGTGTACTATT+TGG | 0.210123 | 7.2:+81139490 | MS.gene44721:CDS |
| CTGATTACAACTCAATCTTT+TGG | 0.293179 | 7.2:+81139275 | MS.gene44721:CDS |
| AGAGAAGTGCTCAGTGAATA+TGG | 0.300231 | 7.2:+81139379 | MS.gene44721:CDS |
| AACCTATATTTGAGATGGTT+TGG | 0.308691 | 7.2:+81139098 | MS.gene44721:CDS |
| TCCACTTGATCTAGAATTGC+AGG | 0.315800 | 7.2:-81139316 | None:intergenic |
| TGGGGTGTACTATTTGGTTT+TGG | 0.329196 | 7.2:+81139496 | MS.gene44721:CDS |
| TATCAACTCACCAAACTCTT+AGG | 0.335572 | 7.2:+81138821 | MS.gene44721:CDS |
| GCTTCGTTTGGCTAAAGATT+TGG | 0.342769 | 7.2:+81138907 | MS.gene44721:CDS |
| GGTTTCTCAATTTCTGGTAC+TGG | 0.354846 | 7.2:-81139079 | None:intergenic |
| TCAATGGCTCCTTCACTATC+AGG | 0.368167 | 7.2:-81139139 | None:intergenic |
| AAGTGAGTAATCTTTGACTT+TGG | 0.381814 | 7.2:-81138755 | None:intergenic |
| CTTGTTGGACAAGCACTATT+CGG | 0.382146 | 7.2:+81139022 | MS.gene44721:CDS |
| CACCTTCTTAAAGATGTTCC+TGG | 0.385004 | 7.2:+81139193 | MS.gene44721:CDS |
| TGAATATATGGCACCTTCCT+TGG | 0.393161 | 7.2:+81138649 | None:intergenic |
| GAGTTTGGTGAGTTGATAAT+CGG | 0.404976 | 7.2:-81138816 | None:intergenic |
| AGGTGGCACGGTGCTTCGTT+TGG | 0.407834 | 7.2:+81138895 | MS.gene44721:CDS |
| GGAGCTGCTGCACTCATTGT+TGG | 0.411529 | 7.2:+81139049 | MS.gene44721:CDS |
| AACTGGAGAAGGACTTGAAT+GGG | 0.424547 | 7.2:+81139477 | MS.gene44721:CDS |
| CACCTGCAAAACACCCTTGT+TGG | 0.425276 | 7.2:-81138877 | None:intergenic |
| ACTCACTTGGACAGTCTTGT+TGG | 0.427238 | 7.2:+81139007 | MS.gene44721:CDS |
| GGTTGAGGCTTTCGAACCAT+TGG | 0.433432 | 7.2:+81139246 | MS.gene44721:CDS |
| ACGTAAAACAACTGTTTCAA+TGG | 0.433787 | 7.2:-81139530 | None:intergenic |
| GTCCAAACCATCTCAAATAT+AGG | 0.437103 | 7.2:-81139100 | None:intergenic |
| GTCACCGCTGTCACATTTCG+TGG | 0.448537 | 7.2:+81138974 | MS.gene44721:CDS |
| TGGGCGAAGACCTAAGAGTT+TGG | 0.450749 | 7.2:-81138831 | None:intergenic |
| AAATAATTAAACATCTCATA+TGG | 0.456479 | 7.2:-81139560 | None:intergenic |
| ATCTAGAATTGCAGGTCCAC+CGG | 0.463612 | 7.2:-81139308 | None:intergenic |
| CAAAACGGATTGAAGACAAC+TGG | 0.465367 | 7.2:+81139460 | MS.gene44721:CDS |
| AGTGAAGGCTATAAAAGAAT+GGG | 0.468743 | 7.2:+81138727 | MS.gene44721:CDS |
| TTCACGAAGGTGACCATCAA+TGG | 0.471758 | 7.2:-81139155 | None:intergenic |
| TGGCACCTTCCTTGGATGCT+AGG | 0.472685 | 7.2:+81138657 | MS.gene44721:CDS |
| GCAAGACATGGTGGTGGTAG+AGG | 0.474656 | 7.2:+81138679 | MS.gene44721:CDS |
| AAAGAACATCACTAAAGCAT+TGG | 0.475145 | 7.2:+81139225 | MS.gene44721:CDS |
