AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063219

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063219.t1 MTR_1g042160 80.805 323 47 2 1 323 1 308 0.0 526
MS.gene063219.t1 MTR_7g107200 42.408 382 157 5 1 325 1 376 3.34e-102 305
MS.gene063219.t1 MTR_7g107160 43.582 335 166 7 2 323 119 443 1.07e-77 244
MS.gene063219.t1 MTR_7g107160 57.018 114 47 1 69 182 1 112 1.12e-36 137
MS.gene063219.t1 MTR_7g107170 59.006 161 52 2 183 329 109 269 7.35e-55 180
MS.gene063219.t1 MTR_7g107170 54.023 87 40 0 228 314 1 87 7.67e-23 96.3
MS.gene063219.t1 MTR_8g011730 31.180 356 191 7 12 326 13 355 2.40e-46 160
MS.gene063219.t1 MTR_3g079840 46.923 130 67 1 13 142 57 184 1.59e-37 142
MS.gene063219.t1 MTR_3g079840 46.923 130 67 1 13 142 56 183 1.62e-37 142
MS.gene063219.t1 MTR_5g086700 45.385 130 69 1 13 142 61 188 1.19e-36 140
MS.gene063219.t1 MTR_5g086700 45.385 130 69 1 13 142 61 188 1.20e-36 140
MS.gene063219.t1 MTR_5g091450 41.270 126 73 1 13 138 33 157 1.45e-31 125
MS.gene063219.t1 MTR_1g041240 42.748 131 72 2 13 143 58 185 2.19e-31 125
MS.gene063219.t1 MTR_1g041240 42.748 131 72 2 13 143 59 186 2.21e-31 125
MS.gene063219.t1 MTR_1g041240 42.748 131 72 2 13 143 57 184 2.48e-31 124
MS.gene063219.t1 MTR_5g091180 39.416 137 80 2 13 149 46 179 8.23e-27 111
MS.gene063219.t1 MTR_5g091550 35.433 127 80 1 12 138 18 142 5.25e-23 99.8
MS.gene063219.t1 MTR_5g091550 35.433 127 80 1 12 138 18 142 5.32e-23 99.4
MS.gene063219.t1 MTR_3g106700 31.915 141 87 2 13 144 406 546 3.97e-19 89.0
MS.gene063219.t1 MTR_3g106700 28.906 128 88 3 16 140 71 198 3.67e-13 70.9
MS.gene063219.t1 MTR_4g123750 23.718 156 111 3 14 161 20 175 7.75e-15 74.3
MS.gene063219.t1 MTR_4g123750 31.008 129 87 2 13 140 176 303 1.11e-11 65.1
MS.gene063219.t1 MTR_1g073800 27.891 147 102 4 2 147 38 181 4.18e-14 73.2
MS.gene063219.t1 MTR_1g073790 27.891 147 100 4 2 147 51 192 1.28e-13 72.4
MS.gene063219.t1 MTR_1g044450 29.630 135 89 4 9 142 61 190 5.58e-13 70.5
MS.gene063219.t1 MTR_5g091530 36.047 86 55 0 54 139 1 86 3.54e-12 67.0
MS.gene063219.t1 MTR_2g034780 34.586 133 70 5 195 323 140 259 1.09e-11 64.7
MS.gene063219.t1 MTR_8g006190 26.667 120 83 2 26 140 5 124 3.27e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063219.t1 AT5G06600 50.000 130 63 1 13 142 55 182 5.55e-37 141
MS.gene063219.t1 AT5G06600 50.000 130 63 1 13 142 56 183 5.55e-37 141
MS.gene063219.t1 AT5G06600 50.000 130 63 1 13 142 56 183 5.98e-37 141
MS.gene063219.t1 AT3G11910 49.231 130 64 1 13 142 54 181 1.15e-36 140
MS.gene063219.t1 AT3G11910 49.231 130 64 1 13 142 54 181 1.15e-36 140
