Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44723.t1 | P30074.1 | 99.2 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 7.30E-221 | 776.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44723.t1 | P30074 | 99.2 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 1.8e-223 | 776.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44723.t1 | G7KR33 | 99.2 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 6.9e-221 | 775.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene44723 | 0.800843 | 1.19E-48 | -1.69E-46 |
| MS.gene049232 | MS.gene44723 | -0.80133 | 9.47E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene44723 | 0.840656 | 7.62E-58 | -1.69E-46 |
| MS.gene050381 | MS.gene44723 | 0.855921 | 4.56E-62 | -1.69E-46 |
| MS.gene050883 | MS.gene44723 | 0.917508 | 4.87E-86 | -1.69E-46 |
| MS.gene051127 | MS.gene44723 | 0.851733 | 7.32E-61 | -1.69E-46 |
| MS.gene05141 | MS.gene44723 | 0.817723 | 2.84E-52 | -1.69E-46 |
| MS.gene05142 | MS.gene44723 | 0.811977 | 5.35E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene44723 | 0.867023 | 1.86E-65 | -1.69E-46 |
| MS.gene052053 | MS.gene44723 | 0.826291 | 2.94E-54 | -1.69E-46 |
| MS.gene052089 | MS.gene44723 | 0.807744 | 4.35E-50 | -1.69E-46 |
| MS.gene052482 | MS.gene44723 | -0.80677 | 7.00E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene44723 | -0.806245 | 9.03E-50 | -1.69E-46 |
| MS.gene053069 | MS.gene44723 | 0.814356 | 1.61E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene44723 | 0.806263 | 8.95E-50 | -1.69E-46 |
| MS.gene053549 | MS.gene44723 | 0.843958 | 1.02E-58 | -1.69E-46 |
| MS.gene053561 | MS.gene44723 | 0.804634 | 1.97E-49 | -1.69E-46 |
| MS.gene053826 | MS.gene44723 | 0.86385 | 1.85E-64 | -1.69E-46 |
| MS.gene054708 | MS.gene44723 | 0.822777 | 1.98E-53 | -1.69E-46 |
| MS.gene055124 | MS.gene44723 | 0.809962 | 1.46E-50 | -1.69E-46 |
| MS.gene055139 | MS.gene44723 | 0.807191 | 5.70E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene44723 | 0.815066 | 1.12E-51 | -1.69E-46 |
| MS.gene055401 | MS.gene44723 | 0.815623 | 8.41E-52 | -1.69E-46 |
| MS.gene055549 | MS.gene44723 | 0.830567 | 2.73E-55 | -1.69E-46 |
| MS.gene055550 | MS.gene44723 | 0.828505 | 8.66E-55 | -1.69E-46 |
| MS.gene055551 | MS.gene44723 | 0.842456 | 2.56E-58 | -1.69E-46 |
| MS.gene056310 | MS.gene44723 | 0.90949 | 5.38E-82 | -1.69E-46 |
| MS.gene056311 | MS.gene44723 | 0.931299 | 4.63E-94 | -1.69E-46 |
| MS.gene056313 | MS.gene44723 | 0.869098 | 3.99E-66 | -1.69E-46 |
| MS.gene056408 | MS.gene44723 | 0.828391 | 9.23E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene44723 | -0.803783 | 2.96E-49 | -1.69E-46 |
| MS.gene056530 | MS.gene44723 | 0.864139 | 1.51E-64 | -1.69E-46 |
| MS.gene057014 | MS.gene44723 | 0.86528 | 6.62E-65 | -1.69E-46 |
| MS.gene057499 | MS.gene44723 | 0.810145 | 1.33E-50 | -1.69E-46 |
| MS.gene058109 | MS.gene44723 | 0.847161 | 1.38E-59 | -1.69E-46 |
| MS.gene058111 | MS.gene44723 | 0.856901 | 2.35E-62 | -1.69E-46 |
| MS.gene058171 | MS.gene44723 | 0.846507 | 2.08E-59 | -1.69E-46 |
| MS.gene058173 | MS.gene44723 | 0.848641 | 5.39E-60 | -1.69E-46 |
| MS.gene058175 | MS.gene44723 | 0.847894 | 8.66E-60 | -1.69E-46 |
| MS.gene058493 | MS.gene44723 | 0.865417 | 6.00E-65 | -1.69E-46 |
| MS.gene058494 | MS.gene44723 | 0.85985 | 3.11E-63 | -1.69E-46 |
| MS.gene058496 | MS.gene44723 | 0.80781 | 4.21E-50 | -1.69E-46 |
| MS.gene058628 | MS.gene44723 | 0.851999 | 6.16E-61 | -1.69E-46 |
