AlfalfaGEDB | MS.gene073003

M. sativa cultivar XinJiangDaYe / MS.gene073003

KEGG: K12859
GO: GO:0000375 GO:0000377 GO:0000398 GO:0005575 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0016070 GO:0016071 GO:0030532 GO:0032991 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0046540 GO:0071704 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:1901360 GO:1990904
PFAM: PF02966.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073003.t1	GAU28190.1	69	142	16	2	1	114	1	142	5.90E-46	193.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073003.t1	Q9FE62	66.9	142	19	2	1	114	1	142	1.6e-47	189.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073003.t1	A0A2Z6MWW0	69.0	142	16	2	1	114	1	142	4.3e-46	193.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049424	MS.gene073003	0.839577	1.46E-57	-1.69E-46
MS.gene049653	MS.gene073003	0.826785	2.24E-54	-1.69E-46
MS.gene050237	MS.gene073003	0.805159	1.53E-49	-1.69E-46
MS.gene050277	MS.gene073003	0.835484	1.63E-56	-1.69E-46
MS.gene050719	MS.gene073003	0.818467	1.93E-52	-1.69E-46
MS.gene050721	MS.gene073003	0.821878	3.20E-53	-1.69E-46
MS.gene050724	MS.gene073003	0.815749	7.88E-52	-1.69E-46
MS.gene050815	MS.gene073003	0.824899	6.29E-54	-1.69E-46
MS.gene050987	MS.gene073003	0.818321	2.08E-52	-1.69E-46
MS.gene051241	MS.gene073003	0.812825	3.49E-51	-1.69E-46
MS.gene051243	MS.gene073003	0.842821	2.05E-58	-1.69E-46
MS.gene051245	MS.gene073003	0.837789	4.23E-57	-1.69E-46
MS.gene051328	MS.gene073003	0.800987	1.11E-48	-1.69E-46
MS.gene051349	MS.gene073003	0.818302	2.10E-52	-1.69E-46
MS.gene051452	MS.gene073003	0.803163	3.97E-49	-1.69E-46
MS.gene051577	MS.gene073003	0.832759	7.87E-56	-1.69E-46
MS.gene051633	MS.gene073003	0.807468	4.98E-50	-1.69E-46
MS.gene051757	MS.gene073003	0.834377	3.10E-56	-1.69E-46
MS.gene051903	MS.gene073003	0.804776	1.84E-49	-1.69E-46
MS.gene052176	MS.gene073003	0.852963	3.27E-61	-1.69E-46
MS.gene052286	MS.gene073003	0.806414	8.32E-50	-1.69E-46
MS.gene052302	MS.gene073003	0.842229	2.94E-58	-1.69E-46
MS.gene052376	MS.gene073003	0.833453	5.29E-56	-1.69E-46
MS.gene052856	MS.gene073003	0.828905	6.93E-55	-1.69E-46
MS.gene052860	MS.gene073003	0.802477	5.51E-49	-1.69E-46
MS.gene053006	MS.gene073003	0.807843	4.15E-50	-1.69E-46
MS.gene053216	MS.gene073003	0.807278	5.47E-50	-1.69E-46
MS.gene054930	MS.gene073003	0.802099	6.59E-49	-1.69E-46
MS.gene055623	MS.gene073003	0.801889	7.28E-49	-1.69E-46
MS.gene056631	MS.gene073003	0.823482	1.35E-53	-1.69E-46
MS.gene056760	MS.gene073003	0.838256	3.20E-57	-1.69E-46
MS.gene057057	MS.gene073003	0.801462	8.90E-49	-1.69E-46
MS.gene057079	MS.gene073003	0.821387	4.15E-53	-1.69E-46
MS.gene057140	MS.gene073003	0.838711	2.44E-57	-1.69E-46
MS.gene057234	MS.gene073003	0.829493	4.99E-55	-1.69E-46
MS.gene057392	MS.gene073003	0.809089	2.25E-50	-1.69E-46
MS.gene05795	MS.gene073003	0.864362	1.28E-64	-1.69E-46
MS.gene058055	MS.gene073003	0.8185	1.90E-52	-1.69E-46
MS.gene058182	MS.gene073003	0.818177	2.25E-52	-1.69E-46
MS.gene058238	MS.gene073003	0.845854	3.13E-59	-1.69E-46
MS.gene058906	MS.gene073003	0.865406	6.04E-65	-1.69E-46
MS.gene059898	MS.gene073003	0.807536	4.82E-50	-1.69E-46
MS.gene06001	MS.gene073003	0.800741	1.25E-48	-1.69E-46
MS.gene060297	MS.gene073003	0.816366	5.74E-52	-1.69E-46
MS.gene060322	MS.gene073003	0.817818	2.71E-52	-1.69E-46
MS.gene060619	MS.gene073003	0.802304	5.98E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073003.t1	MTR_3g108770	69.014	142	16	2	1	114	1	142	3.88e-64	191
MS.gene073003.t1	MTR_1g045900	68.310	142	17	2	1	114	1	142	1.30e-63	190
MS.gene073003.t1	MTR_4g075190	62.937	143	23	3	1	114	1	142	1.41e-59	179
MS.gene073003.t1	MTR_5g075850	72.222	54	13	1	63	114	1	54	4.28e-22	82.4
MS.gene073003.t1	MTR_5g075820	68.889	45	14	0	70	114	110	154	1.13e-16	71.2
MS.gene073003.t1	MTR_1g054195	71.111	45	12	1	70	113	175	219	1.43e-16	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073003.t1	AT5G08290	66.901	142	19	2	1	114	1	142	1.08e-62	187
MS.gene073003.t1	AT5G08290	98.214	56	1	0	59	114	47	102	3.21e-36	119

