AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063876


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063876.t1 MTR_8g042000 87.129 101 13 0 1 101 1 101 2.01e-58 184
MS.gene063876.t1 MTR_8g042000 87.129 101 13 0 1 101 1 101 5.75e-57 184
MS.gene063876.t1 MTR_8g042040 79.208 101 21 0 1 101 1 101 5.87e-52 170
MS.gene063876.t1 MTR_8g042020 79.208 101 21 0 1 101 1 101 1.35e-50 167
MS.gene063876.t1 MTR_8g042060 76.238 101 24 0 1 101 1 101 2.93e-49 163
MS.gene063876.t1 MTR_8g042060 76.238 101 24 0 1 101 1 101 9.62e-49 162
MS.gene063876.t1 MTR_2g072400 40.000 95 52 2 8 101 2 92 5.24e-16 72.4
MS.gene063876.t1 MTR_2g072330 36.634 101 60 1 8 108 3 99 1.55e-15 71.2
MS.gene063876.t1 MTR_2g055430 38.318 107 60 5 1 107 1 101 5.36e-14 66.6
MS.gene063876.t1 MTR_2g055430 38.318 107 60 5 1 107 1 101 7.68e-14 66.2
MS.gene063876.t1 MTR_2g072340 37.000 100 59 1 8 107 3 98 1.07e-13 65.9
MS.gene063876.t1 MTR_2g055470 36.036 111 65 5 1 111 1 105 6.85e-13 63.5
MS.gene063876.t1 MTR_2g072270 37.000 100 52 3 9 101 6 101 9.44e-13 63.2
MS.gene063876.t1 MTR_5g095230 36.170 94 51 3 10 101 5 91 4.69e-12 61.2
MS.gene063876.t1 MTR_2g072410 30.108 93 61 1 11 103 6 94 6.21e-12 57.4
MS.gene063876.t1 MTR_5g095230 36.170 94 51 3 10 101 5 91 6.29e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATAACCTTTCAGTATTAA+AGG 0.250524 8.1:+61984824 MS.gene063876:CDS
GATAGATGGTGCAACTTCTT+TGG 0.289005 8.1:-61984913 None:intergenic
TTTAATACTGAAAGGTTATA+TGG 0.289696 8.1:-61984822 None:intergenic
AGAAGCTTCTCTAACCTCTT+TGG 0.301833 8.1:-61984774 None:intergenic
GAGAAGCTTCTCAAAGCTTA+TGG 0.318867 8.1:+61984786 MS.gene063876:CDS
CATCAAGCTGTACACAAATA+TGG 0.350284 8.1:+61984948 MS.gene063876:CDS
CTCAAAGCTTATGGTCTTCA+AGG 0.386697 8.1:+61984795 MS.gene063876:CDS
ATTTACACTTATCTTCTTGT+TGG 0.391812 8.1:-61984878 None:intergenic
AAGTAGAACTGTTAGAAAAC+AGG 0.419889 8.1:-61984709 None:intergenic
GTGATTATTGTTGTTGTTGT+GGG 0.438409 8.1:-61984687 None:intergenic
TGAAGAGGTGAGAAGATAGA+TGG 0.483390 8.1:-61984927 None:intergenic
TTCTACTTGCTGTGATTCCA+TGG 0.490272 8.1:+61984724 MS.gene063876:CDS
ATTTGTCCTTTAATACTGAA+AGG 0.527320 8.1:-61984830 None:intergenic
GGTGATTATTGTTGTTGTTG+TGG 0.528693 8.1:-61984688 None:intergenic
GAAGATAAGTGTAAATCCAT+TGG 0.534390 8.1:+61984885 MS.gene063876:CDS
AACTTCTTTGGTGAGACCAA+TGG 0.558077 8.1:-61984901 None:intergenic