| MS.gene058629 | MS.gene44723 | 0.834786 | 2.45E-56 | -1.69E-46 |
| MS.gene058630 | MS.gene44723 | 0.855138 | 7.71E-62 | -1.69E-46 |
| MS.gene05884 | MS.gene44723 | 0.835749 | 1.40E-56 | -1.69E-46 |
| MS.gene059052 | MS.gene44723 | 0.806589 | 7.64E-50 | -1.69E-46 |
| MS.gene059223 | MS.gene44723 | 0.819669 | 1.03E-52 | -1.69E-46 |
| MS.gene059236 | MS.gene44723 | 0.805196 | 1.50E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene44723 | -0.805702 | 1.18E-49 | -1.69E-46 |
| MS.gene060187 | MS.gene44723 | 0.810275 | 1.25E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene44723 | 0.824793 | 6.66E-54 | -1.69E-46 |
| MS.gene060778 | MS.gene44723 | -0.801366 | 9.31E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene44723 | -0.821836 | 3.27E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44723.t1 | MTR_7g016820 | 99.229 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 0.0 | 800 |
| MS.gene44723.t1 | MTR_7g016720 | 99.229 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 0.0 | 800 |
| MS.gene44723.t1 | MTR_7g016780 | 98.715 | 389 | 5 | 0 | 1 | 389 | 1 | 389 | 0.0 | 797 |
| MS.gene44723.t1 | MTR_7g016800 | 98.458 | 389 | 6 | 0 | 1 | 389 | 1 | 389 | 0.0 | 796 |
| MS.gene44723.t1 | MTR_7g016700 | 98.458 | 389 | 6 | 0 | 1 | 389 | 1 | 389 | 0.0 | 796 |
| MS.gene44723.t1 | MTR_1g098140 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 793 |
| MS.gene44723.t1 | MTR_5g007730 | 97.943 | 389 | 8 | 0 | 1 | 389 | 1 | 389 | 0.0 | 793 |
| MS.gene44723.t1 | MTR_1g097935 | 97.943 | 389 | 8 | 0 | 1 | 389 | 1 | 389 | 0.0 | 790 |
| MS.gene44723.t1 | MTR_1g097910 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 789 |
| MS.gene44723.t1 | MTR_5g007760 | 96.658 | 389 | 13 | 0 | 1 | 389 | 1 | 389 | 0.0 | 782 |
| MS.gene44723.t1 | MTR_5g007717 | 95.887 | 389 | 16 | 0 | 1 | 389 | 1 | 389 | 0.0 | 778 |
| MS.gene44723.t1 | MTR_5g007713 | 95.887 | 389 | 16 | 0 | 1 | 389 | 1 | 389 | 0.0 | 776 |
| MS.gene44723.t1 | MTR_3g083920 | 90.722 | 388 | 36 | 0 | 1 | 388 | 1 | 388 | 0.0 | 748 |
| MS.gene44723.t1 | MTR_3g083910 | 89.948 | 388 | 39 | 0 | 1 | 388 | 1 | 388 | 0.0 | 745 |
| MS.gene44723.t1 | MTR_2g058470 | 87.404 | 389 | 49 | 0 | 1 | 389 | 1 | 389 | 0.0 | 716 |
| MS.gene44723.t1 | MTR_7g084300 | 80.977 | 389 | 74 | 0 | 1 | 389 | 1 | 389 | 0.0 | 677 |
| MS.gene44723.t1 | MTR_3g086260 | 81.701 | 388 | 71 | 0 | 1 | 388 | 1 | 388 | 0.0 | 677 |
| MS.gene44723.t1 | MTR_7g113405 | 96.040 | 303 | 12 | 0 | 87 | 389 | 1 | 303 | 0.0 | 597 |
| MS.gene44723.t1 | MTR_3g088675 | 66.838 | 389 | 129 | 0 | 1 | 389 | 1 | 389 | 0.0 | 580 |
| MS.gene44723.t1 | MTR_5g007740 | 80.368 | 326 | 64 | 0 | 64 | 389 | 1 | 326 | 0.0 | 549 |
| MS.gene44723.t1 | MTR_5g007723 | 68.262 | 397 | 77 | 5 | 12 | 389 | 32 | 398 | 0.0 | 528 |
| MS.gene44723.t1 | MTR_5g007770 | 77.333 | 300 | 35 | 2 | 70 | 369 | 1 | 267 | 8.17e-167 | 468 |
| MS.gene44723.t1 | MTR_3g088670 | 48.974 | 390 | 185 | 6 | 10 | 388 | 2 | 388 | 1.89e-130 | 380 |
| MS.gene44723.t1 | MTR_4g075560 | 43.158 | 380 | 209 | 4 | 11 | 387 | 13 | 388 | 2.81e-104 | 313 |
| MS.gene44723.t1 | MTR_4g078730 | 37.802 | 373 | 225 | 2 | 15 | 387 | 24 | 389 | 5.87e-93 | 284 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44723.t1 | AT5G13930 | 82.732 | 388 | 66 | 1 | 3 | 389 | 8 | 395 | 0.0 | 677 |
| MS.gene44723.t1 | AT4G34850 | 41.114 | 377 | 218 | 3 | 15 | 389 | 18 | 392 | 3.67e-98 | 298 |
