AlfalfaGEDB | MS.gene064462

M. sativa cultivar XinJiangDaYe / MS.gene064462

KEGG: K01512
GO: GO:0003674 GO:0003824 GO:0003998 GO:0016787 GO:0016817 GO:0016818
PFAM: PF00708.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064462.t1	KEH42193.1	99	102	1	0	1	102	64	165	1.40E-51	211.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064462.t1	Q2RQZ4	57.6	92	37	1	11	102	2	91	1.3e-21	103.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064462.t1	A0A072VL74	99.0	102	1	0	1	102	64	165	1.0e-51	211.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049230	MS.gene064462	0.810397	1.18E-50	-1.69E-46
MS.gene049378	MS.gene064462	0.828555	8.42E-55	-1.69E-46
MS.gene049669	MS.gene064462	0.802898	4.51E-49	-1.69E-46
MS.gene050119	MS.gene064462	0.812366	4.40E-51	-1.69E-46
MS.gene050226	MS.gene064462	0.835961	1.24E-56	-1.69E-46
MS.gene050227	MS.gene064462	0.83592	1.27E-56	-1.69E-46
MS.gene050228	MS.gene064462	0.848071	7.74E-60	-1.69E-46
MS.gene050303	MS.gene064462	0.802726	4.89E-49	-1.69E-46
MS.gene050330	MS.gene064462	-0.823684	1.21E-53	-1.69E-46
MS.gene050442	MS.gene064462	0.812366	4.40E-51	-1.69E-46
MS.gene050623	MS.gene064462	-0.814123	1.81E-51	-1.69E-46
MS.gene050642	MS.gene064462	0.807354	5.27E-50	-1.69E-46
MS.gene050680	MS.gene064462	0.800535	1.38E-48	-1.69E-46
MS.gene050719	MS.gene064462	0.85739	1.69E-62	-1.69E-46
MS.gene050721	MS.gene064462	0.861092	1.31E-63	-1.69E-46
MS.gene050724	MS.gene064462	0.842378	2.68E-58	-1.69E-46
MS.gene050750	MS.gene064462	0.813838	2.09E-51	-1.69E-46
MS.gene050780	MS.gene064462	0.825725	4.01E-54	-1.69E-46
MS.gene050820	MS.gene064462	0.807913	4.01E-50	-1.69E-46
MS.gene050844	MS.gene064462	0.805697	1.18E-49	-1.69E-46
MS.gene051349	MS.gene064462	0.890084	1.36E-73	-1.69E-46
MS.gene051452	MS.gene064462	0.811512	6.75E-51	-1.69E-46
MS.gene051983	MS.gene064462	0.845005	5.32E-59	-1.69E-46
MS.gene052176	MS.gene064462	0.864642	1.05E-64	-1.69E-46
MS.gene052309	MS.gene064462	-0.80292	4.46E-49	-1.69E-46
MS.gene052376	MS.gene064462	0.80748	4.95E-50	-1.69E-46
MS.gene052426	MS.gene064462	0.815848	7.49E-52	-1.69E-46
MS.gene053006	MS.gene064462	0.808361	3.22E-50	-1.69E-46
MS.gene053216	MS.gene064462	0.808899	2.47E-50	-1.69E-46
MS.gene05415	MS.gene064462	0.805437	1.34E-49	-1.69E-46
MS.gene054160	MS.gene064462	0.806453	8.16E-50	-1.69E-46
MS.gene054930	MS.gene064462	0.81464	1.39E-51	-1.69E-46
MS.gene05510	MS.gene064462	0.807366	5.24E-50	-1.69E-46
MS.gene055701	MS.gene064462	0.821262	4.43E-53	-1.69E-46
MS.gene056506	MS.gene064462	0.852146	5.59E-61	-1.69E-46
MS.gene056979	MS.gene064462	0.838357	3.02E-57	-1.69E-46
MS.gene057060	MS.gene064462	0.809142	2.19E-50	-1.69E-46
MS.gene05795	MS.gene064462	0.827952	1.18E-54	-1.69E-46
MS.gene058426	MS.gene064462	0.814388	1.58E-51	-1.69E-46
MS.gene058749	MS.gene064462	0.800141	1.66E-48	-1.69E-46
MS.gene058979	MS.gene064462	0.801816	7.53E-49	-1.69E-46
MS.gene059249	MS.gene064462	0.805279	1.44E-49	-1.69E-46
MS.gene059569	MS.gene064462	0.806785	6.95E-50	-1.69E-46
MS.gene060322	MS.gene064462	0.829766	4.28E-55	-1.69E-46
MS.gene060572	MS.gene064462	0.810902	9.15E-51	-1.69E-46
MS.gene060683	MS.gene064462	0.815351	9.66E-52	-1.69E-46
MS.gene060685	MS.gene064462	0.810317	1.22E-50	-1.69E-46
MS.gene060998	MS.gene064462	0.809578	1.77E-50	-1.69E-46
MS.gene061040	MS.gene064462	-0.827967	1.17E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064462.t1	MTR_1g063240	99.020	102	1	0	1	102	64	165	3.73e-72	211

