AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066866


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066866.t1 MTR_0170s0030 89.555 517 52 2 4 518 31 547 0.0 879
MS.gene066866.t1 MTR_5g053270 36.254 331 189 4 56 367 126 453 2.79e-41 159
MS.gene066866.t1 MTR_1g093850 31.214 346 199 6 56 367 133 473 1.90e-32 133
MS.gene066866.t1 MTR_2g100630 23.098 368 222 13 39 367 113 458 1.53e-14 76.6
MS.gene066866.t1 MTR_2g100630 23.098 368 222 13 39 367 72 417 2.02e-14 76.3
MS.gene066866.t1 MTR_8g014900 22.630 327 233 5 61 367 144 470 3.47e-14 75.9
MS.gene066866.t1 MTR_1g016290 40.426 94 51 1 194 282 207 300 2.49e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066866.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066866.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066866.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066866.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066866.t1 AT3G25690 30.617 454 264 9 34 448 117 558 2.29e-41 160
MS.gene066866.t1 AT3G25690 29.973 367 214 8 121 448 53 415 1.57e-30 127
MS.gene066866.t1 AT1G52080 26.837 313 214 6 50 355 133 437 2.74e-15 79.3
MS.gene066866.t1 AT1G52080 26.837 313 214 6 50 355 133 437 2.74e-15 79.3

Find 0 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATTATAAATGAGA+TGG - 16767:1718-1737 MS.gene066866:CDS 10.0%
!!! ATAATTTTAATAATACAAGA+TGG + 16767:2072-2091 MS.gene066866:intergenic 10.0%
!!! CTAAATTTTGTTAAAATAAT+AGG - 16767:2530-2549 MS.gene066866:intron 10.0%
!! AACAAAAAATAACACATAAA+AGG + 16767:1309-1328 MS.gene066866:intergenic 15.0%
!! ATTCTTATGTTCATTAATAT+GGG - 16767:2025-2044 MS.gene066866:CDS 15.0%
!! TACCTTAAATTTATTGTTTA+AGG - 16767:2589-2608 MS.gene066866:intron 15.0%
!! TATTAAAGAAAAAATAGAAG+AGG - 16767:1664-1683 MS.gene066866:CDS 15.0%
!! TATTCTTATGTTCATTAATA+TGG - 16767:2024-2043 MS.gene066866:CDS 15.0%
!!! AAGTAATGTTGATTAATTAT+AGG + 16767:2491-2510 MS.gene066866:intergenic 15.0%
!!! TAAAGACAAATCAATTTTTT+GGG + 16767:1364-1383 MS.gene066866:intergenic 15.0%
!!! TAATAAAAAGCATAATAGAA+AGG - 16767:2832-2851 MS.gene066866:CDS 15.0%
!!! TTAAAGACAAATCAATTTTT+TGG + 16767:1365-1384 MS.gene066866:intergenic 15.0%
!! AATCAACATTACTTAAGTTA+TGG - 16767:2497-2516 MS.gene066866:intron 20.0%