| GTTGAGGCTTTCGAACCATT+GGG | 0.476179 | 7.2:+81139247 | MS.gene44721:CDS |
| ACCTTCTTAAAGATGTTCCT+GGG | 0.484500 | 7.2:+81139194 | MS.gene44721:CDS |
| GATGGTCACCTTCGTGAAGC+TGG | 0.486168 | 7.2:+81139160 | MS.gene44721:CDS |
| GATAGTGAAGGAGCCATTGA+TGG | 0.488582 | 7.2:+81139142 | MS.gene44721:CDS |
| CAGTGAAGGCTATAAAAGAA+TGG | 0.502364 | 7.2:+81138726 | MS.gene44721:CDS |
| GTCTTCGCCCATATGTGAAA+AGG | 0.504788 | 7.2:+81138843 | MS.gene44721:CDS |
| TGAGAAACCTATATTTGAGA+TGG | 0.507221 | 7.2:+81139093 | MS.gene44721:CDS |
| AGAAAGAAATCAACTCAAAA+CGG | 0.511986 | 7.2:+81139445 | MS.gene44721:CDS |
| GTGAGTTGATAATCGGCTCC+AGG | 0.515720 | 7.2:-81138809 | None:intergenic |
| CAACTGGAGAAGGACTTGAA+TGG | 0.524308 | 7.2:+81139476 | MS.gene44721:CDS |
| CATGTCTTGCCTAGCATCCA+AGG | 0.526806 | 7.2:-81138666 | None:intergenic |
| AGGGAAGGAGGCTGCAGTGA+AGG | 0.537534 | 7.2:+81138712 | MS.gene44721:CDS |
| ATGTTCTTTGAAACAATCCC+AGG | 0.543601 | 7.2:-81139211 | None:intergenic |
| TCCCAGGAACATCTTTAAGA+AGG | 0.550142 | 7.2:-81139195 | None:intergenic |
| GTGGTAGAGGTACCTAGACT+AGG | 0.557229 | 7.2:+81138692 | MS.gene44721:CDS |
| CTGTCCAAGTGAGTATCACT+AGG | 0.558628 | 7.2:-81138998 | None:intergenic |
| ACCTGCAATTCTAGATCAAG+TGG | 0.566779 | 7.2:+81139315 | MS.gene44721:CDS |
| AGGCATGTCTACACCACTTG+TGG | 0.569916 | 7.2:-81138789 | None:intergenic |
| TCTAGAATTGCAGGTCCACC+GGG | 0.570884 | 7.2:-81139307 | None:intergenic |
| ACAAGTGGTGTAGACATGCC+TGG | 0.572074 | 7.2:+81138791 | MS.gene44721:CDS |
| GCAGCCTCCTTCCCTAGTCT+AGG | 0.573798 | 7.2:-81138704 | None:intergenic |
| GGATTGAAGACAACTGGAGA+AGG | 0.579947 | 7.2:+81139466 | MS.gene44721:CDS |
| CATCACTAAAGCATTGGTTG+AGG | 0.580186 | 7.2:+81139231 | MS.gene44721:CDS |
| TTAATCGTTTGCACCACAAG+TGG | 0.591579 | 7.2:+81138776 | MS.gene44721:CDS |
| TCACCGCTGTCACATTTCGT+GGG | 0.591938 | 7.2:+81138975 | MS.gene44721:CDS |
| GGACAAGCACTATTCGGAGA+TGG | 0.596263 | 7.2:+81139028 | MS.gene44721:CDS |
| TGTGTTGGTTGTTTGTTCTG+AGG | 0.596318 | 7.2:+81138952 | MS.gene44721:CDS |
| AGAGGTACCTAGACTAGGGA+AGG | 0.600145 | 7.2:+81138697 | MS.gene44721:CDS |
| ACTGTTTCAATGGTAAGTCC+CGG | 0.600220 | 7.2:-81139520 | None:intergenic |
| CTTGGATGCTAGGCAAGACA+TGG | 0.600480 | 7.2:+81138667 | MS.gene44721:CDS |
| TGGTAGAGGTACCTAGACTA+GGG | 0.618791 | 7.2:+81138693 | MS.gene44721:CDS |
| CAACAAAGGTGCTCGTGTGT+TGG | 0.620198 | 7.2:+81138937 | MS.gene44721:CDS |
