AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21783


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21783.t1 MTR_5g096490 96.923 130 4 0 40 169 4 133 4.57e-88 254
MS.gene21783.t1 MTR_5g007240 92.086 139 10 1 32 169 28 166 7.95e-80 234
MS.gene21783.t1 MTR_5g096490 92.086 139 10 1 32 169 28 166 7.95e-80 234
MS.gene21783.t1 MTR_4g051390 42.975 121 67 1 50 168 1 121 6.29e-31 108
MS.gene21783.t1 MTR_1779s0020 42.975 121 67 1 50 168 1 121 8.99e-30 105
MS.gene21783.t1 MTR_2g087550 41.667 120 68 1 51 168 2 121 7.22e-28 103
MS.gene21783.t1 MTR_0002s0780 41.739 115 64 1 57 168 135 249 7.41e-24 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21783.t1 AT5G46160 78.030 132 22 1 45 169 42 173 5.79e-67 202
MS.gene21783.t1 AT5G46160 78.030 132 22 1 45 169 41 172 5.86e-67 202
MS.gene21783.t1 AT1G17560 60.131 153 32 3 45 168 43 195 5.42e-53 167
MS.gene21783.t1 AT1G17560 60.131 153 32 3 45 168 20 172 9.47e-53 166
MS.gene21783.t1 ATCG00780 44.628 121 65 1 50 168 1 121 1.79e-32 112

Find 40 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAGGTTGTGGACAATTC+TGG 0.240087 5.2:-1654273 MS.gene21783:CDS
GGACAATTCTGGAGCTAAAA+AGG 0.321429 5.2:-1654262 MS.gene21783:CDS
GACAAACAAGGGCAGCCTAT+TGG 0.362318 5.2:-1654039 MS.gene21783:CDS
ACAAACAAGGGCAGCCTATT+GGG 0.366691 5.2:-1654038 MS.gene21783:CDS
GTCAAGATCCTTACCTTAGC+TGG 0.374088 5.2:-1653967 MS.gene21783:CDS
GTGAAGGAAGCACATCCTAA+TGG 0.397241 5.2:-1654174 MS.gene21783:CDS
TGCCTCAACTCATGAGGAAC+TGG 0.420523 5.2:+1654000 None:intergenic
CCGAAAACTCTGGTCCCAAT+AGG 0.444490 5.2:+1654024 None:intergenic
CGCGCGTTGGCAGGGAAGAA+AGG 0.457182 5.2:-1654228 MS.gene21783:CDS
GGGAAGAAAGGAGCAAGATT+AGG 0.462937 5.2:-1654216 MS.gene21783:CDS
ATGTGCATACGCGCGTTGGC+AGG 0.463329 5.2:-1654237 MS.gene21783:CDS
CGTGTTTGACTTGTAAATAG+AGG 0.479650 5.2:+1654767 None:intergenic
TCCTAATGGAAAAGTGAAGA+AGG 0.493277 5.2:-1654160 MS.gene21783:CDS
CTGCCATCACACCTACCCTT+TGG 0.494271 5.2:+1654099 None:intergenic
TAAATAGAGGGAGTGATTAG+TGG 0.506799 5.2:+1654780 None:intergenic
AACAGTCCTCATCTGTATGA+AGG 0.520727 5.2:+1654296 None:intergenic
ATGCCAAAGGGTAGGTGTGA+TGG 0.533908 5.2:-1654102 MS.gene21783:CDS
TTAGTGGCACAAGTGCAAAC+TGG 0.534568 5.2:+1654796 None:intergenic
CAATATGCCCAGCTAAGGTA+AGG 0.539065 5.2:+1653959 None:intergenic
CATTGCTGCACGAACAACTA+CGG 0.550383 5.2:+1654122 None:intergenic