MS.gene063219.t1 AT3G11910 49.231 130 64 1 13 142 55 182 1.17e-36 140
MS.gene063219.t1 AT3G11910 49.231 130 64 1 13 142 55 182 1.17e-36 140
MS.gene063219.t1 AT3G58250 37.984 129 80 0 12 140 9 137 1.14e-26 107
MS.gene063219.t1 AT3G58270 38.636 132 79 1 9 140 4 133 6.57e-25 103
MS.gene063219.t1 AT3G58270 38.636 132 79 1 9 140 4 133 6.57e-25 103
MS.gene063219.t1 AT3G58270 38.636 132 79 1 9 140 4 133 6.57e-25 103
MS.gene063219.t1 AT3G58360 26.557 305 196 6 9 301 4 292 3.44e-21 92.4
MS.gene063219.t1 AT3G58340 33.071 127 83 1 12 138 7 131 4.71e-21 92.0
MS.gene063219.t1 AT3G58340 33.071 127 83 1 12 138 7 131 5.83e-21 92.0
MS.gene063219.t1 AT2G05410 36.000 125 77 2 5 129 2 123 5.94e-19 86.3
MS.gene063219.t1 AT3G58210 32.812 128 86 0 13 140 8 135 4.50e-18 84.0
MS.gene063219.t1 AT3G58370 34.328 134 85 2 7 138 3 135 1.97e-17 81.6
MS.gene063219.t1 AT3G58410 30.769 130 85 2 9 138 4 128 7.62e-17 80.5
MS.gene063219.t1 AT3G58350 32.090 134 88 2 12 143 7 139 9.21e-17 79.7
MS.gene063219.t1 AT3G58350 32.090 134 88 2 12 143 7 139 9.21e-17 79.7
MS.gene063219.t1 AT2G25320 30.496 141 88 3 13 144 405 544 1.08e-16 81.6
MS.gene063219.t1 AT2G25320 29.688 128 87 3 16 140 76 203 1.23e-13 72.4
MS.gene063219.t1 AT2G25320 30.496 141 88 3 13 144 404 543 1.09e-16 81.6
MS.gene063219.t1 AT2G25320 29.688 128 87 3 16 140 75 202 1.23e-13 72.4
MS.gene063219.t1 AT2G25320 30.496 141 88 3 13 144 405 544 2.07e-16 80.9
MS.gene063219.t1 AT2G25320 29.688 128 87 3 16 140 76 203 1.22e-13 72.4
MS.gene063219.t1 AT2G25330 31.915 141 87 2 13 144 359 499 3.90e-16 79.7
MS.gene063219.t1 AT2G25330 28.906 128 88 3 16 140 50 177 1.37e-12 68.9
MS.gene063219.t1 AT3G27040 29.630 135 94 1 12 146 8 141 8.60e-16 76.6
MS.gene063219.t1 AT3G58290 27.941 136 88 3 12 138 10 144 5.73e-15 74.3
MS.gene063219.t1 AT2G01790 30.952 126 86 1 16 140 11 136 7.80e-15 73.9
MS.gene063219.t1 AT3G17380 28.467 137 93 2 17 148 25 161 8.93e-15 74.3
MS.gene063219.t1 AT3G17380 29.457 129 89 2 13 140 175 302 2.27e-12 67.0
MS.gene063219.t1 AT3G58260 29.851 134 92 2 9 140 4 137 1.21e-14 73.9
MS.gene063219.t1 AT3G44800 31.538 130 88 1 11 140 3 131 1.35e-14 73.9
MS.gene063219.t1 AT3G58200 32.759 116 75 2 13 127 8 121 1.84e-14 73.6
MS.gene063219.t1 AT2G05420 30.075 133 84 3 15 139 11 142 2.36e-13 70.1
MS.gene063219.t1 AT3G58410 28.462 130 74 2 9 138 25 135 6.61e-13 68.9
MS.gene063219.t1 AT5G26260 26.752 157 106 4 12 159 58 214 8.54e-13 68.9
MS.gene063219.t1 AT5G52330 28.662 157 100 5 9 165 16 160 1.16e-12 68.6