| TGGGCCTAGTGATACTCACT+TGG | 0.622076 | 7.2:+81138994 | MS.gene44721:CDS |
| GGTACCTAGACTAGGGAAGG+AGG | 0.631813 | 7.2:+81138700 | MS.gene44721:CDS |
| TCTTGCCTAGCATCCAAGGA+AGG | 0.637347 | 7.2:-81138662 | None:intergenic |
| ACAATTGCTCCTGATAGTGA+AGG | 0.642382 | 7.2:+81139130 | MS.gene44721:CDS |
| TTGTAATCAGAAATTCCCAA+TGG | 0.649608 | 7.2:-81139262 | None:intergenic |
| GGTACATGATGTACCAACAA+GGG | 0.652620 | 7.2:+81138864 | MS.gene44721:CDS |
| TGCTAGGCAAGACATGGTGG+TGG | 0.675229 | 7.2:+81138673 | MS.gene44721:CDS |
| AGGTACATGATGTACCAACA+AGG | 0.675679 | 7.2:+81138863 | MS.gene44721:CDS |
| GTGAAGGCTATAAAAGAATG+GGG | 0.681481 | 7.2:+81138728 | MS.gene44721:CDS |
| GATTTGGCTGAGAACAACAA+AGG | 0.683242 | 7.2:+81138923 | MS.gene44721:CDS |
| ATGTTAGTCCAGCTTCACGA+AGG | 0.698828 | 7.2:-81139168 | None:intergenic |
| GGATGCTAGGCAAGACATGG+TGG | 0.713925 | 7.2:+81138670 | MS.gene44721:CDS |
| ACTGGAGAAGGACTTGAATG+GGG | 0.717086 | 7.2:+81139478 | MS.gene44721:CDS |
| AGGCCCACGAAATGTGACAG+CGG | 0.766137 | 7.2:-81138978 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATATAGGTTTCTCAATTTC+TGG | - | chr7.2:81139088-81139107 | None:intergenic | 25.0% |
| ! | AGAAAGAAATCAACTCAAAA+CGG | + | chr7.2:81139445-81139464 | MS.gene44721:CDS | 25.0% |
| !!! | ATTCATCTTTTCAGGTTTTA+AGG | - | chr7.2:81139353-81139372 | None:intergenic | 25.0% |
| AAAGAACATCACTAAAGCAT+TGG | + | chr7.2:81139225-81139244 | MS.gene44721:CDS | 30.0% | |
| ACGTAAAACAACTGTTTCAA+TGG | - | chr7.2:81139533-81139552 | None:intergenic | 30.0% | |
| AGTGAAGGCTATAAAAGAAT+GGG | + | chr7.2:81138727-81138746 | MS.gene44721:CDS | 30.0% | |
| TGAGAAACCTATATTTGAGA+TGG | + | chr7.2:81139093-81139112 | MS.gene44721:CDS | 30.0% | |
| TTGTAATCAGAAATTCCCAA+TGG | - | chr7.2:81139265-81139284 | None:intergenic | 30.0% | |
| ! | AACCTATATTTGAGATGGTT+TGG | + | chr7.2:81139098-81139117 | MS.gene44721:CDS | 30.0% |
| ! | AAGTGAGTAATCTTTGACTT+TGG | - | chr7.2:81138758-81138777 | None:intergenic | 30.0% |
| ! | ATCATGTACCTTTTCACATA+TGG | - | chr7.2:81138854-81138873 | None:intergenic | 30.0% |
| ! | CTGATTACAACTCAATCTTT+TGG | + | chr7.2:81139275-81139294 | MS.gene44721:CDS | 30.0% |
| ! | TCATGTACCTTTTCACATAT+GGG | - | chr7.2:81138853-81138872 | None:intergenic | 30.0% |
| !!! | TGCATGTGTTTTGTTTATCT+TGG | + | chr7.2:81139414-81139433 | MS.gene44721:CDS | 30.0% |
| ACCTTCTTAAAGATGTTCCT+GGG | + | chr7.2:81139194-81139213 | MS.gene44721:CDS | 35.0% | |
