AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20808

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20808.t1 MTR_1g089180 94.397 464 26 0 1 464 1 464 0.0 913
MS.gene20808.t1 MTR_6g027840 30.263 456 288 9 1 429 1 453 3.89e-54 190
MS.gene20808.t1 MTR_2g045090 86.567 67 9 0 183 249 9 75 7.99e-32 119
MS.gene20808.t1 MTR_5g043640 31.694 183 101 2 250 429 51 212 1.31e-18 85.5
MS.gene20808.t1 MTR_5g043640 30.729 192 110 3 220 410 57 226 1.26e-15 77.0
MS.gene20808.t1 MTR_8g056020 25.714 350 212 10 1 306 3 348 8.76e-16 79.0
MS.gene20808.t1 MTR_2g096760 31.250 224 137 8 211 429 7 218 1.56e-14 75.9
MS.gene20808.t1 MTR_1g112830 27.933 179 109 2 257 435 173 331 2.02e-14 75.9
MS.gene20808.t1 MTR_2g006870 27.692 195 121 3 228 402 501 695 7.36e-14 74.3
MS.gene20808.t1 MTR_4g062100 28.016 257 151 11 183 429 66 298 1.30e-13 73.2
MS.gene20808.t1 MTR_2g096190 25.704 284 173 10 161 429 34 294 1.36e-13 73.2
MS.gene20808.t1 MTR_4g063830 28.384 229 149 5 206 429 1 219 1.86e-13 72.4
MS.gene20808.t1 MTR_4g116160 28.235 170 102 2 260 429 77 226 2.19e-13 72.4
MS.gene20808.t1 MTR_4g129470 27.826 230 141 6 221 429 20 245 3.25e-13 72.0
MS.gene20808.t1 MTR_1g100797 28.500 200 116 5 255 429 47 244 4.42e-13 71.6
MS.gene20808.t1 MTR_4g116160 28.235 170 102 2 260 429 77 226 5.42e-13 71.6
MS.gene20808.t1 MTR_7g100430 28.512 242 135 7 213 444 119 332 5.64e-13 71.2
MS.gene20808.t1 MTR_0020s0130 27.803 223 131 8 233 429 26 244 9.66e-13 70.5
MS.gene20808.t1 MTR_4g021880 26.457 223 149 5 220 437 100 312 2.16e-12 68.9
MS.gene20808.t1 MTR_4g021880 29.858 211 129 5 221 425 136 333 1.91e-11 66.2
MS.gene20808.t1 MTR_4g092760 29.808 208 108 5 268 458 72 258 2.25e-12 69.3
MS.gene20808.t1 MTR_4g021880 26.457 223 149 5 220 437 100 312 3.98e-12 68.6
MS.gene20808.t1 MTR_4g021880 30.332 211 128 7 221 425 136 333 1.37e-11 67.0
MS.gene20808.t1 MTR_3g088410 36.735 98 62 0 342 439 22 119 1.07e-11 63.2
MS.gene20808.t1 MTR_7g117620 31.551 187 109 6 249 417 112 297 2.41e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20808.t1 AT2G03430 31.551 187 104 2 246 429 40 205 4.40e-17 80.9
MS.gene20808.t1 AT2G03430 28.571 231 114 5 180 410 40 219 1.80e-14 73.6
MS.gene20808.t1 AT5G60070 28.309 272 153 8 194 429 39 304 1.25e-16 82.8
MS.gene20808.t1 AT2G14255 30.682 176 101 3 260 435 65 219 2.28e-15 79.0
MS.gene20808.t1 AT2G14255 25.581 215 133 4 214 425 55 245 4.40e-14 74.7
MS.gene20808.t1 AT5G20350 30.000 170 99 2 260 429 72 221 1.48e-14 76.6
MS.gene20808.t1 AT2G28840 27.500 240 120 7 200 430 25 219 1.82e-14 75.9