AGGAACTGGTCCGAAAACTC+TGG 0.550755 5.2:+1654014 None:intergenic
TCAAGATCCTTACCTTAGCT+GGG 0.562147 5.2:-1653966 MS.gene21783:CDS
CTATGCAATATGCCCAGCTA+AGG 0.565511 5.2:+1653954 None:intergenic
TGTTCGTGCAGCAATGCCAA+AGG 0.568662 5.2:-1654115 MS.gene21783:CDS
GTTAAAATGTCAACTTCCCC+AGG 0.577318 5.2:-1655056 MS.gene21783:intron
TGTGCATACGCGCGTTGGCA+GGG 0.580036 5.2:-1654236 MS.gene21783:CDS
GTGTTTGACTTGTAAATAGA+GGG 0.580442 5.2:+1654768 None:intergenic
GACCAGTTCCTCATGAGTTG+AGG 0.580661 5.2:-1654002 MS.gene21783:CDS
ACAGATGAGGACTGTTCTGA+AGG 0.580846 5.2:-1654289 MS.gene21783:CDS
GAGGACTGTTCTGAAGGTTG+TGG 0.589490 5.2:-1654283 MS.gene21783:CDS
GGTGATGTGCATACGCGCGT+TGG 0.592616 5.2:-1654241 MS.gene21783:CDS
GTTCGTGCAGCAATGCCAAA+GGG 0.599925 5.2:-1654114 MS.gene21783:CDS
CCTAATGGAAAAGTGAAGAA+GGG 0.626002 5.2:-1654159 MS.gene21783:CDS
TTCTTCTGCCTCAACTCATG+AGG 0.632900 5.2:+1653994 None:intergenic
GTGCAGCAATGCCAAAGGGT+AGG 0.641762 5.2:-1654110 MS.gene21783:CDS
GTTGTGCTTGTTGACAAACA+AGG 0.656177 5.2:-1654051 MS.gene21783:CDS
TATGATACAAGCATCAGTGA+AGG 0.662607 5.2:-1654190 MS.gene21783:CDS
AACGAACCTTCATACAGATG+AGG 0.663831 5.2:-1654302 MS.gene21783:CDS
TTGTGCTTGTTGACAAACAA+GGG 0.669343 5.2:-1654050 MS.gene21783:CDS
GGGTAGGTGTGATGGCAGTG+AGG 0.701393 5.2:-1654094 MS.gene21783:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGCATAAGAAAATAATGTTA+AGG + chr5.2:1654529-1654548 None:intergenic 20.0%
!! TAATATATGAAATTTCAGAG+TGG + chr5.2:1654191-1654210 None:intergenic 20.0%
!! TTCATACTTTCATTATTACA+TGG - chr5.2:1654090-1654109 MS.gene21783:CDS 20.0%
!!! CAAGAATTTTTATGTTGATA+AGG - chr5.2:1654287-1654306 MS.gene21783:CDS 20.0%
!!! GTTTTAGTAAACTAACAATT+TGG + chr5.2:1654059-1654078 None:intergenic 20.0%
!!! AAACATTGATTCTATCCTTT+TGG - chr5.2:1654024-1654043 MS.gene21783:CDS 25.0%
AAAACAGTTATTCTCTTACC+TGG + chr5.2:1653992-1654011 None:intergenic 30.0%
AAACAGTTATTCTCTTACCT+GGG + chr5.2:1653991-1654010 None:intergenic 30.0%
AAGATTACCAATACTGTGTT+TGG + chr5.2:1654554-1654573 None:intergenic 30.0%
! GAAAGTATGAAACTACAACA+TGG + chr5.2:1654081-1654100 None:intergenic 30.0%
! TATTTTTCTCAGGTACATGT+AGG - chr5.2:1654466-1654485 MS.gene21783:intron 30.0%
!! GTGTTTGACTTGTAAATAGA+GGG + chr5.2:1654262-1654281 None:intergenic 30.0%
!!! ACTGTTGTTTTCTACAATCT+AGG + chr5.2:1654580-1654599 None:intergenic 30.0%
