Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066337.t1 | XP_003615100.1 | 94 | 67 | 4 | 0 | 1 | 67 | 10 | 76 | 6.20E-27 | 129.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066337.t1 | G7KB77 | 94.0 | 67 | 4 | 0 | 1 | 67 | 10 | 76 | 4.5e-27 | 129.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene066337 | 0.814554 | 1.45E-51 | -1.69E-46 |
| MS.gene049151 | MS.gene066337 | 0.846606 | 1.95E-59 | -1.69E-46 |
| MS.gene049153 | MS.gene066337 | 0.80721 | 5.65E-50 | -1.69E-46 |
| MS.gene049260 | MS.gene066337 | 0.819953 | 8.86E-53 | -1.69E-46 |
| MS.gene049758 | MS.gene066337 | 0.826331 | 2.88E-54 | -1.69E-46 |
| MS.gene049915 | MS.gene066337 | 0.833075 | 6.57E-56 | -1.69E-46 |
| MS.gene050128 | MS.gene066337 | 0.813499 | 2.48E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene066337 | 0.827774 | 1.30E-54 | -1.69E-46 |
| MS.gene050227 | MS.gene066337 | 0.811459 | 6.93E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene066337 | 0.812676 | 3.76E-51 | -1.69E-46 |
| MS.gene050438 | MS.gene066337 | 0.805361 | 1.39E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene066337 | -0.825787 | 3.87E-54 | -1.69E-46 |
| MS.gene050762 | MS.gene066337 | 0.801508 | 8.71E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene066337 | 0.804421 | 2.18E-49 | -1.69E-46 |
| MS.gene050815 | MS.gene066337 | 0.808728 | 2.69E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene066337 | 0.802429 | 5.63E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene066337 | 0.832928 | 7.14E-56 | -1.69E-46 |
| MS.gene052178 | MS.gene066337 | 0.818429 | 1.97E-52 | -1.69E-46 |
| MS.gene052255 | MS.gene066337 | 0.801765 | 7.72E-49 | -1.69E-46 |
| MS.gene052337 | MS.gene066337 | 0.822803 | 1.95E-53 | -1.69E-46 |
| MS.gene053074 | MS.gene066337 | 0.841801 | 3.81E-58 | -1.69E-46 |
| MS.gene053204 | MS.gene066337 | 0.807269 | 5.49E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene066337 | 0.828968 | 6.69E-55 | -1.69E-46 |
| MS.gene054184 | MS.gene066337 | 0.873626 | 1.27E-67 | -1.69E-46 |
| MS.gene054185 | MS.gene066337 | 0.875176 | 3.79E-68 | -1.69E-46 |
| MS.gene054379 | MS.gene066337 | 0.801218 | 9.99E-49 | -1.69E-46 |
| MS.gene054596 | MS.gene066337 | 0.809931 | 1.48E-50 | -1.69E-46 |
| MS.gene055133 | MS.gene066337 | 0.862355 | 5.38E-64 | -1.69E-46 |
| MS.gene055422 | MS.gene066337 | 0.806125 | 9.58E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene066337 | 0.805076 | 1.59E-49 | -1.69E-46 |
| MS.gene056875 | MS.gene066337 | 0.81223 | 4.71E-51 | -1.69E-46 |
| MS.gene057260 | MS.gene066337 | 0.818016 | 2.44E-52 | -1.69E-46 |
| MS.gene057750 | MS.gene066337 | 0.816163 | 6.37E-52 | -1.69E-46 |
| MS.gene057754 | MS.gene066337 | 0.895057 | 1.37E-75 | -1.69E-46 |
| MS.gene057794 | MS.gene066337 | 0.829077 | 6.30E-55 | -1.69E-46 |
| MS.gene057796 | MS.gene066337 | 0.809128 | 2.21E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene066337 | 0.802804 | 4.71E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene066337 | 0.811502 | 6.78E-51 | -1.69E-46 |
| MS.gene058749 | MS.gene066337 | 0.806217 | 9.16E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene066337 | 0.803109 | 4.08E-49 | -1.69E-46 |
| MS.gene059124 | MS.gene066337 | 0.853163 | 2.86E-61 | -1.69E-46 |
