Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44720.t1 | P30074.1 | 99.5 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 1.50E-221 | 778.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44720.t1 | P30074 | 99.5 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 3.5e-224 | 778.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44720.t1 | G7KR27 | 99.0 | 389 | 4 | 0 | 1 | 389 | 1 | 389 | 2.0e-220 | 774.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene44720 | -0.804377 | 2.22E-49 | -1.69E-46 |
| MS.gene049312 | MS.gene44720 | 0.806786 | 6.95E-50 | -1.69E-46 |
| MS.gene049573 | MS.gene44720 | 0.800677 | 1.29E-48 | -1.69E-46 |
| MS.gene05010 | MS.gene44720 | -0.805284 | 1.44E-49 | -1.69E-46 |
| MS.gene050129 | MS.gene44720 | 0.80574 | 1.15E-49 | -1.69E-46 |
| MS.gene050216 | MS.gene44720 | -0.802744 | 4.85E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene44720 | 0.836122 | 1.13E-56 | -1.69E-46 |
| MS.gene050380 | MS.gene44720 | 0.812337 | 4.46E-51 | -1.69E-46 |
| MS.gene050381 | MS.gene44720 | 0.886432 | 3.44E-72 | -1.69E-46 |
| MS.gene050504 | MS.gene44720 | -0.828761 | 7.51E-55 | -1.69E-46 |
| MS.gene050868 | MS.gene44720 | -0.83269 | 8.19E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene44720 | -0.83553 | 1.59E-56 | -1.69E-46 |
| MS.gene050883 | MS.gene44720 | 0.907807 | 3.40E-81 | -1.69E-46 |
| MS.gene051127 | MS.gene44720 | 0.866401 | 2.93E-65 | -1.69E-46 |
| MS.gene05141 | MS.gene44720 | 0.87797 | 4.10E-69 | -1.69E-46 |
| MS.gene05142 | MS.gene44720 | 0.815366 | 9.59E-52 | -1.69E-46 |
| MS.gene051946 | MS.gene44720 | 0.882401 | 1.07E-70 | -1.69E-46 |
| MS.gene052486 | MS.gene44720 | -0.800981 | 1.12E-48 | -1.69E-46 |
| MS.gene053069 | MS.gene44720 | 0.826454 | 2.69E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene44720 | -0.81515 | 1.07E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene44720 | 0.833393 | 5.47E-56 | -1.69E-46 |
| MS.gene053549 | MS.gene44720 | 0.851032 | 1.16E-60 | -1.69E-46 |
| MS.gene053561 | MS.gene44720 | 0.828251 | 9.97E-55 | -1.69E-46 |
| MS.gene053826 | MS.gene44720 | 0.831501 | 1.61E-55 | -1.69E-46 |
| MS.gene054595 | MS.gene44720 | -0.813375 | 2.64E-51 | -1.69E-46 |
| MS.gene054708 | MS.gene44720 | 0.843656 | 1.23E-58 | -1.69E-46 |
| MS.gene055124 | MS.gene44720 | 0.857514 | 1.55E-62 | -1.69E-46 |
| MS.gene055139 | MS.gene44720 | 0.84059 | 7.93E-58 | -1.69E-46 |
| MS.gene055154 | MS.gene44720 | 0.843849 | 1.09E-58 | -1.69E-46 |
| MS.gene055202 | MS.gene44720 | 0.808462 | 3.06E-50 | -1.69E-46 |
| MS.gene055401 | MS.gene44720 | 0.843008 | 1.82E-58 | -1.69E-46 |
| MS.gene055502 | MS.gene44720 | -0.800157 | 1.64E-48 | -1.69E-46 |
| MS.gene055577 | MS.gene44720 | -0.801692 | 7.99E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene44720 | 0.930367 | 1.82E-93 | -1.69E-46 |
| MS.gene056311 | MS.gene44720 | 0.918034 | 2.56E-86 | -1.69E-46 |
| MS.gene056313 | MS.gene44720 | 0.928524 | 2.56E-92 | -1.69E-46 |
| MS.gene056408 | MS.gene44720 | 0.847467 | 1.14E-59 | -1.69E-46 |
| MS.gene056504 | MS.gene44720 | -0.813252 | 2.81E-51 | -1.69E-46 |
| MS.gene057014 | MS.gene44720 | 0.874886 | 4.76E-68 | -1.69E-46 |
| MS.gene057506 | MS.gene44720 | 0.814798 | 1.28E-51 | -1.69E-46 |
| MS.gene057770 | MS.gene44720 | 0.821495 | 3.92E-53 | -1.69E-46 |
| MS.gene058099 | MS.gene44720 | 0.820858 | 5.49E-53 | -1.69E-46 |
| MS.gene058108 | MS.gene44720 | 0.837196 | 5.99E-57 | -1.69E-46 |
| MS.gene058109 | MS.gene44720 | 0.865946 | 4.08E-65 | -1.69E-46 |
| MS.gene058111 | MS.gene44720 | 0.820959 | 5.21E-53 | -1.69E-46 |