MS.gene063219.t1 AT5G52330 28.662 157 100 5 9 165 16 160 1.16e-12 68.6
MS.gene063219.t1 AT5G52330 28.662 157 100 5 9 165 16 160 1.16e-12 68.6
MS.gene063219.t1 AT5G43560 30.952 126 82 3 9 134 66 186 1.90e-12 68.6
MS.gene063219.t1 AT5G43560 30.952 126 82 3 9 134 66 186 1.90e-12 68.6
MS.gene063219.t1 AT4G09780 36.290 124 75 4 13 134 214 335 4.03e-12 67.0
MS.gene063219.t1 AT5G26320 28.889 135 90 3 31 159 81 215 1.01e-11 65.5
MS.gene063219.t1 AT2G42480 33.333 129 77 5 14 138 8 131 2.64e-11 64.3
MS.gene063219.t1 AT1G31390 27.731 119 85 1 12 129 7 125 7.63e-11 62.4
MS.gene063219.t1 AT2G42460 32.374 139 90 3 8 146 4 138 9.48e-11 62.4

Find 74 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAAAGAGTTTCAATATT+TGG 0.072812 1.3:-25376486 None:intergenic
TTGCATGTTCATTATGCTTT+AGG 0.157905 1.3:+25376699 MS.gene063219:CDS
CGACCAGTTACATATATATT+TGG 0.208741 1.3:+25375888 MS.gene063219:CDS
TGCATGTTCATTATGCTTTA+GGG 0.269895 1.3:+25376700 MS.gene063219:CDS
GTAAAAGAGTTTCAATATTT+GGG 0.278091 1.3:-25376485 None:intergenic
GAAGGAGAGCATGGTAGTTC+TGG 0.285409 1.3:+25376764 MS.gene063219:CDS
TAAAACTTGAAGATCCTTAC+AGG 0.295125 1.3:-25376631 None:intergenic
GACCTTCAGGCTTTGCTGTT+AGG 0.321081 1.3:-25376458 None:intergenic
TCCGAGCCTTTCGTCCTTAG+TGG 0.329886 1.3:+25375701 MS.gene063219:CDS
ATATTTGGGCCTCGACCTTC+AGG 0.334872 1.3:-25376471 None:intergenic
GTAGATTTAGATTCTGAATT+GGG 0.350592 1.3:+25376882 MS.gene063219:CDS
GGCTTGCTCGTTCATGTCTC+TGG 0.355614 1.3:-25376610 None:intergenic
CTGCACTACTCTTCCCCTTT+GGG 0.355811 1.3:-25375864 None:intergenic
CTTACATTTGGTAGTCAAAC+TGG 0.355935 1.3:+25376411 MS.gene063219:CDS
AGTAGATTTAGATTCTGAAT+TGG 0.356087 1.3:+25376881 MS.gene063219:CDS
CAGGCTTTGCTGTTAGGACT+AGG 0.365049 1.3:-25376452 None:intergenic
GTGAATGATACTTGTATTGT+TGG 0.377087 1.3:+25376318 MS.gene063219:CDS
ACTGCACTACTCTTCCCCTT+TGG 0.377114 1.3:-25375865 None:intergenic
AATTTAAATATGATATTACT+TGG 0.390669 1.3:+25376670 MS.gene063219:CDS
ATCCCTTCATCCCTTACATT+TGG 0.392215 1.3:+25376399 MS.gene063219:CDS
CCTCCAAATATATATGTAAC+TGG 0.394155 1.3:-25375891 None:intergenic
GAGTCGGTGTTTGCTGCATT+AGG 0.394888 1.3:+25376555 MS.gene063219:CDS
AGAGACTTGTTGAAATCAAA+AGG 0.412312 1.3:+25376849 MS.gene063219:CDS
GGTAGTTCTGGAGCTCGAAT+TGG 0.424459 1.3:+25376776 MS.gene063219:CDS
CTACCAAATGTAAGGGATGA+AGG 0.432592 1.3:-25376402 None:intergenic
TCAGTGCAAGTCAGGATGAC+TGG 0.435022 1.3:+25376238 MS.gene063219:CDS
AAGATATGTGGAGAAAGCTT+TGG 0.447649 1.3:+25376728 MS.gene063219:CDS
CATGAACGAGCAAGCCTGTA+AGG 0.453721 1.3:+25376617 MS.gene063219:CDS