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064462.t1	AT5G03370	72.826	92	23	1	11	102	82	171	1.28e-42	137

Find 44 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCACCGGATGCTGCTGTTAC+TGG	0.095996	1.4:+52033006	MS.gene064462:CDS
GTAGGAGAGGGTGGTGATAA+AGG	0.264206	1.4:+52032822	MS.gene064462:intron
AGTGGAAAATGCGAGGGAAT+TGG	0.324078	1.4:+52032879	MS.gene064462:CDS
GCGAGGGAATTGGGGCTAAA+AGG	0.333979	1.4:+52032889	MS.gene064462:CDS
TAGGAGAGGGTGGTGATAAA+GGG	0.338790	1.4:+52032823	MS.gene064462:intron
TTAATTCACATTCACCGTTT+TGG	0.339096	1.4:-52032742	None:intergenic
GTGGAAAATGCGAGGGAATT+GGG	0.353618	1.4:+52032880	MS.gene064462:CDS
AGGGGAGAGTGCAGGGAGTA+TGG	0.368110	1.4:+52032842	MS.gene064462:CDS
CAGAACCATCCCTTCGGTTC+CGG	0.380690	1.4:-52032918	None:intergenic
CACCGGATGCTGCTGTTACT+GGG	0.395102	1.4:+52033007	MS.gene064462:CDS
GGTGATAAAGGGGAGAGTGC+AGG	0.396368	1.4:+52032834	MS.gene064462:CDS
CGTCGAAGCTGTGCAGGAAA+TGG	0.412584	1.4:+52032963	MS.gene064462:CDS
AGGGAGTATGGTACAGAAAC+TGG	0.434232	1.4:+52032854	MS.gene064462:CDS
GTGATAAAGGGGAGAGTGCA+GGG	0.447756	1.4:+52032835	MS.gene064462:CDS
ATCAAACAGTTGGTTTGCGT+TGG	0.461869	1.4:-52033077	None:intergenic
GAAGAATAATATCAAACAGT+TGG	0.476033	1.4:-52033087	None:intergenic
CGGTAGCGTCGAAGCTGTGC+AGG	0.487308	1.4:+52032957	MS.gene064462:CDS
GGGAATTGGGGCTAAAAGGA+TGG	0.488625	1.4:+52032893	MS.gene064462:CDS
CTGCACAGCTTCGACGCTAC+CGG	0.491316	1.4:-52032956	None:intergenic
AGGAGAGGGTGGTGATAAAG+GGG	0.505929	1.4:+52032824	MS.gene064462:intron
TGGAAAATGCGAGGGAATTG+GGG	0.522412	1.4:+52032881	MS.gene064462:CDS
GATGATGATGATCCTGGTAC+TGG	0.523433	1.4:+52033051	MS.gene064462:CDS
AGCCCAGTAACAGCAGCATC+CGG	0.529354	1.4:-52033009	None:intergenic
GAGGTGTCGTCGTGGTCCAC+CGG	0.535871	1.4:+52032990	MS.gene064462:CDS
TGCTGCTGTTACTGGGCTTG+AGG	0.546519	1.4:+52033014	MS.gene064462:CDS
TGGGGCTAAAAGGATGGGTC+CGG	0.556862	1.4:+52032899	MS.gene064462:CDS
AGATTCCTCTTCTACCAAAA+CGG	0.559157	1.4:+52032728	MS.gene064462:CDS
TGGGTCCGGAACCGAAGGGA+TGG	0.559523	1.4:+52032913	MS.gene064462:CDS