TTGTGTACAGCTTGATGAAG+AGG 0.584589 8.1:-61984942 None:intergenic
AAATTGTTGAAGAGATGCCA+TGG 0.587822 8.1:-61984741 None:intergenic
TTGCAACTGTAAGAAAATGC+AGG 0.592722 8.1:-61986386 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAATGTTAATATCAAATA+AGG + chr8.1:61985776-61985795 MS.gene063876:intron 15.0%
!!! TTATTTTTATTTTTTGCCTT+GGG - chr8.1:61985214-61985233 None:intergenic 15.0%
!!! TTTATTTTTATTTTTTGCCT+TGG - chr8.1:61985215-61985234 None:intergenic 15.0%
!!! TTTTCTTTTCAATTATACAA+GGG - chr8.1:61985694-61985713 None:intergenic 15.0%
!!! TTTTTCTTTTCAATTATACA+AGG - chr8.1:61985695-61985714 None:intergenic 15.0%
!! AATCAAATTAACCATTAGTA+AGG + chr8.1:61985494-61985513 MS.gene063876:intron 20.0%
!! ACATAGAAAAATTGAAAGTA+AGG + chr8.1:61986272-61986291 MS.gene063876:intron 20.0%
!! ATATAACCTTTCAGTATTAA+AGG + chr8.1:61984824-61984843 MS.gene063876:CDS 20.0%
!! ATGATTTACATTATACTGTA+GGG + chr8.1:61986356-61986375 MS.gene063876:intron 20.0%
!! TATGATTTACATTATACTGT+AGG + chr8.1:61986355-61986374 MS.gene063876:intron 20.0%
!! TCCATTATAAGATAGTATTA+AGG - chr8.1:61985413-61985432 None:intergenic 20.0%
!! TTCTCATTGTTTATTAATAC+CGG + chr8.1:61985067-61985086 MS.gene063876:intron 20.0%
!! TTTAATACTGAAAGGTTATA+TGG - chr8.1:61984825-61984844 None:intergenic 20.0%
!!! AAAGTTTTAAAAAGTGTTAG+TGG - chr8.1:61985624-61985643 None:intergenic 20.0%
!!! AATTTGTTGTTCTAACATAA+AGG + chr8.1:61986036-61986055 MS.gene063876:intron 20.0%
!!! TATTTTTATTTTTTGCCTTG+GGG - chr8.1:61985213-61985232 None:intergenic 20.0%
!!! TCAATTTTGATTGTTATTGA+TGG + chr8.1:61985008-61985027 MS.gene063876:intron 20.0%
!!! TTACTTTCAATTTTTCTATG+TGG - chr8.1:61986273-61986292 None:intergenic 20.0%
!!! TTTGTAATTTTTTCCTTAGA+AGG - chr8.1:61985142-61985161 None:intergenic 20.0%
! AACATAAAGGATATATATGC+AGG + chr8.1:61986049-61986068 MS.gene063876:intron 25.0%
! AGAAACAAATTCCTTACTAA+TGG - chr8.1:61985508-61985527 None:intergenic 25.0%
! ATTTACACTTATCTTCTTGT+TGG - chr8.1:61984881-61984900 None:intergenic 25.0%
! ATTTGTCCTTTAATACTGAA+AGG - chr8.1:61984833-61984852 None:intergenic 25.0%
! GCCTTAATACTATCTTATAA+TGG + chr8.1:61985409-61985428 MS.gene063876:intron 25.0%
! GTTTATCTATCTGTTTACTT+TGG + chr8.1:61986003-61986022 MS.gene063876:intron 25.0%
! TCTCATTGTTTATTAATACC+GGG + chr8.1:61985068-61985087 MS.gene063876:intron 25.0%
!! AAATCCATTTCCAAGTATTT+TGG + chr8.1:61986181-61986200 MS.gene063876:intron 25.0%