!!! ATGCAACAGTTATTTTTCTC+AGG - chr5.2:1654456-1654475 MS.gene21783:intron 30.0%
!!! CATTTTAACTTCCGATGAAA+AGG + chr5.2:1653960-1653979 None:intergenic 30.0%
AACAGTTATTCTCTTACCTG+GGG + chr5.2:1653990-1654009 None:intergenic 35.0%
CCTAATGGAAAAGTGAAGAA+GGG - chr5.2:1654868-1654887 MS.gene21783:intron 35.0%
TAAATAGAGGGAGTGATTAG+TGG + chr5.2:1654250-1654269 None:intergenic 35.0%
TATGATACAAGCATCAGTGA+AGG - chr5.2:1654837-1654856 MS.gene21783:intron 35.0%
TCCTAATGGAAAAGTGAAGA+AGG - chr5.2:1654867-1654886 MS.gene21783:intron 35.0%
TGTGTGTAAGAAACTAAGCA+TGG + chr5.2:1654655-1654674 None:intergenic 35.0%
! AGACATTGTTTTCTTCAGGA+AGG + chr5.2:1654689-1654708 None:intergenic 35.0%
! AGGAAGACATTGTTTTCTTC+AGG + chr5.2:1654693-1654712 None:intergenic 35.0%
! TTGCCAATGCTCATGTTTTA+GGG + chr5.2:1654126-1654145 None:intergenic 35.0%
! TTGTGCTTGTTGACAAACAA+GGG - chr5.2:1654977-1654996 MS.gene21783:intron 35.0%
! TTTGCCAATGCTCATGTTTT+AGG + chr5.2:1654127-1654146 None:intergenic 35.0%
!! CGTGTTTGACTTGTAAATAG+AGG + chr5.2:1654263-1654282 None:intergenic 35.0%
!!! CATTGATTCTATCCTTTTGG+TGG - chr5.2:1654027-1654046 MS.gene21783:CDS 35.0%
AACAGTCCTCATCTGTATGA+AGG + chr5.2:1654734-1654753 None:intergenic 40.0%
AACGAACCTTCATACAGATG+AGG - chr5.2:1654725-1654744 MS.gene21783:intron 40.0%
ACATGTATCTTTGCACTGCA+TGG + chr5.2:1654610-1654629 None:intergenic 40.0%
AGAGTGGTTGCAAAATGTAG+TGG + chr5.2:1654175-1654194 None:intergenic 40.0%
ATGCTTGCCAAACACAGTAT+TGG - chr5.2:1654544-1654563 MS.gene21783:intron 40.0%
ATTTGGACATCACCACCAAA+AGG + chr5.2:1654042-1654061 None:intergenic 40.0%
GTTAAAATGTCAACTTCCCC+AGG - chr5.2:1653971-1653990 MS.gene21783:CDS 40.0%
TACATGTAGGAATCAGTTGC+AGG - chr5.2:1654479-1654498 MS.gene21783:intron 40.0%
TCAAGATCCTTACCTTAGCT+GGG - chr5.2:1655061-1655080 MS.gene21783:CDS 40.0%
TTAATCCACCGAACAGAGAA+CGG + chr5.2:1654393-1654412 None:intergenic 40.0%
! GTTGTGCTTGTTGACAAACA+AGG - chr5.2:1654976-1654995 MS.gene21783:intron 40.0%
!! CCCTTCTTCACTTTTCCATT+AGG + chr5.2:1654871-1654890 None:intergenic 40.0%
!! GGACAATTCTGGAGCTAAAA+AGG - chr5.2:1654765-1654784 MS.gene21783:CDS 40.0%
ACAAACAAGGGCAGCCTATT+GGG - chr5.2:1654989-1655008 MS.gene21783:intron 45.0%
ACAGATGAGGACTGTTCTGA+AGG - chr5.2:1654738-1654757 MS.gene21783:intron 45.0%
CAATATGCCCAGCTAAGGTA+AGG + chr5.2:1655071-1655090 None:intergenic 45.0%
CAGAGAACGGCTTACTAGTT+TGG + chr5.2:1654380-1654399 None:intergenic 45.0%
