Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051915.t1 | GAU47991.1 | 72.9 | 229 | 53 | 1 | 1 | 229 | 129 | 348 | 6.70E-89 | 337 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051915.t1 | Q84WU2 | 40.3 | 72 | 42 | 1 | 4 | 75 | 112 | 182 | 3.8e-08 | 60.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051915.t1 | A0A2Z6NUX7 | 72.9 | 229 | 53 | 1 | 1 | 229 | 129 | 348 | 4.8e-89 | 337.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene030033 | MS.gene051915 | PPI |
| MS.gene038328 | MS.gene051915 | PPI |
| MS.gene057090 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene057065 | PPI |
| MS.gene038336 | MS.gene051915 | PPI |
| MS.gene057066 | MS.gene051915 | PPI |
| MS.gene030037 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene038324 | PPI |
| MS.gene017099 | MS.gene051915 | PPI |
| MS.gene057069 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene030042 | PPI |
| MS.gene038323 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene017101 | PPI |
| MS.gene038333 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene017115 | PPI |
| MS.gene051915 | MS.gene038327 | PPI |
| MS.gene030029 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene057070 | PPI |
| MS.gene051915 | MS.gene030045 | PPI |
| MS.gene038330 | MS.gene051915 | PPI |
| MS.gene017102 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene017100 | PPI |
| MS.gene038342 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene030026 | PPI |
| MS.gene030034 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene038342 | PPI |
| MS.gene038300 | MS.gene051915 | PPI |
| MS.gene051915 | MS.gene038326 | PPI |
| MS.gene051915 | MS.gene017083 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051915.t1 | MTR_7g107160 | 77.391 | 230 | 22 | 3 | 1 | 229 | 193 | 393 | 4.17e-109 | 321 |
| MS.gene051915.t1 | MTR_7g107160 | 49.550 | 111 | 55 | 1 | 1 | 111 | 1 | 110 | 6.54e-27 | 107 |
| MS.gene051915.t1 | MTR_7g107200 | 47.059 | 255 | 105 | 3 | 3 | 228 | 72 | 325 | 3.69e-69 | 217 |
| MS.gene051915.t1 | MTR_1g042160 | 42.358 | 229 | 95 | 5 | 1 | 229 | 69 | 260 | 7.69e-50 | 165 |
| MS.gene051915.t1 | MTR_8g011730 | 37.154 | 253 | 126 | 6 | 2 | 229 | 72 | 316 | 1.55e-48 | 163 |
| MS.gene051915.t1 | MTR_7g107170 | 59.091 | 110 | 37 | 1 | 120 | 229 | 114 | 215 | 3.08e-36 | 129 |
| MS.gene051915.t1 | MTR_7g107170 | 81.250 | 48 | 9 | 0 | 182 | 229 | 1 | 48 | 2.16e-20 | 87.0 |
| MS.gene051915.t1 | MTR_2g034780 | 48.544 | 103 | 50 | 1 | 127 | 229 | 119 | 218 | 1.23e-25 | 101 |