AAGGATGGGTCCGGAACCGA+AGG	0.565332	1.4:+52032908	MS.gene064462:CDS
TTGCGTTGGAAACCAGTACC+AGG	0.567923	1.4:-52033063	None:intergenic
CCTCCACAGAACCATCCCTT+CGG	0.568545	1.4:-52032924	None:intergenic
GAACCGAAGGGATGGTTCTG+TGG	0.597455	1.4:+52032921	MS.gene064462:CDS
TGTGGTGATGATGATGATCC+TGG	0.597979	1.4:+52033045	MS.gene064462:CDS
GGAATTGGGGCTAAAAGGAT+GGG	0.617204	1.4:+52032894	MS.gene064462:CDS
CCGAAGGGATGGTTCTGTGG+AGG	0.635926	1.4:+52032924	MS.gene064462:CDS
CTGTGCAGGAAATGGAGAGG+AGG	0.648986	1.4:+52032971	MS.gene064462:CDS
CTGGACAGTGGAAAATGCGA+GGG	0.652835	1.4:+52032873	MS.gene064462:CDS
ACTGGACAGTGGAAAATGCG+AGG	0.658155	1.4:+52032872	MS.gene064462:CDS
AAGCTGTGCAGGAAATGGAG+AGG	0.662085	1.4:+52032968	MS.gene064462:CDS
CCAGTAACAGCAGCATCCGG+TGG	0.670473	1.4:-52033006	None:intergenic
ATGGAGAGGAGGTGTCGTCG+TGG	0.682583	1.4:+52032982	MS.gene064462:CDS
ATGGTACAGAAACTGGACAG+TGG	0.698689	1.4:+52032861	MS.gene064462:CDS
GGATCATCATCATCACCACA+TGG	0.708512	1.4:-52033042	None:intergenic
AGGATGGGTCCGGAACCGAA+GGG	0.721893	1.4:+52032909	MS.gene064462:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GATTAATATTTGTTTTTTGT+AGG	+	chr1.4:52032804-52032823	MS.gene064462:intron	15.0%
!!!	ATATTTGTTTTTTGTAGGAG+AGG	+	chr1.4:52032809-52032828	MS.gene064462:intron	25.0%
!!!	TATTTGTTTTTTGTAGGAGA+GGG	+	chr1.4:52032810-52032829	MS.gene064462:intron	25.0%
!	TTAATTCACATTCACCGTTT+TGG	-	chr1.4:52032745-52032764	None:intergenic	30.0%
	AGATTCCTCTTCTACCAAAA+CGG	+	chr1.4:52032728-52032747	MS.gene064462:CDS	35.0%
!!	ATTCACCGTTTTGGTAGAAG+AGG	-	chr1.4:52032736-52032755	None:intergenic	40.0%
!!!	TTGTTTTTTGTAGGAGAGGG+TGG	+	chr1.4:52032813-52032832	MS.gene064462:intron	40.0%
	AGTGGAAAATGCGAGGGAAT+TGG	+	chr1.4:52032879-52032898	MS.gene064462:CDS	45.0%
	ATGGTACAGAAACTGGACAG+TGG	+	chr1.4:52032861-52032880	MS.gene064462:CDS	45.0%
	GGAATTGGGGCTAAAAGGAT+GGG	+	chr1.4:52032894-52032913	MS.gene064462:CDS	45.0%
	GGATCATCATCATCACCACA+TGG	-	chr1.4:52033045-52033064	None:intergenic	45.0%
	GTGGAAAATGCGAGGGAATT+GGG	+	chr1.4:52032880-52032899	MS.gene064462:CDS	45.0%