| CAGTGAAGGCTATAAAAGAA+TGG | + | chr7.2:81138726-81138745 | MS.gene44721:CDS | 35.0% | |
| GTCCAAACCATCTCAAATAT+AGG | - | chr7.2:81139103-81139122 | None:intergenic | 35.0% | |
| GTGAAGGCTATAAAAGAATG+GGG | + | chr7.2:81138728-81138747 | MS.gene44721:CDS | 35.0% | |
| TATCAACTCACCAAACTCTT+AGG | + | chr7.2:81138821-81138840 | MS.gene44721:CDS | 35.0% | |
| ! | CTAGTTGCATTCATCTTTTC+AGG | - | chr7.2:81139361-81139380 | None:intergenic | 35.0% |
| !! | AACAGTTGTTTTACGTAGTG+TGG | + | chr7.2:81139537-81139556 | MS.gene44721:CDS | 35.0% |
| !! | ATGTTCTTTGAAACAATCCC+AGG | - | chr7.2:81139214-81139233 | None:intergenic | 35.0% |
| !! | GAGTTTGGTGAGTTGATAAT+CGG | - | chr7.2:81138819-81138838 | None:intergenic | 35.0% |
| !!! | TGTACTATTTGGTTTTGGAC+CGG | + | chr7.2:81139501-81139520 | MS.gene44721:CDS | 35.0% |
| AACTGGAGAAGGACTTGAAT+GGG | + | chr7.2:81139477-81139496 | MS.gene44721:CDS | 40.0% | |
| ACCTGCAATTCTAGATCAAG+TGG | + | chr7.2:81139315-81139334 | MS.gene44721:CDS | 40.0% | |
| ACTGTTTCAATGGTAAGTCC+CGG | - | chr7.2:81139523-81139542 | None:intergenic | 40.0% | |
| AGGTACATGATGTACCAACA+AGG | + | chr7.2:81138863-81138882 | MS.gene44721:CDS | 40.0% | |
| CACCTTCTTAAAGATGTTCC+TGG | + | chr7.2:81139193-81139212 | MS.gene44721:CDS | 40.0% | |
| CTTGAATGGGGTGTACTATT+TGG | + | chr7.2:81139490-81139509 | MS.gene44721:CDS | 40.0% | |
| CTTGTTGGACAAGCACTATT+CGG | + | chr7.2:81139022-81139041 | MS.gene44721:CDS | 40.0% | |
| GATTTGGCTGAGAACAACAA+AGG | + | chr7.2:81138923-81138942 | MS.gene44721:CDS | 40.0% | |
| GCTTCGTTTGGCTAAAGATT+TGG | + | chr7.2:81138907-81138926 | MS.gene44721:CDS | 40.0% | |
| GGTACATGATGTACCAACAA+GGG | + | chr7.2:81138864-81138883 | MS.gene44721:CDS | 40.0% | |
| TTAATCGTTTGCACCACAAG+TGG | + | chr7.2:81138776-81138795 | MS.gene44721:CDS | 40.0% | |
| ! | ACAATTGCTCCTGATAGTGA+AGG | + | chr7.2:81139130-81139149 | MS.gene44721:CDS | 40.0% |
| ! | ATCTTTTGGATTGCACATCC+CGG | + | chr7.2:81139289-81139308 | MS.gene44721:CDS | 40.0% |
| ! | TCCACTTGATCTAGAATTGC+AGG | - | chr7.2:81139319-81139338 | None:intergenic | 40.0% |
| ! | TCCCAGGAACATCTTTAAGA+AGG | - | chr7.2:81139198-81139217 | None:intergenic | 40.0% |
| !! | AGAGAAGTGCTCAGTGAATA+TGG | + | chr7.2:81139379-81139398 | MS.gene44721:CDS | 40.0% |
| !! | CAAAACGGATTGAAGACAAC+TGG | + | chr7.2:81139460-81139479 | MS.gene44721:CDS | 40.0% |
| !! | CATCACTAAAGCATTGGTTG+AGG | + | chr7.2:81139231-81139250 | MS.gene44721:CDS | 40.0% |