CCACGAGTTCAGTGCAAGTC+AGG 0.455144 1.3:+25376230 MS.gene063219:CDS
TGGTAGTCAAACTGGTCAAA+TGG 0.456911 1.3:+25376419 MS.gene063219:CDS
CAGTTTGACTACCAAATGTA+AGG 0.457602 1.3:-25376410 None:intergenic
TTAGTTGAGCTTTGTCATCC+TGG 0.458249 1.3:+25376282 MS.gene063219:CDS
TTGAGCTTTGTCATCCTGGA+AGG 0.471871 1.3:+25376286 MS.gene063219:CDS
AGGATTCTTCTCTTCCCAAA+GGG 0.475030 1.3:+25375850 MS.gene063219:CDS
GAGCTTTGTCATCCTGGAAG+GGG 0.486742 1.3:+25376288 MS.gene063219:CDS
GCAAACACCGACTCTGCGTC+AGG 0.489005 1.3:-25376546 None:intergenic
TGAGCTTTGTCATCCTGGAA+GGG 0.489024 1.3:+25376287 MS.gene063219:CDS
TTAGATTCTGAATTGGGATG+TGG 0.494944 1.3:+25376888 MS.gene063219:CDS
TAGATTCTGAATTGGGATGT+GGG 0.496207 1.3:+25376889 MS.gene063219:CDS
TTTGGAGGCTGAAAAGTTGA+AGG 0.501303 1.3:+25376746 MS.gene063219:CDS
CAGTGCAAGTCAGGATGACT+GGG 0.502643 1.3:+25376239 MS.gene063219:CDS
GAATTGGGATGTGGGAGTTG+TGG 0.508997 1.3:+25376897 MS.gene063219:CDS
GACAACAGAACAATCACAAA+AGG 0.511099 1.3:+25375991 MS.gene063219:CDS
CCAGTTACATATATATTTGG+AGG 0.512397 1.3:+25375891 MS.gene063219:CDS
ATATGTGGAGAAAGCTTTGG+AGG 0.523862 1.3:+25376731 MS.gene063219:CDS
TCAAACTGGTCAAATGGAAG+TGG 0.534780 1.3:+25376425 MS.gene063219:CDS
GCTCGAATTGGAAAGGCTAA+AGG 0.542142 1.3:+25376788 MS.gene063219:CDS
AGCTTTGTCATCCTGGAAGG+GGG 0.555993 1.3:+25376289 MS.gene063219:CDS
TACCAAATGTAAGGGATGAA+GGG 0.561982 1.3:-25376401 None:intergenic
GTCCTAACAGCAAAGCCTGA+AGG 0.568118 1.3:+25376456 MS.gene063219:CDS
CTTACCATGGATATCCACTA+AGG 0.579500 1.3:-25375715 None:intergenic
GAAGACTGCTAATATGTCTG+AGG 0.579958 1.3:+25375918 MS.gene063219:CDS
TGAAAAGTTGAAGGAGAGCA+TGG 0.580493 1.3:+25376755 MS.gene063219:CDS
CCTGACTTGCACTGAACTCG+TGG 0.580933 1.3:-25376230 None:intergenic
TGATACTTGTATTGTTGGTG+TGG 0.581139 1.3:+25376323 MS.gene063219:CDS
ACAGCAAAGCCTGAAGGTCG+AGG 0.581162 1.3:+25376462 MS.gene063219:CDS
TCCACTAAGGACGAAAGGCT+CGG 0.589489 1.3:-25375702 None:intergenic
CTGCTAATATGTCTGAGGGA+TGG 0.590956 1.3:+25375923 MS.gene063219:CDS
GAGGATTCTTCTCTTCCCAA+AGG 0.594512 1.3:+25375849 MS.gene063219:CDS
TTCTGGAGCTCGAATTGGAA+AGG 0.594903 1.3:+25376781 MS.gene063219:CDS
TGCTGTATTCAATCAAGTCG+AGG 0.599719 1.3:+25375969 MS.gene063219:CDS
TCGTCCTTAGTGGATATCCA+TGG 0.600369 1.3:+25375711 MS.gene063219:CDS
TAAAGATCCTGACGCAGAGT+CGG 0.611168 1.3:+25376539 MS.gene063219:CDS
TAACTTATCAACATTCAAGT+GGG 0.611555 1.3:-25375675 None:intergenic
GGATATCCACTAAGGACGAA+AGG 0.618904 1.3:-25375707 None:intergenic
CTCGAGTTTACAGAAATGGA+GGG 0.619993 1.3:+25375596 None:intergenic
AAGACTGCTAATATGTCTGA+GGG 0.621113 1.3:+25375919 MS.gene063219:CDS
