AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051920.t1 MTR_1g042160 55.172 348 115 4 1 347 1 308 2.14e-131 378
MS.gene051920.t1 MTR_7g107200 49.734 376 162 6 1 349 1 376 5.79e-123 359
MS.gene051920.t1 MTR_7g107160 45.856 362 143 10 2 347 119 443 1.29e-85 266
MS.gene051920.t1 MTR_7g107160 60.952 105 41 0 68 172 1 105 3.22e-36 136
MS.gene051920.t1 MTR_7g107170 73.620 163 41 1 193 353 107 269 2.70e-81 249
MS.gene051920.t1 MTR_7g107170 60.920 87 34 0 252 338 1 87 8.99e-26 104
MS.gene051920.t1 MTR_8g011730 36.712 365 185 11 11 350 12 355 7.29e-66 212
MS.gene051920.t1 MTR_3g079840 45.070 142 77 1 1 142 45 185 4.61e-39 148
MS.gene051920.t1 MTR_3g079840 45.070 142 77 1 1 142 44 184 4.69e-39 148
MS.gene051920.t1 MTR_5g091450 44.776 134 73 1 13 146 33 165 4.34e-38 145
MS.gene051920.t1 MTR_5g086700 46.154 130 69 1 13 142 61 189 2.62e-37 143
MS.gene051920.t1 MTR_5g086700 46.154 130 69 1 13 142 61 189 2.67e-37 143
MS.gene051920.t1 MTR_1g041240 44.853 136 73 2 6 141 50 183 6.82e-36 139
MS.gene051920.t1 MTR_1g041240 44.853 136 73 2 6 141 52 185 6.89e-36 139
MS.gene051920.t1 MTR_1g041240 44.853 136 73 2 6 141 51 184 7.02e-36 139
MS.gene051920.t1 MTR_5g091180 36.957 138 87 0 1 138 34 171 1.74e-26 110
MS.gene051920.t1 MTR_5g091550 38.583 127 77 1 12 138 18 143 1.75e-24 104
MS.gene051920.t1 MTR_5g091550 38.583 127 77 1 12 138 18 143 1.91e-24 104
MS.gene051920.t1 MTR_1g044450 35.075 134 82 3 9 141 61 190 1.79e-19 90.5
MS.gene051920.t1 MTR_4g123750 32.168 143 88 4 14 147 20 162 1.98e-18 85.1
MS.gene051920.t1 MTR_4g123750 28.571 140 97 2 6 143 169 307 5.62e-11 63.2
MS.gene051920.t1 MTR_1g073790 33.582 134 84 3 9 141 58 187 3.14e-18 86.7
MS.gene051920.t1 MTR_3g106700 33.333 141 84 2 13 143 406 546 7.75e-18 85.5
MS.gene051920.t1 MTR_3g106700 26.190 126 89 3 16 137 71 196 2.60e-11 65.5
MS.gene051920.t1 MTR_1g073800 32.090 134 88 3 9 141 45 176 7.86e-18 84.7
MS.gene051920.t1 MTR_2g034780 39.837 123 69 2 202 322 122 241 2.43e-17 81.3
MS.gene051920.t1 MTR_8g006190 34.783 115 69 2 31 139 10 124 3.88e-16 79.7
MS.gene051920.t1 MTR_8g006190 33.654 104 63 2 42 139 13 116 5.99e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051920.t1 AT5G06600 50.000 142 70 1 1 142 44 184 4.57e-41 154
MS.gene051920.t1 AT5G06600 50.000 142 70 1 1 142 43 183 5.49e-41 154
MS.gene051920.t1 AT5G06600 50.000 142 70 1 1 142 44 184 5.55e-41 154
MS.gene051920.t1 AT3G11910 50.769 130 63 1 13 142 55 183 4.02e-39 148
MS.gene051920.t1 AT3G11910 50.769 130 63 1 13 142 55 183 4.02e-39 148
MS.gene051920.t1 AT3G11910 50.769 130 63 1 13 142 54 182 4.51e-39 148
MS.gene051920.t1 AT3G11910 50.769 130 63 1 13 142 54 182 4.51e-39 148
MS.gene051920.t1 AT3G58270 40.146 137 81 1 6 142 1 136 2.92e-26 107
MS.gene051920.t1 AT3G58270 40.146 137 81 1 6 142 1 136 2.92e-26 107
MS.gene051920.t1 AT3G58270 40.146 137 81 1 6 142 1 136 2.92e-26 107
MS.gene051920.t1 AT3G58250 32.749 171 109 3 12 176 9 179 1.88e-24 102
MS.gene051920.t1 AT3G58370 35.135 148 94 1 8 153 4 151 1.09e-22 97.1
MS.gene051920.t1 AT3G58340 25.150 334 213 8 6 321 1 315 1.21e-22 97.4
MS.gene051920.t1 AT3G58340 33.577 137 90 1 6 142 1 136 7.65e-22 94.7
MS.gene051920.t1 AT3G58210 30.723 166 114 1 6 170 1 166 9.32e-22 94.7
MS.gene051920.t1 AT3G58410 34.848 132 82 2 6 137 1 128 9.86e-21 91.7
MS.gene051920.t1 AT3G58360 31.757 148 100 1 6 153 1 147 1.77e-20 90.9
MS.gene051920.t1 AT3G27040 31.429 140 96 0 12 151 8 147 3.03e-20 89.7
MS.gene051920.t1 AT3G58260 34.043 141 90 2 6 143 1 141 6.73e-20 89.4