Find 34 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTCTCGTTGTCATCCGTTT+TGG	0.320244	3.4:-94162006	MS.gene073003:CDS
GTACTTGGTGGAGTAATCTT+TGG	0.329915	3.4:+94160989	None:intergenic
GAAACAACAACAAGATCAAT+TGG	0.342414	3.4:-94161097	MS.gene073003:CDS
TTGAGAAGCAGAAACTATAT+TGG	0.344368	3.4:+94161625	None:intergenic
GCCTCACTTGCATTCGGGAT+GGG	0.362710	3.4:-94162061	MS.gene073003:CDS
TGAGAAGCAGAAACTATATT+GGG	0.366237	3.4:+94161626	None:intergenic
CATGCTGCCTCACTTGCATT+CGG	0.374586	3.4:-94162067	MS.gene073003:CDS
TTCGGGATGGGCTGTTGATC+AGG	0.384531	3.4:-94162049	MS.gene073003:CDS
TGATGTATTTACAGAGAAAC+AGG	0.387808	3.4:+94161590	None:intergenic
TACTTGGTGGAGTAATCTTT+GGG	0.403435	3.4:+94160990	None:intergenic
TTGGGCTATGAAGGACAAAC+AGG	0.451623	3.4:-94161078	MS.gene073003:CDS
CAAGATCAATTGGGCTATGA+AGG	0.451707	3.4:-94161087	MS.gene073003:CDS
TACATTCTAGTAACGGTACT+TGG	0.457670	3.4:+94160974	None:intergenic
TGCCTCACTTGCATTCGGGA+TGG	0.468994	3.4:-94162062	MS.gene073003:CDS
TGTTGTTGTTTCCAGTTCCA+AGG	0.469561	3.4:+94161108	None:intergenic
ATCATGATAGACCTTGGAAC+TGG	0.486256	3.4:-94161119	MS.gene073003:CDS
ATGCTGCCTCACTTGCATTC+GGG	0.490375	3.4:-94162066	MS.gene073003:CDS
AAACAACAACAAGATCAATT+GGG	0.514098	3.4:-94161096	MS.gene073003:CDS
TCTACAGAGGTGCAAGGAAG+GGG	0.542753	3.4:-94161031	MS.gene073003:CDS
CATCCCAATCGTGACCAAAA+CGG	0.547656	3.4:+94161992	None:intergenic
TGTCTACAGAGGTGCAAGGA+AGG	0.552161	3.4:-94161033	MS.gene073003:CDS
GTCTACAGAGGTGCAAGGAA+GGG	0.554185	3.4:-94161032	MS.gene073003:CDS
AAGCACATCATGATAGACCT+TGG	0.567283	3.4:-94161125	MS.gene073003:CDS
ATTCTAGTAACGGTACTTGG+TGG	0.586412	3.4:+94160977	None:intergenic
TTGGGATGAGACTTGTATGC+AGG	0.595858	3.4:-94161977	MS.gene073003:intron
TCAGGCAATCCTCGCTGAGG+AGG	0.599877	3.4:-94162031	MS.gene073003:CDS
AGAGGTGCAAGGAAGGGGCG+TGG	0.611606	3.4:-94161026	MS.gene073003:CDS
GCCCATCCCGAATGCAAGTG+AGG	0.615016	3.4:+94162060	None:intergenic
GAGAAGCAGAAACTATATTG+GGG	0.656478	3.4:+94161627	None:intergenic
AGATTGATACATTCTAGTAA+CGG	0.666479	3.4:+94160967	None:intergenic
TGATCAGGCAATCCTCGCTG+AGG	0.692917	3.4:-94162034	MS.gene073003:CDS
AGACTGTCTACAGAGGTGCA+AGG	0.715363	3.4:-94161037	MS.gene073003:CDS
ATCATTGAGACTGTCTACAG+AGG	0.717834	3.4:-94161044	MS.gene073003:CDS
CGAGACGTTCCTCCTCAGCG+AGG	0.755149	3.4:+94162022	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGCTTTTTTTTTTTTTTTT+TGG	-	chr3.4:94161755-94161774	MS.gene073003:intron	10.0%
!!	TTATGTCCTATATATATTAT+TGG	+	chr3.4:94161304-94161323	None:intergenic	15.0%
!!	AAACAGCCAATAATATATAT+AGG	-	chr3.4:94161295-94161314	MS.gene073003:intron	20.0%
!!	AGAGATAAAGTAATGAATTT+AGG	-	chr3.4:94161325-94161344	MS.gene073003:intron	20.0%