| MS.gene44723.t1 | AT4G00040 | 40.051 | 392 | 223 | 6 | 1 | 387 | 1 | 385 | 1.62e-96 | 293 |
| MS.gene44723.t1 | AT1G02050 | 39.332 | 389 | 225 | 6 | 2 | 387 | 12 | 392 | 6.38e-88 | 271 |
| MS.gene44723.t1 | AT4G34850 | 40.625 | 320 | 187 | 2 | 72 | 389 | 1 | 319 | 1.24e-82 | 255 |
Find 94 sgRNAs with CRISPR-Local
Find 137 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATAGGTTTCTCAATTTC+TGG | 0.117134 | 7.2:+81078887 | None:intergenic |
| CTTGAATGGGGTGTATTATT+TGG | 0.169989 | 7.2:-81078482 | MS.gene44723:CDS |
| TCCTAATGTTTGTGAATATA+TGG | 0.250140 | 7.2:-81079335 | MS.gene44723:CDS |
| AGAGAAGTGCTTAGTGAATA+TGG | 0.285983 | 7.2:-81078593 | MS.gene44723:CDS |
| TGGGGTGTATTATTTGGTTT+TGG | 0.289261 | 7.2:-81078476 | MS.gene44723:CDS |
| CCGATTACAACTCAATCTTT+TGG | 0.295351 | 7.2:-81078697 | MS.gene44723:CDS |
| AACCTATATTTGAGATGGTT+TGG | 0.308691 | 7.2:-81078874 | MS.gene44723:CDS |
| CTTGTTGGACAAGCACTATT+TGG | 0.314421 | 7.2:-81078950 | MS.gene44723:CDS |
| TCCACTTGATCTAGAATTGC+AGG | 0.315800 | 7.2:+81078656 | None:intergenic |
| TATCAACTCACCAAACTCTT+AGG | 0.335572 | 7.2:-81079151 | MS.gene44723:CDS |
| GCTTCGTTTGGCTAAAGATT+TGG | 0.342769 | 7.2:-81079065 | MS.gene44723:CDS |
| CTTTGTTCAACACAATTTGC+AGG | 0.350727 | 7.2:+81080057 | None:intergenic |
| GGTTTCTCAATTTCTGGTGC+TGG | 0.353666 | 7.2:+81078893 | None:intergenic |
| TCAATGGCTCCTTCACTATC+AGG | 0.368167 | 7.2:+81078833 | None:intergenic |
| AAGTGAGTAATCTTTGACTT+TGG | 0.381814 | 7.2:+81079217 | None:intergenic |
| CACCTTCTTAAAGATGTTCC+TGG | 0.385004 | 7.2:-81078779 | MS.gene44723:CDS |
| TGAATATATGGCACCTTCCT+TGG | 0.393161 | 7.2:-81079323 | MS.gene44723:CDS |
| GCCATATATTCACAAACATT+AGG | 0.398680 | 7.2:+81079334 | None:intergenic |
| GAGTTTGGTGAGTTGATAAT+CGG | 0.404976 | 7.2:+81079156 | None:intergenic |
| AGGTGGCACGGTGCTTCGTT+TGG | 0.407834 | 7.2:-81079077 | MS.gene44723:CDS |
| CCAAAAGATTGAGTTGTAAT+CGG | 0.409783 | 7.2:+81078697 | None:intergenic |
| GGAGCTGCTGCACTCATTGT+TGG | 0.411529 | 7.2:-81078923 | MS.gene44723:CDS |
| CCAATGGCCAAGATAGTTGC+AGG | 0.424125 | 7.2:+81080090 | None:intergenic |
| CACCTGCAAAACACCCTTGT+TGG | 0.425276 | 7.2:+81079095 | None:intergenic |
| ACTCACTTGGACAGCCTTGT+TGG | 0.426712 | 7.2:-81078965 | MS.gene44723:CDS |
| GTCCAAACCATCTCAAATAT+AGG | 0.437103 | 7.2:+81078872 | None:intergenic |
| GGTCGAGGCTTTCGAGCCAT+TGG | 0.443755 | 7.2:-81078726 | MS.gene44723:CDS |
| AACGGGAGAAGGACTTGAAT+GGG | 0.444037 | 7.2:-81078495 | MS.gene44723:CDS |
| GTCACCGCTGTCACATTTCG+TGG | 0.448537 | 7.2:-81078998 | MS.gene44723:CDS |
| TGGGCGAAGACCTAAGAGTT+TGG | 0.450749 | 7.2:+81079141 | None:intergenic |
| AGAAGGTCCTGCAACTATCT+TGG | 0.450898 | 7.2:-81080097 | MS.gene44723:CDS |
| GCGCAAAACAACCGTTTCAA+TGG | 0.451768 | 7.2:+81078442 | None:intergenic |
| ATCTAGAATTGCAGGTCCAC+CGG | 0.463612 | 7.2:+81078664 | None:intergenic |
| AGTGAAGGCTATAAAAGAAT+GGG | 0.468743 | 7.2:-81079245 | MS.gene44723:CDS |
| CATGTATCTCCTCTTGATCA+TGG | 0.469562 | 7.2:+81079387 | None:intergenic |
| GTCGAGGCTTTCGAGCCATT+GGG | 0.470601 | 7.2:-81078725 | MS.gene44723:CDS |
| TTCACGAAGGTGACCATCAA+TGG | 0.471758 | 7.2:+81078817 | None:intergenic |
| TGGCACCTTCCTTGGATGCT+AGG | 0.472685 | 7.2:-81079315 | MS.gene44723:CDS |
| GCAAGACATGGTGGTGGTAG+AGG | 0.474656 | 7.2:-81079293 | MS.gene44723:CDS |
| AAAGAACATTACTAAAGCAT+TGG | 0.475145 | 7.2:-81078747 | MS.gene44723:CDS |
| ACCTTCTTAAAGATGTTCCT+GGG | 0.484500 | 7.2:-81078778 | MS.gene44723:CDS |