| !! | GGTTTCTCAATTTCTGGTAC+TGG | - | chr7.2:81139082-81139101 | None:intergenic | 40.0% |
| !! | TGGGGTGTACTATTTGGTTT+TGG | + | chr7.2:81139496-81139515 | MS.gene44721:CDS | 40.0% |
| !! | TGTGTTGGTTGTTTGTTCTG+AGG | + | chr7.2:81138952-81138971 | MS.gene44721:CDS | 40.0% |
| !!! | GTACTATTTGGTTTTGGACC+GGG | + | chr7.2:81139502-81139521 | MS.gene44721:CDS | 40.0% |
| ACTGGAGAAGGACTTGAATG+GGG | + | chr7.2:81139478-81139497 | MS.gene44721:CDS | 45.0% | |
| ATGTTAGTCCAGCTTCACGA+AGG | - | chr7.2:81139171-81139190 | None:intergenic | 45.0% | |
| CAACTGGAGAAGGACTTGAA+TGG | + | chr7.2:81139476-81139495 | MS.gene44721:CDS | 45.0% | |
| CTGTCCAAGTGAGTATCACT+AGG | - | chr7.2:81139001-81139020 | None:intergenic | 45.0% | |
| GATAGTGAAGGAGCCATTGA+TGG | + | chr7.2:81139142-81139161 | MS.gene44721:CDS | 45.0% | |
| GGATTGAAGACAACTGGAGA+AGG | + | chr7.2:81139466-81139485 | MS.gene44721:CDS | 45.0% | |
| GTCTTCGCCCATATGTGAAA+AGG | + | chr7.2:81138843-81138862 | MS.gene44721:CDS | 45.0% | |
| GTTGAGGCTTTCGAACCATT+GGG | + | chr7.2:81139247-81139266 | MS.gene44721:CDS | 45.0% | |
| TCAATGGCTCCTTCACTATC+AGG | - | chr7.2:81139142-81139161 | None:intergenic | 45.0% | |
| TTCACGAAGGTGACCATCAA+TGG | - | chr7.2:81139158-81139177 | None:intergenic | 45.0% | |
| ! | ACTCACTTGGACAGTCTTGT+TGG | + | chr7.2:81139007-81139026 | MS.gene44721:CDS | 45.0% |
| ! | ATCTAGAATTGCAGGTCCAC+CGG | - | chr7.2:81139311-81139330 | None:intergenic | 45.0% |
| !! | TGGTAGAGGTACCTAGACTA+GGG | + | chr7.2:81138693-81138712 | MS.gene44721:CDS | 45.0% |
| !!! | TACCAACAAGGGTGTTTTGC+AGG | + | chr7.2:81138875-81138894 | MS.gene44721:CDS | 45.0% |
| ACAAGTGGTGTAGACATGCC+TGG | + | chr7.2:81138791-81138810 | MS.gene44721:CDS | 50.0% | |
| AGGCATGTCTACACCACTTG+TGG | - | chr7.2:81138792-81138811 | None:intergenic | 50.0% | |
| CACCTGCAAAACACCCTTGT+TGG | - | chr7.2:81138880-81138899 | None:intergenic | 50.0% | |
| CATGTCTTGCCTAGCATCCA+AGG | - | chr7.2:81138669-81138688 | None:intergenic | 50.0% | |
| CTTGGATGCTAGGCAAGACA+TGG | + | chr7.2:81138667-81138686 | MS.gene44721:CDS | 50.0% | |
| GGACAAGCACTATTCGGAGA+TGG | + | chr7.2:81139028-81139047 | MS.gene44721:CDS | 50.0% | |
| GGTTGAGGCTTTCGAACCAT+TGG | + | chr7.2:81139246-81139265 | MS.gene44721:CDS | 50.0% | |
| TCACCGCTGTCACATTTCGT+GGG | + | chr7.2:81138975-81138994 | MS.gene44721:CDS | 50.0% | |
| TCTTGCCTAGCATCCAAGGA+AGG | - | chr7.2:81138665-81138684 | None:intergenic | 50.0% | |