!!	ATCAACCTAAAATTAAGAAT+TGG	+	chr3.4:94161683-94161702	None:intergenic	20.0%
!!	GAGATAAAGTAATGAATTTA+GGG	-	chr3.4:94161326-94161345	MS.gene073003:intron	20.0%
!!!	TCTTAATTTTAGGTTGATAA+GGG	-	chr3.4:94161685-94161704	MS.gene073003:intron	20.0%
!!!	TTCTTAATTTTAGGTTGATA+AGG	-	chr3.4:94161684-94161703	MS.gene073003:intron	20.0%
!	AAACAACAACAAGATCAATT+GGG	-	chr3.4:94161959-94161978	MS.gene073003:intron	25.0%
!	ATAAAGTAATGAATTTAGGG+AGG	-	chr3.4:94161329-94161348	MS.gene073003:intron	25.0%
!	ATATCAAGAACTGCTTAATT+TGG	-	chr3.4:94161170-94161189	MS.gene073003:intron	25.0%
!	GCATGAAACAAATTTGAATT+TGG	+	chr3.4:94161374-94161393	None:intergenic	25.0%
!!	ATGCACCAATTCTTAATTTT+AGG	-	chr3.4:94161675-94161694	MS.gene073003:intron	25.0%
	AAACTATATTGGGGAAATAC+TGG	+	chr3.4:94161422-94161441	None:intergenic	30.0%
	CCATCACAATAACAATTTCA+TGG	-	chr3.4:94161575-94161594	MS.gene073003:intron	30.0%
	GAAACAACAACAAGATCAAT+TGG	-	chr3.4:94161958-94161977	MS.gene073003:intron	30.0%
	TCATACATAGTGTTAAAGTC+AGG	+	chr3.4:94161870-94161889	None:intergenic	30.0%
	TGAGAAGCAGAAACTATATT+GGG	+	chr3.4:94161432-94161451	None:intergenic	30.0%
	TGAGCGAAACTTGATTAATT+AGG	-	chr3.4:94161714-94161733	MS.gene073003:intron	30.0%
	TGATGTATTTACAGAGAAAC+AGG	+	chr3.4:94161468-94161487	None:intergenic	30.0%
	TTGAGAAGCAGAAACTATAT+TGG	+	chr3.4:94161433-94161452	None:intergenic	30.0%
!	CCATGAAATTGTTATTGTGA+TGG	+	chr3.4:94161578-94161597	None:intergenic	30.0%
	AAGAAGAACATAACTGTGGA+AGG	+	chr3.4:94161903-94161922	None:intergenic	35.0%
	AAGTAATGAATTTAGGGAGG+TGG	-	chr3.4:94161332-94161351	MS.gene073003:intron	35.0%
	AGAAGAACATAACTGTGGAA+GGG	+	chr3.4:94161902-94161921	None:intergenic	35.0%
	CCACAGTTATGTTCTTCTTT+AGG	-	chr3.4:94161904-94161923	MS.gene073003:intron	35.0%
	CCTAAAGAAGAACATAACTG+TGG	+	chr3.4:94161907-94161926	None:intergenic	35.0%
	GAGAAGCAGAAACTATATTG+GGG	+	chr3.4:94161431-94161450	None:intergenic	35.0%
	TATCATAATACCAATCGCAG+TGG	+	chr3.4:94161626-94161645	None:intergenic	35.0%
!	TACTTGGTGGAGTAATCTTT+GGG	+	chr3.4:94162068-94162087	None:intergenic	35.0%
!!!	TTTTTTGGTGTGTGTCAAGA+TGG	-	chr3.4:94161770-94161789	MS.gene073003:intron	35.0%
	AAGCACATCATGATAGACCT+TGG	-	chr3.4:94161930-94161949	MS.gene073003:intron	40.0%
	ATCATGATAGACCTTGGAAC+TGG	-	chr3.4:94161936-94161955	MS.gene073003:intron	40.0%
	ATCATTGAGACTGTCTACAG+AGG	-	chr3.4:94162011-94162030	MS.gene073003:CDS	40.0%
	CAAGATCAATTGGGCTATGA+AGG	-	chr3.4:94161968-94161987	MS.gene073003:intron	40.0%
!	CTTTGCTGTGATATACCTTG+TGG	-	chr3.4:94161830-94161849	MS.gene073003:intron	40.0%
!	GTACTTGGTGGAGTAATCTT+TGG	+	chr3.4:94162069-94162088	None:intergenic	40.0%
!!	TGTTGTTGTTTCCAGTTCCA+AGG	+	chr3.4:94161950-94161969	None:intergenic	40.0%
	ATACCTTGTGGACATCACAG+AGG	-	chr3.4:94161842-94161861	MS.gene073003:intron	45.0%
