Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069095.t1 | XP_013453681.1 | 97.2 | 109 | 3 | 0 | 1 | 109 | 12 | 120 | 6.50E-42 | 179.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069095.t1 | A0A072UDK7 | 97.2 | 109 | 3 | 0 | 1 | 109 | 12 | 120 | 4.7e-42 | 179.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene069095 | -0.801754 | 7.75E-49 | -1.69E-46 |
| MS.gene049549 | MS.gene069095 | 0.812079 | 5.08E-51 | -1.69E-46 |
| MS.gene049611 | MS.gene069095 | 0.804412 | 2.19E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene069095 | -0.804838 | 1.78E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene069095 | -0.817414 | 3.34E-52 | -1.69E-46 |
| MS.gene050577 | MS.gene069095 | -0.82363 | 1.25E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene069095 | -0.80588 | 1.08E-49 | -1.69E-46 |
| MS.gene050883 | MS.gene069095 | 0.804255 | 2.36E-49 | -1.69E-46 |
| MS.gene051127 | MS.gene069095 | 0.820202 | 7.77E-53 | -1.69E-46 |
| MS.gene051138 | MS.gene069095 | 0.802484 | 5.49E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene069095 | 0.809792 | 1.59E-50 | -1.69E-46 |
| MS.gene051632 | MS.gene069095 | 0.815567 | 8.65E-52 | -1.69E-46 |
| MS.gene051973 | MS.gene069095 | 0.80861 | 2.85E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene069095 | 0.827846 | 1.25E-54 | -1.69E-46 |
| MS.gene052212 | MS.gene069095 | 0.801731 | 7.84E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene069095 | -0.809437 | 1.89E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene069095 | -0.819845 | 9.38E-53 | -1.69E-46 |
| MS.gene052606 | MS.gene069095 | 0.848913 | 4.53E-60 | -1.69E-46 |
| MS.gene053203 | MS.gene069095 | -0.807378 | 5.20E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene069095 | 0.827931 | 1.19E-54 | -1.69E-46 |
| MS.gene053421 | MS.gene069095 | 0.83667 | 8.17E-57 | -1.69E-46 |
| MS.gene05470 | MS.gene069095 | -0.825476 | 4.59E-54 | -1.69E-46 |
| MS.gene055139 | MS.gene069095 | 0.80045 | 1.43E-48 | -1.69E-46 |
| MS.gene055154 | MS.gene069095 | 0.821216 | 4.54E-53 | -1.69E-46 |
| MS.gene055158 | MS.gene069095 | 0.85448 | 1.20E-61 | -1.69E-46 |
| MS.gene055362 | MS.gene069095 | -0.80521 | 1.49E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene069095 | -0.805526 | 1.28E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene069095 | -0.812605 | 3.90E-51 | -1.69E-46 |
| MS.gene056218 | MS.gene069095 | 0.808624 | 2.83E-50 | -1.69E-46 |
| MS.gene056298 | MS.gene069095 | 0.825713 | 4.03E-54 | -1.69E-46 |
| MS.gene056504 | MS.gene069095 | -0.823828 | 1.12E-53 | -1.69E-46 |
| MS.gene056590 | MS.gene069095 | 0.800432 | 1.45E-48 | -1.69E-46 |
| MS.gene056692 | MS.gene069095 | -0.815206 | 1.04E-51 | -1.69E-46 |
| MS.gene056748 | MS.gene069095 | 0.826293 | 2.94E-54 | -1.69E-46 |