| MS.gene051915.t1 | MTR_5g091450 | 37.500 | 72 | 44 | 1 | 1 | 72 | 88 | 158 | 3.00e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051915.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 110 | 183 | 1.26e-11 | 64.3 |
| MS.gene051915.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 110 | 183 | 1.27e-11 | 64.3 |
| MS.gene051915.t1 | AT5G06600 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 1.27e-11 | 64.3 |
| MS.gene051915.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 4.77e-11 | 62.4 |
| MS.gene051915.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 109 | 182 | 4.77e-11 | 62.4 |
| MS.gene051915.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 108 | 181 | 5.05e-11 | 62.4 |
| MS.gene051915.t1 | AT3G11910 | 38.667 | 75 | 45 | 1 | 1 | 75 | 108 | 181 | 5.05e-11 | 62.4 |
Find 43 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAAGAAGTTGTAAGTTTA+GGG | 0.193564 | 7.1:+6034167 | MS.gene051915:CDS |
| AGAAAAGAAGTTGTAAGTTT+AGG | 0.206519 | 7.1:+6034166 | MS.gene051915:CDS |
| TGGCTGGGGTATTGGATCTT+TGG | 0.242019 | 7.1:+6033825 | MS.gene051915:CDS |
| GGAATGGGCGTTCACTGCTT+TGG | 0.299493 | 7.1:+6034188 | MS.gene051915:CDS |
| CTTATGGATTTCAAGGGTTT+AGG | 0.322047 | 7.1:+6034069 | MS.gene051915:CDS |
| GCAAAATCCAGGTGAACTTA+TGG | 0.322911 | 7.1:+6034053 | MS.gene051915:CDS |
| GTAAAAGATGTTTGTATTGT+TGG | 0.326070 | 7.1:+6033889 | MS.gene051915:CDS |
| AGCTCCAATGGCTGGGGTAT+TGG | 0.327929 | 7.1:+6033817 | MS.gene051915:CDS |
| GAATGGGCGTTCACTGCTTT+GGG | 0.331310 | 7.1:+6034189 | MS.gene051915:CDS |
| TAGAGGTCAAACTGAAAATC+TGG | 0.352353 | 7.1:+6033990 | MS.gene051915:CDS |
| TGGGAGGAACTTCAGCCATT+TGG | 0.359556 | 7.1:+6034288 | MS.gene051915:CDS |
| TCACTGCAAGCTCCAATGGC+TGG | 0.398816 | 7.1:+6033809 | MS.gene051915:CDS |
| CACTGCAAGCTCCAATGGCT+GGG | 0.419346 | 7.1:+6033810 | MS.gene051915:CDS |
| TATGAATGACTTATCTTGTA+AGG | 0.428376 | 7.1:+6034251 | MS.gene051915:CDS |
| CCAGGAACTTCTTCTAGCAT+TGG | 0.429065 | 7.1:-6034030 | None:intergenic |
| AGGTGAACTTATGGATTTCA+AGG | 0.444860 | 7.1:+6034062 | MS.gene051915:CDS |
| GTTCTTCATGATCCTCATAG+TGG | 0.455915 | 7.1:+6033859 | MS.gene051915:CDS |
| CCAATGCTAGAAGAAGTTCC+TGG | 0.456723 | 7.1:+6034030 | MS.gene051915:CDS |
| ATCTGGAAGCTGAAGTCATA+AGG | 0.473546 | 7.1:+6034007 | MS.gene051915:CDS |
| AAGTTGTAAGTTTAGGGAAT+GGG | 0.480762 | 7.1:+6034173 | MS.gene051915:CDS |
| GAAGTTGTAAGTTTAGGGAA+TGG | 0.491256 | 7.1:+6034172 | MS.gene051915:CDS |
| AGTTTGACCTCTAGATGTAA+AGG | 0.494100 | 7.1:-6033980 | None:intergenic |
| GAAGTTCCTGGGCAAAATCC+AGG | 0.501134 | 7.1:+6034042 | MS.gene051915:CDS |
