AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066868


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066868.t1 MTR_0170s0030 89.555 517 52 2 4 518 31 547 0.0 879
MS.gene066868.t1 MTR_5g053270 36.254 331 189 4 56 367 126 453 2.79e-41 159
MS.gene066868.t1 MTR_1g093850 31.214 346 199 6 56 367 133 473 1.90e-32 133
MS.gene066868.t1 MTR_2g100630 23.098 368 222 13 39 367 113 458 1.53e-14 76.6
MS.gene066868.t1 MTR_2g100630 23.098 368 222 13 39 367 72 417 2.02e-14 76.3
MS.gene066868.t1 MTR_8g014900 22.630 327 233 5 61 367 144 470 3.47e-14 75.9
MS.gene066868.t1 MTR_1g016290 40.426 94 51 1 194 282 207 300 2.49e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066868.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066868.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066868.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066868.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.92e-41 160
MS.gene066868.t1 AT3G25690 30.617 454 264 9 34 448 117 558 2.29e-41 160
MS.gene066868.t1 AT3G25690 29.973 367 214 8 121 448 53 415 1.57e-30 127
MS.gene066868.t1 AT1G52080 26.837 313 214 6 50 355 133 437 2.74e-15 79.3
MS.gene066868.t1 AT1G52080 26.837 313 214 6 50 355 133 437 2.74e-15 79.3

Find 0 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATTATAAATGAGA+TGG - 16769:1503-1522 MS.gene066868:CDS 10.0%
!!! ATAATTTTAATAATACAAGA+TGG + 16769:1857-1876 MS.gene066868:intergenic 10.0%
!!! CTAAATTTTGTTAAAATAAT+AGG - 16769:2315-2334 MS.gene066868:intron 10.0%
!! AACAAAAAATAACACATAAA+AGG + 16769:1094-1113 MS.gene066868:intergenic 15.0%
!! ATTCTTATGTTCATTAATAT+GGG - 16769:1810-1829 MS.gene066868:CDS 15.0%
!! TACCTTAAATTTATTGTTTA+AGG - 16769:2374-2393 MS.gene066868:intron 15.0%
!! TATTAAAGAAAAAATAGAAG+AGG - 16769:1449-1468 MS.gene066868:CDS 15.0%
!! TATTCTTATGTTCATTAATA+TGG - 16769:1809-1828 MS.gene066868:CDS 15.0%
!!! AAGTAATGTTGATTAATTAT+AGG + 16769:2276-2295 MS.gene066868:intergenic 15.0%
!!! TAAAGACAAATCAATTTTTT+GGG + 16769:1149-1168 MS.gene066868:intergenic 15.0%
!!! TAATAAAAAGCATAATAGAA+AGG - 16769:2617-2636 MS.gene066868:CDS 15.0%
!!! TTAAAGACAAATCAATTTTT+TGG + 16769:1150-1169 MS.gene066868:intergenic 15.0%
!! AATCAACATTACTTAAGTTA+TGG - 16769:2282-2301 MS.gene066868:intron 20.0%