MS.gene051920.t1 AT3G58350 30.882 136 92 1 6 139 1 136 1.04e-19 88.6
MS.gene051920.t1 AT3G58350 30.882 136 92 1 6 139 1 136 1.04e-19 88.6
MS.gene051920.t1 AT3G44800 32.168 143 97 0 11 153 3 145 1.09e-18 86.3
MS.gene051920.t1 AT3G58200 33.058 121 81 0 6 126 1 121 2.59e-18 84.7
MS.gene051920.t1 AT5G52330 34.400 125 78 2 9 133 16 136 5.38e-18 84.7
MS.gene051920.t1 AT5G52330 34.400 125 78 2 9 133 16 136 5.38e-18 84.7
MS.gene051920.t1 AT5G52330 34.400 125 78 2 9 133 16 136 5.38e-18 84.7
MS.gene051920.t1 AT5G43560 35.075 134 82 3 9 141 66 195 1.22e-17 85.1
MS.gene051920.t1 AT5G43560 35.075 134 82 3 9 141 66 195 1.22e-17 85.1
MS.gene051920.t1 AT2G05420 31.973 147 92 2 14 153 10 155 1.30e-17 82.4
MS.gene051920.t1 AT2G05410 30.872 149 101 2 6 153 1 148 3.71e-17 81.3
MS.gene051920.t1 AT1G31390 33.871 124 80 1 6 127 1 124 6.94e-17 80.1
MS.gene051920.t1 AT3G17380 32.061 131 83 3 17 141 25 155 2.59e-16 79.0
MS.gene051920.t1 AT3G17380 31.429 140 93 2 6 143 168 306 2.70e-15 75.9
MS.gene051920.t1 AT3G58220 26.286 175 121 4 14 184 13 183 3.30e-16 79.0
MS.gene051920.t1 AT1G04300 33.582 134 84 3 9 141 65 194 3.71e-16 80.5
MS.gene051920.t1 AT3G58410 32.576 132 71 2 6 137 22 135 3.90e-16 78.6
MS.gene051920.t1 AT3G44790 34.848 132 82 1 11 142 3 130 4.29e-16 78.6
MS.gene051920.t1 AT1G31400 28.947 152 103 2 6 155 1 149 4.82e-16 77.8
MS.gene051920.t1 AT2G25320 31.788 151 92 3 13 153 405 554 6.27e-16 79.7
MS.gene051920.t1 AT2G25320 27.737 137 88 3 16 141 76 212 7.15e-13 70.5
MS.gene051920.t1 AT2G25320 31.788 151 92 3 13 153 404 553 6.44e-16 79.7
MS.gene051920.t1 AT2G25320 27.737 137 88 3 16 141 75 211 7.42e-13 70.5
MS.gene051920.t1 AT2G25320 31.788 151 92 3 13 153 405 554 7.32e-16 79.7
MS.gene051920.t1 AT2G25320 27.737 137 88 3 16 141 76 212 7.43e-13 70.5
MS.gene051920.t1 AT5G26260 31.081 148 96 4 4 145 53 200 9.61e-16 77.8
MS.gene051920.t1 AT2G32870 35.507 138 81 4 13 143 277 413 7.34e-15 75.5
MS.gene051920.t1 AT3G58220 29.104 134 87 4 13 142 12 141 7.49e-15 75.9
MS.gene051920.t1 AT2G01790 29.496 139 96 1 16 152 11 149 7.63e-15 74.3
MS.gene051920.t1 AT3G58220 29.323 133 86 4 14 142 13 141 8.56e-15 75.1
MS.gene051920.t1 AT3G58290 29.252 147 93 3 12 148 10 155 9.02e-15 74.3
MS.gene051920.t1 AT3G58220 29.104 134 87 4 13 142 12 141 1.29e-14 74.7
MS.gene051920.t1 AT3G58220 29.323 133 86 4 14 142 13 141 1.34e-14 73.2
MS.gene051920.t1 AT4G09780 32.117 137 84 2 13 140 214 350 3.64e-14 73.6
MS.gene051920.t1 AT1G04300 31.690 142 84 4 9 141 65 202 8.76e-14 73.2
MS.gene051920.t1 AT2G25330 32.680 153 93 3 13 155 359 511 1.03e-13 72.8
MS.gene051920.t1 AT2G25330 26.562 128 90 3 16 139 50 177 8.03e-12 67.0
MS.gene051920.t1 AT3G58440 34.375 128 81 3 12 139 9 133 1.29e-13 72.4
MS.gene051920.t1 AT3G58240 22.393 326 225 6 6 321 1 308 1.60e-13 70.9
MS.gene051920.t1 AT4G09780 31.757 148 81 4 13 140 214 361 1.21e-12 68.9
MS.gene051920.t1 AT5G26320 30.172 116 75 2 31 140 81 196 1.69e-12 68.2
MS.gene051920.t1 AT4G09770 31.967 122 82 1 13 133 160 281 4.23e-12 66.6
MS.gene051920.t1 AT4G09770 31.967 122 82 1 13 133 160 281 4.23e-12 66.6
MS.gene051920.t1 AT2G32880 33.094 139 84 4 13 143 178 315 5.49e-12 66.2
MS.gene051920.t1 AT4G09770 31.967 122 82 1 13 133 186 307 6.52e-12 66.2
MS.gene051920.t1 AT2G42460 29.078 141 97 2 6 146 2 139 1.83e-11 64.7
MS.gene051920.t1 AT3G29580 34.188 117 73 2 13 127 8 122 6.42e-11 63.2
MS.gene051920.t1 AT3G46190 30.147 136 88 3 14 149 183 311 7.62e-11 62.8

Find 63 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGAAATAGTTTCCATATT+TGG 0.110416 7.1:-6006827 None:intergenic