| MS.gene058157 | MS.gene44720 | -0.818546 | 1.85E-52 | -1.69E-46 |
| MS.gene058170 | MS.gene44720 | 0.819777 | 9.72E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene44720 | 0.875392 | 3.19E-68 | -1.69E-46 |
| MS.gene058172 | MS.gene44720 | 0.804514 | 2.08E-49 | -1.69E-46 |
| MS.gene058173 | MS.gene44720 | 0.87268 | 2.64E-67 | -1.69E-46 |
| MS.gene058174 | MS.gene44720 | 0.817612 | 3.01E-52 | -1.69E-46 |
| MS.gene058175 | MS.gene44720 | 0.875738 | 2.43E-68 | -1.69E-46 |
| MS.gene058176 | MS.gene44720 | 0.806009 | 1.01E-49 | -1.69E-46 |
| MS.gene058180 | MS.gene44720 | 0.803531 | 3.34E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene44720 | -0.812187 | 4.81E-51 | -1.69E-46 |
| MS.gene058493 | MS.gene44720 | 0.820626 | 6.21E-53 | -1.69E-46 |
| MS.gene058494 | MS.gene44720 | 0.826969 | 2.03E-54 | -1.69E-46 |
| MS.gene058628 | MS.gene44720 | 0.806654 | 7.41E-50 | -1.69E-46 |
| MS.gene058629 | MS.gene44720 | 0.80481 | 1.81E-49 | -1.69E-46 |
| MS.gene058630 | MS.gene44720 | 0.819499 | 1.12E-52 | -1.69E-46 |
| MS.gene05884 | MS.gene44720 | 0.81502 | 1.15E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene44720 | -0.819034 | 1.44E-52 | -1.69E-46 |
| MS.gene059034 | MS.gene44720 | -0.800445 | 1.44E-48 | -1.69E-46 |
| MS.gene059037 | MS.gene44720 | 0.806923 | 6.50E-50 | -1.69E-46 |
| MS.gene059044 | MS.gene44720 | 0.817547 | 3.12E-52 | -1.69E-46 |
| MS.gene059045 | MS.gene44720 | 0.832077 | 1.16E-55 | -1.69E-46 |
| MS.gene059223 | MS.gene44720 | 0.857764 | 1.31E-62 | -1.69E-46 |
| MS.gene059236 | MS.gene44720 | 0.812337 | 4.46E-51 | -1.69E-46 |
| MS.gene059367 | MS.gene44720 | -0.80989 | 1.51E-50 | -1.69E-46 |
| MS.gene059697 | MS.gene44720 | 0.805889 | 1.07E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene44720 | -0.802424 | 5.65E-49 | -1.69E-46 |
| MS.gene060187 | MS.gene44720 | 0.827017 | 1.97E-54 | -1.69E-46 |
| MS.gene060482 | MS.gene44720 | -0.829933 | 3.90E-55 | -1.69E-46 |
| MS.gene060595 | MS.gene44720 | 0.809497 | 1.84E-50 | -1.69E-46 |
| MS.gene060602 | MS.gene44720 | 0.806834 | 6.78E-50 | -1.69E-46 |
| MS.gene060778 | MS.gene44720 | -0.809319 | 2.01E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene44720 | -0.842905 | 1.94E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44720.t1 | MTR_7g016820 | 98.715 | 389 | 5 | 0 | 1 | 389 | 1 | 389 | 0.0 | 799 |
| MS.gene44720.t1 | MTR_7g016700 | 98.458 | 389 | 6 | 0 | 1 | 389 | 1 | 389 | 0.0 | 799 |
| MS.gene44720.t1 | MTR_7g016720 | 98.972 | 389 | 4 | 0 | 1 | 389 | 1 | 389 | 0.0 | 799 |
| MS.gene44720.t1 | MTR_7g016800 | 98.715 | 389 | 5 | 0 | 1 | 389 | 1 | 389 | 0.0 | 798 |
| MS.gene44720.t1 | MTR_7g016780 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 795 |
| MS.gene44720.t1 | MTR_5g007730 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 795 |
| MS.gene44720.t1 | MTR_1g098140 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 793 |
| MS.gene44720.t1 | MTR_1g097935 | 97.943 | 389 | 8 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene44720.t1 | MTR_1g097910 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene44720.t1 | MTR_5g007760 | 96.401 | 389 | 14 | 0 | 1 | 389 | 1 | 389 | 0.0 | 783 |
| MS.gene44720.t1 | MTR_5g007717 | 96.144 | 389 | 15 | 0 | 1 | 389 | 1 | 389 | 0.0 | 782 |
| MS.gene44720.t1 | MTR_5g007713 | 96.144 | 389 | 15 | 0 | 1 | 389 | 1 | 389 | 0.0 | 781 |
| MS.gene44720.t1 | MTR_3g083920 | 90.206 | 388 | 38 | 0 | 1 | 388 | 1 | 388 | 0.0 | 746 |
| MS.gene44720.t1 | MTR_3g083910 | 89.433 | 388 | 41 | 0 | 1 | 388 | 1 | 388 | 0.0 | 744 |