| MS.gene056856 | MS.gene069095 | 0.805194 | 1.50E-49 | -1.69E-46 |
| MS.gene056988 | MS.gene069095 | 0.81587 | 7.41E-52 | -1.69E-46 |
| MS.gene057261 | MS.gene069095 | 0.806052 | 9.92E-50 | -1.69E-46 |
| MS.gene057550 | MS.gene069095 | 0.804799 | 1.82E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene069095 | 0.850296 | 1.86E-60 | -1.69E-46 |
| MS.gene058099 | MS.gene069095 | 0.806565 | 7.73E-50 | -1.69E-46 |
| MS.gene058109 | MS.gene069095 | 0.806224 | 9.13E-50 | -1.69E-46 |
| MS.gene058111 | MS.gene069095 | 0.850962 | 1.21E-60 | -1.69E-46 |
| MS.gene058157 | MS.gene069095 | -0.817479 | 3.23E-52 | -1.69E-46 |
| MS.gene058171 | MS.gene069095 | 0.818474 | 1.92E-52 | -1.69E-46 |
| MS.gene058173 | MS.gene069095 | 0.816251 | 6.09E-52 | -1.69E-46 |
| MS.gene058175 | MS.gene069095 | 0.821054 | 4.95E-53 | -1.69E-46 |
| MS.gene058544 | MS.gene069095 | -0.800369 | 1.49E-48 | -1.69E-46 |
| MS.gene058603 | MS.gene069095 | 0.805964 | 1.04E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene069095 | -0.802208 | 6.26E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene069095 | -0.800425 | 1.45E-48 | -1.69E-46 |
| MS.gene059955 | MS.gene069095 | 0.819069 | 1.41E-52 | -1.69E-46 |
| MS.gene06000 | MS.gene069095 | -0.813266 | 2.79E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene069095 | -0.807031 | 6.16E-50 | -1.69E-46 |
| MS.gene060778 | MS.gene069095 | -0.800531 | 1.38E-48 | -1.69E-46 |
| MS.gene06077 | MS.gene069095 | -0.852447 | 4.59E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGCCTTCAGTCTTGAAAA+TGG | 0.356723 | 5.3:+19163763 | None:intergenic |
| TTATGCAGTGATGGTGGAGC+TGG | 0.397441 | 5.3:-19163860 | MS.gene069095:intron |
| TCACTGCTACTCTCAATAAC+TGG | 0.438274 | 5.3:+19163833 | None:intergenic |
| CCCTTGCCAGAGTTTACACC+AGG | 0.459349 | 5.3:+19163743 | None:intergenic |
| GGCAATGTGTTGTGCAAGAC+TGG | 0.486701 | 5.3:-19164495 | MS.gene069095:CDS |
| AGGACTGAAGGGCCTGATGC+TGG | 0.490624 | 5.3:-19164447 | MS.gene069095:CDS |
| ACAACACATTGCCCAAGCCC+TGG | 0.525921 | 5.3:+19164505 | None:intergenic |
| CCCTGGTGTAAACTCTGGCA+AGG | 0.541663 | 5.3:-19163744 | MS.gene069095:CDS |
| TATGCAGTGATGGTGGAGCT+GGG | 0.557903 | 5.3:-19163859 | MS.gene069095:intron |
| ACATTCTGCAAGTGTGGTGA+AGG | 0.561546 | 5.3:-19163797 | MS.gene069095:CDS |
| ATTGAGAGTAGCAGTGACAA+TGG | 0.563224 | 5.3:-19163827 | MS.gene069095:CDS |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5.3:-19164467 | MS.gene069095:CDS |
| GTGTTATAACTAGGACTGAA+GGG | 0.595455 | 5.3:-19164458 | MS.gene069095:CDS |
| AAACTCTTATGCAGTGATGG+TGG | 0.602169 | 5.3:-19163866 | MS.gene069095:intron |
| GAAGGCCCTGGTGTAAACTC+TGG | 0.603912 | 5.3:-19163749 | MS.gene069095:CDS |
