Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065465.t1 | XP_003624398.1 | 97.6 | 125 | 3 | 0 | 1 | 125 | 2 | 126 | 2.70E-60 | 241.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065465.t1 | G7KV85 | 97.6 | 125 | 3 | 0 | 1 | 125 | 2 | 126 | 2.0e-60 | 241.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene065465 | -0.800719 | 1.26E-48 | -1.69E-46 |
| MS.gene049281 | MS.gene065465 | 0.815533 | 8.80E-52 | -1.69E-46 |
| MS.gene049312 | MS.gene065465 | 0.812843 | 3.46E-51 | -1.69E-46 |
| MS.gene049611 | MS.gene065465 | 0.818581 | 1.82E-52 | -1.69E-46 |
| MS.gene050216 | MS.gene065465 | -0.801521 | 8.66E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene065465 | 0.803621 | 3.19E-49 | -1.69E-46 |
| MS.gene050380 | MS.gene065465 | 0.849803 | 2.56E-60 | -1.69E-46 |
| MS.gene050381 | MS.gene065465 | 0.895091 | 1.33E-75 | -1.69E-46 |
| MS.gene050504 | MS.gene065465 | -0.809386 | 1.94E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene065465 | -0.817755 | 2.80E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene065465 | -0.819896 | 9.13E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene065465 | 0.895848 | 6.49E-76 | -1.69E-46 |
| MS.gene05104 | MS.gene065465 | 0.823366 | 1.44E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene065465 | 0.834979 | 2.19E-56 | -1.69E-46 |
| MS.gene051364 | MS.gene065465 | 0.80829 | 3.33E-50 | -1.69E-46 |
| MS.gene05141 | MS.gene065465 | 0.852192 | 5.43E-61 | -1.69E-46 |
| MS.gene05142 | MS.gene065465 | 0.805635 | 1.21E-49 | -1.69E-46 |
| MS.gene051694 | MS.gene065465 | -0.82132 | 4.30E-53 | -1.69E-46 |
| MS.gene051946 | MS.gene065465 | 0.868844 | 4.83E-66 | -1.69E-46 |
| MS.gene052164 | MS.gene065465 | -0.817856 | 2.65E-52 | -1.69E-46 |
| MS.gene052329 | MS.gene065465 | 0.812771 | 3.59E-51 | -1.69E-46 |
| MS.gene05234 | MS.gene065465 | 0.832549 | 8.87E-56 | -1.69E-46 |
| MS.gene052482 | MS.gene065465 | -0.81362 | 2.33E-51 | -1.69E-46 |
| MS.gene052486 | MS.gene065465 | -0.816543 | 5.24E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene065465 | -0.817155 | 3.82E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene065465 | 0.844456 | 7.48E-59 | -1.69E-46 |
| MS.gene053436 | MS.gene065465 | -0.814655 | 1.38E-51 | -1.69E-46 |
| MS.gene053549 | MS.gene065465 | 0.835267 | 1.85E-56 | -1.69E-46 |
| MS.gene053561 | MS.gene065465 | 0.806897 | 6.58E-50 | -1.69E-46 |
| MS.gene053826 | MS.gene065465 | 0.847859 | 8.86E-60 | -1.69E-46 |
| MS.gene054003 | MS.gene065465 | -0.824873 | 6.37E-54 | -1.69E-46 |
| MS.gene054595 | MS.gene065465 | -0.818889 | 1.55E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene065465 | 0.80554 | 1.27E-49 | -1.69E-46 |