| GATGGTCACCTTCGTGAAGC+TGG | 0.486168 | 7.2:-81078812 | MS.gene44723:CDS |
| GATAGTGAAGGAGCCATTGA+TGG | 0.488582 | 7.2:-81078830 | MS.gene44723:CDS |
| CAGTGAAGGCTATAAAAGAA+TGG | 0.502364 | 7.2:-81079246 | MS.gene44723:CDS |
| CAACGGGAGAAGGACTTGAA+TGG | 0.504753 | 7.2:-81078496 | MS.gene44723:CDS |
| GTCTTCGCCCATATGTGAAA+AGG | 0.504788 | 7.2:-81079129 | MS.gene44723:CDS |
| TGAGAAACCTATATTTGAGA+TGG | 0.507221 | 7.2:-81078879 | MS.gene44723:CDS |
| AAAGACGGATTGAAGACAAC+GGG | 0.508808 | 7.2:-81078512 | MS.gene44723:CDS |
| ACCAGGACTTACCATTGAAA+CGG | 0.510913 | 7.2:-81078453 | MS.gene44723:CDS |
| GTGAGTTGATAATCGGCTCC+AGG | 0.515720 | 7.2:+81079163 | None:intergenic |
| TGCAGGATTTGCAGTGCCAA+TGG | 0.515817 | 7.2:+81080074 | None:intergenic |
| CATGTCTTGCCTAGCATCCA+AGG | 0.526806 | 7.2:+81079306 | None:intergenic |
| ACCGTTTCAATGGTAAGTCC+TGG | 0.536054 | 7.2:+81078452 | None:intergenic |
| AGGGAAGGAGGCTGCAGTGA+AGG | 0.537534 | 7.2:-81079260 | MS.gene44723:CDS |
| TCCCAGGAACATCTTTAAGA+AGG | 0.550142 | 7.2:+81078777 | None:intergenic |
| ATGTTCTTTGAAACAATCCC+AGG | 0.550779 | 7.2:+81078761 | None:intergenic |
| CTGTCCAAGTGAGTATCACT+AGG | 0.551596 | 7.2:+81078974 | None:intergenic |
| GTGGTAGAGGTACCTAGACT+AGG | 0.557229 | 7.2:-81079280 | MS.gene44723:CDS |
| GGACAAGCACTATTTGGAGA+TGG | 0.563405 | 7.2:-81078944 | MS.gene44723:CDS |
| ACCTGCAATTCTAGATCAAG+TGG | 0.566779 | 7.2:-81078657 | MS.gene44723:CDS |
| AGGCATGTCTACACCACTTG+TGG | 0.569916 | 7.2:+81079183 | None:intergenic |
| AGAAAGAAATCAACTAAAGA+CGG | 0.570835 | 7.2:-81078527 | MS.gene44723:CDS |
| TCTAGAATTGCAGGTCCACC+GGG | 0.570884 | 7.2:+81078665 | None:intergenic |
| ACAAGTGGTGTAGACATGCC+TGG | 0.572074 | 7.2:-81079181 | MS.gene44723:CDS |
| CAAATAGTGCTTGTCCAACA+AGG | 0.572762 | 7.2:+81078951 | None:intergenic |
| GCAGCCTCCTTCCCTAGTCT+AGG | 0.573798 | 7.2:+81079268 | None:intergenic |
| CCTGCAACTATCTTGGCCAT+TGG | 0.574132 | 7.2:-81080090 | MS.gene44723:CDS |
| GGATTGAAGACAACGGGAGA+AGG | 0.579099 | 7.2:-81078506 | MS.gene44723:CDS |
| GTGATAAATCCATGATCAAG+AGG | 0.584564 | 7.2:-81079396 | MS.gene44723:CDS |
| TTAATCGTTTGCACCACAAG+TGG | 0.591579 | 7.2:-81079196 | MS.gene44723:CDS |
| TCACCGCTGTCACATTTCGT+GGG | 0.591938 | 7.2:-81078997 | MS.gene44723:CDS |
| CATTACTAAAGCATTGGTCG+AGG | 0.596202 | 7.2:-81078741 | MS.gene44723:CDS |
| TGTGTTGGTTGTTTGTTCTG+AGG | 0.596318 | 7.2:-81079020 | MS.gene44723:CDS |
| CGTAAAGCTCAAAGAGCAGA+AGG | 0.599601 | 7.2:-81080114 | MS.gene44723:CDS |
| AGAGGTACCTAGACTAGGGA+AGG | 0.600145 | 7.2:-81079275 | MS.gene44723:CDS |
| CTTGGATGCTAGGCAAGACA+TGG | 0.600480 | 7.2:-81079305 | MS.gene44723:CDS |
| TGGTAGAGGTACCTAGACTA+GGG | 0.618791 | 7.2:-81079279 | MS.gene44723:CDS |
| CAACAAAGGTGCTCGTGTGT+TGG | 0.620198 | 7.2:-81079035 | MS.gene44723:CDS |
| TGGGCCTAGTGATACTCACT+TGG | 0.622076 | 7.2:-81078978 | MS.gene44723:CDS |
| GAGGAGATACATGTATCTAA+CGG | 0.627847 | 7.2:-81079377 | MS.gene44723:CDS |
| GGTACCTAGACTAGGGAAGG+AGG | 0.631813 | 7.2:-81079272 | MS.gene44723:CDS |
| TCTTGCCTAGCATCCAAGGA+AGG | 0.637347 | 7.2:+81079310 | None:intergenic |
| ACAATTGCTCCTGATAGTGA+AGG | 0.642382 | 7.2:-81078842 | MS.gene44723:CDS |
| GGTACATGATGTACCAACAA+GGG | 0.652620 | 7.2:-81079108 | MS.gene44723:CDS |
| TTGTAATCGGAAATTCCCAA+TGG | 0.669069 | 7.2:+81078710 | None:intergenic |
| TGCTAGGCAAGACATGGTGG+TGG | 0.675229 | 7.2:-81079299 | MS.gene44723:CDS |
