AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066865


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066865.t1 MTR_0170s0030 89.555 517 52 2 4 518 31 547 0.0 879
MS.gene066865.t1 MTR_5g053270 36.254 331 189 4 56 367 126 453 2.79e-41 159
MS.gene066865.t1 MTR_1g093850 31.214 346 199 6 56 367 133 473 1.90e-32 133
MS.gene066865.t1 MTR_2g100630 23.098 368 222 13 39 367 113 458 1.53e-14 76.6
MS.gene066865.t1 MTR_2g100630 23.098 368 222 13 39 367 72 417 2.02e-14 76.3
MS.gene066865.t1 MTR_8g014900 22.630 327 233 5 61 367 144 470 3.47e-14 75.9
MS.gene066865.t1 MTR_1g016290 40.426 94 51 1 194 282 207 300 2.49e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066865.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066865.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066865.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066865.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066865.t1 AT3G25690 30.617 454 264 9 34 448 117 558 2.29e-41 160
MS.gene066865.t1 AT3G25690 29.973 367 214 8 121 448 53 415 1.57e-30 127
MS.gene066865.t1 AT1G52080 26.837 313 214 6 50 355 133 437 2.74e-15 79.3
MS.gene066865.t1 AT1G52080 26.837 313 214 6 50 355 133 437 2.74e-15 79.3

Find 0 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATTATAAATGAGA+TGG - 16764:27225-27244 MS.gene066865:CDS 10.0%
!!! ATAATTTTAATAATACAAGA+TGG + 16764:27579-27598 MS.gene066865:intergenic 10.0%
!!! CTAAATTTTGTTAAAATAAT+AGG - 16764:28037-28056 MS.gene066865:intron 10.0%
!! AACAAAAAATAACACATAAA+AGG + 16764:26816-26835 MS.gene066865:intergenic 15.0%
!! ATTCTTATGTTCATTAATAT+GGG - 16764:27532-27551 MS.gene066865:CDS 15.0%
!! TACCTTAAATTTATTGTTTA+AGG - 16764:28096-28115 MS.gene066865:intron 15.0%
!! TATTAAAGAAAAAATAGAAG+AGG - 16764:27171-27190 MS.gene066865:CDS 15.0%
!! TATTCTTATGTTCATTAATA+TGG - 16764:27531-27550 MS.gene066865:CDS 15.0%
!!! AAGTAATGTTGATTAATTAT+AGG + 16764:27998-28017 MS.gene066865:intergenic 15.0%
!!! TAAAGACAAATCAATTTTTT+GGG + 16764:26871-26890 MS.gene066865:intergenic 15.0%
!!! TAATAAAAAGCATAATAGAA+AGG - 16764:28339-28358 MS.gene066865:CDS 15.0%
!!! TTAAAGACAAATCAATTTTT+TGG + 16764:26872-26891 MS.gene066865:intergenic 15.0%
!! AATCAACATTACTTAAGTTA+TGG - 16764:28004-28023 MS.gene066865:intron 20.0%
!! AATCCTACAAAAAAAATCAT+TGG - 16764:27947-27966 MS.gene066865:intron 20.0%