	TAGGAGAGGGTGGTGATAAA+GGG	+	chr1.4:52032823-52032842	MS.gene064462:intron	45.0%
	TGGAAAATGCGAGGGAATTG+GGG	+	chr1.4:52032881-52032900	MS.gene064462:CDS	45.0%
!	AGGGAGTATGGTACAGAAAC+TGG	+	chr1.4:52032854-52032873	MS.gene064462:CDS	45.0%
!	GATGATGATGATCCTGGTAC+TGG	+	chr1.4:52033051-52033070	MS.gene064462:CDS	45.0%
!	TGTGGTGATGATGATGATCC+TGG	+	chr1.4:52033045-52033064	MS.gene064462:CDS	45.0%
!!	ATTAATTAAGTAATTTATTA+TGG	+	chr1.4:52032779-52032798	MS.gene064462:intron	5.0%
	AAGCTGTGCAGGAAATGGAG+AGG	+	chr1.4:52032968-52032987	MS.gene064462:CDS	50.0%
	ACTGGACAGTGGAAAATGCG+AGG	+	chr1.4:52032872-52032891	MS.gene064462:CDS	50.0%
	AGGAGAGGGTGGTGATAAAG+GGG	+	chr1.4:52032824-52032843	MS.gene064462:intron	50.0%
	CTGGACAGTGGAAAATGCGA+GGG	+	chr1.4:52032873-52032892	MS.gene064462:CDS	50.0%
	GGGAATTGGGGCTAAAAGGA+TGG	+	chr1.4:52032893-52032912	MS.gene064462:CDS	50.0%
	GTAGGAGAGGGTGGTGATAA+AGG	+	chr1.4:52032822-52032841	MS.gene064462:intron	50.0%
	TTGCGTTGGAAACCAGTACC+AGG	-	chr1.4:52033066-52033085	None:intergenic	50.0%
!	GGGCTTGAGGTTTTTCCATG+TGG	+	chr1.4:52033027-52033046	MS.gene064462:CDS	50.0%
!	GTGATAAAGGGGAGAGTGCA+GGG	+	chr1.4:52032835-52032854	MS.gene064462:CDS	50.0%
!	TCTGTGGAGGCTCTGTTTTC+CGG	+	chr1.4:52032937-52032956	MS.gene064462:CDS	50.0%
	AGCCCAGTAACAGCAGCATC+CGG	-	chr1.4:52033012-52033031	None:intergenic	55.0%
	CACCGGATGCTGCTGTTACT+GGG	+	chr1.4:52033007-52033026	MS.gene064462:CDS	55.0%
	CAGAACCATCCCTTCGGTTC+CGG	-	chr1.4:52032921-52032940	None:intergenic	55.0%
	CCTCCACAGAACCATCCCTT+CGG	-	chr1.4:52032927-52032946	None:intergenic	55.0%
	CGTCGAAGCTGTGCAGGAAA+TGG	+	chr1.4:52032963-52032982	MS.gene064462:CDS	55.0%
	CTGTGCAGGAAATGGAGAGG+AGG	+	chr1.4:52032971-52032990	MS.gene064462:CDS	55.0%
	GCGAGGGAATTGGGGCTAAA+AGG	+	chr1.4:52032889-52032908	MS.gene064462:CDS	55.0%
	TGCTGCTGTTACTGGGCTTG+AGG	+	chr1.4:52033014-52033033	MS.gene064462:CDS	55.0%
	TGGGGCTAAAAGGATGGGTC+CGG	+	chr1.4:52032899-52032918	MS.gene064462:CDS	55.0%
!	GAACCGAAGGGATGGTTCTG+TGG	+	chr1.4:52032921-52032940	MS.gene064462:CDS	55.0%