| MS.gene059294 | MS.gene066337 | 0.813222 | 2.86E-51 | -1.69E-46 |
| MS.gene059421 | MS.gene066337 | 0.822118 | 2.81E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene066337 | 0.816183 | 6.31E-52 | -1.69E-46 |
| MS.gene060476 | MS.gene066337 | 0.840286 | 9.53E-58 | -1.69E-46 |
| MS.gene060803 | MS.gene066337 | 0.811378 | 7.22E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene066337 | 0.815852 | 7.48E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066337.t1 | MTR_5g063720 | 94.030 | 67 | 4 | 0 | 1 | 67 | 10 | 76 | 5.22e-40 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTGATCAAGAATTCTTTA+AGG | 0.147055 | 5.4:+49584294 | MS.gene066337:CDS |
| CACCTTTGCCTTCAGATAAA+TGG | 0.203598 | 5.4:+49584377 | MS.gene066337:CDS |
| CTTCACAAAAGAAGAGAAAA+TGG | 0.266395 | 5.4:+49584257 | MS.gene066337:CDS |
| GCGAAGTTCTATATCAAATA+TGG | 0.295622 | 5.4:-49584347 | None:intergenic |
| AAGGATCTTACACATGATCT+AGG | 0.356603 | 5.4:+49584313 | MS.gene066337:CDS |
| GCAACAATACCTCACAGTTC+AGG | 0.455526 | 5.4:+49584399 | MS.gene066337:CDS |
| AAGAATTCTTGATCAGAAAG+TGG | 0.498028 | 5.4:-49584289 | None:intergenic |
| TTCACAAAAGAAGAGAAAAT+GGG | 0.522716 | 5.4:+49584258 | MS.gene066337:CDS |
| TATTGTTGCCATTTATCTGA+AGG | 0.533510 | 5.4:-49584385 | None:intergenic |
| AGGATCTTACACATGATCTA+GGG | 0.553583 | 5.4:+49584314 | MS.gene066337:CDS |
| TGTGAAGCATCAAAATCTGT+AGG | 0.557840 | 5.4:-49584241 | None:intergenic |
| TGCCATTTATCTGAAGGCAA+AGG | 0.597180 | 5.4:-49584379 | None:intergenic |
| TATTATATACCTGAACTGTG+AGG | 0.723748 | 5.4:-49584408 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCACAAAAGAAGAGAAAAT+GGG | + | chr5.4:49584258-49584277 | MS.gene066337:CDS | 25.0% |
| ! | TTCTGATCAAGAATTCTTTA+AGG | + | chr5.4:49584294-49584313 | MS.gene066337:CDS | 25.0% |
| AAGAATTCTTGATCAGAAAG+TGG | - | chr5.4:49584292-49584311 | None:intergenic | 30.0% | |
| CTTCACAAAAGAAGAGAAAA+TGG | + | chr5.4:49584257-49584276 | MS.gene066337:CDS | 30.0% | |
| GCGAAGTTCTATATCAAATA+TGG | - | chr5.4:49584350-49584369 | None:intergenic | 30.0% | |
| ! | TATTGTTGCCATTTATCTGA+AGG | - | chr5.4:49584388-49584407 | None:intergenic | 30.0% |
| !!! | TCTGTAGGAGATTCTTTTTT+TGG | - | chr5.4:49584229-49584248 | None:intergenic | 30.0% |
| AAGGATCTTACACATGATCT+AGG | + | chr5.4:49584313-49584332 | MS.gene066337:CDS | 35.0% | |
| AGGATCTTACACATGATCTA+GGG | + | chr5.4:49584314-49584333 | MS.gene066337:CDS | 35.0% | |
| ! | TGTGAAGCATCAAAATCTGT+AGG | - | chr5.4:49584244-49584263 | None:intergenic | 35.0% |
| CACCTTTGCCTTCAGATAAA+TGG | + | chr5.4:49584377-49584396 | MS.gene066337:CDS | 40.0% | |
| ! | TGCCATTTATCTGAAGGCAA+AGG | - | chr5.4:49584382-49584401 | None:intergenic | 40.0% |
| !!! | CTGAAGGCAAAGGTGTTTTG+AGG | - | chr5.4:49584372-49584391 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 49584220 | 49584420 | 49584220 | ID=MS.gene066337 |
| chr5.4 | mRNA | 49584220 | 49584420 | 49584220 | ID=MS.gene066337.t1;Parent=MS.gene066337 |
| chr5.4 | exon | 49584220 | 49584420 | 49584220 | ID=MS.gene066337.t1.exon1;Parent=MS.gene066337.t1 |
| chr5.4 | CDS | 49584220 | 49584420 | 49584220 | ID=cds.MS.gene066337.t1;Parent=MS.gene066337.t1 |
| Gene Sequence |
| Protein sequence |