!! AGCCTTAAACAATAAATTTA+AGG + 16764:28101-28120 MS.gene066865:intergenic 20.0%
!! ATCCTACAAAAAAAATCATT+GGG - 16764:27948-27967 MS.gene066865:intron 20.0%
!! TGCTTAATAAATTGAAAAGA+TGG - 16764:28554-28573 MS.gene066865:CDS 20.0%
!! TTGTTAAAATAATAGGTAGT+AGG - 16764:28044-28063 MS.gene066865:intron 20.0%
!! TTTCAATTTATTAAGCAAAC+TGG + 16764:28551-28570 MS.gene066865:intergenic 20.0%
!!! AAACAATAAATTTAAGGTAG+AGG + 16764:28095-28114 MS.gene066865:intergenic 20.0%
! AAATTGGTTATTCAGATGAT+AGG - 16764:29001-29020 MS.gene066865:intron 25.0%
! AATACAAAATTACAAGCAGA+GGG - 16764:26997-27016 MS.gene066865:CDS 25.0%
! AATTGAAGGTTGTGAAAATA+TGG - 16764:26940-26959 MS.gene066865:CDS 25.0%
! ACATTCAACACTAATCTATT+GGG - 16764:27656-27675 MS.gene066865:intron 25.0%
! AGAAAAGAGTTTATAAAGAG+TGG + 16764:27810-27829 MS.gene066865:intergenic 25.0%
! ATATAAGAGTTTATCCATTC+GGG + 16764:27710-27729 MS.gene066865:intergenic 25.0%
! ATTCAATAAGTTTCCTTTCA+AGG + 16764:27030-27049 MS.gene066865:intergenic 25.0%
! ATTTGCTCATTTCATCATAT+TGG + 16764:28906-28925 MS.gene066865:intergenic 25.0%
! GATATAAGAGTTTATCCATT+CGG + 16764:27711-27730 MS.gene066865:intergenic 25.0%
! TAATACAAAATTACAAGCAG+AGG - 16764:26996-27015 MS.gene066865:CDS 25.0%
! TATTGAATTGAATAGACTGA+AGG - 16764:27042-27061 MS.gene066865:CDS 25.0%
!! TATGGATTATATCACTGAAA+AGG - 16764:26958-26977 MS.gene066865:CDS 25.0%
!!! TGCTTGATAATTTTTTCTAC+AGG - 16764:28150-28169 MS.gene066865:intron 25.0%
AAATGAGATGGAAAGTAAGA+AGG - 16764:27237-27256 MS.gene066865:CDS 30.0%
AATACAACCAGAAACTATGT+TGG - 16764:27915-27934 MS.gene066865:intron 30.0%
AATCAAATCTATCAAAGCCA+AGG - 16764:28674-28693 MS.gene066865:CDS 30.0%
ATGCTAAAAGGACTAACAAA+AGG + 16764:29065-29084 MS.gene066865:intergenic 30.0%
ATGTTCATTAATATGGGACT+TGG - 16764:27538-27557 MS.gene066865:CDS 30.0%
ATTGAATTGCCACAAATTGA+AGG - 16764:26926-26945 MS.gene066865:CDS 30.0%
CACATTCAACACTAATCTAT+TGG - 16764:27655-27674 MS.gene066865:intron 30.0%
GAAAGTAAGAAGGATATGAA+TGG - 16764:27247-27266 MS.gene066865:CDS 30.0%
GAATTCAACTATTACATGCT+TGG - 16764:28855-28874 MS.gene066865:CDS 30.0%
GAGCAAATCATATGAAAATG+TGG - 16764:28918-28937 MS.gene066865:intron 30.0%
GGTTTCTCTAGATTCATAAA+TGG + 16764:28721-28740 MS.gene066865:intergenic 30.0%
TACCATATTAAAAGCAGACT+AGG + 16764:27609-27628 MS.gene066865:intergenic 30.0%