!! AGGAAAGAATTCCTTTTTAT+GGG + chr8.1:61986069-61986088 MS.gene063876:intron 25.0%
!!! ATTTTTATTTTTTGCCTTGG+GGG - chr8.1:61985212-61985231 None:intergenic 25.0%
!!! GGTTTTGTAGTTTTTTTCTT+AGG + chr8.1:61985336-61985355 MS.gene063876:intron 25.0%
AACACCAAAATACTTGGAAA+TGG - chr8.1:61986188-61986207 None:intergenic 30.0%
AAGTAGAACTGTTAGAAAAC+AGG - chr8.1:61984712-61984731 None:intergenic 30.0%
AATGTAAACTAAACTCCTCT+AGG - chr8.1:61985726-61985745 None:intergenic 30.0%
AGTTTACATTATCAGATGCA+AGG + chr8.1:61985736-61985755 MS.gene063876:intron 30.0%
ATCACAACTTTGTGAAAGTT+AGG + chr8.1:61985366-61985385 MS.gene063876:intron 30.0%
ATCGCAACTTCGTAAAAATT+AGG - chr8.1:61985114-61985133 None:intergenic 30.0%
CATTAAGTCCATTTCATGTT+TGG - chr8.1:61985763-61985782 None:intergenic 30.0%
GAAGATAAGTGTAAATCCAT+TGG + chr8.1:61984885-61984904 MS.gene063876:CDS 30.0%
GTTTCTTCATAATTGTGTTG+CGG + chr8.1:61985522-61985541 MS.gene063876:intron 30.0%
TATACCAACACCAAAATACT+TGG - chr8.1:61986194-61986213 None:intergenic 30.0%
TATCTCTTCAATCTCAATTG+AGG - chr8.1:61985568-61985587 None:intergenic 30.0%
TCGCAACTTCGTAAAAATTA+GGG - chr8.1:61985113-61985132 None:intergenic 30.0%
TGACATGATTCTCAACTAAA+AGG + chr8.1:61985655-61985674 MS.gene063876:intron 30.0%
! AAGAATTCCTTTTTATGGGA+CGG + chr8.1:61986073-61986092 MS.gene063876:intron 30.0%
! AAGACTTCCTTAATTTGATC+AGG - chr8.1:61986127-61986146 None:intergenic 30.0%
! AATTTTTCTATGTGGAATGC+CGG - chr8.1:61986265-61986284 None:intergenic 30.0%
! AGACTTCCTTAATTTGATCA+GGG - chr8.1:61986126-61986145 None:intergenic 30.0%
! CAGGAAAGAATTCCTTTTTA+TGG + chr8.1:61986068-61986087 MS.gene063876:intron 30.0%
! CTTTCACAAAGTTGTGATTT+TGG - chr8.1:61985364-61985383 None:intergenic 30.0%
! GTGATTATTGTTGTTGTTGT+GGG - chr8.1:61984690-61984709 None:intergenic 30.0%
!!! ATGGATTTTAACTCTGGTTT+AGG - chr8.1:61986169-61986188 None:intergenic 30.0%
!!! ATTTCCAAGTATTTTGGTGT+TGG + chr8.1:61986187-61986206 MS.gene063876:intron 30.0%
!!! ATTTTTGGCTTAAACCAAAG+AGG + chr8.1:61984760-61984779 MS.gene063876:CDS 30.0%
!!! TGGATTTTAACTCTGGTTTA+GGG - chr8.1:61986168-61986187 None:intergenic 30.0%
!!! TTGGAAATGGATTTTAACTC+TGG - chr8.1:61986175-61986194 None:intergenic 30.0%
AAATTGTTGAAGAGATGCCA+TGG - chr8.1:61984744-61984763 None:intergenic 35.0%
ACAACTTTGTGAAAGTTAGG+TGG + chr8.1:61985369-61985388 MS.gene063876:intron 35.0%