| MS.gene054707 | MS.gene065465 | 0.80539 | 1.37E-49 | -1.69E-46 |
| MS.gene054708 | MS.gene065465 | 0.840683 | 7.50E-58 | -1.69E-46 |
| MS.gene055124 | MS.gene065465 | 0.830416 | 2.97E-55 | -1.69E-46 |
| MS.gene055139 | MS.gene065465 | 0.84434 | 8.04E-59 | -1.69E-46 |
| MS.gene055154 | MS.gene065465 | 0.838261 | 3.19E-57 | -1.69E-46 |
| MS.gene055202 | MS.gene065465 | 0.830014 | 3.73E-55 | -1.69E-46 |
| MS.gene055401 | MS.gene065465 | 0.807555 | 4.77E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene065465 | -0.812248 | 4.66E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene065465 | -0.805362 | 1.38E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene065465 | 0.90647 | 1.43E-80 | -1.69E-46 |
| MS.gene056311 | MS.gene065465 | 0.909688 | 4.32E-82 | -1.69E-46 |
| MS.gene056313 | MS.gene065465 | 0.901682 | 2.09E-78 | -1.69E-46 |
| MS.gene056408 | MS.gene065465 | 0.832306 | 1.02E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene065465 | -0.806783 | 6.95E-50 | -1.69E-46 |
| MS.gene056647 | MS.gene065465 | 0.815952 | 7.10E-52 | -1.69E-46 |
| MS.gene057014 | MS.gene065465 | 0.871589 | 6.09E-67 | -1.69E-46 |
| MS.gene057173 | MS.gene065465 | 0.805066 | 1.60E-49 | -1.69E-46 |
| MS.gene05726 | MS.gene065465 | 0.835294 | 1.82E-56 | -1.69E-46 |
| MS.gene057499 | MS.gene065465 | 0.805657 | 1.20E-49 | -1.69E-46 |
| MS.gene057506 | MS.gene065465 | 0.805239 | 1.47E-49 | -1.69E-46 |
| MS.gene058068 | MS.gene065465 | 0.811528 | 6.69E-51 | -1.69E-46 |
| MS.gene058099 | MS.gene065465 | 0.836156 | 1.10E-56 | -1.69E-46 |
| MS.gene058108 | MS.gene065465 | 0.810791 | 9.67E-51 | -1.69E-46 |
| MS.gene058109 | MS.gene065465 | 0.845639 | 3.58E-59 | -1.69E-46 |
| MS.gene058111 | MS.gene065465 | 0.837566 | 4.82E-57 | -1.69E-46 |
| MS.gene058157 | MS.gene065465 | -0.857855 | 1.23E-62 | -1.69E-46 |
| MS.gene058170 | MS.gene065465 | 0.827635 | 1.40E-54 | -1.69E-46 |
| MS.gene058171 | MS.gene065465 | 0.851968 | 6.28E-61 | -1.69E-46 |
| MS.gene058172 | MS.gene065465 | 0.805289 | 1.43E-49 | -1.69E-46 |
| MS.gene058173 | MS.gene065465 | 0.84753 | 1.09E-59 | -1.69E-46 |
| MS.gene058174 | MS.gene065465 | 0.826693 | 2.36E-54 | -1.69E-46 |
| MS.gene058175 | MS.gene065465 | 0.849612 | 2.89E-60 | -1.69E-46 |
| MS.gene058180 | MS.gene065465 | 0.805873 | 1.08E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene065465 | -0.814176 | 1.76E-51 | -1.69E-46 |
| MS.gene058493 | MS.gene065465 | 0.852115 | 5.70E-61 | -1.69E-46 |
| MS.gene058494 | MS.gene065465 | 0.844449 | 7.51E-59 | -1.69E-46 |