TGATCCTGAAATATGCTAAA+AGG + 16764:29077-29096 MS.gene066865:intergenic 30.0%
TGCTCACAAAACAATCATAT+TGG + 16764:27870-27889 MS.gene066865:intergenic 30.0%
TTATGCTAGTGATGTAATAC+AGG - 16764:26788-26807 MS.gene066865:CDS 30.0%
! AACAAGTTGAAATATTGCAG+AGG - 16764:28239-28258 MS.gene066865:intron 30.0%
! AGATTAGTGTTGAATGTGAA+AGG + 16764:27653-27672 MS.gene066865:intergenic 30.0%
! CACTTATTTCTTCTTGTATC+TGG + 16764:28189-28208 MS.gene066865:intergenic 30.0%
! GATTAGTGTTGAATGTGAAA+GGG + 16764:27652-27671 MS.gene066865:intergenic 30.0%
! TAGTCCTTTTAGCATATTTC+AGG - 16764:29070-29089 MS.gene066865:CDS 30.0%
! TTTTCACAACCTTCAATTTG+TGG + 16764:26938-26957 MS.gene066865:intergenic 30.0%
!! AACCTAGTCTGCTTTTAATA+TGG - 16764:27604-27623 MS.gene066865:CDS 30.0%
!! AGATAGAGCATTACAAGTTT+TGG - 16764:27375-27394 MS.gene066865:CDS 30.0%
!! GATTTTGAGGAAATGGAAAA+TGG - 16764:26759-26778 MS.gene066865:CDS 30.0%
!! TCTGCTTTTAATATGGTACT+TGG - 16764:27611-27630 MS.gene066865:CDS 30.0%
!! TGAACATGATTTTGAGGAAA+TGG - 16764:26752-26771 MS.gene066865:CDS 30.0%
!!! CACCCAATGATTTTTTTTGT+AGG + 16764:27953-27972 MS.gene066865:intergenic 30.0%
!!! TACAAGTTTTGGAAATGAAG+AGG - 16764:27386-27405 MS.gene066865:CDS 30.0%
AACGAGAAGAATTTGCGAAA+GGG - 16764:28595-28614 MS.gene066865:CDS 35.0%
AAGAAACAATTGCTTCCCTT+CGG - 16764:27134-27153 MS.gene066865:CDS 35.0%
AAGAGCTTGAATTGGAAAAG+AGG - 16764:27416-27435 MS.gene066865:CDS 35.0%
ACTACTAGAAGAACTTTCGA+GGG + 16764:28506-28525 MS.gene066865:intergenic 35.0%
ACTAGCAAGAAACAAATCAG+AGG - 16764:27465-27484 MS.gene066865:CDS 35.0%
AGAGCTTGAATTGGAAAAGA+GGG - 16764:27417-27436 MS.gene066865:CDS 35.0%
AGCAATAGCAAGGATCAAAT+TGG - 16764:28985-29004 MS.gene066865:intron 35.0%
AGCATATGAAAAGCCATCTT+TGG + 16764:28297-28316 MS.gene066865:intergenic 35.0%
CAATTCTTTGCCTGAAATCT+TGG + 16764:28383-28402 MS.gene066865:intergenic 35.0%
CTTGGAGTTGAAGAAACAAA+AGG - 16764:28873-28892 MS.gene066865:CDS 35.0%
GAATAAAGGTAGCAATAGCA+AGG - 16764:28975-28994 MS.gene066865:intron 35.0%
GACGAATTAGCGAAGTATAA+CGG - 16764:27313-27332 MS.gene066865:CDS 35.0%
GAGAAACAAAGAGCTTGAAT+TGG - 16764:27408-27427 MS.gene066865:CDS 35.0%
GATTCCTCTTCAGAGAATAA+AGG - 16764:28961-28980 MS.gene066865:intron 35.0%
TGTTATGCAAGAAGAAGTTG+AGG - 16764:28798-28817 MS.gene066865:CDS 35.0%