| MS.gene44720.t1 | MTR_2g058470 | 87.147 | 389 | 50 | 0 | 1 | 389 | 1 | 389 | 0.0 | 717 |
| MS.gene44720.t1 | MTR_3g086260 | 81.701 | 388 | 71 | 0 | 1 | 388 | 1 | 388 | 0.0 | 679 |
| MS.gene44720.t1 | MTR_7g084300 | 80.977 | 389 | 74 | 0 | 1 | 389 | 1 | 389 | 0.0 | 677 |
| MS.gene44720.t1 | MTR_7g113405 | 96.040 | 303 | 12 | 0 | 87 | 389 | 1 | 303 | 0.0 | 598 |
| MS.gene44720.t1 | MTR_3g088675 | 66.838 | 389 | 129 | 0 | 1 | 389 | 1 | 389 | 0.0 | 582 |
| MS.gene44720.t1 | MTR_5g007740 | 80.368 | 326 | 64 | 0 | 64 | 389 | 1 | 326 | 0.0 | 549 |
| MS.gene44720.t1 | MTR_5g007723 | 68.514 | 397 | 76 | 5 | 12 | 389 | 32 | 398 | 0.0 | 531 |
| MS.gene44720.t1 | MTR_5g007770 | 78.000 | 300 | 33 | 2 | 70 | 369 | 1 | 267 | 2.49e-168 | 471 |
| MS.gene44720.t1 | MTR_3g088670 | 48.974 | 390 | 185 | 6 | 10 | 388 | 2 | 388 | 1.87e-130 | 380 |
| MS.gene44720.t1 | MTR_4g075560 | 42.632 | 380 | 211 | 4 | 11 | 387 | 13 | 388 | 2.00e-103 | 311 |
| MS.gene44720.t1 | MTR_4g078730 | 37.265 | 373 | 227 | 2 | 15 | 387 | 24 | 389 | 2.25e-92 | 283 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44720.t1 | AT5G13930 | 81.959 | 388 | 69 | 1 | 3 | 389 | 8 | 395 | 0.0 | 672 |
| MS.gene44720.t1 | AT4G34850 | 40.584 | 377 | 220 | 3 | 15 | 389 | 18 | 392 | 1.92e-97 | 296 |
| MS.gene44720.t1 | AT4G00040 | 39.541 | 392 | 225 | 6 | 1 | 387 | 1 | 385 | 4.39e-96 | 292 |
| MS.gene44720.t1 | AT1G02050 | 38.817 | 389 | 227 | 6 | 2 | 387 | 12 | 392 | 5.52e-87 | 269 |
| MS.gene44720.t1 | AT4G34850 | 40.000 | 320 | 189 | 2 | 72 | 389 | 1 | 319 | 6.57e-82 | 253 |
| MS.gene44720.t1 | AT3G52160 | 26.556 | 241 | 161 | 9 | 150 | 387 | 97 | 324 | 9.42e-12 | 66.2 |
| MS.gene44720.t1 | AT3G52160 | 26.556 | 241 | 161 | 9 | 150 | 387 | 210 | 437 | 2.24e-11 | 65.5 |
Find 101 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATAGGTTTCTCAATTTC+TGG | 0.117134 | 7.2:+81172512 | None:intergenic |
| CTTGAATGGGGTGTGTTATT+TGG | 0.274984 | 7.2:-81172107 | MS.gene44720:CDS |
| TGGGGTGTGTTATTTGGTTT+TGG | 0.280962 | 7.2:-81172101 | MS.gene44720:CDS |
| AAGTGAGTAATCTTTGATTT+TGG | 0.284045 | 7.2:+81172842 | None:intergenic |
| CCGATTACAACTCAATCTTT+TGG | 0.295351 | 7.2:-81172322 | MS.gene44720:CDS |
| AACCTATATTTGAGATGGTT+TGG | 0.308691 | 7.2:-81172499 | MS.gene44720:CDS |
| GTTGTTCTCAGCCAAATCTT+TGG | 0.312932 | 7.2:+81172679 | None:intergenic |
| CTTGTTGGACAAGCACTATT+TGG | 0.314421 | 7.2:-81172575 | MS.gene44720:CDS |
| TCCACTTGATCTAGAATTGC+AGG | 0.315800 | 7.2:+81172281 | None:intergenic |
| AGAGAAGTGCTCAGCGAGTA+TGG | 0.334012 | 7.2:-81172218 | MS.gene44720:CDS |
| TATCAACTCACCAAACTCTT+AGG | 0.335572 | 7.2:-81172776 | MS.gene44720:CDS |
| GCTTCGGTTGGCCAAAGATT+TGG | 0.349225 | 7.2:-81172690 | MS.gene44720:CDS |
| GGTTTCTCAATTTCTGGTAC+TGG | 0.354846 | 7.2:+81172518 | None:intergenic |
| GATGGTCACCTTCGTGAAGC+TGG | 0.367169 | 7.2:-81172437 | MS.gene44720:CDS |
| TCAATGGCTCCTTCACTATC+AGG | 0.368167 | 7.2:+81172458 | None:intergenic |
| CTTTGTTCAACACAATTTGC+AGG | 0.373711 | 7.2:+81173489 | None:intergenic |
| TGAATACATGGCCCCTTCAT+TGG | 0.380102 | 7.2:-81172948 | MS.gene44720:CDS |
| CACCTTCTTAAAGATGTTCC+TGG | 0.385004 | 7.2:-81172404 | MS.gene44720:CDS |
| CCAATGGCCAAGATAGTTGC+AGG | 0.390729 | 7.2:+81173522 | None:intergenic |
| GAGTTTGGTGAGTTGATAAT+CGG | 0.404976 | 7.2:+81172781 | None:intergenic |
| CCAAAAGATTGAGTTGTAAT+CGG | 0.409783 | 7.2:+81172322 | None:intergenic |
| AGAAGGCCCTGCAACTATCT+TGG | 0.418429 | 7.2:-81173529 | MS.gene44720:CDS |
| GCCATGTATTCACAAACATT+AGG | 0.420204 | 7.2:+81172959 | None:intergenic |
| CACCTGCAAAACACCCTTGT+TGG | 0.425276 | 7.2:+81172720 | None:intergenic |