| MS.gene058630 | MS.gene065465 | 0.801603 | 8.33E-49 | -1.69E-46 |
| MS.gene059037 | MS.gene065465 | 0.804505 | 2.09E-49 | -1.69E-46 |
| MS.gene059045 | MS.gene065465 | 0.808865 | 2.51E-50 | -1.69E-46 |
| MS.gene059223 | MS.gene065465 | 0.851632 | 7.82E-61 | -1.69E-46 |
| MS.gene059236 | MS.gene065465 | 0.842211 | 2.97E-58 | -1.69E-46 |
| MS.gene059367 | MS.gene065465 | -0.800269 | 1.56E-48 | -1.69E-46 |
| MS.gene059697 | MS.gene065465 | 0.804602 | 2.00E-49 | -1.69E-46 |
| MS.gene060187 | MS.gene065465 | 0.835282 | 1.84E-56 | -1.69E-46 |
| MS.gene060482 | MS.gene065465 | -0.845684 | 3.48E-59 | -1.69E-46 |
| MS.gene060595 | MS.gene065465 | 0.828033 | 1.13E-54 | -1.69E-46 |
| MS.gene060778 | MS.gene065465 | -0.801973 | 6.99E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene065465 | -0.834525 | 2.85E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065465.t1 | MTR_7g082760 | 97.600 | 125 | 3 | 0 | 1 | 125 | 2 | 126 | 6.16e-87 | 249 |
| MS.gene065465.t1 | MTR_7g082750 | 50.370 | 135 | 48 | 5 | 4 | 125 | 1 | 129 | 7.64e-36 | 119 |
| MS.gene065465.t1 | MTR_7g082740 | 52.756 | 127 | 45 | 4 | 14 | 125 | 4 | 130 | 6.06e-34 | 115 |
| MS.gene065465.t1 | MTR_7g082700 | 52.756 | 127 | 45 | 4 | 14 | 125 | 4 | 130 | 1.30e-33 | 114 |
| MS.gene065465.t1 | MTR_7g082730 | 55.200 | 125 | 41 | 5 | 15 | 125 | 5 | 128 | 4.50e-33 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTTGTCCACATCTTTAT+TGG | 0.171024 | 7.3:+25106109 | None:intergenic |
| CCGTAAGTACCTCGTTGGTT+TGG | 0.301020 | 7.3:+25106261 | None:intergenic |
| ACTTATATTTGAGATTTCTA+AGG | 0.310038 | 7.3:-25105972 | MS.gene065465:CDS |
| AGAAGGATTAAACTATTTCA+TGG | 0.325765 | 7.3:+25105922 | None:intergenic |
| CAAGGTTAACATGGGCTATT+AGG | 0.336227 | 7.3:-25106135 | MS.gene065465:CDS |
| ATGAAAAGTGATACCATCTT+TGG | 0.337936 | 7.3:-25106052 | MS.gene065465:CDS |
| CGTAAGTACCTCGTTGGTTT+GGG | 0.339937 | 7.3:+25106262 | None:intergenic |
| TAAGGATCGTGTAGTTTCTA+AGG | 0.360505 | 7.3:-25106026 | MS.gene065465:CDS |
| ATTTGAGATTTCTAAGGCCT+TGG | 0.387808 | 7.3:-25105966 | MS.gene065465:CDS |
| CATAATGAAAAGCGTGGAAA+TGG | 0.389387 | 7.3:-25106296 | None:intergenic |
| GAAGGAAGACCAAGGTTCTA+AGG | 0.400859 | 7.3:-25106192 | MS.gene065465:CDS |
| TGATACCATCTTTGGAACTA+AGG | 0.464934 | 7.3:-25106044 | MS.gene065465:CDS |
| TCTTCTCAACCTTAGAACCT+TGG | 0.471764 | 7.3:+25106183 | None:intergenic |
| AGGTTCTAAGGTTGAGAAGA+AGG | 0.473602 | 7.3:-25106180 | MS.gene065465:CDS |
| CCAAACCAACGAGGTACTTA+CGG | 0.478482 | 7.3:-25106261 | MS.gene065465:CDS |
| CTTCCTTCCATCACCGGTGA+TGG | 0.490534 | 7.3:+25106207 | None:intergenic |