| TTCTTCATGATCCTCATAGT+GGG | 0.502613 | 7.1:+6033860 | MS.gene051915:CDS |
| TGCTGGGACTCAACAAGTGA+AGG | 0.505102 | 7.1:-6034144 | None:intergenic |
| GAACACTCTTCTACTAGTAA+TGG | 0.512065 | 7.1:-6034114 | None:intergenic |
| AGATCCAATACCCCAGCCAT+TGG | 0.519689 | 7.1:-6033821 | None:intergenic |
| GAGTTCACTGCAAGCTCCAA+TGG | 0.520868 | 7.1:+6033805 | MS.gene051915:CDS |
| TTTACAAGAAACCCACTATG+AGG | 0.523336 | 7.1:-6033871 | None:intergenic |
| CAAACTGCTAACATGTCTAA+AGG | 0.546098 | 7.1:+6033538 | None:intergenic |
| ACTACTTCCTTTACATCTAG+AGG | 0.550454 | 7.1:+6033973 | MS.gene051915:CDS |
| CTGCTAACATGTCTAAAGGA+TGG | 0.552141 | 7.1:+6033542 | None:intergenic |
| TTGAAATCCATAAGTTCACC+TGG | 0.559661 | 7.1:-6034060 | None:intergenic |
| TGTTTGTATTGTTGGTGTAG+AGG | 0.561083 | 7.1:+6033897 | MS.gene051915:CDS |
| GTATCATGTTCAATCAGCCC+TGG | 0.564297 | 7.1:+6034332 | MS.gene051915:CDS |
| TTGTTCTGTTGCTGTCGACC+TGG | 0.565780 | 7.1:-6033600 | None:intergenic |
| TAAGTTGTTTGTTCTTAACC+AGG | 0.569810 | 7.1:+6033582 | MS.gene051915:CDS |
| GGTGAACTTATGGATTTCAA+GGG | 0.574063 | 7.1:+6034063 | MS.gene051915:CDS |
| AACAGAACAATCTTCTCTGA+TGG | 0.582000 | 7.1:+6033613 | MS.gene051915:CDS |
| CAATGCTAGAAGAAGTTCCT+GGG | 0.601828 | 7.1:+6034031 | MS.gene051915:CDS |
| ACTGCAAGCTCCAATGGCTG+GGG | 0.608500 | 7.1:+6033811 | MS.gene051915:CDS |
| TATCATGTTCAATCAGCCCT+GGG | 0.620754 | 7.1:+6034333 | MS.gene051915:CDS |
| GCTGGGACTCAACAAGTGAA+GGG | 0.631377 | 7.1:-6034143 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTATTTTTCTTATGG+TGG | + | chr7.1:6033640-6033659 | MS.gene051915:intron | 15.0% |
| !!! | TAAGGATCTTCAAATTTTTT+GGG | + | chr7.1:6034269-6034288 | MS.gene051915:CDS | 20.0% |
| ! | AGAAAAGAAGTTGTAAGTTT+AGG | + | chr7.1:6034166-6034185 | MS.gene051915:CDS | 25.0% |
| ! | CAAGACAAATAAAAACCAAA+TGG | - | chr7.1:6034306-6034325 | None:intergenic | 25.0% |
| ! | GAAAAGAAGTTGTAAGTTTA+GGG | + | chr7.1:6034167-6034186 | MS.gene051915:CDS | 25.0% |
| ! | GTAAAAGATGTTTGTATTGT+TGG | + | chr7.1:6033889-6033908 | MS.gene051915:CDS | 25.0% |
| ! | TATGAATGACTTATCTTGTA+AGG | + | chr7.1:6034251-6034270 | MS.gene051915:CDS | 25.0% |
| !!! | AAGTTTTTTGGAGAAAATGA+AGG | + | chr7.1:6034362-6034381 | MS.gene051915:CDS | 25.0% |
| !!! | CATTTGGTTTTTATTTGTCT+TGG | + | chr7.1:6034304-6034323 | MS.gene051915:CDS | 25.0% |
| !!! | GTAAGGATCTTCAAATTTTT+TGG | + | chr7.1:6034268-6034287 | MS.gene051915:CDS | 25.0% |
| AAGTTGTAAGTTTAGGGAAT+GGG | + | chr7.1:6034173-6034192 | MS.gene051915:CDS | 30.0% | |