| AGGTACATGATGTACCAACA+AGG | 0.675679 | 7.2:-81079109 | MS.gene44723:CDS |
| TAAAGACGGATTGAAGACAA+CGG | 0.676404 | 7.2:-81078513 | MS.gene44723:CDS |
| GTGAAGGCTATAAAAGAATG+GGG | 0.681481 | 7.2:-81079244 | MS.gene44723:CDS |
| GATTTGGCTGAGAACAACAA+AGG | 0.683242 | 7.2:-81079049 | MS.gene44723:CDS |
| ATGTTAGTCCAGCTTCACGA+AGG | 0.698828 | 7.2:+81078804 | None:intergenic |
| GGATGCTAGGCAAGACATGG+TGG | 0.713925 | 7.2:-81079302 | MS.gene44723:CDS |
| ACGGGAGAAGGACTTGAATG+GGG | 0.714424 | 7.2:-81078494 | MS.gene44723:CDS |
| AGGCCCACGAAATGTGACAG+CGG | 0.766137 | 7.2:+81078994 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACAATCAAATAATAAT+TGG | + | chr7.2:81079102-81079121 | None:intergenic | 10.0% |
| !! | AAGAATAAATATATTTGAAA+TGG | + | chr7.2:81078931-81078950 | None:intergenic | 10.0% |
| !!! | AATTATTATTTGATTGTTTT+TGG | - | chr7.2:81079101-81079120 | MS.gene44723:CDS | 10.0% |
| !!! | TATTTAACTTACTTAAAATA+CGG | - | chr7.2:81078966-81078985 | MS.gene44723:CDS | 10.0% |
| !!! | TATTTTAAGTAAGTTAAATA+TGG | + | chr7.2:81078966-81078985 | None:intergenic | 10.0% |
| !! | GAGAGAATATAATTATTTAA+TGG | + | chr7.2:81078719-81078738 | None:intergenic | 15.0% |
| !! | TTCCTTAAAAAATAATATAG+AGG | + | chr7.2:81078622-81078641 | None:intergenic | 15.0% |
| !!! | TACCTCTATATTATTTTTTA+AGG | - | chr7.2:81078617-81078636 | MS.gene44723:CDS | 15.0% |
| !!! | TTTTTTTTCATTTTGTGAAT+TGG | - | chr7.2:81078883-81078902 | MS.gene44723:CDS | 15.0% |
| !! | ACAAATAATGTGTTTCAATT+TGG | + | chr7.2:81079041-81079060 | None:intergenic | 20.0% |
| !!! | GTGATTTTAAAGTAAAAATC+AGG | + | chr7.2:81078538-81078557 | None:intergenic | 20.0% |
| !!! | TTGAAATTTTTCTTTGAGTT+CGG | + | chr7.2:81078578-81078597 | None:intergenic | 20.0% |
| ! | AAAGAACATTACTAAAGCAT+TGG | - | chr7.2:81079815-81079834 | MS.gene44723:intron | 25.0% |
| ! | AATATAGGTTTCTCAATTTC+TGG | + | chr7.2:81079678-81079697 | None:intergenic | 25.0% |
| ! | AATGGAAATAAAAACATCCA+AGG | + | chr7.2:81078701-81078720 | None:intergenic | 25.0% |
| ! | AGAAAGAAATCAACTAAAGA+CGG | - | chr7.2:81080035-81080054 | MS.gene44723:CDS | 25.0% |
| ! | ATAATGTGTTTCAATTTGGT+AGG | + | chr7.2:81079037-81079056 | None:intergenic | 25.0% |
| ! | ATGGAAATAAAAACATCCAA+GGG | + | chr7.2:81078700-81078719 | None:intergenic | 25.0% |
| ! | TCATTTCTTATAACTACTTC+AGG | - | chr7.2:81079144-81079163 | MS.gene44723:CDS | 25.0% |
| ! | TCCTAATGTTTGTGAATATA+TGG | - | chr7.2:81079227-81079246 | MS.gene44723:CDS | 25.0% |
| ! | TGGACTAATTAACAATTTCT+AGG | - | chr7.2:81078903-81078922 | MS.gene44723:CDS | 25.0% |
| !! | TAAGTTAAATATGGTGATGA+TGG | + | chr7.2:81078957-81078976 | None:intergenic | 25.0% |
| AGTGAAGGCTATAAAAGAAT+GGG | - | chr7.2:81079317-81079336 | MS.gene44723:CDS | 30.0% | |
| GCCATATATTCACAAACATT+AGG | + | chr7.2:81079231-81079250 | None:intergenic | 30.0% | |
| GGCAATATTAATATCATGAG+TGG | + | chr7.2:81079016-81079035 | None:intergenic | 30.0% | |
| TGAGAAACCTATATTTGAGA+TGG | - | chr7.2:81079683-81079702 | MS.gene44723:intron | 30.0% | |
| ! | AACCTATATTTGAGATGGTT+TGG | - | chr7.2:81079688-81079707 | MS.gene44723:intron | 30.0% |
| ! | AAGTGAGTAATCTTTGACTT+TGG | + | chr7.2:81079348-81079367 | None:intergenic | 30.0% |
| ! | ATCATGTACCTTTTCACATA+TGG | + | chr7.2:81079444-81079463 | None:intergenic | 30.0% |
| ! | CCAAAAGATTGAGTTGTAAT+CGG | + | chr7.2:81079868-81079887 | None:intergenic | 30.0% |
| ! | TCATGTACCTTTTCACATAT+GGG | + | chr7.2:81079443-81079462 | None:intergenic | 30.0% |