| ACTCACTTGGACAGCCTTGT+TGG | 0.426712 | 7.2:-81172590 | MS.gene44720:CDS |
| TTGCAGGTGGCACGGTGCTT+CGG | 0.428855 | 7.2:-81172706 | MS.gene44720:CDS |
| GGTTGAGGCTTTCGAGCCAT+TGG | 0.428986 | 7.2:-81172351 | MS.gene44720:CDS |
| GGAGCTGCTGCACTCGTTGT+TGG | 0.430968 | 7.2:-81172548 | MS.gene44720:CDS |
| CAAGAAGGATTGAAGACAAC+AGG | 0.432547 | 7.2:-81172137 | MS.gene44720:CDS |
| GTCCAAACCATCTCAAATAT+AGG | 0.437103 | 7.2:+81172497 | None:intergenic |
| AAATAATTAAGCATTTCATA+TGG | 0.440442 | 7.2:+81172037 | None:intergenic |
| AACAGGAGAAGGACTTGAAT+GGG | 0.444037 | 7.2:-81172120 | MS.gene44720:CDS |
| TGGGCGAAGACCTAAGAGTT+TGG | 0.450749 | 7.2:+81172766 | None:intergenic |
| GCTGTCCAAGTGAGTATCAC+TGG | 0.458078 | 7.2:+81172598 | None:intergenic |
| ACGCAAAACAACCGTTTCAA+TGG | 0.461439 | 7.2:+81172067 | None:intergenic |
| ATCTAGAATTGCAGGTCCAC+CGG | 0.463612 | 7.2:+81172289 | None:intergenic |
| GCAAGACATGGTGGTGGTAG+AGG | 0.469468 | 7.2:-81172918 | MS.gene44720:CDS |
| CATGTATCTCCTCTTGATCA+TGG | 0.469562 | 7.2:+81173012 | None:intergenic |
| TTCACGAAGGTGACCATCAA+TGG | 0.471758 | 7.2:+81172442 | None:intergenic |
| AAAGAACATCACTAAAGCAT+TGG | 0.475145 | 7.2:-81172372 | MS.gene44720:CDS |
| GTTGAGGCTTTCGAGCCATT+GGG | 0.477937 | 7.2:-81172350 | MS.gene44720:CDS |
| TCTTGCCTTGCATCCAATGA+AGG | 0.479267 | 7.2:+81172935 | None:intergenic |
| GTCACCGCTGTCACATTTCG+TGG | 0.482260 | 7.2:-81172623 | MS.gene44720:CDS |
| CGGTGAAGGCTATAAAAGAA+TGG | 0.483789 | 7.2:-81172871 | MS.gene44720:CDS |
| ACCTTCTTAAAGATGTTCCT+GGG | 0.484500 | 7.2:-81172403 | MS.gene44720:CDS |
| CTGAAATTCGTAAAGCTCAA+AGG | 0.484509 | 7.2:-81173554 | MS.gene44720:CDS |
| TCCTAATGTTTGTGAATACA+TGG | 0.486149 | 7.2:-81172960 | MS.gene44720:CDS |
| GATAGTGAAGGAGCCATTGA+TGG | 0.488582 | 7.2:-81172455 | MS.gene44720:CDS |
| CAACAGGAGAAGGACTTGAA+TGG | 0.502457 | 7.2:-81172121 | MS.gene44720:CDS |
| GTCTTCGCCCATATGTGAAA+AGG | 0.504788 | 7.2:-81172754 | MS.gene44720:CDS |
| TGAGAAACCTATATTTGAGA+TGG | 0.507221 | 7.2:-81172504 | MS.gene44720:CDS |
| GGTGAAGGCTATAAAAGAAT+GGG | 0.509368 | 7.2:-81172870 | MS.gene44720:CDS |
| ACAAGTGGTGTCGACATGCC+TGG | 0.510677 | 7.2:-81172806 | MS.gene44720:CDS |
| ACCAGGACTTACCATTGAAA+CGG | 0.510913 | 7.2:-81172078 | MS.gene44720:CDS |
| TGAAATTCGTAAAGCTCAAA+GGG | 0.513791 | 7.2:-81173553 | MS.gene44720:CDS |
| GTGAGTTGATAATCGGCTCC+AGG | 0.515720 | 7.2:+81172788 | None:intergenic |
| TGCAGGATTTGCAGTGCCAA+TGG | 0.515817 | 7.2:+81173506 | None:intergenic |
| AGGGAAGGAGGCTGCGGTGA+AGG | 0.518723 | 7.2:-81172885 | MS.gene44720:CDS |
| AGGTGGCACGGTGCTTCGGT+TGG | 0.524604 | 7.2:-81172702 | MS.gene44720:CDS |
| CTGTCCAAGTGAGTATCACT+GGG | 0.525567 | 7.2:+81172599 | None:intergenic |
| ACCGTTTCAATGGTAAGTCC+TGG | 0.536054 | 7.2:+81172077 | None:intergenic |
| ATGGTCACCTTCGTGAAGCT+GGG | 0.540796 | 7.2:-81172436 | MS.gene44720:CDS |
| ATGTTCTTTGAAACAATCCC+AGG | 0.543601 | 7.2:+81172386 | None:intergenic |
| AGAAAGAAATCAACTCAAGA+AGG | 0.548629 | 7.2:-81172152 | MS.gene44720:CDS |
| TCCCAGGAACATCTTTAAGA+AGG | 0.550142 | 7.2:+81172402 | None:intergenic |
| GTGGTAGAGGTACCTAGACT+AGG | 0.557229 | 7.2:-81172905 | MS.gene44720:CDS |
| CTTGCCTTGCATCCAATGAA+GGG | 0.559841 | 7.2:+81172936 | None:intergenic |
| TGGCCCCTTCATTGGATGCA+AGG | 0.561644 | 7.2:-81172940 | MS.gene44720:CDS |
| GGACAAGCACTATTTGGAGA+TGG | 0.563405 | 7.2:-81172569 | MS.gene44720:CDS |
| CCTGCAACTATCTTGGCCAT+TGG | 0.566609 | 7.2:-81173522 | MS.gene44720:CDS |
| ACCTGCAATTCTAGATCAAG+TGG | 0.566779 | 7.2:-81172282 | MS.gene44720:CDS |