!! AATCCTACAAAAAAAATCAT+TGG - 16767:2440-2459 MS.gene066866:intron 20.0%
!! AGCCTTAAACAATAAATTTA+AGG + 16767:2594-2613 MS.gene066866:intergenic 20.0%
!! ATCCTACAAAAAAAATCATT+GGG - 16767:2441-2460 MS.gene066866:intron 20.0%
!! TGCTTAATAAATTGAAAAGA+TGG - 16767:3047-3066 MS.gene066866:CDS 20.0%
!! TTGTTAAAATAATAGGTAGT+AGG - 16767:2537-2556 MS.gene066866:intron 20.0%
!! TTTCAATTTATTAAGCAAAC+TGG + 16767:3044-3063 MS.gene066866:intergenic 20.0%
!!! AAACAATAAATTTAAGGTAG+AGG + 16767:2588-2607 MS.gene066866:intergenic 20.0%
! AAATTGGTTATTCAGATGAT+AGG - 16767:3494-3513 MS.gene066866:intron 25.0%
! AATACAAAATTACAAGCAGA+GGG - 16767:1490-1509 MS.gene066866:CDS 25.0%
! AATTGAAGGTTGTGAAAATA+TGG - 16767:1433-1452 MS.gene066866:CDS 25.0%
! ACATTCAACACTAATCTATT+GGG - 16767:2149-2168 MS.gene066866:intron 25.0%
! AGAAAAGAGTTTATAAAGAG+TGG + 16767:2303-2322 MS.gene066866:intergenic 25.0%
! ATATAAGAGTTTATCCATTC+GGG + 16767:2203-2222 MS.gene066866:intergenic 25.0%
! ATTCAATAAGTTTCCTTTCA+AGG + 16767:1523-1542 MS.gene066866:intergenic 25.0%
! ATTTGCTCATTTCATCATAT+TGG + 16767:3399-3418 MS.gene066866:intergenic 25.0%
! GATATAAGAGTTTATCCATT+CGG + 16767:2204-2223 MS.gene066866:intergenic 25.0%
! TAATACAAAATTACAAGCAG+AGG - 16767:1489-1508 MS.gene066866:CDS 25.0%
! TATTGAATTGAATAGACTGA+AGG - 16767:1535-1554 MS.gene066866:CDS 25.0%
!! TATGGATTATATCACTGAAA+AGG - 16767:1451-1470 MS.gene066866:CDS 25.0%
!!! TGCTTGATAATTTTTTCTAC+AGG - 16767:2643-2662 MS.gene066866:intron 25.0%
AAATGAGATGGAAAGTAAGA+AGG - 16767:1730-1749 MS.gene066866:CDS 30.0%
AATACAACCAGAAACTATGT+TGG - 16767:2408-2427 MS.gene066866:intron 30.0%
AATCAAATCTATCAAAGCCA+AGG - 16767:3167-3186 MS.gene066866:CDS 30.0%
ATGCTAAAAGGACTAACAAA+AGG + 16767:3558-3577 MS.gene066866:intergenic 30.0%
ATGTTCATTAATATGGGACT+TGG - 16767:2031-2050 MS.gene066866:CDS 30.0%
ATTGAATTGCCACAAATTGA+AGG - 16767:1419-1438 MS.gene066866:CDS 30.0%
CACATTCAACACTAATCTAT+TGG - 16767:2148-2167 MS.gene066866:intron 30.0%
GAAAGTAAGAAGGATATGAA+TGG - 16767:1740-1759 MS.gene066866:CDS 30.0%
GAATTCAACTATTACATGCT+TGG - 16767:3348-3367 MS.gene066866:CDS 30.0%
GAGCAAATCATATGAAAATG+TGG - 16767:3411-3430 MS.gene066866:intron 30.0%
GGTTTCTCTAGATTCATAAA+TGG + 16767:3214-3233 MS.gene066866:intergenic 30.0%
TACCATATTAAAAGCAGACT+AGG + 16767:2102-2121 MS.gene066866:intergenic 30.0%