CATTGCTGCACGAACAACTA+CGG + chr5.2:1654908-1654927 None:intergenic 45.0%
CTAGTAAGCCGTTCTCTGTT+CGG - chr5.2:1654382-1654401 MS.gene21783:intron 45.0%
CTATGCAATATGCCCAGCTA+AGG + chr5.2:1655076-1655095 None:intergenic 45.0%
CTGAAGGTTGTGGACAATTC+TGG - chr5.2:1654754-1654773 MS.gene21783:CDS 45.0%
GCTCCCTAAAACATGAGCAT+TGG - chr5.2:1654120-1654139 MS.gene21783:CDS 45.0%
GGGAAGAAAGGAGCAAGATT+AGG - chr5.2:1654811-1654830 MS.gene21783:CDS 45.0%
GGTTCGTTGTTGCTGCAAAA+AGG + chr5.2:1654713-1654732 None:intergenic 45.0%
GTCAAGATCCTTACCTTAGC+TGG - chr5.2:1655060-1655079 MS.gene21783:CDS 45.0%
GTGACGTGGTAAATAGACCA+GGG + chr5.2:1654426-1654445 None:intergenic 45.0%
TGACGTGGTAAATAGACCAG+GGG + chr5.2:1654425-1654444 None:intergenic 45.0%
TGTGACGTGGTAAATAGACC+AGG + chr5.2:1654427-1654446 None:intergenic 45.0%
TTAGTGGCACAAGTGCAAAC+TGG + chr5.2:1654234-1654253 None:intergenic 45.0%
TTCTTCTGCCTCAACTCATG+AGG + chr5.2:1655036-1655055 None:intergenic 45.0%
! CCTATTGGGACCAGAGTTTT+CGG - chr5.2:1655003-1655022 MS.gene21783:intron 45.0%
! GTGAAGGAAGCACATCCTAA+TGG - chr5.2:1654853-1654872 MS.gene21783:intron 45.0%
!! TAATAAAAATATTTATTAAG+TGG + chr5.2:1654324-1654343 None:intergenic 5.0%
AGGAACTGGTCCGAAAACTC+TGG + chr5.2:1655016-1655035 None:intergenic 50.0%
ATGCCAAAGGGTAGGTGTGA+TGG - chr5.2:1654925-1654944 MS.gene21783:intron 50.0%
CCGAAAACTCTGGTCCCAAT+AGG + chr5.2:1655006-1655025 None:intergenic 50.0%
GACAAACAAGGGCAGCCTAT+TGG - chr5.2:1654988-1655007 MS.gene21783:intron 50.0%
GACCAGTTCCTCATGAGTTG+AGG - chr5.2:1655025-1655044 MS.gene21783:intron 50.0%
GCATGTCATCTCATGTGACG+TGG + chr5.2:1654440-1654459 None:intergenic 50.0%
GGATTAAGCAACAGTTCCCC+TGG - chr5.2:1654406-1654425 MS.gene21783:intron 50.0%
GTTCGTGCAGCAATGCCAAA+GGG - chr5.2:1654913-1654932 MS.gene21783:intron 50.0%
TGCCTCAACTCATGAGGAAC+TGG + chr5.2:1655030-1655049 None:intergenic 50.0%
TGTTCGTGCAGCAATGCCAA+AGG - chr5.2:1654912-1654931 MS.gene21783:intron 50.0%
!! GAGGACTGTTCTGAAGGTTG+TGG - chr5.2:1654744-1654763 MS.gene21783:CDS 50.0%
CTGCCATCACACCTACCCTT+TGG + chr5.2:1654931-1654950 None:intergenic 55.0%
GTAAGCCGTTCTCTGTTCGG+TGG - chr5.2:1654385-1654404 MS.gene21783:intron 55.0%
GTGCAGCAATGCCAAAGGGT+AGG - chr5.2:1654917-1654936 MS.gene21783:intron 55.0%
GGGTAGGTGTGATGGCAGTG+AGG - chr5.2:1654933-1654952 MS.gene21783:intron 60.0%