ACATCGAAAGATAGTCAATC+CGG + chr8.1:61986243-61986262 MS.gene063876:intron 35.0%
CAACTTTGTGAAAGTTAGGT+GGG + chr8.1:61985370-61985389 MS.gene063876:intron 35.0%
CAGATATACGTAGACACAAT+TGG + chr8.1:61985946-61985965 MS.gene063876:intron 35.0%
CATCAAGCTGTACACAAATA+TGG + chr8.1:61984948-61984967 MS.gene063876:CDS 35.0%
CGCAACTTCGTAAAAATTAG+GGG - chr8.1:61985112-61985131 None:intergenic 35.0%
GCAACTTCGTAAAAATTAGG+GGG - chr8.1:61985111-61985130 None:intergenic 35.0%
TGCTACAGTTGCAAAACTTA+GGG - chr8.1:61985171-61985190 None:intergenic 35.0%
TTGCAACTGTAAGAAAATGC+AGG - chr8.1:61986389-61986408 None:intergenic 35.0%
! CAACCTAAGTTTTGCAACTA+TGG - chr8.1:61985315-61985334 None:intergenic 35.0%
! GGCATCTCTTCAACAATTTT+TGG + chr8.1:61984745-61984764 MS.gene063876:CDS 35.0%
! GGTGATTATTGTTGTTGTTG+TGG - chr8.1:61984691-61984710 None:intergenic 35.0%
! TAGTTGCAAAACTTAGGTTG+CGG + chr8.1:61985315-61985334 MS.gene063876:intron 35.0%
! TGATGGTACTTTACCGATTA+TGG + chr8.1:61985025-61985044 MS.gene063876:intron 35.0%
! TTTTGCAACTATGGCAGTTT+TGG - chr8.1:61985306-61985325 None:intergenic 35.0%
! TTTTGCAACTGTAGCAGTTA+TGG + chr8.1:61985175-61985194 MS.gene063876:intron 35.0%
!! CTATCTGTTTACTTTGGAGT+CGG + chr8.1:61986009-61986028 MS.gene063876:intron 35.0%
!! TATCTGTTTACTTTGGAGTC+GGG + chr8.1:61986010-61986029 MS.gene063876:intron 35.0%
AACATGTAGCACCTCACTTA+GGG + chr8.1:61985240-61985259 MS.gene063876:intron 40.0%
AACTTCTTTGGTGAGACCAA+TGG - chr8.1:61984904-61984923 None:intergenic 40.0%
AATAGAGCAAGTAGAGCTGA+AGG + chr8.1:61985832-61985851 MS.gene063876:intron 40.0%
AATTCCCCAACGACCATAAT+CGG - chr8.1:61985041-61985060 None:intergenic 40.0%
ACGTATATCTGAGAGCAAAG+TGG - chr8.1:61985937-61985956 None:intergenic 40.0%
AGAAAAAAGCAGAGTCCTAG+AGG + chr8.1:61985708-61985727 MS.gene063876:intron 40.0%
AGAAGCTTCTCTAACCTCTT+TGG - chr8.1:61984777-61984796 None:intergenic 40.0%
AGCATAGACCGAGTCAAAAT+TGG + chr8.1:61985272-61985291 MS.gene063876:intron 40.0%
CACAGACCCTGATCAAATTA+AGG + chr8.1:61986117-61986136 MS.gene063876:intron 40.0%
CTACAGTTGCAAAACTTAGG+GGG - chr8.1:61985169-61985188 None:intergenic 40.0%
CTCAAAGCTTATGGTCTTCA+AGG + chr8.1:61984795-61984814 MS.gene063876:CDS 40.0%
CTGCCATAGTTGCAAAACTT+AGG + chr8.1:61985309-61985328 MS.gene063876:intron 40.0%
CTGCTACAGTTGCAAAACTT+AGG - chr8.1:61985172-61985191 None:intergenic 40.0%
GAGAAGCTTCTCAAAGCTTA+TGG + chr8.1:61984786-61984805 MS.gene063876:CDS 40.0%