TGATCCTGAAATATGCTAAA+AGG + 16767:3570-3589 MS.gene066866:intergenic 30.0%
TGCTCACAAAACAATCATAT+TGG + 16767:2363-2382 MS.gene066866:intergenic 30.0%
TTATGCTAGTGATGTAATAC+AGG - 16767:1281-1300 MS.gene066866:CDS 30.0%
! AACAAGTTGAAATATTGCAG+AGG - 16767:2732-2751 MS.gene066866:intron 30.0%
! AGATTAGTGTTGAATGTGAA+AGG + 16767:2146-2165 MS.gene066866:intergenic 30.0%
! CACTTATTTCTTCTTGTATC+TGG + 16767:2682-2701 MS.gene066866:intergenic 30.0%
! GATTAGTGTTGAATGTGAAA+GGG + 16767:2145-2164 MS.gene066866:intergenic 30.0%
! TAGTCCTTTTAGCATATTTC+AGG - 16767:3563-3582 MS.gene066866:CDS 30.0%
! TTTTCACAACCTTCAATTTG+TGG + 16767:1431-1450 MS.gene066866:intergenic 30.0%
!! AACCTAGTCTGCTTTTAATA+TGG - 16767:2097-2116 MS.gene066866:CDS 30.0%
!! AGATAGAGCATTACAAGTTT+TGG - 16767:1868-1887 MS.gene066866:CDS 30.0%
!! GATTTTGAGGAAATGGAAAA+TGG - 16767:1252-1271 MS.gene066866:CDS 30.0%
!! TCTGCTTTTAATATGGTACT+TGG - 16767:2104-2123 MS.gene066866:CDS 30.0%
!! TGAACATGATTTTGAGGAAA+TGG - 16767:1245-1264 MS.gene066866:CDS 30.0%
!!! CACCCAATGATTTTTTTTGT+AGG + 16767:2446-2465 MS.gene066866:intergenic 30.0%
!!! TACAAGTTTTGGAAATGAAG+AGG - 16767:1879-1898 MS.gene066866:CDS 30.0%
AACGAGAAGAATTTGCGAAA+GGG - 16767:3088-3107 MS.gene066866:CDS 35.0%
AAGAAACAATTGCTTCCCTT+CGG - 16767:1627-1646 MS.gene066866:CDS 35.0%
AAGAGCTTGAATTGGAAAAG+AGG - 16767:1909-1928 MS.gene066866:CDS 35.0%
ACTACTAGAAGAACTTTCGA+GGG + 16767:2999-3018 MS.gene066866:intergenic 35.0%
ACTAGCAAGAAACAAATCAG+AGG - 16767:1958-1977 MS.gene066866:CDS 35.0%
AGAGCTTGAATTGGAAAAGA+GGG - 16767:1910-1929 MS.gene066866:CDS 35.0%
AGCAATAGCAAGGATCAAAT+TGG - 16767:3478-3497 MS.gene066866:intron 35.0%
AGCATATGAAAAGCCATCTT+TGG + 16767:2790-2809 MS.gene066866:intergenic 35.0%
CAATTCTTTGCCTGAAATCT+TGG + 16767:2876-2895 MS.gene066866:intergenic 35.0%
CTTGGAGTTGAAGAAACAAA+AGG - 16767:3366-3385 MS.gene066866:CDS 35.0%
GAATAAAGGTAGCAATAGCA+AGG - 16767:3468-3487 MS.gene066866:intron 35.0%
GACGAATTAGCGAAGTATAA+CGG - 16767:1806-1825 MS.gene066866:CDS 35.0%
GAGAAACAAAGAGCTTGAAT+TGG - 16767:1901-1920 MS.gene066866:CDS 35.0%
GATTCCTCTTCAGAGAATAA+AGG - 16767:3454-3473 MS.gene066866:intron 35.0%
TGTTATGCAAGAAGAAGTTG+AGG - 16767:3291-3310 MS.gene066866:CDS 35.0%