!! AATCCTACAAAAAAAATCAT+TGG - 16769:2225-2244 MS.gene066868:intron 20.0%
!! AGCCTTAAACAATAAATTTA+AGG + 16769:2379-2398 MS.gene066868:intergenic 20.0%
!! ATCCTACAAAAAAAATCATT+GGG - 16769:2226-2245 MS.gene066868:intron 20.0%
!! TGCTTAATAAATTGAAAAGA+TGG - 16769:2832-2851 MS.gene066868:CDS 20.0%
!! TTGTTAAAATAATAGGTAGT+AGG - 16769:2322-2341 MS.gene066868:intron 20.0%
!! TTTCAATTTATTAAGCAAAC+TGG + 16769:2829-2848 MS.gene066868:intergenic 20.0%
!!! AAACAATAAATTTAAGGTAG+AGG + 16769:2373-2392 MS.gene066868:intergenic 20.0%
! AAATTGGTTATTCAGATGAT+AGG - 16769:3279-3298 MS.gene066868:intron 25.0%
! AATACAAAATTACAAGCAGA+GGG - 16769:1275-1294 MS.gene066868:CDS 25.0%
! AATTGAAGGTTGTGAAAATA+TGG - 16769:1218-1237 MS.gene066868:CDS 25.0%
! ACATTCAACACTAATCTATT+GGG - 16769:1934-1953 MS.gene066868:intron 25.0%
! AGAAAAGAGTTTATAAAGAG+TGG + 16769:2088-2107 MS.gene066868:intergenic 25.0%
! ATATAAGAGTTTATCCATTC+GGG + 16769:1988-2007 MS.gene066868:intergenic 25.0%
! ATTCAATAAGTTTCCTTTCA+AGG + 16769:1308-1327 MS.gene066868:intergenic 25.0%
! ATTTGCTCATTTCATCATAT+TGG + 16769:3184-3203 MS.gene066868:intergenic 25.0%
! GATATAAGAGTTTATCCATT+CGG + 16769:1989-2008 MS.gene066868:intergenic 25.0%
! TAATACAAAATTACAAGCAG+AGG - 16769:1274-1293 MS.gene066868:CDS 25.0%
! TATTGAATTGAATAGACTGA+AGG - 16769:1320-1339 MS.gene066868:CDS 25.0%
!! TATGGATTATATCACTGAAA+AGG - 16769:1236-1255 MS.gene066868:CDS 25.0%
!!! TGCTTGATAATTTTTTCTAC+AGG - 16769:2428-2447 MS.gene066868:intron 25.0%
AAATGAGATGGAAAGTAAGA+AGG - 16769:1515-1534 MS.gene066868:CDS 30.0%
AATACAACCAGAAACTATGT+TGG - 16769:2193-2212 MS.gene066868:intron 30.0%
AATCAAATCTATCAAAGCCA+AGG - 16769:2952-2971 MS.gene066868:CDS 30.0%
ATGCTAAAAGGACTAACAAA+AGG + 16769:3343-3362 MS.gene066868:intergenic 30.0%
ATGTTCATTAATATGGGACT+TGG - 16769:1816-1835 MS.gene066868:CDS 30.0%
ATTGAATTGCCACAAATTGA+AGG - 16769:1204-1223 MS.gene066868:CDS 30.0%
CACATTCAACACTAATCTAT+TGG - 16769:1933-1952 MS.gene066868:intron 30.0%
GAAAGTAAGAAGGATATGAA+TGG - 16769:1525-1544 MS.gene066868:CDS 30.0%
GAATTCAACTATTACATGCT+TGG - 16769:3133-3152 MS.gene066868:CDS 30.0%
GAGCAAATCATATGAAAATG+TGG - 16769:3196-3215 MS.gene066868:intron 30.0%
GGTTTCTCTAGATTCATAAA+TGG + 16769:2999-3018 MS.gene066868:intergenic 30.0%