| !! | ATTCATCTTTTCAGGCTTTA+AGG | + | chr7.2:81079943-81079962 | None:intergenic | 30.0% |
| ACCTTCTTAAAGATGTTCCT+GGG | - | chr7.2:81079784-81079803 | MS.gene44723:intron | 35.0% | |
| CAGTGAAGGCTATAAAAGAA+TGG | - | chr7.2:81079316-81079335 | MS.gene44723:CDS | 35.0% | |
| CTTGAATGGGGTGTATTATT+TGG | - | chr7.2:81080080-81080099 | MS.gene44723:CDS | 35.0% | |
| CTTTGTTCAACACAATTTGC+AGG | + | chr7.2:81078508-81078527 | None:intergenic | 35.0% | |
| GAAATAAAAACATCCAAGGG+TGG | + | chr7.2:81078697-81078716 | None:intergenic | 35.0% | |
| GAGGAGATACATGTATCTAA+CGG | - | chr7.2:81079185-81079204 | MS.gene44723:CDS | 35.0% | |
| GTCCAAACCATCTCAAATAT+AGG | + | chr7.2:81079693-81079712 | None:intergenic | 35.0% | |
| GTGAAGGCTATAAAAGAATG+GGG | - | chr7.2:81079318-81079337 | MS.gene44723:CDS | 35.0% | |
| GTGATAAATCCATGATCAAG+AGG | - | chr7.2:81079166-81079185 | MS.gene44723:CDS | 35.0% | |
| TATCAACTCACCAAACTCTT+AGG | - | chr7.2:81079411-81079430 | MS.gene44723:intron | 35.0% | |
| TTGTAATCGGAAATTCCCAA+TGG | + | chr7.2:81079855-81079874 | None:intergenic | 35.0% | |
| ! | CCGATTACAACTCAATCTTT+TGG | - | chr7.2:81079865-81079884 | MS.gene44723:intron | 35.0% |
| ! | CTAGTTGCATTCATCTTTTC+AGG | + | chr7.2:81079951-81079970 | None:intergenic | 35.0% |
| !! | AGAGAAGTGCTTAGTGAATA+TGG | - | chr7.2:81079969-81079988 | MS.gene44723:intron | 35.0% |
| !! | ATGTTCTTTGAAACAATCCC+AGG | + | chr7.2:81079804-81079823 | None:intergenic | 35.0% |
| !! | GAGTTTGGTGAGTTGATAAT+CGG | + | chr7.2:81079409-81079428 | None:intergenic | 35.0% |
| !! | TAAAGACGGATTGAAGACAA+CGG | - | chr7.2:81080049-81080068 | MS.gene44723:CDS | 35.0% |
| !! | TGGGGTGTATTATTTGGTTT+TGG | - | chr7.2:81080086-81080105 | MS.gene44723:CDS | 35.0% |
| !!! | GTATTATTTGGTTTTGGACC+AGG | - | chr7.2:81080092-81080111 | MS.gene44723:CDS | 35.0% |
| ACCAGGACTTACCATTGAAA+CGG | - | chr7.2:81080109-81080128 | MS.gene44723:CDS | 40.0% | |
| ACCTGCAATTCTAGATCAAG+TGG | - | chr7.2:81079905-81079924 | MS.gene44723:intron | 40.0% | |
| AGGTACATGATGTACCAACA+AGG | - | chr7.2:81079453-81079472 | MS.gene44723:intron | 40.0% | |
| CAAATAGTGCTTGTCCAACA+AGG | + | chr7.2:81079614-81079633 | None:intergenic | 40.0% | |
| CACCTTCTTAAAGATGTTCC+TGG | - | chr7.2:81079783-81079802 | MS.gene44723:intron | 40.0% | |
| CATGTATCTCCTCTTGATCA+TGG | + | chr7.2:81079178-81079197 | None:intergenic | 40.0% | |
| CTTGTTGGACAAGCACTATT+TGG | - | chr7.2:81079612-81079631 | MS.gene44723:intron | 40.0% | |
| GATTTGGCTGAGAACAACAA+AGG | - | chr7.2:81079513-81079532 | MS.gene44723:intron | 40.0% | |
| GCTTCGTTTGGCTAAAGATT+TGG | - | chr7.2:81079497-81079516 | MS.gene44723:intron | 40.0% | |
| GGTACATGATGTACCAACAA+GGG | - | chr7.2:81079454-81079473 | MS.gene44723:intron | 40.0% | |
| TGAATATATGGCACCTTCCT+TGG | - | chr7.2:81079239-81079258 | MS.gene44723:CDS | 40.0% | |
| TTAATCGTTTGCACCACAAG+TGG | - | chr7.2:81079366-81079385 | MS.gene44723:CDS | 40.0% | |
| ! | ACAATTGCTCCTGATAGTGA+AGG | - | chr7.2:81079720-81079739 | MS.gene44723:intron | 40.0% |
| ! | ATCTTTTGGATTGCACATCC+CGG | - | chr7.2:81079879-81079898 | MS.gene44723:intron | 40.0% |
| ! | TCCACTTGATCTAGAATTGC+AGG | + | chr7.2:81079909-81079928 | None:intergenic | 40.0% |
| ! | TCCCAGGAACATCTTTAAGA+AGG | + | chr7.2:81079788-81079807 | None:intergenic | 40.0% |
| !! | AAAGACGGATTGAAGACAAC+GGG | - | chr7.2:81080050-81080069 | MS.gene44723:CDS | 40.0% |