MS.gene20808.t1 AT2G31820 29.520 271 159 10 187 429 151 417 3.11e-14 75.5
MS.gene20808.t1 AT2G31820 28.151 238 149 9 218 434 152 388 1.67e-12 70.1
MS.gene20808.t1 AT1G07710 27.734 256 153 9 183 429 62 294 3.98e-14 75.1
MS.gene20808.t1 AT1G07710 27.734 256 153 9 183 429 62 294 3.98e-14 75.1
MS.gene20808.t1 AT1G05640 31.081 296 168 13 167 429 89 381 7.54e-14 74.3
MS.gene20808.t1 AT5G57740 29.412 204 119 6 255 437 53 252 2.36e-13 72.4
MS.gene20808.t1 AT3G04710 33.149 181 95 6 257 435 283 439 6.97e-13 71.2
MS.gene20808.t1 AT3G04710 33.149 181 95 6 257 435 59 215 1.10e-12 70.1
MS.gene20808.t1 AT3G04710 33.149 181 95 6 257 435 59 215 1.18e-12 70.1
MS.gene20808.t1 AT5G02620 27.801 241 139 8 194 421 24 242 1.34e-12 70.1
MS.gene20808.t1 AT5G02620 27.801 241 139 8 194 421 24 242 1.34e-12 70.1
MS.gene20808.t1 AT5G02620 26.384 307 183 12 161 449 24 305 7.09e-12 67.8
MS.gene20808.t1 AT5G02620 26.384 307 183 12 161 449 24 305 7.09e-12 67.8
MS.gene20808.t1 AT5G02620 26.384 307 183 12 161 449 24 305 7.09e-12 67.8
MS.gene20808.t1 AT2G26650 29.714 175 100 3 244 418 437 588 1.83e-11 67.0
MS.gene20808.t1 AT2G26650 28.571 175 102 2 244 418 542 693 2.06e-11 67.0
MS.gene20808.t1 AT5G07270 28.643 199 111 5 255 429 47 238 3.68e-11 65.5
MS.gene20808.t1 AT5G14230 27.778 306 186 12 153 429 303 602 4.69e-11 65.5
MS.gene20808.t1 AT5G12320 31.731 104 71 0 336 439 7 110 5.48e-11 60.8
MS.gene20808.t1 AT5G14230 27.778 306 186 12 153 429 143 442 7.55e-11 64.7

Find 91 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGACAAAGGTGGTTGATTA+AGG 0.254768 1.2:+67041693 None:intergenic
CTGAAGCTTCTTGCAGCTAA+TGG 0.286643 1.2:-67041302 MS.gene20808:CDS
CTTGTTTGGTGTACTACATT+GGG 0.316912 1.2:-67040910 MS.gene20808:CDS
ATCGTCTGTAGTGGCTTTAC+CGG 0.321963 1.2:+67041628 None:intergenic
TGAATCTTTCAGCGACGAAT+TGG 0.365290 1.2:-67041198 MS.gene20808:CDS
ATTCCGTTTGCAATAAGAAC+AGG 0.367201 1.2:-67041338 MS.gene20808:CDS
CACGCGCTGAAGAAGTGTTT+AGG 0.372386 1.2:-67040849 MS.gene20808:CDS
TTGCATTGTGCTGCTGAAGC+TGG 0.388322 1.2:-67040681 MS.gene20808:CDS
AGTGGTCACACGCGCTTGTT+TGG 0.388536 1.2:-67040924 MS.gene20808:CDS
TGCAGTTATAACCGTCGTTT+TGG 0.395549 1.2:-67041561 MS.gene20808:CDS
TGTTGGGTTTGTTGTTGTTA+AGG 0.397778 1.2:+67041790 None:intergenic
GCTTGTTTGGTGTACTACAT+TGG 0.398911 1.2:-67040911 MS.gene20808:CDS
TCACTCGCTGCCACGTGAAC+TGG 0.399901 1.2:+67041143 None:intergenic
CGACGAATTGGATGTGAATT+CGG 0.401629 1.2:-67041186 MS.gene20808:CDS
GAGTGGTGGGATTACTGATA+AGG 0.420907 1.2:+67041748 None:intergenic
TTGTTGATTGAAAAGGTTGT+TGG 0.422808 1.2:+67041773 None:intergenic