| CGCCTCCGTAAGTACCTCGT+TGG | 0.498443 | 7.3:+25106256 | None:intergenic |
| CTTGGTCTTCCTTCCATCAC+CGG | 0.498548 | 7.3:+25106201 | None:intergenic |
| GAGTAGTTCCAAGGTTAACA+TGG | 0.518857 | 7.3:-25106144 | MS.gene065465:CDS |
| GAGGATGCCATCACCGGTGA+TGG | 0.521516 | 7.3:-25106214 | MS.gene065465:CDS |
| CTAATAGCCCATGTTAACCT+TGG | 0.534159 | 7.3:+25106136 | None:intergenic |
| CTCGACGAGGATGCCATCAC+CGG | 0.557338 | 7.3:-25106220 | MS.gene065465:CDS |
| TAAGGTTGAGAAGAAGGCAG+TGG | 0.557373 | 7.3:-25106174 | MS.gene065465:CDS |
| ACGATCCTTAGTTCCAAAGA+TGG | 0.563624 | 7.3:+25106039 | None:intergenic |
| ATGCCATCACCGGTGATGGA+AGG | 0.564522 | 7.3:-25106210 | MS.gene065465:CDS |
| GGTTGAGAAGAAGGCAGTGG+AGG | 0.585451 | 7.3:-25106171 | MS.gene065465:CDS |
| CTTCATCATAATGAAAAGCG+TGG | 0.588975 | 7.3:-25106302 | None:intergenic |
| GGAGGATAAGAGTAGTTCCA+AGG | 0.595694 | 7.3:-25106153 | MS.gene065465:CDS |
| CAACGAGGTACTTACGGAGG+CGG | 0.621368 | 7.3:-25106255 | MS.gene065465:CDS |
| AGTAGTTCCAAGGTTAACAT+GGG | 0.641526 | 7.3:-25106143 | MS.gene065465:CDS |
| TAATTCATGAGAAGCAACCA+AGG | 0.645785 | 7.3:+25105949 | None:intergenic |
| TTAGGTCACCAATAAAGATG+TGG | 0.652661 | 7.3:-25106117 | MS.gene065465:CDS |
| GCGATATCAGCGACTCGACG+AGG | 0.678608 | 7.3:-25106233 | MS.gene065465:CDS |
| CGGTGATGGAAGGAAGACCA+AGG | 0.711559 | 7.3:-25106200 | MS.gene065465:CDS |
| AACCAACGAGGTACTTACGG+AGG | 0.715584 | 7.3:-25106258 | MS.gene065465:CDS |
| CACTGATACCCAAACCAACG+AGG | 0.721556 | 7.3:-25106270 | MS.gene065465:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTATATTTGAGATTTCTA+AGG | - | chr7.3:25106254-25106273 | MS.gene065465:CDS | 20.0% |
| ATGAAAAGTGATACCATCTT+TGG | - | chr7.3:25106174-25106193 | MS.gene065465:CDS | 30.0% | |
| !! | AAAGATGGTATCACTTTTCA+TGG | + | chr7.3:25106175-25106194 | None:intergenic | 30.0% |
| AGTAGTTCCAAGGTTAACAT+GGG | - | chr7.3:25106083-25106102 | MS.gene065465:CDS | 35.0% | |
| TAAGGATCGTGTAGTTTCTA+AGG | - | chr7.3:25106200-25106219 | MS.gene065465:CDS | 35.0% | |
| TAATTCATGAGAAGCAACCA+AGG | + | chr7.3:25106280-25106299 | None:intergenic | 35.0% | |
| TTAGGTCACCAATAAAGATG+TGG | - | chr7.3:25106109-25106128 | MS.gene065465:CDS | 35.0% | |
| ! | AGCTTTGTCCACATCTTTAT+TGG | + | chr7.3:25106120-25106139 | None:intergenic | 35.0% |
| ! | TTATTCTCGTGATGCTTTTG+AGG | - | chr7.3:25106227-25106246 | MS.gene065465:CDS | 35.0% |
| !! | ATTTGAGATTTCTAAGGCCT+TGG | - | chr7.3:25106260-25106279 | MS.gene065465:CDS | 35.0% |
| !! | TGATACCATCTTTGGAACTA+AGG | - | chr7.3:25106182-25106201 | MS.gene065465:CDS | 35.0% |
| ACGATCCTTAGTTCCAAAGA+TGG | + | chr7.3:25106190-25106209 | None:intergenic | 40.0% | |
| CTAATAGCCCATGTTAACCT+TGG | + | chr7.3:25106093-25106112 | None:intergenic | 40.0% | |
| GAGTAGTTCCAAGGTTAACA+TGG | - | chr7.3:25106082-25106101 | MS.gene065465:CDS | 40.0% | |
| TCTTCTCAACCTTAGAACCT+TGG | + | chr7.3:25106046-25106065 | None:intergenic | 40.0% | |
| ! | CAAGGTTAACATGGGCTATT+AGG | - | chr7.3:25106091-25106110 | MS.gene065465:CDS | 40.0% |
| !! | AGGTTCTAAGGTTGAGAAGA+AGG | - | chr7.3:25106046-25106065 | MS.gene065465:CDS | 40.0% |
| GAAGGAAGACCAAGGTTCTA+AGG | - | chr7.3:25106034-25106053 | MS.gene065465:CDS | 45.0% | |
| GGAGGATAAGAGTAGTTCCA+AGG | - | chr7.3:25106073-25106092 | MS.gene065465:CDS | 45.0% | |
| TAAGGTTGAGAAGAAGGCAG+TGG | - | chr7.3:25106052-25106071 | MS.gene065465:CDS | 45.0% | |
| ! | CCAAACCAACGAGGTACTTA+CGG | - | chr7.3:25105965-25105984 | MS.gene065465:CDS | 45.0% |
| !! | CGTAAGTACCTCGTTGGTTT+GGG | + | chr7.3:25105967-25105986 | None:intergenic | 45.0% |
| CACTGATACCCAAACCAACG+AGG | - | chr7.3:25105956-25105975 | MS.gene065465:CDS | 50.0% | |
| ! | AACCAACGAGGTACTTACGG+AGG | - | chr7.3:25105968-25105987 | MS.gene065465:CDS | 50.0% |
| ! | CCGTAAGTACCTCGTTGGTT+TGG | + | chr7.3:25105968-25105987 | None:intergenic | 50.0% |
| ! | CTTGGTCTTCCTTCCATCAC+CGG | + | chr7.3:25106028-25106047 | None:intergenic | 50.0% |
| CTTCCTTCCATCACCGGTGA+TGG | + | chr7.3:25106022-25106041 | None:intergenic | 55.0% | |
| GGTTGAGAAGAAGGCAGTGG+AGG | - | chr7.3:25106055-25106074 | MS.gene065465:CDS | 55.0% | |
| ! | CAACGAGGTACTTACGGAGG+CGG | - | chr7.3:25105971-25105990 | MS.gene065465:CDS | 55.0% |
| !! | ATGCCATCACCGGTGATGGA+AGG | - | chr7.3:25106016-25106035 | MS.gene065465:CDS | 55.0% |
| !! | CGGTGATGGAAGGAAGACCA+AGG | - | chr7.3:25106026-25106045 | MS.gene065465:CDS | 55.0% |
| CGCCTCCGTAAGTACCTCGT+TGG | + | chr7.3:25105973-25105992 | None:intergenic | 60.0% | |
| CTCGACGAGGATGCCATCAC+CGG | - | chr7.3:25106006-25106025 | MS.gene065465:CDS | 60.0% | |
| GAGGATGCCATCACCGGTGA+TGG | - | chr7.3:25106012-25106031 | MS.gene065465:CDS | 60.0% | |
| GCGATATCAGCGACTCGACG+AGG | - | chr7.3:25105993-25106012 | MS.gene065465:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 25105934 | 25106314 | 25105934 | ID=MS.gene065465 |
| chr7.3 | mRNA | 25105934 | 25106314 | 25105934 | ID=MS.gene065465.t1;Parent=MS.gene065465 |
| chr7.3 | exon | 25105934 | 25106314 | 25105934 | ID=MS.gene065465.t1.exon1;Parent=MS.gene065465.t1 |
| chr7.3 | CDS | 25105934 | 25106314 | 25105934 | ID=cds.MS.gene065465.t1;Parent=MS.gene065465.t1 |
| Gene Sequence |
| Protein sequence |