| !! | CATTACTAAAGCATTGGTCG+AGG | - | chr7.2:81079821-81079840 | MS.gene44723:intron | 40.0% |
| !! | TGTGTTGGTTGTTTGTTCTG+AGG | - | chr7.2:81079542-81079561 | MS.gene44723:intron | 40.0% |
| AACGGGAGAAGGACTTGAAT+GGG | - | chr7.2:81080067-81080086 | MS.gene44723:CDS | 45.0% | |
| AAGCACACAAGCGGAATTTG+TGG | + | chr7.2:81078773-81078792 | None:intergenic | 45.0% | |
| AAGCGGTGGAAAGGAAAACA+TGG | + | chr7.2:81078745-81078764 | None:intergenic | 45.0% | |
| AAGGGTGGTAATAGCTTCGT+GGG | + | chr7.2:81078682-81078701 | None:intergenic | 45.0% | |
| AATTTGTGGACATCAAGCGG+TGG | + | chr7.2:81078759-81078778 | None:intergenic | 45.0% | |
| ACCGTTTCAATGGTAAGTCC+TGG | + | chr7.2:81080113-81080132 | None:intergenic | 45.0% | |
| AGAAGGTCCTGCAACTATCT+TGG | - | chr7.2:81078465-81078484 | MS.gene44723:CDS | 45.0% | |
| AGCGGTGGAAAGGAAAACAT+GGG | + | chr7.2:81078744-81078763 | None:intergenic | 45.0% | |
| ATGTTAGTCCAGCTTCACGA+AGG | + | chr7.2:81079761-81079780 | None:intergenic | 45.0% | |
| CGGAATTTGTGGACATCAAG+CGG | + | chr7.2:81078762-81078781 | None:intergenic | 45.0% | |
| CGTAAAGCTCAAAGAGCAGA+AGG | - | chr7.2:81078448-81078467 | MS.gene44723:CDS | 45.0% | |
| CTGTCCAAGTGAGTATCACT+AGG | + | chr7.2:81079591-81079610 | None:intergenic | 45.0% | |
| GATAGTGAAGGAGCCATTGA+TGG | - | chr7.2:81079732-81079751 | MS.gene44723:intron | 45.0% | |
| GCGCAAAACAACCGTTTCAA+TGG | + | chr7.2:81080123-81080142 | None:intergenic | 45.0% | |
| GGACAAGCACTATTTGGAGA+TGG | - | chr7.2:81079618-81079637 | MS.gene44723:intron | 45.0% | |
| GTCTTCGCCCATATGTGAAA+AGG | - | chr7.2:81079433-81079452 | MS.gene44723:intron | 45.0% | |
| TCAATGGCTCCTTCACTATC+AGG | + | chr7.2:81079732-81079751 | None:intergenic | 45.0% | |
| TTCACGAAGGTGACCATCAA+TGG | + | chr7.2:81079748-81079767 | None:intergenic | 45.0% | |
| ! | ACGATACGAAAGCACACAAG+CGG | + | chr7.2:81078782-81078801 | None:intergenic | 45.0% |
| ! | ATCTAGAATTGCAGGTCCAC+CGG | + | chr7.2:81079901-81079920 | None:intergenic | 45.0% |
| !! | GGTTTCTCAATTTCTGGTGC+TGG | + | chr7.2:81079672-81079691 | None:intergenic | 45.0% |
| !! | TGGTAGAGGTACCTAGACTA+GGG | - | chr7.2:81079283-81079302 | MS.gene44723:CDS | 45.0% |
| !!! | TACCAACAAGGGTGTTTTGC+AGG | - | chr7.2:81079465-81079484 | MS.gene44723:intron | 45.0% |
| ACAAGTGGTGTAGACATGCC+TGG | - | chr7.2:81079381-81079400 | MS.gene44723:CDS | 50.0% | |
| ACGGGAGAAGGACTTGAATG+GGG | - | chr7.2:81080068-81080087 | MS.gene44723:CDS | 50.0% | |
| AGGCATGTCTACACCACTTG+TGG | + | chr7.2:81079382-81079401 | None:intergenic | 50.0% | |
| CAACGGGAGAAGGACTTGAA+TGG | - | chr7.2:81080066-81080085 | MS.gene44723:CDS | 50.0% | |
| CAAGGGTGGTAATAGCTTCG+TGG | + | chr7.2:81078683-81078702 | None:intergenic | 50.0% | |
| CACCTGCAAAACACCCTTGT+TGG | + | chr7.2:81079470-81079489 | None:intergenic | 50.0% | |
| CACGAAGCTATTACCACCCT+TGG | - | chr7.2:81078681-81078700 | MS.gene44723:CDS | 50.0% | |
| CATGTCTTGCCTAGCATCCA+AGG | + | chr7.2:81079259-81079278 | None:intergenic | 50.0% | |
| CCAATGGCCAAGATAGTTGC+AGG | + | chr7.2:81078475-81078494 | None:intergenic | 50.0% | |
| CCTGCAACTATCTTGGCCAT+TGG | - | chr7.2:81078472-81078491 | MS.gene44723:CDS | 50.0% | |
| CTTCGTGGGTTTCTCATGCA+TGG | + | chr7.2:81078668-81078687 | None:intergenic | 50.0% | |
| CTTGGATGCTAGGCAAGACA+TGG | - | chr7.2:81079257-81079276 | MS.gene44723:CDS | 50.0% | |
| GCGGTGGAAAGGAAAACATG+GGG | + | chr7.2:81078743-81078762 | None:intergenic | 50.0% | |