TTCTTCACTAGTTTCTACTG+AGG 0.622627 1.3:+25376512 MS.gene063219:CDS
AGTTTGACTACCAAATGTAA+GGG 0.630632 1.3:-25376409 None:intergenic
ATAACTTATCAACATTCAAG+TGG 0.634087 1.3:-25375676 None:intergenic
GGATTCTTCTCTTCCCAAAG+GGG 0.641187 1.3:+25375851 MS.gene063219:CDS
GGAAAGGCTAAAGGTCAAGT+CGG 0.643411 1.3:+25376797 MS.gene063219:CDS
AGATATTTGAGAAGTTCACA+TGG 0.666468 1.3:+25375636 MS.gene063219:CDS
AGTGCAAGTCAGGATGACTG+GGG 0.764553 1.3:+25376240 MS.gene063219:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAAATATGATATTACT+TGG + chr1.3:25376670-25376689 MS.gene063219:CDS 10.0%
!! GTAAAAGAGTTTCAATATTT+GGG - chr1.3:25376488-25376507 None:intergenic 20.0%
!! TGTAAAAGAGTTTCAATATT+TGG - chr1.3:25376489-25376508 None:intergenic 20.0%
!!! ATTTGTGTTTTGATCTTATT+TGG + chr1.3:25375815-25375834 MS.gene063219:intron 20.0%
! AGTAGATTTAGATTCTGAAT+TGG + chr1.3:25376881-25376900 MS.gene063219:CDS 25.0%
! ATAACTTATCAACATTCAAG+TGG - chr1.3:25375679-25375698 None:intergenic 25.0%
! GTAGATTTAGATTCTGAATT+GGG + chr1.3:25376882-25376901 MS.gene063219:CDS 25.0%
! TAACTTATCAACATTCAAGT+GGG - chr1.3:25375678-25375697 None:intergenic 25.0%
! TAACTTCTGCAAAATTATAG+TGG - chr1.3:25376102-25376121 None:intergenic 25.0%
! TTCATTCAAATAACTTACCA+TGG - chr1.3:25375731-25375750 None:intergenic 25.0%
! TTTAGGGATAAAAAGATATG+TGG + chr1.3:25376716-25376735 MS.gene063219:CDS 25.0%
!!! ATCTTATTTGGTTTTGTTGT+AGG + chr1.3:25375827-25375846 MS.gene063219:intron 25.0%
!!! TAAGGATCTTCAAGTTTTAT+GGG + chr1.3:25376635-25376654 MS.gene063219:CDS 25.0%
AGATATTTGAGAAGTTCACA+TGG + chr1.3:25375636-25375655 MS.gene063219:CDS 30.0%
AGTTTGACTACCAAATGTAA+GGG - chr1.3:25376412-25376431 None:intergenic 30.0%
CCAGTTACATATATATTTGG+AGG + chr1.3:25375891-25375910 MS.gene063219:CDS 30.0%
CCTCCAAATATATATGTAAC+TGG - chr1.3:25375894-25375913 None:intergenic 30.0%
CGACCAGTTACATATATATT+TGG + chr1.3:25375888-25375907 MS.gene063219:CDS 30.0%
GTGAATGATACTTGTATTGT+TGG + chr1.3:25376318-25376337 MS.gene063219:CDS 30.0%
TAAAACTTGAAGATCCTTAC+AGG - chr1.3:25376634-25376653 None:intergenic 30.0%
TGCATGTTCATTATGCTTTA+GGG + chr1.3:25376700-25376719 MS.gene063219:CDS 30.0%
TTAATCTATATATCCACCAG+TGG - chr1.3:25375763-25375782 None:intergenic 30.0%
TTGCATGTTCATTATGCTTT+AGG + chr1.3:25376699-25376718 MS.gene063219:CDS 30.0%
! GTGTATGTTTGATTTCATTG+TGG + chr1.3:25376053-25376072 MS.gene063219:intron 30.0%
!! AGAGACTTGTTGAAATCAAA+AGG + chr1.3:25376849-25376868 MS.gene063219:CDS 30.0%
!! GTAAGGATCTTCAAGTTTTA+TGG + chr1.3:25376634-25376653 MS.gene063219:CDS 30.0%