TTCCCTGTTCTTATTGCAAA+CGG 0.423986 1.2:+67041335 None:intergenic
AGTGGTGGGATTACTGATAA+GGG 0.424185 1.2:+67041749 None:intergenic
ACGCGCTGAAGAAGTGTTTA+GGG 0.432430 1.2:-67040848 MS.gene20808:CDS
TGTTGATTGAAAAGGTTGTT+GGG 0.435252 1.2:+67041774 None:intergenic
CTACATTGGGCTTCATTCAA+AGG 0.464048 1.2:-67040780 MS.gene20808:CDS
TAAGGGTTTGTTGATTGAAA+AGG 0.465974 1.2:+67041766 None:intergenic
TGAAACTCGCGGTTGAAAGC+GGG 0.467803 1.2:-67041436 MS.gene20808:CDS
ATGAAACTCGCGGTTGAAAG+CGG 0.472267 1.2:-67041437 MS.gene20808:CDS
TGGGTGGAGCCCACTACATT+GGG 0.483776 1.2:-67040793 MS.gene20808:CDS
TTGAATGAAGCCCAATGTAG+TGG 0.486452 1.2:+67040783 None:intergenic
TACATTGGGCTTCATTCAAA+GGG 0.487037 1.2:-67040779 MS.gene20808:CDS
GGCTTTACCGGTTGGTAACA+TGG 0.487305 1.2:+67041640 None:intergenic
TGGACATTTACAAGTTGCAT+TGG 0.499069 1.2:-67040661 MS.gene20808:CDS
CGTCGTCTCTTTAACAAACC+TGG 0.511564 1.2:-67041602 MS.gene20808:CDS
TCTGTAGTGGCTTTACCGGT+TGG 0.516796 1.2:+67041632 None:intergenic
ATGGGTGGAGCCCACTACAT+TGG 0.517641 1.2:-67040794 MS.gene20808:CDS
CTGAAGAAGTGTTTAGGGGA+AGG 0.519433 1.2:-67040843 MS.gene20808:CDS
GTTGATAGTGTTGATGATGC+TGG 0.521848 1.2:-67040714 MS.gene20808:CDS
TTTGCACGAAGCTGCAACAA+TGG 0.526496 1.2:-67041249 MS.gene20808:CDS
TCACGGAAAACGTGGGGACC+AGG 0.526974 1.2:+67041584 None:intergenic
GAAGAAGTGTTTAGGGGAAG+GGG 0.527190 1.2:-67040841 MS.gene20808:CDS
TGAATAGTAAAGATCAGAAT+GGG 0.527705 1.2:-67040812 MS.gene20808:CDS
CGAACGGTGATGACGTCAGC+AGG 0.533305 1.2:+67041668 None:intergenic
CGAACCTCCATGTTACCAAC+CGG 0.541438 1.2:-67041647 MS.gene20808:CDS
ACCGATCTTATCGCCGCCGG+TGG 0.542266 1.2:-67041398 MS.gene20808:CDS
CATCACCACCATGGATAGGT+TGG 0.547063 1.2:-67041918 None:intergenic
GTGAATAGTAAAGATCAGAA+TGG 0.548627 1.2:-67040813 MS.gene20808:CDS
TGAAGAAGTGTTTAGGGGAA+GGG 0.558826 1.2:-67040842 MS.gene20808:CDS
TTCGACGCTGCATGATGAAG+TGG 0.565036 1.2:+67041044 None:intergenic
AAATACGCAAGAGAAATAAA+CGG 0.568319 1.2:-67040975 MS.gene20808:CDS
TCTAAAATTCACATCTCCAC+CGG 0.573420 1.2:+67041382 None:intergenic
TAACTGCATCACGGAAAACG+TGG 0.574738 1.2:+67041576 None:intergenic
GAAGTGTTTAGGGGAAGGGG+CGG 0.580140 1.2:-67040838 MS.gene20808:CDS
TCCACCGGCGGCGATAAGAT+CGG 0.585692 1.2:+67041397 None:intergenic
TAACCATCACCACCATGGAT+AGG 0.591904 1.2:-67041922 None:intergenic
TTCCGTTTGCAATAAGAACA+GGG 0.592729 1.2:-67041337 MS.gene20808:CDS
ATGTTGATGAAGAGAGTGGT+GGG 0.599092 1.2:+67041735 None:intergenic
TTTGTTGGAACATGGTGCTG+AGG 0.600880 1.2:-67040736 MS.gene20808:CDS
TTGTTTGGTGTACTACATTG+GGG 0.602036 1.2:-67040909 MS.gene20808:CDS