GCAAACTGCCAATATGTTTA+AGG 0.125935 7.1:+6004644 MS.gene051920:CDS
CAACAGTTTATCAATATATT+TGG 0.160836 7.1:+6004614 MS.gene051920:CDS
ATAGATTTAAATTCTGAATT+AGG 0.189765 7.1:+6007331 MS.gene051920:CDS
GTGAAATAGTTTCCATATTT+GGG 0.271306 7.1:-6006826 None:intergenic
GGAATTGGAAGTCCCAAATA+TGG 0.305242 7.1:+6006814 MS.gene051920:CDS
CAGTAGTGTAGAACAATTAG+AGG 0.327754 7.1:+6006925 MS.gene051920:CDS
TTGTTTACATCGTTCCCTTT+TGG 0.328528 7.1:-6004594 None:intergenic
TCAATGCAAGTCAGAAGAAC+TGG 0.356803 7.1:+6006612 MS.gene051920:CDS
GAGCTAAACTCGTGAAAGTA+TGG 0.363455 7.1:-6006640 None:intergenic
TCAAAATGTGCATATGGAAT+TGG 0.368049 7.1:+6006799 MS.gene051920:CDS
GATTTGGCATTTGCTGCATT+AGG 0.377780 7.1:+6007004 MS.gene051920:CDS
GTGAAGGATGTTTGTATTGT+TGG 0.378900 7.1:+6006692 MS.gene051920:CDS
GCTCCATCCCTTAAACATAT+TGG 0.387375 7.1:-6004652 None:intergenic
ATTTGGCATTTGCTGCATTA+GGG 0.390923 7.1:+6007005 MS.gene051920:CDS
AGCTGAGGTTTCAGTTTGTA+AGG 0.397187 7.1:+6006715 MS.gene051920:CDS
CCTCATGTTCAATCTGCATT+AGG 0.400815 7.1:+6007148 MS.gene051920:CDS
TATGAATGACCAAGCTTGTA+AGG 0.406254 7.1:+6007066 MS.gene051920:CDS
GTAATGACGCGACTCTTCTT+CGG 0.409853 7.1:-6006980 None:intergenic
TTCACGATAAAACCGTTGTT+AGG 0.413903 7.1:-6006674 None:intergenic
CTGAATTAGGATGTGGGAGT+TGG 0.415524 7.1:+6007344 MS.gene051920:CDS
CAAACTGCCAATATGTTTAA+GGG 0.416894 7.1:+6004645 MS.gene051920:CDS
GAGTCGCGTCATTACCGATT+TGG 0.417813 7.1:+6006988 MS.gene051920:CDS
TCTGACTTGCATTGAGCTCA+TGG 0.419738 7.1:-6006604 None:intergenic
TGAAAGTCAAAATGTGCATA+TGG 0.420616 7.1:+6006793 MS.gene051920:CDS
AGGATTCTTATGTTTCCAAA+AGG 0.435968 7.1:+6004579 MS.gene051920:CDS
AGACACTTGTTGAAAGCAAA+AGG 0.441122 7.1:+6007298 MS.gene051920:CDS
CTTGAAGATCCTTACAAGCT+TGG 0.447089 7.1:-6007075 None:intergenic
TTTGGAGGTTGAAAAGTTGA+AGG 0.458143 7.1:+6007195 MS.gene051920:CDS
GTGCAAACTAGTGTAGAAAC+TGG 0.459502 7.1:-6006848 None:intergenic
TTAAATTCTGAATTAGGATG+TGG 0.474050 7.1:+6007337 MS.gene051920:CDS
TAATGCAGCAAATGCCAAAT+CGG 0.482815 7.1:-6007002 None:intergenic
AGGGTTGTTCTCACCCTCAA+TGG 0.484578 7.1:+6006886 MS.gene051920:CDS
TTATGTGGAGAAAGCTTTGG+AGG 0.493062 7.1:+6007180 MS.gene051920:CDS
AATTAGGATGTGGGAGTTGG+AGG 0.499887 7.1:+6007347 MS.gene051920:CDS
AAGTTATGTGGAGAAAGCTT+TGG 0.500157 7.1:+6007177 MS.gene051920:CDS
GGCATTTGCTGCATTAGGGA+GGG 0.501307 7.1:+6007009 MS.gene051920:CDS
GGATTCTTATGTTTCCAAAA+GGG 0.519973 7.1:+6004580 MS.gene051920:CDS
TAAATTCTGAATTAGGATGT+GGG 0.522500 7.1:+6007338 MS.gene051920:CDS
TTGTCCTCGGCGGATATCCA+TGG 0.523925 7.1:+6004421 MS.gene051920:CDS
AATTTAGATTTGATGTCACT+TGG 0.525067 7.1:+6007119 MS.gene051920:CDS
TCTTGAAACGGAGAGGCTAA+AGG 0.526186 7.1:+6007237 MS.gene051920:CDS
AATGGAGCATCAAGAAATGA+AGG 0.527699 7.1:+6004320 None:intergenic
TGCACTAAACCTACTGACAC+AGG 0.533031 7.1:+6006866 MS.gene051920:CDS
ACCTCGTGTGATTGTCATGT+TGG 0.536099 7.1:-6004718 None:intergenic
TGGCATTTGCTGCATTAGGG+AGG 0.539387 7.1:+6007008 MS.gene051920:CDS
TGTTTGTATTGTTGGAGCTG+AGG 0.551124 7.1:+6006700 MS.gene051920:CDS
CAATGCAAGTCAGAAGAACT+GGG 0.551796 7.1:+6006613 MS.gene051920:CDS
TCCGCCGAGGACAAAAGAAT+CGG 0.559266 7.1:-6004412 None:intergenic
AGATATTTGAGAAATTTACA+TGG 0.560145 7.1:+6004346 MS.gene051920:CDS
CCTAATGCAGATTGAACATG+AGG 0.581300 7.1:-6007148 None:intergenic
CTGCCAATATGTTTAAGGGA+TGG 0.581416 7.1:+6004649 MS.gene051920:CDS
TGAAAAGTTGAAGGAGAATG+TGG 0.590764 7.1:+6007204 MS.gene051920:CDS
GGCTGAGACTCAATAAGTGA+AGG 0.596886 7.1:-6006959 None:intergenic