TTCCCAACTGAAAATGCAAT+TGG - 16767:1574-1593 MS.gene066866:CDS 35.0%
! AGACTCTGAACATGATTTTG+AGG - 16767:1239-1258 MS.gene066866:CDS 35.0%
! CTCCAATTGCATTTTCAGTT+GGG + 16767:1579-1598 MS.gene066866:intergenic 35.0%
! GATGGCTTTTCATATGCTTA+AGG - 16767:2792-2811 MS.gene066866:intron 35.0%
! TCTCCAATTGCATTTTCAGT+TGG + 16767:1580-1599 MS.gene066866:intergenic 35.0%
! TGTTTTGTGAGCAAAGTGAA+AGG - 16767:2370-2389 MS.gene066866:intron 35.0%
!!! AATTTTGGTTTCGAACCGAA+GGG + 16767:1645-1664 MS.gene066866:intergenic 35.0%
!!! TAATTTTGGTTTCGAACCGA+AGG + 16767:1646-1665 MS.gene066866:intergenic 35.0%
AAGAGCTTGTGTACCAAAGA+TGG - 16767:2774-2793 MS.gene066866:intron 40.0%
ACTGGCACATCTAATCTAGT+CGG + 16767:2279-2298 MS.gene066866:intergenic 40.0%
AGAGGGAAATAAGCCTTGAA+AGG - 16767:1507-1526 MS.gene066866:CDS 40.0%
CAACGAGAAGAATTTGCGAA+AGG - 16767:3087-3106 MS.gene066866:CDS 40.0%
CCATGAACCAACATAGTTTC+TGG + 16767:2418-2437 MS.gene066866:intergenic 40.0%
GAATTGGAAAAGAGGGAAGT+AGG - 16767:1917-1936 MS.gene066866:CDS 40.0%
GACACTCTCTTCAATCTTGT+CGG + 16767:3238-3257 MS.gene066866:intergenic 40.0%
GACTACTAGAAGAACTTTCG+AGG + 16767:3000-3019 MS.gene066866:intergenic 40.0%
GAGTTTATAAAGAGTGGCAC+TGG + 16767:2297-2316 MS.gene066866:intergenic 40.0%
TCAACATCTGATCGTTGACA+TGG + 16767:1769-1788 MS.gene066866:intergenic 40.0%
TGATAGGATTAAAAGCCTGC+TGG - 16767:3510-3529 MS.gene066866:CDS 40.0%
TGTCTCTTCGAATGCTACAT+TGG - 16767:2946-2965 MS.gene066866:CDS 40.0%
! ACGTAGGTTTTCAATGTCGA+TGG - 16767:3141-3160 MS.gene066866:CDS 40.0%
! GCTACCTTTATTCTCTGAAG+AGG + 16767:3461-3480 MS.gene066866:intergenic 40.0%
!! CCAGAAACTATGTTGGTTCA+TGG - 16767:2415-2434 MS.gene066866:intron 40.0%
!! GAGAGTGTCTTTCAGTGATT+CGG - 16767:3249-3268 MS.gene066866:CDS 40.0%
!! TCACTTCTTTTGCTTGCTGA+AGG + 16767:1213-1232 MS.gene066866:intergenic 40.0%
AAGCCAAGGAAAAGTTGTGC+TGG - 16767:3181-3200 MS.gene066866:CDS 45.0%
AATCTATTGGGTTTAGCGCG+TGG - 16767:2161-2180 MS.gene066866:intron 45.0%
ACTAGAAGAACTTTCGAGGG+TGG + 16767:2996-3015 MS.gene066866:intergenic 45.0%
ACTCTCTTCAATCTTGTCGG+TGG + 16767:3235-3254 MS.gene066866:intergenic 45.0%
CAAGACTGCACCAAGATTTC+AGG - 16767:2863-2882 MS.gene066866:CDS 45.0%
GAAAACCTACGTATCAGTCC+AGG + 16767:3133-3152 MS.gene066866:intergenic 45.0%
GAGAAGAATTTGCGAAAGGG+TGG - 16767:3091-3110 MS.gene066866:CDS 45.0%