TACCATATTAAAAGCAGACT+AGG + 16769:1887-1906 MS.gene066868:intergenic 30.0%
TGATCCTGAAATATGCTAAA+AGG + 16769:3355-3374 MS.gene066868:intergenic 30.0%
TGCTCACAAAACAATCATAT+TGG + 16769:2148-2167 MS.gene066868:intergenic 30.0%
TTATGCTAGTGATGTAATAC+AGG - 16769:1066-1085 MS.gene066868:CDS 30.0%
! AACAAGTTGAAATATTGCAG+AGG - 16769:2517-2536 MS.gene066868:intron 30.0%
! AGATTAGTGTTGAATGTGAA+AGG + 16769:1931-1950 MS.gene066868:intergenic 30.0%
! CACTTATTTCTTCTTGTATC+TGG + 16769:2467-2486 MS.gene066868:intergenic 30.0%
! GATTAGTGTTGAATGTGAAA+GGG + 16769:1930-1949 MS.gene066868:intergenic 30.0%
! TAGTCCTTTTAGCATATTTC+AGG - 16769:3348-3367 MS.gene066868:CDS 30.0%
! TTTTCACAACCTTCAATTTG+TGG + 16769:1216-1235 MS.gene066868:intergenic 30.0%
!! AACCTAGTCTGCTTTTAATA+TGG - 16769:1882-1901 MS.gene066868:CDS 30.0%
!! AGATAGAGCATTACAAGTTT+TGG - 16769:1653-1672 MS.gene066868:CDS 30.0%
!! GATTTTGAGGAAATGGAAAA+TGG - 16769:1037-1056 MS.gene066868:CDS 30.0%
!! TCTGCTTTTAATATGGTACT+TGG - 16769:1889-1908 MS.gene066868:CDS 30.0%
!! TGAACATGATTTTGAGGAAA+TGG - 16769:1030-1049 MS.gene066868:CDS 30.0%
!!! CACCCAATGATTTTTTTTGT+AGG + 16769:2231-2250 MS.gene066868:intergenic 30.0%
!!! TACAAGTTTTGGAAATGAAG+AGG - 16769:1664-1683 MS.gene066868:CDS 30.0%
AACGAGAAGAATTTGCGAAA+GGG - 16769:2873-2892 MS.gene066868:CDS 35.0%
AAGAAACAATTGCTTCCCTT+CGG - 16769:1412-1431 MS.gene066868:CDS 35.0%
AAGAGCTTGAATTGGAAAAG+AGG - 16769:1694-1713 MS.gene066868:CDS 35.0%
ACTACTAGAAGAACTTTCGA+GGG + 16769:2784-2803 MS.gene066868:intergenic 35.0%
ACTAGCAAGAAACAAATCAG+AGG - 16769:1743-1762 MS.gene066868:CDS 35.0%
AGAGCTTGAATTGGAAAAGA+GGG - 16769:1695-1714 MS.gene066868:CDS 35.0%
AGCAATAGCAAGGATCAAAT+TGG - 16769:3263-3282 MS.gene066868:intron 35.0%
AGCATATGAAAAGCCATCTT+TGG + 16769:2575-2594 MS.gene066868:intergenic 35.0%
CAATTCTTTGCCTGAAATCT+TGG + 16769:2661-2680 MS.gene066868:intergenic 35.0%
CTTGGAGTTGAAGAAACAAA+AGG - 16769:3151-3170 MS.gene066868:CDS 35.0%
GAATAAAGGTAGCAATAGCA+AGG - 16769:3253-3272 MS.gene066868:intron 35.0%
GACGAATTAGCGAAGTATAA+CGG - 16769:1591-1610 MS.gene066868:CDS 35.0%
GAGAAACAAAGAGCTTGAAT+TGG - 16769:1686-1705 MS.gene066868:CDS 35.0%
GATTCCTCTTCAGAGAATAA+AGG - 16769:3239-3258 MS.gene066868:intron 35.0%
TGTTATGCAAGAAGAAGTTG+AGG - 16769:3076-3095 MS.gene066868:CDS 35.0%