| TCTAGAATTGCAGGTCCACC+GGG | 0.570884 | 7.2:+81172290 | None:intergenic |
| CAAATAGTGCTTGTCCAACA+AGG | 0.572762 | 7.2:+81172576 | None:intergenic |
| GGATTGAAGACAACAGGAGA+AGG | 0.573266 | 7.2:-81172131 | MS.gene44720:CDS |
| AGGCATGTCGACACCACTTG+TGG | 0.578425 | 7.2:+81172808 | None:intergenic |
| CATCACTAAAGCATTGGTTG+AGG | 0.580186 | 7.2:-81172366 | MS.gene44720:CDS |
| GTGATAAATCCATGATCAAG+AGG | 0.584564 | 7.2:-81173021 | MS.gene44720:CDS |
| TTAATCGTTTGCACCACAAG+TGG | 0.591579 | 7.2:-81172821 | MS.gene44720:CDS |
| CGTAAAGCTCAAAGGGCAGA+AGG | 0.592263 | 7.2:-81173546 | MS.gene44720:CDS |
| TAGACTAGGGAAGGAGGCTG+CGG | 0.594599 | 7.2:-81172891 | MS.gene44720:CDS |
| AGAGGTACCTAGACTAGGGA+AGG | 0.600145 | 7.2:-81172900 | MS.gene44720:CDS |
| TGGTAGAGGTACCTAGACTA+GGG | 0.618791 | 7.2:-81172904 | MS.gene44720:CDS |
| CAACAAAGGTGCTCGTGTGT+TGG | 0.620198 | 7.2:-81172660 | MS.gene44720:CDS |
| TGGCCCCAGTGATACTCACT+TGG | 0.620487 | 7.2:-81172603 | MS.gene44720:CDS |
| ATTGGATGCAAGGCAAGACA+TGG | 0.621464 | 7.2:-81172930 | MS.gene44720:CDS |
| GCAGCCTCCTTCCCTAGTCT+AGG | 0.626818 | 7.2:+81172893 | None:intergenic |
| GGTACCTAGACTAGGGAAGG+AGG | 0.631813 | 7.2:-81172897 | MS.gene44720:CDS |
| TTGCCTTGCATCCAATGAAG+GGG | 0.637154 | 7.2:+81172937 | None:intergenic |
| ACAATTGCTCCTGATAGTGA+AGG | 0.642382 | 7.2:-81172467 | MS.gene44720:CDS |
| GGTACATGATGTACCAACAA+GGG | 0.652620 | 7.2:-81172733 | MS.gene44720:CDS |
| TTGTAATCGGAAATTCCCAA+TGG | 0.669069 | 7.2:+81172335 | None:intergenic |
| AGGTACATGATGTACCAACA+AGG | 0.675679 | 7.2:-81172734 | MS.gene44720:CDS |
| TGCAAGGCAAGACATGGTGG+TGG | 0.676603 | 7.2:-81172924 | MS.gene44720:CDS |
| GATTTGGCTGAGAACAACAA+AGG | 0.678504 | 7.2:-81172674 | MS.gene44720:CDS |
| ATGTTAGCCCAGCTTCACGA+AGG | 0.685337 | 7.2:+81172429 | None:intergenic |
| GTGAAGGCTATAAAAGAATG+GGG | 0.700932 | 7.2:-81172869 | MS.gene44720:CDS |
| CAATGGCCAAGATAGTTGCA+GGG | 0.708011 | 7.2:+81173523 | None:intergenic |
| ACAGGAGAAGGACTTGAATG+GGG | 0.714424 | 7.2:-81172119 | MS.gene44720:CDS |
| GGGGCCACGAAATGTGACAG+CGG | 0.727681 | 7.2:+81172619 | None:intergenic |
| GGATGCAAGGCAAGACATGG+TGG | 0.736388 | 7.2:-81172927 | MS.gene44720:CDS |
| TGTCCAAGTGAGTATCACTG+GGG | 0.825944 | 7.2:+81172600 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTAAATATGATAAATAG+AGG | + | chr7.2:81172247-81172266 | None:intergenic | 10.0% |
| !! | AAGAATACATATATTTGAAA+TGG | + | chr7.2:81172363-81172382 | None:intergenic | 15.0% |
| !!! | TTTGTAACGTATTTTAAATA+TGG | + | chr7.2:81172400-81172419 | None:intergenic | 15.0% |
| !! | AAAACCAATCAAATAATAAC+TGG | + | chr7.2:81172527-81172546 | None:intergenic | 20.0% |
| !!! | AGTTATTATTTGATTGGTTT+TGG | - | chr7.2:81172526-81172545 | MS.gene44720:CDS | 20.0% |
| !!! | GTGATTTTAAAGTAAAAATC+AGG | + | chr7.2:81172163-81172182 | None:intergenic | 20.0% |
| !!! | TAGTGTTTTTTTATGTGAAT+TGG | - | chr7.2:81172314-81172333 | MS.gene44720:CDS | 20.0% |
| !!! | TGATATCAGTAAACAATTTT+AGG | + | chr7.2:81172558-81172577 | None:intergenic | 20.0% |
| ! | AATATAGGTTTCTCAATTTC+TGG | + | chr7.2:81173110-81173129 | None:intergenic | 25.0% |
| ! | ATAATGTGTATCAATTTGGT+AGG | + | chr7.2:81172462-81172481 | None:intergenic | 25.0% |
| ! | CATTTCTTTATAACTACTTC+AGG | - | chr7.2:81172576-81172595 | MS.gene44720:CDS | 25.0% |
| ! | GCAAATAATGTGTATCAATT+TGG | + | chr7.2:81172466-81172485 | None:intergenic | 25.0% |
| !!! | AAGTGAGTAATCTTTGATTT+TGG | + | chr7.2:81172780-81172799 | None:intergenic | 25.0% |
| AAAGAACATCACTAAAGCAT+TGG | - | chr7.2:81173247-81173266 | MS.gene44720:intron | 30.0% | |