| TGTGTTATAACTAGGACTGA+AGG | 0.610701 | 5.3:-19164459 | MS.gene069095:CDS |
| CCTGGTGTAAACTCTGGCAA+GGG | 0.620143 | 5.3:-19163743 | MS.gene069095:CDS |
| GTGTTGTGCAAGACTGGTAA+AGG | 0.626364 | 5.3:-19164489 | MS.gene069095:CDS |
| TCTGCAAGTGTGGTGAAGGG+TGG | 0.652623 | 5.3:-19163793 | MS.gene069095:CDS |
| TGTGCAAGACTGGTAAAGGT+TGG | 0.662647 | 5.3:-19164485 | MS.gene069095:CDS |
| AGTGAAACATTCTGCAAGTG+TGG | 0.671634 | 5.3:-19163803 | MS.gene069095:CDS |
| CATTCTGCAAGTGTGGTGAA+GGG | 0.681189 | 5.3:-19163796 | MS.gene069095:CDS |
| CCTTGCCAGAGTTTACACCA+GGG | 0.773669 | 5.3:+19163744 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTATTTGTTTGTAATA+TGG | - | chr5.3:19164065-19164084 | MS.gene069095:intron | 10.0% |
| !! | TAATATGAAATTGAGATTTA+AGG | + | chr5.3:19164190-19164209 | None:intergenic | 15.0% |
| !! | AATTTATCGTTCAACTTATT+AGG | + | chr5.3:19164150-19164169 | None:intergenic | 20.0% |
| !! | GCAGTTAAAATAATAATAAG+AGG | + | chr5.3:19163870-19163889 | None:intergenic | 20.0% |
| !! | TAAAATAATAATAAGAGGAG+TGG | + | chr5.3:19163865-19163884 | None:intergenic | 20.0% |
| !! | TTATTAGGTAAAAGTTCAAT+AGG | + | chr5.3:19164135-19164154 | None:intergenic | 20.0% |
| ! | AAAGTGTGAAAGACTATATT+AGG | - | chr5.3:19163996-19164015 | MS.gene069095:intron | 25.0% |
| CTAAAATGTCAAATACTGCT+TGG | + | chr5.3:19163941-19163960 | None:intergenic | 30.0% | |
| !! | GCAATCATTTTAACAATGGT+TGG | + | chr5.3:19164092-19164111 | None:intergenic | 30.0% |
| !! | TGGTGCAATCATTTTAACAA+TGG | + | chr5.3:19164096-19164115 | None:intergenic | 30.0% |
| ATCAAACTCTTATGCAGTGA+TGG | - | chr5.3:19164363-19164382 | MS.gene069095:intron | 35.0% | |
| GTGTTATAACTAGGACTGAA+GGG | - | chr5.3:19163774-19163793 | MS.gene069095:CDS | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | chr5.3:19163765-19163784 | MS.gene069095:CDS | 35.0% | |
| TAGGTAAGATCTTCATGTCA+TGG | + | chr5.3:19164116-19164135 | None:intergenic | 35.0% | |
| TGTGTTATAACTAGGACTGA+AGG | - | chr5.3:19163773-19163792 | MS.gene069095:CDS | 35.0% | |
| ! | CTGAGTTTTTATCTTGAGCT+GGG | + | chr5.3:19163706-19163725 | None:intergenic | 35.0% |
| ! | GGCAAAGTTTTTGCTAAATG+TGG | - | chr5.3:19163806-19163825 | MS.gene069095:CDS | 35.0% |
| !! | TGTTCCATTTTCAAGACTGA+AGG | - | chr5.3:19164465-19164484 | MS.gene069095:CDS | 35.0% |
| AAACTCTTATGCAGTGATGG+TGG | - | chr5.3:19164366-19164385 | MS.gene069095:intron | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | - | chr5.3:19164429-19164448 | MS.gene069095:CDS | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | - | chr5.3:19164405-19164424 | MS.gene069095:CDS | 40.0% | |
| CAAGATAAAAACTCAGGCCA+GGG | - | chr5.3:19163710-19163729 | MS.gene069095:CDS | 40.0% | |
| CCAGCTCAAGATAAAAACTC+AGG | - | chr5.3:19163704-19163723 | MS.gene069095:CDS | 40.0% | |