TTCCCAACTGAAAATGCAAT+TGG - 16764:27081-27100 MS.gene066865:CDS 35.0%
! AGACTCTGAACATGATTTTG+AGG - 16764:26746-26765 MS.gene066865:CDS 35.0%
! CTCCAATTGCATTTTCAGTT+GGG + 16764:27086-27105 MS.gene066865:intergenic 35.0%
! GATGGCTTTTCATATGCTTA+AGG - 16764:28299-28318 MS.gene066865:intron 35.0%
! TCTCCAATTGCATTTTCAGT+TGG + 16764:27087-27106 MS.gene066865:intergenic 35.0%
! TGTTTTGTGAGCAAAGTGAA+AGG - 16764:27877-27896 MS.gene066865:intron 35.0%
!!! AATTTTGGTTTCGAACCGAA+GGG + 16764:27152-27171 MS.gene066865:intergenic 35.0%
!!! TAATTTTGGTTTCGAACCGA+AGG + 16764:27153-27172 MS.gene066865:intergenic 35.0%
AAGAGCTTGTGTACCAAAGA+TGG - 16764:28281-28300 MS.gene066865:intron 40.0%
ACTGGCACATCTAATCTAGT+CGG + 16764:27786-27805 MS.gene066865:intergenic 40.0%
AGAGGGAAATAAGCCTTGAA+AGG - 16764:27014-27033 MS.gene066865:CDS 40.0%
CAACGAGAAGAATTTGCGAA+AGG - 16764:28594-28613 MS.gene066865:CDS 40.0%
CCATGAACCAACATAGTTTC+TGG + 16764:27925-27944 MS.gene066865:intergenic 40.0%
GAATTGGAAAAGAGGGAAGT+AGG - 16764:27424-27443 MS.gene066865:CDS 40.0%
GACACTCTCTTCAATCTTGT+CGG + 16764:28745-28764 MS.gene066865:intergenic 40.0%
GACTACTAGAAGAACTTTCG+AGG + 16764:28507-28526 MS.gene066865:intergenic 40.0%
GAGTTTATAAAGAGTGGCAC+TGG + 16764:27804-27823 MS.gene066865:intergenic 40.0%
TCAACATCTGATCGTTGACA+TGG + 16764:27276-27295 MS.gene066865:intergenic 40.0%
TGATAGGATTAAAAGCCTGC+TGG - 16764:29017-29036 MS.gene066865:CDS 40.0%
TGTCTCTTCGAATGCTACAT+TGG - 16764:28453-28472 MS.gene066865:CDS 40.0%
! ACGTAGGTTTTCAATGTCGA+TGG - 16764:28648-28667 MS.gene066865:CDS 40.0%
! GCTACCTTTATTCTCTGAAG+AGG + 16764:28968-28987 MS.gene066865:intergenic 40.0%
!! CCAGAAACTATGTTGGTTCA+TGG - 16764:27922-27941 MS.gene066865:intron 40.0%
!! GAGAGTGTCTTTCAGTGATT+CGG - 16764:28756-28775 MS.gene066865:CDS 40.0%
!! TCACTTCTTTTGCTTGCTGA+AGG + 16764:26720-26739 MS.gene066865:intergenic 40.0%
AAGCCAAGGAAAAGTTGTGC+TGG - 16764:28688-28707 MS.gene066865:CDS 45.0%
AATCTATTGGGTTTAGCGCG+TGG - 16764:27668-27687 MS.gene066865:intron 45.0%
ACTAGAAGAACTTTCGAGGG+TGG + 16764:28503-28522 MS.gene066865:intergenic 45.0%
ACTCTCTTCAATCTTGTCGG+TGG + 16764:28742-28761 MS.gene066865:intergenic 45.0%
CAAGACTGCACCAAGATTTC+AGG - 16764:28370-28389 MS.gene066865:CDS 45.0%
GAAAACCTACGTATCAGTCC+AGG + 16764:28640-28659 MS.gene066865:intergenic 45.0%