ATTAGGAATGAAAAGTTATG+TGG 0.599520 7.1:+6007165 MS.gene051920:CDS
GGTAACGCTAGTTCTTGAAA+CGG 0.603557 7.1:+6007225 MS.gene051920:CDS
CGCTAGTTCTTGAAACGGAG+AGG 0.622684 7.1:+6007230 MS.gene051920:CDS
GAGAACAACCCTGTGTCAGT+AGG 0.632827 7.1:-6006875 None:intergenic
GCACTAAACCTACTGACACA+GGG 0.669892 7.1:+6006867 MS.gene051920:CDS
CAGCTTCATGATCCTAACAA+CGG 0.670959 7.1:+6006662 MS.gene051920:CDS
AATGCAAGTCAGAAGAACTG+GGG 0.737691 7.1:+6006614 MS.gene051920:CDS
CATACCATGGATATCCGCCG+AGG 0.754008 7.1:-6004425 None:intergenic
GCCAACATGACAATCACACG+AGG 0.760979 7.1:+6004717 MS.gene051920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATACAAAAATGTAATAAA+TGG - chr7.1:6004528-6004547 None:intergenic 10.0%
!! TTAAAATGAAAAAAAGATAA+AGG + chr7.1:6005589-6005608 MS.gene051920:intron 10.0%
!!! ATAGTTAGATAAATTTTATT+AGG - chr7.1:6005664-6005683 None:intergenic 10.0%
!!! ATCTTTTTTTCATTTTAATT+TGG - chr7.1:6005587-6005606 None:intergenic 10.0%
!!! CTTTTTTATGAAAAATTTAT+AGG + chr7.1:6004484-6004503 MS.gene051920:intron 10.0%
!!! TATATATTTTACGATTTTAT+AGG - chr7.1:6005987-6006006 None:intergenic 10.0%
!! ATAGATTTAAATTCTGAATT+AGG + chr7.1:6007331-6007350 MS.gene051920:CDS 15.0%
!! CTTAAAAATTCATATGAAAA+TGG - chr7.1:6005440-6005459 None:intergenic 15.0%
!! GTATATAAAAATTGACAAAA+AGG + chr7.1:6005560-6005579 MS.gene051920:intron 15.0%
!! TTGAAATGAAAAAAAGATAA+AGG - chr7.1:6005402-6005421 None:intergenic 15.0%
!!! TATTTTTCTAAACGATAAAA+TGG - chr7.1:6006326-6006345 None:intergenic 15.0%
!!! TTACTCTATAATTTAGAATT+TGG + chr7.1:6006139-6006158 MS.gene051920:intron 15.0%
!!! TTCATGATTTTATTTTCTTA+TGG + chr7.1:6005467-6005486 MS.gene051920:intron 15.0%
!! AAATTAAAGGACTATGATTA+AGG + chr7.1:6006400-6006419 MS.gene051920:intron 20.0%
!! ACAAAATACATTTCAAAAGA+AGG - chr7.1:6006021-6006040 None:intergenic 20.0%
!! AGATATTTGAGAAATTTACA+TGG + chr7.1:6004346-6004365 MS.gene051920:CDS 20.0%
!! CAACAGTTTATCAATATATT+TGG + chr7.1:6004614-6004633 MS.gene051920:CDS 20.0%
!! GCAACAAGATATTAATAAAA+TGG + chr7.1:6005327-6005346 MS.gene051920:intron 20.0%
!! GTTACCTAAAATAAAGTTAA+GGG - chr7.1:6005371-6005390 None:intergenic 20.0%
!! TAATATCTTGTTGCATAATT+GGG - chr7.1:6005321-6005340 None:intergenic 20.0%
!! TATAGGAGGAAAAATAATAA+AGG - chr7.1:6005970-6005989 None:intergenic 20.0%
!! TGTTACCTAAAATAAAGTTA+AGG - chr7.1:6005372-6005391 None:intergenic 20.0%
!! TTAATATCTTGTTGCATAAT+TGG - chr7.1:6005322-6005341 None:intergenic 20.0%
!! TTTAGATGAAAAATCACTAA+TGG + chr7.1:6006289-6006308 MS.gene051920:intron 20.0%
!!! AATTTTAATGAGATGTGAAT+TGG + chr7.1:6006206-6006225 MS.gene051920:intron 20.0%
!!! AGTATATATTTTCTTTTGAG+AGG + chr7.1:6005148-6005167 MS.gene051920:intron 20.0%
!!! ATACTTTATCTTTTTAACGA+CGG + chr7.1:6005934-6005953 MS.gene051920:intron 20.0%
!!! ATATTTTACGATTTTATAGG+AGG - chr7.1:6005984-6006003 None:intergenic 20.0%
!!! GAAGCTTTAGATTAAATTAA+AGG + chr7.1:6006387-6006406 MS.gene051920:intron 20.0%
!!! GTTATCTGAAATTAAGTTAA+GGG + chr7.1:6005620-6005639 MS.gene051920:intron 20.0%
!!! TGTTATCTGAAATTAAGTTA+AGG + chr7.1:6005619-6005638 MS.gene051920:intron 20.0%
!!! TTCTTTTTTAAGTAGTTTAG+TGG - chr7.1:6005096-6005115 None:intergenic 20.0%
!!! TTTTGTATTTTGATCTGATT+TGG + chr7.1:6004538-6004557 MS.gene051920:intron 20.0%
! AAATTGACATTCGTTTAAGA+TGG - chr7.1:6006262-6006281 None:intergenic 25.0%
! AATTTAGATTTGATGTCACT+TGG + chr7.1:6007119-6007138 MS.gene051920:CDS 25.0%
! ACTAAAGTGAATTACATTGT+TGG + chr7.1:6005733-6005752 MS.gene051920:intron 25.0%