| AGAAAGAAATCAACTCAAGA+AGG | - | chr7.2:81173467-81173486 | MS.gene44720:CDS | 30.0% | |
| GAGACCAGTTATTATTTGAT+TGG | - | chr7.2:81172520-81172539 | MS.gene44720:CDS | 30.0% | |
| TCCTAATGTTTGTGAATACA+TGG | - | chr7.2:81172659-81172678 | MS.gene44720:CDS | 30.0% | |
| TGAAATTCGTAAAGCTCAAA+GGG | - | chr7.2:81172066-81172085 | MS.gene44720:CDS | 30.0% | |
| TGAGAAACCTATATTTGAGA+TGG | - | chr7.2:81173115-81173134 | MS.gene44720:intron | 30.0% | |
| ! | AACCTATATTTGAGATGGTT+TGG | - | chr7.2:81173120-81173139 | MS.gene44720:intron | 30.0% |
| ! | ATCATGTACCTTTTCACATA+TGG | + | chr7.2:81172876-81172895 | None:intergenic | 30.0% |
| ! | CCAAAAGATTGAGTTGTAAT+CGG | + | chr7.2:81173300-81173319 | None:intergenic | 30.0% |
| ! | TCATGTACCTTTTCACATAT+GGG | + | chr7.2:81172875-81172894 | None:intergenic | 30.0% |
| ACCTTCTTAAAGATGTTCCT+GGG | - | chr7.2:81173216-81173235 | MS.gene44720:intron | 35.0% | |
| CTGAAATTCGTAAAGCTCAA+AGG | - | chr7.2:81172065-81172084 | MS.gene44720:CDS | 35.0% | |
| CTTTGTTCAACACAATTTGC+AGG | + | chr7.2:81172133-81172152 | None:intergenic | 35.0% | |
| GCCATGTATTCACAAACATT+AGG | + | chr7.2:81172663-81172682 | None:intergenic | 35.0% | |
| GGTGAAGGCTATAAAAGAAT+GGG | - | chr7.2:81172749-81172768 | MS.gene44720:CDS | 35.0% | |
| GTCCAAACCATCTCAAATAT+AGG | + | chr7.2:81173125-81173144 | None:intergenic | 35.0% | |
| GTGAAGGCTATAAAAGAATG+GGG | - | chr7.2:81172750-81172769 | MS.gene44720:CDS | 35.0% | |
| GTGATAAATCCATGATCAAG+AGG | - | chr7.2:81172598-81172617 | MS.gene44720:CDS | 35.0% | |
| TATCAACTCACCAAACTCTT+AGG | - | chr7.2:81172843-81172862 | MS.gene44720:CDS | 35.0% | |
| TGAAATGGAACCTAGAAAAG+TGG | + | chr7.2:81172348-81172367 | None:intergenic | 35.0% | |
| TTGTAATCGGAAATTCCCAA+TGG | + | chr7.2:81173287-81173306 | None:intergenic | 35.0% | |
| ! | CCGATTACAACTCAATCTTT+TGG | - | chr7.2:81173297-81173316 | MS.gene44720:intron | 35.0% |
| ! | CTAGTTGCATTCATCTTTTC+AGG | + | chr7.2:81173383-81173402 | None:intergenic | 35.0% |
| !! | ATGTTCTTTGAAACAATCCC+AGG | + | chr7.2:81173236-81173255 | None:intergenic | 35.0% |
| !! | ATTCATCTTTTCAGGCTTCA+AGG | + | chr7.2:81173375-81173394 | None:intergenic | 35.0% |
| !! | GAGTTTGGTGAGTTGATAAT+CGG | + | chr7.2:81172841-81172860 | None:intergenic | 35.0% |
| !! | GGACTAATTACCACTTTTCT+AGG | - | chr7.2:81172335-81172354 | MS.gene44720:CDS | 35.0% |
| !!! | TGCGTGTGTTTTGTTTATCT+TGG | - | chr7.2:81173436-81173455 | MS.gene44720:CDS | 35.0% |
| AACAGGAGAAGGACTTGAAT+GGG | - | chr7.2:81173499-81173518 | MS.gene44720:CDS | 40.0% | |
| ACCAGGACTTACCATTGAAA+CGG | - | chr7.2:81173541-81173560 | MS.gene44720:CDS | 40.0% | |
| ACCTGCAATTCTAGATCAAG+TGG | - | chr7.2:81173337-81173356 | MS.gene44720:intron | 40.0% | |
| ACGCAAAACAACCGTTTCAA+TGG | + | chr7.2:81173555-81173574 | None:intergenic | 40.0% | |
| AGGTACATGATGTACCAACA+AGG | - | chr7.2:81172885-81172904 | MS.gene44720:CDS | 40.0% | |
| CAAATAGTGCTTGTCCAACA+AGG | + | chr7.2:81173046-81173065 | None:intergenic | 40.0% | |
| CAAGAAGGATTGAAGACAAC+AGG | - | chr7.2:81173482-81173501 | MS.gene44720:CDS | 40.0% | |
| CACCTTCTTAAAGATGTTCC+TGG | - | chr7.2:81173215-81173234 | MS.gene44720:intron | 40.0% | |
| CATGTATCTCCTCTTGATCA+TGG | + | chr7.2:81172610-81172629 | None:intergenic | 40.0% | |
| CGGTGAAGGCTATAAAAGAA+TGG | - | chr7.2:81172748-81172767 | MS.gene44720:CDS | 40.0% | |
| CTTGTTGGACAAGCACTATT+TGG | - | chr7.2:81173044-81173063 | MS.gene44720:intron | 40.0% | |
| GATTTGGCTGAGAACAACAA+AGG | - | chr7.2:81172945-81172964 | MS.gene44720:CDS | 40.0% | |