GTTCACGTGGCAGCGAGTGA+AGG 0.604991 1.2:-67041140 MS.gene20808:CDS
GCTTCTACGAGCGGCGCGTG+TGG 0.605091 1.2:-67040880 MS.gene20808:CDS
TGAATGAAGCCCAATGTAGT+GGG 0.611394 1.2:+67040784 None:intergenic
AACAGCGTTTGAAATAGCAT+CGG 0.612401 1.2:-67040949 MS.gene20808:CDS
GCTTCAGCAGCACAATGCAA+AGG 0.613566 1.2:+67040684 None:intergenic
GATAACAAACTCAGCGACGG+TGG 0.615331 1.2:+67041532 None:intergenic
AACTGCATCACGGAAAACGT+GGG 0.618543 1.2:+67041577 None:intergenic
TGGTAACATGGAGGTTCGAA+CGG 0.618785 1.2:+67041652 None:intergenic
TGTCCACCGTCGAGAATCAA+CGG 0.621480 1.2:+67041067 None:intergenic
GGGCGACGCGCTTCTACGAG+CGG 0.623068 1.2:-67040889 MS.gene20808:CDS
GTCACCGATCTTATCGCCGC+CGG 0.623931 1.2:-67041401 MS.gene20808:CDS
ACGTCAGCAGGTTCAGACAA+AGG 0.630951 1.2:+67041680 None:intergenic
TTTGAAATAGCATCGGAGAG+TGG 0.632198 1.2:-67040942 MS.gene20808:CDS
ATGCAGAATCAACGATTCAG+TGG 0.632543 1.2:-67041279 MS.gene20808:CDS
AGGGAGAATAAAGAGTGTCA+AGG 0.637144 1.2:-67040760 MS.gene20808:CDS
CGCGCTGAAGAAGTGTTTAG+GGG 0.639372 1.2:-67040847 MS.gene20808:CDS
ATAGTAAAGATCAGAATGGG+TGG 0.645517 1.2:-67040809 MS.gene20808:CDS
ACGACGGAGATCGTCTGTAG+TGG 0.650285 1.2:+67041619 None:intergenic
TAAGGAAACTGCAACAGACA+TGG 0.651639 1.2:+67041808 None:intergenic
GAGGTTTGTGAGCTTGAACG+AGG 0.657050 1.2:+67041838 None:intergenic
CAGGTTTGTTAAAGAGACGA+CGG 0.657239 1.2:+67041603 None:intergenic
AATGATGACACCAGTTCACG+TGG 0.662960 1.2:-67041153 MS.gene20808:CDS
CACGAATTTGATGAAACTCG+CGG 0.663191 1.2:-67041447 MS.gene20808:CDS
ATAACAAACTCAGCGACGGT+GGG 0.664181 1.2:+67041533 None:intergenic
CAACAGACATGGTGTGCATG+AGG 0.670248 1.2:+67041819 None:intergenic
AAAATTCACATCTCCACCGG+CGG 0.675896 1.2:+67041385 None:intergenic
CTAACGCCGTTGATTCTCGA+CGG 0.675903 1.2:-67041073 MS.gene20808:CDS
TGAGATAACAAACTCAGCGA+CGG 0.679630 1.2:+67041529 None:intergenic
TATGTTGATGAAGAGAGTGG+TGG 0.683276 1.2:+67041734 None:intergenic
GTGTATGTTGATGAAGAGAG+TGG 0.692828 1.2:+67041731 None:intergenic
ACTGCATCACGGAAAACGTG+GGG 0.694469 1.2:+67041578 None:intergenic
CGACGGTTATAACTGCATCA+CGG 0.697949 1.2:+67041567 None:intergenic
ACGCCGTTGATTCTCGACGG+TGG 0.707122 1.2:-67041070 MS.gene20808:CDS
TTTACCGGTTGGTAACATGG+AGG 0.712639 1.2:+67041643 None:intergenic
GGTGGGACCTACCAAAACGA+CGG 0.719809 1.2:+67041550 None:intergenic
TCAGCAGGTTCAGACAAAGG+TGG 0.732780 1.2:+67041683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATACGCAAGAGAAATAAA+CGG - chr1.2:67041469-67041488 MS.gene20808:CDS 25.0%
! GTCGAAAAATCATTTAAAAG+CGG - chr1.2:67041417-67041436 MS.gene20808:CDS 25.0%
! TGAATAGTAAAGATCAGAAT+GGG - chr1.2:67041632-67041651 MS.gene20808:CDS 25.0%
!! ATTGAGTTTTTCGTTTCAAT+CGG - chr1.2:67041340-67041359 MS.gene20808:CDS 25.0%
!!! AACAACATTTTTGGTTTTTC+TGG + chr1.2:67040586-67040605 None:intergenic 25.0%
!!! GTGAATTTTAGAGATTCAAA+CGG - chr1.2:67041073-67041092 MS.gene20808:CDS 25.0%
GTGAATAGTAAAGATCAGAA+TGG - chr1.2:67041631-67041650 MS.gene20808:CDS 30.0%
! TCTTTTCACGAAAGCTATAT+CGG - chr1.2:67040955-67040974 MS.gene20808:CDS 30.0%
! TGTTGATTGAAAAGGTTGTT+GGG + chr1.2:67040673-67040692 None:intergenic 30.0%
! TTGTTGATTGAAAAGGTTGT+TGG + chr1.2:67040674-67040693 None:intergenic 30.0%
!! TAAGGGTTTGTTGATTGAAA+AGG + chr1.2:67040681-67040700 None:intergenic 30.0%
!! TGCTTTTGAAAAGAAGCAAT+TGG + chr1.2:67041865-67041884 None:intergenic 30.0%
!!! ACATTTTTGGTTTTTCTGGA+AGG + chr1.2:67040582-67040601 None:intergenic 30.0%
!!! CATTTTTGGTTTTTCTGGAA+GGG + chr1.2:67040581-67040600 None:intergenic 30.0%
ATAGTAAAGATCAGAATGGG+TGG - chr1.2:67041635-67041654 MS.gene20808:CDS 35.0%
ATTCCGTTTGCAATAAGAAC+AGG - chr1.2:67041106-67041125 MS.gene20808:CDS 35.0%
TACATTGGGCTTCATTCAAA+GGG - chr1.2:67041665-67041684 MS.gene20808:CDS 35.0%
TGGACATTTACAAGTTGCAT+TGG - chr1.2:67041783-67041802 MS.gene20808:CDS 35.0%
TTCCCTGTTCTTATTGCAAA+CGG + chr1.2:67041112-67041131 None:intergenic 35.0%
TTCCGTTTGCAATAAGAACA+GGG - chr1.2:67041107-67041126 MS.gene20808:CDS 35.0%
! AACAGCGTTTGAAATAGCAT+CGG - chr1.2:67041495-67041514 MS.gene20808:CDS 35.0%
! TCTAAAATTCACATCTCCAC+CGG + chr1.2:67041065-67041084 None:intergenic 35.0%
! TGAACGAGGAACAACATTTT+TGG + chr1.2:67040595-67040614 None:intergenic 35.0%
! TTCTCTTGCGTATTTTACGT+CGG + chr1.2:67041464-67041483 None:intergenic 35.0%
!! CTTGTTTGGTGTACTACATT+GGG - chr1.2:67041534-67041553 MS.gene20808:CDS 35.0%
!! TGTTGGGTTTGTTGTTGTTA+AGG + chr1.2:67040657-67040676 None:intergenic 35.0%
!! TTGTTTGGTGTACTACATTG+GGG - chr1.2:67041535-67041554 MS.gene20808:CDS 35.0%
!!! AAAGAGTGTCAAGGTTTTGT+TGG - chr1.2:67041693-67041712 MS.gene20808:CDS 35.0%
ATGCAGAATCAACGATTCAG+TGG - chr1.2:67041165-67041184 MS.gene20808:CDS 40.0%
CACGAATTTGATGAAACTCG+CGG - chr1.2:67040997-67041016 MS.gene20808:CDS 40.0%
CAGACAAAGGTGGTTGATTA+AGG + chr1.2:67040754-67040773 None:intergenic 40.0%
CGACGAATTGGATGTGAATT+CGG - chr1.2:67041258-67041277 MS.gene20808:CDS 40.0%
CTACATTGGGCTTCATTCAA+AGG - chr1.2:67041664-67041683 MS.gene20808:CDS 40.0%
TAAGGAAACTGCAACAGACA+TGG + chr1.2:67040639-67040658 None:intergenic 40.0%
TGAATCTTTCAGCGACGAAT+TGG - chr1.2:67041246-67041265 MS.gene20808:CDS 40.0%
TGAATGAAGCCCAATGTAGT+GGG + chr1.2:67041663-67041682 None:intergenic 40.0%
TGAGATAACAAACTCAGCGA+CGG + chr1.2:67040918-67040937 None:intergenic 40.0%
TTGAATGAAGCCCAATGTAG+TGG + chr1.2:67041664-67041683 None:intergenic 40.0%
! AGTGGTGGGATTACTGATAA+GGG + chr1.2:67040698-67040717 None:intergenic 40.0%
! ATGTTGATGAAGAGAGTGGT+GGG + chr1.2:67040712-67040731 None:intergenic 40.0%
! GAGTTTTTCGTTTCAATCGG+AGG - chr1.2:67041343-67041362 MS.gene20808:CDS 40.0%
! GTGTATGTTGATGAAGAGAG+TGG + chr1.2:67040716-67040735 None:intergenic 40.0%
! TATGTTGATGAAGAGAGTGG+TGG + chr1.2:67040713-67040732 None:intergenic 40.0%
! TGCAGTTATAACCGTCGTTT+TGG - chr1.2:67040883-67040902 MS.gene20808:CDS 40.0%
! TTTGAAATAGCATCGGAGAG+TGG - chr1.2:67041502-67041521 MS.gene20808:CDS 40.0%
!! AGGGAGAATAAAGAGTGTCA+AGG - chr1.2:67041684-67041703 MS.gene20808:CDS 40.0%
!! CAGGTTTGTTAAAGAGACGA+CGG + chr1.2:67040844-67040863 None:intergenic 40.0%
!! GCTTGTTTGGTGTACTACAT+TGG - chr1.2:67041533-67041552 MS.gene20808:CDS 40.0%
!! GTTATAACCGTCGTTTTGGT+AGG - chr1.2:67040887-67040906 MS.gene20808:CDS 40.0%
!! GTTGATAGTGTTGATGATGC+TGG - chr1.2:67041730-67041749 MS.gene20808:CDS 40.0%
!! TGAAGAAGTGTTTAGGGGAA+GGG - chr1.2:67041602-67041621 MS.gene20808:CDS 40.0%
!!! GCATTGGTTTTGATTGCTCA+TGG - chr1.2:67041799-67041818 MS.gene20808:CDS 40.0%
!!! GTCAAGGTTTTGTTGGAACA+TGG - chr1.2:67041700-67041719 MS.gene20808:CDS 40.0%
!!! TTGGTTTTGATTGCTCATGG+TGG - chr1.2:67041802-67041821 MS.gene20808:CDS 40.0%
!!! TTTGGTTTTTCTGGAAGGGA+AGG + chr1.2:67040577-67040596 None:intergenic 40.0%
AAAATTCACATCTCCACCGG+CGG + chr1.2:67041062-67041081 None:intergenic 45.0%
AACTGCATCACGGAAAACGT+GGG + chr1.2:67040870-67040889 None:intergenic 45.0%
AATGATGACACCAGTTCACG+TGG - chr1.2:67041291-67041310 MS.gene20808:CDS 45.0%
ATAACAAACTCAGCGACGGT+GGG + chr1.2:67040914-67040933 None:intergenic 45.0%
ATGAAACTCGCGGTTGAAAG+CGG - chr1.2:67041007-67041026 MS.gene20808:CDS 45.0%
CGACGGTTATAACTGCATCA+CGG + chr1.2:67040880-67040899 None:intergenic 45.0%
CGTCGTCTCTTTAACAAACC+TGG - chr1.2:67040842-67040861 MS.gene20808:CDS 45.0%
CTGAAGCTTCTTGCAGCTAA+TGG - chr1.2:67041142-67041161 MS.gene20808:CDS 45.0%
TAACTGCATCACGGAAAACG+TGG + chr1.2:67040871-67040890 None:intergenic 45.0%
TGGTAACATGGAGGTTCGAA+CGG + chr1.2:67040795-67040814 None:intergenic 45.0%
TTTGCACGAAGCTGCAACAA+TGG - chr1.2:67041195-67041214 MS.gene20808:CDS 45.0%
! ATCGTCTGTAGTGGCTTTAC+CGG + chr1.2:67040819-67040838 None:intergenic 45.0%
! GAGTGGTGGGATTACTGATA+AGG + chr1.2:67040699-67040718 None:intergenic 45.0%
! TTTGTTGGAACATGGTGCTG+AGG - chr1.2:67041708-67041727 MS.gene20808:CDS 45.0%
!! ACGCGCTGAAGAAGTGTTTA+GGG - chr1.2:67041596-67041615 MS.gene20808:CDS 45.0%
!! CTGAAGAAGTGTTTAGGGGA+AGG - chr1.2:67041601-67041620 MS.gene20808:CDS 45.0%
!! GAAGAAGTGTTTAGGGGAAG+GGG - chr1.2:67041603-67041622 MS.gene20808:CDS 45.0%
!! TTTACCGGTTGGTAACATGG+AGG + chr1.2:67040804-67040823 None:intergenic 45.0%
ACGTCAGCAGGTTCAGACAA+AGG + chr1.2:67040767-67040786 None:intergenic 50.0%
ACTGCATCACGGAAAACGTG+GGG + chr1.2:67040869-67040888 None:intergenic 50.0%
CAACAGACATGGTGTGCATG+AGG + chr1.2:67040628-67040647 None:intergenic 50.0%
CGAACCTCCATGTTACCAAC+CGG - chr1.2:67040797-67040816 MS.gene20808:CDS 50.0%
GAGGTTTGTGAGCTTGAACG+AGG + chr1.2:67040609-67040628 None:intergenic 50.0%
GATAACAAACTCAGCGACGG+TGG + chr1.2:67040915-67040934 None:intergenic 50.0%
GCTTCAGCAGCACAATGCAA+AGG + chr1.2:67041763-67041782 None:intergenic 50.0%
TCAGCAGGTTCAGACAAAGG+TGG + chr1.2:67040764-67040783 None:intergenic 50.0%
TGAAACTCGCGGTTGAAAGC+GGG - chr1.2:67041008-67041027 MS.gene20808:CDS 50.0%
TGTCCACCGTCGAGAATCAA+CGG + chr1.2:67041380-67041399 None:intergenic 50.0%
TTCGACGCTGCATGATGAAG+TGG + chr1.2:67041403-67041422 None:intergenic 50.0%
! TCTGTAGTGGCTTTACCGGT+TGG + chr1.2:67040815-67040834 None:intergenic 50.0%
! TTGCATTGTGCTGCTGAAGC+TGG - chr1.2:67041763-67041782 MS.gene20808:CDS 50.0%
!! CACGCGCTGAAGAAGTGTTT+AGG - chr1.2:67041595-67041614 MS.gene20808:CDS 50.0%
!! CGCGCTGAAGAAGTGTTTAG+GGG - chr1.2:67041597-67041616 MS.gene20808:CDS 50.0%
!! CTAACGCCGTTGATTCTCGA+CGG - chr1.2:67041371-67041390 MS.gene20808:CDS 50.0%
!! GGCTTTACCGGTTGGTAACA+TGG + chr1.2:67040807-67040826 None:intergenic 50.0%
ACGACGGAGATCGTCTGTAG+TGG + chr1.2:67040828-67040847 None:intergenic 55.0%
AGTGGTCACACGCGCTTGTT+TGG - chr1.2:67041520-67041539 MS.gene20808:CDS 55.0%
ATGGGTGGAGCCCACTACAT+TGG - chr1.2:67041650-67041669 MS.gene20808:CDS 55.0%
GGTGGGACCTACCAAAACGA+CGG + chr1.2:67040897-67040916 None:intergenic 55.0%
TGGGTGGAGCCCACTACATT+GGG - chr1.2:67041651-67041670 MS.gene20808:CDS 55.0%
! GAAGTGTTTAGGGGAAGGGG+CGG - chr1.2:67041606-67041625 MS.gene20808:CDS 55.0%
GTCACCGATCTTATCGCCGC+CGG - chr1.2:67041043-67041062 MS.gene20808:CDS 60.0%
GTTCACGTGGCAGCGAGTGA+AGG - chr1.2:67041304-67041323 MS.gene20808:CDS 60.0%
TCACGGAAAACGTGGGGACC+AGG + chr1.2:67040863-67040882 None:intergenic 60.0%
TCACTCGCTGCCACGTGAAC+TGG + chr1.2:67041304-67041323 None:intergenic 60.0%
TCCACCGGCGGCGATAAGAT+CGG + chr1.2:67041050-67041069 None:intergenic 60.0%
! CGAACGGTGATGACGTCAGC+AGG + chr1.2:67040779-67040798 None:intergenic 60.0%
!! ACGCCGTTGATTCTCGACGG+TGG - chr1.2:67041374-67041393 MS.gene20808:CDS 60.0%
ACCGATCTTATCGCCGCCGG+TGG - chr1.2:67041046-67041065 MS.gene20808:CDS 65.0%
GCTTCTACGAGCGGCGCGTG+TGG - chr1.2:67041564-67041583 MS.gene20808:CDS 70.0%
GGGCGACGCGCTTCTACGAG+CGG - chr1.2:67041555-67041574 MS.gene20808:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 67040536 67041930 67040536 ID=MS.gene20808
chr1.2 mRNA 67040536 67041930 67040536 ID=MS.gene20808.t1;Parent=MS.gene20808
chr1.2 exon 67040536 67041930 67040536 ID=MS.gene20808.t1.exon1;Parent=MS.gene20808.t1
chr1.2 CDS 67040536 67041930 67040536 ID=cds.MS.gene20808.t1;Parent=MS.gene20808.t1
Gene Sequence

>MS.gene20808

ATGGATAGGTTGGTGAAAACAGAGTTCAACGAAGTGAACCTTCCCTTCCAGAAAAACCAAAAATGTTGTTCCTCGTTCAAGCTCACAAACCTCATGCACACCATGTCTGTTGCAGTTTCCTTAACAACAACAAACCCAACAACCTTTTCAATCAACAAACCCTTATCAGTAATCCCACCACTCTCTTCATCAACATACACACTTCATCTCACAAACCTTAATCAACCACCTTTGTCTGAACCTGCTGACGTCATCACCGTTCGAACCTCCATGTTACCAACCGGTAAAGCCACTACAGACGATCTCCGTCGTCTCTTTAACAAACCTGGTCCCCACGTTTTCCGTGATGCAGTTATAACCGTCGTTTTGGTAGGTCCCACCGTCGCTGAGTTTGTTATCTCAAATTACGAAACTCGTAATCTTTTCACGAAAGCTATATCGGTTTGCACGAAATTAAATCTCACGAATTTGATGAAACTCGCGGTTGAAAGCGGGAAAGTTGAATACGTCACCGATCTTATCGCCGCCGGTGGAGATGTGAATTTTAGAGATTCAAACGGTAAATCGCTTATTCCGTTTGCAATAAGAACAGGGAAACTCGATATTCTGAAGCTTCTTGCAGCTAATGGATGCAGAATCAACGATTCAGTGGATTTTGTTTTGCACGAAGCTGCAACAATGGATAGAATCGATGTTGTAAAGTTTTTGCTTGAATCTTTCAGCGACGAATTGGATGTGAATTCGGTGAACAGAGAAATGATGACACCAGTTCACGTGGCAGCGAGTGAAGGTCACGTGAGTATAATTGAGTTTTTCGTTTCAATCGGAGGAAACGCTAACGCCGTTGATTCTCGACGGTGGACACCACTTCATCATGCAGCGTCGAAAAATCATTTAAAAGCGGTTGAGTTTTTACTACAAAACTCCGACGTAAAATACGCAAGAGAAATAAACGGTAAAACAGCGTTTGAAATAGCATCGGAGAGTGGTCACACGCGCTTGTTTGGTGTACTACATTGGGGCGACGCGCTTCTACGAGCGGCGCGTGTGGACGACGTACACGCGCTGAAGAAGTGTTTAGGGGAAGGGGCGGAAGTGAATAGTAAAGATCAGAATGGGTGGAGCCCACTACATTGGGCTTCATTCAAAGGGAGAATAAAGAGTGTCAAGGTTTTGTTGGAACATGGTGCTGAGGTTGATAGTGTTGATGATGCTGGTTACACTCCTTTGCATTGTGCTGCTGAAGCTGGACATTTACAAGTTGCATTGGTTTTGATTGCTCATGGTGGTTGTCAAACTAATCTTAAAAGTTTTCAACATGTTTCTCCAATTGCTTCTTTTCAAAAGCACGTTTCTCTTCATTACAGTACTAAAAAATCTGAGACATTTGCTTGA
Protein sequence

>MS.gene20808.t1

MDRLVKTEFNEVNLPFQKNQKCCSSFKLTNLMHTMSVAVSLTTTNPTTFSINKPLSVIPPLSSSTYTLHLTNLNQPPLSEPADVITVRTSMLPTGKATTDDLRRLFNKPGPHVFRDAVITVVLVGPTVAEFVISNYETRNLFTKAISVCTKLNLTNLMKLAVESGKVEYVTDLIAAGGDVNFRDSNGKSLIPFAIRTGKLDILKLLAANGCRINDSVDFVLHEAATMDRIDVVKFLLESFSDELDVNSVNREMMTPVHVAASEGHVSIIEFFVSIGGNANAVDSRRWTPLHHAASKNHLKAVEFLLQNSDVKYAREINGKTAFEIASESGHTRLFGVLHWGDALLRAARVDDVHALKKCLGEGAEVNSKDQNGWSPLHWASFKGRIKSVKVLLEHGAEVDSVDDAGYTPLHCAAEAGHLQVALVLIAHGGCQTNLKSFQHVSPIASFQKHVSLHYSTKKSETFA