! AGATATCTTAAAACGAGATA+TGG - chr7.1:6006466-6006485 None:intergenic 25.0%
! ATTAGGAATGAAAAGTTATG+TGG + chr7.1:6007165-6007184 MS.gene051920:CDS 25.0%
! CTTAATTTCAGATAACAGTT+TGG - chr7.1:6005616-6005635 None:intergenic 25.0%
! GTATAGTTGTTATCTAGAAA+AGG - chr7.1:6005918-6005937 None:intergenic 25.0%
! GTGAAATAGTTTCCATATTT+GGG - chr7.1:6006829-6006848 None:intergenic 25.0%
! TAAATTCTGAATTAGGATGT+GGG + chr7.1:6007338-6007357 MS.gene051920:CDS 25.0%
! TCATATGAAAATGGATCAAA+AGG - chr7.1:6005431-6005450 None:intergenic 25.0%
! TGAAAAATCACTAATGGTTA+TGG + chr7.1:6006295-6006314 MS.gene051920:intron 25.0%
! TTAAATTCTGAATTAGGATG+TGG + chr7.1:6007337-6007356 MS.gene051920:CDS 25.0%
! TTTCTAAACGATAAAATGGT+GGG - chr7.1:6006322-6006341 None:intergenic 25.0%
!! TTCGAATCACTTAGAAATTT+TGG + chr7.1:6004450-6004469 MS.gene051920:intron 25.0%
!! TTTTCTAAACGATAAAATGG+TGG - chr7.1:6006323-6006342 None:intergenic 25.0%
!!! ATCCATAATTTTTTCCATTG+AGG - chr7.1:6006903-6006922 None:intergenic 25.0%
!!! ATTTTGATCTGATTTGGTTT+TGG + chr7.1:6004544-6004563 MS.gene051920:intron 25.0%
!!! GTTGATTTGATTACAGTATT+AGG + chr7.1:6006521-6006540 MS.gene051920:intron 25.0%
!!! TAAGGATCTTCAAGTTTTAT+GGG + chr7.1:6007084-6007103 MS.gene051920:CDS 25.0%
!!! TAGTCCCTTAACTTTATTTT+AGG + chr7.1:6005364-6005383 MS.gene051920:intron 25.0%
!!! TATATTTTCTTTTGAGAGGT+AGG + chr7.1:6005152-6005171 MS.gene051920:intron 25.0%
!!! TCCATAATTTTTTCCATTGA+GGG - chr7.1:6006902-6006921 None:intergenic 25.0%
ACAAAATTTCTACCGTCAAA+AGG - chr7.1:6004755-6004774 None:intergenic 30.0%
ACCCTCAATGGAAAAAATTA+TGG + chr7.1:6006898-6006917 MS.gene051920:CDS 30.0%
ACTAGTATTCTACGAATGAA+AGG + chr7.1:6006173-6006192 MS.gene051920:intron 30.0%
AGGATTCTTATGTTTCCAAA+AGG + chr7.1:6004579-6004598 MS.gene051920:CDS 30.0%
ATTGTATGACATGTCATGAT+TGG - chr7.1:6004812-6004831 None:intergenic 30.0%
CAAAATTTCTACCGTCAAAA+GGG - chr7.1:6004754-6004773 None:intergenic 30.0%
CAAACTGCCAATATGTTTAA+GGG + chr7.1:6004645-6004664 MS.gene051920:CDS 30.0%
GGATTCTTATGTTTCCAAAA+GGG + chr7.1:6004580-6004599 MS.gene051920:CDS 30.0%
GGTGAAATAGTTTCCATATT+TGG - chr7.1:6006830-6006849 None:intergenic 30.0%
TAAAACGAGATATGGAAACA+TGG - chr7.1:6006458-6006477 None:intergenic 30.0%
TCAAAATGTGCATATGGAAT+TGG + chr7.1:6006799-6006818 MS.gene051920:CDS 30.0%
TGAAAGTCAAAATGTGCATA+TGG + chr7.1:6006793-6006812 MS.gene051920:CDS 30.0%
TTCTAAACGATAAAATGGTG+GGG - chr7.1:6006321-6006340 None:intergenic 30.0%
TTCTTATGCAATCATAGACA+AGG - chr7.1:6005517-6005536 None:intergenic 30.0%
TTGTGTTTCAAATATGCATG+TGG + chr7.1:6004771-6004790 MS.gene051920:intron 30.0%
! ATGATCCAATTTTCCTTTCA+AGG + chr7.1:6005257-6005276 MS.gene051920:intron 30.0%
! TCGTTTAAGATGGAATTTGT+TGG - chr7.1:6006252-6006271 None:intergenic 30.0%
! TTTGTCGTTTCATTCATTCA+TGG + chr7.1:6005202-6005221 MS.gene051920:intron 30.0%
! TTTTTCCAAGGTGAATAAGT+GGG - chr7.1:6005062-6005081 None:intergenic 30.0%
!! GTAAGGATCTTCAAGTTTTA+TGG + chr7.1:6007083-6007102 MS.gene051920:CDS 30.0%
!! TTGTTTTACACAAACGAACA+CGG + chr7.1:6004994-6005013 MS.gene051920:intron 30.0%
!!! ATCTGATTTGGTTTTGGTTT+TGG + chr7.1:6004550-6004569 MS.gene051920:intron 30.0%
!!! CATTTTGACTTTCAGATGTT+AGG - chr7.1:6006787-6006806 None:intergenic 30.0%
!!! GTTAGAATTCGTTTTTTCCA+AGG - chr7.1:6005074-6005093 None:intergenic 30.0%
!!! TTTGGTTTTGGTTTTGGTTT+AGG + chr7.1:6004556-6004575 MS.gene051920:intron 30.0%
!!! TTTTTTCCAAGGTGAATAAG+TGG - chr7.1:6005063-6005082 None:intergenic 30.0%
AAGTTATGTGGAGAAAGCTT+TGG + chr7.1:6007177-6007196 MS.gene051920:CDS 35.0%