!!! TTATTTGGTTTTGTTGTAGG+AGG + chr1.3:25375830-25375849 MS.gene063219:intron 30.0%
AAGACTGCTAATATGTCTGA+GGG + chr1.3:25375919-25375938 MS.gene063219:CDS 35.0%
AAGATATGTGGAGAAAGCTT+TGG + chr1.3:25376728-25376747 MS.gene063219:CDS 35.0%
CACTTAATCACTTTGCAAGA+CGG - chr1.3:25376036-25376055 None:intergenic 35.0%
CAGTTTGACTACCAAATGTA+AGG - chr1.3:25376413-25376432 None:intergenic 35.0%
GAATGAATCACAATTTCCAC+TGG + chr1.3:25375744-25375763 MS.gene063219:intron 35.0%
GACAACAGAACAATCACAAA+AGG + chr1.3:25375991-25376010 MS.gene063219:CDS 35.0%
TACCAAATGTAAGGGATGAA+GGG - chr1.3:25376404-25376423 None:intergenic 35.0%
TAGATTCTGAATTGGGATGT+GGG + chr1.3:25376889-25376908 MS.gene063219:CDS 35.0%
TGATACTTGTATTGTTGGTG+TGG + chr1.3:25376323-25376342 MS.gene063219:CDS 35.0%
TTAGATTCTGAATTGGGATG+TGG + chr1.3:25376888-25376907 MS.gene063219:CDS 35.0%
! CTTACATTTGGTAGTCAAAC+TGG + chr1.3:25376411-25376430 MS.gene063219:CDS 35.0%
! TTCTTCACTAGTTTCTACTG+AGG + chr1.3:25376512-25376531 MS.gene063219:CDS 35.0%
AGGATTCTTCTCTTCCCAAA+GGG + chr1.3:25375850-25375869 MS.gene063219:CDS 40.0%
ATCCCTTCATCCCTTACATT+TGG + chr1.3:25376399-25376418 MS.gene063219:CDS 40.0%
CTACCAAATGTAAGGGATGA+AGG - chr1.3:25376405-25376424 None:intergenic 40.0%
CTTACCATGGATATCCACTA+AGG - chr1.3:25375718-25375737 None:intergenic 40.0%
GAAGACTGCTAATATGTCTG+AGG + chr1.3:25375918-25375937 MS.gene063219:CDS 40.0%
TCAAACTGGTCAAATGGAAG+TGG + chr1.3:25376425-25376444 MS.gene063219:CDS 40.0%
TGAAAAGTTGAAGGAGAGCA+TGG + chr1.3:25376755-25376774 MS.gene063219:CDS 40.0%
TGAATCACAATTTCCACTGG+TGG + chr1.3:25375747-25375766 MS.gene063219:intron 40.0%
TGCTGTATTCAATCAAGTCG+AGG + chr1.3:25375969-25375988 MS.gene063219:CDS 40.0%
TGGTAGTCAAACTGGTCAAA+TGG + chr1.3:25376419-25376438 MS.gene063219:CDS 40.0%
TTAATCACTTTGCAAGACGG+AGG - chr1.3:25376033-25376052 None:intergenic 40.0%
TTTGGAGGCTGAAAAGTTGA+AGG + chr1.3:25376746-25376765 MS.gene063219:CDS 40.0%
! ATATGTGGAGAAAGCTTTGG+AGG + chr1.3:25376731-25376750 MS.gene063219:CDS 40.0%
! TTAGTTGAGCTTTGTCATCC+TGG + chr1.3:25376282-25376301 MS.gene063219:CDS 40.0%
!! GGATCTTCAAGTTTTATGGG+AGG + chr1.3:25376638-25376657 MS.gene063219:CDS 40.0%
!!! CTCTGGCTTTTCTAGTCTTA+AGG - chr1.3:25376596-25376615 None:intergenic 40.0%
CTGCTAATATGTCTGAGGGA+TGG + chr1.3:25375923-25375942 MS.gene063219:CDS 45.0%
GAGGATTCTTCTCTTCCCAA+AGG + chr1.3:25375849-25375868 MS.gene063219:CDS 45.0%
GCTCGAATTGGAAAGGCTAA+AGG + chr1.3:25376788-25376807 MS.gene063219:CDS 45.0%
GGAAAGGCTAAAGGTCAAGT+CGG + chr1.3:25376797-25376816 MS.gene063219:CDS 45.0%