GATAGATGGTGCAACTTCTT+TGG - chr8.1:61984916-61984935 None:intergenic 40.0%
GCGAAAACTACTATTAAGCC+CGG - chr8.1:61985089-61985108 None:intergenic 40.0%
GCTACAGTTGCAAAACTTAG+GGG - chr8.1:61985170-61985189 None:intergenic 40.0%
GGGTCTGTGATGATAACATT+TGG - chr8.1:61986106-61986125 None:intergenic 40.0%
TAACATGTAGCACCTCACTT+AGG + chr8.1:61985239-61985258 MS.gene063876:intron 40.0%
TGAAGAGGTGAGAAGATAGA+TGG - chr8.1:61984930-61984949 None:intergenic 40.0%
TGCAAGGACCAAACATGAAA+TGG + chr8.1:61985752-61985771 MS.gene063876:intron 40.0%
TTGTGTACAGCTTGATGAAG+AGG - chr8.1:61984945-61984964 None:intergenic 40.0%
! ACTTTACCGATTATGGTCGT+TGG + chr8.1:61985032-61985051 MS.gene063876:intron 40.0%
! CTTTACCGATTATGGTCGTT+GGG + chr8.1:61985033-61985052 MS.gene063876:intron 40.0%
! TTTACCGATTATGGTCGTTG+GGG + chr8.1:61985034-61985053 MS.gene063876:intron 40.0%
!! ACCGAGTCAAAATTGGCTTT+GGG + chr8.1:61985279-61985298 MS.gene063876:intron 40.0%
!! GTTGCGATTTTGACCTTCTA+AGG + chr8.1:61985126-61985145 MS.gene063876:intron 40.0%
!! TTCTACTTGCTGTGATTCCA+TGG + chr8.1:61984724-61984743 MS.gene063876:CDS 40.0%
!!! AGTGGCTGTGATTTTAGTTG+CGG - chr8.1:61985606-61985625 None:intergenic 40.0%
!!! TCCCAAAGCCAATTTTGACT+CGG - chr8.1:61985283-61985302 None:intergenic 40.0%
ACACCTAGAGGGTGAGAAAT+GGG + chr8.1:61986216-61986235 MS.gene063876:intron 45.0%
CGGTGAATTGCTAGTAGATG+CGG + chr8.1:61985542-61985561 MS.gene063876:intron 45.0%
GTTGGTATAGCACACCTAGA+GGG + chr8.1:61986205-61986224 MS.gene063876:intron 45.0%
TAGCAGTTATGGCGCAGTTA+TGG + chr8.1:61985186-61985205 MS.gene063876:intron 45.0%
TGTTGCACCGTCCCATAAAA+AGG - chr8.1:61986083-61986102 None:intergenic 45.0%
TGTTGGTATAGCACACCTAG+AGG + chr8.1:61986204-61986223 MS.gene063876:intron 45.0%
! GACCGAGTCAAAATTGGCTT+TGG + chr8.1:61985278-61985297 MS.gene063876:intron 45.0%
CACACCTAGAGGGTGAGAAA+TGG + chr8.1:61986215-61986234 MS.gene063876:intron 50.0%
CAGTTGCAAAACTTAGGGGG+CGG - chr8.1:61985166-61985185 None:intergenic 50.0%
TAGCCCATTTCTCACCCTCT+AGG - chr8.1:61986222-61986241 None:intergenic 50.0%
! TGAAGGCACAACATGCGTAG+CGG + chr8.1:61985849-61985868 MS.gene063876:intron 50.0%
CTGACGTGACACCCTAAGTG+AGG - chr8.1:61985254-61985273 None:intergenic 55.0%
TGGCGCAGTTATGGCCCCCA+AGG + chr8.1:61985195-61985214 MS.gene063876:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 61984669 61986411 61984669 ID=MS.gene063876