TTCCCAACTGAAAATGCAAT+TGG - 16769:1359-1378 MS.gene066868:CDS 35.0%
! AGACTCTGAACATGATTTTG+AGG - 16769:1024-1043 MS.gene066868:CDS 35.0%
! CTCCAATTGCATTTTCAGTT+GGG + 16769:1364-1383 MS.gene066868:intergenic 35.0%
! GATGGCTTTTCATATGCTTA+AGG - 16769:2577-2596 MS.gene066868:intron 35.0%
! TCTCCAATTGCATTTTCAGT+TGG + 16769:1365-1384 MS.gene066868:intergenic 35.0%
! TGTTTTGTGAGCAAAGTGAA+AGG - 16769:2155-2174 MS.gene066868:intron 35.0%
!!! AATTTTGGTTTCGAACCGAA+GGG + 16769:1430-1449 MS.gene066868:intergenic 35.0%
!!! TAATTTTGGTTTCGAACCGA+AGG + 16769:1431-1450 MS.gene066868:intergenic 35.0%
AAGAGCTTGTGTACCAAAGA+TGG - 16769:2559-2578 MS.gene066868:intron 40.0%
ACTGGCACATCTAATCTAGT+CGG + 16769:2064-2083 MS.gene066868:intergenic 40.0%
AGAGGGAAATAAGCCTTGAA+AGG - 16769:1292-1311 MS.gene066868:CDS 40.0%
CAACGAGAAGAATTTGCGAA+AGG - 16769:2872-2891 MS.gene066868:CDS 40.0%
CCATGAACCAACATAGTTTC+TGG + 16769:2203-2222 MS.gene066868:intergenic 40.0%
GAATTGGAAAAGAGGGAAGT+AGG - 16769:1702-1721 MS.gene066868:CDS 40.0%
GACACTCTCTTCAATCTTGT+CGG + 16769:3023-3042 MS.gene066868:intergenic 40.0%
GACTACTAGAAGAACTTTCG+AGG + 16769:2785-2804 MS.gene066868:intergenic 40.0%
GAGTTTATAAAGAGTGGCAC+TGG + 16769:2082-2101 MS.gene066868:intergenic 40.0%
TCAACATCTGATCGTTGACA+TGG + 16769:1554-1573 MS.gene066868:intergenic 40.0%
TGATAGGATTAAAAGCCTGC+TGG - 16769:3295-3314 MS.gene066868:CDS 40.0%
TGTCTCTTCGAATGCTACAT+TGG - 16769:2731-2750 MS.gene066868:CDS 40.0%
! ACGTAGGTTTTCAATGTCGA+TGG - 16769:2926-2945 MS.gene066868:CDS 40.0%
! GCTACCTTTATTCTCTGAAG+AGG + 16769:3246-3265 MS.gene066868:intergenic 40.0%
!! CCAGAAACTATGTTGGTTCA+TGG - 16769:2200-2219 MS.gene066868:intron 40.0%
!! GAGAGTGTCTTTCAGTGATT+CGG - 16769:3034-3053 MS.gene066868:CDS 40.0%
!! TCACTTCTTTTGCTTGCTGA+AGG + 16769:998-1017 MS.gene066868:intergenic 40.0%
AAGCCAAGGAAAAGTTGTGC+TGG - 16769:2966-2985 MS.gene066868:CDS 45.0%
AATCTATTGGGTTTAGCGCG+TGG - 16769:1946-1965 MS.gene066868:intron 45.0%
ACTAGAAGAACTTTCGAGGG+TGG + 16769:2781-2800 MS.gene066868:intergenic 45.0%
ACTCTCTTCAATCTTGTCGG+TGG + 16769:3020-3039 MS.gene066868:intergenic 45.0%
CAAGACTGCACCAAGATTTC+AGG - 16769:2648-2667 MS.gene066868:CDS 45.0%
GAAAACCTACGTATCAGTCC+AGG + 16769:2918-2937 MS.gene066868:intergenic 45.0%