| TCAAGATAAAAACTCAGGCC+AGG | - | chr5.3:19163709-19163728 | MS.gene069095:CDS | 40.0% | |
| TCACTGCTACTCTCAATAAC+TGG | + | chr5.3:19164402-19164421 | None:intergenic | 40.0% | |
| ! | CCTGAGTTTTTATCTTGAGC+TGG | + | chr5.3:19163707-19163726 | None:intergenic | 40.0% |
| ACATTCTGCAAGTGTGGTGA+AGG | - | chr5.3:19164435-19164454 | MS.gene069095:CDS | 45.0% | |
| AGGGCCTTCAGTCTTGAAAA+TGG | + | chr5.3:19164472-19164491 | None:intergenic | 45.0% | |
| CATTCTGCAAGTGTGGTGAA+GGG | - | chr5.3:19164436-19164455 | MS.gene069095:CDS | 45.0% | |
| GCAAAAACTTTGCCAGCATC+AGG | + | chr5.3:19163800-19163819 | None:intergenic | 45.0% | |
| GTGTTGTGCAAGACTGGTAA+AGG | - | chr5.3:19163743-19163762 | MS.gene069095:CDS | 45.0% | |
| TGTGCAAGACTGGTAAAGGT+TGG | - | chr5.3:19163747-19163766 | MS.gene069095:CDS | 45.0% | |
| !! | ATTTTCAAGACTGAAGGCCC+TGG | - | chr5.3:19164471-19164490 | MS.gene069095:CDS | 45.0% |
| AAAAACTCAGGCCAGGGCTT+GGG | - | chr5.3:19163716-19163735 | MS.gene069095:CDS | 50.0% | |
| CCTGGTGTAAACTCTGGCAA+GGG | - | chr5.3:19164489-19164508 | MS.gene069095:CDS | 50.0% | |
| CCTTGCCAGAGTTTACACCA+GGG | + | chr5.3:19164491-19164510 | None:intergenic | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+TGG | - | chr5.3:19163737-19163756 | MS.gene069095:CDS | 50.0% | |
| TAAAAACTCAGGCCAGGGCT+TGG | - | chr5.3:19163715-19163734 | MS.gene069095:CDS | 50.0% | |
| !! | TATGCAGTGATGGTGGAGCT+GGG | - | chr5.3:19164373-19164392 | MS.gene069095:intron | 50.0% |
| !! | TTATGCAGTGATGGTGGAGC+TGG | - | chr5.3:19164372-19164391 | MS.gene069095:intron | 50.0% |
| ACAACACATTGCCCAAGCCC+TGG | + | chr5.3:19163730-19163749 | None:intergenic | 55.0% | |
| CCCTGGTGTAAACTCTGGCA+AGG | - | chr5.3:19164488-19164507 | MS.gene069095:CDS | 55.0% | |
| CCCTTGCCAGAGTTTACACC+AGG | + | chr5.3:19164492-19164511 | None:intergenic | 55.0% | |
| GAAGGCCCTGGTGTAAACTC+TGG | - | chr5.3:19164483-19164502 | MS.gene069095:CDS | 55.0% | |
| TCTGCAAGTGTGGTGAAGGG+TGG | - | chr5.3:19164439-19164458 | MS.gene069095:CDS | 55.0% | |
| AGGACTGAAGGGCCTGATGC+TGG | - | chr5.3:19163785-19163804 | MS.gene069095:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 19163697 | 19164557 | 19163697 | ID=MS.gene069095 |
| chr5.3 | mRNA | 19163697 | 19164557 | 19163697 | ID=MS.gene069095.t1;Parent=MS.gene069095 |
| chr5.3 | exon | 19164405 | 19164557 | 19164405 | ID=MS.gene069095.t1.exon1;Parent=MS.gene069095.t1 |
| chr5.3 | CDS | 19164405 | 19164557 | 19164405 | ID=cds.MS.gene069095.t1;Parent=MS.gene069095.t1 |
| chr5.3 | exon | 19163697 | 19163874 | 19163697 | ID=MS.gene069095.t1.exon2;Parent=MS.gene069095.t1 |
| chr5.3 | CDS | 19163697 | 19163874 | 19163697 | ID=cds.MS.gene069095.t1;Parent=MS.gene069095.t1 |
| Gene Sequence |
| Protein sequence |