	CATCCCAATCGTGACCAAAA+CGG	+	chr3.4:94161066-94161085	None:intergenic	45.0%
	TTGGGATGAGACTTGTATGC+AGG	-	chr3.4:94161078-94161097	MS.gene073003:CDS	45.0%
	TTGGGCTATGAAGGACAAAC+AGG	-	chr3.4:94161977-94161996	MS.gene073003:intron	45.0%
!!	CATCCGTTTTGGTCACGATT+GGG	-	chr3.4:94161060-94161079	MS.gene073003:CDS	45.0%
!!	TCATCCGTTTTGGTCACGAT+TGG	-	chr3.4:94161059-94161078	MS.gene073003:CDS	45.0%
	ATGCTGCCTCACTTGCATTC+GGG	-	chr3.4:94160989-94161008	MS.gene073003:CDS	50.0%
	CATGCTGCCTCACTTGCATT+CGG	-	chr3.4:94160988-94161007	MS.gene073003:CDS	50.0%
	GGTGTGTGTCAAGATGGATG+AGG	-	chr3.4:94161776-94161795	MS.gene073003:intron	50.0%
!	AGACTGTCTACAGAGGTGCA+AGG	-	chr3.4:94162018-94162037	MS.gene073003:CDS	50.0%
!	CGTCTCGTTGTCATCCGTTT+TGG	-	chr3.4:94161049-94161068	MS.gene073003:CDS	50.0%
!	GTCTACAGAGGTGCAAGGAA+GGG	-	chr3.4:94162023-94162042	MS.gene073003:CDS	50.0%
!	TCTACAGAGGTGCAAGGAAG+GGG	-	chr3.4:94162024-94162043	MS.gene073003:CDS	50.0%
!	TGTCTACAGAGGTGCAAGGA+AGG	-	chr3.4:94162022-94162041	MS.gene073003:CDS	50.0%
!!	GATGGATGAGGTGCTGTCAT+CGG	-	chr3.4:94161788-94161807	MS.gene073003:intron	50.0%
	ACCAATCGCAGTGGTGTTCG+TGG	+	chr3.4:94161617-94161636	None:intergenic	55.0%
	ACCACGAACACCACTGCGAT+TGG	-	chr3.4:94161613-94161632	MS.gene073003:CDS	55.0%
	GCACCTCTGTGATGTCCACA+AGG	+	chr3.4:94161848-94161867	None:intergenic	55.0%
	GCCTCACTTGCATTCGGGAT+GGG	-	chr3.4:94160994-94161013	MS.gene073003:CDS	55.0%
	TGATCAGGCAATCCTCGCTG+AGG	-	chr3.4:94161021-94161040	MS.gene073003:CDS	55.0%
	TGCCTCACTTGCATTCGGGA+TGG	-	chr3.4:94160993-94161012	MS.gene073003:CDS	55.0%
!!	TTCGGGATGGGCTGTTGATC+AGG	-	chr3.4:94161006-94161025	MS.gene073003:CDS	55.0%
	GCCCATCCCGAATGCAAGTG+AGG	+	chr3.4:94160998-94161017	None:intergenic	60.0%
	TCAGGCAATCCTCGCTGAGG+AGG	-	chr3.4:94161024-94161043	MS.gene073003:CDS	60.0%
	CGAGACGTTCCTCCTCAGCG+AGG	+	chr3.4:94161036-94161055	None:intergenic	65.0%
!	AGAGGTGCAAGGAAGGGGCG+TGG	-	chr3.4:94162029-94162048	MS.gene073003:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	94160980	94162097	94160980	ID=MS.gene073003
chr3.4	mRNA	94160980	94162097	94160980	ID=MS.gene073003.t1;Parent=MS.gene073003
chr3.4	exon	94161978	94162097	94161978	ID=MS.gene073003.t1.exon1;Parent=MS.gene073003.t1
chr3.4	CDS	94161978	94162097	94161978	ID=cds.MS.gene073003.t1;Parent=MS.gene073003.t1
chr3.4	exon	94161602	94161654	94161602	ID=MS.gene073003.t1.exon2;Parent=MS.gene073003.t1
chr3.4	CDS	94161602	94161654	94161602	ID=cds.MS.gene073003.t1;Parent=MS.gene073003.t1
chr3.4	exon	94160980	94161151	94160980	ID=MS.gene073003.t1.exon3;Parent=MS.gene073003.t1
chr3.4	CDS	94160980	94161151	94160980	ID=cds.MS.gene073003.t1;Parent=MS.gene073003.t1