GAGAAGAATTTGCGAAAGGG+TGG - 16769:2876-2895 MS.gene066868:CDS 45.0%
GATTTCTTGGATGACCCGAA+TGG - 16769:1971-1990 MS.gene066868:intron 45.0%
GCAGAGGAACAGATTTGACA+TGG - 16769:2533-2552 MS.gene066868:intron 45.0%
! TTAGCGCGTGGTTGATTTCT+TGG - 16769:1958-1977 MS.gene066868:intron 45.0%
!! GTATAACGGTGCTACTGCTA+TGG - 16769:1605-1624 MS.gene066868:CDS 45.0%
!!! TATTTTTTCTTTAATAATTT+TGG + 16769:1445-1464 MS.gene066868:intergenic 5.0%
CCAAGGAAAAGTTGTGCTGG+TGG - 16769:2969-2988 MS.gene066868:CDS 50.0%
GGAAATAGACCAAGCAGACC+TGG - 16769:2897-2916 MS.gene066868:CDS 50.0%
! CCACCAGCACAACTTTTCCT+TGG + 16769:2972-2991 MS.gene066868:intergenic 50.0%
! CTTGGTGCAGTCTTGTCTTG+TGG + 16769:2643-2662 MS.gene066868:intergenic 50.0%
ATACTAGCAGCTGCACCAGC+AGG + 16769:3313-3332 MS.gene066868:intergenic 55.0%
CGTATCAGTCCAGGTCTGCT+TGG + 16769:2909-2928 MS.gene066868:intergenic 55.0%
GCAGACCTGGACTGATACGT+AGG - 16769:2910-2929 MS.gene066868:CDS 55.0%


Chromosome Type Strat End Strand Name
16769 gene 992 3379 992 ID=MS.gene066868
16769 mRNA 992 3379 992 ID=MS.gene066868.t1;Parent=MS.gene066868
16769 exon 3284 3379 3284 ID=MS.gene066868.t1.exon1;Parent=MS.gene066868.t1
16769 CDS 3284 3379 3284 ID=cds.MS.gene066868.t1;Parent=MS.gene066868.t1
16769 exon 2607 3191 2607 ID=MS.gene066868.t1.exon2;Parent=MS.gene066868.t1
16769 CDS 2607 3191 2607 ID=cds.MS.gene066868.t1;Parent=MS.gene066868.t1
16769 exon 992 1921 992 ID=MS.gene066868.t1.exon3;Parent=MS.gene066868.t1
16769 CDS 992 1921 992 ID=cds.MS.gene066868.t1;Parent=MS.gene066868.t1
Gene Sequence

>MS.gene066868

ATGCCTTCAGCAAGCAAAAGAAGTGATAATTCAGACTCTGAACATGATTTTGAGGAAATGGAAAATGGCATTGCTTATGCTAGTGATGTAATACAGGTACCTTTTATGTGTTATTTTTTGTTTTTTTTGTGTGATAAGTTCAGTAGAAATGTATCCCAAAAAATTGATTTGTCTTTAATTTGCTACAGAATGAAGAAAAGAAGAAGATTAAAATTGAATTGCCACAAATTGAAGGTTGTGAAAATATGGATTATATCACTGAAAAGGAGTTGCTGCAGAATTTAATACAAAATTACAAGCAGAGGGAAATAAGCCTTGAAAGGAAACTTATTGAATTGAATAGACTGAAGGAAGAGAAATCAGTCATTTCCCAACTGAAAATGCAATTGGAGAAAAAGAGAGCAAAATTAAAATCTCTTGAAGAAACAATTGCTTCCCTTCGGTTCGAAACCAAAATTATTAAAGAAAAAATAGAAGAGGATATCATGTGTAAAATGCAGTTAGAGATTGCAAATAAAATTATAAATGAGATGGAAAGTAAGAAGGATATGAATGGAAGCCATGTCAACGATCAGATGTTGACGCTTCAACAACGAGTTGACGAATTAGCGAAGTATAACGGTGCTACTGCTATGGTGAATAAGAAACATGAACATGTTGAAGATAGAGCATTACAAGTTTTGGAAATGAAGAGGAGAAACAAAGAGCTTGAATTGGAAAAGAGGGAAGTAGGAACTAAGTTAGCTATTGCACTAGCAAGAAACAAATCAGAGGTAAGAACACTGCATCATAGAAGAAATTGATTTTTTTAGTTCATTATTCTTATGTTCATTAATATGGGACTTGGACTATTCTGAATATACCATCTTGTATTATTAAAATTATGTTTAAACCTAGTCTGCTTTTAATATGGTACTTGGATTTTGTTCGATATACCCTTTCACATTCAACACTAATCTATTGGGTTTAGCGCGTGGTTGATTTCTTGGATGACCCGAATGGATAAACTCTTATATCAAACTAGATTTTTTATATTATATTGTATATAATTCTAACTCTTTGTTACAAACCGACTAGATTAGATGTGCCAGTGCCACTCTTTATAAACTCTTTTCTGACATTATCGATTTTCGATGTGAGATTTGAATTTTTTCCAATATGATTGTTTTGTGAGCAAAGTGAAAGGAGTTTATTATAATACAATACAACCAGAAACTATGTTGGTTCATGGTTAATCCTACAAAAAAAATCATTGGGTGAAAGAACTGTAACTGTTCTCTACCTATAATTAATCAACATTACTTAAGTTATGGATACTTCAAACTAAATTTTGTTAAAATAATAGGTAGTAGGTAATAGTTATTTTGTTCTTTGTTGTCCTCTACCTTAAATTTATTGTTTAAGGCTAGTTTAAGCACTAAGCAATAAGTTTCTTATGCTTGATAATTTTTTCTACAGGAAACAAAAGTGATCCAGATACAAGAAGAAATAAGTGATTTACAACATGTTCATGAACAACTTTCAGAACAAGTTGAAATATTGCAGAGGAACAGATTTGACATGGTACAAGAGCTTGTGTACCAAAGATGGCTTTTCATATGCTTAAGGTATGAAGTTAACAATCATAATAAAAAGCATAATAGAAAGGCCACAAGACAAGACTGCACCAAGATTTCAGGCAAAGAATTGTTGTATGATGAGAAAACTCATTCATTCACATCTGATTCTGCACATGACACTGTCTCTTCGAATGCTACATTGGATGATTTTGATGAAATCGAAACTACCACCCTCGAAAGTTCTTCTAGTAGTCAAAGTAACAACAGTAGCAATTCCAGTTTGCTTAATAAATTGAAAAGATGGAAGAAATCAAAAGATTACAACGAGAAGAATTTGCGAAAGGGTGGAAATAGACCAAGCAGACCTGGACTGATACGTAGGTTTTCAATGTCGATGGTTGAATCAAATCTATCAAAGCCAAGGAAAAGTTGTGCTGGTGGTGATCCATTTATGAATCTAGAGAAACCACCGACAAGATTGAAGAGAGTGTCTTTCAGTGATTCGGTTAAAAAATTGACTTATCATGTTATGCAAGAAGAAGTTGAGGATGTAACTGATGAAAATGAAACTAACTCTCAGCTGAATTCAACTATTACATGCTTGGAGTTGAAGAAACAAAAGGAAGAAGACCAATATGATGAAATGAGCAAATCATATGAAAATGTGGTTTGCAACTATGAAAAAGTTGATTCCTCTTCAGAGAATAAAGGTAGCAATAGCAAGGATCAAATTGGTTATTCAGATGATAGGATTAAAAGCCTGCTGGTGCAGCTGCTAGTATATTTTTTCCTTTTGTTAGTCCTTTTAGCATATTTCAGGATCAAATAA

Protein sequence

>MS.gene066868.t1

MPSASKRSDNSDSEHDFEEMENGIAYASDVIQNEEKKKIKIELPQIEGCENMDYITEKELLQNLIQNYKQREISLERKLIELNRLKEEKSVISQLKMQLEKKRAKLKSLEETIASLRFETKIIKEKIEEDIMCKMQLEIANKIINEMESKKDMNGSHVNDQMLTLQQRVDELAKYNGATAMVNKKHEHVEDRALQVLEMKRRNKELELEKREVGTKLAIALARNKSEETKVIQIQEEISDLQHVHEQLSEQVEILQRNRFDMVQELVYQRWLFICLRYEVNNHNKKHNRKATRQDCTKISGKELLYDEKTHSFTSDSAHDTVSSNATLDDFDEIETTTLESSSSSQSNNSSNSSLLNKLKRWKKSKDYNEKNLRKGGNRPSRPGLIRRFSMSMVESNLSKPRKSCAGGDPFMNLEKPPTRLKRVSFSDSVKKLTYHVMQEEVEDVTDENETNSQLNSTITCLELKKQKEEDQYDEMSKSYENVVCNYEKVDSSSENKGSNSKDQIGYSDDRIKSLLVQLLVYFFLLLVLLAYFRIK