| GGTACATGATGTACCAACAA+GGG | - | chr7.2:81172886-81172905 | MS.gene44720:CDS | 40.0% | |
| GTTGTTCTCAGCCAAATCTT+TGG | + | chr7.2:81172943-81172962 | None:intergenic | 40.0% | |
| TTAATCGTTTGCACCACAAG+TGG | - | chr7.2:81172798-81172817 | MS.gene44720:CDS | 40.0% | |
| ! | ACAATTGCTCCTGATAGTGA+AGG | - | chr7.2:81173152-81173171 | MS.gene44720:intron | 40.0% |
| ! | ATCTTTTGGATTGCACATCC+CGG | - | chr7.2:81173311-81173330 | MS.gene44720:intron | 40.0% |
| ! | CTTGAATGGGGTGTGTTATT+TGG | - | chr7.2:81173512-81173531 | MS.gene44720:CDS | 40.0% |
| ! | TCCACTTGATCTAGAATTGC+AGG | + | chr7.2:81173341-81173360 | None:intergenic | 40.0% |
| ! | TCCCAGGAACATCTTTAAGA+AGG | + | chr7.2:81173220-81173239 | None:intergenic | 40.0% |
| !! | CATCACTAAAGCATTGGTTG+AGG | - | chr7.2:81173253-81173272 | MS.gene44720:intron | 40.0% |
| !! | GGTTTCTCAATTTCTGGTAC+TGG | + | chr7.2:81173104-81173123 | None:intergenic | 40.0% |
| !!! | GTGTTATTTGGTTTTGGACC+AGG | - | chr7.2:81173524-81173543 | MS.gene44720:CDS | 40.0% |
| !!! | TGGGGTGTGTTATTTGGTTT+TGG | - | chr7.2:81173518-81173537 | MS.gene44720:CDS | 40.0% |
| ACAGGAGAAGGACTTGAATG+GGG | - | chr7.2:81173500-81173519 | MS.gene44720:CDS | 45.0% | |
| ACCGTTTCAATGGTAAGTCC+TGG | + | chr7.2:81173545-81173564 | None:intergenic | 45.0% | |
| ATTGGATGCAAGGCAAGACA+TGG | - | chr7.2:81172689-81172708 | MS.gene44720:CDS | 45.0% | |
| CAACAGGAGAAGGACTTGAA+TGG | - | chr7.2:81173498-81173517 | MS.gene44720:CDS | 45.0% | |
| CAATGGCCAAGATAGTTGCA+GGG | + | chr7.2:81172099-81172118 | None:intergenic | 45.0% | |
| CTGTCCAAGTGAGTATCACT+GGG | + | chr7.2:81173023-81173042 | None:intergenic | 45.0% | |
| CTTGCCTTGCATCCAATGAA+GGG | + | chr7.2:81172686-81172705 | None:intergenic | 45.0% | |
| GATAGTGAAGGAGCCATTGA+TGG | - | chr7.2:81173164-81173183 | MS.gene44720:intron | 45.0% | |
| GGACAAGCACTATTTGGAGA+TGG | - | chr7.2:81173050-81173069 | MS.gene44720:intron | 45.0% | |
| GGATTGAAGACAACAGGAGA+AGG | - | chr7.2:81173488-81173507 | MS.gene44720:CDS | 45.0% | |
| GTCTTCGCCCATATGTGAAA+AGG | - | chr7.2:81172865-81172884 | MS.gene44720:CDS | 45.0% | |
| TCAATGGCTCCTTCACTATC+AGG | + | chr7.2:81173164-81173183 | None:intergenic | 45.0% | |
| TCTTGCCTTGCATCCAATGA+AGG | + | chr7.2:81172687-81172706 | None:intergenic | 45.0% | |
| TGAATACATGGCCCCTTCAT+TGG | - | chr7.2:81172671-81172690 | MS.gene44720:CDS | 45.0% | |
| TGTCCAAGTGAGTATCACTG+GGG | + | chr7.2:81173022-81173041 | None:intergenic | 45.0% | |
| TTCACGAAGGTGACCATCAA+TGG | + | chr7.2:81173180-81173199 | None:intergenic | 45.0% | |
| TTGCCTTGCATCCAATGAAG+GGG | + | chr7.2:81172685-81172704 | None:intergenic | 45.0% | |
| ! | ATCTAGAATTGCAGGTCCAC+CGG | + | chr7.2:81173333-81173352 | None:intergenic | 45.0% |
| !! | TGGTAGAGGTACCTAGACTA+GGG | - | chr7.2:81172715-81172734 | MS.gene44720:CDS | 45.0% |
| !!! | TACCAACAAGGGTGTTTTGC+AGG | - | chr7.2:81172897-81172916 | MS.gene44720:CDS | 45.0% |
| AGAAGGCCCTGCAACTATCT+TGG | - | chr7.2:81172090-81172109 | MS.gene44720:CDS | 50.0% | |
| ATGGTCACCTTCGTGAAGCT+GGG | - | chr7.2:81173183-81173202 | MS.gene44720:intron | 50.0% | |
| ATGTTAGCCCAGCTTCACGA+AGG | + | chr7.2:81173193-81173212 | None:intergenic | 50.0% | |
| CACCTGCAAAACACCCTTGT+TGG | + | chr7.2:81172902-81172921 | None:intergenic | 50.0% | |
| CCAATGGCCAAGATAGTTGC+AGG | + | chr7.2:81172100-81172119 | None:intergenic | 50.0% | |
| CCTGCAACTATCTTGGCCAT+TGG | - | chr7.2:81172097-81172116 | MS.gene44720:CDS | 50.0% | |
| CGTAAAGCTCAAAGGGCAGA+AGG | - | chr7.2:81172073-81172092 | MS.gene44720:CDS | 50.0% | |
| GCTGTCCAAGTGAGTATCAC+TGG | + | chr7.2:81173024-81173043 | None:intergenic | 50.0% | |