| TGGGCCTAGTGATACTCACT+TGG | + | chr7.2:81138994-81139013 | MS.gene44721:CDS | 50.0% | |
| TGGGCGAAGACCTAAGAGTT+TGG | - | chr7.2:81138834-81138853 | None:intergenic | 50.0% | |
| ! | TCTAGAATTGCAGGTCCACC+GGG | - | chr7.2:81139310-81139329 | None:intergenic | 50.0% |
| ! | TTTTGGATTGCACATCCCGG+TGG | + | chr7.2:81139292-81139311 | MS.gene44721:CDS | 50.0% |
| !! | AGAGGTACCTAGACTAGGGA+AGG | + | chr7.2:81138697-81138716 | MS.gene44721:CDS | 50.0% |
| !! | CAACAAAGGTGCTCGTGTGT+TGG | + | chr7.2:81138937-81138956 | MS.gene44721:CDS | 50.0% |
| !! | GTGAGTTGATAATCGGCTCC+AGG | - | chr7.2:81138812-81138831 | None:intergenic | 50.0% |
| !! | GTGGTAGAGGTACCTAGACT+AGG | + | chr7.2:81138692-81138711 | MS.gene44721:CDS | 50.0% |
| !!! | CAACAAGGGTGTTTTGCAGG+TGG | + | chr7.2:81138878-81138897 | MS.gene44721:CDS | 50.0% |
| AGGCCCACGAAATGTGACAG+CGG | - | chr7.2:81138981-81139000 | None:intergenic | 55.0% | |
| GATGGTCACCTTCGTGAAGC+TGG | + | chr7.2:81139160-81139179 | MS.gene44721:CDS | 55.0% | |
| GGAGCTGCTGCACTCATTGT+TGG | + | chr7.2:81139049-81139068 | MS.gene44721:CDS | 55.0% | |
| GGATGCTAGGCAAGACATGG+TGG | + | chr7.2:81138670-81138689 | MS.gene44721:CDS | 55.0% | |
| GGTACCTAGACTAGGGAAGG+AGG | + | chr7.2:81138700-81138719 | MS.gene44721:CDS | 55.0% | |
| GTCACCGCTGTCACATTTCG+TGG | + | chr7.2:81138974-81138993 | MS.gene44721:CDS | 55.0% | |
| TGCTAGGCAAGACATGGTGG+TGG | + | chr7.2:81138673-81138692 | MS.gene44721:CDS | 55.0% | |
| TGGCACCTTCCTTGGATGCT+AGG | + | chr7.2:81138657-81138676 | MS.gene44721:CDS | 55.0% | |
| !! | AGGGTGTTTTGCAGGTGGCA+CGG | + | chr7.2:81138883-81138902 | MS.gene44721:CDS | 55.0% |
| !! | GCAAGACATGGTGGTGGTAG+AGG | + | chr7.2:81138679-81138698 | MS.gene44721:CDS | 55.0% |
| AGGGAAGGAGGCTGCAGTGA+AGG | + | chr7.2:81138712-81138731 | MS.gene44721:CDS | 60.0% | |
| AGGTGGCACGGTGCTTCGTT+TGG | + | chr7.2:81138895-81138914 | MS.gene44721:CDS | 60.0% | |
| GCAGCCTCCTTCCCTAGTCT+AGG | - | chr7.2:81138707-81138726 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 81138656 | 81139567 | 81138656 | ID=MS.gene44721 |
| chr7.2 | mRNA | 81138656 | 81139567 | 81138656 | ID=MS.gene44721.t1;Parent=MS.gene44721 |
| chr7.2 | exon | 81138656 | 81139567 | 81138656 | ID=MS.gene44721.t1.exon1;Parent=MS.gene44721.t1 |
| chr7.2 | CDS | 81138656 | 81139567 | 81138656 | ID=cds.MS.gene44721.t1;Parent=MS.gene44721.t1 |
| Gene Sequence |
| Protein sequence |