| GAACAACACGTAAAAAGAAA+GGG | - | chr7.1:6033678-6033697 | None:intergenic | 30.0% | |
| TAAGTTGTTTGTTCTTAACC+AGG | + | chr7.1:6033582-6033601 | MS.gene051915:CDS | 30.0% | |
| TGAACAACACGTAAAAAGAA+AGG | - | chr7.1:6033679-6033698 | None:intergenic | 30.0% | |
| ! | CAAAAAACTTTTCATTCCCA+GGG | - | chr7.1:6034352-6034371 | None:intergenic | 30.0% |
| !!! | CTTTCACTTTTCTAGTCTTA+AGG | - | chr7.1:6034230-6034249 | None:intergenic | 30.0% |
| !!! | TTTTTTGGAGAAAATGAAGG+AGG | + | chr7.1:6034365-6034384 | MS.gene051915:CDS | 30.0% |
| AACAGAACAATCTTCTCTGA+TGG | + | chr7.1:6033613-6033632 | MS.gene051915:CDS | 35.0% | |
| ACTACTTCCTTTACATCTAG+AGG | + | chr7.1:6033973-6033992 | MS.gene051915:CDS | 35.0% | |
| AGGTGAACTTATGGATTTCA+AGG | + | chr7.1:6034062-6034081 | MS.gene051915:CDS | 35.0% | |
| AGTTTGACCTCTAGATGTAA+AGG | - | chr7.1:6033983-6034002 | None:intergenic | 35.0% | |
| CTTATGGATTTCAAGGGTTT+AGG | + | chr7.1:6034069-6034088 | MS.gene051915:CDS | 35.0% | |
| GAACACTCTTCTACTAGTAA+TGG | - | chr7.1:6034117-6034136 | None:intergenic | 35.0% | |
| GAAGTTGTAAGTTTAGGGAA+TGG | + | chr7.1:6034172-6034191 | MS.gene051915:CDS | 35.0% | |
| GGTGAACTTATGGATTTCAA+GGG | + | chr7.1:6034063-6034082 | MS.gene051915:CDS | 35.0% | |
| TAGAGGTCAAACTGAAAATC+TGG | + | chr7.1:6033990-6034009 | MS.gene051915:CDS | 35.0% | |
| TGTTTGTATTGTTGGTGTAG+AGG | + | chr7.1:6033897-6033916 | MS.gene051915:CDS | 35.0% | |
| TTCTTCATGATCCTCATAGT+GGG | + | chr7.1:6033860-6033879 | MS.gene051915:CDS | 35.0% | |
| TTGAAATCCATAAGTTCACC+TGG | - | chr7.1:6034063-6034082 | None:intergenic | 35.0% | |
| TTTACAAGAAACCCACTATG+AGG | - | chr7.1:6033874-6033893 | None:intergenic | 35.0% | |
| ! | ACTTACAACTTCTTTTCTGC+TGG | - | chr7.1:6034164-6034183 | None:intergenic | 35.0% |
| ! | CCAAAAAACTTTTCATTCCC+AGG | - | chr7.1:6034353-6034372 | None:intergenic | 35.0% |
| ! | CTTACAACTTCTTTTCTGCT+GGG | - | chr7.1:6034163-6034182 | None:intergenic | 35.0% |
| !!! | CCTGGGAATGAAAAGTTTTT+TGG | + | chr7.1:6034350-6034369 | MS.gene051915:CDS | 35.0% |
| !!! | GGATCTTCAAATTTTTTGGG+AGG | + | chr7.1:6034272-6034291 | MS.gene051915:CDS | 35.0% |
| !!! | TGATTCATGTTTTTTCCCAG+AGG | + | chr7.1:6033774-6033793 | MS.gene051915:intron | 35.0% |
| ATCTGGAAGCTGAAGTCATA+AGG | + | chr7.1:6034007-6034026 | MS.gene051915:CDS | 40.0% | |
| CAATGCTAGAAGAAGTTCCT+GGG | + | chr7.1:6034031-6034050 | MS.gene051915:CDS | 40.0% | |
| GCAAAATCCAGGTGAACTTA+TGG | + | chr7.1:6034053-6034072 | MS.gene051915:CDS | 40.0% | |
| GTTCTTCATGATCCTCATAG+TGG | + | chr7.1:6033859-6033878 | MS.gene051915:CDS | 40.0% | |