GATTTCTTGGATGACCCGAA+TGG - 16767:2186-2205 MS.gene066866:intron 45.0%
GCAGAGGAACAGATTTGACA+TGG - 16767:2748-2767 MS.gene066866:intron 45.0%
! TTAGCGCGTGGTTGATTTCT+TGG - 16767:2173-2192 MS.gene066866:intron 45.0%
!! GTATAACGGTGCTACTGCTA+TGG - 16767:1820-1839 MS.gene066866:CDS 45.0%
!!! TATTTTTTCTTTAATAATTT+TGG + 16767:1660-1679 MS.gene066866:intergenic 5.0%
CCAAGGAAAAGTTGTGCTGG+TGG - 16767:3184-3203 MS.gene066866:CDS 50.0%
GGAAATAGACCAAGCAGACC+TGG - 16767:3112-3131 MS.gene066866:CDS 50.0%
! CCACCAGCACAACTTTTCCT+TGG + 16767:3187-3206 MS.gene066866:intergenic 50.0%
! CTTGGTGCAGTCTTGTCTTG+TGG + 16767:2858-2877 MS.gene066866:intergenic 50.0%
ATACTAGCAGCTGCACCAGC+AGG + 16767:3528-3547 MS.gene066866:intergenic 55.0%
CGTATCAGTCCAGGTCTGCT+TGG + 16767:3124-3143 MS.gene066866:intergenic 55.0%
GCAGACCTGGACTGATACGT+AGG - 16767:3125-3144 MS.gene066866:CDS 55.0%


Chromosome Type Strat End Strand Name
16767 gene 1207 3594 1207 ID=MS.gene066866
16767 mRNA 1207 3594 1207 ID=MS.gene066866.t1;Parent=MS.gene066866
16767 exon 3499 3594 3499 ID=MS.gene066866.t1.exon1;Parent=MS.gene066866.t1
16767 CDS 3499 3594 3499 ID=cds.MS.gene066866.t1;Parent=MS.gene066866.t1
16767 exon 2822 3406 2822 ID=MS.gene066866.t1.exon2;Parent=MS.gene066866.t1
16767 CDS 2822 3406 2822 ID=cds.MS.gene066866.t1;Parent=MS.gene066866.t1
16767 exon 1207 2136 1207 ID=MS.gene066866.t1.exon3;Parent=MS.gene066866.t1
16767 CDS 1207 2136 1207 ID=cds.MS.gene066866.t1;Parent=MS.gene066866.t1
Gene Sequence

>MS.gene066866

ATGCCTTCAGCAAGCAAAAGAAGTGATAATTCAGACTCTGAACATGATTTTGAGGAAATGGAAAATGGCATTGCTTATGCTAGTGATGTAATACAGGTACCTTTTATGTGTTATTTTTTGTTTTTTTTGTGTGATAAGTTCAGTAGAAATGTATCCCAAAAAATTGATTTGTCTTTAATTTGCTACAGAATGAAGAAAAGAAGAAGATTAAAATTGAATTGCCACAAATTGAAGGTTGTGAAAATATGGATTATATCACTGAAAAGGAGTTGCTGCAGAATTTAATACAAAATTACAAGCAGAGGGAAATAAGCCTTGAAAGGAAACTTATTGAATTGAATAGACTGAAGGAAGAGAAATCAGTCATTTCCCAACTGAAAATGCAATTGGAGAAAAAGAGAGCAAAATTAAAATCTCTTGAAGAAACAATTGCTTCCCTTCGGTTCGAAACCAAAATTATTAAAGAAAAAATAGAAGAGGATATCATGTGTAAAATGCAGTTAGAGATTGCAAATAAAATTATAAATGAGATGGAAAGTAAGAAGGATATGAATGGAAGCCATGTCAACGATCAGATGTTGACGCTTCAACAACGAGTTGACGAATTAGCGAAGTATAACGGTGCTACTGCTATGGTGAATAAGAAACATGAACATGTTGAAGATAGAGCATTACAAGTTTTGGAAATGAAGAGGAGAAACAAAGAGCTTGAATTGGAAAAGAGGGAAGTAGGAACTAAGTTAGCTATTGCACTAGCAAGAAACAAATCAGAGGTAAGAACACTGCATCATAGAAGAAATTGATTTTTTTAGTTCATTATTCTTATGTTCATTAATATGGGACTTGGACTATTCTGAATATACCATCTTGTATTATTAAAATTATGTTTAAACCTAGTCTGCTTTTAATATGGTACTTGGATTTTGTTCGATATACCCTTTCACATTCAACACTAATCTATTGGGTTTAGCGCGTGGTTGATTTCTTGGATGACCCGAATGGATAAACTCTTATATCAAACTAGATTTTTTATATTATATTGTATATAATTCTAACTCTTTGTTACAAACCGACTAGATTAGATGTGCCAGTGCCACTCTTTATAAACTCTTTTCTGACATTATCGATTTTCGATGTGAGATTTGAATTTTTTCCAATATGATTGTTTTGTGAGCAAAGTGAAAGGAGTTTATTATAATACAATACAACCAGAAACTATGTTGGTTCATGGTTAATCCTACAAAAAAAATCATTGGGTGAAAGAACTGTAACTGTTCTCTACCTATAATTAATCAACATTACTTAAGTTATGGATACTTCAAACTAAATTTTGTTAAAATAATAGGTAGTAGGTAATAGTTATTTTGTTCTTTGTTGTCCTCTACCTTAAATTTATTGTTTAAGGCTAGTTTAAGCACTAAGCAATAAGTTTCTTATGCTTGATAATTTTTTCTACAGGAAACAAAAGTGATCCAGATACAAGAAGAAATAAGTGATTTACAACATGTTCATGAACAACTTTCAGAACAAGTTGAAATATTGCAGAGGAACAGATTTGACATGGTACAAGAGCTTGTGTACCAAAGATGGCTTTTCATATGCTTAAGGTATGAAGTTAACAATCATAATAAAAAGCATAATAGAAAGGCCACAAGACAAGACTGCACCAAGATTTCAGGCAAAGAATTGTTGTATGATGAGAAAACTCATTCATTCACATCTGATTCTGCACATGACACTGTCTCTTCGAATGCTACATTGGATGATTTTGATGAAATCGAAACTACCACCCTCGAAAGTTCTTCTAGTAGTCAAAGTAACAACAGTAGCAATTCCAGTTTGCTTAATAAATTGAAAAGATGGAAGAAATCAAAAGATTACAACGAGAAGAATTTGCGAAAGGGTGGAAATAGACCAAGCAGACCTGGACTGATACGTAGGTTTTCAATGTCGATGGTTGAATCAAATCTATCAAAGCCAAGGAAAAGTTGTGCTGGTGGTGATCCATTTATGAATCTAGAGAAACCACCGACAAGATTGAAGAGAGTGTCTTTCAGTGATTCGGTTAAAAAATTGACTTATCATGTTATGCAAGAAGAAGTTGAGGATGTAACTGATGAAAATGAAACTAACTCTCAGCTGAATTCAACTATTACATGCTTGGAGTTGAAGAAACAAAAGGAAGAAGACCAATATGATGAAATGAGCAAATCATATGAAAATGTGGTTTGCAACTATGAAAAAGTTGATTCCTCTTCAGAGAATAAAGGTAGCAATAGCAAGGATCAAATTGGTTATTCAGATGATAGGATTAAAAGCCTGCTGGTGCAGCTGCTAGTATATTTTTTCCTTTTGTTAGTCCTTTTAGCATATTTCAGGATCAAATAA

Protein sequence

>MS.gene066866.t1

MPSASKRSDNSDSEHDFEEMENGIAYASDVIQNEEKKKIKIELPQIEGCENMDYITEKELLQNLIQNYKQREISLERKLIELNRLKEEKSVISQLKMQLEKKRAKLKSLEETIASLRFETKIIKEKIEEDIMCKMQLEIANKIINEMESKKDMNGSHVNDQMLTLQQRVDELAKYNGATAMVNKKHEHVEDRALQVLEMKRRNKELELEKREVGTKLAIALARNKSEETKVIQIQEEISDLQHVHEQLSEQVEILQRNRFDMVQELVYQRWLFICLRYEVNNHNKKHNRKATRQDCTKISGKELLYDEKTHSFTSDSAHDTVSSNATLDDFDEIETTTLESSSSSQSNNSSNSSLLNKLKRWKKSKDYNEKNLRKGGNRPSRPGLIRRFSMSMVESNLSKPRKSCAGGDPFMNLEKPPTRLKRVSFSDSVKKLTYHVMQEEVEDVTDENETNSQLNSTITCLELKKQKEEDQYDEMSKSYENVVCNYEKVDSSSENKGSNSKDQIGYSDDRIKSLLVQLLVYFFLLLVLLAYFRIK