ACGGTACATGATTATATGCA+GGG + chr7.1:6005013-6005032 MS.gene051920:intron 35.0%
AGTGATTCGAATTCATACCA+TGG - chr7.1:6004441-6004460 None:intergenic 35.0%
CAGTAGTGTAGAACAATTAG+AGG + chr7.1:6006925-6006944 MS.gene051920:CDS 35.0%
CCAATCCTTGAAAGGAAAAT+TGG - chr7.1:6005265-6005284 None:intergenic 35.0%
CTAGACTACTTGACAAAAAG+TGG + chr7.1:6005822-6005841 MS.gene051920:intron 35.0%
CTTTATCAACCAATCATGAC+AGG + chr7.1:6004936-6004955 MS.gene051920:intron 35.0%
GACATGTCATACAATGTTAG+TGG + chr7.1:6004817-6004836 MS.gene051920:intron 35.0%
GCAAACTGCCAATATGTTTA+AGG + chr7.1:6004644-6004663 MS.gene051920:CDS 35.0%
GTGAAGGATGTTTGTATTGT+TGG + chr7.1:6006692-6006711 MS.gene051920:CDS 35.0%
TAATGCAGCAAATGCCAAAT+CGG - chr7.1:6007005-6007024 None:intergenic 35.0%
TATGAATGACCAAGCTTGTA+AGG + chr7.1:6007066-6007085 MS.gene051920:CDS 35.0%
TGAAAAGTTGAAGGAGAATG+TGG + chr7.1:6007204-6007223 MS.gene051920:CDS 35.0%
TTCACGATAAAACCGTTGTT+AGG - chr7.1:6006677-6006696 None:intergenic 35.0%
TTCTAATGACCTGTCATGAT+TGG - chr7.1:6004948-6004967 None:intergenic 35.0%
TTCTACGAATGAAAGGATTC+TGG + chr7.1:6006180-6006199 MS.gene051920:intron 35.0%
TTTGGAGGTTGAAAAGTTGA+AGG + chr7.1:6007195-6007214 MS.gene051920:CDS 35.0%
! AGACACTTGTTGAAAGCAAA+AGG + chr7.1:6007298-6007317 MS.gene051920:CDS 35.0%
! ATTTGGCATTTGCTGCATTA+GGG + chr7.1:6007005-6007024 MS.gene051920:CDS 35.0%
! CCAATTTTCCTTTCAAGGAT+TGG + chr7.1:6005262-6005281 MS.gene051920:intron 35.0%
! CTTTTTAACGACGGCAAAAT+TGG + chr7.1:6005943-6005962 MS.gene051920:intron 35.0%
! TAGAACTCTATCCCTTTTGA+CGG + chr7.1:6004740-6004759 MS.gene051920:intron 35.0%
! TTGTTTACATCGTTCCCTTT+TGG - chr7.1:6004597-6004616 None:intergenic 35.0%
! TTTTCCAAGGTGAATAAGTG+GGG - chr7.1:6005061-6005080 None:intergenic 35.0%
!! TAACAACGGTTTTATCGTGA+AGG + chr7.1:6006676-6006695 MS.gene051920:CDS 35.0%
AATGCAAGTCAGAAGAACTG+GGG + chr7.1:6006614-6006633 MS.gene051920:CDS 40.0%
AGCTGAGGTTTCAGTTTGTA+AGG + chr7.1:6006715-6006734 MS.gene051920:CDS 40.0%
ATGCATTTCCCTACTACCAA+GGG - chr7.1:6005237-6005256 None:intergenic 40.0%
CAATGCAAGTCAGAAGAACT+GGG + chr7.1:6006613-6006632 MS.gene051920:CDS 40.0%
CACGGTACATGATTATATGC+AGG + chr7.1:6005012-6005031 MS.gene051920:intron 40.0%
CAGCTTCATGATCCTAACAA+CGG + chr7.1:6006662-6006681 MS.gene051920:CDS 40.0%
CCTAATGCAGATTGAACATG+AGG - chr7.1:6007151-6007170 None:intergenic 40.0%
CCTCATGTTCAATCTGCATT+AGG + chr7.1:6007148-6007167 MS.gene051920:CDS 40.0%
CGGTACATGATTATATGCAG+GGG + chr7.1:6005014-6005033 MS.gene051920:intron 40.0%
CTGCCAATATGTTTAAGGGA+TGG + chr7.1:6004649-6004668 MS.gene051920:CDS 40.0%
CTTGAAGATCCTTACAAGCT+TGG - chr7.1:6007078-6007097 None:intergenic 40.0%
GAGCTAAACTCGTGAAAGTA+TGG - chr7.1:6006643-6006662 None:intergenic 40.0%
GATGTGAATTGGACCATGAA+AGG + chr7.1:6006217-6006236 MS.gene051920:intron 40.0%
GCTCCATCCCTTAAACATAT+TGG - chr7.1:6004655-6004674 None:intergenic 40.0%
GGAATTGGAAGTCCCAAATA+TGG + chr7.1:6006814-6006833 MS.gene051920:CDS 40.0%
GTGCAAACTAGTGTAGAAAC+TGG - chr7.1:6006851-6006870 None:intergenic 40.0%
TATGCATTTCCCTACTACCA+AGG - chr7.1:6005238-6005257 None:intergenic 40.0%
TCAATGCAAGTCAGAAGAAC+TGG + chr7.1:6006612-6006631 MS.gene051920:CDS 40.0%
TGTTTGTATTGTTGGAGCTG+AGG + chr7.1:6006700-6006719 MS.gene051920:CDS 40.0%
TTGGCATGTCTTTCCTTTCA+TGG - chr7.1:6006233-6006252 None:intergenic 40.0%
TTTCCTTTCAAGGATTGGAG+TGG + chr7.1:6005267-6005286 MS.gene051920:intron 40.0%
! GATTTGGCATTTGCTGCATT+AGG + chr7.1:6007004-6007023 MS.gene051920:CDS 40.0%