Gene Sequence

>MS.gene073003

ATGTCGTACATGCTGCCTCACTTGCATTCGGGATGGGCTGTTGATCAGGCAATCCTCGCTGAGGAGGAACGTCTCGTTGTCATCCGTTTTGGTCACGATTGGGATGAGACTTGTATGCAGGTACGTATAACATCAATCAATCAATCAATCAGTGTTTTATATTTTCATCTACTCATTCATTCACAGTAAAATATCAAGAACTGCTTAATTTGGAATGATGCTTTTTTATTAAGCAACAATTTCTTGTATGTATGTATGAGTTGAACAGTGCTATATAGCATGAATCTTTCTTGTATGAAAACAATACAGAACAGAAAACAGCCAATAATATATATAGGACATAAGAGAGATAAAGTAATGAATTTAGGGAGGTGGAGATTAATAAGATGCTCCAAATTCAAATTTGTTTCATGCTGATTTGTGTAGTTTTATTTCGTAACCAGTATTTCCCCAATATAGTTTCTGCTTCTCAACTTTTTCTGAAGCCTGTTTCTCTGTAAATACATCAACATCTCTACATGCTTAGAATATTTCTCTTTGCATATGAAGAATCATAAAGCTAATTCAAACTGAGTCATAGAGCTATTATCTTCAACCATCACAATAACAATTTCATGGATTTTACATGATGATACCACGAACACCACTGCGATTGGTATTATGATACATAATGTTTGTTTCTTTCAACAATCATCATGCACCAATTCTTAATTTTAGGTTGATAAGGGTTATATTGAGCGAAACTTGATTAATTAGGTTAGTGCTGCTGTTTGATTTGCTTTTTTTTTTTTTTTTTGGTGTGTGTCAAGATGGATGAGGTGCTGTCATCGGTTGCAGAGACAATAAAGAACTTTGCTGTGATATACCTTGTGGACATCACAGAGGTGCCTGACTTTAACACTATGTATGAGCTGTATGACCCTTCCACAGTTATGTTCTTCTTTAGGAACAAGCACATCATGATAGACCTTGGAACTGGAAACAACAACAAGATCAATTGGGCTATGAAGGACAAACAGGAGTTCATTGACATCATTGAGACTGTCTACAGAGGTGCAAGGAAGGGGCGTGGTCTTGTCATTGCTCCCAAAGATTACTCCACCAAGTACCGTTACTAG

Protein sequence

>MS.gene073003.t1

MSYMLPHLHSGWAVDQAILAEEERLVVIRFGHDWDETCMQYFPNIVSASQLFLKPVSLNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGARKGRGLVIAPKDYSTKYRY