Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08041.t1 | XP_003600399.2 | 98.9 | 95 | 1 | 0 | 10 | 104 | 120 | 214 | 3.50E-45 | 190.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08041.t1 | Q944B0 | 77.9 | 86 | 19 | 0 | 10 | 95 | 122 | 207 | 7.7e-33 | 141.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08041.t1 | G7IXS8 | 98.9 | 95 | 1 | 0 | 10 | 104 | 120 | 214 | 2.5e-45 | 190.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene08041 | 0.823964 | 1.04E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene08041 | 0.804692 | 1.91E-49 | -1.69E-46 |
| MS.gene049662 | MS.gene08041 | 0.818883 | 1.55E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene08041 | 0.834025 | 3.80E-56 | -1.69E-46 |
| MS.gene049910 | MS.gene08041 | 0.805507 | 1.29E-49 | -1.69E-46 |
| MS.gene050076 | MS.gene08041 | 0.830149 | 3.45E-55 | -1.69E-46 |
| MS.gene050301 | MS.gene08041 | 0.842656 | 2.26E-58 | -1.69E-46 |
| MS.gene050397 | MS.gene08041 | 0.813031 | 3.14E-51 | -1.69E-46 |
| MS.gene050404 | MS.gene08041 | 0.830144 | 3.47E-55 | -1.69E-46 |
| MS.gene050406 | MS.gene08041 | 0.806971 | 6.35E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene08041 | 0.844168 | 8.93E-59 | -1.69E-46 |
| MS.gene05101 | MS.gene08041 | 0.811065 | 8.44E-51 | -1.69E-46 |
| MS.gene051471 | MS.gene08041 | 0.839195 | 1.83E-57 | -1.69E-46 |
| MS.gene051582 | MS.gene08041 | 0.810113 | 1.35E-50 | -1.69E-46 |
| MS.gene052078 | MS.gene08041 | 0.824933 | 6.17E-54 | -1.69E-46 |
| MS.gene052178 | MS.gene08041 | 0.844734 | 6.29E-59 | -1.69E-46 |
| MS.gene052310 | MS.gene08041 | 0.826752 | 2.28E-54 | -1.69E-46 |
| MS.gene052326 | MS.gene08041 | 0.804766 | 1.85E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene08041 | 0.819299 | 1.25E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene08041 | 0.823913 | 1.07E-53 | -1.69E-46 |
| MS.gene053209 | MS.gene08041 | 0.811459 | 6.93E-51 | -1.69E-46 |
| MS.gene053557 | MS.gene08041 | 0.839919 | 1.19E-57 | -1.69E-46 |
| MS.gene053836 | MS.gene08041 | 0.823656 | 1.23E-53 | -1.69E-46 |
| MS.gene053971 | MS.gene08041 | 0.827406 | 1.59E-54 | -1.69E-46 |
| MS.gene054376 | MS.gene08041 | 0.80088 | 1.17E-48 | -1.69E-46 |
| MS.gene055116 | MS.gene08041 | 0.822149 | 2.76E-53 | -1.69E-46 |
| MS.gene055277 | MS.gene08041 | 0.842366 | 2.70E-58 | -1.69E-46 |
| MS.gene055422 | MS.gene08041 | 0.839152 | 1.88E-57 | -1.69E-46 |
| MS.gene055428 | MS.gene08041 | 0.854466 | 1.21E-61 | -1.69E-46 |
| MS.gene056451 | MS.gene08041 | 0.802856 | 4.60E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene08041 | 0.829363 | 5.37E-55 | -1.69E-46 |
| MS.gene05702 | MS.gene08041 | 0.861785 | 8.04E-64 | -1.69E-46 |
| MS.gene05733 | MS.gene08041 | 0.814874 | 1.23E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene08041 | 0.816212 | 6.21E-52 | -1.69E-46 |
| MS.gene058082 | MS.gene08041 | 0.816671 | 4.90E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene08041 | 0.818336 | 2.07E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene08041 | 0.847983 | 8.19E-60 | -1.69E-46 |
| MS.gene058276 | MS.gene08041 | 0.801558 | 8.51E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene08041 | 0.817185 | 3.76E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene08041 | 0.810272 | 1.25E-50 | -1.69E-46 |
| MS.gene058787 | MS.gene08041 | 0.805723 | 1.16E-49 | -1.69E-46 |
| MS.gene058845 | MS.gene08041 | 0.811254 | 7.67E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene08041 | 0.803458 | 3.45E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene08041 | 0.813479 | 2.51E-51 | -1.69E-46 |
| MS.gene059597 | MS.gene08041 | 0.816918 | 4.32E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene08041 | 0.818417 | 1.98E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene08041 | 0.844406 | 7.71E-59 | -1.69E-46 |
| MS.gene059695 | MS.gene08041 | 0.840441 | 8.68E-58 | -1.69E-46 |
| MS.gene05984 | MS.gene08041 | 0.803983 | 2.69E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene08041 | 0.86739 | 1.42E-65 | -1.69E-46 |
| MS.gene06037 | MS.gene08041 | 0.801887 | 7.28E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene08041 | 0.869963 | 2.09E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08041.t1 | MTR_3g060750 | 98.947 | 95 | 1 | 0 | 10 | 104 | 120 | 214 | 8.79e-64 | 192 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08041.t1 | AT4G26555 | 77.907 | 86 | 19 | 0 | 10 | 95 | 76 | 161 | 1.05e-43 | 140 |
| MS.gene08041.t1 | AT4G26555 | 77.907 | 86 | 19 | 0 | 10 | 95 | 122 | 207 | 1.12e-43 | 141 |
| MS.gene08041.t1 | AT4G26555 | 66.667 | 39 | 13 | 0 | 10 | 48 | 122 | 160 | 7.79e-11 | 56.2 |
Find 27 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATAACTGTCCTAATGTTT+TGG | 0.273176 | 3.1:+85893326 | None:intergenic |
| TTGTTTAAAACAGATGATAC+TGG | 0.286671 | 3.1:+85894069 | MS.gene08041:intron |
| TTCTCATGCTCAAGAGCCTT+TGG | 0.319830 | 3.1:+85894622 | MS.gene08041:CDS |
| GATTACTTTCTCCATTGAAT+TGG | 0.366396 | 3.1:-85893381 | None:intergenic |
| AGTGCTAGACTAACCTCTTC+AGG | 0.368074 | 3.1:-85894352 | None:intergenic |
| TCATCCAGAGGAAGTATAAC+AGG | 0.388887 | 3.1:-85893403 | None:intergenic |
| AGACTAACCTCTTCAGGTAT+TGG | 0.406636 | 3.1:-85894346 | None:intergenic |
| TAATCCTGTTATACTTCCTC+TGG | 0.433615 | 3.1:+85893399 | MS.gene08041:CDS |
| GGGTTAAAGCAGGTCTTGAC+TGG | 0.462835 | 3.1:+85894091 | MS.gene08041:CDS |
| TGTTTAAAACAGATGATACT+GGG | 0.504041 | 3.1:+85894070 | MS.gene08041:intron |
| ACTTCCTCTGGATGAGGACC+AGG | 0.515288 | 3.1:+85893411 | MS.gene08041:CDS |
| TAATTAAATTCAGATTGTTG+TGG | 0.529196 | 3.1:-85894680 | None:intergenic |
| GAGAAAGAAGGCTGCGTCGA+GGG | 0.529313 | 3.1:-85894604 | None:intergenic |
| GCTCTTGAGCATGAGAAAGA+AGG | 0.531879 | 3.1:-85894616 | None:intergenic |
| CTTGAAGCCAATACCTGAAG+AGG | 0.536259 | 3.1:+85894339 | MS.gene08041:CDS |
| GTCTTGACTGGCATGAGAGT+TGG | 0.540633 | 3.1:+85894103 | MS.gene08041:CDS |
| TTGACTGGCATGAGAGTTGG+TGG | 0.545256 | 3.1:+85894106 | MS.gene08041:CDS |
| AGTACAGTAGACCAATTCAA+TGG | 0.553972 | 3.1:+85893370 | MS.gene08041:CDS |
| TGTTATACTTCCTCTGGATG+AGG | 0.558694 | 3.1:+85893405 | MS.gene08041:CDS |
| GATGATACTGGGGTTAAAGC+AGG | 0.562802 | 3.1:+85894081 | MS.gene08041:intron |
| GCATTGATTCCACCTTCTGT+AGG | 0.568687 | 3.1:+85894301 | MS.gene08041:CDS |
| TCTACATATCCTACAGAAGG+TGG | 0.586098 | 3.1:-85894310 | None:intergenic |
| TCGTCTACATATCCTACAGA+AGG | 0.627789 | 3.1:-85894313 | None:intergenic |
| TGAGAAAGAAGGCTGCGTCG+AGG | 0.648007 | 3.1:-85894605 | None:intergenic |
| AGTTGAACTTCAAATACCAA+AGG | 0.649057 | 3.1:-85894638 | None:intergenic |
| GTTTAAAACAGATGATACTG+GGG | 0.650883 | 3.1:+85894071 | MS.gene08041:intron |
| CTAACCTGGTCCTCATCCAG+AGG | 0.693869 | 3.1:-85893415 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACGTTTAAAAAAAAATTGA+CGG | - | chr3.1:85894402-85894421 | None:intergenic | 15.0% |
| !! | GGAAAATGATATTATAAACA+CGG | + | chr3.1:85893565-85893584 | MS.gene08041:intron | 20.0% |
| !! | TAAAATGTTATGTTAGTAGT+TGG | + | chr3.1:85894434-85894453 | MS.gene08041:intron | 20.0% |
| !! | TAATTAAATTCAGATTGTTG+TGG | - | chr3.1:85894683-85894702 | None:intergenic | 20.0% |
| !! | TATAAGAAAGAAAAGAACAA+TGG | - | chr3.1:85893860-85893879 | None:intergenic | 20.0% |
| !! | TTAATTTAAACATCCAAACA+AGG | - | chr3.1:85894487-85894506 | None:intergenic | 20.0% |
| !!! | TGATTAATAGTCAAATTTTG+TGG | - | chr3.1:85893957-85893976 | None:intergenic | 20.0% |
| !!! | TGTATTGATGACTTTTTTTT+TGG | + | chr3.1:85894519-85894538 | MS.gene08041:intron | 20.0% |
| ! | ATGAAGGAATCTATCTAATA+AGG | - | chr3.1:85893751-85893770 | None:intergenic | 25.0% |
| ! | CTTATATTCTGCAAATTTGA+AGG | + | chr3.1:85893874-85893893 | MS.gene08041:intron | 25.0% |
| ! | TATTGTTTCAAATCTAGCAA+AGG | - | chr3.1:85893919-85893938 | None:intergenic | 25.0% |
| ! | TTGAAAGAAATAAACTAACC+TGG | - | chr3.1:85893432-85893451 | None:intergenic | 25.0% |
| !! | AGTTTATTTCTTTCAACTTG+TGG | + | chr3.1:85893436-85893455 | MS.gene08041:intron | 25.0% |
| !! | CTGATGATGTTTCTTATATA+TGG | + | chr3.1:85893496-85893515 | MS.gene08041:intron | 25.0% |
| !! | GTTTATTTCTTTCAACTTGT+GGG | + | chr3.1:85893437-85893456 | MS.gene08041:intron | 25.0% |
| !! | TGTTTAAAACAGATGATACT+GGG | + | chr3.1:85894070-85894089 | MS.gene08041:intron | 25.0% |
| !! | TTGTTTAAAACAGATGATAC+TGG | + | chr3.1:85894069-85894088 | MS.gene08041:intron | 25.0% |
| !! | TTTTTGTGTTTAATGCAGTT+TGG | + | chr3.1:85894581-85894600 | MS.gene08041:intron | 25.0% |
| !!! | AAATCAGTATTTTGACAATC+TGG | + | chr3.1:85894161-85894180 | MS.gene08041:intron | 25.0% |
| !!! | ACTTGAGTTGAAGAATATAA+AGG | - | chr3.1:85894001-85894020 | None:intergenic | 25.0% |
| !!! | CTTGAGTTGAAGAATATAAA+GGG | - | chr3.1:85894000-85894019 | None:intergenic | 25.0% |
| AAGTATAGCAATGTCTATGA+AGG | - | chr3.1:85893767-85893786 | None:intergenic | 30.0% | |
| ACTTGGATACAATCATACAA+AGG | + | chr3.1:85894372-85894391 | MS.gene08041:intron | 30.0% | |
| AGTTGAACTTCAAATACCAA+AGG | - | chr3.1:85894641-85894660 | None:intergenic | 30.0% | |
| GATTACTTTCTCCATTGAAT+TGG | - | chr3.1:85893384-85893403 | None:intergenic | 30.0% | |
| GCATATCGCAATTTATAAGT+GGG | + | chr3.1:85893649-85893668 | MS.gene08041:intron | 30.0% | |
| TGCATATCGCAATTTATAAG+TGG | + | chr3.1:85893648-85893667 | MS.gene08041:intron | 30.0% | |
| ! | GTTTAAAACAGATGATACTG+GGG | + | chr3.1:85894071-85894090 | MS.gene08041:intron | 30.0% |
| ! | TAGACATTGCTATACTTTTC+AGG | + | chr3.1:85893770-85893789 | MS.gene08041:intron | 30.0% |
| ! | TGTATCTTTTGATGTCATTG+TGG | + | chr3.1:85894245-85894264 | MS.gene08041:intron | 30.0% |
| !! | CAGATTTGCTATTACAAATC+TGG | + | chr3.1:85893815-85893834 | MS.gene08041:intron | 30.0% |
| !! | TGTCAAAATACTGATTTGTC+AGG | - | chr3.1:85894158-85894177 | None:intergenic | 30.0% |
| !!! | TGACTGTTTTTTTTTGTGTG+TGG | + | chr3.1:85894218-85894237 | MS.gene08041:intron | 30.0% |
| AAACACATTTCAGAGTTCTC+TGG | - | chr3.1:85893465-85893484 | None:intergenic | 35.0% | |
| AAGCATCTAATTGTAGTCAC+TGG | + | chr3.1:85893725-85893744 | MS.gene08041:intron | 35.0% | |
| AATTGCGGTTACACTACATT+GGG | + | chr3.1:85893599-85893618 | MS.gene08041:intron | 35.0% | |
| ACCAAATTATACAGGACTAG+TGG | - | chr3.1:85894196-85894215 | None:intergenic | 35.0% | |
| ACTCAAGTTACCATAAACTG+AGG | + | chr3.1:85894013-85894032 | MS.gene08041:intron | 35.0% | |
| AGTACAGTAGACCAATTCAA+TGG | + | chr3.1:85893370-85893389 | MS.gene08041:CDS | 35.0% | |
| CAGATTATCTCCTCAGTTTA+TGG | - | chr3.1:85894026-85894045 | None:intergenic | 35.0% | |
| CAGTCATGACCAAATTATAC+AGG | - | chr3.1:85894204-85894223 | None:intergenic | 35.0% | |
| TAATCCTGTTATACTTCCTC+TGG | + | chr3.1:85893399-85893418 | MS.gene08041:CDS | 35.0% | |
| TCATCAATACATAGCTGAAG+TGG | - | chr3.1:85894510-85894529 | None:intergenic | 35.0% | |
| TCCACTAGTCCTGTATAATT+TGG | + | chr3.1:85894192-85894211 | MS.gene08041:intron | 35.0% | |
| !! | ACGGTTCTAAATTGCAATTG+CGG | + | chr3.1:85893584-85893603 | MS.gene08041:intron | 35.0% |
| !! | GTGGGTTGATTATTGTAACA+TGG | + | chr3.1:85893667-85893686 | MS.gene08041:intron | 35.0% |
| ACTTTACGCTCAACCTTGTT+TGG | + | chr3.1:85894471-85894490 | MS.gene08041:intron | 40.0% | |
| AGACTAACCTCTTCAGGTAT+TGG | - | chr3.1:85894349-85894368 | None:intergenic | 40.0% | |
| ATTGCGGTTACACTACATTG+GGG | + | chr3.1:85893600-85893619 | MS.gene08041:intron | 40.0% | |
| CAATTGCGGTTACACTACAT+TGG | + | chr3.1:85893598-85893617 | MS.gene08041:intron | 40.0% | |
| TCGTCTACATATCCTACAGA+AGG | - | chr3.1:85894316-85894335 | None:intergenic | 40.0% | |
| TCTACATATCCTACAGAAGG+TGG | - | chr3.1:85894313-85894332 | None:intergenic | 40.0% | |
| TGTTATACTTCCTCTGGATG+AGG | + | chr3.1:85893405-85893424 | MS.gene08041:CDS | 40.0% | |
| ! | TCATCCAGAGGAAGTATAAC+AGG | - | chr3.1:85893406-85893425 | None:intergenic | 40.0% |
| AAGGGCGACTGAAGAATACT+AGG | - | chr3.1:85893982-85894001 | None:intergenic | 45.0% | |
| AGTGCTAGACTAACCTCTTC+AGG | - | chr3.1:85894355-85894374 | None:intergenic | 45.0% | |
| CACTACATTGGGGTTAGATG+CGG | + | chr3.1:85893610-85893629 | MS.gene08041:intron | 45.0% | |
| CTTGAAGCCAATACCTGAAG+AGG | + | chr3.1:85894339-85894358 | MS.gene08041:CDS | 45.0% | |
| GATATGCAACAACAACCGCA+AGG | - | chr3.1:85893636-85893655 | None:intergenic | 45.0% | |
| GATGATACTGGGGTTAAAGC+AGG | + | chr3.1:85894081-85894100 | MS.gene08041:intron | 45.0% | |
| GATGTCATTGTGGTCACTCT+TGG | + | chr3.1:85894255-85894274 | MS.gene08041:intron | 45.0% | |
| GCTCTTGAGCATGAGAAAGA+AGG | - | chr3.1:85894619-85894638 | None:intergenic | 45.0% | |
| TCACTCTTGGCTTCACTTGT+AGG | + | chr3.1:85894268-85894287 | MS.gene08041:intron | 45.0% | |
| TTCTCATGCTCAAGAGCCTT+TGG | + | chr3.1:85894622-85894641 | MS.gene08041:CDS | 45.0% | |
| !! | GAAGAGGTTAGTCTAGCACT+TGG | + | chr3.1:85894355-85894374 | MS.gene08041:intron | 45.0% |
| !! | GCATTGATTCCACCTTCTGT+AGG | + | chr3.1:85894301-85894320 | MS.gene08041:CDS | 45.0% |
| !!! | AAATTTTTTTATTAAGTAAT+TGG | + | chr3.1:85893544-85893563 | MS.gene08041:intron | 5.0% |
| GGGTTAAAGCAGGTCTTGAC+TGG | + | chr3.1:85894091-85894110 | MS.gene08041:CDS | 50.0% | |
| GTCTTGACTGGCATGAGAGT+TGG | + | chr3.1:85894103-85894122 | MS.gene08041:CDS | 50.0% | |
| TTGACTGGCATGAGAGTTGG+TGG | + | chr3.1:85894106-85894125 | MS.gene08041:CDS | 50.0% | |
| ACTTCCTCTGGATGAGGACC+AGG | + | chr3.1:85893411-85893430 | MS.gene08041:CDS | 55.0% | |
| CTAACCTGGTCCTCATCCAG+AGG | - | chr3.1:85893418-85893437 | None:intergenic | 55.0% | |
| GAGAAAGAAGGCTGCGTCGA+GGG | - | chr3.1:85894607-85894626 | None:intergenic | 55.0% | |
| TGAGAAAGAAGGCTGCGTCG+AGG | - | chr3.1:85894608-85894627 | None:intergenic | 55.0% | |
| TGGGGTTAGATGCGGCCTTG+CGG | + | chr3.1:85893618-85893637 | MS.gene08041:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 85893340 | 85894703 | 85893340 | ID=MS.gene08041 |
| chr3.1 | mRNA | 85893340 | 85894703 | 85893340 | ID=MS.gene08041.t1;Parent=MS.gene08041 |
| chr3.1 | exon | 85893340 | 85893432 | 85893340 | ID=MS.gene08041.t1.exon1;Parent=MS.gene08041.t1 |
| chr3.1 | CDS | 85893340 | 85893432 | 85893340 | ID=cds.MS.gene08041.t1;Parent=MS.gene08041.t1 |
| chr3.1 | exon | 85894082 | 85894127 | 85894082 | ID=MS.gene08041.t1.exon2;Parent=MS.gene08041.t1 |
| chr3.1 | CDS | 85894082 | 85894127 | 85894082 | ID=cds.MS.gene08041.t1;Parent=MS.gene08041.t1 |
| chr3.1 | exon | 85894290 | 85894360 | 85894290 | ID=MS.gene08041.t1.exon3;Parent=MS.gene08041.t1 |
| chr3.1 | CDS | 85894290 | 85894360 | 85894290 | ID=cds.MS.gene08041.t1;Parent=MS.gene08041.t1 |
| chr3.1 | exon | 85894599 | 85894703 | 85894599 | ID=MS.gene08041.t1.exon4;Parent=MS.gene08041.t1 |
| chr3.1 | CDS | 85894599 | 85894703 | 85894599 | ID=cds.MS.gene08041.t1;Parent=MS.gene08041.t1 |
| Gene Sequence |
| Protein sequence |