!	GGTGATAAAGGGGAGAGTGC+AGG	+	chr1.4:52032834-52032853	MS.gene064462:CDS	55.0%
	AAGGATGGGTCCGGAACCGA+AGG	+	chr1.4:52032908-52032927	MS.gene064462:CDS	60.0%
	AGGATGGGTCCGGAACCGAA+GGG	+	chr1.4:52032909-52032928	MS.gene064462:CDS	60.0%
	CCACCGGATGCTGCTGTTAC+TGG	+	chr1.4:52033006-52033025	MS.gene064462:CDS	60.0%
	CCAGTAACAGCAGCATCCGG+TGG	-	chr1.4:52033009-52033028	None:intergenic	60.0%
	CTGCACAGCTTCGACGCTAC+CGG	-	chr1.4:52032959-52032978	None:intergenic	60.0%
!	AGGGGAGAGTGCAGGGAGTA+TGG	+	chr1.4:52032842-52032861	MS.gene064462:CDS	60.0%
!	CCGAAGGGATGGTTCTGTGG+AGG	+	chr1.4:52032924-52032943	MS.gene064462:CDS	60.0%
!!	ATGGAGAGGAGGTGTCGTCG+TGG	+	chr1.4:52032982-52033001	MS.gene064462:CDS	60.0%
	TGGGTCCGGAACCGAAGGGA+TGG	+	chr1.4:52032913-52032932	MS.gene064462:CDS	65.0%
!	CGGTAGCGTCGAAGCTGTGC+AGG	+	chr1.4:52032957-52032976	MS.gene064462:CDS	65.0%
!!	GAGGTGTCGTCGTGGTCCAC+CGG	+	chr1.4:52032990-52033009	MS.gene064462:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	52032714	52033098	52032714	ID=MS.gene064462
chr1.4	mRNA	52032714	52033098	52032714	ID=MS.gene064462.t1;Parent=MS.gene064462
chr1.4	exon	52032714	52032749	52032714	ID=MS.gene064462.t1.exon1;Parent=MS.gene064462.t1
chr1.4	CDS	52032714	52032749	52032714	ID=cds.MS.gene064462.t1;Parent=MS.gene064462.t1
chr1.4	exon	52032826	52033098	52032826	ID=MS.gene064462.t1.exon2;Parent=MS.gene064462.t1
chr1.4	CDS	52032826	52033098	52032826	ID=cds.MS.gene064462.t1;Parent=MS.gene064462.t1

Gene Sequence

>MS.gene064462

ATGGCGAGTTCTCAAGATTCCTCTTCTACCAAAACGGTGAATGTGAATTAATTGATTCAAATTTAATTAATTAAGTAATTTATTATGGTTGATTAATATTTGTTTTTTGTAGGAGAGGGTGGTGATAAAGGGGAGAGTGCAGGGAGTATGGTACAGAAACTGGACAGTGGAAAATGCGAGGGAATTGGGGCTAAAAGGATGGGTCCGGAACCGAAGGGATGGTTCTGTGGAGGCTCTGTTTTCCGGTAGCGTCGAAGCTGTGCAGGAAATGGAGAGGAGGTGTCGTCGTGGTCCACCGGATGCTGCTGTTACTGGGCTTGAGGTTTTTCCATGTGGTGATGATGATGATCCTGGTACTGGTTTCCAACGCAAACCAACTGTTTGA

Protein sequence

>MS.gene064462.t1

MASSQDSSSTKTERVVIKGRVQGVWYRNWTVENARELGLKGWVRNRRDGSVEALFSGSVEAVQEMERRCRRGPPDAAVTGLEVFPCGDDDDPGTGFQRKPTV