GGATATCCACTAAGGACGAA+AGG - chr1.3:25375710-25375729 None:intergenic 45.0%
GGATTCTTCTCTTCCCAAAG+GGG + chr1.3:25375851-25375870 MS.gene063219:CDS 45.0%
TAAAGATCCTGACGCAGAGT+CGG + chr1.3:25376539-25376558 MS.gene063219:CDS 45.0%
TTCACAATAAACCCCCTTCC+AGG - chr1.3:25376303-25376322 None:intergenic 45.0%
! TCGTCCTTAGTGGATATCCA+TGG + chr1.3:25375711-25375730 MS.gene063219:CDS 45.0%
! TGAGCTTTGTCATCCTGGAA+GGG + chr1.3:25376287-25376306 MS.gene063219:CDS 45.0%
! TTGAGCTTTGTCATCCTGGA+AGG + chr1.3:25376286-25376305 MS.gene063219:CDS 45.0%
!! TTCTGGAGCTCGAATTGGAA+AGG + chr1.3:25376781-25376800 MS.gene063219:CDS 45.0%
ACTGCACTACTCTTCCCCTT+TGG - chr1.3:25375868-25375887 None:intergenic 50.0%
ACTTTGCAAGACGGAGGAAG+AGG - chr1.3:25376027-25376046 None:intergenic 50.0%
AGTGCAAGTCAGGATGACTG+GGG + chr1.3:25376240-25376259 MS.gene063219:CDS 50.0%
ATATTTGGGCCTCGACCTTC+AGG - chr1.3:25376474-25376493 None:intergenic 50.0%
CAGTGCAAGTCAGGATGACT+GGG + chr1.3:25376239-25376258 MS.gene063219:CDS 50.0%
CATGAACGAGCAAGCCTGTA+AGG + chr1.3:25376617-25376636 MS.gene063219:CDS 50.0%
CTGCACTACTCTTCCCCTTT+GGG - chr1.3:25375867-25375886 None:intergenic 50.0%
GAATTGGGATGTGGGAGTTG+TGG + chr1.3:25376897-25376916 MS.gene063219:CDS 50.0%
GTCCTAACAGCAAAGCCTGA+AGG + chr1.3:25376456-25376475 MS.gene063219:CDS 50.0%
GTGGCTACGCAAGAGTCATA+TGG + chr1.3:25376072-25376091 MS.gene063219:intron 50.0%
TCAGTGCAAGTCAGGATGAC+TGG + chr1.3:25376238-25376257 MS.gene063219:CDS 50.0%
TCCACTAAGGACGAAAGGCT+CGG - chr1.3:25375705-25375724 None:intergenic 50.0%
! AGCTTTGTCATCCTGGAAGG+GGG + chr1.3:25376289-25376308 MS.gene063219:CDS 50.0%
! GAAGGAGAGCATGGTAGTTC+TGG + chr1.3:25376764-25376783 MS.gene063219:CDS 50.0%
! GAGCTTTGTCATCCTGGAAG+GGG + chr1.3:25376288-25376307 MS.gene063219:CDS 50.0%
! GAGTCGGTGTTTGCTGCATT+AGG + chr1.3:25376555-25376574 MS.gene063219:CDS 50.0%
!! CAGGCTTTGCTGTTAGGACT+AGG - chr1.3:25376455-25376474 None:intergenic 50.0%
!! CTGAACTCGTGGCTAGTTTC+TGG - chr1.3:25376222-25376241 None:intergenic 50.0%
!! GACCTTCAGGCTTTGCTGTT+AGG - chr1.3:25376461-25376480 None:intergenic 50.0%
!! GGTAGTTCTGGAGCTCGAAT+TGG + chr1.3:25376776-25376795 MS.gene063219:CDS 50.0%
ACAGCAAAGCCTGAAGGTCG+AGG + chr1.3:25376462-25376481 MS.gene063219:CDS 55.0%
CCACGAGTTCAGTGCAAGTC+AGG + chr1.3:25376230-25376249 MS.gene063219:CDS 55.0%
TCCGAGCCTTTCGTCCTTAG+TGG + chr1.3:25375701-25375720 MS.gene063219:CDS 55.0%
! CCTGACTTGCACTGAACTCG+TGG - chr1.3:25376233-25376252 None:intergenic 55.0%