! GGTAACGCTAGTTCTTGAAA+CGG + chr7.1:6007225-6007244 MS.gene051920:CDS 40.0%
! TACTCCGATTCTTTTGTCCT+CGG + chr7.1:6004408-6004427 MS.gene051920:CDS 40.0%
! TTATGTGGAGAAAGCTTTGG+AGG + chr7.1:6007180-6007199 MS.gene051920:CDS 40.0%
!! TTGAGCTCATGGTTAGTTTC+TGG - chr7.1:6006596-6006615 None:intergenic 40.0%
!!! GGTTTTGGTTTTGGTTTAGG+AGG + chr7.1:6004559-6004578 MS.gene051920:intron 40.0%
AACACCCCACTTATTCACCT+TGG + chr7.1:6005054-6005073 MS.gene051920:intron 45.0%
AATTAGGATGTGGGAGTTGG+AGG + chr7.1:6007347-6007366 MS.gene051920:CDS 45.0%
ACATGATTATATGCAGGGGC+TGG + chr7.1:6005018-6005037 MS.gene051920:intron 45.0%
ACCTCGTGTGATTGTCATGT+TGG - chr7.1:6004721-6004740 None:intergenic 45.0%
CTGAATTAGGATGTGGGAGT+TGG + chr7.1:6007344-6007363 MS.gene051920:CDS 45.0%
GCACTAAACCTACTGACACA+GGG + chr7.1:6006867-6006886 MS.gene051920:CDS 45.0%
GGCTGAGACTCAATAAGTGA+AGG - chr7.1:6006962-6006981 None:intergenic 45.0%
GTAATGACGCGACTCTTCTT+CGG - chr7.1:6006983-6007002 None:intergenic 45.0%
TCTTGAAACGGAGAGGCTAA+AGG + chr7.1:6007237-6007256 MS.gene051920:CDS 45.0%
TGCACTAAACCTACTGACAC+AGG + chr7.1:6006866-6006885 MS.gene051920:CDS 45.0%
! TCTGACTTGCATTGAGCTCA+TGG - chr7.1:6006607-6006626 None:intergenic 45.0%
!! TTGGAGTGGTGGTGGAATTT+AGG + chr7.1:6005281-6005300 MS.gene051920:intron 45.0%
!!! ATTTTAAACTTTAAAAATTA+TGG - chr7.1:6004848-6004867 None:intergenic 5.0%
AGGGTTGTTCTCACCCTCAA+TGG + chr7.1:6006886-6006905 MS.gene051920:CDS 50.0%
CCACCACTCCAATCCTTGAA+AGG - chr7.1:6005273-6005292 None:intergenic 50.0%
CCTTTCAAGGATTGGAGTGG+TGG + chr7.1:6005270-6005289 MS.gene051920:intron 50.0%
GAGAACAACCCTGTGTCAGT+AGG - chr7.1:6006878-6006897 None:intergenic 50.0%
GCCAACATGACAATCACACG+AGG + chr7.1:6004717-6004736 MS.gene051920:CDS 50.0%
TCCGCCGAGGACAAAAGAAT+CGG - chr7.1:6004415-6004434 None:intergenic 50.0%
TTCAAGGATTGGAGTGGTGG+TGG + chr7.1:6005273-6005292 MS.gene051920:intron 50.0%
TTCATGGACTCTCTGACCCT+TGG + chr7.1:6005218-6005237 MS.gene051920:intron 50.0%
! ACTCTCTGACCCTTGGTAGT+AGG + chr7.1:6005225-6005244 MS.gene051920:intron 50.0%
! CGCTAGTTCTTGAAACGGAG+AGG + chr7.1:6007230-6007249 MS.gene051920:CDS 50.0%
! CTCTCTGACCCTTGGTAGTA+GGG + chr7.1:6005226-6005245 MS.gene051920:intron 50.0%
! GAGTCGCGTCATTACCGATT+TGG + chr7.1:6006988-6007007 MS.gene051920:CDS 50.0%
! GGCATTTGCTGCATTAGGGA+GGG + chr7.1:6007009-6007028 MS.gene051920:CDS 50.0%
! GGTGAATAAGTGGGGTGTTC+CGG - chr7.1:6005053-6005072 None:intergenic 50.0%
! TCCGATTCTTTTGTCCTCGG+CGG + chr7.1:6004411-6004430 MS.gene051920:CDS 50.0%
! TGGCATTTGCTGCATTAGGG+AGG + chr7.1:6007008-6007027 MS.gene051920:CDS 50.0%
CATACCATGGATATCCGCCG+AGG - chr7.1:6004428-6004447 None:intergenic 55.0%
TTGTCCTCGGCGGATATCCA+TGG + chr7.1:6004421-6004440 MS.gene051920:CDS 55.0%
CAGGGGCTGGAGTTCGAAGC+CGG + chr7.1:6005031-6005050 MS.gene051920:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 6004321 6007375 6004321 ID=MS.gene051920
chr7.1 mRNA 6004321 6007375 6004321 ID=MS.gene051920.t1;Parent=MS.gene051920
chr7.1 exon 6004321 6004442 6004321 ID=MS.gene051920.t1.exon1;Parent=MS.gene051920.t1
chr7.1 CDS 6004321 6004442 6004321 ID=cds.MS.gene051920.t1;Parent=MS.gene051920.t1
chr7.1 exon 6004578 6004738 6004578 ID=MS.gene051920.t1.exon2;Parent=MS.gene051920.t1
chr7.1 CDS 6004578 6004738 6004578 ID=cds.MS.gene051920.t1;Parent=MS.gene051920.t1
chr7.1 exon 6006597 6007375 6006597 ID=MS.gene051920.t1.exon3;Parent=MS.gene051920.t1
chr7.1 CDS 6006597 6007375 6006597 ID=cds.MS.gene051920.t1;Parent=MS.gene051920.t1
Gene Sequence