GAGAAGAATTTGCGAAAGGG+TGG - 16764:28598-28617 MS.gene066865:CDS 45.0%
GATTTCTTGGATGACCCGAA+TGG - 16764:27693-27712 MS.gene066865:intron 45.0%
GCAGAGGAACAGATTTGACA+TGG - 16764:28255-28274 MS.gene066865:intron 45.0%
! TTAGCGCGTGGTTGATTTCT+TGG - 16764:27680-27699 MS.gene066865:intron 45.0%
!! GTATAACGGTGCTACTGCTA+TGG - 16764:27327-27346 MS.gene066865:CDS 45.0%
!!! TATTTTTTCTTTAATAATTT+TGG + 16764:27167-27186 MS.gene066865:intergenic 5.0%
CCAAGGAAAAGTTGTGCTGG+TGG - 16764:28691-28710 MS.gene066865:CDS 50.0%
GGAAATAGACCAAGCAGACC+TGG - 16764:28619-28638 MS.gene066865:CDS 50.0%
! CCACCAGCACAACTTTTCCT+TGG + 16764:28694-28713 MS.gene066865:intergenic 50.0%
! CTTGGTGCAGTCTTGTCTTG+TGG + 16764:28365-28384 MS.gene066865:intergenic 50.0%
ATACTAGCAGCTGCACCAGC+AGG + 16764:29035-29054 MS.gene066865:intergenic 55.0%
CGTATCAGTCCAGGTCTGCT+TGG + 16764:28631-28650 MS.gene066865:intergenic 55.0%
GCAGACCTGGACTGATACGT+AGG - 16764:28632-28651 MS.gene066865:CDS 55.0%


Chromosome Type Strat End Strand Name
16764 gene 26714 29101 26714 ID=MS.gene066865
16764 mRNA 26714 29101 26714 ID=MS.gene066865.t1;Parent=MS.gene066865
16764 exon 29006 29101 29006 ID=MS.gene066865.t1.exon1;Parent=MS.gene066865.t1
16764 CDS 29006 29101 29006 ID=cds.MS.gene066865.t1;Parent=MS.gene066865.t1
16764 exon 28329 28913 28329 ID=MS.gene066865.t1.exon2;Parent=MS.gene066865.t1
16764 CDS 28329 28913 28329 ID=cds.MS.gene066865.t1;Parent=MS.gene066865.t1
16764 exon 26714 27643 26714 ID=MS.gene066865.t1.exon3;Parent=MS.gene066865.t1
16764 CDS 26714 27643 26714 ID=cds.MS.gene066865.t1;Parent=MS.gene066865.t1
Gene Sequence

>MS.gene066865

ATGCCTTCAGCAAGCAAAAGAAGTGATAATTCAGACTCTGAACATGATTTTGAGGAAATGGAAAATGGCATTGCTTATGCTAGTGATGTAATACAGGTACCTTTTATGTGTTATTTTTTGTTTTTTTTGTGTGATAAGTTCAGTAGAAATGTATCCCAAAAAATTGATTTGTCTTTAATTTGCTACAGAATGAAGAAAAGAAGAAGATTAAAATTGAATTGCCACAAATTGAAGGTTGTGAAAATATGGATTATATCACTGAAAAGGAGTTGCTGCAGAATTTAATACAAAATTACAAGCAGAGGGAAATAAGCCTTGAAAGGAAACTTATTGAATTGAATAGACTGAAGGAAGAGAAATCAGTCATTTCCCAACTGAAAATGCAATTGGAGAAAAAGAGAGCAAAATTAAAATCTCTTGAAGAAACAATTGCTTCCCTTCGGTTCGAAACCAAAATTATTAAAGAAAAAATAGAAGAGGATATCATGTGTAAAATGCAGTTAGAGATTGCAAATAAAATTATAAATGAGATGGAAAGTAAGAAGGATATGAATGGAAGCCATGTCAACGATCAGATGTTGACGCTTCAACAACGAGTTGACGAATTAGCGAAGTATAACGGTGCTACTGCTATGGTGAATAAGAAACATGAACATGTTGAAGATAGAGCATTACAAGTTTTGGAAATGAAGAGGAGAAACAAAGAGCTTGAATTGGAAAAGAGGGAAGTAGGAACTAAGTTAGCTATTGCACTAGCAAGAAACAAATCAGAGGTAAGAACACTGCATCATAGAAGAAATTGATTTTTTTAGTTCATTATTCTTATGTTCATTAATATGGGACTTGGACTATTCTGAATATACCATCTTGTATTATTAAAATTATGTTTAAACCTAGTCTGCTTTTAATATGGTACTTGGATTTTGTTCGATATACCCTTTCACATTCAACACTAATCTATTGGGTTTAGCGCGTGGTTGATTTCTTGGATGACCCGAATGGATAAACTCTTATATCAAACTAGATTTTTTATATTATATTGTATATAATTCTAACTCTTTGTTACAAACCGACTAGATTAGATGTGCCAGTGCCACTCTTTATAAACTCTTTTCTGACATTATCGATTTTCGATGTGAGATTTGAATTTTTTCCAATATGATTGTTTTGTGAGCAAAGTGAAAGGAGTTTATTATAATACAATACAACCAGAAACTATGTTGGTTCATGGTTAATCCTACAAAAAAAATCATTGGGTGAAAGAACTGTAACTGTTCTCTACCTATAATTAATCAACATTACTTAAGTTATGGATACTTCAAACTAAATTTTGTTAAAATAATAGGTAGTAGGTAATAGTTATTTTGTTCTTTGTTGTCCTCTACCTTAAATTTATTGTTTAAGGCTAGTTTAAGCACTAAGCAATAAGTTTCTTATGCTTGATAATTTTTTCTACAGGAAACAAAAGTGATCCAGATACAAGAAGAAATAAGTGATTTACAACATGTTCATGAACAACTTTCAGAACAAGTTGAAATATTGCAGAGGAACAGATTTGACATGGTACAAGAGCTTGTGTACCAAAGATGGCTTTTCATATGCTTAAGGTATGAAGTTAACAATCATAATAAAAAGCATAATAGAAAGGCCACAAGACAAGACTGCACCAAGATTTCAGGCAAAGAATTGTTGTATGATGAGAAAACTCATTCATTCACATCTGATTCTGCACATGACACTGTCTCTTCGAATGCTACATTGGATGATTTTGATGAAATCGAAACTACCACCCTCGAAAGTTCTTCTAGTAGTCAAAGTAACAACAGTAGCAATTCCAGTTTGCTTAATAAATTGAAAAGATGGAAGAAATCAAAAGATTACAACGAGAAGAATTTGCGAAAGGGTGGAAATAGACCAAGCAGACCTGGACTGATACGTAGGTTTTCAATGTCGATGGTTGAATCAAATCTATCAAAGCCAAGGAAAAGTTGTGCTGGTGGTGATCCATTTATGAATCTAGAGAAACCACCGACAAGATTGAAGAGAGTGTCTTTCAGTGATTCGGTTAAAAAATTGACTTATCATGTTATGCAAGAAGAAGTTGAGGATGTAACTGATGAAAATGAAACTAACTCTCAGCTGAATTCAACTATTACATGCTTGGAGTTGAAGAAACAAAAGGAAGAAGACCAATATGATGAAATGAGCAAATCATATGAAAATGTGGTTTGCAACTATGAAAAAGTTGATTCCTCTTCAGAGAATAAAGGTAGCAATAGCAAGGATCAAATTGGTTATTCAGATGATAGGATTAAAAGCCTGCTGGTGCAGCTGCTAGTATATTTTTTCCTTTTGTTAGTCCTTTTAGCATATTTCAGGATCAAATAA

Protein sequence

>MS.gene066865.t1

MPSASKRSDNSDSEHDFEEMENGIAYASDVIQNEEKKKIKIELPQIEGCENMDYITEKELLQNLIQNYKQREISLERKLIELNRLKEEKSVISQLKMQLEKKRAKLKSLEETIASLRFETKIIKEKIEEDIMCKMQLEIANKIINEMESKKDMNGSHVNDQMLTLQQRVDELAKYNGATAMVNKKHEHVEDRALQVLEMKRRNKELELEKREVGTKLAIALARNKSEETKVIQIQEEISDLQHVHEQLSEQVEILQRNRFDMVQELVYQRWLFICLRYEVNNHNKKHNRKATRQDCTKISGKELLYDEKTHSFTSDSAHDTVSSNATLDDFDEIETTTLESSSSSQSNNSSNSSLLNKLKRWKKSKDYNEKNLRKGGNRPSRPGLIRRFSMSMVESNLSKPRKSCAGGDPFMNLEKPPTRLKRVSFSDSVKKLTYHVMQEEVEDVTDENETNSQLNSTITCLELKKQKEEDQYDEMSKSYENVVCNYEKVDSSSENKGSNSKDQIGYSDDRIKSLLVQLLVYFFLLLVLLAYFRIK