! GGCTTGCTCGTTCATGTCTC+TGG - chr1.3:25376613-25376632 None:intergenic 55.0%
!! GCAAACACCGACTCTGCGTC+AGG - chr1.3:25376549-25376568 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 25375611 25376926 25375611 ID=MS.gene063219
chr1.3 mRNA 25375611 25376926 25375611 ID=MS.gene063219.t1;Parent=MS.gene063219
chr1.3 exon 25375611 25375732 25375611 ID=MS.gene063219.t1.exon1;Parent=MS.gene063219.t1
chr1.3 CDS 25375611 25375732 25375611 ID=cds.MS.gene063219.t1;Parent=MS.gene063219.t1
chr1.3 exon 25375849 25376012 25375849 ID=MS.gene063219.t1.exon2;Parent=MS.gene063219.t1
chr1.3 CDS 25375849 25376012 25375849 ID=cds.MS.gene063219.t1;Parent=MS.gene063219.t1
chr1.3 exon 25376223 25376926 25376223 ID=MS.gene063219.t1.exon3;Parent=MS.gene063219.t1
chr1.3 CDS 25376223 25376926 25376223 ID=cds.MS.gene063219.t1;Parent=MS.gene063219.t1
Gene Sequence

>MS.gene063219

ATGGAGGGTCAAGAAACTAGTGTTGAGATATTTGAGAAGTTCACATGGAAGATTGAAAATTTTTCCCACTTGAATGTTGATAAGTTATACTCCGAGCCTTTCGTCCTTAGTGGATATCCATGGTAAGTTATTTGAATGAATCACAATTTCCACTGGTGGATATATAGATTAATAGATTATACACTCTTTGACATGATTTATTACATTTGTGTTTTGATCTTATTTGGTTTTGTTGTAGGAGGATTCTTCTCTTCCCAAAGGGGAAGAGTAGTGCAGTCGACCAGTTACATATATATTTGGAGGCTATGAAGACTGCTAATATGTCTGAGGGATGGAAGAGAAATGCGAAATTAAAGTTTGCTGTATTCAATCAAGTCGAGGACAACAGAACAATCACAAAAGGTTTTCTTTCTCCTCTTCCTCCGTCTTGCAAAGTGATTAAGTGTATGTTTGATTTCATTGTGGCTACGCAAGAGTCATATGGTGATCCACTATAATTTTGCAGAAGTTACAAAGTGAAAAAGAGACTGATGTGATATAAATGATAGTAATTTGACTCAAGTAAGTAAACAAAAAGAAACTAAATTTGACTCTTATGCTTTATCTTGCCAGAAACTAGCCACGAGTTCAGTGCAAGTCAGGATGACTGGGGATATTCTTCTTTCATGACATTAGTTGAGCTTTGTCATCCTGGAAGGGGGTTTATTGTGAATGATACTTGTATTGTTGGTGTGGAAATTTTTGTTTGTAAGTCAACTCATGAGAAACGAATAAATCAAATCGTTAACATCCCTTCATCCCTTACATTTGGTAGTCAAACTGGTCAAATGGAAGTGGAGCTCCTAGTCCTAACAGCAAAGCCTGAAGGTCGAGGCCCAAATATTGAAACTCTTTTACAAGTTTCTTCACTAGTTTCTACTGAGGCTACTAAAGATCCTGACGCAGAGTCGGTGTTTGCTGCATTAGGTAGAGTTATGTATTTCCTTAAGACTAGAAAAGCCAGAGACATGAACGAGCAAGCCTGTAAGGATCTTCAAGTTTTATGGGAGGAACTTGCAAAATTTAAATATGATATTACTTGGCTTGAATTGCATGTTCATTATGCTTTAGGGATAAAAAGATATGTGGAGAAAGCTTTGGAGGCTGAAAAGTTGAAGGAGAGCATGGTAGTTCTGGAGCTCGAATTGGAAAGGCTAAAGGTCAAGTCGGTTGCAACTAAGATGAATCTTGACATAGAAAGAGACTTGTTGAAATCAAAAGGTTTCAAAGAAGTAGATTTAGATTCTGAATTGGGATGTGGGAGTTGTGGACCATAG
Protein sequence

>MS.gene063219.t1

MEGQETSVEIFEKFTWKIENFSHLNVDKLYSEPFVLSGYPWRILLFPKGKSSAVDQLHIYLEAMKTANMSEGWKRNAKLKFAVFNQVEDNRTITKETSHEFSASQDDWGYSSFMTLVELCHPGRGFIVNDTCIVGVEIFVCKSTHEKRINQIVNIPSSLTFGSQTGQMEVELLVLTAKPEGRGPNIETLLQVSSLVSTEATKDPDAESVFAALGRVMYFLKTRKARDMNEQACKDLQVLWEELAKFKYDITWLELHVHYALGIKRYVEKALEAEKLKESMVVLELELERLKVKSVATKMNLDIERDLLKSKGFKEVDLDSELGCGSCGP