| TATCATGTTCAATCAGCCCT+GGG | + | chr7.1:6034333-6034352 | MS.gene051915:CDS | 40.0% | |
| CCAATGCTAGAAGAAGTTCC+TGG | + | chr7.1:6034030-6034049 | MS.gene051915:CDS | 45.0% | |
| CCAGGAACTTCTTCTAGCAT+TGG | - | chr7.1:6034033-6034052 | None:intergenic | 45.0% | |
| GTATCATGTTCAATCAGCCC+TGG | + | chr7.1:6034332-6034351 | MS.gene051915:CDS | 45.0% | |
| !!! | ATTTTTTTTTATTTTTCTTA+TGG | + | chr7.1:6033637-6033656 | MS.gene051915:intron | 5.0% |
| AGATCCAATACCCCAGCCAT+TGG | - | chr7.1:6033824-6033843 | None:intergenic | 50.0% | |
| GAAGTTCCTGGGCAAAATCC+AGG | + | chr7.1:6034042-6034061 | MS.gene051915:CDS | 50.0% | |
| GAATGGGCGTTCACTGCTTT+GGG | + | chr7.1:6034189-6034208 | MS.gene051915:CDS | 50.0% | |
| GAGTTCACTGCAAGCTCCAA+TGG | + | chr7.1:6033805-6033824 | MS.gene051915:CDS | 50.0% | |
| GCTGGGACTCAACAAGTGAA+GGG | - | chr7.1:6034146-6034165 | None:intergenic | 50.0% | |
| TGAACTCGTGTTGAGCCTCT+GGG | - | chr7.1:6033792-6033811 | None:intergenic | 50.0% | |
| TGCTGGGACTCAACAAGTGA+AGG | - | chr7.1:6034147-6034166 | None:intergenic | 50.0% | |
| TGGGAGGAACTTCAGCCATT+TGG | + | chr7.1:6034288-6034307 | MS.gene051915:CDS | 50.0% | |
| TTGTTCTGTTGCTGTCGACC+TGG | - | chr7.1:6033603-6033622 | None:intergenic | 50.0% | |
| ! | AGTTCACCTGGATTTTGCCC+AGG | - | chr7.1:6034051-6034070 | None:intergenic | 50.0% |
| !! | TGGCTGGGGTATTGGATCTT+TGG | + | chr7.1:6033825-6033844 | MS.gene051915:CDS | 50.0% |
| ACTGCAAGCTCCAATGGCTG+GGG | + | chr7.1:6033811-6033830 | MS.gene051915:CDS | 55.0% | |
| AGCTCCAATGGCTGGGGTAT+TGG | + | chr7.1:6033817-6033836 | MS.gene051915:CDS | 55.0% | |
| CACTGCAAGCTCCAATGGCT+GGG | + | chr7.1:6033810-6033829 | MS.gene051915:CDS | 55.0% | |
| GGAATGGGCGTTCACTGCTT+TGG | + | chr7.1:6034188-6034207 | MS.gene051915:CDS | 55.0% | |
| GTGAACTCGTGTTGAGCCTC+TGG | - | chr7.1:6033793-6033812 | None:intergenic | 55.0% | |
| TCACTGCAAGCTCCAATGGC+TGG | + | chr7.1:6033809-6033828 | MS.gene051915:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 6033550 | 6034398 | 6033550 | ID=MS.gene051915 |
| chr7.1 | mRNA | 6033550 | 6034398 | 6033550 | ID=MS.gene051915.t1;Parent=MS.gene051915 |
| chr7.1 | exon | 6033550 | 6033634 | 6033550 | ID=MS.gene051915.t1.exon1;Parent=MS.gene051915.t1 |
| chr7.1 | CDS | 6033550 | 6033634 | 6033550 | ID=cds.MS.gene051915.t1;Parent=MS.gene051915.t1 |
| chr7.1 | exon | 6033794 | 6034398 | 6033794 | ID=MS.gene051915.t1.exon2;Parent=MS.gene051915.t1 |
| chr7.1 | CDS | 6033794 | 6034398 | 6033794 | ID=cds.MS.gene051915.t1;Parent=MS.gene051915.t1 |
| Gene Sequence |
| Protein sequence |