>MS.gene051920

ATGGAGCATCAAGAAATGAAGGATGAGATATTTGAGAAATTTACATGGAAGATTGAGAACTTCTCTCGTTTGAACACTAATGAGCTTTACTCCGATTCTTTTGTCCTCGGCGGATATCCATGGTATGAATTCGAATCACTTAGAAATTTTGGATATATATTTTCTTTTTTATGAAAAATTTATAGGATATGTTGATCTTTGATACCATTTATTACATTTTTGTATTTTGATCTGATTTGGTTTTGGTTTTGGTTTAGGAGGATTCTTATGTTTCCAAAAGGGAACGATGTAAACAACAGTTTATCAATATATTTGGAAGCTATGCAAACTGCCAATATGTTTAAGGGATGGAGCAGAGATGTGAAATTTAAACTGCTTGTATTCAATCAGATCAATGCCAACATGACAATCACACGAGGTAGAACTCTATCCCTTTTGACGGTAGAAATTTTGTGTTTCAAATATGCATGTGGTATATGCAAATGAAACCAATCATGACATGTCATACAATGTTAGTGGTACATCCATAATTTTTAAAGTTTAAAATAATATTTTTTTTTTGAAGAATATAATAATTTAATTTATATACAATGTTAGTGTGAAGAGTTTTTTTTACTTTATCAACCAATCATGACAGGTCATTAGAAACATTTTGATGTGCTGTCAATTTAAATTGTTTTACACAAACGAACACGGTACATGATTATATGCAGGGGCTGGAGTTCGAAGCCGGAACACCCCACTTATTCACCTTGGAAAAAACGAATTCTAACCACTAAACTACTTAAAAAAGAAAAAAAAAAAACGATAACTAAAAAAACTACGAAAGTATATATTTTCTTTTGAGAGGTAGGAAAGTATTATTTGCTTCTTTATTTTATTTTGTCGTTTCATTCATTCATGGACTCTCTGACCCTTGGTAGTAGGGAAATGCATATGATCCAATTTTCCTTTCAAGGATTGGAGTGGTGGTGGAATTTAGGCTCACTTGACACTACCCAATTATGCAACAAGATATTAATAAAATGGCAAAATTACACTTTTAGTCCCTTAACTTTATTTTAGGTAACAGTTTAGTCCTTTATCTTTTTTTCATTTCAATTTTGTCCTTTTGATCCATTTTCATATGAATTTTTAAGCTTAAAATTCATGATTTTATTTTCTTATGGTCTTTCAGTCTTCAAAATTATGATCCTTGTCTATGATTGCATAAGAATATTAAATTTGAAGTTTGAAAATGTATATAAAAATTGACAAAAAGGACCAAATTAAAATGAAAAAAAGATAAAGGACCAAACTGTTATCTGAAATTAAGTTAAGGGACTAAAAATGTAATTTTGCCTAATAAAATTTATCTAACTATATATCGTTTTTGAAATTTACTCTTGTTTCTCTGAATTTGTGTGTTATTTACTAAAGTGAATTACATTGTTGGACAAAGAGATTTATAAATTTAATATTAGTATTGTCGCTAAAATTGTAGAAGAGATAATTCTAACTACTAGACTACTTGACAAAAAGTGGTAATATTATATAATCTAGACAAAACTTTTCTAGTGATCTTTTAACTTAATTTACAATTTTAAATCGATTTCCTTTTCTAGATAACAACTATACTTTATCTTTTTAACGACGGCAAAATTGGACCTTTATTATTTTTCCTCCTATAAAATCGTAAAATATATAAATTATATTGACCTTCTTTTGAAATGTATTTTGTCATAATGTTTTCATTTTTTAATTATACTGTCATTTTTACACTATAATTTAGAATGTATTTTGTCATAATGTTTTTATTTTTTAATTAAATTGTCATTTTTACTCTATAATTTAGAATTTGGATGTCATTTTGACTAGTATTCTACGAATGAAAGGATTCTGGTTTAATTTTAATGAGATGTGAATTGGACCATGAAAGGAAAGACATGCCAACAAATTCCATCTTAAACGAATGTCAATTTTCATGTTTTTAGATGAAAAATCACTAATGGTTATGGCCCCACCATTTTATCGTTTAGAAAAATAAATGACATTTTTATCTAAAAAAACAAATAAATATTTTGTCTGAAGCTTTAGATTAAATTAAAGGACTATGATTAAGGTTTGCATCTAATCTATCACTGCTCTCATGCAACCATGTTTCCATATCTCGTTTTAAGATATCTTTTATGACTATCTTACTTAAAAATTGCTTTCATGTGTTGATTTGATTACAGTATTAGGCATAAATTCTTTTTTATGTAACTAAATTGATTCTTCATACATCAATTTACCAGAAACTAACCATGAGCTCAATGCAAGTCAGAAGAACTGGGGTTTCCATACTTTCACGAGTTTAGCTCAGCTTCATGATCCTAACAACGGTTTTATCGTGAAGGATGTTTGTATTGTTGGAGCTGAGGTTTCAGTTTGTAAGGTAACACATGAGAACAAAGTGAATCAAGAAGCGAACTTAGCTGTTTTCCTAACATCTGAAAGTCAAAATGTGCATATGGAATTGGAAGTCCCAAATATGGAAACTATTTCACCAGTTTCTACACTAGTTTGCACTAAACCTACTGACACAGGGTTGTTCTCACCCTCAATGGAAAAAATTATGGATTTCAGTAGTGTAGAACAATTAGAGGAAATATATAATCCTTCACTTATTGAGTCTCAGCCGAAGAAGAGTCGCGTCATTACCGATTTGGCATTTGCTGCATTAGGGAGGGTTTTTTATTTCTTTAAGACTAAAAAAGTGAAAGATATGAATGACCAAGCTTGTAAGGATCTTCAAGTTTTATGGGATGAACTTAAGAAATTTAGATTTGATGTCACTTGGCTAGAGCCTCATGTTCAATCTGCATTAGGAATGAAAAGTTATGTGGAGAAAGCTTTGGAGGTTGAAAAGTTGAAGGAGAATGTGGTAACGCTAGTTCTTGAAACGGAGAGGCTAAAGGCAAAGTTGCTTGCTGCTGAAGTAAATCTTGACATAGAAAGACACTTGTTGAAAGCAAAAGGCTTCAAAGAAATAGATTTAAATTCTGAATTAGGATGTGGGAGTTGGAGGCCATAG
Protein sequence

>MS.gene051920.t1

MEHQEMKDEIFEKFTWKIENFSRLNTNELYSDSFVLGGYPWRILMFPKGNDVNNSLSIYLEAMQTANMFKGWSRDVKFKLLVFNQINANMTITRETNHELNASQKNWGFHTFTSLAQLHDPNNGFIVKDVCIVGAEVSVCKVTHENKVNQEANLAVFLTSESQNVHMELEVPNMETISPVSTLVCTKPTDTGLFSPSMEKIMDFSSVEQLEEIYNPSLIESQPKKSRVITDLAFAALGRVFYFFKTKKVKDMNDQACKDLQVLWDELKKFRFDVTWLEPHVQSALGMKSYVEKALEVEKLKENVVTLVLETERLKAKLLAAEVNLDIERHLLKAKGFKEIDLNSELGCGSWRP