chr8.1 mRNA 61984669 61986411 61984669 ID=MS.gene063876.t1;Parent=MS.gene063876
chr8.1 exon 61984669 61984969 61984669 ID=MS.gene063876.t1.exon1;Parent=MS.gene063876.t1
chr8.1 CDS 61984669 61984969 61984669 ID=cds.MS.gene063876.t1;Parent=MS.gene063876.t1
chr8.1 exon 61986377 61986411 61986377 ID=MS.gene063876.t1.exon2;Parent=MS.gene063876.t1
chr8.1 CDS 61986377 61986411 61986377 ID=cds.MS.gene063876.t1;Parent=MS.gene063876.t1
Gene Sequence

>MS.gene063876

ATGGAGTTGTTTGTGTTTCCCACAACAACAACAATAATCACCTGTTTTCTAACAGTTCTACTTGCTGTGATTCCATGGCATCTCTTCAACAATTTTTGGCTTAAACCAAAGAGGTTAGAGAAGCTTCTCAAAGCTTATGGTCTTCAAGGTGAACCATATAACCTTTCAGTATTAAAGGACAAATCAAAACAAAATTATATGTTGAAGCTCCAACAAGAAGATAAGTGTAAATCCATTGGTCTCACCAAAGAAGTTGCACCATCTATCTTCTCACCTCTTCATCAAGCTGTACACAAATATGGTACATATACTAAATATTGTATGTTGAGTTTTGAAAATTCAATTTTGATTGTTATTGATGGTACTTTACCGATTATGGTCGTTGGGGAATTACATATTTCTCATTGTTTATTAATACCGGGCTTAATAGTAGTTTTCGCCCCCTAATTTTTACGAAGTTGCGATTTTGACCTTCTAAGGAAAAAATTACAAAACCGCCCCCTAAGTTTTGCAACTGTAGCAGTTATGGCGCAGTTATGGCCCCCAAGGCAAAAAATAAAAATAAAAAAATAACATGTAGCACCTCACTTAGGGTGTCACGTCAGCATAGACCGAGTCAAAATTGGCTTTGGGACCAAAACTGCCATAGTTGCAAAACTTAGGTTGCGGTTTTGTAGTTTTTTTCTTAGGAGCCAAAATCACAACTTTGTGAAAGTTAGGTGGGCTAAAATTGCTATTAAGCCTTAATACTATCTTATAATGGATAATAACTTAAATTTCTCATTGTTTCTTCATAATTTTGTAATGAATTTGTTTCTTCTAAAAAATCAAATTAACCATTAGTAAGGAATTTGTTTCTTCATAATTGTGTTGCGGTGAATTGCTAGTAGATGCGGCCTCAATTGAGATTGAAGAGATATAAAAAATCTTGATGCCGCAACTAAAATCACAGCCACTAACACTTTTTAAAACTTTGTTAGAATATCTGACATGATTCTCAACTAAAAGGAAGTCTTATGATGCCCTTGTATAATTGAAAAGAAAAAAGCAGAGTCCTAGAGGAGTTTAGTTTACATTATCAGATGCAAGGACCAAACATGAAATGGACTTAATGTTAATATCAAATAAGGTATTCGCTGATAAGTGATAAGATGCTCTAGCGTAATAGAGCAAGTAGAGCTGAAGGCACAACATGCGTAGCGGAGTATTTAAACACTTCTTGTACTATGTAATCTTAAGCATTGCATAATAGAAAATCACAACTGCCACTTTGCTCTCAGATATACGTAGACACAATTGGAAGAACATTGTAATCAAGCATTGCATTCTTGTGCGTTTATCTATCTGTTTACTTTGGAGTCGGGTTTAATTTGTTGTTCTAACATAAAGGATATATATGCAGGAAAGAATTCCTTTTTATGGGACGGTGCAACACCAAATGTTATCATCACAGACCCTGATCAAATTAAGGAAGTCTTTAACAAGATTAACGACTTCCCTAAACCAGAGTTAAAATCCATTTCCAAGTATTTTGGTGTTGGTATAGCACACCTAGAGGGTGAGAAATGGGCTAAACATCGAAAGATAGTCAATCCGGCATTCCACATAGAAAAATTGAAAGTAAGGTTATTGCTTTAGCTAATTATTGCAATTTTGTAATTGAATTTCTAATTTTTATTCTTATAATATGATTTACATTATACTGTAGGGTATGCTGCCTGCATTTTCTTACAGTTGCAATGA

Protein sequence

>MS.gene063876.t1

MELFVFPTTTTIITCFLTVLLAVIPWHLFNNFWLKPKRLEKLLKAYGLQGEPYNLSVLKDKSKQNYMLKLQQEDKCKSIGLTKEVAPSIFSPLHQAVHKYGYAACIFLQLQ