GGTGATGTGCATACGCGCGT+TGG - chr5.2:1654786-1654805 MS.gene21783:CDS 60.0%
! ATGTGCATACGCGCGTTGGC+AGG - chr5.2:1654790-1654809 MS.gene21783:CDS 60.0%
! TGTGCATACGCGCGTTGGCA+GGG - chr5.2:1654791-1654810 MS.gene21783:CDS 60.0%
! CGCGCGTTGGCAGGGAAGAA+AGG - chr5.2:1654799-1654818 MS.gene21783:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 1653954 1655095 1653954 ID=MS.gene21783
chr5.2 mRNA 1653954 1655095 1653954 ID=MS.gene21783.t1;Parent=MS.gene21783
chr5.2 exon 1655057 1655095 1655057 ID=MS.gene21783.t1.exon1;Parent=MS.gene21783.t1
chr5.2 CDS 1655057 1655095 1655057 ID=cds.MS.gene21783.t1;Parent=MS.gene21783.t1
chr5.2 exon 1654741 1654836 1654741 ID=MS.gene21783.t1.exon2;Parent=MS.gene21783.t1
chr5.2 CDS 1654741 1654836 1654741 ID=cds.MS.gene21783.t1;Parent=MS.gene21783.t1
chr5.2 exon 1653954 1654328 1653954 ID=MS.gene21783.t1.exon3;Parent=MS.gene21783.t1
chr5.2 CDS 1653954 1654328 1653954 ID=cds.MS.gene21783.t1;Parent=MS.gene21783.t1
Gene Sequence

>MS.gene21783

ATGCCTTTTCATCGGAAGTTAAAATGTCAACTTCCCCAGGTAAGAGAATAACTGTTTTCTATGTCTTGCAAAACATTGATTCTATCCTTTTGGTGGTGATGTCCAAATTGTTAGTTTACTAAAACCATGTTGTAGTTTCATACTTTCATTATTACATGGTCATTTTGCTCCCTAAAACATGAGCATTGGCAAACTGTTGCTTTTGCAGTAAATTGAAACCACTACATTTTGCAACCACTCTGAAATTTCATATATTAAGCTGCATGACTCTATCTTGCCAGTTTGCACTTGTGCCACTAATCACTCCCTCTATTTACAAGTCAAACACGAGCTCAAGAATTTTTATGTTGATAAGGTGATACTTTATCCACTTAATAAATATTTTTATTAACTTATTAGTTTTTTGTTTCATGTGTTGAAAAACCAAACTAGTAAGCCGTTCTCTGTTCGGTGGATTAAGCAACAGTTCCCCTGGTCTATTTACCACGTCACATGAGATGACATGCAACAGTTATTTTTCTCAGGTACATGTAGGAATCAGTTGCAGGATCATGTTTCAATTATTATAATTTCCTTAACATTATTTTCTTATGCTTGCCAAACACAGTATTGGTAATCTTAAACCTAGATTGTAGAAAACAACAGTAATTTTTCCATGCAGTGCAAAGATACATGTGTTCTTAAAATGAGTCACAAATCCATGCTTAGTTTCTTACACACATTGTTATGATTCCTTCCTGAAGAAAACAATGTCTTCCTTTTTGCAGCAACAACGAACCTTCATACAGATGAGGACTGTTCTGAAGGTTGTGGACAATTCTGGAGCTAAAAAGGTGATGTGCATACGCGCGTTGGCAGGGAAGAAAGGAGCAAGATTAGGTGATATGATACAAGCATCAGTGAAGGAAGCACATCCTAATGGAAAAGTGAAGAAGGGAGCTGTTGTATTTGCCGTAGTTGTTCGTGCAGCAATGCCAAAGGGTAGGTGTGATGGCAGTGAGGTCAAGTTTGACGACAATGCTGTTGTGCTTGTTGACAAACAAGGGCAGCCTATTGGGACCAGAGTTTTCGGACCAGTTCCTCATGAGTTGAGGCAGAAGAAGCATGTCAAGATCCTTACCTTAGCTGGGCATATTGCATAG

Protein sequence

>MS.gene21783.t1

MPFHRKLKCQLPQLHDSILPVCTCATNHSLYLQVKHELKNFYVDKQQRTFIQMRTVLKVVDNSGAKKVMCIRALAGKKGARLGDMIQASVKEAHPNGKVKKGAVVFAVVVRAAMPKGRCDGSEVKFDDNAVVLVDKQGQPIGTRVFGPVPHELRQKKHVKILTLAGHIA