| GTTGAGGCTTTCGAGCCATT+GGG | - | chr7.2:81173269-81173288 | MS.gene44720:intron | 50.0% | |
| TGCAGGATTTGCAGTGCCAA+TGG | + | chr7.2:81172116-81172135 | None:intergenic | 50.0% | |
| TGGGCGAAGACCTAAGAGTT+TGG | + | chr7.2:81172856-81172875 | None:intergenic | 50.0% | |
| ! | ACTCACTTGGACAGCCTTGT+TGG | - | chr7.2:81173029-81173048 | MS.gene44720:intron | 50.0% |
| ! | GCTTCGGTTGGCCAAAGATT+TGG | - | chr7.2:81172929-81172948 | MS.gene44720:CDS | 50.0% |
| ! | TCTAGAATTGCAGGTCCACC+GGG | + | chr7.2:81173332-81173351 | None:intergenic | 50.0% |
| ! | TTTTGGATTGCACATCCCGG+TGG | - | chr7.2:81173314-81173333 | MS.gene44720:intron | 50.0% |
| !! | AGAGAAGTGCTCAGCGAGTA+TGG | - | chr7.2:81173401-81173420 | MS.gene44720:intron | 50.0% |
| !! | AGAGGTACCTAGACTAGGGA+AGG | - | chr7.2:81172719-81172738 | MS.gene44720:CDS | 50.0% |
| !! | CAACAAAGGTGCTCGTGTGT+TGG | - | chr7.2:81172959-81172978 | MS.gene44720:CDS | 50.0% |
| !! | GTGAGTTGATAATCGGCTCC+AGG | + | chr7.2:81172834-81172853 | None:intergenic | 50.0% |
| !! | GTGGTAGAGGTACCTAGACT+AGG | - | chr7.2:81172714-81172733 | MS.gene44720:CDS | 50.0% |
| !!! | CAACAAGGGTGTTTTGCAGG+TGG | - | chr7.2:81172900-81172919 | MS.gene44720:CDS | 50.0% |
| AGGCATGTCGACACCACTTG+TGG | + | chr7.2:81172814-81172833 | None:intergenic | 55.0% | |
| GATGGTCACCTTCGTGAAGC+TGG | - | chr7.2:81173182-81173201 | MS.gene44720:intron | 55.0% | |
| GGATGCAAGGCAAGACATGG+TGG | - | chr7.2:81172692-81172711 | MS.gene44720:CDS | 55.0% | |
| GGTACCTAGACTAGGGAAGG+AGG | - | chr7.2:81172722-81172741 | MS.gene44720:CDS | 55.0% | |
| GGTTGAGGCTTTCGAGCCAT+TGG | - | chr7.2:81173268-81173287 | MS.gene44720:intron | 55.0% | |
| GTCACCGCTGTCACATTTCG+TGG | - | chr7.2:81172996-81173015 | MS.gene44720:CDS | 55.0% | |
| TAGACTAGGGAAGGAGGCTG+CGG | - | chr7.2:81172728-81172747 | MS.gene44720:CDS | 55.0% | |
| TGCAAGGCAAGACATGGTGG+TGG | - | chr7.2:81172695-81172714 | MS.gene44720:CDS | 55.0% | |
| TGGCCCCAGTGATACTCACT+TGG | - | chr7.2:81173016-81173035 | MS.gene44720:CDS | 55.0% | |
| TGGCCCCTTCATTGGATGCA+AGG | - | chr7.2:81172679-81172698 | MS.gene44720:CDS | 55.0% | |
| !! | ACAAGTGGTGTCGACATGCC+TGG | - | chr7.2:81172813-81172832 | MS.gene44720:CDS | 55.0% |
| !! | AGGGTGTTTTGCAGGTGGCA+CGG | - | chr7.2:81172905-81172924 | MS.gene44720:CDS | 55.0% |
| !! | GCAAGACATGGTGGTGGTAG+AGG | - | chr7.2:81172701-81172720 | MS.gene44720:CDS | 55.0% |
| GCAGCCTCCTTCCCTAGTCT+AGG | + | chr7.2:81172729-81172748 | None:intergenic | 60.0% | |
| GGAGCTGCTGCACTCGTTGT+TGG | - | chr7.2:81173071-81173090 | MS.gene44720:intron | 60.0% | |
| GGGGCCACGAAATGTGACAG+CGG | + | chr7.2:81173003-81173022 | None:intergenic | 60.0% | |
| TTGCAGGTGGCACGGTGCTT+CGG | - | chr7.2:81172913-81172932 | MS.gene44720:CDS | 60.0% | |
| AGGGAAGGAGGCTGCGGTGA+AGG | - | chr7.2:81172734-81172753 | MS.gene44720:CDS | 65.0% | |
| AGGTGGCACGGTGCTTCGGT+TGG | - | chr7.2:81172917-81172936 | MS.gene44720:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 81172052 | 81173589 | 81172052 | ID=MS.gene44720 |
| chr7.2 | mRNA | 81172052 | 81173589 | 81172052 | ID=MS.gene44720.t1;Parent=MS.gene44720 |
| chr7.2 | exon | 81173412 | 81173589 | 81173412 | ID=MS.gene44720.t1.exon1;Parent=MS.gene44720.t1 |
| chr7.2 | CDS | 81173412 | 81173589 | 81173412 | ID=cds.MS.gene44720.t1;Parent=MS.gene44720.t1 |
| chr7.2 | exon | 81172052 | 81173043 | 81172052 | ID=MS.gene44720.t1.exon2;Parent=MS.gene44720.t1 |
| chr7.2 | CDS | 81172052 | 81173043 | 81172052 | ID=cds.MS.gene44720.t1;Parent=MS.gene44720.t1 |
| Gene Sequence |
| Protein sequence |