| GGATTGAAGACAACGGGAGA+AGG | - | chr7.2:81080056-81080075 | MS.gene44723:CDS | 50.0% | |
| TCACCGCTGTCACATTTCGT+GGG | - | chr7.2:81079565-81079584 | MS.gene44723:intron | 50.0% | |
| TCTTGCCTAGCATCCAAGGA+AGG | + | chr7.2:81079255-81079274 | None:intergenic | 50.0% | |
| TGCAGGATTTGCAGTGCCAA+TGG | + | chr7.2:81078491-81078510 | None:intergenic | 50.0% | |
| TGGGCCTAGTGATACTCACT+TGG | - | chr7.2:81079584-81079603 | MS.gene44723:intron | 50.0% | |
| TGGGCGAAGACCTAAGAGTT+TGG | + | chr7.2:81079424-81079443 | None:intergenic | 50.0% | |
| ! | ACTCACTTGGACAGCCTTGT+TGG | - | chr7.2:81079597-81079616 | MS.gene44723:intron | 50.0% |
| ! | TCTAGAATTGCAGGTCCACC+GGG | + | chr7.2:81079900-81079919 | None:intergenic | 50.0% |
| ! | TTTTGGATTGCACATCCCGG+TGG | - | chr7.2:81079882-81079901 | MS.gene44723:intron | 50.0% |
| !! | AGAGGTACCTAGACTAGGGA+AGG | - | chr7.2:81079287-81079306 | MS.gene44723:CDS | 50.0% |
| !! | CAACAAAGGTGCTCGTGTGT+TGG | - | chr7.2:81079527-81079546 | MS.gene44723:intron | 50.0% |
| !! | GTGAGTTGATAATCGGCTCC+AGG | + | chr7.2:81079402-81079421 | None:intergenic | 50.0% |
| !! | GTGGTAGAGGTACCTAGACT+AGG | - | chr7.2:81079282-81079301 | MS.gene44723:CDS | 50.0% |
| !!! | CAACAAGGGTGTTTTGCAGG+TGG | - | chr7.2:81079468-81079487 | MS.gene44723:intron | 50.0% |
| AGGCCCACGAAATGTGACAG+CGG | + | chr7.2:81079571-81079590 | None:intergenic | 55.0% | |
| GATGGTCACCTTCGTGAAGC+TGG | - | chr7.2:81079750-81079769 | MS.gene44723:intron | 55.0% | |
| GGAGCTGCTGCACTCATTGT+TGG | - | chr7.2:81079639-81079658 | MS.gene44723:intron | 55.0% | |
| GGATGCTAGGCAAGACATGG+TGG | - | chr7.2:81079260-81079279 | MS.gene44723:CDS | 55.0% | |
| GGTACCTAGACTAGGGAAGG+AGG | - | chr7.2:81079290-81079309 | MS.gene44723:CDS | 55.0% | |
| GTCACCGCTGTCACATTTCG+TGG | - | chr7.2:81079564-81079583 | MS.gene44723:intron | 55.0% | |
| GTCGAGGCTTTCGAGCCATT+GGG | - | chr7.2:81079837-81079856 | MS.gene44723:intron | 55.0% | |
| GTGGACATCAAGCGGTGGAA+AGG | + | chr7.2:81078754-81078773 | None:intergenic | 55.0% | |
| TGCTAGGCAAGACATGGTGG+TGG | - | chr7.2:81079263-81079282 | MS.gene44723:CDS | 55.0% | |
| TGGCACCTTCCTTGGATGCT+AGG | - | chr7.2:81079247-81079266 | MS.gene44723:CDS | 55.0% | |
| !! | AACGGTTGTTTTGCGCAGCG+TGG | - | chr7.2:81080127-81080146 | MS.gene44723:CDS | 55.0% |
| !! | AGGGTGTTTTGCAGGTGGCA+CGG | - | chr7.2:81079473-81079492 | MS.gene44723:intron | 55.0% |
| !! | GCAAGACATGGTGGTGGTAG+AGG | - | chr7.2:81079269-81079288 | MS.gene44723:CDS | 55.0% |
| AGGGAAGGAGGCTGCAGTGA+AGG | - | chr7.2:81079302-81079321 | MS.gene44723:CDS | 60.0% | |
| AGGTGGCACGGTGCTTCGTT+TGG | - | chr7.2:81079485-81079504 | MS.gene44723:intron | 60.0% | |
| GCAGCCTCCTTCCCTAGTCT+AGG | + | chr7.2:81079297-81079316 | None:intergenic | 60.0% | |
| GGTCGAGGCTTTCGAGCCAT+TGG | - | chr7.2:81079836-81079855 | MS.gene44723:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 81078427 | 81080157 | 81078427 | ID=MS.gene44723 |
| chr7.2 | mRNA | 81078427 | 81080157 | 81078427 | ID=MS.gene44723.t1;Parent=MS.gene44723 |
| chr7.2 | exon | 81079980 | 81080157 | 81079980 | ID=MS.gene44723.t1.exon1;Parent=MS.gene44723.t1 |
| chr7.2 | CDS | 81079980 | 81080157 | 81079980 | ID=cds.MS.gene44723.t1;Parent=MS.gene44723.t1 |
| chr7.2 | exon | 81078427 | 81079418 | 81078427 | ID=MS.gene44723.t1.exon2;Parent=MS.gene44723.t1 |
| chr7.2 | CDS | 81078427 | 81079418 | 81078427 | ID=cds.MS.gene44723.t1;Parent=MS.gene44723.t1 |
| Gene Sequence |
| Protein sequence |
